Microbiological research最新文献

{"title":"Microbial strategies for soda saline-alkali soil remediation: The role of haloalkaliphilic bacteria","authors":"Bonaventure Chidi Ezenwanne ,&nbsp;Charles Obinwanne Okoye ,&nbsp;Huifang Jiang ,&nbsp;Lu Gao ,&nbsp;Xunfeng Chen ,&nbsp;Yanfang Wu ,&nbsp;Jianxiong Jiang","doi":"10.1016/j.micres.2025.128410","DOIUrl":"10.1016/j.micres.2025.128410","url":null,"abstract":"<div><div>Global agriculture is increasingly constrained by soil degradation, with salinization and alkalization reducing crop productivity, soil function, and long-term ecosystem stability. Among salt-affected soils, soda saline-alkali soils represent a particularly challenging subtype, characterized by excessive accumulation of soluble salts, elevated pH, and high sodium content, all of which exacerbate soil structural decline. Haloalkaliphilic bacteria, adapted to high salinity and alkalinity, offer a sustainable bioremediation strategy. This review presents a conceptual framework elucidating the mechanisms by which haloalkaliphilic bacteria mitigate soda saline-alkali stress through osmoprotectant synthesis, ion homeostasis regulation, pH neutralization, extracellular polymeric substance (EPS) formation, and extremozyme activity, thereby enhancing nutrient mobilization and organic-matter turnover. These microbial processes facilitate contaminant degradation and stimulate plant growth by improving nutrient availability and promoting phytohormone production. The resulting plant-microbe synergy translates microbial activity into enhanced soil function by reducing bulk salinity and pH, improving structure and water retention, and promoting overall soil fertility. This review further identifies critical challenges to translating mechanistic insights into field practice, including ecological variability, inoculant efficacy and resilience, regulatory frameworks, scalable inoculant manufacturing, a paucity of multi-season field trials, and socioeconomic constraints. Prospects include integrative multi-omics to link gene expression with ecosystem outcomes; systematic exploration of extremozymes; incorporation of nutrient-rich biomass for consortium support; AI-guided consortia design and predictive modeling for site-specific optimization, and long-term monitoring. These strategies enhance our understanding of tolerance to high salinity and alkalinity, paving the way for innovative microbial interventions to restore soda saline-alkali soils and support more resilient, sustainable agricultural systems.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"304 ","pages":"Article 128410"},"PeriodicalIF":6.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145668962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative multi-omics elucidates the impact of microalgae on growth, quality, phytohormones, and rhizosphere microbiome of Angelica sinensis","authors":"Tao Yang ,&nbsp;Yulong Zhan ,&nbsp;Jie Sha ,&nbsp;Jiang Zhao ,&nbsp;Chengniu Wang ,&nbsp;Tong Peng ,&nbsp;Lei Zhang","doi":"10.1016/j.micres.2025.128418","DOIUrl":"10.1016/j.micres.2025.128418","url":null,"abstract":"<div><div>Microalgae have recently been recognized as sustainable biofertilizers that improve soil fertility while enhancing crop performance. However, their roles in regulating medicinal plant growth and quality, as well as the underlying ecological mechanisms, remain poorly understood. In this study, we systematically assessed the effects of three representative microalgae—<em>Anabaena cylindrica</em> (AC), <em>Phormidium tenue</em> (PT), and <em>Chlorella vulgaris</em> (CV)—on the growth, quality, hormonal regulation, soil nutrient dynamics, and rhizosphere microbiome of <em>Angelica sinensis</em>. Field inoculation trials demonstrated that all three microalgae significantly promoted biomass accumulation and increased antioxidant capacity. AC and CV further enhanced the accumulation of ferulic acid and flavonoids, which are two key quality determinants. Microalgal inoculation significantly altered rhizosphere soil properties by increasing total organic carbon and alkali-hydrolyzable nitrogen, with AC uniquely elevating available phosphorus and iron. Metagenomic analysis revealed that AC and PT stimulated nitrification while suppressing denitrification, thereby reducing