Microbiological research最新文献

{"title":"C28-aldehyde (n-octacosanal) modulates stage-specific temporal expression of effector genes in the wheat powdery mildew fungus","authors":"Mo Zhu ,&nbsp;Wanwan Zhang ,&nbsp;Fuhai Zhang ,&nbsp;Xiao Duan ,&nbsp;Zongbo Qiu ,&nbsp;Sujing Zhao ,&nbsp;Shiqiang Gao ,&nbsp;Fei He","doi":"10.1016/j.micres.2025.128311","DOIUrl":"10.1016/j.micres.2025.128311","url":null,"abstract":"<div><div>The prepenetration processes of the wheat powdery mildew fungus, <em>Blumeria graminis</em> f. sp. <em>tritici</em> (Bgt), are triggered by C₂₈ aldehyde (<em>n</em>-octacosanal), a component of cuticular waxes. Despite being the most severe crop disease worldwide, the underlying molecular mechanisms of the prepenetration processes remain obscure. Utilizing a Formvar®-based <em>in vitro</em> system, transcriptomes of Bgt conidia impacted by <em>n</em>-octacosanal were profiled without the effects from plant host. A total of 1354 differentially expressed genes were identified between <em>n</em>-octacosanal- and <em>n</em>-octacosane (non-chemical signal)-treated conidia. The expression of effectors, transcription factors, and HOG-MAPK pathways is specifically regulated by <em>n</em>-octacosanal in a developmental stage-dependent manner. Among them, 25 effectors and three transcription factors, including COD 1, VEA, and CreA, were highly expressed at all stages. While some genes of the HOG-MAPK pathway were significantly upregulated during conidial growth, other genes were downregulated. These results revealed that C₂₈ aldehyde-triggered Bgt conidial prepenetration in the plant host might be achieved by activating specific transcription factors and differentially regulating the HOG-MAPK pathway. The genes detected by our gene expression analysis may be crucial for successful infection by Bgt and thus serve as candidates for future functional analysis of the molecular mechanisms of conidia development in powdery mildew. These findings provide new insights into the chemical-signal-orchestrated development of an important phytopathogenic fungus and will potentially support efforts for the control and management of fungal diseases in wheat.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128311"},"PeriodicalIF":6.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pirin, a redox-sensitive modulator of beta-oxidation, generates hydroxyl radicals and interacts with CatR, the transcriptional repressor of the major vegetative catalase gene in Streptomyces","authors":"Matteo Calcagnile ,&nbsp;Fabrizio Damiano ,&nbsp;Adelfia Talà ,&nbsp;Pietro Alifano","doi":"10.1016/j.micres.2025.128310","DOIUrl":"10.1016/j.micres.2025.128310","url":null,"abstract":"<div><div>Pirins are iron-containing proteins conserved throughout evolution, which have been implicated in diverse cellular processes, mostly associated with stress. In prokaryotes, Pirins are present in many taxonomic groups and can be present in multiple copies, and only a few of these proteins have been studied. In <em>Streptomyces ambofaciens</em> a Pirin-like protein, PirA, is a redox-sensitive negative modulator of AcdB, a very long-chain acyl-CoA dehydrogenase (vLCAD), which catalyzes the first committed step of the beta-oxidation pathway. In this study, we first classified the Pirins in different prokaryotic and non-prokaryotic taxa, and we found strong connections between the occurrence of Pirins and aerobic energy metabolism. We then studied whether the presence of Pirins is connected to the regulation of antioxidant systems, after observing that a <em>pirA</em>-defective mutant of <em>S. ambofaciens</em> accumulated large amounts of H₂O₂ during the vegetative growth. <em>In vitro</em> experiments suggested that the accumulation of H₂O₂ in the <em>pirA</em> mutant could be partially due to an increased vLCAD activity of AcdB, which releases H₂O₂ as a byproduct of the reaction. However, the reduction in catalase and alkylhydroperoxidase expression levels in the <em>pirA</em> mutant, despite the increased amount of intracellular H₂O₂, also indicated a dysregulation of these antioxidant systems. Indeed, the gene <em>catA</em> encoding the major vegetative catalase and the adjacent regulatory gene <em>catR</em> were down-regulated in the <em>pirA</em> mutant. In Gram-positive bacteria, CatR/PerR family regulators repress transcription of genes coding for the major vegetative catalase. These repressors are irreversible inactivated by hydroxyl radicals and detach from the DNA, which leads to de-repression of catalase expression. We found that PirA generates hydroxyl radicals after reacting with H₂O₂, and binds CatR <em>in vitro</em>, suggesting that by generating hydroxyl radicals in response to H₂O₂ exposure, PirA could promote CatR inactivation allowing <em>catA</em> transcription.