Microbiological research最新文献

{"title":"Corrigendum to \"Identification of metabolites produced by six gut commensal Bacteroidales strains using non-targeted LC-MS/MS metabolite profiling\" [Microbiol. Res. 283 (2024) 1-11].","authors":"Maria Victoria Fernandez-Cantos, Ambrin Farizah Babu, Kati Hanhineva, Oscar P Kuipers","doi":"10.1016/j.micres.2024.128023","DOIUrl":"https://doi.org/10.1016/j.micres.2024.128023","url":null,"abstract":"","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":" ","pages":"128023"},"PeriodicalIF":6.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of folate biosynthesis defects in Lactiplantibacillus plantarum.","authors":"Jing-Jing Cao, Zhen Liu, Ben-Tao Xiao, Shu-Hong Li, En Yang, Chen-Jian Liu, Xiao-Ran Li","doi":"10.1016/j.micres.2024.128014","DOIUrl":"https://doi.org/10.1016/j.micres.2024.128014","url":null,"abstract":"<p><p>Folate is an essential nutrient for nearly all organisms. While the physiological function and mechanism aspects of folate have been extensively and deeply investigated in Eukarya, related researches in Bacteria remains poorly understood. In this study, we focus on physiological functions of folate in Lactiplantibacillus plantarum by employing a combination of genetics, biochemistry and microscopy approaches. Deletion of the genes folE, folP, or both folE and folK in the folate biosynthesis pathway generated the mutant strains ΔfolE, ΔfolP, and ΔfolKE, respectively. Folate production in ΔfolE, ΔfolKE, and ΔfolP decreased to 51 %, 32 %, and 74 % of the wild-type level, respectively. Simultaneous deletion folE and folK distinctly extended the glutamate tail of folate. These mutants exhibited severely impaired growth capacity under normal conditions. Notably, only ΔfolP cells precipitated in liquid culture. All mutant strains displayed increased cell length, with the extent of elongation correlating to intracellular folate levels. It is noticed that DNA content was increased along with the cell size in deletion mutants. Additionally, 12 % of ΔfolKE cells and 4 % of ΔfolP cells exhibited abnormal lysis, characterized by granular cytoplasm. These findings provide significant insights into the physiological roles of folate in Bacteria.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"128014"},"PeriodicalIF":6.1,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decomposition solutions from brassica and cereal residues suppress tomato bacterial wilt disease by regulating rhizosphere microbial communities.","authors":"Danmei Gao, Zhenxing Fang, Xinjie Pan, Shouwei Liu, Asad Ullah, Musawar Ibrahim, Xingang Zhou, Ying Zhang, Fengzhi Wu","doi":"10.1016/j.micres.2024.128010","DOIUrl":"https://doi.org/10.1016/j.micres.2024.128010","url":null,"abstract":"<p><p>Cover crops can suppress the following crop diseases and alter soil microbial communities, but the mechanisms of such disease suppressive effects remain uncertain. Here, we studied the effects of brassica and cereal cover crops, along with decomposition solutions from these crop residues, on tomato growth and bacterial wilt. Moreover, tomato rhizosphere microorganisms were analyzed by qPCR and high-throughput sequencing. Rhizosphere transplant experiment was conducted to validate the disease suppressive potential of rhizosphere microorganisms mediated by decomposition solutions from these crop residues. Our findings revealed that brassica and cereal cover crops especially wheat, pakchoi and rape significantly enhanced tomato growth and inhibited bacterial wilt disease. Decomposition solutions from brassica and cereal residues had inhibitory effects on Ralstonia solanacearum and this disease. Moreover, such decomposition solutions can differently alter the abundances, compositions and diversities of tomato rhizosphere bacterial and fungal communities. Notably, decomposition solutions from wheat, pakchoi and rape residues increased the inverse Simpson diversity and the abundances of Bacillus spp. community. In addition, decomposition solutions from wheat and pakchoi residues significantly increased bacterial beta diversity, and decomposition solutions from rape residue significantly increased fungal beta diversity. Rhizosphere transplant experiment confirmed that the rhizosphere microbial changes induced by decomposition solutions contributed to the suppressiveness of tomato bacterial wilt disease. These suppressive effects were stronger in decomposition solutions from wheat, pakchoi and rape residues than those from oilseed rape, wild rocket and Indian mustard residues. Overall, our results demonstrated that decomposition solutions from brassica and cereal residues enhance disease suppression by shaping a beneficial rhizosphere microbiota, providing a promising strategy for sustainable management of bacterial wilt in tomato cultivation.