Microbiological research最新文献

{"title":"Revisiting therapeutic options against resistant klebsiella pneumoniae infection: Phage therapy is key","authors":"Jiabao Xing ,&nbsp;Rongjia Han ,&nbsp;Jinxin Zhao ,&nbsp;Yuying Zhang ,&nbsp;Meng Zhang ,&nbsp;Yichao Zhang ,&nbsp;Hang Zhang ,&nbsp;Sue C. Nang ,&nbsp;Yajun Zhai ,&nbsp;Li Yuan ,&nbsp;Shanmei Wang ,&nbsp;Hua Wu","doi":"10.1016/j.micres.2025.128083","DOIUrl":"10.1016/j.micres.2025.128083","url":null,"abstract":"<div><div>Multi-drug resistant and carbapenem-resistant hypervirulent <em>Klebsiella pneumoniae</em> strains are spreading globally at an alarming rate, emerging as one of the most serious threats to global public health. The formidable challenges posed by the current arsenal of antimicrobials highlight the urgent need for novel strategies to combat <em>K. pneumoniae</em> infections. This review begins with a comprehensive analysis of the global dissemination of virulence factors and critical resistance profiles in <em>K. pneumoniae</em>, followed by an evaluation of the accessibility of novel therapeutic approaches for treating <em>K. pneumoniae</em> in clinical settings. Among these, phage therapy stands out for its considerable potential in addressing life-threatening <em>K. pneumoniae</em> infections. We critically examine the existing preclinical and clinical evidence supporting phage therapy, identifying key limitations that impede its broader clinical adoption. Additionally, we rigorously explore the role of genetic engineering in expanding the host range of <em>K. pneumoniae</em> phages, and discuss the future trajectory of this technology. In light of the 'Bad Bugs, No Drugs' era, we advocate leveraging artificial intelligence and deep learning to optimize and expand the application of phage therapy, representing a crucial advancement in the fight against the escalating threat of <em>K. pneumoniae</em> infections.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128083"},"PeriodicalIF":6.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 1-acylglycerol-3-phosphate acyltransferase Slc1 is required to regulate mitochondria and lipid droplets","authors":"Chenhui Zhao ,&nbsp;Ke Liu ,&nbsp;Yifan Wu ,&nbsp;Shuaijie Yan ,&nbsp;Jiajia He ,&nbsp;Chuanhai Fu","doi":"10.1016/j.micres.2025.128080","DOIUrl":"10.1016/j.micres.2025.128080","url":null,"abstract":"<div><div>Mitochondria are organelles involved in energy metabolism and biosynthesis. As the metabolites released from mitochondria are raw materials used for lipid synthesis, mitochondria also play important roles in lipid metabolism. Here we report that Slc1, a 1-acylglycerol-3-phosphate O-acyltransferase in the fission yeast <em>Schizosaccharomyces pombe</em>, is required to maintain tubular mitochondrial morphology and normal mitochondrial functions. The absence of Slc1 causes mitochondrial fragmentation, increases mitochondrial fission frequency, reduces mitochondrial respiration, and slows down nitrogen starvation-induced mitophagy. In addition, the absence of Slc1 significantly increases the protein level of Ptl2, which is the triacylglycerol lipase localized on lipid droplets. The phenotypes caused by the absence of Slc1 depend on its acyltransferase enzymatic activity. Therefore, our study uncovers new roles of a lipid synthesis enzyme Slc1 in regulating mitochondria and lipid droplets.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128080"},"PeriodicalIF":6.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-inoculation of Trichoderma and tea root-associated bacteria enhance flavonoid production and abundance of mycorrhizal colonization in tea (Camellia sinensis)","authors":"Anupam Mondal ,&nbsp;Sk Soyal Parvez ,&nbsp;Anusha Majumder ,&nbsp;Kalpna Sharma ,&nbsp;Bimal Das ,&nbsp;Utpal Bakshi ,&nbsp;Masrure Alam ,&nbsp;Avishek Banik","doi":"10.1016/j.micres.2025.128084","DOIUrl":"10.1016/j.micres.2025.128084","url":null,"abstract":"<div><div>Tea is one of the most popular nonalcoholic beverages, that contains several medicinally important flavonoids. Due to seasonal variation and various environmental stresses, the overall consistency of tea flavonoids affects the tea quality. To combat stress, plants stimulate symbiotic relationships with root-associated beneficial microbiomes