Microbiological research最新文献

{"title":"Co-regulation of HexC fine-tunes HexA-dependent transcription and acid stress response in Pectobacterium carotovorum","authors":"Eunhye Goo ,&nbsp;Serim Lee ,&nbsp;Jae Hoon Lee","doi":"10.1016/j.micres.2025.128389","DOIUrl":"10.1016/j.micres.2025.128389","url":null,"abstract":"<div><div>Environmental fluctuations and host-derived stresses demand precise transcriptional regulation in bacterial pathogens to ensure survival and pathogenic success. In <em>Pectobacterium</em> sp., causal agents of soft rot disease, the global regulator HexA is known to repress genes involved in plant cell wall-degrading enzyme (PCWDE) production, quorum sensing (QS), and virulence. However, its broader regulatory scope and physiological significance remain poorly understood. Here, we examined HexA function in <em>P. carotovorum</em> PCC27 through integrated ChIP-seq and RNA-seq analyses, revealing that HexA controls a diverse regulon, including transcriptional regulators, hypothetical proteins, and post-transcriptional regulators such as RsmA and RsmB. Among the newly identified targets, HexC, previously annotated as a hypothetical protein, was identified as a co-regulator that physically interacts with HexA and increases its DNA-binding affinity. Biochemical and genetic analyses demonstrated that HexC reinforces HexA-mediated transcriptional repression. The <em>hexA</em>/<em>hexC</em> double mutant produced higher levels of QS signals than the <em>hexA</em> single mutant but did not exhibit further increase in virulence. Phenotypic assays showed that the <em>hexA</em>/<em>hexC</em> mutant exhibited reduced fitness under acidic conditions yet outcompeted the wild-type at neutral pH in LB medium. However, <em>in planta</em>, the <em>hexA</em>, <em>hexC</em>, and <em>hexA</em>/<em>hexC</em> mutants consistently displayed reduced competitiveness compared to the wild-type in both single and mixed infections. These findings establish HexC as a co-repressor that fine-tunes HexA activity, providing mechanistic insights into how transcriptional regulation evolves to balance virulence and environmental fitness.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128389"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145466791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteroides acidifaciens alleviates high-fat diet-induced obesity-related osteoporosis by regulating gut microbiota and bile acid metabolism via the gut-bone axis","authors":"Cihua Zheng ,&nbsp;Jian Xie ,&nbsp;Furui Tang ,&nbsp;Zhuoya Wang ,&nbsp;Li Liu ,&nbsp;Yimin Pi ,&nbsp;Yuchun Zhong ,&nbsp;Zhidong He ,&nbsp;Tian Liu ,&nbsp;Jiacheng Zheng ,&nbsp;Jun Luo","doi":"10.1016/j.micres.2025.128393","DOIUrl":"10.1016/j.micres.2025.128393","url":null,"abstract":"<div><div>Obesity disrupts bone formation-resorption balance. Despite the established role of <em>Bacteroides acidifaciens</em> (<em>B. acidifaciens</em>) in modulating metabolic homeostasis, reducing inflammation, and improving lipid metabolism, its impact on obesity-associated osteoporosis is still not well understood. In this study, we delved into the potential protective influence of <em>B. acidifaciens</em> on high-fat diet (HFD) induced bone loss. The results showed that <em>B. acidifaciens</em> sharply improved weight gain, glucose and lipid metabolism in 16 weeks HFD mice. Both <em>In vitro</em> and <em>in vivo</em> experiments have conclusively demonstrated that the introduction of <em>B. acidifaciens</em> notably ameliorated the imbalance of HFD induced osteogenesis and osteoclastogenesis. <em>B. acidifaciens</em> also regulated HFD induced gut microbiota and bile acid metabolism, and strengthened intestinal mucosal barrier function. Additionally, <em>B. acidifaciens</em> significantly activated the AMPK-PPARα signaling pathway in bone tissue. Thus, our study indicated that <em>B. acidifaciens</em> regulated metabolism, restored gut microbiota balance, and activated AMPK-PPARα pathway to prevent HFD-induced bone loss, potentially aiding in preventing and treating obesity-related osteoporosis.