Microbiological research最新文献

{"title":"Identification and characterization of a novel bacteriocin PCM7-4 and its antimicrobial activity against Listeria monocytogenes","authors":"Haotian Ma ,&nbsp;Yuexia Ding ,&nbsp;Jinju Peng ,&nbsp;Yang Li ,&nbsp;Ruixue Pan ,&nbsp;Yuner Long ,&nbsp;Yining Zhao ,&nbsp;Rongxian Guo ,&nbsp;Yi Ma","doi":"10.1016/j.micres.2024.127980","DOIUrl":"10.1016/j.micres.2024.127980","url":null,"abstract":"<div><div><em>Listeria monocytogenes</em>, a pathogenic bacterium causing zoonotic diseases, necessitates the urgent search for novel anti-<em>Listeria monocytogenes</em> drugs due to the continuous emergence of drug-resistant bacteria. In this study, we isolated and identified a bacteriocin-producing strain CM7–4 from seawater as <em>Bacillus velezensis</em> through 16S rRNA sequence analysis. Moreover, we successfully purified a novel bacteriocin named PCM7–4 from <em>Bacillus velezensis</em> CM7–4. The molecular weight of PCM7–4 was determined to be 40,228.99 Da. Notably, PCM7–4 exhibited broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria with a minimum inhibitory concentration (MIC) of 5.625 μg/mL against <em>Listeria monocytogenes</em> specifically. It demonstrated heat resistance and high stability within the pH range of 2–12 while being sensitive to proteinase K degradation without any observed hemolytic activity. Furthermore, SEM analysis revealed that PCM7–4 effectively inhibited biofilm formation and disrupted cell membranes in <em>Listeria monocytogenes</em> cells. Transcriptome analysis revealed that PCM7–4 exerts an impact on genes associated with crucial metabolic pathways, encompassing the biosynthesis of secondary metabolites, phosphotransferase systems (PTS), and starch/sucrose metabolism. These findings highlight the significant potential of bacteriocin PCM7–4 for the development of effective antimicrobial interventions targeting food-borne pathogenic bacteria.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127980"},"PeriodicalIF":6.1,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Furamidine-induced autophagy exerts an anti-mycobacterial effect in a SIRT1-pAMPK-FOXO3a-dependent manner by elevation of intracellular Ca2+ level expression","authors":"Salina Patel ,&nbsp;Lincoln Naik ,&nbsp;Mousumi Das ,&nbsp;Dev Kiran Nayak ,&nbsp;Pramathesh Kumar Dandsena ,&nbsp;Abtar Mishra ,&nbsp;Ashish Kumar ,&nbsp;Vijaya R. Dirisala ,&nbsp;Amit Mishra ,&nbsp;Surajit Das ,&nbsp;Ramandeep Singh ,&nbsp;Assirbad Behura ,&nbsp;Rohan Dhiman","doi":"10.1016/j.micres.2024.127976","DOIUrl":"10.1016/j.micres.2024.127976","url":null,"abstract":"<div><div><em>Mycobacterium tuberculosis</em> (<em>M. tb</em>), the etiological agent of tuberculosis (TB), continues to be a major contributor to global mortality rates. To effectively combat this pandemic, TB control has to be enhanced in several areas, including point-of-care diagnostics, shorter and safer drug regimens, and preventative vaccination. The latest findings have highlighted autophagy as a host-defense mechanism that eradicates many invading bacteria, including <em>M. tb.</em> Thus, novel approaches like the stimulation of autophagy using various pharmaceutical drugs can be undertaken to deal with this noxious pathogen. The present study has been formulated to evaluate the anti-mycobacterial potential of Furamidine, a DNA minor groove binder (MGB). Initially, a non-cytotoxic concentration of Furamidine (10 µM) was used to assess its impact on the intracellular persistence of mycobacteria in differentiated THP-1 (dTHP-1) cells. Furamidine treatment compromised intracellular mycobacterial growth compared to control cells. Autophagy, a well-known host-defensive strategy, was investigated as a possible contributor to revealing the mechanism of action. Multiparametric approaches such as LC3-I to II conversion, protein level expression of different autophagic markers, and MDC staining were employed to study autophagic response that conclusively suggested the autophagy induction potential of Furamidine in dTHP-1 cells. Further, elevated LC3-II expression and increased