that sustain nutrient allocation. Therefore, a study has been designed to understand the role of the tea root microbiome in sustaining tea leaf flavonoid production. To enumerate the microbiome, tea root and rhizoplane soil were collected from 3 years of healthy plants from Jalpaiguri district, West Bengal, India. A culture-independent approach was adopted to identify root and rhizosphere microbial diversity (BioSample: SAMN31404869; SRA: SRS15503027 [rhizosphere soil metagenome] BioSample: SAMN31404868;SRA:SRS15503030 [root metagenome]. In addition to diverse microbes, four mycorrhiza fungi, i.e., <em>Glomus intraradices</em>, <em>Glomus irregulare, Paraglomus occultum</em> and <em>Scutellospora heterogama</em> were predominant in collected root samples. A culture-dependent approach was also adopted to isolate several plant growth-promoting bacteria [<em>Bacillus</em> sp. D56, <em>Bacillus</em> sp. D42, <em>Bacillus</em> sp. DR15, <em>Rhizobium</em> sp. DR23 (NCBI Accession: OR821747-OR821750)] and one fungal [<em>Trichoderma</em> sp<em>.</em> AM6 (NCBI Accession:OM915414)] strain. A pot experiment was designed to assess the impact of that isolated microbiome on tea seedlings. After six months of microbiome inoculation, tea plants' physicochemical and transcriptional parameters were evaluated. The results confer that the microbiome-treated treatments [(T1-without any microbial inoculation; NCBI Accession: SAMN33591153), <em>Trichoderma</em> sp. AM6 (T2; NCBI Accession: SAMN33591155) and <em>Trichoderma</em> sp. AM6 +VAM containing tea root+synthetic microbial consortia (T5; NCBI Accession: SAMN33591154)] could enhance the total flavonoid content in tea seedlings by upregulating certain transcripts associated with the flavonoid biosynthesis pathway of tea.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128084"},"PeriodicalIF":6.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-free synthesis system: An accessible platform from biosensing to biomanufacturing","authors":"Yongbin Chen ,&nbsp;Wenhao Xia ,&nbsp;Fuping Lu ,&nbsp;Zhen Chen ,&nbsp;Yihan Liu ,&nbsp;Mingfeng Cao ,&nbsp;Ning He","doi":"10.1016/j.micres.2025.128079","DOIUrl":"10.1016/j.micres.2025.128079","url":null,"abstract":"<div><div>The fundamental aspect of cell-free synthesis systems is the <em>in vitro</em> transcription-translation process. By artificially providing the components required for protein expression, <em>in vitro</em> protein production alleviates various limitations tied to <em>in vivo</em> production, such as oxygen supply and nutrient constraints, thus showcasing substantial potential in engineering applications. This article presents a comprehensive review of cell-free synthesis systems, with a primary focus on biosensing and biomanufacturing. In terms of biosensing, it summarizes the recognition-response mechanisms and key advantages of cell-free biosensors. Moreover, it examines the strategies for the cell-free production of intricate proteins, including membrane proteins and glycoproteins. Additionally, the integration of cell-free metabolic engineering approaches with cell-free synthesis systems in biomanufacturing is thoroughly discussed, with the expectation that biotechnology will embrace greater prosperity.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128079"},"PeriodicalIF":6.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and metabolomic insights into the modes-of-action of bacterial strains to control the grapevine wood pathogen, Fomitiporia mediterranea","authors":"Ouiza Mesguida ,&nbsp;Stéphane Compant ,&nbsp;Adrian Wallner ,&nbsp;Livio Antonielli ,&nbsp;Ryszard Lobinski ,&nbsp;Simon Godin ,&nbsp;Mickaël Le Bechec ,&nbsp;Maxence Terrasse ,&nbsp;Ahmed Taibi ,&nbsp;Assia Dreux-Zigha ,&nbsp;Jean-Yves Berthon ,&nbsp;Rémy Guyoneaud ,&nbsp;Patrice Rey ,&nbsp;Eléonore Attard","doi":"10.1016/j.micres.2025.128085","DOIUrl":"10.1016/j.micres.2025.128085","url":null,"abstract":"<div><div>Grapevine trunk diseases (GTDs), particularly Esca, represent a major challenge for viticulture worldwide, leading to substantial economic losses. With no effective control treatments available, developing new methods such as biocontrol is crucial for managing GTDs. Our aim was to select biocontrol bacteria effective against the