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128393"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145466792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay between glucose and aromatic compound regulation by two IclR-type transcription factors, LigR1 and LigR2, in Pseudomonas putida KT2440","authors":"Elina Kadriu,&nbsp;Sophie Qin,&nbsp;Stephanie M. Prezioso,&nbsp;Dinesh Christendat","doi":"10.1016/j.micres.2025.128382","DOIUrl":"10.1016/j.micres.2025.128382","url":null,"abstract":"<div><div>Carbon utilization strategies are fundamental to microbial proliferation within complex ecosystems like the soil microbiome. These strategies dictate how microbes prioritize, and metabolize available carbon compounds, shaping community dynamics and ecological outcomes. <em>Pseudomonas putida</em> KT2440, a soil bacterium renowned for its metabolic versatility, exemplifies this adaptive capacity. However, the regulatory mechanism it employs to prioritize sugars vs aromatic compounds for their energy requirement remains poorly understood. Here, we investigated two IclR-type transcriptional regulators, LigR1 and LigR2, which control expression of the <em>lig1</em> and <em>lig2</em> operons. Functional analyses reveal that LigR1 and LigR2 activate <em>lig1</em> but repress the <em>lig2</em> operon. 4-hydroxybenzoate binding to LigR1 represses gene expression, whereas quinate, protocatechuate, and 4-hydroxybenzoate bind to LigR2 to induce <em>lig2</em> operon expression. Additionally, <em>ligR1</em> deletion causes growth defects on glucose and 4-hydroxybenzoate, accompanied by cell elongation and aggregation. We propose that the <em>lig1</em> operon mediates dual influx of glucose and aromatics via its major facilitator superfamily transporter, while the <em>lig2</em> operon catalyzes aromatic breakdown through a protocatechuate intermediate and meta-cleavage pathway, supplying oxaloacetate to the TCA cycle. Importantly, <em>P. putida</em> prioritizes shikimate pathway intermediates as energy sources under specific metabolic conditions, such as their accumulation. Overall, these findings redefine the metabolic flexibility of soil pseudomonads and reveal a novel mechanism for thriving in chemically diverse environments. By illuminating a dual regulatory system, our study offers new insight into microbial carbon flux and on the traditional biosynthetic paradigm of the shikimate pathway, revealing its unexpected role in supplying the organism with energy generating compounds.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128382"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145418811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The gut microbiota in post-stroke depression: A systematic review of microbial mechanisms and therapeutic targeting of neuroinflammation","authors":"Qianwen Zhang ,&nbsp;Shiqing Zhang ,&nbsp;Xingqin Cao ,&nbsp;Yinghao Zhi ,&nbsp;Ying Guo","doi":"10.1016/j.micres.2025.128391","DOIUrl":"10.1016/j.micres.2025.128391","url":null,"abstract":"<div><div>Post-stroke depression (PSD), a frequent and debilitating complication after stroke, severely hinders rehabilitation. Emerging evidence underscores the role of neuroinflammation and the gut microbiota in PSD pathogenesis. This review systematically elaborates the mechanisms by which gut dysbiosis contributes to PSD-related neuroinflammation via immune cell regulation (e.g., Treg/Th17 balance), microbial metabolites (e.g., SCFAs, tryptophan derivatives), and neural pathways (vagus nerve, HPA axis). A key focus is the comparative analysis of the gut microbiota in PSD against major depressive disorder (MDD) and Alzheimer's disease (AD), revealing a unique, stroke-induced microbial signature characterized by a loss of protective symbionts and a bloom of pro-inflammatory taxa. We further discuss the translational potential of microbiota-targeted interventions (e.g., probiotics, prebiotics) for PSD. By integrating clinical microbial ecology with mechanistic insights, this review synthesizes evidence suggesting that the gut microbiome may represent a promising diagnostic and therapeutic target for PSD, offering a distinct perspective from previous literature.