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"128010"},"PeriodicalIF":6.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel Brucella T4SS effector RS15060 acts on bacterial morphology, lipopolysaccharide core synthesis and host proinflammatory responses, which is beneficial for Brucella melitensis virulence.","authors":"Yi Yin, Mingxing Tian, Guangdong Zhang, Chan Ding, Shengqing Yu","doi":"10.1016/j.micres.2024.128015","DOIUrl":"https://doi.org/10.1016/j.micres.2024.128015","url":null,"abstract":"<p><p>Brucella relies on the type IV secretion system (T4SS) to establish replication niches within host cells. However, the Brucella T4SS effectors and their functions have not been fully identified. In this study, we investigated the function of Brucella RS15060, a novel T4SS effector discovered in our previous study, on the bacterial biological characteristics and pathogenesis by construction of the gene deletion and complementation strains. We found that deletion of the rs15060 gene weakened abilities of Brucella to replicate within host cells and establish chronic infection in mice but enhanced abilities to adhere/invade HeLa cells and evade lysosomal degradation in the early stage of infection. In addition, the rs15060 deletion Brucella strain showed significant changes in bacterial shape, cell wall thickness, and sensitivity to bactericidal factors. Furthermore, the rs15060 deletion strain showed an increased synthesis of bacterial lipopolysaccharide core and induced a stronger host's inflammatory response. The Brucella rs15060 complementation strain restored the altered biological characteristics. Moreover, BLASTP prediction and 3D structure simulation revealed that the Brucella RS15060 contains NAD(P)-binding and active motifs in structure, which are important for proteins to exert NAD dependent epimerase/dehydratase activity. The complementation strain with mutation on NAD(P)-binding and/or active motifs of RS15060 did not restore the altered characteristics, suggesting that the Brucella RS15060 is a potential NAD dependent epimerase/dehydratase, and the predicted NAD(P)-binding and/or active motifs play an important role on bacterial cell wall and LPS core synthesis, which is crucial for maintaining bacterial morphology and exerting virulence.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"128015"},"PeriodicalIF":6.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmonella: Infection mechanism and control strategies.","authors":"Juane Lu, Hao Wu, Shengbo Wu, Shengli Wang, Hongfei Fan, Haihua Ruan, Jianjun Qiao, Qinggele Caiyin, Mingzhang Wen","doi":"10.1016/j.micres.2024.128013","DOIUrl":"https://doi.org/10.1016/j.micres.2024.128013","url":null,"abstract":"<p><p>Salmonella is a foodborne pathogen that predominantly resides in the intestinal tract of humans and animals. Infections caused by Salmonella can lead to various illnesses, including gastroenteritis, bacteremia, septicemia, and focal infections, with severe cases potentially resulting in host mortality. The mechanisms by which Salmonella invades host cells and disseminates throughout the body are partly understood, but there are still many scientific questions to be solved. This review aims to synthesize existing research on the interactions between Salmonella and hosts, detailing a comprehensive infection mechanism from adhesion and invasion to intracellular propagation and systemic spread. Overuse of antibiotics contributes to the emergence of drug-resistant Salmonella strains. An in-depth analysis of the mechanism of Salmonella infection will provide a theoretical basis for the development of novel Salmonella control strategies. These innovative control strategies include antibiotic adjuvants, small molecules, phages, attenuated vaccines, and probiotic therapies, which show huge potential in controlling Salmonella infection.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"128013"},"PeriodicalIF":6.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Listeria monocytogenes colonises established multispecies biofilms and resides within them without altering biofilm composition or gene expression.","authors":"Eva M Voglauer, Lauren V Alteio, Nadja Pracser, Sarah Thalguter, Narciso M Quijada, Martin Wagner, Kathrin Rychli","doi":"10.1016/j.micres.2024.127997","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127997","url":null,"abstract":"<p><p>Listeria (L.) monocytogenes can survive for extended periods in the food producing environment. Here, biofilms possibly provide a niche for long-term survival due to their protective nature against environmental fluctuations and disinfectants. This study examined the behaviour of a L. monocytogenes ST121 isolate in a multispecies biofilm