autophagic vacuole accumulation under Baf-A1 treatment demonstrated the positive regulation of autophagic flux upon Furamidine treatment. Mechanistic investigations showed increased intracellular calcium (Ca²⁺) level expression, SIRT1, pAMPK, and FOXO3a activation upon its treatment. Inhibition of Ca²⁺ level expression suppressed Ca²⁺-mediated-FOXO3a level in Furamidine-treated cells. Furthermore, administering various inhibitors hampered the Furamidine-induced autophagy that impacted intracellular mycobacteria clearance. These results conclude that Furamidine triggered the Ca²⁺/pAMPK/SIRT1/FOXO3a pathway, causing less mycobacterial load in dTHP-1 cells.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127976"},"PeriodicalIF":6.1,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Division mechanism of labor in Diqing Tibetan Pigs gut microbiota for dietary fiber efficiently utilization","authors":"Lijie Yang ,&nbsp;Bingqian Yao ,&nbsp;Shimin Zhang ,&nbsp;Yuting Yang ,&nbsp;Gang Wang ,&nbsp;Hongbin Pan ,&nbsp;Xiangfang Zeng ,&nbsp;Shiyan Qiao","doi":"10.1016/j.micres.2024.127977","DOIUrl":"10.1016/j.micres.2024.127977","url":null,"abstract":"<div><div>The Diqing Tibetan (TP) pig is an roughage tolerance breed that inhabits an area with the highest altitude distribution in the world and can be maintained on a diet containing 90 % forage material in confined production systems. Our results showed that TP pigs had a strong capability for high-efficiency utilization of arabinose and xylose. Metagenomic analysis revealed that the secretion of carbohydrate esterases was mainly undertaken by fecal strains of <em>Microbacterium</em>, <em>Alistipes</em>, <em>Acinetobacter</em>, and <em>Faecalibacterium</em>, while <em>Microbacterium</em>, <em>Prevotella</em>, <em>Turicibacter</em>, <em>Lactobacillus</em>, <em>Clostridium</em>, and <em>Streptococcus</em> were responsible for most of the secretion of glycoside hydrolases. Then, a brand new species, which was named <em>Microbacterium sp. Qiao 01</em> was captured and appeared to have the highest fiber utilization ability <em>in vitro</em>, degrading 36.54 % of the neutral detergent fiber in corn stover. Our results provide strong evidence that efficient utilization of dietary fiber by TP pigs is due to the emergence of highly specialized microbial strategies in the gut. Microorganisms showed preferences and a clear division of labor in the degradation process of dietary fiber. This study has great practical significance for improving the utilization efficiency of livestock feed and alleviating the tension of food insecurity.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127977"},"PeriodicalIF":6.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agricultural relevance of fungal mycelial growth-promoting bacteria: Mutual interaction and application","authors":"Toga Pangihotan Napitupulu","doi":"10.1016/j.micres.2024.127978","DOIUrl":"10.1016/j.micres.2024.127978","url":null,"abstract":"<div><div>Bacterial-fungal interaction (BFI) is found ubiquitously and plays important roles in various environmental settings, thus being responsible for numerous biophysical and chemical processes in nature. In terms of BFI, the capacity of the bacterium to enhance the growth of fungal mycelia is an indication of the roles of the bacterium in mutualistic interaction, since increasing mycelial growth results in higher changes for fungal establishment. In this review, the interaction between mycelial growth-promoting bacterium (MGPB) and its fungal counterpart in agricultural settings and the promotion of mycelial growth as an outcome of mutual interactions in various environmental niches were evaluated. The beneficial relationships included endohyphal interaction, association of bacteria with mushrooms, bacteria-mycorrhizae symbiosis, and geomicrobiology. Furthermore, the mode of interaction between MGPB and their fungal counterparts was also explained. There are two fundamental modes of interaction involved, namely physical interaction and chemical interaction. The first involved endosymbiosis and bacterial attachment, while the latter comprised quorum sensing, volatile metabolites, enzymatic activity, and chemotaxis. Particularly, the growth stimulants secreted by the bacteria, which promote the growth of hyphae, are discussed thoroughly. Moreover, the chance of trade-off metabolites between fungi and their MGPBs as a consequence of mutualistic interaction will also be observed. Finally, the agricultural relevance of BFI, particularly the relation between fungi and MGPBs, will also be provided, including key technologies and future bioprospects for optimum application.