nitrogen loss and stabilizing the soil nitrogen pools. Distinct microbial taxa, including <em>Rhodanobacter</em>, <em>Streptomyces</em>, and <em>Pseudomonas</em>, were identified as the major contributors to carbon and nitrogen cycling. Hormone metabolomics showed that microalgal inoculation reprogrammed <em>A</em>. <em>sinensis</em> phytohormone profiles in a species-specific manner. Partial least squares path modeling suggested that AC and CV promote ferulic acid biosynthesis through distinct mechanisms, with AC associated with reduced investment in C-mineralization processes and CV associated with lower salicylic acid levels, whereas PT enhances biomass accumulation mainly by stimulating N-cycle processes. Collectively, this study provides integrated evidence linking microalgae-mediated nutrient cycling, rhizosphere microbiome shifts and hormonal regulation to enhanced quality formation in <em>A</em>. <em>sinensis</em>.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"304 ","pages":"Article 128418"},"PeriodicalIF":6.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the dynamics of Pseudomonas syringae tailocin targeting allows for predictive protective microbial inoculation of Actinidia chinensis","authors":"Banyon H. Carnell ,&nbsp;Jay Jayaraman ,&nbsp;Jose Benjamin P. Dar Juan ,&nbsp;Matthew D. Templeton ,&nbsp;Iain D. Hay","doi":"10.1016/j.micres.2025.128401","DOIUrl":"10.1016/j.micres.2025.128401","url":null,"abstract":"<div><div>The <em>Pseudomonas syringae</em> complex is an important group within the Gammaproteobacteria and comprises several pathovars of agricultural significance. Genome mining of the <em>P. syringae</em> species complex has uncovered high-molecular-weight phage tail complexes termed tailocins. Tailocins exert specific bactericidal action against both closely and more distantly related bacteria and significantly shape the ecology of the microbiome. Tailocin targeting specificity is currently understood to be dependent on tail-fibers (TFs) binding to specific molecular epitopes, including lipopolysaccharide (LPS) as a bacterial cell surface receptor for tailocin TF-targeting domains. Recent work in <em>P. syringae</em> has strongly correlated variation at the common polysaccharide antigen of LPS with tailocin sensitivity. Here we provide biochemical evidence for LPS as the major receptor for <em>P. syringae</em> tailocins; examine the mechanisms and genetic basis of tailocin TF targeting; and predict strains that can provide protective colonization of plants. We then use the understanding of these mechanisms that determine the tailocin targeting spectrum and genetic knockouts and complementation to modify the bacterial canker pathogen of kiwifruit plants to predict LPS-mediated tailocin targeting by naturally occurring host microbiota, and then demonstrate the efficacy of these applied microbiome-derived tailocin-carrying commensal strains as biocontrol agents.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"304 ","pages":"Article 128401"},"PeriodicalIF":6.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145682410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of novel Vibrio phages as potential biocontrol agents against Vibrio alginolyticus and insights into its phage-resistant mutant","authors":"Roshan Nepal ,&nbsp;Tony Charles ,&nbsp;George Bouras ,&nbsp;Richard S. Taylor ,&nbsp;James W. Wynne","doi":"10.1016/j.micres.2025.128412","DOIUrl":"10.1016/j.micres.2025.128412","url":null,"abstract":"<div><div>Pathogenic bacteria are an ongoing threat to intensive agriculture, including aquaculture. With the emergence of antimicrobial resistance (AMR), novel non-traditional antimicrobials are urgently needed to minimize the dependence on antibiotics. Bacteriophage (phage) therapy, which uses naturally occurring viruses to kill specific bacterium, is gaining interest and offers huge potential for targeted pathogen control in aquaculture. However, many challenges regarding stability and emergence of phage-resistance must be overcome. Here, we isolated and characterized eight Vibrio phages against an emerging aquaculture pathogen <em>Vibrio alginolyticus</em> and studied their bactericidal and antibiofilm potency. We then used next-generation sequencing to understand how the <em>Vibrio</em> species