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128391"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome based precision medicine through integrated multiomics and machine learning","authors":"Abhishek Kumar ,&nbsp;Caiming Xu ,&nbsp;Tikam Chand Dakal","doi":"10.1016/j.micres.2025.128384","DOIUrl":"10.1016/j.micres.2025.128384","url":null,"abstract":"<div><div>Gut microbiome (GME) is a dynamic ecosystem composed of diverse microorganisms with extensive functional potential that influence host physiology, endocrinology, and neurology. This review explores how multiomics (m^OMICS) and machine learning (ML) enhance understanding of the GME and its implications for human disease and therapy. Integrating metagenomics, metatranscriptomics, metaproteomics, and metabolomics with ML enables the linkage of microbial composition and function to clinical outcomes. Combined m^OMICS approaches elucidate species and strain dynamics, metabolic pathways, and metabolite production within the gut environment. Techniques such as shotgun metagenomics, metagenome-assembled genomes, and pathway mapping reveal associations between dysbiosis and diseases including inflammatory bowel disease, colorectal cancer, cardiometabolic, and neurological disorders. Mechanistic insights highlight short-chain fatty acids in immune regulation, bile acid transformations in metabolic signaling, and trimethylamine N-oxide in cardiovascular risk. ML models trained on heterogeneous datasets identify disease-related microbial modules, improve patient stratification, and predict therapeutic responses, such as differentiating IBD subtypes and detecting cancer-linked microbial signatures. Network analyses uncover gut microbial interaction patterns influencing host physiology. Emerging integrative tools like MOFA+ , DIABLO, and MintTea strengthen cross-modal analysis and biomarker discovery. Standardized workflows addressing quality control, assembly, binning, annotation, and visualization ensure reproducibility. Together, m^OMICS and ML establish a robust framework for translating GME ecology into clinically relevant biomarkers and precision interventions. To enhance reliability, GME studies should adopt uniform sampling protocols, correct compositional biases, employ interpretable models, and validate findings across multi-site cohorts to advance microbiome-based diagnostics and therapeutics in precision medicine.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128384"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic rewiring and morphological adaptations drive bacterial strain-specific cadmium defense in the Yangtze River estuary","authors":"Wensi Zhang ,&nbsp;Qian Liu ,&nbsp;Bharat Manna ,&nbsp;Naresh Singhal ,&nbsp;Jian Wang ,&nbsp;Boyu Lyu ,&nbsp;Xueyang Zhou ,&nbsp;Yurong Qian","doi":"10.1016/j.micres.2025.128326","DOIUrl":"10.1016/j.micres.2025.128326","url":null,"abstract":"<div><div>Cadmium (Cd) contamination in coastal regions poses severe environmental risks, yet bacterial defense mechanisms against Cd remain poorly understood. This study unveils distinct tolerant strategies of two highly Cd-tolerant bacteria isolated from the Yangtze River estuary: <em>Comamonas</em> sp. Y49 and <em>Aeromonas</em> sp. Y23. We exposed two bacterial strains to Cd^2 + concentrations ranging from sub-lethal to near-lethal levels, based on their minimum inhibitory concentrations, to investigate their stress response mechanisms. The cellular adaptations were comprehensively analyzed through transcriptomic profiling and scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDS). Transcriptomic analyses revealed that both strains significantly stimulated carbon, nitrogen and sulfur metabolism under Cd stress for maintaining essential substance and energy resources. They both enhanced reactive oxygen scavenger and polyamine biosynthesis gene regulation, suggesting a shared strategy for mitigating oxidative stress. Strain <em>Comamonas</em> sp. Y49 showed a 2.97-fold increase in metal efflux gene regulation and secreted extracellular polysaccharide-like substances with SEM-EDS detecting 0.50 % Cd on cell surfaces, while <em>Aeromonas</em> sp. Y23 potentially reduced Cd uptake by forming long-chain cellular structures. Besides, <em>Comamonas</em> sp. Y49 downregulated motility genes by 2.07-fold, while <em>Aeromonas</em> sp. Y23 upregulated them by 1.12-fold, indicating divergent biofilm formation strategies. This study provides novel insights into bacterial Cd resistance, revealing strain-specific adaptive mechanisms that combine metabolic rewiring, morphological changes, and molecular defense strategies. Our findings provide valuable insights on bacterial adaptations to metal stress and establish a molecular foundation for developing microbial-based strategies to address metal contamination in estuarine environments.