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128310"},"PeriodicalIF":6.9,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the interactions of gut microbiome and metabolites in polycystic ovary syndrome: a review","authors":"Jueyu Li ,&nbsp;Yuqi Jia ,&nbsp;Haojie Xu ,&nbsp;Hao Bing ,&nbsp;Qixian Ling ,&nbsp;Sihan Wang ,&nbsp;Yanan Wang ,&nbsp;Xianhua Zhang ,&nbsp;Libo Zhao","doi":"10.1016/j.micres.2025.128309","DOIUrl":"10.1016/j.micres.2025.128309","url":null,"abstract":"<div><div>Polycystic ovary syndrome (PCOS) is a prevalent endocrine metabolic disorder significantly impacting female health. While its etiology remains incompletely understood, emerging evidence highlights the gut microbiome as a key modulator, affecting not only the gut but also physiology beyond it. Many studies have been committed to exploring the role played by the gut microbiome and have discovered that gut microbiome is commonly dysregulated in PCOS patients. In this review, we offered an introduction to PCOS and summarized the high-quality research in recent years to elucidate the complex interactions between endogenous metabolites with the gut microbiome in PCOS. Furthermore, we also discuss microbiome-targeting therapeutic strategies. By integrating current knowledge on the gut microbiome-metabolite axis, this review aims to provide novel insights and perspectives for future research and potential management of PCOS.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128309"},"PeriodicalIF":6.9,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypovirulence induced by mycovirus colletotrichum gloeosporioides RNA virus 1 strain Ssa-44.1 in Colletotrichum gloeosporioides: Insights from a multi-omics analysis of host-virus interactions","authors":"Muhammad Kabir Hassan ,&nbsp;Liying Sun ,&nbsp;Jiraporn Jirakkakul ,&nbsp;Treenut Saithong ,&nbsp;Saowalak Kalapanulak ,&nbsp;Sucheewin Krobthong ,&nbsp;Arnatchai Maiuthed ,&nbsp;Yingchutrakul Yodying ,&nbsp;Bayu Hadi Permana ,&nbsp;Lakha Salaipeth","doi":"10.1016/j.micres.2025.128308","DOIUrl":"10.1016/j.micres.2025.128308","url":null,"abstract":"<div><div>Mycovirus infections significantly impact fungal virulence and physiology, inducing either hypovirulence or hypervirulence. This study investigated the hypovirulent effects of Colletotrichum gloeosporioides RNA virus 1 (CgRV1-Ssa-44.1) infection on <em>Colletotrichum gloeosporioides</em> using multi-omics approaches. Transcriptomic analysis identified 261 differentially expressed genes (141 up-regulated, 120 down-regulated), while LC-MS/MS-based proteomic analyses revealed 2222 proteins, including 19 unique to virus-infected samples and 649 unique to virus-free samples. These results highlighted extensive gene and protein expression alterations, emphasizing profound impacts on the host cellular process. Changes in membrane-associated terms and cell wall-related processes suggested that the virus may exploit host structures to facilitate horizontal transfer. The disruption of carbohydrate metabolism and pathways, such as the non-sense mediated mRNA decay (NMD) system, reflected sophisticated viral strategies for suppressing host defenses and redirecting resources for its benefit. Notably, Upregulated genes, such as sorbose reductase and COMPASS complex component SWD2, pointed to adaptive response to stress and survival mechanisms during viral infection. Conversely, downregulated genes like elongation factor 3, survival factor 1, and zuotin, indicated viral manipulation of host cellular machinery to subvert normal processes. Real-time PCR validated these transcriptional changes, confirming the robustness of the findings. The study demonstrates a complex host-virus interplay, where fungal metabolic and adaptive pathways are intricately targeted and exploited. These findings underscore the dual nature of viral subversion strategies, balancing host suppression with survival adaptation. Future functional analyses of key pathways will provide insights into the molecular mechanisms underlying fungal-virus interactions and coevolution. This knowledge could guide the development of novel antifungal strategies applicable to similar host-pathogen systems.