Microbiological research最新文献

{"title":"Exploring the multifaceted role of pehR in Ralstonia solanacearum pathogenesis: enzyme activity, motility, and biofilm formation","authors":"Sharmilee Sarkar ,&nbsp;Mohit Yadav ,&nbsp;Upalabdha Dey,&nbsp;Manoj Sharma ,&nbsp;Rupak Mukhopadhyay ,&nbsp;Aditya Kumar","doi":"10.1016/j.micres.2024.127925","DOIUrl":"10.1016/j.micres.2024.127925","url":null,"abstract":"<div><div>PehR is a transcriptional regulator among the various response regulators found in <em>Ralstonia solanacearum</em>, a bacterium that causes lethal wilt disease in over 450 plant species worldwide, including economically important crops such as tomato, chilli, and brinjal. PehR regulates the production of polygalacturonase, an extracellular enzyme that degrades plant cell walls, playing a significant role in bacterial wilt. Despite its significance, the precise function and regulatory mechanism of PehR in <em>R. solanacearum</em> are yet to be thoroughly investigated. The goal of this research is to better understand the role of PehR in <em>R. solanacearum</em> pathogenicity by identifying the genes and pathways that it regulates. By disrupting the <em>pehR</em> gene, we created the <em>ΔpehR</em> mutant of <em>R. solanacearum</em> F1C1, a strain isolated from Tezpur, Assam, India. Transcriptomic analysis revealed 667 differentially expressed genes (DEGs) in the <em>ΔpehR</em> mutant, with 320 upregulated and 347 downregulated compared to the wild-type F1C1 strain. GO and KEGG analyses indicated the downregulation of genes related to flagellum-dependent cell motility, membrane function, and amino acid degradation pathways in the <em>ΔpehR</em> mutant. EPS estimation, biochemical assays for biofilm production, motility, and enzymatic assays for cellulase and pectinase production were all used in the further characterization process. The <em>ΔpehR</em> mutant showed lower virulence in tomato seedlings compared to the wild-type F1C1 strain. The findings suggest that PehR could be a promising target for bacterial wilt disease control, as well as provide critical information for ensuring crop production safety around the world.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"290 ","pages":"Article 127925"},"PeriodicalIF":6.1,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression, regulation and physiological roles of the five Rsm proteins in Pseudomonas syringae pv. tomato DC3000","authors":"Adriana Vásquez ,&nbsp;María-Dolores Ferreiro ,&nbsp;Laura Martínez-Rodríguez,&nbsp;María-Trinidad Gallegos","doi":"10.1016/j.micres.2024.127926","DOIUrl":"10.1016/j.micres.2024.127926","url":null,"abstract":"<div><div>Proteins belonging to the RsmA (regulator of secondary metabolism)/CsrA (carbon storage regulator) family are small RNA-binding proteins that play crucial roles post-transcriptionally regulating gene expression in many Gram-negative and some Gram-positive bacteria. Although most of the bacteria studied have a single RsmA/CsrA gene, <em>Pseudomonas syringae</em> pv. tomato (Pto) DC3000 encodes five Rsm proteins: RsmA/CsrA2, RsmC/CsrA1, RsmD/CsrA4, RsmE/CsrA3, and RsmH/CsrA5. This work aims to provide a comprehensive analysis of the expression of these five <em>rsm</em> protein-encoding genes, elucidate the regulatory mechanisms governing their expression, as well as the physiological relevance of each variant. To achieve this, we examined the expression of <em>rsmA, rsmE, rsmC, rsmD, and rsmH</em> within their genetic contexts, identified their promoter regions, and assessed the impact of both their deletion and overexpression on various Pto DC3000 phenotypes. A novel finding is that <em>rsmA</em> and <em>rsmC</em> are part of an operon with the upstream genes, whereas <em>rsmH</em> seems to be co-transcribed with two downstream genes. We also observed significant variability in expression levels and RpoS dependence among the five <em>rsm</em> paralogs. Thus, despite the extensive repertoire of <em>rsm</em> genes in Pto DC3000, only <em>rsmA</em>, <em>rsmE</em> and <em>rsmH</em> were significantly expressed under all tested conditions (swarming, minimal and T3SS-inducing liquid media). Among these, RsmE and RsmA were corroborated as the most important paralogs at the functional level, whereas RsmH played a minor role in regulating free life and plant-associated phenotypes. Conversely, RsmC and RsmD did not seem to be functional under the conditions tested.