Microbiological research最新文献

{"title":"Effects of bacterial extracellular vesicles derived from oral and gastrointestinal pathogens on systemic diseases","authors":"Han Zhang ,&nbsp;Yunhe Lin ,&nbsp;Siwei Li ,&nbsp;Jiaming Bi ,&nbsp;Jiawei Zeng ,&nbsp;Chuzi Mo ,&nbsp;Shuaimei Xu ,&nbsp;Bo Jia ,&nbsp;Yu Lu ,&nbsp;Chengxia Liu ,&nbsp;Zhongjun Liu","doi":"10.1016/j.micres.2024.127788","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127788","url":null,"abstract":"<div><p>Oral microbiota and gastrointestinal microbiota, the two largest microbiomes in the human body, are closely correlated and frequently interact through the oral-gut axis. Recent research has focused on the roles of these microbiomes in human health and diseases. Under normal conditions, probiotics and commensal bacteria can positively impact health. However, altered physiological states may induce dysbiosis, increasing the risk of pathogen colonization. Studies suggest that oral and gastrointestinal pathogens contribute not only to localized diseases at their respective colonized sites but also to the progression of systemic diseases. However, the mechanisms by which bacteria at these local sites are involved in systemic diseases remain elusive. In response to this gap, the focus has shifted to bacterial extracellular vesicles (BEVs), which act as mediators of communication between the microbiota and the host. Numerous studies have reported the targeted delivery of bacterial pathogenic substances from the oral cavity and the gastrointestinal tract to distant organs via BEVs. These pathogenic components subsequently elicit specific cellular responses in target organs, thereby mediating the progression of systemic diseases. This review aims to elucidate the extensive microbial communication <em>via</em> the oral-gut axis, summarize the types and biogenesis mechanisms of BEVs, and highlight the translocation pathways of oral and gastrointestinal BEVs <em>in vivo</em>, as well as the impacts of pathogens-derived BEVs on systemic diseases.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141244269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought-tolerant rhizobacteria with predicted functional traits enhanced wheat growth and P uptake under moderate drought and low P-availability","authors":"Bouchra Benmrid ,&nbsp;Cherki Ghoulam ,&nbsp;Ibnyasser Ammar ,&nbsp;Dounia Nkir ,&nbsp;Rym Saidi ,&nbsp;Alessia Staropoli ,&nbsp;Giuseppina Iacomino ,&nbsp;Ezzoubair ELhajjami ,&nbsp;Said Cheto ,&nbsp;Joerg Geistlinger ,&nbsp;Mohamed Idbella ,&nbsp;Adnane Bargaz","doi":"10.1016/j.micres.2024.127795","DOIUrl":"10.1016/j.micres.2024.127795","url":null,"abstract":"<div><p>This study aims to investigate the effect of isolated drought-tolerant rhizobacteria, spanning various groups, such as nitrogen-fixing bacteria (NFB), phosphate solubilizing bacteria (PSB), and other plant growth promoting rhizobacteria (PGPR), on the growth of wheat (<em>Triticum durum</em>) plants, focusing on various morphological and physiological responses under moderate drought and low-P availability. Among 343 rhizobacterial morphotypes, 16 exhibited tolerance to NaCl and PEG-6000. These included 8 PSB, 4 NFB, and 4 osmotolerant-PGPR groups, distributed across 14 different genera. Biochemical characterization showcased diverse PGP capabilities, particularly in P solubilization. The dynamic responses of drought-tolerant PSB to salt and PEG-6000-induced drought stress involved variations in organic acid (OA) secretion, with specific acids, including palmitic, lactic, and stearic, playing crucial roles in enhancing available P fractions. Inoculation with rhizobacteria significantly increased both shoot (SDW) and root (RDW) dry weights of wheat plants, as well as rhizosphere available P. PSB₁₁ (<em>Arthrobacter oryzae</em>) emerged as the most effective strain, plausibly due to its positive impact on root morphological traits (length, surface, and volume). Other isolates, PSB₁₀ (<em>Priestia flexa</em>), PSB₁₃ (<em>Bacillus haynesii</em>), and particularly PGPR₂ (<em>Arthrobacter pascens</em>) significantly increased shoot P content (up to 68.91 %), with a 2-fold increase in chlorophyll content. The correlation analysis highlighted positive associations between SDW, shoot P content, chlorophyll