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128308"},"PeriodicalIF":6.9,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144826643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Native Australian plants to combat oral health-related pathogens","authors":"Ratih Pusporini ,&nbsp;Maral Seididamyeh ,&nbsp;Anh Dao Thi Phan ,&nbsp;Run Zhang ,&nbsp;Chun Xu ,&nbsp;Yasmina Sultanbawa","doi":"10.1016/j.micres.2025.128307","DOIUrl":"10.1016/j.micres.2025.128307","url":null,"abstract":"<div><div>Dental caries remains one of the most prevalent chronic diseases worldwide, impacting health, quality of life, and child development, while posing a substantial economic burden. The rise of antimicrobial resistance has intensified the search for alternative preventive strategies, particularly those derived from natural products. Recent studies highlight that Australian native plants possess significant antimicrobial, antioxidant, and phytochemical properties, suggesting their potential as natural alternatives to synthetic agents.</div><div>This review evaluates the therapeutic potential of Australian native plants in preventing dental caries. It examines their bioactive compounds, mechanisms of antimicrobial action, and their application in oral health and dentistry. The review also discusses the traditional and commercial uses of these plants and outlines future research directions.</div></div><div><h3>Findings</h3><div>reveal that their bioactivity is primarily attributed to phytochemical compounds, which exert antimicrobial, anti-inflammatory, and antioxidant effects—key mechanisms in caries prevention.</div><div>Australian native plants demonstrate promising potential as natural therapeutic agents for dental caries prevention. Their incorporation into oral health strategies may contribute to innovative, sustainable solutions in natural product-based drug discovery.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128307"},"PeriodicalIF":6.9,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144810477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyromonas gingivalis outer membrane vesicles promote oral tumorigenesis through suppressing innate immune surveillance","authors":"Qingli Chen ,&nbsp;Xiaochan Pang ,&nbsp;Ke Liu ,&nbsp;Hui Wang ,&nbsp;Tengyang Qiu ,&nbsp;Guo-Feng Luo ,&nbsp;Zhengjun Shang","doi":"10.1016/j.micres.2025.128296","DOIUrl":"10.1016/j.micres.2025.128296","url":null,"abstract":"<div><div>Several studies have linked microbes to the development of neoplasms, with species of the genus <em>Porphyromonas</em> being closely associated with the progression of oral squamous cell carcinoma (OSCC). However, the immunomodulatory role of <em>Porphyromonas gingivalis</em> (<em>P. gingivalis</em>) outer membrane vesicles (OMVs) in OSCC progression remains unclear. Here, we found that <em>Porphyromonas</em> was significantly enriched in head and neck squamous cell carcinoma, including OSCC tissues. The increased abundance of <em>P. gingivalis</em> in OSCC was confirmed in both saliva and tumor tissues, and correlated with advanced clinical stages. The uptake of <em>P. gingivalis</em> OMVs by OSCC cells was assessed using both <em>in vitro</em> and <em>in vivo</em> models. The OMVs inactivated the cyclic GMP–AMP synthase (cGAS)–stimulator of interferon genes (STING) innate immune signaling, thereby disrupting cytokine production. Herring testis DNA treatment partially restored pIRF3 translocation and induce IFN-β production <em>in vivo</em>. Mechanistically, <em>P. gingivalis</em> OMVs remarkably disrupted both the IRF3–STING and IRF3–TBK1 interaction, consequently blocking the downstream signaling. In both immunodeficient (BALB/c-nu) and immunocompetent (C57BL/6) mouse models, <em>P. gingivalis</em> OMVs suppressed cGAS-STING innate immune signaling, impairing IFN-β production and promoting immune evasion by limiting the recruitment of natural killing cell and dendritic cell to the tumor microenvironment. These findings suggest that <em>P. gingivalis</em> OMVs suppress cGAS–STING–IFN-β innate immune signaling, thereby impairing antitumor immunity.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128296"},"PeriodicalIF":6.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Type IV-A CRISPR/Cas system and plasmid-mediated transmission of carbapenemase genes in Klebsiella pneumoniae","authors":"Jinzhao Long ,&nbsp;Jie Wu ,&nbsp;Yanyan Xi ,&nbsp;Jiangfeng Zhang ,&nbsp;Shuaiyin Chen ,&nbsp;Haiyan Yang ,&nbsp;Guangcai Duan","doi":"10.1016/j.micres.2025.128297","DOIUrl":"10.1016/j.micres.2025.128297","url":null,"abstract":"<div><div>The global rise of carbapenem-producing <em>K. pneumoniae</em> is largely attributed to plasmid-mediated transmission of carbapenemase genes. Type IV-A CRISPR/Cas system is mainly located on plasmids in <em>K. pneumoniae</em> and involved in plasmid competition. However, the role of Type IV-A system in the dissemination of carbapenemase genes in <em>K. pneumoniae</em> remains unclear<em>.