Microbiological research最新文献

{"title":"Transcriptomic analysis of sub-MIC Eugenol exposition on antibiotic resistance profile in Multidrug Resistant Enterococcus faecalis E9.8","authors":"Natacha Caballero Gómez ,&nbsp;Julia Manetsberger ,&nbsp;Sonia Castillo-Gutiérrez ,&nbsp;Charles W. Knapp ,&nbsp;Nabil Benomar ,&nbsp;Hikmate Abriouel","doi":"10.1016/j.micres.2025.128057","DOIUrl":"10.1016/j.micres.2025.128057","url":null,"abstract":"<div><div>The spread of multidrug-resistant (MDR) bacteria and their resistance genes along the food chain and the environment has become a global threat aggravated by incorrect disinfection strategies. This study analysed the effect of induction by sub-inhibitory concentrations of eugenol – a major ingredient in clove essential oil commonly used in disinfectant agents – on the phenotypic and genotypic response of MDR <em>Enterococcus faecalis</em> E9.8 strain, selected based on the phenotypic response of other enterococci. Eugenol treatment irreversibly reduced several antibiotics’ minimum inhibitory concentration (MIC), confirmed by kinetic studies for kanamycin, erythromycin, and tetracycline. Furthermore, transcriptomic analysis indicated the reversion of antibiotic resistance through direct and indirect measures, such as down-regulation of genes coding for proteins involved in antibiotic resistance, toxin resistance and virulence factors. Regarding antibiotic resistance genes (ARGs), ten differentially expressed genes (five down-regulated and five up-regulated genes) were related to the main transporter families, which present key targets in antibiotic resistance reversion. Our study thus highlights the importance of considering indirectly related genes as targets for antibiotic resistance reversion besides ARGs <em>sensu stricto</em>. These results allow us to propose using eugenol as an antibiotic resistance reversing agent to be included in disinfectant solutions as an excellent alternative to limit the spread of MDR bacteria and their ARGs in the food chain and the environment.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128057"},"PeriodicalIF":6.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas aeruginosa T6SS secretes an oxygen-binding hemerythrin to facilitate competitive growth under microaerobic conditions","authors":"Chunhui Luo ,&nbsp;Huawei Gu ,&nbsp;Damin Pan ,&nbsp;Yixin Zhao ,&nbsp;Anqi Zheng ,&nbsp;Hai Zhu ,&nbsp;Chen Zhang ,&nbsp;Chen Li ,&nbsp;Jing Zhang ,&nbsp;Can Chen ,&nbsp;Lei Xu ,&nbsp;Junfeng Pan ,&nbsp;Xihui Shen ,&nbsp;Yao Wang","doi":"10.1016/j.micres.2025.128052","DOIUrl":"10.1016/j.micres.2025.128052","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> is a prominent respiratory pathogen in cystic fibrosis (CF) patients, thriving in the hypoxic airway mucus. Previous studies have established the role of the oxygen-binding hemerythrin, Mhr, in enhancing <em>P. aeruginosa</em>'s fitness under microaerobic conditions. However, the specific mechanisms by which Mhr operates remain unclear. This study uniquely identifies Mhr as an effector of the H2-Type VI Secretion System (H2-T6SS) and elucidates its role in the transport and interaction mechanisms that confer a growth advantage under microaerobic conditions. Our findings demonstrate that <em>mhr</em> expression is directly regulated by Anr and Dnr. Western blot analysis confirms that Mhr is secreted extracellularly via the H2-T6SS. The oxygen-binding Mhr re-enters <em>P. aeruginosa</em> through the OprG porin. Then, Mhr interacts with cbb3-type cytochrome c oxidase (cbb3-CcO) subunits CcoP1/CcoP2, significantly impacting intracellular NADH/NAD⁺ levels. These insights suggest that the T6SS-mediated secretion and transport of Mhr represent a novel mechanism by which <em>P. aeruginosa</em> acquires and delivers oxygen, potentially enhancing microaerobic respiration, energy production, and growth under microaerobic conditions.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128052"},"PeriodicalIF":6.