content index (CCI), and leaf area. Additionally, a negative correlation emerged between microbial biomass P and root morphophysiological parameters. This pattern could be explained by reduced competition between plants and rhizobacteria for accessible P, as indicated by low microbial biomass P and strong plant growth. Our investigation reveals the potential of drought-tolerant rhizobacteria in enhancing wheat resilience to moderate drought and low-P conditions. This is demonstrated through exceptional performance in influencing root architecture, P utilization efficiency, and overall plant physiological parameters. Beyond these outcomes, the innovative isolation procedure employed, targeting rhizobacteria from diverse groups, opens new avenues for targeted isolation techniques. This unique approach contributes to the novelty of our study, offering promising prospects for targeted bioinoculants in mitigating the challenges of drought and P deficiency in wheat cultivation.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inappropriate use of antibiotic enhances antibiotic resistance dissemination in ESBL-EC: Role of ydcz in outer membrane vesicles biogenesis and protein transport","authors":"Jinpeng Li ,&nbsp;Chao Li ,&nbsp;Chang Tan ,&nbsp;Heting Xu ,&nbsp;Yun Han ,&nbsp;Yulian Hu ,&nbsp;Jian Yang ,&nbsp;Yizhi Tang ,&nbsp;Changwei Lei ,&nbsp;Hongning Wang","doi":"10.1016/j.micres.2024.127774","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127774","url":null,"abstract":"<div><p>Extended-spectrumβ-lactam producing <em>Escherichia coli</em> (ESBL-EC) readily colonizes live poultry and serves as a major source of contamination in retail chicken meat, posing significant threats to public health. This study aims to investigate the impact of inappropriate antibiotic use on the dissemination and exacerbation of antibiotic resistance in ESBL-EC and explore the underlying molecular mechanisms. Through experimental analysis, we propose a hypothesis that inappropriate antibiotic use may exacerbate resistance by affecting vesicle formation and protein secretion. Experimental results demonstrate that under the influence of amoxicillin, the concentration of proteins secreted in outer membrane vehicles (OMVs) by ESBL-EC significantly increases, along with a significant upregulation in the expression of the CTX-M-55-type Extended-spectrum beta-lactamase (CTX-M-55). Proteomic analysis and differential gene knockout experiments identified the key protein YdcZ, associated with OMVs formation and protein transportation in ESBL-EC under amoxicillin treatment. Further investigations reveal direct interactions between YdcZ and other proteins (YdiH and BssR). Upon <em>ydcz</em> gene knockout, a significant decrease in protein concentration within OMVs is observed, accompanied by a noticeable reduction in protection against sensitive bacteria. These findings suggest a critical role of YdcZ in regulating the process of protein transportation to OMVs in ESBL-EC under the influence of amoxicillin. In summary, our research uncovers the significant role of inappropriate antibiotic use in promoting the secretion of OMVs by ESBL-EC, aiding the survival of antibiotic-sensitive bacteria in the vicinity of infection sites. These findings provide new insights into the mechanisms underlying antibiotic-induced bacterial resistance dissemination and offer novel avenues for exploring prevention and control strategies against bacterial resistance propagation.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141244271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The microbial revolution: Unveiling the benefits of vaginal probiotics and prebiotics","authors":"Roshani Pagar ,&nbsp;Sanjeevani Deshkar ,&nbsp;Jayashri Mahore ,&nbsp;Vinita Patole ,&nbsp;Hemant Deshpande ,&nbsp;Nageswari Gandham ,&nbsp;Shahzad Mirza ,&nbsp;Manisha Junnarkar ,&nbsp;Neelu Nawani","doi":"10.1016/j.micres.2024.127787","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127787","url":null,"abstract":"<div><p>Vaginal health is essential to a woman's overall well-being, as abnormalities in vaginal health can lead to a variety of gynaecological disorders, such as urinary tract infections, yeast infections, and bacterial vaginosis. The vaginal microbiome is essential