Microbiological research最新文献

{"title":"Microalgae growth-promoting bacteria for cultivation strategies: Recent updates and progress","authors":"Wasu Pathom-aree ,&nbsp;Pachara Sattayawat ,&nbsp;Sahutchai Inwongwan ,&nbsp;Benjamas Cheirsilp ,&nbsp;Naruepon Liewtrakula ,&nbsp;Wageeporn Maneechote ,&nbsp;Pharada Rangseekaew ,&nbsp;Fiaz Ahmad ,&nbsp;Muhammad Aamer Mehmood ,&nbsp;Fengzheng Gao ,&nbsp;Sirasit Srinuanpan","doi":"10.1016/j.micres.2024.127813","DOIUrl":"10.1016/j.micres.2024.127813","url":null,"abstract":"<div><p>Microalgae growth-promoting bacteria (MGPB), both actinobacteria and non-actinobacteria, have received considerable attention recently because of their potential to develop microalgae-bacteria co-culture strategies for improved efficiency and sustainability of the water-energy-environment nexus. Owing to their diverse metabolic pathways and ability to adapt to diverse conditions, microalgal-MGPB co-cultures could be promising biological systems under uncertain environmental and nutrient conditions. This review proposes the recent updates and progress on MGPB for microalgae cultivation through co-culture strategies. Firstly, potential MGPB strains for microalgae cultivation are introduced. Following, microalgal-MGPB interaction mechanisms and applications of their co-cultures for biomass production and wastewater treatment are reviewed. Moreover, state-of-the-art studies on synthetic biology and metabolic network analysis, along with the challenges and prospects of opting these approaches for microalgal-MGPB co-cultures are presented. It is anticipated that these strategies may significantly improve the sustainability of microalgal-MGPB co-cultures for wastewater treatment, biomass valorization, and bioproducts synthesis in a circular bioeconomy paradigm.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insoluble polysaccharides produced in plant cell cultures protect from Clostridioides difficile colitis","authors":"Giulia Bernabè ,&nbsp;Ignazio Castagliuolo ,&nbsp;Andrea Porzionato ,&nbsp;Gino Casarotto ,&nbsp;Renzo Dal Monte ,&nbsp;Andrea Carpi ,&nbsp;Paola Brun","doi":"10.1016/j.micres.2024.127812","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127812","url":null,"abstract":"<div><p><em>Clostridioides difficile</em> infection (CDI) poses a significant health threat due to high recurrence rates. Antimicrobial agents are commonly used to manage CDI-related diarrhoea; however, by aggravating intestinal dysbiosis, antibiotics enable <em>C. difficile</em> spores germination and production of toxins, the main virulence factors. Therefore, the binding of exotoxins using adsorbents represents an attractive alternative medication for the prevention and treatment of relapses. In this study, we provided evidence that the natural insoluble polysaccharides, named ABR119, extracted by plant cell cultures, effectively trap <em>C. difficile</em> toxins. In our experiments, ABR119 exhibited no cytotoxicity <em>in vitro</em> and was safely administered <em>in vivo</em>. In the animal model of <em>C. difficile</em>-associated colitis, ABR119 (50 mg/kg body weight) significantly reduced the colonic myeloperoxidase activity and severity of inflammation, preventing body weight loss. These effects were not evident when we treated animals with wheat bran polysaccharides. We did not detect bacterial killing effects of ABR119 against <em>C. difficile</em> nor against bacterial species of the normal gut microbiota. Moreover, ABR119 did not interfere <em>in vitro</em> with the antimicrobial activities of most clinically used antibiotics. In summary, ABR119 holds promise for treating and preventing <em>C. difficile</em> colitis by trapping the bacterial toxins, warranting further studies to assess the ABR119 potential in human infections caused by <em>C. difficile</em>.