Microbiological research最新文献

Adapted evolution towards flagellar loss in Pseudomonas syringae. 丁香假单胞菌失去鞭毛的适应性进化。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-16 DOI: 10.1016/j.micres.2024.127969

Jiarong Wang, Xiaoquan Yu, Hao Yang, Hanzhong Feng, Yujuan Wang, Nannan Zhang, Haining Xia, Jie Li, Lei Xing, Junfeng Wang, Yongxing He

{"title":"Adapted evolution towards flagellar loss in Pseudomonas syringae.","authors":"Jiarong Wang, Xiaoquan Yu, Hao Yang, Hanzhong Feng, Yujuan Wang, Nannan Zhang, Haining Xia, Jie Li, Lei Xing, Junfeng Wang, Yongxing He","doi":"10.1016/j.micres.2024.127969","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127969","url":null,"abstract":"<p><p>The flagellum is a complex molecular nanomachine crucial for cell motility. Its assembly requires coordinated expression of over 50 flagellar genes, regulated by the transcription activator FleQ. Phylogenomic analyses suggest that many non-flagellated bacterial species have evolved from flagellated ancestors by losing specific flagellar components, though the evolutionary mechanisms driving this process remain unclear. In this study, we examined the evolutionary dynamics of Pseudomonas syringae DC3000 under standard laboratory conditions using quantitative proteomics. We observed a notable reduction in flagellar gene expression following prolonged serial passages. Whole-genome sequencing revealed multiple adaptive mutations in fleQ, dksA, and glnE, all of which are associated with flagellar biosynthesis. Furthermore, our findings demonstrate that nonmotile ΔfleQ cells can hitchhike onto wild-type cells, potentially facilitated by increased production of the surfactant syringafactin. Our study suggests that the high metabolic costs associated with flagella biosynthesis, coupled with advantageous hitchhiking properties, contribute to the degenerative evolution of flagella.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"127969"},"PeriodicalIF":6.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of gut microbiota in rheumatoid arthritis: Potential cellular mechanisms regulated by prebiotic, probiotic, and pharmacological interventions 肠道微生物群在类风湿关节炎中的作用：益生菌、益生菌和药物干预调控的潜在细胞机制。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-12 DOI: 10.1016/j.micres.2024.127973

Jiashang Li, Ruoying Fan, Zhe Zhang, Lihui Zhao, Yu Han, Yue Zhu, Jin-ao Duan, Shulan Su

{"title":"Role of gut microbiota in rheumatoid arthritis: Potential cellular mechanisms regulated by prebiotic, probiotic, and pharmacological interventions","authors":"Jiashang Li, Ruoying Fan, Zhe Zhang, Lihui Zhao, Yu Han, Yue Zhu, Jin-ao Duan, Shulan Su","doi":"10.1016/j.micres.2024.127973","DOIUrl":"10.1016/j.micres.2024.127973","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a chronic autoimmune disease that primarily affects joints and multiple organs and systems, which is long-lasting and challenging to cure and significantly impacting patients' quality of life. Alterations in the composition of intestinal flora in both preclinical and confirmed RA patients indicate that intestinal bacteria play a vital role in RA immune function. However, the mechanism by which the intestinal flora is regulated to improve the condition of RA is not fully understood. This paper reviews the methods of regulating gut microbiota and its metabolites through prebiotics, probiotics, and pharmacological interventions, and discusses their effects on RA. Additionally, it explores the potential predictive role of cellular therapy mechanisms of intestinal flora in treating RA. These findings suggest that restoring the ecological balance of intestinal flora and regulating intestinal barrier function may enhance immune system function, thereby improving rheumatoid arthritis. This offers new insights into its treatment.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127973"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effect of Adathoda vasica plant-derived biostimulant and PGPR on Zea mays L. for drought stress management Adathoda vasica 植物源生物刺激剂和 PGPR 对玉米干旱胁迫管理的协同效应。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-09 DOI: 10.1016/j.micres.2024.127968

Abhilasha Mishra, Srishti Kar, Nikita Bisht, Shashank Kumar Mishra, Puneet Singh Chauhan