for the prevention of these infections. Disruptions in this microbial ecosystem can significantly impact vaginal health. The concept of utilizing probiotics and prebiotics to stimulate the growth of protective vaginal microbiota has gathered substantial interest in recent years. Probiotics are live micro-organisms that strengthen and restore vaginal microbial balance by lowering pH levels, production of bacteriocins, biofilm disruption, modulation of immune response, and production of hydrogen peroxide (H₂O₂), consequently combating the development of pathogens. Prebiotics are oligosaccharides that encourage the development of probiotics such as lactobacilli species. Probiotics and prebiotics also have some broader implications for vaginal health, including their role in minimizing the incidence of premature birth, optimizing fertility, managing menopausal symptoms, and preventing vaginal infections. Synbiotics are a combination of probiotics and prebiotics that deliver additional benefits by encouraging the development and activity of beneficial microbes. Furthermore, postbiotics are bioactive compounds derived from probiotic bacteria during fermentation that have immunomodulatory actions and provide an additional layer of protection against vaginal infections. The present study highlights the most prevalent vaginal infections and limitations of existing therapies that influence the vaginal microbiota. The profound consequences of probiotics and prebiotics in women's health, including their role in minimizing the prevalence of vaginal infections and promoting overall vaginal health, as well as advanced therapeutic strategies such as synbiotics and postbiotics, are also discussed. The literature offers significant insights into the mechanism, efficacy, and safety of probiotics and prebiotics to healthcare providers and researchers.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141291919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Bifidobacterium animalis subsp. lactis BB-12 and yogurt on mice during oral antibiotic administration","authors":"Ruchita G. Uttarwar ,&nbsp;Solomon A. Mekonnen ,&nbsp;Wannes Van Beeck ,&nbsp;Aidong Wang ,&nbsp;Peter Finnegan ,&nbsp;Robert F. Roberts ,&nbsp;Daniel Merenstein ,&nbsp;Carolyn M. Slupsky ,&nbsp;Maria L. Marco","doi":"10.1016/j.micres.2024.127794","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127794","url":null,"abstract":"<div><p>Probiotics have the potential to prevent disruptions to normal gastrointestinal function caused by oral antibiotic use. In this study, we examined the capacity of <em>Bifidobacterium animalis</em> subspecies <em>lactis</em> BB-12 (BB-12) and yogurt, separately and combined, to mitigate the effects of the antibiotic amoxicillin-clavulanate (AMC) on the gut microbiota and metabolomes of C57BL/6 J mice. Male and female mice were administered either BB-12, yogurt, BB-12 in yogurt, or saline for 10 days concurrent with the inclusion of AMC in the drinking water. Male mice exposed to AMC exhibited significant reductions (p&lt;0.05) in body weight over the course of the study compared to sham (no AMC) controls whereas no such effects were observed for female mice. AMC administration resulted in rapid alterations to the intestinal microbiota in both sexes irrespective of BB-12 or yogurt treatment, including significant (p&lt;0.05) losses in bacterial cell numbers and changes in microbial alpha-diversity and beta-diversity in the feces and cecal contents. The effects of AMC on the gut microbiota were observed within one day of administration and the bacterial contents continued to change over time, showing a succession marked by rapid reductions in <em>Muribaculaceae</em> and <em>Lachnospiraceae</em> and temporal increases in proportions of <em>Acholeplasmataceae</em> (day 1) and <em>Streptococcaceae</em> and <em>Leuconostocaceae</em> (day 5)<em>.