Microbiological research最新文献

{"title":"Non-antibiotic pharmaceutical phenylbutazone binding to MexR reduces the antibiotic susceptibility of Pseudomonas aeruginosa","authors":"Limin Dong ,&nbsp;Lang Sun ,&nbsp;Yan Yang ,&nbsp;Lin Yuan ,&nbsp;Wei Gao ,&nbsp;Dan Yu ,&nbsp;Qinghong Meng ,&nbsp;Wei Shi ,&nbsp;Qing Wang ,&nbsp;Yue Li ,&nbsp;Youwen Zhang ,&nbsp;Xuefu You ,&nbsp;Kaihu Yao","doi":"10.1016/j.micres.2024.127872","DOIUrl":"10.1016/j.micres.2024.127872","url":null,"abstract":"<div><p>Antimicrobial resistance has been an increasingly serious threat to global public health. The contribution of non-antibiotic pharmaceuticals to the development of antibiotic resistance has been overlooked. Our study found that the anti-inflammatory drug phenylbutazone could protect <em>P. aeruginosa</em> against antibiotic mediated killing by binding to the efflux pump regulator MexR. In this study, antibiotic activity against <em>P. aeruginosa</em> alone or in combination with phenylbutazone was evaluated <em>in vitro</em> and <em>in vivo</em>. Resazurin accumulation assay, transcriptomic sequencing, and PISA assay were conducted to explore the underlying mechanism for the reduced antibiotic susceptibility caused by phenylbutazone. Then EMSA, ITC, molecular dynamic simulations, and amino acid substitutions were used to investigate the interactions between phenylbutazone and MexR. We found that phenylbutazone could reduce the susceptibility of <em>P. aeruginosa</em> to multiple antibiotics, including parts of β-lactams, fluoroquinolones, tetracyclines, and macrolides. Phenylbutazone could directly bind to MexR, then promote MexR dissociating from the <em>mexA</em>-<em>mexR</em> intergenic region and de-repress the expression of MexAB-OprM efflux pump. The overexpressed MexAB-OprM pump resulted in the reduced antibiotic susceptibility. And the His41 and Arg21 residues of MexR were involved in the phenylbutazone-MexR interaction. We hope this study would imply the potential risk of antibiotic resistance caused by non-antibiotic pharmaceuticals.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"288 ","pages":"Article 127872"},"PeriodicalIF":6.1,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002738/pdfft?md5=d387e694ccd025c86c3220491672c988&pid=1-s2.0-S0944501324002738-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141985496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is there a role for intestinal sporobiota in the antimicrobial resistance crisis?","authors":"A. Romero-Rodríguez ,&nbsp;B. Ruíz-Villafán ,&nbsp;S. Sánchez ,&nbsp;D. Paredes-Sabja","doi":"10.1016/j.micres.2024.127870","DOIUrl":"10.1016/j.micres.2024.127870","url":null,"abstract":"<div><p>Antimicrobial resistance (AMR) is a complex issue requiring specific, multi-sectoral measures to slow its spread. When people are exposed to antimicrobial agents, it can cause resistant bacteria to increase. This means that the use, misuse, and excessive use of antimicrobial agents exert selective pressure on bacteria, which can lead to the development of \"silent\" reservoirs of antimicrobial resistance genes. These genes can later be mobilized into pathogenic bacteria and contribute to the spread of AMR. Many socioeconomic and environmental factors influence the transmission and dissemination of resistance genes, such as the quality of healthcare systems, water sanitation, hygiene infrastructure, and pollution. The sporobiota is an essential part of the gut microbiota that plays a role in maintaining gut homeostasis. However, because spores are highly transmissible and can spread easily, they can be a vector for AMR. The sporobiota resistome, particularly the mobile resistome, is important for tracking, managing, and limiting the spread of antimicrobial resistance genes among pathogenic and commensal bacterial species.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"288 ","pages":"Article 127870"},"PeriodicalIF":6.1,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002714/pdfft?md5=44fd0108bb79c30d56d204af9b92bebb&pid=1-s2.0-S0944501324002714-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"‘Altruistic’ cooperation among the prokaryotic community of Atlantic salterns assessed by metagenomics","authors":"Alicia García-Roldán,&nbsp;Rafael R. de la Haba,&nbsp;Cristina Sánchez-Porro,&nbsp;Antonio Ventosa","doi":"10.1016/j.micres.2024.127869","DOIUrl":"10.1016/j.micres.2024.127869","url":null,"abstract":"<div><p>Hypersaline environments are extreme habitats with a limited prokaryotic diversity, mainly restricted to halophilic or halotolerant archaeal and bacterial taxa adapted to highly saline conditions. This study attempts to analyze the taxonomic and functional diversity of the prokaryotes that inhabit a solar saltern located at the Atlantic