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127926"},"PeriodicalIF":6.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging concepts in mucosal immunity and oral microecological control of respiratory virus infection-related inflammatory diseases","authors":"Ying Wang ,&nbsp;Jiaxuan Li ,&nbsp;Ruyi Chen ,&nbsp;Qiuyi Xu ,&nbsp;Di Wang ,&nbsp;Chenxi Mao ,&nbsp;Ziyi Xiang ,&nbsp;Guangshang Wu ,&nbsp;Ying Yu ,&nbsp;Jianhua Li ,&nbsp;Yuejuan Zheng ,&nbsp;Keda Chen","doi":"10.1016/j.micres.2024.127930","DOIUrl":"10.1016/j.micres.2024.127930","url":null,"abstract":"<div><div>Oral microecological imbalance is closely linked to oral mucosal inflammation and is implicated in the development of both local and systemic diseases, including those caused by viral infections. This review examines the critical role of the interleukin (IL)-17/helper T cell 17 (Th17) axis in regulating immune responses within the oral mucosa, focusing on both its protective and pathogenic roles during inflammation. We specifically highlight how the IL-17/Th17 pathway contributes to dysregulated inflammation in the context of respiratory viral infections. Furthermore, this review explores the potential interactions between respiratory viruses and the oral microbiota, emphasizing how alterations in the oral microbiome and increased production of proinflammatory factors may serve as early, non-invasive biomarkers for predicting the severity of respiratory viral infections. These findings provide insights into novel diagnostic approaches and therapeutic strategies aimed at mitigating respiratory disease severity through monitoring and modulating the oral microbiome.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127930"},"PeriodicalIF":6.1,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From probiotic chassis to modification strategies, control and improvement of genetically engineered probiotics for inflammatory bowel disease","authors":"Yuewen Huang ,&nbsp;Shan Peng ,&nbsp;Rong Zeng ,&nbsp;Hao Yao ,&nbsp;Guangfu Feng ,&nbsp;Jun Fang","doi":"10.1016/j.micres.2024.127928","DOIUrl":"10.1016/j.micres.2024.127928","url":null,"abstract":"<div><div>With the rising morbidity of inflammatory bowel disease (IBD) year by year, conventional therapeutic drugs with systemic side effects are no longer able to meet the requirements of patients. Probiotics can improve gut microbiota, enhance intestinal barrier function, and regulate mucosal immunity, making them a potential complementary or alternative therapy for IBD. To compensate for the low potency of probiotics, genetic engineering technology has been widely used to improve their therapeutic function. In this review, we systematically summarize the genetically engineered probiotics used for IBD treatment, including probiotic chassis, genetic modification strategies, methods for controlling probiotics, and means of improving efficacy. Finally, we provide prospects on how genetically engineered probiotics can be extended to clinical applications.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127928"},"PeriodicalIF":6.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics reveals the regulation of the immune system of the mushroom-forming fungus Schizophyllum commune during interaction with four competitors","authors":"Erik P.W. Beijen,&nbsp;Marieke H. van Maanen,&nbsp;Ioana M. Marian,&nbsp;Janieke X. Klusener,&nbsp;Emmeline van Roosmalen,&nbsp;Koen C. Herman,&nbsp;Margot C. Koster,&nbsp;Robin A. Ohm","doi":"10.1016/j.micres.2024.127929","DOIUrl":"10.1016/j.micres.2024.127929","url":null,"abstract":"<div><div>Mushroom-forming fungi frequently encounter competitors during their lifecycle, but their defense mechanisms remain largely unexplored. We studied the response of the mushroom-forming fungus <em>Schizophyllum commune</em> during interaction with the fungal competitors <em>Trichoderma harzianum</em>, <em>Trichoderma aggressivum</em> and <em>Purpureocillium lilacinum</em> and the bacterial competitor <em>Serratia quinivorans.