{"title":"Synergistic effect of Adathoda vasica plant-derived biostimulant and PGPR on Zea mays L. for drought stress management","authors":"Abhilasha Mishra, Srishti Kar, Nikita Bisht, Shashank Kumar Mishra, Puneet Singh Chauhan","doi":"10.1016/j.micres.2024.127968","DOIUrl":"10.1016/j.micres.2024.127968","url":null,"abstract":"<div><div>Drought is a significant abiotic stress that adversely affects the physiological and biochemical processes in crops, posing a considerable challenge to agricultural productivity. The present study explored the efficacy of plant-derived biostimulant (PDB) and plant growth-promoting rhizobacteria (PGPR) strains <em>Pseudomonas putida</em> (RA) and <em>Paenibacillus lentimorbus</em> CHM12) in the management of negative impacts of drought stress in <em>Zea mays</em> (maize). <em>Adathoda vasica</em> leaf extracts (ADLE) emerged as the most potent biostimulant of the seven evaluated medicinal plant extracts. The synergetic effect of ADLE and RA enhances plant vegetative growth (root length, shoot length, fresh weight and dry weight) as well as significantly modulates drought-induced oxidative stress, as indicated by higher chlorophyll content and increased sugar and phenolic levels and reduction of proline level. The expression of defence-related (<em>ZmAPX, ZmSOD, and ZmCAT</em>) and transcription factor (<em>ZmNAC, ZmWRKY, and ZmMYB</em>) genes further supported the beneficial effects of this synergism under drought conditions. Furthermore, metabolite profiling through GC-MS analysis showed significant alterations in metabolites such as glucose, galactose, mannose, hexopyranose, linolenic acid, hexadecenoic acid, and butanedioic acid when PDB and PGPR were applied together. Overall, the findings of the present study affirm that the combined application of plant-derived biostimulant ADLE and plant-beneficial rhizobacteria RA can effectively alleviate the adverse effects of drought on maize, providing an eco-friendly and sustainable solution for improving productivity under stress.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127968"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The LysR-type transcriptional factor PacR controls heterocyst differentiation and C/N metabolism in the cyanobacterium Anabaena PCC 7120. LysR 型转录因子 PacR 控制着蓝藻 Anabaena PCC 7120 的异囊分化和 C/N 代谢。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-09 DOI: 10.1016/j.micres.2024.127970

Gui-Ming Lin, Ju-Yuan Zhang, Zhi-Hui Shao, Chen Yang, Guo-Ping Zhao, Kai-Yao Huang, Cheng-Cai Zhang

{"title":"The LysR-type transcriptional factor PacR controls heterocyst differentiation and C/N metabolism in the cyanobacterium Anabaena PCC 7120.","authors":"Gui-Ming Lin, Ju-Yuan Zhang, Zhi-Hui Shao, Chen Yang, Guo-Ping Zhao, Kai-Yao Huang, Cheng-Cai Zhang","doi":"10.1016/j.micres.2024.127970","DOIUrl":"https://doi.org/10.1016/j.micres.2024.127970","url":null,"abstract":"<p><p>PacR (All3953) has previously been identified as a global transcriptional regulator of carbon assimilation in cyanobacteria. In the facultative diazotrophic and filamentous cyanobacterium Anabaena PCC 7120 (Anabaena), inactivation of pacR has been shown to affect cell growth under various conditions. Nitrogen fixation in Anabaena occurs in heterocysts, cells differentiated semiregularly along the filaments following deprivation of combined nitrogen such as nitrate or ammonium. Here, we created a markerless deletion mutant of pacR. In addition to its growth defects observed under different light and nitrogen conditions, the mutant could form a high frequency of heterocysts, including heterocyst doublets, even in the presence of nitrate. Inactivation of pacR led to the upregulation of ntcA, a global regulator of nitrogen metabolism and heterocyst formation, as well as downregulation of genes involved in nitrate uptake and assimilation. These changes led to N-limited cells in the presence of nitrate. PacR also regulates most of the genes encoding bicarbonate transport systems. The promoter regions of ntcA, and several other genes involved in nitrogen or carbon uptake and assimilation, as well as patS and hetN involved in heterocyst patterning can be directly recognized by PacR in vitro. These findings, along with previously reported ChIP-seq data, establish PacR as a crucial transcriptional regulator for balancing carbon and nitrogen metabolism in cyanobacteria.</p>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"127970"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coagulase-Negative Staphylococci phages panorama: Genomic diversity and in vitro studies for a therapeutic use 凝固酶阴性葡萄球菌噬菌体全景：基因组多样性和用于治疗的体外研究。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-09 DOI: 10.1016/j.micres.2024.127944

Maria Sequeira Lopes , Maria Daniela Silva , Joana Azeredo , Luís D.R. Melo

引用次数: 0

Deciphering intricate plant-virus interactions: Potyvirids orchestrate protein posttranslational modifications to regulate pathogenicity 解密植物与病毒之间错综复杂的相互作用：Potyvirids协调蛋白质翻译后修饰以调节致病性。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-08 DOI: 10.1016/j.micres.2024.127940