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127978"},"PeriodicalIF":6.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synechococsins: Lanthipeptides acting as defensive signals to disarm offensive competitors?","authors":"Patricia Arias-Orozco ,&nbsp;Rubén Cebrián ,&nbsp;Anne de Jong ,&nbsp;Oscar P. Kuipers","doi":"10.1016/j.micres.2024.127965","DOIUrl":"10.1016/j.micres.2024.127965","url":null,"abstract":"<div><div>Synechococsins represent a diverse group of class II lanthipeptides from the prochlorosin family, produced by the marine picocyanobacterium <em>Synechococcus.</em> A single strain can produce multiple SyncA peptides through modification by SyncM, a bifunctional lanthipeptide synthetase. Despite the prevalence of these lanthipeptides in nature, their biological functions remain elusive, even for the most studied group, <em>Prochlorococcus</em> MIT9313. This study investigated the transcriptomic response of the marine SyncA-producing strain <em>Synechococcus</em> sp. RS9116 to the characterized and purified SyncA6 peptide from <em>Synechococcus</em> sp. MITS9509. Intriguingly, the analysis of gene expression revealed a strong down-regulation of genes that encode putative ribosomally produced antimicrobial peptides, such as coculture-responsive genes (CCRG-2) and microcin-C-like bacteriocins. This study suggests a potential biological role for synechococsins as interspecific gene modulators, improving the fitness of the producing strain in a competitive and resource-limited environment.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"291 ","pages":"Article 127965"},"PeriodicalIF":6.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adapted evolution towards flagellar loss in Pseudomonas syringae","authors":"Jiarong Wang ,&nbsp;Xiaoquan Yu ,&nbsp;Hao Yang ,&nbsp;Hanzhong Feng ,&nbsp;Yujuan Wang ,&nbsp;Nannan Zhang ,&nbsp;Haining Xia ,&nbsp;Jie Li ,&nbsp;Lei Xing ,&nbsp;Junfeng Wang ,&nbsp;Yongxing He","doi":"10.1016/j.micres.2024.127969","DOIUrl":"10.1016/j.micres.2024.127969","url":null,"abstract":"<div><div>The flagellum is a complex molecular nanomachine crucial for cell motility. Its assembly requires coordinated expression of over 50 flagellar genes, regulated by the transcription activator FleQ. Phylogenomic analyses suggest that many non-flagellated bacterial species have evolved from flagellated ancestors by losing specific flagellar components, though the evolutionary mechanisms driving this process remain unclear. In this study, we examined the evolutionary dynamics of <em>Pseudomonas syringae</em> DC3000 under standard laboratory conditions using quantitative proteomics. We observed a notable reduction in flagellar gene expression following prolonged serial passages. Whole-genome sequencing revealed multiple adaptive mutations in <em>fleQ</em>, <em>dksA</em>, and <em>glnE</em>, all of which are associated with flagellar biosynthesis. Furthermore, our findings demonstrate that nonmotile Δ<em>fleQ</em> cells can hitchhike onto wild-type cells, potentially facilitated by increased production of the surfactant syringafactin. Our study suggests that the high metabolic costs associated with flagella biosynthesis, coupled with advantageous hitchhiking properties, contribute to the degenerative evolution of flagella.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127969"},"PeriodicalIF":6.