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GlSIRT1 deacetylates and activates pyruvate kinase to improve pyruvate content and enhance heat stress resistance in Ganoderma lucidum","authors":"Jing Han ,&nbsp;Xin Tang ,&nbsp;Lingshuai Wang ,&nbsp;Huhui Chen ,&nbsp;Rui Liu ,&nbsp;Mingwen Zhao","doi":"10.1016/j.micres.2025.128055","DOIUrl":"10.1016/j.micres.2025.128055","url":null,"abstract":"<div><div>Heat stress is a prevalent environmental stressor. Previous studies have shown that heat stress drives many cellular changes in <em>Ganoderma lucidum</em>. Interestingly, glycolysis is activated during heat stress, which could contribute to increased heat resistance. However, the molecular mechanisms underlying the enhanced heat resistance of <em>G. lucidum</em> following heat exposure are not yet fully understood. In this study, we explored the possibility that acetylation modification plays a significant role in responses to abiotic stress. After heat treatment, an enhanced interaction between the deacetylase GlSIRT1 and pyruvate kinase (PK) was observed, and the acetylation level of PK was decreased. Further studies revealed that GlSIRT1 increases PK activity through deacetylation, thereby increasing pyruvate content. Consistent with these findings, both PK activity and pyruvate content were reduced in <em>GlSIRT1</em> knockdown strains, which exhibited greater sensitivity to heat stress compared to the wild-type (WT) strain. Collectively, our results reveal a novel molecular mechanism by which heat treatment increases pyruvate content.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128055"},"PeriodicalIF":6.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Androgen deficiency-induced loss of Lactobacillus salivarius extracellular vesicles is associated with the pathogenesis of osteoporosis","authors":"Wenjun Zhang ,&nbsp;Jian Xie ,&nbsp;Zhuoya Wang ,&nbsp;Yuchun Zhong ,&nbsp;Li Liu ,&nbsp;Jun Liu ,&nbsp;Wenming Zhang ,&nbsp;Yimin Pi ,&nbsp;Furui Tang ,&nbsp;Zehong Liu ,&nbsp;Yinjin Shao ,&nbsp;Tian Liu ,&nbsp;Cihua Zheng ,&nbsp;Jun Luo","doi":"10.1016/j.micres.2025.128047","DOIUrl":"10.1016/j.micres.2025.128047","url":null,"abstract":"<div><div>Male osteoporosis is primarily caused by a decrease in testicular testosterone production. Male osteoporosis remains a disease with insufficient diagnosis and treatment, and its consequences are severe, especially in older patients. The gut microbiota plays a crucial role in its occurrence and development. Our study found that the relative abundance of <em>Lactobacillus salivarius</em> in the fecal microbiota of male patients with osteoporosis was significantly lower than that in healthy volunteers. Animal experiments have shown that orchiectomy (ORX) can induce osteoporosis and disrupt the intestinal mucosal barrier, and intestinal microbiota. In addition, we discovered a potential etiological connection between the decreased abundance of the intestinal bacterium <em>L. salivarius</em> and the occurrence of ORX-induced osteoporosis. Cohousing or direct colonization of the intestinal microbiota from healthy rats or direct oral administration of the bacteria alleviated ORX-induced osteoporosis and repaired the intestinal mucosal barrier. Finally, we demonstrated that the extracellular vesicles (EVs) of <em>L. salivarius</em> could be transported to the bones and mitigate ORX-induced osteoporosis in rats. Our results indicate that the gut microbiota participates in protecting bones by secreting and delivering bacterial EVs, and that the reduction of <em>L. salivarius</em> and its EVs is closely related to the development of androgen deficiency-related osteoporosis.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128047"},"PeriodicalIF":6.