</em> By day 10 of AMC intake, high proportions of Gammaproteobacteria assigned as <em>Erwiniaceae</em> or <em>Enterobacteriaceae</em> (average of 63 %), were contained in the stools and were similarly enriched in the cecum. The cecal contents of mice given AMC harbored significantly reduced concentrations of (branched) short-chain fatty acids (SCFA), aspartate, and other compounds, whereas numerous metabolites, including formate, lactate, and several amino acids and amino acid derivatives were significantly enriched. Despite the extensive impact of AMC, starting at day 7 of the study, the body weights of male mice given yogurt or BB-12 (in saline) with AMC were similar to the healthy controls. BB-12 (in saline) and yogurt intake was associated with increased <em>Streptococcaceae</em> and both yogurt and BB-12 resulted in lower proportions of <em>Erwiniaceae</em> in the fecal and cecal contents. The cecal contents of mice fed BB-12 in yogurt contained levels of formate, glycine, and glutamine that were equivalent to the sham controls. These findings highlight the potential of BB-12 and yogurt to mitigate antibiotic-induced gut dysbiosis.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324001952/pdfft?md5=f8e4c73d84037b9f866bade406b4a999&pid=1-s2.0-S0944501324001952-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141291922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary α-linolenic acid supplementation enhances resistance to Salmonella Typhimurium challenge in chickens by altering the intestinal mucosal barrier integrity and cecal microbes","authors":"Boheng Ma ,&nbsp;De Wang ,&nbsp;Xuan Chen ,&nbsp;Qin Wang ,&nbsp;Tiejun Zhang ,&nbsp;Renqiao Wen ,&nbsp;Ming Yang ,&nbsp;Cui Li ,&nbsp;Changwei Lei ,&nbsp;Hongning Wang","doi":"10.1016/j.micres.2024.127773","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127773","url":null,"abstract":"<div><p><em>Salmonella</em> is an important foodborne pathogen. Given the ban on the use of antibiotics during the egg-laying period in China, finding safe and effective alternatives to antibiotics to reduce <em>Salmonella enterica</em> subsp. <em>enterica</em> serovar Typhimurium (<em>S</em>. Typhimurium) infections in chickens is essential for the prevention and control of this pathogen and the protection of human health. Numerous studies have shown that unsaturated fatty acids have a positive effect on intestinal inflammation and resistance to infection by intestinal pathogens. Here we investigated the protective effect of α-linolenic acid (ALA) against <em>S</em>. Typhimurium infection in chickens and further explored its mechanism of action. We added different proportions of ALA to the feed and observed the effect of ALA on <em>S</em>. Typhimurium colonization using metagenomic sequencing technology and physiological index measurements. The role of gut flora on <em>S</em>. Typhimurium colonization was subsequently verified by fecal microbiota transplantation (FMT). We found that ALA protects chickens from <em>S</em>. Typhimurium infection by reducing intestinal inflammation through remodeling the gut microbiota, up-regulating the expression of ileocecal barrier-related genes, and maintaining the integrity of the intestinal epithelium. Our data suggest that supplementation of feed with ALA may be an effective strategy to alleviate <em>S</em>. Typhimurium infection in chickens.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141244268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zebrafish gut microbiota composition in response to tick saliva biomolecules correlates with allergic reactions to mammalian meat consumption","authors":"Sandra Díaz-Sánchez ,&nbsp;Rita Vaz-Rodrigues ,&nbsp;Marinela Contreras ,&nbsp;Marta Rafael ,&nbsp;Margarita Villar ,&nbsp;Almudena González-García ,&nbsp;Sara Artigas-Jerónimo ,&nbsp;Christian Gortázar ,&nbsp;José de la Fuente","doi":"10.1016/j.micres.2024.127786","DOIUrl":"10.1016/j.micres.2024.127786","url":null,"abstract":"<div><p>The α-Gal syndrome (AGS) is an IgE-mediated tick borne-allergy that results in delayed anaphylaxis to the consumption of mammalian meat and products containing α-Gal. Considering that α-Gal-containing microbiota modulates natural antibody production to this glycan, this study aimed to evaluate the influence on tick salivary compounds on the gut microbiota composition in the zebrafish (<em>Danio rerio</em>) animal model. Sequencing of 16 S rDNA was performed in a total of 75 zebrafish intestine samples, representing different treatment groups: PBS control, <em>Ixodes ricinus</em> tick saliva, tick saliva non-protein fraction (NPF), tick saliva protein fraction (PF), and tick saliva protein