white-rot fungal pathogen <em>Fomitiporia mediterranea</em> (Fmed) and to investigate their mechanisms of action. A stepwise screening of 58 bacterial strains was conducted <em>in vitro</em> to assess their ability to inhibit Fmed growth through volatile and diffusible metabolites production. The screening was also done on wood sawdust from seven different grapevine cultivars. Out of 58 tested strains, 49 inhibited Fmed growth by over 50 % through their volatile organic compounds, only eight achieving this through their agar-diffusible metabolites. <em>Pseudomonas lactis</em> SV9, <em>Pseudomonas paracarnis</em> S45, and <em>Paenibacillus polymyxa</em> SV13 exhibited a strong efficacy in inhibiting Fmed on wood sawdust in a cultivar-dependent manner. We selected these strains for whole genome analysis and metabolomic profiling via LC-MS/MS for diffusible compounds and SPME GC-MS for volatile compounds. <em>P. polymyxa</em> SV13 inhibited Fmed primarily through diffusible metabolites, producing mainly fusaricidin-type compounds. Conversely, <em>Pseudomonas</em> strains acted mainly via their volatile metabolites, producing mainly the antifungal compound dimethyl disulfide. Genome analysis of the three bacterial strains revealed gene clusters responsible for regulating both direct and indirect mechanisms in biocontrol agents (BCAs). Our findings highlight the importance of comprehensive studies that combine <em>in vitro</em> experiments mimicking field conditions, with detailed investigations into modes of action to improve BCAs efficacy.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128085"},"PeriodicalIF":6.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of novel Erwinia amylovora-specific phiEaSP1 phage and its application as phage cocktail for managing fire blight in apples","authors":"Hyeongsoon Kim ,&nbsp;Nguyen Trung Vu ,&nbsp;Roniya Thapa Magar ,&nbsp;Eom-Ji Oh ,&nbsp;Ki-Hoon Oh ,&nbsp;Jiwon Lee ,&nbsp;LeeSeul Kim ,&nbsp;In Sun Hwang ,&nbsp;Chang-Sik Oh","doi":"10.1016/j.micres.2025.128087","DOIUrl":"10.1016/j.micres.2025.128087","url":null,"abstract":"<div><div><em>Erwinia amylovora</em> (Ea) is a devastating bacterial pathogen that causes fire blight disease in <em>Rosaceae</em> family plants, including apples and pears. The use of bacteriophages is an alternative strategy to antibiotics for managing bacterial pathogens. In this study, the Ea-specific virulent phiEaSP1 phage was characterized, and its biocontrol efficacy against Ea was evaluated in apple seedlings. Genomic analyses revealed that phiEaSP1 belongs to the family <em>Chaseviridae</em>, subfamily <em>Cleopatravirinae</em>, and genus <em>Loessnervirus</em>. Most phiEaSP1 particles bound to the host cell surface within 5 min, and one virion made 68 progenies within 20 min of infection. The phage rapidly lysed Ea cells <em>in vitro</em> and maintained its lytic activity after incubation under different environmental conditions, including temperature, pH, and UV-A, as well as in the soil, with surfactants, and on apple seedlings. Receptor analysis using the Tn5 random mutant library of Ea TS3128 demonstrated that phiEaSP1 recognizes lipopolysaccharide as a receptor, whereas phiEaP-8 and phiEaP-21 recognize cellulose as a receptor. Protective efficacy against fire blight was tested on apple seedlings pretreated with the single phiEaSP1 or a phage cocktail containing phiEaSP1, phiEaP-8, and phiEaP-21. No or only weak symptoms were observed in the phage-treated seedlings. The application of a phage cocktail showed better control efficacy, indicating the potential of the phage cocktail, including phiEaSP1, as a preventive agent. Taken together, these results suggest that the use of a phage cocktail containing phiEaSP1 could be a potential strategy for the biocontrol of fire blight disease in apples.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128087"},"PeriodicalIF":6.