</em> Here, we comprehensively investigated the relationship between Type IV-A system and plasmid-mediated transmission of carbapenemase genes based on 152 <em>K. pneumoniae</em> clinical strains and 46226 <em>K. pneumoniae</em> public genomes available in NCBI database. We found that the presence of Type IV-A system was positively associated with <em>bla</em>_NDM-1, <em>bla</em>_NDM-5, <em>bla</em>_OXA-48, and <em>bla</em>_VIM-1 but negatively related to <em>bla</em>_KPC-2, <em>bla</em>_KPC-3, <em>bla</em>_IMP and <em>bla</em>_OXA-181. Additionally, plasmids carrying Type IV-A system were predominantly the vehicles of <em>bla</em>_NDM-1 gene. Protospacer search revealed that Type IV-A system frequently matched conjugation transfer region of <em>bla</em>_KPC-2-related IncF plasmids, especially IncFIB(K)/IncFII(K), IncFII(pHN7A8)/IncR, and IncFIB(pQil)/IncFII(K) plasmids. The prevalence of self-targeting event further highlighted the interference mechanism of transcriptional repression proposed by Type IV system. Despite frequent targeting of IncF plasmids by Type IV-A system, different types of IncF plasmids displayed varying distribution between CRISPR-positive and -negative genomes, thereby suggesting a differentiated response of Type IV-A system to IncF plasmids. Our results underscore complex interactions between Type IV-A system and plasmid-mediated carbapenemase genes, revealing its significant role in shaping the transmission dynamics of carbapenemase-encoding plasmids.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128297"},"PeriodicalIF":6.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"There and back again: Genomic insights into microbial life in a recirculating petroleum refinery wastewater biotreatment system","authors":"Fengji Wu ,&nbsp;Bronwyn C. Campbell ,&nbsp;Paul Greenfield ,&nbsp;Grant C. Hose ,&nbsp;David J. Midgley ,&nbsp;Simon C. George","doi":"10.1016/j.micres.2025.128299","DOIUrl":"10.1016/j.micres.2025.128299","url":null,"abstract":"<div><div>Petroleum refinery wastewater biotreatment relies on microbes to remediate carbon, nitrogen, and sulfur compounds, yet their life strategies and ecological roles remain unclear. This study characterises the ecological functions of 20 metagenome-assembled genomes (MAGs) from a full-scale petroleum refinery wastewater treatment plant in southern China. The taxonomic identity, nutrient metabolism genes (including C/N/S cycling), carbohydrate-active enzymes, and CRISPR-Cas systems of these MAGs were analysed. The recovered MAGs represented bacteria primarily from the Pseudomonadota and Bacteroidota phyla. The major carbon sources for the represented organisms are likely aromatic and aliphatic compounds, as well as carbohydrates including peptidoglycan, chitin, and starch. Almost all MAGs contained genes for nitrate or nitrite reduction, while metabolic pathways for sulfur metabolism were generally less prevalent. <em>Meiothermus</em> sp. bin.89 was the most metabolically versatile MAG. This organism possessed genes that allowed it to recycle biomass, break down aliphatic and monoaromatic compounds, and perform anaerobic respiration using nitrate. However, it was likely the most susceptible to viral predation, as indicated by the high abundance of CRISPR spacers. Overall, the results revealed that stress-tolerant ecological traits were common among organisms in this microbiome, showcasing the ability of the microbes to obtain carbon from aromatic and aliphatic compounds. This study provides a substantial contribution towards future efforts in optimising microbiome stability for pollutant removal in petroleum refinery wastewater biotreatment systems.