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128326"},"PeriodicalIF":6.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel plant-derived compounds modulate gut microbiome dysbiosis in colitis mice: A potential therapeutic avenue for inflammatory bowel disease","authors":"Md. Mizanur Rahaman ,&nbsp;Karma Yeshi ,&nbsp;Mehedi Hasan Bappi ,&nbsp;Md. Zohorul Islam ,&nbsp;Phurpa Wangchuk ,&nbsp;Subir Sarker","doi":"10.1016/j.micres.2025.128343","DOIUrl":"10.1016/j.micres.2025.128343","url":null,"abstract":"<div><div>Inflammatory bowel disease (IBD) is a chronic, multifactorial disorder of the gastrointestinal tract, often associated with dysbiosis in gut microbiota. While the exact cause of IBD remains unclear, alterations in gut microbiome composition and function are recognised as key contributors to IBD pathogenesis. Natural compounds with anti-inflammatory properties are increasingly explored as potential therapeutic options for IBD. This study evaluated the therapeutic effects of two newly isolated galloyl glucosides—galloyl-lawsoniaside A (comp-4) and uromyrtoside (comp-6)—alongside dexamethasone (DEX) on microbiome regulation in a 2, 4, 6-Trinitrobenzene sulfonic acid (TNBS)-induced colitis mouse model. We employed PacBio HiFi full-length 16S rRNA gene sequencing on mouse colon tissue to assess changes in the intestinal microbiome and its associated functional pathways. TNBS-induced colitis significantly altered microbial composition, increasing the abundance of <em>Acutalibacter muris</em>, <em>Monoglobus pectinilyticus</em>, <em>Streptococcus pneumoniae</em>, <em>Parabacteroides merdae</em>, and <em>Haemophilus influenzae</em>, while decreasing <em>Staphylococcus ureilyticus</em> and <em>Mailhella massiliensis</em>. Treatment with comps 4 and 6 effectively restored the imbalanced microbiota. Functional pathway analysis revealed that colitis reduced microbial pathways, including peptidoglycan biosynthesis and the Bifidobacterium shunt. These disruptions were restored following treatment with our plant-derived compounds. Functional improvements were likely associated with reduced IL-6 production and restoring intestinal barrier integrity. Notably, comp-4 exhibited the most pronounced therapeutic efficacy across both microbial and host-associated parameters. <em>In silico</em> docking further supported the anti-inflammatory and immunomodulatory potential of these compounds. Together, our findings highlight the interplay between microbial function and host immunity in IBD and identify plant-derived galloyl glucosides as promising candidates for microbiome-targeted IBD therapeutics.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128343"},"PeriodicalIF":6.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145099372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host phylogeny and traits shape the composition and network structure of the phyllosphere microbial communities in temperate desert plants","authors":"Jun Zhang ,&nbsp;Xiaobing Zhou ,&nbsp;Xiaoying Rong ,&nbsp;Benfeng Yin ,&nbsp;Lei Zhang ,&nbsp;Yuanming Zhang","doi":"10.1016/j.micres.2025.128355","DOIUrl":"10.1016/j.micres.2025.128355","url":null,"abstract":"<div><div>Phyllosphere microorganisms play a vital role in enhancing the adaptability and functionality of their host plants. Although the effects of phyllosphere microbial communities on host functional traits and their association with host phylogeny has been widely investigated, it remains unclear whether host selection consistently drives the assembly of these communities. In this study, bacterial and fungal communities on the surfaces of 734 leaf samples