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002131/pdfft?md5=10e2ef83a976f2dcf3bbfa710ea060c6&pid=1-s2.0-S0944501324002131-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141483207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclic diguanylate differentially regulates the expression of virulence factors and pathogenesis-related phenotypes in Clostridioides difficile","authors":"Zirou Ouyang,&nbsp;Min Zhao,&nbsp;Jiayiren Li,&nbsp;Yulian Zhang,&nbsp;Jianhong Zhao","doi":"10.1016/j.micres.2024.127811","DOIUrl":"10.1016/j.micres.2024.127811","url":null,"abstract":"<div><p><em>Clostridioides difficile</em> infection (CDI) caused by toxigenic <em>C. difficile</em> is the leading cause of antimicrobial and healthcare-associated diarrhea. The pathogenicity of <em>C. difficile</em> relies on the synergistic effect of multiple virulence factors, including spores, flagella, type IV pili (T4P), toxins, and biofilm. Spores enable survival and transmission of <em>C. difficile</em>, while adhesion factors such as flagella and T4P allow <em>C. difficile</em> to colonize and persist in the host intestine. Subsequently, <em>C. difficile</em> produces the toxins TcdA and TcdB, causing pseudomembranous colitis and other <em>C. difficile</em>-associated diseases; adhesion factors bind to the extracellular matrix to form biofilm, allowing <em>C. difficile</em> to evade drug and immune system attack and cause recurrent infection. Cyclic diguanylate (c-di-GMP) is a near-ubiquitous second messenger that extensively regulates morphology, the expression of virulence factors, and multiple physiological processes in <em>C. difficile.</em> In this review, we summarize current knowledge of how c-di-GMP differentially regulates the expression of virulence factors and pathogenesis-related phenotypes in <em>C. difficile</em>. We highlight that <em>C. difficile</em> spore formation and expression of toxin and flagella genes are inhibited at high intracellular levels of c-di-GMP, while T4P biosynthesis, cell aggregation, and biofilm formation are induced. Recent studies have enhanced our understanding of the c-di-GMP signaling networks in <em>C. difficile</em> and provided insights for the development of c-di-GMP-dependent strategies against CDI.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ancient and remote quartzite caves as a novel source of culturable microbes with biotechnological potential","authors":"Daniele Ghezzi ,&nbsp;Luca Salvi ,&nbsp;Paolo E. Costantini ,&nbsp;Andrea Firrincieli ,&nbsp;Marianna Iorio ,&nbsp;Ettore Lopo ,&nbsp;Margherita Sosio ,&nbsp;Ahmed H. Elbanna ,&nbsp;Zeinab G. Khalil ,&nbsp;Robert J. Capon ,&nbsp;Jo De Waele ,&nbsp;Freddy Vergara ,&nbsp;Francesco Sauro ,&nbsp;Martina Cappelletti","doi":"10.1016/j.micres.2024.127793","DOIUrl":"10.1016/j.micres.2024.127793","url":null,"abstract":"<div><p>Quartzite caves located on table-top mountains (tepuis) in the Guyana Shield, are ancient, remote, and pristine subterranean environments where microbes have evolved peculiar metabolic strategies to thrive in silica-rich, slightly acidic and oligotrophic conditions. In this study, we explored the culturable fraction of the microbiota inhabiting the (ortho)quartzite cave systems in Venezuelan tepui (remote table-top mountains) and we investigated their metabolic and enzymatic activities in relation with silica solubilization and extracellular hydrolytic activities as well as the capacity to produce antimicrobial compounds. Eighty microbial strains were isolated with a range of different enzymatic capabilities. More than half of the isolated strains performed at least three enzymatic activities and four bacterial strains displayed antimicrobial activities. The antimicrobial producers <em>Paraburkholderia bryophila</em> CMB_CA002 and <em>Sphingomonas</em> sp. MEM_CA187, were further analyzed by conducting chemotaxonomy, phylogenomics, and phenomics. While the isolate MEM_CA187 represents a novel species of the genus <em>Sphingomonas</em>, for which the name <em>Sphingomonas imawarii</em> sp. nov. is proposed, <em>P. bryophila</em> CMB_CA002 is affiliated with a few strains of the same species that are antimicrobial producers. Chemical analyses demonstrated that CMB_CA002 produces ditropolonyl sulfide that has a broad range of activity and a possibly novel siderophore. Although the antimicrobial compounds produced by MEM_CA187 could not be identified through HPLC-MS analysis due to the absence of reference compounds, it represents the first soil-associated <em>Sphingomonas</em> strain with the capacity to produce antimicrobials. This work provides first insights into the metabolic potential present in quartzite cave systems pointing out that these environments are a novel and still understudied source of microbial strains with biotechnological potential.