</em> Transcriptomics revealed 632 up-regulated genes in the direct interaction zone, which were enriched in small secreted proteins and transporters. A set of 26 genes were up-regulated during all interactions, indicating a core transcriptomic defense response. In the non-interacting edge of the mycelium of <em>S. commune</em>, there were 154 up-regulated genes, suggesting that there is a systemic response due to a signal that reaches unaffected areas. The GATA zinc finger transcription factor gene <em>gat1</em> was up-regulated during interaction and a Δ<em>gat1</em> strain displayed increased colonization by <em>T. harzianum</em>. Previously linked to mushroom development, this transcription factor apparently has a dual role. Moreover, 138 genes were up-regulated during both interaction and mushroom development, indicating priming of the defense response during development to prepare the fruiting body for future interactions. Overall, we unveiled a defensive response of <em>S. commune</em> during interaction with fungal and bacterial competitors and identified a regulator of this response.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127929"},"PeriodicalIF":6.1,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Christensenellaceae minuta modulates epithelial healing via PI3K-AKT pathway and macrophage differentiation in the colitis","authors":"Ting Yao ,&nbsp;Youhe Wu ,&nbsp;Liyun Fu,&nbsp;Jiawen Lv,&nbsp;Longxian Lv,&nbsp;Lanjuan Li","doi":"10.1016/j.micres.2024.127927","DOIUrl":"10.1016/j.micres.2024.127927","url":null,"abstract":"<div><div>Ulcerative colitis (UC) is a chronic inflammatory disorder with an unsatisfactory cure rate and mucosal healing is a key treatment objective. <em>Christensenellaceae minuta</em> (<em>C. minuta</em>) has emerged as a next-generation of probiotic for maintaining intestinal health. We investigated the therapeutic efficacy of <em>C. minuta</em> in dextran sulfate sodium (DSS)-induced colitis, focusing on mucosal healing and the underlying mechanisms. <em>C. minuta</em> effectively alleviated colitis and promoted the regeneration of intestinal epithelial cells (IECs). Using 16S rRNA sequencing and metabolomics, we found that <em>C. minuta</em> administration increased beneficial bacteria, decreased pathogenic bacteria, and significantly elevated propionic acid levels. Additionally<em>, C. minuta</em> activated the PI3K-AKT pathway by upregulating systemic and local IGF-1 expression. Inhibiting the PI3K-AKT pathway reduced the therapeutic effects of <em>C. minuta</em> and impaired IEC regeneration. Furthermore, <em>C. minuta</em> promoted macrophage differentiation into the M2 phenotype and decreased proinflammatory factors. We propose that <em>C. minuta</em> alleviates colitis by regulating the gut microbiota, modulating macrophage differentiation, and enhancing mucosal healing by activating the PI3K-AKT pathway via IGF-1 secretion induced by short-chain fatty acids. Our findings provide evidence from animal experiments to support future clinical trials and the therapeutic translation of <em>C. minuta</em>.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127927"},"PeriodicalIF":6.1,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brassinosteroids mediate arbuscular mycorrhizal symbiosis through multiple potential pathways and partial identification in tomato","authors":"Ying Ren ,&nbsp;Brian Tobin ,&nbsp;Shuyi Yang ,&nbsp;Tingying Xu ,&nbsp;Hui Chen ,&nbsp;Ming Tang","doi":"10.1016/j.micres.2024.127924","DOIUrl":"10.1016/j.micres.2024.127924","url":null,"abstract":"<div><div>Currently, little is known regarding the specific processes through which brassinosteroids (BR) affect arbuscular mycorrhizal (AM) symbiosis. Understanding this relationship is vital for advancing plant physiology and agricultural applications. In this study, we aimed to elucidate the regulatory mechanisms of BR in AM symbiosis. According to the log2 fold change-value and adjP-value, we integrated the common differentially expressed genes (DEGs) in maize (<em>Zea mays</em> L.) treated with BR and