composed of Pseudomonas (P.) fragi, Brochothrix (B.) thermosphacta, and Carnobacterium (C.) maltaromaticum, previously isolated from a meat processing facility. The composition of the biofilm community and matrix, and transcriptional activity were analysed. L. monocytogenes colonised the multispecies biofilm, accounting for 6.4 % of all total biofilm cells after six hours. Transcriptomic analysis revealed 127 significantly up-regulated L. monocytogenes genes compared to the inoculum, including motility, chemotaxis, iron, and protein transport related genes. When comparing the differentially expressed transcripts within the multispecies biofilm with and without L. monocytogenes, only a cadmium/zinc exporting ATPase gene in C. maltaromaticum was significantly upregulated, while the other 9313 genes in the biofilm community showed no significant differential expression. We further monitored biofilm development over time (6, 24 hours and 7 days). P. fragi remained the dominant species, while L. monocytogenes was able to survive in the multispecies biofilm accounting for 2.4 % of total biofilm cells after 7 days, without any significant changes in its abundance. The presence of L. monocytogenes did neither alter the biofilm community nor its matrix composition (amount of extracellular DNA, carbohydrates, and protein). Our data indicate that L. monocytogenes resides in multispecies biofilms, potentially increasing survival against cleaning and disinfection in food processing environments, supporting persistence.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"127997"},"PeriodicalIF":6.1,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ratio of reactive oxygen and nitrogen species determines the type of cell death that bacteria undergo.","authors":"Athanasios Nikolaou, Manuel Salvador, Ian Wright, Thomas Wantock, Gavin Sandison, Thomas Harle, Daniela Carta, Jorge Gutierrez-Merino","doi":"10.1016/j.micres.2024.127986","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127986","url":null,"abstract":"<p><p>Reactive oxygen and nitrogen species (RONS) are emerging as a novel antibacterial strategy to combat the alarming increase in antimicrobial resistance (AMR). RONS can inhibit bacterial growth through reactions with cellular molecules, compromising vital biological functions and leading to cell death. While their mechanisms of action have been studied, many remain unclear, especially in biologically relevant environments. In this study, we exposed Gram-positive and Gram-negative bacteria to varying RONS ratios, mimicking what microbes may naturally encounter. A ratio in favour of RNS induced membrane depolarization and pore formation, resulting in an irreversible bactericidal effect. By contrast, ROS predominance caused membrane permeabilization and necrotic-like responses, leading to biofilm formation. Furthermore, bacterial cells exposed to more RNS than ROS activated metabolic processes associated with anaerobic respiration, DNA & cell wall/membrane repair, and cell signalling. Our findings suggest that the combination of ROS and RNS can be an effective alternative to inhibit bacteria, but only under higher RNS levels, as ROS dominance might foster bacterial tolerance, which in the context of AMR could have devastating consequences.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"127986"},"PeriodicalIF":6.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Links between oropharyngeal microbiota and IgA nephropathy: A paradigm shift from isolated microbe to microbiome.","authors":"Narongsak Tangon, Sirinart Kumfu, Nipon Chattipakorn, Siriporn C Chattipakorn","doi":"10.1016/j.micres.2024.128005","DOIUrl":"https://doi.org/10.1016/j.micres.2024.128005","url":null,"abstract":"<p><p>Immunoglobulin A nephropathy (IgAN) is the most prevalent form of primary glomerulonephritis globally, yet its pathogenesis remains incompletely understood. While much research has focused on the gut microbiome in the development of the disease, emerging evidence suggests that the oropharyngeal microbiota may also be a potential contributor. Studies have revealed significant alterations in oropharyngeal microbial diversity and specific bacterial taxa in IgAN patients, correlating with disease severity and progression. This review aims to comprehensively summarize and discuss the key findings from in vitro, in vivo, and clinical studies into the oropharyngeal bacteria and microbiome alterations in IgAN. Clinical studies have identified associations between certain oropharyngeal bacteria, particularly Cnm⁺Streptococcus mutans, Campylobacter rectus, and Porphyromonas gingivalis with IgAN patients and severe clinical outcomes with. In vitro and in vivo studies further establish a causal relationship between IgAN and