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibody-based therapy: An alternative for antimicrobial treatment in the post-antibiotic era","authors":"Liang Luo ,&nbsp;Qing Li ,&nbsp;Chen Xing ,&nbsp;Chenglong Li ,&nbsp;Yantong Pan ,&nbsp;He Sun ,&nbsp;Xuezhi Yu ,&nbsp;Kai Wen ,&nbsp;Jianzhong Shen ,&nbsp;Zhanhui Wang","doi":"10.1016/j.micres.2024.127974","DOIUrl":"10.1016/j.micres.2024.127974","url":null,"abstract":"<div><div>The consecutive growth of antimicrobial resistance and the spread of resistance genes worldwide, especially the emergence of superbugs, have made traditional antibiotic-based treatments inadequate to fight bacterial infections. Therefore, new therapeutic modalities for bacterial infections are urgently needed. Antibodies are considered to be an effective alternative to antibiotics. The emergence and advancement of technologies such as hybridoma, antibody purification, transgenic mice, phage display, and protein engineering have enabled the production of large quantities of humanized antibodies with high purity and affinity. Antibodies has achieved remarkable achievements in the field of medicine in the past decades. Antibody-based therapy is expected to be an effective way to treat drug-resistant bacterial infections in the post-antibiotic era due to its merits of high specificity, which leads to no selective pressure on non-target bacteria and could cooperate with antibiotics to enhance the antimicrobial effect. This review first introduces the mechanism of action of antibodies against bacterial infections, then summarizes the reported antimicrobial antibodies according to different targets, discusses the advantages and limitations of the antibody-based therapy for antimicrobial treatment, and finally, the perspectives of antimicrobial antibodies developing have been prospected, providing a reference for the development of new antimicrobial antibodies.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127974"},"PeriodicalIF":6.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus paragasseri SBT2055 attenuates obesity via the adipose tissue-muscle-gut axis in obese mice","authors":"Min-Jeong Kim ,&nbsp;Su-Kyung Shin ,&nbsp;Ji-Won Han ,&nbsp;Ji Eun Kim ,&nbsp;Min Jee Lee ,&nbsp;Heekyong R. Bae ,&nbsp;Eun-Young Kwon","doi":"10.1016/j.micres.2024.127972","DOIUrl":"10.1016/j.micres.2024.127972","url":null,"abstract":"<div><div>The anti-obesity effects of <em>Lactobacillus paragasseri</em> (<em>L. paragasseri</em>) have been reported, but the exact mechanisms have not been elucidated. There are also no reports on the impact of <em>L. paragasseri</em> on the gut microbiota environment. Recently, the incidence of sarcopenia due to obesity has increased regardless of age, exacerbating metabolic disorders caused by obesity. Therefore, we investigate the beneficial effects of <em>L. paragasseri</em> SBT2055 (<em>LG2055</em>) on obesity along with obese sarcopenia and gut microbiome changes. C57BL/6 J mice were fed a high-fat diet (HFD) and <em>LG2055</em> (1×10⁸ or 1×10¹⁰ CFU/mice, low-dose LG2055 (LP) or high-dose LG2055 (HP), respectively was administered orally. <em>LG2055</em> supplementation significantly reduced white adipose tissues compared to the HFD group and modified plasma lipid profiles to normal levels. The anti-obesity efficacy of <em>LG2055</em> was due to increased lipid excretion into feces by reducing the mRNA levels of fatty acid binding protein 1 (Fabp1), fatty acid binding protein 2 (Fabp2), fatty acid transport protein 4 (Fatp4), cluster of differentiation 36 (Cd36), and apolipoprotein 48 (ApoB48) in the small intestine. The body fat reduction inhibits ectopic lipid accumulation in the muscles, leading to improvements in muscle mass, grip strength, hind leg thickness, muscle protein levels, and muscle fiber size in both LP and HP groups. <em>LG2055</em> increased gut microbiota diversity and elevated the levels of <em>Bacteroidota</em>, resulting in a lower <em>Firmicutes/Bacteroidota</em> ratio compared to the HFD group. Changes in the <em>Bacteroidota</em> showed a negative correlation with body fat and plasma free fatty acid (FFA) while exhibiting a positive correlation with lean body mass, grip strength, and hind leg thickness. Our results demonstrated the anti-obesity effects of <em>LG2055</em> through the white adipose tissue (WAT)-muscle-gut axis, suggesting its potential as an anti-obesity agent.