AM, Arabidopsis (<em>Arabidopsis thaliana</em>) mutants deficient in BR receptors, and tomato (<em>Solanum lycopersicum</em>) plants inoculated with AM fungi. In addition, we characterized the symbiotic performance of tomato plants with BR receptor defects and overexpression. The results indicated that the common differential genes induced by BR and AM were involved in metabolic processes, such as cell wall modification, cytoskeleton remodeling, auxin and ethylene signaling, photosynthesis, mineral nutrient transport, and stress defense. Specifically, these include the <em>BR1</em> gene, which modifies the cell wall. However, the fungal colonization rate of BR receptor-deficient tomato plants was significantly reduced, and the total phosphorus concentration was increased. Conversely, the performance of the overexpressing tomato transformation plants demonstrated a significant contrast. Additionally, the mild rescue of mycorrhizal attenuation in mutants treated with exogenous BR suggests the possibility of direct feedback from BR synthesis to AM. Notably, the cell wall modification gene (<em>SlBR1</em>) and calcium spike gene (<em>SlIPD3</em>) were induced by both BR and AM, suggesting that BR may influence cell penetration during the early stages of AM colonization. Synthesis: Our results demonstrated that BR positively regulates AM symbiosis through multiple pathways. These findings pave the way for future research, including isolation of the individual contributions of each pathway to this complex process and exploration of possible agricultural applications.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127924"},"PeriodicalIF":6.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemoreceptor MCP4580 of Vibrio splendidus mediates chemotaxis toward L-glutamic acid contributing to bacterial virulence","authors":"Ya Li ,&nbsp;Weibo Shi ,&nbsp;Zihao Sun ,&nbsp;Weiwei Zhang","doi":"10.1016/j.micres.2024.127917","DOIUrl":"10.1016/j.micres.2024.127917","url":null,"abstract":"<div><div>Chemotaxis has an essential function in flagellar bacteria that allows them to sense and respond to specific environmental signals, enabling their survival and colonization. <em>Vibrio splendidus</em> is an important opportunistic pathogen that infects a wide range of hosts including fish, bivalve, and sea cucumber<em>.</em> Our study demonstrated that <em>V. splendidus</em> AJ01 exhibited chemotaxis toward L-glutamic acid (L-Glu), an abundant amino acid in the intestinal and respiratory tree tissues of the sea cucumber. Bacterial samples collected from two locations in soft agar swimming plates were subjected to RNA-sequencing (RNA-Seq) analysis to identify the methyl-accepting chemotaxis protein (MCP) respond to L-Glu. Among the 40 annotated chemoreceptors, MCP4580 was identified as the MCP that mediates L-Glu-response. Molecular docking and site-directed mutagenesis revealed that L-arginine at residue 81 (R81) and L-glutamine at residue 88 (Q88) in the ligand-binding domain (LBD) are crucial for L-Glu recognition. Bacterial two-hybrid assay (BTH) showed that MCP4580 forms dimers and interacts with the histidine kinase CheA via the coupling protein CheW1 and CheW2. Phosphorylation analysis showed that the binding of L-Glu to MCP4580 results in the inhibition of CheA phosphorylation mainly via CheW1. Notably, sea cucumbers stimulated with each mutant strain of chemotaxis protein exhibited reduced mortality, highlighting the importance of chemotaxis in <em>V. splendidus</em> virulence. The present study provides valuable insights into the molecular components and signal transduction involved in the chemotaxis of <em>V. splendidus</em> toward L-Glu, and highlights the importance of chemotaxis in its virulence.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127917"},"PeriodicalIF":6.