were characterized using bacterial and fungal amplicon sequencing. These microbial communities were associated with 42 plant species native to the Gurbantunggut Desert, a representative temperate desert located in Central Asia. The research assessed the relative contributions of plant-related factors, abiotic environmental variables (such as climate and soil), and spatial components to the observed variation in phyllosphere microbial communities, and further inferred the topological structure of plant-microbe interaction networks. The results indicate that plant phylogeny, plant functional traits, abiotic environment conditions, and spatial factors account for variations in the bacterial community composition (36.4 %, 4.6 %, 1.0 %, and 0.1 %, respectively) and the fungal community composition (28.6 %, 3.0 %, 1.5 %, and 1.2 %, respectively), following a hierarchical trend of plant phylogeny &gt; plant functional traits &gt; abiotic environment &gt; space. Plant phylogeny and functional traits play a central role in shaping the assembly of phyllosphere microbial communities, indicating that plant filtering effects significantly influence microbial composition. Analysis of plant-microbe interactions reveals distinct preferences of microbial taxa for plant hosts across different taxonomic levels and geographic regions. Bipartite network analysis further illustrates that plant-microbe networks are highly specialized and modular, with plant-fungal networks exhibiting greater host specificity compared to plant-bacterial networks. Collectively, these findings underscore plant filtering as the primary determinant of microbial community assembly in the desert phyllosphere and provide valuable insights into the macroecological patterns shaping plant-microbe interactions in arid ecosystems.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128355"},"PeriodicalIF":6.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Gut microbiota-driven BCAA biosynthesis via Staphylococcus aureus -expressed acetolactate synthase impairs glycemic control in type 2 diabetes in South China” [Microbiol. Res. 296 (2025) 128145]","authors":"Tingting Liang ,&nbsp;Tong Jiang ,&nbsp;Zhuang Liang ,&nbsp;Longyan Li ,&nbsp;Ya Chen ,&nbsp;Tong Chen ,&nbsp;Lingshuang Yang ,&nbsp;Ni Zhang ,&nbsp;Bo Dong ,&nbsp;Xinqiang Xie ,&nbsp;Bing Gu ,&nbsp;Qingping Wu","doi":"10.1016/j.micres.2025.128344","DOIUrl":"10.1016/j.micres.2025.128344","url":null,"abstract":"","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128344"},"PeriodicalIF":6.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crosstalk between the m6A modification and the gut microbiota in lipid metabolism","authors":"Haiyan Chen,&nbsp;Yaolin Ren,&nbsp;Jie Yu,&nbsp;Jing Ren,&nbsp;Yuan Zeng,&nbsp;Yifan Wu,&nbsp;Qian Zhang,&nbsp;Xinhua Xiao","doi":"10.1016/j.micres.2025.128356","DOIUrl":"10.1016/j.micres.2025.128356","url":null,"abstract":"<div><div>Lipid metabolism is essential for maintaining cellular homeostasis and human health, and its dysregulation can contribute to metabolic disorders such as obesity and diabetes. As one of the most prevalent RNA modifications, the N6-methyladenosine (m6A) modification plays a pivotal role in regulating gene expression and metabolic pathways. The gut microbiota influences lipid metabolism by modulating the host's m6A modification patterns. Research has shown that the gut microbiota can regulate the levels of the m6A modification in host tissues, while the m6A modification also impacts the composition and functionality of the gut microbiota. This review comprehensively examines the interaction between the m6A modification and the gut microbiota, elucidating its underlying mechanisms in lipid metabolism and highlighting the potential applications of this crosstalk in addressing metabolic diseases. Future investigations should aim to further elucidate the precise molecular mechanisms governing the interplay between the m6A modification and the gut microbiota, thereby providing novel therapeutic targets and strategies for metabolic disease management.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128356"},"PeriodicalIF":6.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}