fractions 1–5 with NPF (F1–5). The results revealed that treatment with tick saliva and different tick salivary fractions, combined with α-Gal-positive dog food feeding, resulted in specific variations in zebrafish gut microbiota composition at various taxonomic levels and affected commensal microbial alpha and beta diversities. Metagenomics results were corroborated by qPCR, supporting the overrepresentation of phylum Firmicutes in the tick saliva group, phylum Fusobacteriota in group F1, and phylum Cyanobacteria in F2 and F5 compared to the PBS-control. qPCRs results at genus level sustained significant enrichment of <em>Plesiomonas</em> spp. in groups F3 and F5, <em>Rhizobium</em> spp. in NPF and F4, and <em>Cloacibacterium</em> spp. dominance in the PBS control group. This study provides new results on the role of gut microbiota in allergic reactions to tick saliva components using a zebrafish model of AGS. Overall, gut microbiota composition in response to tick saliva biomolecules may be associated with allergic reactions to mammalian meat consumption in AGS.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324001873/pdfft?md5=4fce15fa4cd14b371376c288827a8651&pid=1-s2.0-S0944501324001873-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141184134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanistic insights of essential oil of Mentha piperita to control Botrytis cinerea and the prospection of lipid nanoparticles to its application","authors":"Juan Mauricio Fuentes ,&nbsp;Ignacio Jofré ,&nbsp;Gonzalo Tortella ,&nbsp;Adalberto Benavides-Mendoza ,&nbsp;María Cristina Diez ,&nbsp;Olga Rubilar ,&nbsp;Paola Fincheira","doi":"10.1016/j.micres.2024.127792","DOIUrl":"10.1016/j.micres.2024.127792","url":null,"abstract":"<div><p><em>Botrytis cinerea</em> is the phytopathogenic fungus responsible for the gray mold disease that affects crops worldwide. Essential oils (EOs) have emerged as a sustainable tool to reduce the adverse impact of synthetic fungicides. Nevertheless, the scarce information about the physiological mechanism action and the limitations to applying EOs has restricted its use. This study focused on elucidating the physiological action mechanisms and prospection of lipid nanoparticles to apply EO of <em>Mentha piperita</em>. The results showed that the EO of <em>M. piperita</em> at 500, 700, and 900 μL L⁻¹ inhibited the mycelial growth at 100 %. The inhibition of spore germination of <em>B. cinerea</em> reached 31.43 % at 900 μL L⁻¹. The EO of <em>M. piperita</em> decreased the dry weight and increased pH, electrical conductivity, and cellular material absorbing OD_260 nm of cultures of <em>B. cinerea</em>. The fluorescence technique revealed that EO reduced hyphae width, mitochondrial activity, and viability, and increased ROS production. The formulation of EO of <em>M. piperita</em> loaded- solid lipid nanoparticles (SLN) at 500, 700, and 900 μL L⁻¹ had particle size ∼ 200 nm, polydispersity index &lt; 0.2, and stability. Also, the thermogravimetric analysis indicated that the EO of <em>M. piperita</em>-loaded SLN has great thermal stability at 50 °C. EO of <em>M. piperita</em>-loaded SLN reduced the mycelial growth of <em>B. cinerea</em> by 70 %, while SLN formulation (without EO) reached 42 % inhibition. These results supported that EO of <em>M. piperita</em>-loaded SLN is a sustainable tool for reducing the disease produced by <em>B. cinerea</em>.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141189270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the influence of a probiotic combination of Heyndrickxia coagulans and Lacticaseibacillus casei on healthy human gut microbiota using the TripleSHIME® system","authors":"Elizabeth Goya-Jorge ,&nbsp;Irma Gonza ,&nbsp;Pauline Bondue ,&nbsp;Germain Druart ,&nbsp;Mohamed Al-Chihab ,&nbsp;Samiha Boutaleb ,&nbsp;Caroline Douny ,&nbsp;Bernard Taminiau ,&nbsp;Georges Daube ,&nbsp;Marie-Louise Scippo ,&nbsp;Philippe Thonart ,&nbsp;Véronique Delcenserie","doi":"10.1016/j.micres.2024.127778","DOIUrl":"10.1016/j.micres.2024.127778","url":null,"abstract":"<div><p>Probiotics are host-friendly microorganisms that can have important health benefits in the human gut microbiota as dietary supplements. Maintaining a healthy gut microbial balance relies