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The global transcription factor Clp exerts positive regulatory effects in the walnut bacterial black spot pathogen, Xanthomonas arboricola pv. juglandis","authors":"Feng Li ,&nbsp;Wenzhong Yang ,&nbsp;Benzhong Fu ,&nbsp;Yonghong Yu ,&nbsp;Yahui Mao","doi":"10.1016/j.micres.2024.127921","DOIUrl":"10.1016/j.micres.2024.127921","url":null,"abstract":"<div><div>Walnut blight caused by the bacterium <em>Xanthomonas arboricola</em> pv. <em>juglandis</em> (<em>Xaj</em>) is one of the most common diseases of walnut (<em>Juglans</em> spp.), resulting in serious yield decline and significant economic losses. Crp-like protein (Clp) is an important global regulatory transcription factor in bacteria. In this study, we sought to elucidate the role of Clp in the pathogenicity of <em>Xaj</em> strain DW3F3 and the associated regulatory mechanism. The results indicated that <em>clp</em> gene deficiency significantly reduced the pathogenicity of <em>Xaj</em> DW3F3 in walnut without affecting the growth of the bacterium. We found that Clp positively regulates biofilm formation, extracellular polysaccharide production, exoenzyme secretion, and motility of <em>Xaj</em>, which was consistent with the transcript levels of virulence factor-encoding genes. However, overexpression of <em>clp</em> does not enhance the expression of all virulence genes, it may inhibit the expression of a part of virulence factor-related genes. EMSA assay further showed that Clp specifically binds to the promoters of these genes and regulates their expression, and CD spectra test certified that the ligand of Clp was c-di-GMP. Our findings contribute to the in-depth understanding of the pathogenic mechanism of <em>Xaj</em> and highlight the potential of Clp as a drug target for the development of agents to prevent and control walnut diseases.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127921"},"PeriodicalIF":6.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sterol regulatory element-binding proteins mediate intrinsic fungicide tolerance and antagonism in the fungal biocontrol agent Clonostachys rosea IK726","authors":"Edoardo Piombo ,&nbsp;Georgios Tzelepis ,&nbsp;Alma Gustavsson Ruus,&nbsp;Vahideh Rafiei,&nbsp;Dan Funck Jensen,&nbsp;Magnus Karlsson,&nbsp;Mukesh Dubey","doi":"10.1016/j.micres.2024.127922","DOIUrl":"10.1016/j.micres.2024.127922","url":null,"abstract":"<div><div>Sterol regulatory element-binding proteins (SREBPs) are transcription factors governing various biological processes in fungi, including virulence and fungicide tolerance, by regulating ergosterol biosynthesis and homeostasis. While studied in model fungal species, their role in fungal species used for biocontrol remains elusive. This study delves into the biological and regulatory function of SREBPs in the fungal biocontrol agent (BCA) <em>Clonostachys rosea</em> IK726, with a specific focus on fungicide tolerance and antagonism<em>. Clonostachys rosea</em> genome contains two SREBP coding genes (<em>sre1</em> and <em>sre2</em>) with distinct characteristics. Deletion of <em>sre1</em> resulted in mutant strains with pleiotropic phenotypes, including reduced <em>C. rosea</em> growth on medium supplemented with prothioconazole and boscalid fungicides, hypoxia mimicking agent CoCl₂ and cell wall stressor SDS, and altered antagonistic abilities against <em>Botrytis cinerea</em> and <em>Rhizoctonia solani</em>. However, Δ<em>sre2</em> strains showed no significant effect. Consistent with the gene deletion results, overexpression of <em>sre1</em> in <em>Saccharomyces cerevisiae</em> enhanced tolerance to prothioconazole. The functional differentiation between SRE1 and SRE2 was elucidated by the yeast-two-hybridization assay, which showed an interaction between SREBP cleavage-activating protein (SCAP) and SRE1 but not between SRE2 and SCAP. Transcriptome analysis of the Δ<em>sre1</em> strain unveiled SRE1-mediated expression regulation of genes involved in lipid metabolism, respiration, and xenobiotic tolerance. Notably, genes coding for antimicrobial compounds chitinases and polyketide synthases were downregulated, aligning with the altered antagonism phenotype. This study uncovers the role of SREBPs in fungal BCAs, providing insights for <em>C. rosea</em> IK726 application into integrated pest management strategies.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"289 ","pages":"Article 127922"},"PeriodicalIF":6.1,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}