Ali kamran , Muhammad Dilshad Hussain , Tahir Farooq , Fangfang Li , Mehran Khan , Xiangyang Li , Sanwei Yang , Xin Xie

{"title":"Deciphering intricate plant-virus interactions: Potyvirids orchestrate protein posttranslational modifications to regulate pathogenicity","authors":"Ali kamran , Muhammad Dilshad Hussain , Tahir Farooq , Fangfang Li , Mehran Khan , Xiangyang Li , Sanwei Yang , Xin Xie","doi":"10.1016/j.micres.2024.127940","DOIUrl":"10.1016/j.micres.2024.127940","url":null,"abstract":"<div><div>In a molecular-arm-race between viruses and their hosts, viruses have evolved to harness their host's post-translational modifications (PTMs) machinery to gain a competitive edge. These modifications are the most reliable target of plant viruses to overcome the host defence for successful infection. Relatively fewer PTMs i.e., phosphorylation, <em>O</em>-GlcNAcylation, Ubiquitination, and SUMOylation have been studied regulating the potyvirus-plant interaction. Therefore, it is worth drawing attention towards the importance and potential of this undermined but key strategy of potyvirids (members of family Potyviridae) to abduct their host defence line, suggesting to review in detail the existing knowledge of these PTMs and highlight the unexplored modifications that might have played their part in establishing successful infection. The current review provides an understanding of how PTMs execute viral replication and infection dynamics during plant-potyvirid interactions. We highlighted that PTMs linked to CP, NIa-pro, NIb, and VPg are important to specify their host, virulence, overcoming host innate immunity, and most importantly disarming the host of RNA silencing tool of nailing any intruder. The limitations and potential improvements in studying undermined PTMs, including acetylation, glycosylation, methylation, and neddylation, as well as challenges and future perspectives of this inevitable process are mechanistically deciphered in the course of plant-virus interactions. This communication opens new avenues for investigating the fundamental mechanisms of virus infection and the development of new antiviral strategies for sustainable disease managements.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127940"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The hex1 gene of Trichoderma simmonsii is involved in stress responses, biocontrol potential and wheat plant growth 西蒙氏毛霉的 hex1 基因参与了胁迫反应、生物控制潜力和小麦植物生长。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-07 DOI: 10.1016/j.micres.2024.127958

Alberto Pedrero-Méndez, María Illescas, Enrique Monte, Rosa Hermosa

{"title":"The hex1 gene of Trichoderma simmonsii is involved in stress responses, biocontrol potential and wheat plant growth","authors":"Alberto Pedrero-Méndez, María Illescas, Enrique Monte, Rosa Hermosa","doi":"10.1016/j.micres.2024.127958","DOIUrl":"10.1016/j.micres.2024.127958","url":null,"abstract":"<div><div>Woronin bodies are unique organelles in Pezizomycotina fungi that allow hyphae compartmentalization and prevent cytoplasmatic bleeding after mechanical injury. Several studies have related the peroxisomal protein HEX1, the major component of Woronin bodies with other biological processes such as hyphal growth, osmotic stress tolerance and pathogenicity. <em>Trichoderma</em> spp. are plant-beneficial multipurpose biological control agents, and proteomic and transcriptomic studies have shown that HEX1 and its corresponding gene are overrepresented when grown in the presence of fungal cell walls and plant polymers. To further investigate the involvement of <em>hex1</em> in <em>Trichoderma</em> biology, we generated <em>hex1</em> deletion transformants using the wheat endophytic strain <em>T. simmonsii</em> T137 as host. Results confirmed that <em>hex1</em> gene is involved in the prevention of cytoplasmatic bleeding, and also has a role in fungal growth and biocontrol potential against phytopathogenic fungi and oomycetes. The involvement of <em>hex1</em> in the fungal response to osmotic and oxidative stresses is conditioned by the type of stress and by the nutrient richness of the culture medium. The <em>hex1</em> deletion also affected the interaction with wheat, but did not affect the plant protective effect of T137 against water stress. Overall, this study shows the implication of HEX1 in a wide range of biological processes necessary for <em>T. simmonsii</em> to deploy its abilities to be used as an agriculturally beneficial fungus.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127958"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The gut core microbial species Bifidobacterium longum: Colonization, mechanisms, and health benefits 肠道核心微生物物种长双歧杆菌：定植、机制和健康益处

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-07 DOI: 10.1016/j.micres.2024.127966