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141406582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PcAvh87, a virulence essential RxLR effector of Phytophthora cinnamomi suppresses host defense and induces cell death in plant nucleus","authors":"Haibin He ,&nbsp;Tingyan Xu ,&nbsp;Fuliang Cao ,&nbsp;Yue Xu ,&nbsp;Tingting Dai ,&nbsp;Tingli Liu","doi":"10.1016/j.micres.2024.127789","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127789","url":null,"abstract":"<div><p>Plants have developed intricate immune mechanisms to impede <em>Phytophthora</em> colonization. In response, <em>Phytophthora</em> secretes RxLR effector proteins that disrupt plant defense and promote infection. The specific molecular interactions through which <em>Phytophthora</em> RxLR effectors undermine plant immunity, however, remain inadequately defined. In this study, we delineate the role of the nuclear-localized RxLR effector <em>PcAvh87</em>, which is pivotal for the full virulence of <em>Phytophthora cinnamomi</em>. Gene expression analysis indicates that <em>PcAvh87</em> expression is significantly upregulated during the initial infection stages, interacting with the immune responses triggered by the elicitin protein INF1 and pro-apoptotic protein BAX. Utilizing PEG/CaCl₂-mediated protoplast transformation and CRISPR/Cas9-mediated gene editing, we generated <em>PcAvh87</em> knockout mutants, which demonstrated compromised hyphal growth, sporangium development, and zoospore release, along with a marked reduction in pathogenicity. This underscores PcAvh87’s crucial role as a virulence determinant. Notably, PcAvh87, conserved across the <em>Phytophthora</em> genus, was found to modulate the activity of plant immune protein 113, thereby attenuating plant immune responses. This implies that the PcAvh87-mediated regulatory mechanism could be a common strategy in <em>Phytophthora</em> species to manipulate plant immunity. Our findings highlight the multifaceted roles of PcAvh87 in promoting <em>P. cinnamomi</em> infection, including its involvement in sporangia production, mycelial growth, and the targeting of plant immune proteins to enhance pathogen virulence.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324001903/pdfft?md5=bcfe82c7aae28ec2366e03dd1904aec9&pid=1-s2.0-S0944501324001903-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141308395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nontoxigenic Bacteroides fragilis: A double-edged sword","authors":"Yumei Huang,&nbsp;Jiali Cao,&nbsp;Mengpei Zhu,&nbsp;Ziwen Wang,&nbsp;Ze Jin,&nbsp;Zhifan Xiong","doi":"10.1016/j.micres.2024.127796","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127796","url":null,"abstract":"<div><p>The contribution of commensal microbes to human health and disease is unknown. <em>Bacteroides fragilis</em> (<em>B. fragilis</em>) is an opportunistic pathogen and a common colonizer of the human gut. Nontoxigenic <em>B. fragilis</em> (NTBF) and enterotoxigenic <em>B. fragilis</em> (ETBF) are two kinds of <em>B. fragilis</em>. NTBF has been shown to affect the host immune system and interact with gut microbes and pathogenic microbes. Previous studies indicated that certain strains of <em>B. fragilis</em> have the potential to serve as probiotics, based on their observed relationship with the immune system. However, several recent studies have shown detrimental effects on the host when beneficial gut bacteria are found in the digestive system or elsewhere. In some pathological conditions, NTBF may have adverse reactions. This paper presents a comprehensive analysis of NTBF ecology from the host-microbe perspective, encompassing molecular disease mechanisms analysis, bacteria-bacteria interaction, bacteria-host interaction, and the intricate ecological context of the gut. Our review provides much-needed insights into the precise application of NTBF.