Coast, in Isla Cristina (Huelva, Southwest Spain), and the influence of salinity on the diversity and metabolic potential of these prokaryotic communities, as well as the interactions and cooperation among the individuals within that community. Brine samples were obtained from different saltern ponds, with a salinity range between 19.5 % and 39 % (w/v). Total prokaryotic DNA was sequenced using the Illumina shotgun metagenomic strategy and the raw sequence data were analyzed using supercomputing services following the MetaWRAP and SqueezeMeta protocols. The most abundant phyla at moderate salinities (19.5–22 % [w/v]) were <em>Methanobacteriota</em> (formerly <em>“Euryarchaeota”</em>), <em>Pseudomonadota</em> and <em>Bacteroidota</em>, followed by <em>Balneolota</em> and <em>Actinomycetota</em> and <em>Uroviricota</em> in smaller proportions, while at high salinities (36–39 % [w/v]) the most abundant phylum was <em>Methanobacteriota,</em> followed by <em>Bacteroidota</em>. The most abundant genera at intermediate salinities were <em>Halorubrum</em> and the bacterial genus <em>Spiribacter</em>, while the haloarchaeal genera <em>Halorubrum</em>, <em>Halonotius</em>, and <em>Haloquadratum</em> were the main representatives at high salinities. A total of 65 MAGs were reconstructed from the metagenomic datasets and different functions and pathways were identified in them, allowing to find key taxa in the prokaryotic community able to synthesize and supply essential compounds, such as biotin, and precursors of other bioactive molecules, like β-carotene, and bacterioruberin, to other dwellers in this habitat, lacking the required enzymatic machinery to produce them. This work shed light on the ecology of aquatic hypersaline environments, such as the Atlantic Coast salterns, and on the dynamics and factors affecting the microbial populations under such extreme conditions.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"288 ","pages":"Article 127869"},"PeriodicalIF":6.1,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002702/pdfft?md5=87f6de3422c645ff0bb9d832c328008e&pid=1-s2.0-S0944501324002702-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141998319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring gut microbial metabolites as key players in inhibition of cancer progression: Mechanisms and therapeutic implications","authors":"Tianxiang Yin ,&nbsp;Xiang Zhang ,&nbsp;Yan Xiong ,&nbsp;Bohao Li ,&nbsp;Dong Guo ,&nbsp;Zhou Sha ,&nbsp;Xiaoyuan Lin ,&nbsp;Haibo Wu","doi":"10.1016/j.micres.2024.127871","DOIUrl":"10.1016/j.micres.2024.127871","url":null,"abstract":"<div><p>The gut microbiota plays a critical role in numerous biochemical processes essential for human health, such as metabolic regulation and immune system modulation. An increasing number of research suggests a strong association between the gut microbiota and carcinogenesis. The diverse metabolites produced by gut microbiota can modulate cellular gene expression, cell cycle dynamics, apoptosis, and immune system functions, thereby exerting a profound influence on cancer development and progression. A healthy gut microbiota promotes substance metabolism, stimulates immune responses, and thereby maintains the long-term homeostasis of the intestinal microenvironment. When the gut microbiota becomes imbalanced and disrupts the homeostasis of the intestinal microenvironment, the risk of various diseases increases. This review aims to elucidate the impact of gut microbial metabolites on cancer initiation and progression, focusing on short-chain fatty acids (SCFAs), polyamines (PAs), hydrogen sulfide (H₂S), secondary bile acids (SBAs), and microbial tryptophan catabolites (MTCs). By detailing the roles and molecular mechanisms of these metabolites in cancer pathogenesis and therapy, this article sheds light on dual effects on the host at different concentrations of metabolites and offers new insights into cancer research.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"288 ","pages":"Article 127871"},"PeriodicalIF":6.1,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery and characterization of the PpqI/R quorum sensing system activated by GacS/A and Hfq in Pseudomonas protegens H78","authors":"Chenxi Nie ,&nbsp;Xianqing Huang ,&nbsp;Tao Xiang,&nbsp;Zheng Wang,&nbsp;Xuehong Zhang","doi":"10.1016/j.micres.2024.127868","DOIUrl":"10.1016/j.micres.2024.127868","url":null,"abstract":"<div><p><em>Pseudomonas protegens</em> can generally produce multiple antibiotics including pyoluteorin (Plt), 2,4-diacetylphloroglucinol (DAPG), and pyrrolnitrin (Prn). In this study, we discovered and characterized a quorum sensing (QS) system, PpqI/R, in <em>P. protegens</em> H78. PpqI/R, encoded by two open reading frames (ORFs) (H78_01960/01961) in <em>P. protegens</em> H78 genome, is a LuxI/R-type QS system. Four long-chain acyl homoserine lactone (AHL) signaling molecules, 3-OH-C₁₀-HSL, 3-OH-C₁₂-HSL, C₁₂-HSL, and 3-OH-C₁₄-HSL, are produced by H78. Biosynthesis of these AHLs is catalyzed by PpqI synthase and activated by the PpqR regulator in H78 and in <em>Escherichia coli</em> when heterologously expressed. PpqR activates <em>ppqI</em> expression by targeting the <em>lux</em> box upstream of the <em>ppqI</em> promoter in cooperation with corresponding AHLs. The four aforementioned AHLs exhibited different capabilities to induce <em>ppqI</em> promoter expression, with 3-OH-C₁₂-HSL showing the highest induction activity. In H78 cells, <em>ppqI/R</em> expression is activated by the two-component system GacS/A and the RNA chaperone Hfq. Differential regulation of the PpqI/R system in secondary metabolism has a negative effect on DAPG biosynthesis and <em>ped</em> operon (involved in volatile organic compound biosynthesis) expression. In contrast, Plt biosynthesis and <em>prn</em> operon expression were positively regulated by PpqI/R. In summary, PpqI/R, the first characterized QS system in <em>P. protegens,</em> is activated by GacS/A and Hfq and controls the expression of secondary metabolites, including antibiotics.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127868"},"PeriodicalIF":6.1,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the role of a novel postbiotic bile acid: Interplay with gut microbiota, modulation of the farnesoid X receptor, and prospects for clinical translation","authors":"Beibei Wang ,&nbsp;Dong Han ,&nbsp;Xinyue Hu ,&nbsp;Jing Chen ,&nbsp;Yuwei Liu ,&nbsp;Jing Wu","doi":"10.1016/j.micres.2024.127865","DOIUrl":"10.1016/j.micres.2024.127865","url":null,"abstract":"<div><p>The gut microbiota, mainly resides in the colon, possesses a remarkable ability to metabolize different substrates to create bioactive substances, including short-chain fatty acids, indole-3-propionic acid, and secondary bile acids. In the liver, bile acids are synthesized from cholesterol and then undergo modification by the gut microbiota. Beyond those reclaimed by the enterohepatic circulation, small percentage of bile acids escaped reabsorption, entering the systemic circulation to bind to several receptors, such as farnesoid X receptor (FXR), thereby exert their biological effects. Gut microbiota interplays with bile acids by affecting their synthesis and determining the production of secondary bile acids. Reciprocally, bile acids shape out the structure of gut microbiota. The interplay of bile acids and FXR is involved in the development of multisystemic conditions, encompassing metabolic diseases, hepatobiliary diseases, immune associated disorders. In the review, we aim to provide a thorough review of the intricate crosstalk between the gut microbiota and bile acids, the physiological roles of bile acids and FXR in mammals’ health and disease, and the clinical translational considerations of gut microbiota-bile acids-FXR in the treatment of the diseases.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127865"},"PeriodicalIF":6.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kobresia humilis via root-released flavonoids recruit Bacillus for promoted growth","authors":"Junxi Li ,&nbsp;Lingling Wu ,&nbsp;Yizhi Zhou ,&nbsp;Yongli Xie ,&nbsp;Fuwei Lu ,&nbsp;Feifei Chang ,&nbsp;Xue Yang ,&nbsp;Xianzhong Han ,&nbsp;Mingxuan Cheng","doi":"10.1016/j.micres.2024.127866","DOIUrl":"10.1016/j.micres.2024.127866","url":null,"abstract":"<div><p>Alpine meadows, which are critical for biodiversity and ecosystem services, are increasingly degrading, necessitating effective restoration strategies. This study explored the mechanism by which <em>Kobresia humilis</em>, an alpine meadow-constructive species, modulates the rhizosphere microbiome via root exudates to enhance growth. Field investigations revealed that the plant height of <em>K. humilis</em> in a severely degraded (SD) alpine meadow was significantly higher than that in other <em>K. humilis</em> populations. Consequently, we analysed the differences between this plot and other <em>K. humilis</em> samples with different degrees of degradation to explore the reasons underlying the phenotypic differences in <em>K. humilis</em>. 