Yue Xiao , Lijuan Huang , Jianxin Zhao , Wei Chen , Wenwei Lu

{"title":"The gut core microbial species Bifidobacterium longum: Colonization, mechanisms, and health benefits","authors":"Yue Xiao , Lijuan Huang , Jianxin Zhao , Wei Chen , Wenwei Lu","doi":"10.1016/j.micres.2024.127966","DOIUrl":"10.1016/j.micres.2024.127966","url":null,"abstract":"<div><div><em>Bifidobacterium longum</em> (<em>B. longum</em>) is a species of the core microbiome in the human gut, whose abundance is closely associated with host age and health status. <em>B. longum</em> has been shown to modulate host gut microecology and have the potential to alleviate various diseases. Comprehensive understanding on the colonization mechanism of <em>B. longum</em> and mechanism of the host-<em>B. longum</em> interactions, can provide us possibility to prevent and treat human diseases through <em>B. longum</em>-directed strategies. In this review, we summarized the gut colonization characteristics of <em>B. longum</em>, discussed the diet factors that have ability/potential to enrich indigenous and/or ingested <em>B. longum</em> strains, and reviewed the intervention mechanisms of <em>B. longum</em> in multiple diseases. The key findings are as follows: First, <em>B. longum</em> has specialized colonization mechanisms, like a wide carbohydrate utilization spectrum that allows it to adapt to the host's diet, species-level conserved genes encoding bile salt hydrolase (BSHs), and appropriate bacterial surface structures. Second, dietary intervention (e.g., anthocyanins) could effectively improve the gut colonization of <em>B. longum</em>, demonstrating the feasibility of diet-tuned strain colonization. Finally, we analyzed the skewed abundance of <em>B. longum</em> in different types of diseases and summarized the main mechanisms by which <em>B. longum</em> alleviates digestive (repairing the intestinal mucosal barrier by stimulating Paneth cell activity), immune (up-regulating the regulatory T cell (Treg) populations and maintaining the balance of Th1/Th2), and neurological diseases (regulating the kynurenine pathway and quinolinic acid levels in the brain through the gut-brain axis).</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127966"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Hsp90 of Candida albicans contributes to the virulence of the pathogen by activating the NF-κB signaling pathway and inducing macrophage pyroptosis 白色念珠菌的胞外 Hsp90 通过激活 NF-κB 信号通路和诱导巨噬细胞热毒症，增强了病原体的毒力。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-05 DOI: 10.1016/j.micres.2024.127964

Ting Fang , Juan Xiong , Xin Huang , Xinyu Fang , Xuqing Shen , Yuanying Jiang , Hui Lu

{"title":"Extracellular Hsp90 of Candida albicans contributes to the virulence of the pathogen by activating the NF-κB signaling pathway and inducing macrophage pyroptosis","authors":"Ting Fang , Juan Xiong , Xin Huang , Xinyu Fang , Xuqing Shen , Yuanying Jiang , Hui Lu","doi":"10.1016/j.micres.2024.127964","DOIUrl":"10.1016/j.micres.2024.127964","url":null,"abstract":"<div><div>Strategies aimed at targeting fungal extracellular heat shock protein 90 (eHsp90) using vaccines and antibodies have demonstrated encouraging potential in the prevention and management of invasive fungal diseases (IFDs). However, the precise underlying mechanism by which eHsp90 contributes to the heightened virulence of <em>Candida albicans</em> (<em>C. albicans</em>) remains an enigma, awaiting further elucidation. In our current research, we have found that the 47-kDa fragment of <em>C. albicans</em> Hsp90 (CaHsp90), which serves as the primary antigenic determinant, is not degraded within <em>C. albicans</em> cells. Moreover, we have discovered that extracellular CaHsp90 (eCaHsp90) is derived from the components of lysed <em>C. albicans</em> cells. We also generated recombinant CaHsp90 in <em>Escherichia coli</em>, and found that eCaHsp90 spreads beyond the initial <em>C. albicans</em> colonization site, thereby enhancing the overall virulence of the organism. Our results further clarify that eCaHsp90 activates the nuclear factor kappa-B (NF-κB) signaling pathway and upregulates the expression of NACHT, LRR, and PYD domains-containing protein 3 (NLRP3). This upregulation results in the activation of Gasdermin D (GSDMD) and subsequent macrophage pyroptosis, ultimately increasing the virulence of <em>C. albicans</em>. This study provides valuable insights into the mechanism by which eCaHsp90 contributes to the virulence of <em>C. albicans</em>, offering a pharmacological basis for antifungal strategies targeting fungal eHsp90.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127964"},"PeriodicalIF":6.1,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-Penetrating Peptides in infection and immunization 细胞穿透肽在感染和免疫中的应用。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-11-05 DOI: 10.1016/j.micres.2024.127963

Yongliang Du , Yan Xiong , Zhou Sha , Dong Guo , Beibei Fu , Xiaoyuan Lin , Haibo Wu

引用次数: 0