Microbiological research最新文献

{"title":"Host phylogeny and traits shape the composition and network structure of the phyllosphere microbial communities in temperate desert plants","authors":"Jun Zhang ,&nbsp;Xiaobing Zhou ,&nbsp;Xiaoying Rong ,&nbsp;Benfeng Yin ,&nbsp;Lei Zhang ,&nbsp;Yuanming Zhang","doi":"10.1016/j.micres.2025.128355","DOIUrl":"10.1016/j.micres.2025.128355","url":null,"abstract":"<div><div>Phyllosphere microorganisms play a vital role in enhancing the adaptability and functionality of their host plants. Although the effects of phyllosphere microbial communities on host functional traits and their association with host phylogeny has been widely investigated, it remains unclear whether host selection consistently drives the assembly of these communities. In this study, bacterial and fungal communities on the surfaces of 734 leaf samples were characterized using bacterial and fungal amplicon sequencing. These microbial communities were associated with 42 plant species native to the Gurbantunggut Desert, a representative temperate desert located in Central Asia. The research assessed the relative contributions of plant-related factors, abiotic environmental variables (such as climate and soil), and spatial components to the observed variation in phyllosphere microbial communities, and further inferred the topological structure of plant-microbe interaction networks. The results indicate that plant phylogeny, plant functional traits, abiotic environment conditions, and spatial factors account for variations in the bacterial community composition (36.4 %, 4.6 %, 1.0 %, and 0.1 %, respectively) and the fungal community composition (28.6 %, 3.0 %, 1.5 %, and 1.2 %, respectively), following a hierarchical trend of plant phylogeny &gt; plant functional traits &gt; abiotic environment &gt; space. Plant phylogeny and functional traits play a central role in shaping the assembly of phyllosphere microbial communities, indicating that plant filtering effects significantly influence microbial composition. Analysis of plant-microbe interactions reveals distinct preferences of microbial taxa for plant hosts across different taxonomic levels and geographic regions. Bipartite network analysis further illustrates that plant-microbe networks are highly specialized and modular, with plant-fungal networks exhibiting greater host specificity compared to plant-bacterial networks. Collectively, these findings underscore plant filtering as the primary determinant of microbial community assembly in the desert phyllosphere and provide valuable insights into the macroecological patterns shaping plant-microbe interactions in arid ecosystems.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128355"},"PeriodicalIF":6.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crosstalk between the m6A modification and the gut microbiota in lipid metabolism","authors":"Haiyan Chen,&nbsp;Yaolin Ren,&nbsp;Jie Yu,&nbsp;Jing Ren,&nbsp;Yuan Zeng,&nbsp;Yifan Wu,&nbsp;Qian Zhang,&nbsp;Xinhua Xiao","doi":"10.1016/j.micres.2025.128356","DOIUrl":"10.1016/j.micres.2025.128356","url":null,"abstract":"<div><div>Lipid metabolism is essential for maintaining cellular homeostasis and human health, and its dysregulation can contribute to metabolic disorders such as obesity and diabetes. As one of the most prevalent RNA modifications, the N6-methyladenosine (m6A) modification plays a pivotal role in regulating gene expression and metabolic pathways. The gut microbiota influences lipid metabolism by modulating the host's m6A modification patterns. Research has shown that the gut microbiota can regulate the levels of the m6A modification in host tissues, while the m6A modification also impacts the composition and functionality of the gut microbiota. This review comprehensively examines the interaction between the m6A modification and the gut microbiota, elucidating its underlying mechanisms in lipid metabolism and highlighting the potential applications of this crosstalk in addressing metabolic diseases. Future investigations should aim to further elucidate the precise molecular mechanisms governing the interplay between the m6A modification and the gut microbiota, thereby providing novel therapeutic targets and strategies for metabolic disease management.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128356"},"PeriodicalIF":6.9,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of ShgH as a dual histidine/glutamine transporter component essential for Streptococcus suis virulence and biofilm modulation","authors":"Carla García ,&nbsp;Luis Saralegui ,&nbsp;Beatriz Morales ,&nbsp;Paula Jurado ,&nbsp;M. Teresa Bes ,&nbsp;Clara Marín ,&nbsp;Jesús Arenas","doi":"10.1016/j.micres.2025.128354","DOIUrl":"10.1016/j.micres.2025.128354","url":null,"abstract":"<div><div><em>Streptococcus suis</em> is a zoonotic pathogen that affects pigs and humans. In this study, we characterised ShgH, a predicted substrate-binding component of an ABC transporter. Immunoassays confirmed that ShgH is expressed, secreted and surface-exposed