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128299"},"PeriodicalIF":6.9,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144826748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetylcholine chemotaxis in global bacterial plant pathogens","authors":"Jose A. Gavira ,&nbsp;Manuel J. Gilabert ,&nbsp;Saray Santamaría-Hernando ,&nbsp;Ana Molina-Ollero ,&nbsp;Miriam Rico-Jiménez ,&nbsp;Juan J. Cabrera ,&nbsp;Emilia López-Solanilla ,&nbsp;Miguel A. Matilla","doi":"10.1016/j.micres.2025.128294","DOIUrl":"10.1016/j.micres.2025.128294","url":null,"abstract":"<div><div>Interactions within the plant holobiont involve a wide diversity of plant- and microbial-derived signals. Bacterial perception of these signals allows directed movement along chemical gradients through chemotaxis, a process that is an important determinant for plant colonization and infection. The quaternary amine acetylcholine is primarily known for its function as a neurotransmitter in mammalian nervous systems, but is emerging as a signal molecule that regulates plant growth, development, and stress resistance. Here, we report that plant pathogenic bacteria of the genera <em>Agrobacterium</em> and <em>Dickeya</em> exhibit strong chemotaxis to acetylcholine. These responses are mediated in <em>Agrobacterium fabrum</em>, <em>Dickeya solani</em>, and <em>Dickeya dadantii</em> by the dCache_1-type ligand binding domain (LBD) containing chemoreceptors AtuA, MkcA, and DdaA, respectively, which recognize acetylcholine with affinities between 19 and 91 µM. These chemoreceptors also recognize additional quaternary amines such as choline, L-carnitine, and betaine. The high-resolution structure of MkcA-LBD of <em>D. solani</em> was solved in complex with choline, which allowed the identification of the molecular determinants of choline and acetylcholine recognition in this chemoreceptor. Functionally, DdaA was found to contribute to competitive colonization fitness <em>in planta</em>. Acetylcholine serves as a nutritional source for <em>A. fabrum,</em> but not for <em>D. solani</em> and <em>D. dadantii</em>, and provides osmoprotective effects in all three phytopathogenic species. Plant-associated bacteria exhibit chemotaxis toward a diversity of key plant signals, and their ability to sense acetylcholine may represent an adaptive strategy to ensure efficient plant infection and manage osmotic stress. Our findings suggest potential co-evolutionary interactions between plants and their associated microbiomes.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"300 ","pages":"Article 128294"},"PeriodicalIF":6.9,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importance of chick origin in introducing multidrug-resistant and extended-spectrum beta-lactamase-producing Enterobacteriaceae into an organic broiler farm","authors":"Anna Maria Korves ,&nbsp;Büsra Sardogan ,&nbsp;Kathrin Oelgeschläger ,&nbsp;Tanja Skladnikiewicz-Ziemer ,&nbsp;Frauke Umbach ,&nbsp;Margret Krieger ,&nbsp;Matthias Flor ,&nbsp;Mirjam Grobbel ,&nbsp;Annemarie Käsbohrer ,&nbsp;Bernd-Alois Tenhagen ,&nbsp;Ulrike Binsker","doi":"10.1016/j.micres.2025.128291","DOIUrl":"10.1016/j.micres.2025.128291","url":null,"abstract":"<div><div>Multidrug-resistant (MDR) and extended-spectrum beta-lactamase (ESBL)-producing <em>Enterobacteriaceae</em> occur in organic broiler production despite limited antimicrobial use. Their origin is not fully understood. This study characterized the genetic background and transmission dynamics of antimicrobial-resistant <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> from one German organic broiler farm throughout the fattening period. Four consecutive flocks were sampled five times each, from day-old chicks until slaughter age. Three isolates per bacterium were tested per sample using broth microdilution. In flocks 1–3 supplied by one hatchery, 37.8 % (17/45) of <em>E. coli</em> from non-selective medium showed MDR phenotypes from day-old chicks onwards, but no ESBL-producers were detected. Instead, MDR and ESBL-producing <em>K. pneumoniae</em> were isolated from selective medium in 73.3 % (11/15) of samples. Whole genome sequencing revealed clonal MDR and ESBL-producing <em>K. pneumoniae</em> harboring a conjugative IncFII/IncFIB resistance plasmid and belonging to ST307, an emerging high-risk clone in human medicine. MDR <em>E. coli</em> were genetically diverse, with distinct clusters of clonal MDR ST162 and ST57 <em>E. coli</em>. In flock 4, supplied by a different hatchery, no MDR or ESBL-producing <em>E. coli</em> and <em>K. pneumoniae</em> were detected. 66.7 % of <em>E. coli</em> and 100 % of <em>K. pneumoniae</em> were fully susceptible. These results demonstrate the impact of external sources, particularly chick origin, on introducing of resistant bacteria into organic broiler farms. Further research is needed to assess the prevalence and the genetic background of MDR and ESBL-producing <em>Enterobacteriaceae</em> in organic breeder flocks and hatcheries to identify potential transmission events and improve prevention strategies.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128291"},"PeriodicalIF":6.9,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}