may gain phage resistance. Our results indicated that most of the isolated Vibrio phages (seven out of eight) shared &lt; 40 % genomic similarity with phages isolated elsewhere, possibly suggesting novel strains. The phages were stable in different temperatures (4–40 °C), pHs (3−10) and salinities (0–50 ppt) up to 6 h without significant loss in viability. Although individual phages had variable bactericidal efficiency and bacteria rapidly developed phage-resistance, phage cocktail formulations were highly efficient and significantly suppressed bacterial growth up to 15 h, inhibited biofilm formation (p &lt; 0.05) and eradicated established biofilms (p &lt; 0.05). Sequencing confirmed absence of lysogeny modules, known toxins and AMR genes in seven of the phages. Further, tRNAs and a putative anti-CRISPR (Acr) protein was found in two of the most efficient phages. Though bacteria rapidly developed phage-resistance, we observed increased antibiotic sensitivity as a trade-off which possibly resulted from defective efflux pump. Our findings support potential applications of Vibrio phages in aquaculture systems for minimizing the burden of Vibriosis. However, further research is required to elucidate the role of efflux pump system in phage-resistance and antimicrobial resistance.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"304 ","pages":"Article 128412"},"PeriodicalIF":6.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial acetyl-CoA synthesis as an emerging metabolic and regulatory hub in plant-microbe interactions","authors":"Yanan Zhou ,&nbsp;Xue-Xian Zhang ,&nbsp;Dandan Wang ,&nbsp;Mengguang Zhao ,&nbsp;Li Sun ,&nbsp;Weiwei Huang ,&nbsp;Zhihong Xie","doi":"10.1016/j.micres.2025.128413","DOIUrl":"10.1016/j.micres.2025.128413","url":null,"abstract":"<div><div>Acetyl-CoA synthetase (ACS) is a well-characterized enzyme that catalyzes the ATP-dependent ligation of acetate and coenzyme A to produce acetyl-CoA, a central metabolite coordinating energy metabolism, carbon flux distribution, and post-translational protein modification. Recently, ACS has emerged as a metabolic nexus with broad implications for plant–microbe interactions in agriculture. Beyond its canonical role in primary metabolism, ACS governs diverse physiological processes in beneficial plant-associated microorganisms, including rhizosphere colonization, stress adaptation, secondary metabolite biosynthesis, and morphological development—all of which enhance plant growth and resilience. In contrast, in phytopathogens, ACS is closely related to the expression of virulence factors. Thus, ACS exerts a dual influence, shaping both mutualistic and antagonistic microbial lifestyles <em>in planta</em>. This review synthesizes recent advances in the structural and catalytic diversity of ACS, delineates its ecological and functional roles in agriculturally relevant microorganisms, and explores the environmental and host-derived signals that regulates its expression and activity. Particular attention is given to the interplay between ACS-mediated carbon metabolism and protein acetylation, which together modulate microbial physiology and plant-associated behaviors. ACS is thereby positioned as a strategic metabolic hub, providing a framework for future research at the interface of microbial metabolism, environmental adaptation, and plant health.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"304 ","pages":"Article 128413"},"PeriodicalIF":6.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145714781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface-layer proteins of Enterococcus faecium WEFA23 inhibit Listeria monocytogenes-induced inflammation via TLR2-mediated NF-κB and MAPK signalling in RAW 264.7 cells","authors":"Yao He ,&nbsp;Xingyi Long ,&nbsp;Bing Dong ,&nbsp;Yina Huang ,&nbsp;Xueying Tao ,&nbsp;Hua Wei","doi":"10.1016/j.micres.2025.128373","DOIUrl":"10.1016/j.micres.2025.128373","url":null,"abstract":"<div><div><em>Listeria monocytogenes</em> infection, which has a higher mortality rate than other foodborne pathogens, is a major concern in global food safety. Among various interventions, lactic acid bacteria and their metabolites have gained significant attention. Our previous study showed that the surface layer proteins (SLPs) of <em>Enterococcus