in <em>S. suis,</em> in agreement with its proposed transporter function. Isothermal titration calorimetry demonstrated that ShgH binds glutamine and histidine, with a higher affinity for histidine. Deletion of the <em>shgH</em> gene significantly impaired uptake of both radiolabelled amino acids confirming its role as part of a transporter. Functional analysis revealed that <em>shgH</em> deletion results in a marked reduction in virulence in a murine infection model, while host colonization remained unaffected. ShgH contributes to infection by facilitating evasion of phagocytosis and resistance to oxidative stress through impaired nutrient acquisition and reduced capsule production. In addition, ShgH regulates biofilm formation and architecture. Notably, ShgH is highly conserved among pathogenic streptococci, suggesting a broader functional relevance. Altogether, our findings identify ShgH as a dual glutamine/histidine- binding protein essential for nutrient uptake and virulence in <em>S. suis</em>, and a promising target for future therapeutic interventions.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128354"},"PeriodicalIF":6.9,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The oxidative stress-related peroxiredoxin Tsa1b of Candidozyma (Candida) auris contributes to virulence and infection","authors":"Maialen Areitio ,&nbsp;Oier Rodriguez-Erenaga ,&nbsp;Leire Aparicio-Fernandez ,&nbsp;Lucía Abio-Dorronsoro ,&nbsp;Leire Martin-Souto ,&nbsp;Uxue Perez-Cuesta ,&nbsp;Idoia Buldain ,&nbsp;Beñat Zaldibar ,&nbsp;Alba Ruiz-Gaitan ,&nbsp;Javier Pemán ,&nbsp;Salomé LeibundGut-Landmann ,&nbsp;Aitor Rementeria ,&nbsp;Aitziber Antoran ,&nbsp;Andoni Ramirez-Garcia","doi":"10.1016/j.micres.2025.128353","DOIUrl":"10.1016/j.micres.2025.128353","url":null,"abstract":"<div><div>The difficulty of accurately identifying <em>Candidozyma auris</em> (formerly <em>Candida auris</em>) and the high resistance rates presented have increased the concern in the healthcare setting. Due to this, the aim of this study was to analyse the fungal response to oxidative stress. To achieve this goal, gene and protein expression were examined using qPCR and two-dimensional electrophoresis, respectively, peroxiredoxin Tsa1b being found to be upregulated under oxidative stress. Subsequently, the significance of Tsa1b was next investigated by characterizing the <em>C. auris</em> Δ<em>tsa1b</em> and <em>C. auris</em> Δ<em>tsa1b::TSA1B</em> strains generated by CRISPR-Cas9. The findings demonstrated that the Δ<em>tsa1b</em> strain was more susceptible to oxidative and cell wall stressors than the wild-type strain, which was consistent with an increase in the cell wall β-glucan amounts when grown in the presence of oxidative stress. Importantly, Tsa1b has also been detected as an antigen by patient sera. Furthermore, the Δ<em>tsa1b</em> strain was also more vulnerable to the presence of dendritic cells and bone marrow-derived macrophages. Finally, <em>in vivo</em> infections performed in <em>Galleria mellonella</em> and mice showed a slower progression of the disease in animals infected with the mutant strain. In conclusion, the peroxiredoxin Tsa1b has been identified as a potential candidate to be studied as a virulence factor implicated in the oxidative stress response of <em>C. auris</em>. These findings advance the knowledge of the pathobiology of <em>C. auris</em> and point out the potential of Tsa1b for the development of new diagnostic and therapeutic approaches.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128353"},"PeriodicalIF":6.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional characterization of chemoreceptors for phenolic acids in Agrobacterium tumefaciens","authors":"Nan Xu,&nbsp;Xiaojing Yang,&nbsp;Chenghao Li,&nbsp;Chen Zhang,&nbsp;Minliang Guo","doi":"10.1016/j.micres.2025.128348","DOIUrl":"10.1016/j.micres.2025.128348","url":null,"abstract":"<div><div>Phenolic acids influence host-pathogen interactions and function as key signals in <em>Agrobacterium</em>-mediated transformation or plant-microbe symbiosis. <em>Agrobacterium tumefaciens</em> uses chemotaxis to detect plant-secreted phenolic compounds and migrates to infection sites, though the chemotactic mechanism remains unclear. In this study, starting with structurally simple phenolic acids, the chemotactic response of <em>A. tumefaciens</em> C58 was investigated. The chemotaxis of <em>A. tumefaciens</em> toward 4-hydroxybenzoate and protocatechuate is not impacted by the methyl-accepting chemotaxis proteins (MCPs) Atu0387 and Atu0738, which share a four-helix bundle domain with previously discovered phenolic-sensing MCPs. To identify chemoreceptors for phenolic acids, a heterologous expression and functional screening system was constructed in <em>Escherichia coli</em>. Among the 13 MCPs, Atu0872 could respond to both 4-hydroxybenzoate and protocatechuate. Furthermore, <em>atu0872</em> deletion weakened chemotaxis toward vanillin, acetosyringone, guaiacol, caffeic, vanillic, salicylic, gallic, <em>p</em>-coumaric, syringic, and