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keystone root bacteria in Ambrosia artemisiifolia promote invasive growth by increasing the colonization rate of Funneliformis mosseae","authors":"Chunying Li ,&nbsp;Xue Chen ,&nbsp;Jieyu Yang ,&nbsp;Jun Li ,&nbsp;Ruiyue Wang ,&nbsp;Haiyun Xu ,&nbsp;Fengjuan Zhang","doi":"10.1016/j.micres.2025.128081","DOIUrl":"10.1016/j.micres.2025.128081","url":null,"abstract":"<div><div>Higher arbuscular mycorrhizal fungi (AMF) colonization rates in the roots of invasive plants than in those of native plants are associated with invasion success. Keystone plant-root bacteria (or root-associated bacteria) can influence plant growth by interacting with other members of the microbial community (eg.AMF). We aimed to investigate the effects of keystone taxa on AMF colonization and their interactions on invasive plant growth. Here, the common key root-associated species from the roots of <em>Ambrosia artemisiifolia</em> among four geographical populations in China were identified, and the strains were subsequently isolated. Plate and pot experiments were conducted to examine the impact of keystone species on the colonization of <em>Funneliformis mosseae</em> and elucidate the mechanisms that enhance plant growth. <em>Sphingomonas</em> was identified as a common keystone root-associated genus of <em>A. artemisiifolia</em>. <em>Sphingomonas sanxanigenens</em> was found to facilitate AMF colonization in the roots of <em>A. artemisiifolia</em> by promoting flavonoid biosynthesis. A synergistic effect on the growth of <em>A. artemisiifolia</em> was observed when the plant was co-inoculated with <em>S. sanxanigenens</em> and <em>F. mosseae</em>. This study provides new insights into the mechanisms whereby root-associated microbes facilitate AMF colonization in invasive plants. These findings confirm the pivotal role of keystone microbes in weed invasion and enhance our understanding that microbial synergistic interactions promote weed invasiveness.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128081"},"PeriodicalIF":6.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of an E. coli flagellotrophic Jumbophage SHEFM2K that replicates in cytoplasmic putative assembly areas","authors":"Ghadah Alsharif ,&nbsp;Trong Khoa Pham ,&nbsp;Alexandra N. Connolly ,&nbsp;Karolina I. Pyrszanowska ,&nbsp;Elspeth M. Smith ,&nbsp;Alhassan Alrafaie ,&nbsp;Carl Smythe ,&nbsp;Graham P. Stafford","doi":"10.1016/j.micres.2025.128082","DOIUrl":"10.1016/j.micres.2025.128082","url":null,"abstract":"<div><div>The bacteriophage SHEFM2K was isolated from unpasteurised dairy farm milk using a newly isolated <em>E. coli</em> ExPEC strain EcM2K (O23:H8, ST446). SHEFM2K is large contractile-tailed jumbophage with a genome of 348 kb sharing homology with jumbophage from <em>E. coli</em> of the <em>Asteriusvirus</em> genus. SHEF-M2K host range testing indicated that it only makes clear plaques with EcM2K and a sepsis strain from our collection (G34590). Host-ranging assays indicated that it is able to suppress the growth of a range of <em>E. coli</em> strains in liquid culture assays: including EHEC O157:H7, K-12 (MC1000, MG1655) and <em>E.coli</em> B (BL21). TEM images of infection of EcM2K indicated association with flagella-like structures. An <em>E. coli</em> MC1000 mutant lacking the flagellin (<em>fliC</em>) gene was less sensitive to SHEFM2K infection, a phenotype restored by providing <em>fliC in trans</em>. These data illustrate M2K is a flagellotrophic phage that attaches to flagella as part of its infection cycle. We also present cross-sectional TEM images of the SHEFM2K infection cycle showing that it forms putative ‘assembly areas’ in the host cytoplasm cleared of ribosomes and other material with heads appearing within the periphery before tails appear and lysis occurs. We also present the proteome of mature SHEFM2K phage, highlighting proteins expressed and notable those no detected which might have a role in replication given their predicted function. Overall, we present a preliminary characterisation of a newly isolated jumbophage that interacts with the <em>E. coli</em> flagellum and uncover novel aspects of their biology by identifying an internal assembly area.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128082"},"PeriodicalIF":6.