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial diversity and interactions: Synergistic effects and potential applications of Pseudomonas and Bacillus consortia","authors":"Lixue Wang,&nbsp;Xinyi Zhang,&nbsp;Jiahui Lu,&nbsp;Lingxia Huang","doi":"10.1016/j.micres.2025.128054","DOIUrl":"10.1016/j.micres.2025.128054","url":null,"abstract":"<div><div>Microbial diversity and interactions in the rhizosphere play a crucial role in plant health and ecosystem functioning. Among the myriads of rhizosphere microbes, <em>Pseudomonas</em> and <em>Bacillus</em> are prominent players known for their multifaceted functionalities and beneficial effects on plant growth. The molecular mechanism of interspecies interactions between natural isolates of <em>Bacillus</em> and <em>Pseudomonas</em> in medium conditions is well understood, but the interaction between the two <em>in vivo</em> remains unclear. This paper focuses on the possible synergies between <em>Pseudomonas</em> and <em>Bacillus</em> associated in practical applications (such as recruiting beneficial microbes, cross-feeding and niche complementarity), and looks forward to the application prospects of the consortium in agriculture, human health and bioremediation. Through in-depth understanding of the interactions between <em>Pseudomonas</em> and <em>Bacillus</em> as well as their application prospects in various fields, this study is expected to provide a new theoretical basis and practical guidance for promoting the research and application of rhizosphere microbes.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128054"},"PeriodicalIF":6.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Akkermansia muciniphila inhibits jejunal lipid absorption and regulates jejunal core bacteria","authors":"Qiming Ma ,&nbsp;Xincheng Zhou ,&nbsp;Weikang Su ,&nbsp;Qingyu Wang ,&nbsp;Guoxing Yu ,&nbsp;Weihua Tao ,&nbsp;Zhiyong Dong ,&nbsp;Cunchuan Wang ,&nbsp;Chi-Ming Wong ,&nbsp;Tiemin Liu ,&nbsp;Shiqi Jia","doi":"10.1016/j.micres.2025.128053","DOIUrl":"10.1016/j.micres.2025.128053","url":null,"abstract":"<div><div>Insufficiency of <em>Akkermansia muciniphila</em> (Akk) has been implicated in the pathogenesis of metabolic diseases, and administration or restoration of Akk has ameliorated these disorders. Recently, Pasteurized Akk (PA-Akk) has been approved as a functional food. However, the impact of Akk on lipid absorption in the proximal intestine, which is directly exposed to orally administered Akk, remains largely unexplored. In this study, we orally administered Akk and PA-Akk to mice and investigated the subsequent lipid absorption. Long-term administration of Akk resulted in reduced lipid deposits in the liver and adipocytes, along with improved glucose metabolism. This was primarily attributed to a reduction in lipid absorption by epithelial cells in the proximal jejunum. Mechanistically, Akk activated AMP-activated protein kinase (AMPK) and directly inhibit lipids absorption in both mouse and human jejunal epithelial cells. Furthermore, we demonstrated that Akk treatment, but not PA-Akk treatment, promotes the abundance of genera that are highly abundant in the normal jejunum and belong to the phylum Firmicutes. Thus, our study concludes that oral administration of Akk provides beneficial effects on metabolism, partially through inhibiting jejunal lipid absorption and promoting the abundance of core jejunal microbes.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128053"},"PeriodicalIF":6.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome succession on the pomegranate phylloplane during bacterial blight dysbiosis: Functional implications for blight suppression","authors":"Vinod Chouhan ,&nbsp;Sunil Thalor ,&nbsp;K. Charishma ,&nbsp;Mohammed Javed ,&nbsp;Shanu Kumar ,&nbsp;Jyotsana Sharma ,&nbsp;Vibuthi Munjal ,&nbsp;Aundy Kumar","doi":"10.1016/j.micres.2025.128050","DOIUrl":"10.1016/j.micres.2025.128050","url":null,"abstract":"<div><div>Bacterial blight of pomegranate caused by <em>Xanthomonas axonopodis</em> pv. <em>punicae</em> poses significant challenges to sustainable cultivation, necessitating eco-friendly management strategies, and this study explores the role of the phylloplane microbiome in disease suppression through metabarcoding, traditional microbiology, and antibacterial screening of microbial candidates. Here, we mapped the phylloplane microbiome of pomegranate cultivar 'Bhagwa' during bacterial blight development using metabarcoding sequencing (2443,834 reads), traditional microbiological methods (nutrient-rich and minimal media), and scanning electron microscopy. We observed shifts in microbial diversity, with <em>Xanthomonas</em> typically released through stomata as the blight progressed from