sinapinic acids. Although the ligand-binding domain of Atu0872 was predicted to be a nitrate- and nitrite-sensing domain, the <em>A. tumefaciens</em> deletion mutant <em>Δatu0872</em> did not affect chemotaxis toward nitrate and nitrite. In addition to chemotaxis, <em>atu0872</em> deletion decreased the tumor weight on <em>Daucus carota</em> roots, <em>Kalanchoe daigremontiana</em> leaves, and the number of bacterial colonies per 0.1 g of tumor, implying that <em>atu0872</em> affects bacterial colonization on the host by regulating chemotactic behavior. To our knowledge, this is for the first study identifying Atu0872 as a core chemoreceptor in <em>A. tumefaciens</em> for phenolic compounds, providing a theoretical foundation for elucidating the chemotaxis–pathogenicity relationship in <em>A. tumefaciens</em> and optimizing its use in genetic transformations.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128348"},"PeriodicalIF":6.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the interaction between gut microbiota and post-translational modifications of proteins","authors":"Lingling Zhao ,&nbsp;Hengqi He ,&nbsp;Zhaohui Luo ,&nbsp;Liwen Jin ,&nbsp;Bo Xiao","doi":"10.1016/j.micres.2025.128349","DOIUrl":"10.1016/j.micres.2025.128349","url":null,"abstract":"<div><div>Gut microbiota intricately regulate host cells through diverse mechanisms, with numerous pathways involving protein post-translational modifications (PTMs). This review comprehensively summarizes the impacts of the gut microbiota on various PTMs in host cells. It elaborates on how these modifications contribute to the development of host diseases, delving into mediating pathways, including changes in microbial metabolites, key enzymes, and the microenvironment. Conversely, it also explores how PTMs influence gut microbiota abundance. To overcome current research limitations, such as narrow perspectives and monotonous methods, novel strategies are proposed. Applying single-cell/spatial omics could reveal cell-type-specific and spatial PTM responses to microbial signals, while integrating AI algorithms with traditional experiments may predict PTM regulatory networks using microbial and host data. These strategies aim to expand research approaches and promote the clinical translation of findings in this field.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128349"},"PeriodicalIF":6.9,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DL-propargylglycine reverses beta-lactam resistance in Streptococcus agalactiae","authors":"Xuan-wei Chen ,&nbsp;Xiao-lin Zhang ,&nbsp;Zhi-han Wang ,&nbsp;Jia-yao Wu ,&nbsp;Si-qi Tian ,&nbsp;Zi-long Huang ,&nbsp;Bo Peng","doi":"10.1016/j.micres.2025.128350","DOIUrl":"10.1016/j.micres.2025.128350","url":null,"abstract":"<div><div><em>Streptococcus agalactiae</em> (GBS) is a major pathogen causing severe infections in human and economic loss in animal farming, where β-lactams remain first-line therapy. However, emerging β-lactam resistance, including WHO-priority penicillin-resistant strains, threatens clinical efficacy, creating an urgent need for resistance-breaking adjuvants. In this study, we demonstrate that DL-Propargylglycine (PAG), an inhibitor of cystathionine-γ-lyase inhibitor, exclusively synergizes with β-lactams to reverse resistance in ampicillin-resistant GBS (AR-GBS) and other streptococci, overcoming tolerance in persisters and biofilms. Mechanistically, PAG potentiates antibiotic lethality through dual pathways: metabolic activation via enhanced central carbon metabolism for ROS production and cell envelope remodeling via concurrent downregulation of peptidoglycan biosynthesis genes and upregulation of capsular polysaccharide synthesis. This disrupts cell wall architecture, increases membrane permeability and accelerates antibiotic influx. While <em>in vivo</em> therapeutic efficacy in zebrafish was limited, PAG represents an adjuvant that overcomes β-lactam resistance through metabolic and membrane remodeling, paving the way for optimized derivatives.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128350"},"PeriodicalIF":6.9,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterial-driven innovations in phage therapy: Current strategies and future perspectives","authors":"Zihe Zhou ,&nbsp;Mengzhe Li ,&nbsp;Hanyu Fu ,&nbsp;Zhongyu Han ,&nbsp;Zhenchao Wu ,&nbsp;Huahao Fan ,&nbsp;Ning Shen ,&nbsp;Jiajia Zheng","doi":"10.1016/j.micres.2025.128351","DOIUrl":"10.1016/j.micres.2025.128351","url":null,"abstract":"<div><div>The escalating threat of antimicrobial resistance underscores the urgent need for innovative therapeutic strategies. Phage therapy has experienced a resurgence over the past five years following a prolonged period of neglect during the antibiotic era. Despite its therapeutic promise, critical barriers impede clinical translation, including susceptibility to interference from the host's complex physiological environment, a narrow host range, and the inability to lyse intracellular bacteria. To address these