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet-driven diversity of antibiotic resistance genes in wild bats: implications for public health","authors":"Long Huang ,&nbsp;Wentao Dai ,&nbsp;Xiaoyu Sun ,&nbsp;Yingting Pu ,&nbsp;Jiang Feng ,&nbsp;Longru Jin ,&nbsp;Keping Sun","doi":"10.1016/j.micres.2025.128086","DOIUrl":"10.1016/j.micres.2025.128086","url":null,"abstract":"<div><div>Wild bats may serve as reservoirs for antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria, potentially contributing to antibiotic resistance and pathogen transmission. However, current assessments of bats’ antibiotic resistance potential are limited to culture-dependent bacterial snapshots. In this study, we present metagenomic evidence supporting a strong association between diet, gut microbiota, and the resistome, highlighting bats as significant vectors for ARG propagation. We characterized gut microbiota, ARGs, and mobile genetic elements (MGEs) in bats with five distinct diets: frugivory, insectivory, piscivory, carnivory, and sanguivory. Our analysis revealed high levels of ARGs in bat guts, with limited potential for horizontal transfer, encompassing 1106 ARGs conferring resistance to 26 antibiotics. Multidrug-resistant and polymyxin-resistant genes were particularly prevalent among identified ARG types. The abundance and diversity of ARGs/MGEs varied significantly among bats with different dietary habits, possibly due to diet-related differences in microbial composition. Additionally, genetic linkage between high-risk ARGs and multiple MGEs was observed on the genomes of various zoonotic pathogens, indicating a potential threat to human health from wild bats. Overall, our study provides a comprehensive analysis of the resistome in wild bats and underscores the role of dietary habits in wildlife-associated public health risks.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128086"},"PeriodicalIF":6.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of the soil microbiome in sustaining grassland ecosystem health on the Qinghai-Tibet Plateau","authors":"Mingxu Zhang ,&nbsp;Jinpeng Hu ,&nbsp;Yuewei Zhang ,&nbsp;Yanhua Cao ,&nbsp;Christopher Rensing ,&nbsp;Quanmin Dong ,&nbsp;Fujiang Hou ,&nbsp;Jinlin Zhang","doi":"10.1016/j.micres.2025.128078","DOIUrl":"10.1016/j.micres.2025.128078","url":null,"abstract":"<div><div>Soil microbes, as intermediaries in plant-soil interactions, are closely linked to plant health in grassland ecosystems. In recent years, varying degrees of degradation have been observed in the alpine grasslands of the Qinghai-Tibet Plateau (QTP). Addressing grassland degradation, particularly under the influence of climate change, poses a global challenge. Understanding the factors driving grassland degradation on the QTP and developing appropriate mitigation measures is essential for the future sustainability of this fragile ecosystem. In this review, we discuss the environmental and anthropogenic factors affecting grassland degradation and the corresponding impacts on soil microbe community structure. We summarize the current research on the microbiome of the QTP, in particular the effect of vegetation, climate change, grazing, and land use, respectively on the alpine grassland microbiome. The results of these studies indicate that microbially mediated soil bioprocesses are important drivers of grassland ecosystem functional recovery. Therefore, a thorough understanding of the spatial distribution characteristics of the soil microbiome in alpine grasslands is required, and this necessitates an integrated approach in which the interactions among climatic factors, vegetation characteristics, and human activities are evaluated. Additionally, we assess and summarise current technological developments and prospects for applying soil microbiome technologies in sustainable agriculture, including: (i) single-strain inoculation, and (ii) inoculation of synthetic microbial communities, (iii) microbial community transplantation. Grassland restoration projects should be carried out with the understanding that each restoration measure has a unique effect on the soil microbial activity. We propose that the sustainable development of alpine grassland ecosystems can be achieved by adopting advanced microbiome technologies and integrating microbe-based sustainable agricultural practices to maximise grassland biomass, increase soil carbon, and optimise soil nutrient cycling.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"293 ","pages":"Article 128078"},"PeriodicalIF":6.1,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}