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127972"},"PeriodicalIF":6.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Xanthomonas growth by bioactive volatiles from Pseudomonas sp. triggers remarkable changes in the phytopathogen transcriptome","authors":"Luciane Fender Coerini ,&nbsp;Aline Tieppo Nogueira Mulato ,&nbsp;Joaquim Martins-Junior ,&nbsp;Gabriela Felix Persinoti ,&nbsp;Juliana Velasco de Castro Oliveira","doi":"10.1016/j.micres.2024.127971","DOIUrl":"10.1016/j.micres.2024.127971","url":null,"abstract":"<div><div>Volatile organic compounds (VOCs) produced by microorganisms may have a noteworthy role in the control of plant pathogens. <em>Xanthomonas</em> are a well-studied group of phytobacteria that cause diverse diseases in economically important crops worldwide. Key species that infect sugarcane are <em>X. albilineans</em> (Xab) and <em>X. axonopodis</em> pv. <em>vasculorum</em> (Xav)<em>.</em> Here, we investigated VOC-producing bacteria with antagonistic effects against Xab and Xav. We demonstrated that VOCs produced by <em>Pseudomonas</em> sp. V5-S-D11 was able to abolish the growth of these pathogens. A set of 32 VOCs was identified in the volatilome of V5-S-D11, with 10 showing a concentration-dependent inhibitory effect on both phytobacteria<em>.</em> Among them, dimethyl disulfide (DMDS), a volatile sulfur compound, has the potential to be biotechnologically explored in agriculture since it can improve plant growth and induce systemic resistance against plant pathogens. Interestingly<em>,</em> transcriptomic analysis of Xab treated with DMDS revealed several up-regulated metabolic pathways such as a two-component system, flagellar assembly, chemotaxis, and a bacterial secretion system. Although the ethanol (ETOH) used as DMDS solvent did not inhibit Xab growth, it triggered a similar up-regulation of some genes, indicating that this phytopathogen can deal with ETOH better than DMDS. Overall, this study explores the wide role of VOCs in the interactions with bacteria. Moreover, our results indicate that VOCs from <em>Pseudomonas</em> sp. may represent a novel biotechnological strategy to counteract diseases caused by <em>Xanthomonas</em> species and can be further exploited for sustainable approaches in agriculture.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127971"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of gut microbiota in rheumatoid arthritis: Potential cellular mechanisms regulated by prebiotic, probiotic, and pharmacological interventions","authors":"Jiashang Li,&nbsp;Ruoying Fan,&nbsp;Zhe Zhang,&nbsp;Lihui Zhao,&nbsp;Yu Han,&nbsp;Yue Zhu,&nbsp;Jin-ao Duan,&nbsp;Shulan Su","doi":"10.1016/j.micres.2024.127973","DOIUrl":"10.1016/j.micres.2024.127973","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a chronic autoimmune disease that primarily affects joints and multiple organs and systems, which is long-lasting and challenging to cure and significantly impacting patients' quality of life. Alterations in the composition of intestinal flora in both preclinical and confirmed RA patients indicate that intestinal bacteria play a vital role in RA immune function. However, the mechanism by which the intestinal flora is regulated to improve the condition of RA is not fully understood. This paper reviews the methods of regulating gut microbiota and its metabolites through prebiotics, probiotics, and pharmacological interventions, and discusses their effects on RA. Additionally, it explores the potential predictive role of cellular therapy mechanisms of intestinal flora in treating RA. These findings suggest that restoring the ecological balance of intestinal flora and regulating intestinal barrier function may enhance immune system function, thereby improving rheumatoid arthritis. This offers new insights into its treatment.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127973"},"PeriodicalIF":6.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}