on the intricate interplay among the intestinal microbiota, metabolic activities, and the host's immune response. This study aims to explore if a mixture of <em>Heyndrickxia coagulans</em> [ATB-BCS-042] and <em>Lacticaseibacillus casei</em> [THT-030-401] promotes <em>in vitro</em> this balance in representative gut microbiota from healthy individuals using the Triple-SHIME® (Simulation of the Human Intestinal Microbial Ecosystem). Metataxonomic analysis of the intestinal microbes revealed that the probiotic mix was not causing important disruptions in the biodiversity or microbial composition of the three simulated microbiota. However, some targeted populations analyzed by qPCR were found to be disrupted at the end of the probiotic treatment or after one week of washout. Populations such as Cluster IV, Cluster XVIa, and <em>Roseburia</em> spp., were increased indicating a potential gut health-promoting butyrogenic effect of the probiotic supplementation. In two of the systems, bifidogenic effects were observed, while in the third, the treatment caused a decrease in bifidobacteria. For the health-detrimental biomarker <em>Escherichia-Shigella</em>, a mild decrease in all systems was observed in the proximal colon sections, but these genera were highly increased in the distal colon sections. By the end of the washout, <em>Bacteroides-Prevotella</em> was found consistently boosted, which could have inflammatory consequences in the intestinal context. Although the probiotics had minimal influence on most quantified metabolites, ammonia consistently decreased after one week of daily probiotic supplementation. In reporter gene assays, aryl hydrocarbon receptor (AhR) activation was favored by the metabolic output obtained from post-treatment periods. Exposure of a human intestinal cell model to fermentation supernatant obtained after probiotic supplementation induced a trend to decrease the mRNA expression of immunomodulatory cytokines (IL-6, IL-8). Overall, with some exceptions, a positive impact of <em>H. coagulans</em> and <em>L. casei</em> probiotic mix was observed in the three parallel experiments, despite inter-individual differences. This study might serve as an <em>in vitro</em> pipeline for the impact assessment of probiotic combinations on the human gut microbiota.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-wall-anchored proteins affect invasive host colonization and biofilm formation in Staphylococcus aureus","authors":"Zhenbo Xu ,&nbsp;Yaqin Li ,&nbsp;Aijuan Xu ,&nbsp;Thanapop Soteyome ,&nbsp;Lei Yuan ,&nbsp;Qin Ma ,&nbsp;Gamini Seneviratne ,&nbsp;Xuejie Li ,&nbsp;Junyan Liu","doi":"10.1016/j.micres.2024.127782","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127782","url":null,"abstract":"<div><p>As a major human and animal pathogen, <em>Staphylococcus aureus</em> can attach to medical implants (abiotic surface) or host tissues (biotic surface), and further establish robust biofilms which enhances resistance and persistence to host immune system and antibiotics. Cell-wall-anchored proteins (CWAPs) covalently link to peptidoglycan, and largely facilitate the colonization of <em>S. aureus</em> on various surfaces (including adhesion and biofilm formation) and invasion into host cells (including adhesion, immune evasion, iron acquisition and biofilm formation). During biofilm formation, CWAPs function in adhesion, aggregation, collagen-like fiber network formation, and consortia formation. In this review, we firstly focus on the structural features of CWAPs, including their intracellular function and interactions with host cells, as well as the functions and ligand binding of CWAPs in different stages of <em>S. aureus</em> biofilm formation. Then, the roles of CWAPs in different biofilm processes with regards in development of therapeutic approaches are clarified, followed by the association between CWAPs genes and clonal lineages. By touching upon these aspects, we hope to provide comprehensive knowledge and clearer understanding on the CWAPs of <em>S. aureus</em> and their roles in biofilm formation, which may further aid in prevention and treatment infection and vaccine development.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141244270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}