16 S rRNA amplicon sequencing results showed that the SD plots were significantly enriched with more <em>Bacillus</em>, altering the composition of the rhizosphere microbial community of <em>K. humilis</em>. The collection and analysis of root exudates from various <em>K. humilis</em> locations revealed distinct differences. Procrustes analysis indicated a strong correlation between the root exudates and the rhizosphere microbiome composition of <em>K. humilis</em>. Model-based integration of metabolite observations, species abundance 2 (MIMOSA2), and Spearman's rank correlation coefficient analysis were used to identify the root exudates potentially related to the enrichment and recruitment of <em>Bacillus</em>. <em>Bacillus</em> from SD samples was isolated and screened, and the representative strain D334 was found to be differentially enriched compared to other samples. A series of in vitro experiments with the screened root exudates and strain D334 demonstrated that <em>K. humilis</em> could recruit <em>Bacillus</em> and promote its colonisation by releasing flavonoids, particularly baicalin. Additionally, <em>K. humilis</em> can release sucrose and riboflavin, which promote strain growth. Finally, soil microbiome transplantation experiments confirmed that different <em>K. humilis</em> phenotypes were closely related to the functions of the rhizosphere microbiome, especially in root morphological shaping. Moreover, the effects of <em>Bacillus</em> inoculation and the microbiome on the plant phenotypes were consistent. In summary, this study revealed a new mechanism by which <em>K. humilis</em> recruits rhizosphere growth-promoting bacteria and enhances soil nutrient utilisation, thereby promoting plant growth. These findings provide a theoretical basis for ecological restoration using soil microbial communities and clarify the relationship between plant metabolites and microbial community assembly.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127866"},"PeriodicalIF":6.1,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptability of the gut microbiota of the German cockroach Blattella germanica to a periodic antibiotic treatment","authors":"Jesús Marín-Miret ,&nbsp;Ana Elena Pérez-Cobas ,&nbsp;Rebeca Domínguez-Santos ,&nbsp;Benjamí Pérez-Rocher ,&nbsp;Amparo Latorre ,&nbsp;Andrés Moya","doi":"10.1016/j.micres.2024.127863","DOIUrl":"10.1016/j.micres.2024.127863","url":null,"abstract":"<div><p>High-throughput sequencing studies have shown that diet or antimicrobial treatments impact animal gut microbiota equilibrium. However, properties related to the gut microbial ecosystem stability, such as resilience, resistance, or functional redundancy, must be better understood. To shed light on these ecological processes, we combined advanced statistical methods with 16 S rRNA gene sequencing, functional prediction, and fitness analyses in the gut microbiota of the cockroach <em>Blattella germanica</em> subject to three periodic pulses of the antibiotic (AB) kanamycin (n=512). We first confirmed that AB did not significantly affect cockroaches' biological fitness, and gut microbiota changes were not caused by insect physiology alterations. The sex variable was examined for the first time in this species, and no statistical differences in the gut microbiota diversity or composition were found. The comparison of the gut microbiota dynamics in control and treated populations revealed that (1) AB treatment decreases diversity and completely disrupts the co-occurrence networks between bacteria, significantly altering the gut community structure. (2) Although AB also affected the genetic composition, functional redundancy would explain a smaller effect on the functional potential than on the taxonomic composition. (3) As predicted by Taylor's law, AB generally affected the most abundant taxa to a lesser extent than the less abundant taxa. (4) Taxa follow different trends in response to ABs, highlighting \"resistant taxa,\" which could be critical for community restoration. (5) The gut microbiota recovered faster after the three AB pulses, suggesting that gut microbiota adapts to repeated treatments.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127863"},"PeriodicalIF":6.1,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002647/pdfft?md5=52a39a5bc48555f022c32d60933a4cf0&pid=1-s2.0-S0944501324002647-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the secondary metabolome and biocontrol potential of the recently described species Bacillus nakamurai","authors":"François Nimbeshaho ,&nbsp;Gaspard Nihorimbere ,&nbsp;Anthony Argüelles Arias ,&nbsp;Charlotte Liénard ,&nbsp;Sébastien Steels ,&nbsp;Anaclet Nibasumba ,&nbsp;Venant Nihorimbere ,&nbsp;Anne Legrève ,&nbsp;Marc Ongena","doi":"10.1016/j.micres.2024.127841","DOIUrl":"10.1016/j.micres.2024.127841","url":null,"abstract":"<div><p>In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to the recently described <em>Bacillus nakamurai</em> was selected for its strong <em>in vitro</em> antimicrobial activities against a range of bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that BDI-IS1 produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin and bacilysin, together with some some ribosomally-synthesized