water-soaked early lesion to advanced necrotic lesion. The Shannon diversity index peaked at 2.6 in early necrotic stages but dropped to 2.1 in advanced blight. Proteobacteria and Firmicutes were the dominant phyla, with significant compositional changes between disease stages. <em>Bacillus</em> species were prevalent throughout, peaking in both early and severe lesions. <em>Pantoea</em> and <em>Curtobacterium</em> increased during severe blight, while <em>Exiguobacterium</em> thrived on the abaxial surface. A core microbiome, including <em>Pantoea</em>, <em>Enterobacter</em>, and <em>Pseudomonas</em>, remained consistent across stages. Antibacterial screening of 116 bacterial candidates, dominated by <em>Pantoea</em> (32), <em>Bacillus</em> (18), and <em>Pseudomonas</em> (11), revealed multipronged activities against <em>X. axonopodis</em> pv. <em>punicae</em>. <em>Bacillus amyloliquefaciens</em> P2–1 and <em>Pantoea dispersa</em> Pg-Slp-6 suppressed the pathogen through secreted metabolites, while <em>Pantoea dispersa</em> Pg-Slp-6, <em>Pseudomonas oryzihabitans</em> Pg<em>-</em>Slp-82, and <em>Pantoea dispersa</em> Pg-slp-117 exhibited volatile-mediated suppression. Among these, <em>Bacillus amyloliquefaciens</em> P2–1 and <em>Pantoea dispersa</em> Pg-slp-6 showed 55 % and 42 % blight suppression, respectively, highlighting their potential as biocontrol agents.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128050"},"PeriodicalIF":6.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strain diversity and host specificity of the gut symbiont Gilliamella in Apis mellifera, Apis cerana and Bombus terrestris","authors":"Nihong Zhou ,&nbsp;Qiulan Zheng ,&nbsp;Yao Liu ,&nbsp;Zhichu Huang ,&nbsp;Ye Feng ,&nbsp;Yanping Chen ,&nbsp;Fuliang Hu ,&nbsp;Huoqing Zheng","doi":"10.1016/j.micres.2025.128048","DOIUrl":"10.1016/j.micres.2025.128048","url":null,"abstract":"<div><div>Social bees, with their specialized gut microbiota and societal transmission between individuals, provide an ideal model for studying host-gut microbiota interactions. While the functional disparities arising from strain-level diversity of gut symbionts and their effects on host health have been studied in <em>Apis mellifera</em> and bumblebees, studies focusing on host-specific investigations of individual strains across different honeybee hosts remain relatively unexplored. In this study, the complete genomic sequences of 17 strains of <em>Gilliamella</em> from <em>A. mellifera</em>, <em>Apis cerana</em> and <em>Bombus terrestris</em> were analyzed. The analysis revealed that the strains of <em>A. mellifera</em> display a more expansive genomic and functional content compared to the strains of <em>A. cerana</em> and <em>B. terrestris</em>. Phylogenetic analysis showed a deep divergence among the <em>Gilliamella</em> strains from different hosts. Additionally, biochemistry tests and antibiotic susceptibility tests revealed that gut strains from <em>A. mellifera</em> exhibited a more extensive pathway for carbohydrate metabolism and a greater resistance to antibiotics than gut strains from <em>A. cerana</em> and <em>B. terrestris</em>. Strains from <em>A. mellifera</em> and <em>A. cerana</em> showed higher colonization efficiency and competitive ability whithin their respective host species, indicating a higher degree of host-specific adaptation of local gut microbiota. In addition, colonization by <em>A. mellifera</em>-derived strain triggers a stronger transcriptional response in the host than <em>A. cerana</em>-derived strain. The variation in the number of differentially expressed genes and the involvement of distinct signaling pathways across these two host species suggest species-specific adaptations to <em>Gilliamella</em> strains. These findings suggest that despite occupying similar niches in the bee gut, strain-level variations can influence microbial functions, and their impact on host physiological functions may vary across different strains.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128048"},"PeriodicalIF":6.