Integrated microbiome and metabolome approaches reveal the regulatory mechanism of pumpkin pollination

IF 6.9 1区 生物学 Q1 MICROBIOLOGY
Jian Xiao , Yu Zhu , Liyuan Liao , Baoling Chen , Wenjun Liu , Shangdong Yang
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Abstract

The fruit set in the Cucurbitaceae family is a critical determinant of fruit production and development. However, limited information is available regarding the regulatory mechanisms relating to pumpkin fruit sets. To elucidate the interplay between pumpkin fruit setting and endophytic microorganisms, we conducted a comparative analysis of the endophytic microbiota and metabolite profiles in the stems of naturally pollinated and non-pollinated pumpkin using microbiome and untargeted metabolomics approaches. The results showed that both the alpha- (reduced by 18.33∼21.88 % and 16.63∼24.08 %) and beta-diversities (reduced by 12.40 % and 40.00 %) of endophytic microorganisms (bacteria and fungi) in stems of pollinated pumpkins were significantly reduced which were significantly reduced compared to those in non-pollinated pumpkins. Meanwhile, in comparison with pollinated pumpkins, the deficiency of endophytic bacterial genera that regulate endogenous hormones and metabolites, such as Pantoea, Staphylococcus, Brevundimonas, Tatumella, and Gluconobacter, and the weak metabolic pathways, viz, the relatively stable homeostasis, such as flavone and flavonol biosynthesis, alanine, aspartate, and glutamate metabolism, and phenylpropanoid biosynthesis in stems of non-pollinated pumpkin were important reasons why fruits could not bear fruits without pollination. All above results reveal that endophytic microorganisms are closely related to the growth and development of pumpkins, also, the endophytic microbial community structures in stems of pumpkins can be reshaped by man-made measures, such as pollination.
微生物组学和代谢组学的综合研究揭示了南瓜授粉的调控机制
在葫芦科植物中,坐果是决定果实生产和发育的关键因素。然而,关于南瓜结实的调控机制的信息有限。为了阐明南瓜坐果与内生微生物之间的相互作用,我们利用微生物组学和非靶向代谢组学方法对自然授粉和非授粉南瓜茎中的内生微生物群和代谢物进行了比较分析。结果表明,与未授粉南瓜相比,授粉南瓜茎内内生微生物(细菌和真菌)α - (α -减少18.33 ~ 21.88 %和16.63 ~ 24.08 %)和β -多样性(α -减少12.40 %和40.00 %)均显著降低。同时,与授粉南瓜相比,缺乏调节内源激素和代谢物的内生细菌属,如Pantoea、Staphylococcus、Brevundimonas、Tatumella和Gluconobacter,代谢途径较弱,即相对稳定的内稳态,如黄酮和黄酮醇生物合成,丙氨酸、天冬氨酸和谷氨酸代谢。不授粉南瓜茎中苯丙素的合成是不授粉南瓜果实不结实的重要原因。上述结果表明,内生微生物与南瓜的生长发育密切相关,并且可以通过授粉等人为措施重塑南瓜茎部的内生微生物群落结构。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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