and post-translationally modified peptides (RiPPs) such as plantazolicin, and potentially amylocyclicin, bacinapeptin and LCI. Reverse genetics further showed the specific involvement of some of these compounds in the antagonistic activity of the strain. Comparative genomics between the five already sequenced <em>B. nakamurai</em> strains showed that non-ribosomal products constitute the core metabolome of the species while RiPPs are more strain-specific. Although the secondary metabolome lacks some key bioactive metabolites found in <em>B. velezensis</em>, greenhouse experiments show that <em>B. nakamurai</em> BDI-IS1 is able to protect tomato and maize plants against early blight and northern leaf blight caused by <em>Alternaria solani</em> and <em>Exserohilum turcicum</em>, respectively, at levels similar to or better than <em>B. velezensis</em> QST713. The reduction of these foliar diseases, following root or leaf application of the bacterial suspension demonstrates that BDI-IS1 can act by direct antibiosis and by inducing plant defence mechanisms. These findings indicate that <em>B. nakamurai</em> BDI-IS1 can be considered as a good candidate for biocontrol of plant diseases prevailing in tropical regions, and encourage further research into its spectrum of activity, its requirements and the conditions needed to ensure its efficacy.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"288 ","pages":"Article 127841"},"PeriodicalIF":6.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002428/pdfft?md5=fffc26b9c220475bd011d61204b1e754&pid=1-s2.0-S0944501324002428-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141993244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic microbial diversity associated with commercial cultivar and crop wild relative banana variety could provide clues for microbial community management","authors":"Luisa F. Posada ,&nbsp;Luis A. Arteaga-Figueroa ,&nbsp;Isabel Adarve-Rengifo ,&nbsp;Maria Cadavid ,&nbsp;Sebastián Zapata ,&nbsp;Javier C. Álvarez","doi":"10.1016/j.micres.2024.127862","DOIUrl":"10.1016/j.micres.2024.127862","url":null,"abstract":"<div><p>Endophytes, microorganisms inhabiting internal plant tissues, play a pivotal role in plant growth and disease resistance. Moreover, previous studies have established that <em>Musa</em> plants derive disease protective functions from their microbiome. Notably, one of the crop wild relatives of banana, the Calcutta 4 variety, exhibits resistance to various phytopathogens such as <em>Pseudocercospora fijiensis</em> (<em>P. fijiensis</em>), while the Williams commercial cultivar (cv.) is highly susceptible. Therefore, this study aims primarily to characterize and compare the endophytic microbiota composition of Calcutta 4 and Williams banana plants when grown sympatrically. Alongside, differences in endophytic microbiome between plant sections (shoot or roots), growth phases (<em>in vitro</em> or greenhouse) and fitness factors such as the addition of plant growth-promoting bacteria <em>Bacillus subtilis</em> EA-CB0575 (T2 treatment) or infection by <em>P. fijiensis</em> (T3 treatment) were examined. Both culture-dependent and -independent techniques were used to evaluate these differences and assess the culturability of banana endophytes under varying conditions. Microbial cultures resulted in 331 isolates distributed across 54 genera when all treatments were evaluated, whereas <em>16 S</em> sequencing produced 9510 ASVs assigned in 1456 genera. Alpha and beta diversity exhibited significant differences based on plant section, with an increase in phylogenetic diversity observed in plants with pathogen infection (T3) compared to control plants (T1). Additionally, four differentially abundant genera associated with nitrogen metabolism were identified in T3 plants and seven genera showed differential abundance when comparing varieties. When culture-dependent and -independent methods were compared, it was found that isolates represented 3.7 % of the genera detected by culture-independent methods, accounting for 12–41 % of the total data depending on the treatment. These results are crucial for proposing management strategies derived from crop wild relatives to enhance the resilience of susceptible commercial varieties against fitness factors affecting crop development. Additionally, they help to decipher the pathogenic effects of <em>P. fijiensis</em> in banana plants and advance the understanding of how plant domestication influences the endosphere.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127862"},"PeriodicalIF":6.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002635/pdfft?md5=eb55da9021cbfb39c09200c035c60b1e&pid=1-s2.0-S0944501324002635-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}