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141308396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uniqueness of Candida auris cell wall in morphogenesis, virulence, resistance, and immune evasion","authors":"Salam Dakalbab ,&nbsp;Rania Hamdy ,&nbsp;Patrícia Holigová ,&nbsp;Eman J. Abuzaid ,&nbsp;Ameera Abu-Qiyas ,&nbsp;Yasmina Lashine ,&nbsp;Mohammad.G. Mohammad ,&nbsp;Sameh S.M. Soliman","doi":"10.1016/j.micres.2024.127797","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127797","url":null,"abstract":"<div><p><em>Candida auris</em> has drawn global attention due to its alarming multidrug resistance and the emergence of pan resistant strains. <em>C. auris</em> poses a significant risk in nosocomial candidemia especially among immunocompromised patients<em>. C. auris</em> showed unique virulence characteristics associated with cell wall including cell polymorphism, adaptation, endurance on inanimate surfaces, tolerance to external conditions, and immune evasion. Notably, it possesses a distinctive cell wall composition, with an outer mannan layer shielding the inner 1,3-β glucan from immune recognition, thereby enabling immune evasion and drug resistance. This review aimed to comprehend the association between unique characteristics of <em>C. auris</em>’s cell wall and virulence, resistance mechanisms, and immune evasion. This is particularly relevant since the fungal cell wall has no human homology, providing a potential therapeutic target. Understanding the complex interactions between the cell wall and the host immune system is essential for devising effective treatment strategies, such as the use of repurposed medications, novel therapeutic agents, and immunotherapy like monoclonal antibodies. This therapeutic targeting strategy of <em>C. auris</em> holds promise for effective eradication of this resilient pathogen.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141286274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential nested patterns of Anaplasma marginale and Coxiella-like endosymbiont across Rhipicephalus microplus ontogeny","authors":"Lianet Abuin-Denis ,&nbsp;Elianne Piloto-Sardiñas ,&nbsp;Apolline Maitre ,&nbsp;Alejandra Wu-Chuang ,&nbsp;Lourdes Mateos-Hernández ,&nbsp;Patrícia Gonzaga Paulino ,&nbsp;Yamil Bello ,&nbsp;Frank Ledesma Bravo ,&nbsp;Anays Alvarez Gutierrez ,&nbsp;Rafmary Rodríguez Fernández ,&nbsp;Alier Fuentes Castillo ,&nbsp;Luis Méndez Mellor ,&nbsp;Angélique Foucault-Simonin ,&nbsp;Dasiel Obregon ,&nbsp;Mario Pablo Estrada-García ,&nbsp;Alina Rodríguez-Mallon ,&nbsp;Alejandro Cabezas-Cruz","doi":"10.1016/j.micres.2024.127790","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127790","url":null,"abstract":"<div><p>Understanding the intricate ecological interactions within the microbiome of arthropod vectors is crucial for elucidating disease transmission dynamics and developing effective control strategies. In this study, we investigated the ecological roles of <em>Coxiella</em>-like endosymbiont (CLE) and <em>Anaplasma marginale</em> across larval, nymphal, and adult stages of <em>Rhipicephalus microplus</em>. We hypothesized that CLE would show a stable, nested pattern reflecting co-evolution with the tick host, while <em>A. marginale</em> would exhibit a more dynamic, non-nested pattern influenced by environmental factors and host immune responses. Our findings revealed a stable, nested pattern characteristic of co-evolutionary mutualism for CLE, occurring in all developmental stages of the tick. Conversely, <em>A. marginale</em> exhibited variable occurrence but exerted significant influence on microbial community structure, challenging our initial hypotheses of its non-nested dynamics. Furthermore, <em>in silico</em> removal of both microbes from the co-occurrence networks altered network topology, underscoring their central roles in the <em>R. microplus</em> microbiome. Notably, competitive interactions between CLE and <em>A. marginale</em> were observed in nymphal network, potentially reflecting the impact of CLE on the pathogen transstadial-transmission. These findings shed light on the complex ecological dynamics within tick microbiomes and have implications for disease management strategies.