limitations and optimize the efficacy of phage-mediated treatment, recent research has increasingly focused on biomaterial-assisted approaches aimed at enhancing therapeutic efficacy. In this review, we concentrate on recent progress in biomaterial-assisted phage-based treatment strategies, including phage physical encapsulation strategies and phage surface chemical coupling strategies. Physical encapsulation employs liposomes, hydrogels, pH-sensitive polymers and etc. for controlled phage delivery, while surface chemical coupling modifies phage capsids with photosensitizers, nanozymes, or metal nanoparticles to enable multifunctional bactericidal mechanisms. In addition, accessibility for phage therapy of intracellular bacteria is discussed. We also conclude key biomaterial selection criteria-prioritizing biosafety, biodegradability, and microenvironment adaptability, and offer novel perspectives for advancing therapeutic precision as well as multidimensional innovation in combating antimicrobial resistance.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128351"},"PeriodicalIF":6.9,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridium butyricum attenuates radiation-induced bone loss through gut microbiota and immune regulation in mice","authors":"Jinmin Lv ,&nbsp;Hao Chen ,&nbsp;Yichao Ni ,&nbsp;Yue Zhang ,&nbsp;Xingrui Huang","doi":"10.1016/j.micres.2025.128352","DOIUrl":"10.1016/j.micres.2025.128352","url":null,"abstract":"<div><div>Radiation-induced bone loss remains clinically challenging due to limitations of existing treatments. Using a mouse model of fractionated total abdominal irradiation (TAI), we demonstrate that TAI triggers severe osteoporosis characterized by trabecular bone loss, suppressed osteogenesis, and elevated osteoclast activity. Through 16S rRNA sequencing and immune profiling, we found TAI induced gut dysbiosis, immune dysregulation such as Th17/Treg imbalance, and systemic inflammation. Oral supplementation with <em>C. butyricum</em> reversed bone loss, activated osteogenic signaling, suppressed osteoclastogenesis, and rebalanced T-cell subsets in mice. Crucially, it restored gut microbiota composition and attenuated inflammation in intestinal and bone marrow microenvironments. Our findings establish <em>C. butyricum</em> as a gut microbiota-targeted therapy for radiation-induced bone loss via the gut-immune-bone axis.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128352"},"PeriodicalIF":6.9,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citrus fruit microbiome changes under copper-based and biological alternative treatments and its biocontrol potential","authors":"M.F. Lombardo ,&nbsp;A. Abdelfattah ,&nbsp;V. Catara ,&nbsp;N. Wang ,&nbsp;G. Cirvilleri","doi":"10.1016/j.micres.2025.128346","DOIUrl":"10.1016/j.micres.2025.128346","url":null,"abstract":"<div><div>Citrus, a globally significant fruit crop, harbours a distinctive microbial community crucial for maintaining citrus health and enhancing disease resistance. While the structure and shaping factors, including phytosanitary treatments, of citrus root and leaf microbiomes are well documented, the carposphere (fruit surface) microbiome and its response to phytosanitary inputs remain poorly understood. In the present study, we combined culture independent (amplicon sequencing) and culture dependent techniques to analyse the citrus carposphere microbiome across three citrus hosts and its response to field-applied phytosanitary treatments (biologicals and copper-antimicrobials). Despite host-specific variation in the relative abundance of dominant taxa such as Proteobacteria, Firmicutes, and Basidiomycota, all three citrus hosts shared a core microbiome, consistently present across fruit samples. Bacterial diversity and composition were negatively influenced by copper treatments, whereas biological products (chitosan, sweet orange essential oils and their mixtures) had minimal or no negative impacts. Fungal communities, including potential pathogens, appeared less sensitive to treatments. Network analysis confirmed that copper altered microbial interactions, increasing mutual exclusion relationships between bacterial taxa compared to untreated or biologically treated samples, which were dominated by positive interactions. A parallel survey of cultivable microbiota from the same samples identified potential biocontrol agents (BCAs) against <em>Colletotrichum gloeosporioides</em> and <em>Alternaria alternata</em>. Notably, cross-referencing cultivable BCAs with core Amplicon Sequence Variants (ASVs) showed that 81.7 % of bacterial core members represent potential biocontrol agents. This study highlights the importance of management practices for sustaining beneficial microbiomes. Furthermore, it establishes a valuable resource of core-associated BCAs, offering promising avenues for the biological control of fungal pathogens.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"302 ","pages":"Article 128346"},"PeriodicalIF":6.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}