faecium</em> WEFA23, isolated from the gastrointestinal tract of newborn infants, exhibited strong inhibitory activity against <em>L. monocytogenes</em> CMCC57007; however, the underlying mechanism remained unclear. In this study, the immunomodulatory effects of <em>E. faecium</em> WEFA23 SLPs against <em>L. monocytogenes</em> infection were investigated in RAW 264.7 macrophage cells, focusing on phagocytic and bactericidal activity, as well as cytokine production. Furthermore, LC-MS/MS analysis indicated that ornithine carbamoyltransferase (OTC) could be a functional component of the SLPs. Consequently, <em>otc</em> gene knockout and heterologous expression strains were constructed. The results showed that deletion of the <em>otc</em> gene eliminated the inhibitory activity, while recombinant OTC maintained a satisfactory inhibitory effect. Mechanistically, both SLPs and purified OTC suppressed the activation of the NF-κB/MAPK signalling pathways, which were likely mediated through TLR2. Overall, our findings provide a scientific basis for the application of SLPs and <em>E. faecium</em> in food systems to prevent pathogenic infections.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128373"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145323195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere microbiota diversity and salt stress–alleviating functional genes in coastal wild salt-tolerant plants","authors":"Xixi Li ,&nbsp;Xiaojing Gao ,&nbsp;Shuyao Yu ,&nbsp;Fengfeng Du ,&nbsp;Jixiang Liu ,&nbsp;Xuhui Kan ,&nbsp;Xiaojing Liu ,&nbsp;Dongrui Yao","doi":"10.1016/j.micres.2025.128397","DOIUrl":"10.1016/j.micres.2025.128397","url":null,"abstract":"<div><div>Saline–alkali land significantly threatens global food security and ecological safety, and root-associated microorganisms help plants survive salt–alkali stress. However, the ecological functions and factors that influence the rhizosphere microbiomes of salt-tolerant plants remain poorly understood. In this study, we used high-throughput sequencing and metagenomics to reveal the microbial communities and functional traits of bulk and rhizosphere soil from salt-tolerant species (<em>Suaeda glauca</em>, <em>Phragmites australis</em>, and <em>Spartina alterniflora</em>) growing in saline soil. Bacterial and fungal taxa were significantly enriched in the rhizosphere soil compared to the non-rhizosphere soil. Metagenomic analyses revealed that metabolic pathways, including glycolysis and ABC transporters, were highly enriched in the rhizosphere. Functional profiling indicated that salt stress-related pathways were more abundant in the core genera <em>Pseudomonas</em> and <em>Woeseia</em>. The abundance of functional genes related to plant growth–promoting traits, including phosphate solubilization and salt adaptation pathways, was higher in the rhizosphere soil than in the non-rhizosphere soil, which was mainly driven by soil salinity, total nitrogen content, and total carbon content. Additionally, <em>P. aeruginosa</em> obtained from the rhizosphere of <em>S. alterniflora</em> exhibited high phosphorus solubilization efficiency (908.38 μg/mL), nitrogen fixation activity (2.84 μg/mL) and salt tolerance (≦ 5 % NaCl). These findings demonstrate that salt-tolerant plants shape microbial activities by controlling the rhizosphere microenvironment, mitigating salt stress, providing a scientific and practical foundation for the development of targeted microbial inoculants for saline–alkali land reclamation.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128397"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endofungal bacteria: Emerging paradigms and future directions","authors":"Adrian Wallner ,&nbsp;Utkarsh Talukdar ,&nbsp;Aurélie Deveau ,&nbsp;Julia Buchner ,&nbsp;Deepak Kumar ,&nbsp;Pooja Shukla ,&nbsp;Stéphane Compant ,&nbsp;Satish K. Verma","doi":"10.1016/j.micres.2025.128361","DOIUrl":"10.1016/j.micres.2025.128361","url":null,"abstract":"<div><div>The colonization of fungal hyphae and spores by bacteria represents a widespread phenomenon with significant ecological and biotechnological implications across all surveyed fungal phyla. First thought to be restricted to <em>Mollicutes</em> and <em>Burkholderiaceae</em>-Related Endobacteria, these endofungal