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324001915/pdfft?md5=0df061daed6580529184e971393323e4&pid=1-s2.0-S0944501324001915-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141286272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic patterns of carbohydrate metabolism genes in bacterioplankton during marine algal blooms","authors":"Xinyang Li ,&nbsp;Xueyu Cheng ,&nbsp;Junjie Xu ,&nbsp;Jiajun Wu ,&nbsp;Leo Lai Chan ,&nbsp;Zhonghua Cai ,&nbsp;Jin Zhou","doi":"10.1016/j.micres.2024.127785","DOIUrl":"10.1016/j.micres.2024.127785","url":null,"abstract":"<div><p>Carbohydrates play a pivotal role in nutrient recycling and regulation of algal–bacterial interactions. Despite their ecological significance, the intricate molecular mechanisms governing regulation of phycosphere carbohydrates by bacterial taxa linked with natural algal bloom have yet to be fully elucidated. Here, a comprehensive temporal metagenomic analysis was conducted to explore the carbohydrate-active enzyme (CAZyme) genes in two discrete algal bloom microorganisms (<em>Gymnodinium catenatum</em> and <em>Phaeocystis globosa</em>) across three distinct bloom stages: pre-bloom, peak bloom, and post-bloom. Elevated levels of extracellular carbohydrates, primarily rhamnose, galactose, glucose, and arabinose, were observed during the initial and post-peak stages. The prominent CAZyme families identified—glycoside hydrolases (GH) and carbohydrate-binding modules (CBMs)—were present in both algal bloom occurrences. In the <em>G. catenatum</em> bloom, GH23/24 and CBM13/14 were prevalent during the pre-bloom and peak bloom stages, whereas GH2/3/30 and CBM12/24 exhibited increased prevalence during the post-bloom phase. In contrast, the <em>P. globosa</em> bloom had a dominance of GH13/23 and CBM19 in the initial phase, and this was succeeded by GH3/19/24/30 and CBM54 in the later stages. This gene pool variation—observed distinctly in specific genera—highlighted the dynamic structural shifts in functional resources driven by temporal alterations in available substrates. Additionally, ecological linkage analysis underscored a correlation between carbohydrates (or their related genes) and phycospheric bacteria, hinting at a pattern of bottom-up control. These findings contribute to understanding of the dynamic nature of CAZymes, emphasizing the substantial influence of substrate availability on the metabolic capabilities of algal symbiotic bacteria, especially in terms of carbohydrates.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141274806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct fungal communities affecting opposite galanthamine accumulation patterns in two Lycoris species","authors":"Ziying Wang ,&nbsp;Jie Yuan ,&nbsp;Ren Wang ,&nbsp;Sheng Xu ,&nbsp;Jiayu Zhou","doi":"10.1016/j.micres.2024.127791","DOIUrl":"10.1016/j.micres.2024.127791","url":null,"abstract":"<div><p><em>Lycoris radiata</em> is the main source of galanthamine, a clinical drug used in Alzheimer’s disease; however, the galanthamine content in <em>L. radiata</em> is low. <em>Lycoris aurea</em> is another <em>Lycoris</em> species with high galanthamine content. Fungal endophytes can enhance plant secondary metabolite accumulation; thus, we compared the fungal communities in these two <em>Lycoris</em> species to identify certain fungal taxa in <em>L. aurea</em> capable of enhancing galanthamine accumulation. Several fungal endophytes, which were enriched in, exclusively isolated from <em>L. aurea</em>, or showed significant correlations with galanthamine, were demonstrated to enhance the accumulation of only galanthamine but no other Amaryllidaceae alkaloids (AAs) in <em>L. radiata</em>. These fungal endophytes mainly upregulated the downstream genes in the biosynthesis pathways of AAs in <em>L. radiata</em>, suggesting that they may allocate more precursors for galanthamine biosynthesis. This study demonstrated that fungal endophytes from <em>L. aurea</em> with higher galanthamine content can specifically enhance the accumulation of this medicinal alkaloid in other <em>Lycoris</em> species, thereby increasing the galanthamine source and reducing galanthamine separation and purification costs. This study broadens our understanding of the complex interactions between plant secondary metabolites and fungal endophytes.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":null,"pages":null},"PeriodicalIF":6.7,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141278214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}