oropharyngeal bacteria such as Streptococcus and Haemophilus. Microbiome analyses demonstrate dysbiotic patterns in IgAN patients and identify new potential bacterial genera that have yet to be explored experimentally but may potentially contribute to the disease's pathogenesis. Additionally, the use of these bacterial genera as diagnostic and prognostic biomarkers of IgAN has achieved promising performance. Overall, the evidence highlights the strong connection between oropharyngeal bacteria and IgAN through both causal and non-causal associations. Further investigation into these newly identified bacterial genera and integration of multi-omics data are necessary to uncover mechanisms, validate their role in IgAN, and potentially develop novel diagnostic and therapeutic approaches.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"128005"},"PeriodicalIF":6.1,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial effect of sulconazole in combination with glucose/trehalose against carbapenem-resistant hypervirulent Klebsiella pneumoniae persisters.","authors":"Miaomiao Xie, Kaichao Chen, Heng Heng, Edward Wai-Chi Chan, Sheng Chen","doi":"10.1016/j.micres.2024.128006","DOIUrl":"https://doi.org/10.1016/j.micres.2024.128006","url":null,"abstract":"<p><p>The emergence and rapid dissemination of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) pose a serious threat to public health. Antibiotic treatment failure of K. pneumoniae infections has been largely attributed to acquisition of antibiotic resistance and bacterial biofilm caused by the presence of antibiotic persisters. There is an urgent need for novel antimicrobial agents or therapy strategies to manage infections caused by these notorious pathogens. In this study, we screened a collection of compounds that can dissipate bacterial proton motive force (PMF) and intermediate metabolites that can suppress antibiotic tolerance, and identified an antifungal drug sulconazole which can act in combination with glucose or trehalose to exert strong antibacterial effect against starvation-induced CR-hvKP persisters. Investigation of underlying mechanisms showed that sulconazole alone caused dissipation of transmembrane PMF, and sulconazole used in combination with glucose or trehalose could significantly inhibit the efflux activity, reduce NADH and ATP levels, and cause intracellular accumulation of reactive oxygen species (ROS) in CR-hvKP persisters, eventually resulting in bacterial cell death. These findings suggest that the sulconazole and glucose/trehalose combination is highly effective in eradicating multidrug-resistant and hypervirulent K. pneumoniae persisters, and may be used in development of a feasible strategy for treatment of chronic and recurrent K. pneumoniae infections.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"128006"},"PeriodicalIF":6.1,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Underground fires shape the structure of microbial communities and select for thermophilic bacteria through a temperature gradient.","authors":"Aurora Flores-Piña, Eduardo Valencia-Cantero, Gustavo Santoyo","doi":"10.1016/j.micres.2024.127996","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127996","url":null,"abstract":"<p><p>A detailed diversity analysis of the prokaryotic and fungal communities in soil impacted by an underground fire located in the Trans-Mexican volcanic belt, Mexico, is described. Microbial diversity data obtained from soils at different depths and temperatures (27 °C, 42 °C, 50 ºC and 54 ºC) were analyzed, and Firmicutes increased in abundance as the temperature augmented, and Proteobacteria mainly decreased in abundance at high temperatures compared to unaffected soils. The fungal phylum Ascomycota was the most abundant, with no significant changes. A clear reduction in the richness of both prokaryotic and eukaryotic operational taxonomic units (OTUs) was observed in the affected soils. At the genus level, Bacillus species were the most abundant among bacteria, while Aspergillus, Penicillium, and Mortierella were dominant fungal genera at higher temperatures. Interestingly, the physicochemical parameters of the affected soils modified organic matter, which was indirectly correlated with the presence of some microbial taxa. Likewise, we obtained 308 soil bacterial isolates from both control and affected soils. Among these, the taxa from the phyla Actinobacteria and Firmicutes demonstrated the highest thermotolerance in the affected soils. Our findings shed light on the impact of underground fires on the structure of microbial communities, favoring an abundance of thermotolerant microbes.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"127996"},"PeriodicalIF":6.1,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}