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of two novel tmexCD-toprJ subtypes mediating tigecycline resistance in the megaplasmids from Pseudomonas putida","authors":"Chengzhen Wang ,&nbsp;Xun Gao ,&nbsp;Xiaoyu Zhang ,&nbsp;Chao Yue ,&nbsp;Luchao Lv ,&nbsp;Litao Lu ,&nbsp;Jian-Hua Liu","doi":"10.1016/j.micres.2025.128051","DOIUrl":"10.1016/j.micres.2025.128051","url":null,"abstract":"<div><div>The widespread antimicrobial resistance (AMR) problem poses a serious health threat, leaving few drug choices, including tigecycline, to treat multidrug resistance pathogens. However, a plasmid-borne tigecycline resistance gene cluster, <em>tmexCD1-toprJ1</em>, emerged and conferred tigecycline resistance. In this study, we identified two novel subtypes, <em>tmexCD2.3-toprJ2.3</em> and <em>tmexCD2.4-toprJ1b</em>, obtained from three chicken-origin <em>Pseudomonas putida</em> isolates. Two types of megaplasmids were found as the vital vehicle of these <em>tmexCD-toprJ</em> variants. Phylogenetic and genomic analysis indicated the two variants were mainly distributed in <em>Pseudomonas</em> and acted as an evolved intermediated state precursor of <em>tmexCD2-toprJ2</em>. Further gene cloning assay revealed both the expression of <em>tmexCD2.3-toprJ2.3</em> and <em>tmexCD2.4-toprJ1b</em> could confer multiple antimicrobial resistance, mediating 8- to 16-fold increase of tigecycline MIC. Importantly, two key nucleotide differences in promoter region influence the promoter activity between P_{<em>tmexC2.3</em>} and P_{<em>tmexC2.4</em>}, while the downregulation effect of TNfxB on the transcriptional expression level of <em>tmexCD2.3-toprJ2.3</em> and <em>tmexCD2.4-toprJ1b</em> were observed. The emergency of two novel <em>tmexCD-toprJ</em> variants necessitates preventive measures to curb their spread and highlights concerns about more emerging <em>tmexCD-toprJ</em> variants.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"Article 128051"},"PeriodicalIF":6.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid emergence, transmission, and evolution of KPC and NDM coproducing carbapenem-resistant Klebsiella pneumoniae","authors":"Jiayang Li ,&nbsp;Wenqi Wu ,&nbsp;Hao Wu ,&nbsp;Jinjian Huang ,&nbsp;Ze Li ,&nbsp;Jiajie Wang ,&nbsp;Zhitao Zhou ,&nbsp;Meilin Wu ,&nbsp;Xiuwen Wu ,&nbsp;Yun Zhao ,&nbsp;Jianan Ren","doi":"10.1016/j.micres.2025.128049","DOIUrl":"10.1016/j.micres.2025.128049","url":null,"abstract":"<div><div>Due to the limited treatment options, the widespread of carbapenem-resistant <em>Klebsiella pneumoniae</em> (CRKP) has become a serious clinical challenge. The emergence of <em>Klebsiella pneumoniae</em> carbapenemase (KPC) and New Delhi metallo-β-lactamase (NDM) coproducing CRKP (KPC-NDM-CRKP) further aggravates this issue. In this study, we identified 15 KPC-2-NDM-5-CRKPs as being responsible for an outbreak that involved 10 patients from October 2020 to May 2021. The outbreak was sustained by ST11-KL47-OL101 KPC-2-NDM-5-CRKPs, which exhibited non-susceptible to all antimicrobials available in mainland China. Of these strains, we characterized a conjugative hybrid plasmid co-harboring <em>bla</em>_KPC-2 and <em>bla</em>_NDM-5 with high stability. Plasmid comparison and phylogenetic analysis were performed to investigate the origin of the hybrid plasmid and its fusion mechanism. It was speculated that the hybrid plasmid might originate from <em>Klebsiella pneumoniae subsp. pneumoniae</em> strain kpn-hnqyy plasmids unnamed1 (encoding NDM-5) and unnamed2 (encoding KPC-2). The fusion of these two plasmids was presumably mediated by IS<em>26</em>. Global genomic surveillance raised an alarm about the increased prevalence of KPC-NDM-CRKPs. Phylogenetic evaluation was carried out with a total of 327 KPC-NDM-CRKP genomes to provide a global perspective on such strains, and potential transmission events in other global regions were also observed during the COVID-19 period. The outbreak of such strains in the real world and the co-transfer of <em>bla</em>_KPC and <em>bla</em>_NDM would exacerbate the dispersal of KPC-NDM-CRKPs, which poses a severe threat to public health.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128049"},"PeriodicalIF":6.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}