associations exhibit remarkable diversity, from simple uniform populations to complex communities, contradicting earlier assumptions of uniform populations. Acquisition dynamics demonstrate both ancient co-evolutionary relationships and recent horizontal transfer events, with environmental factors driving strain-level variation in symbiont presence even within the same fungal species. Fungi can harbor either uniform or diverse bacterial communities, sometimes within specialized structures, and exhibit varying degrees of dependence on their symbionts. These interactions can be mutualistic, commensal, or parasitic, influencing fungal physiology, metabolism, and ecological function. Yet the underlying mechanisms have been thoroughly characterized in only a few model systems in which endofungal bacteria have been shown to affect nutrient acquisition, stress tolerance, secondary metabolite production, and even fungal pathogenicity. In return, fungi offer a protective niche and promote dispersion. These concepts collectively illustrate the evolutionary flexibility and ecological importance of fungi-bacteria partnerships across terrestrial ecosystems.</div><div>This review synthesizes emerging paradigms in endofungal bacteria research, integrating recent discoveries that challenge traditional assumptions about these symbioses. We examine host specificity patterns, acquisition mechanisms, and functional impacts while identifying critical knowledge gaps requiring investigation. A deeper understanding of these associations is essential to establish standardized frameworks for their applications in agriculture, medicine, and environmental sustainability.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128361"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145289928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eight-year effect of biochar amendment on soil properties, extracellular enzyme activity, N-cycling genes and microbiome structure in two Danish fallow soils","authors":"Paul Iturbe-Espinoza ,&nbsp;Lars Elsgaard ,&nbsp;Rumakanta Sapkota ,&nbsp;Lea Ellegaard-Jensen ,&nbsp;Anne Winding","doi":"10.1016/j.micres.2025.128398","DOIUrl":"10.1016/j.micres.2025.128398","url":null,"abstract":"<div><div>Biochar improves agricultural soil properties and short-term microbial diversity. However, biochar’s long-term effects on microbiomes and soil health remain poorly understood. This study assessed the effects of 8-year field-aged biochar on microbiomes from two contrasting soils: a sandy clay soil and a coarse sandy soil, under temperate climate conditions. We hypothesize that even after 8 years, biochar amendment persistently alters soil physicochemical properties, stimulates extracellular enzyme activity, increases the abundance of N-cycling genes, and shifts the prokaryotic and fungal community structures. In June 2015, the topsoil in field lysimeters was amended with 2 % w/w straw biochar, and by August 2023, this biochar amendment resulted in a significant increased activity of five key extracellular enzymes (α-glucosidase, β-galactosidase, cellobiosidase, phosphomonoesterase, and arylsulfatase) involved in C, P, and S cycling in both soils. In the coarse sandy soil, biochar boosted the abundance of prokaryotes (16S rRNA gene), key nitrification genes (AOA-<em>amoA</em> and AOB-<em>amoA</em>), and the denitrification gene <em>nosZ</em> Clade I. In both soils, biochar caused an increase in the abundance of the nitrite reductase (<em>nirS</em>) gene, indicating a sustained impact on the N cycle, and an enrichment of an ammonia-oxidizing archaeon of the family <em>Nitrosophaeraceae.</em> Finally, a persistent shift in prokaryotic community structure was observed in both soils. The study clearly demonstrates that the effects of biochar persist after eight years, providing insights into the long-term impact of biochar on soil health.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128398"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic drivers of PAHs transformation in the spatial and temporal continuum of the rhizosphere: An analysis of plant-microbe synergistic mechanism","authors":"Jieting Wu ,&nbsp;Haoran Yin ,&nbsp;Yuxin Li ,&nbsp;Lei Zhao ,&nbsp;Haijuan Guo ,&nbsp;Chengbin Xu ,&nbsp;Jing Shang ,&nbsp;Xiaofan Fu ,&nbsp;Fang Ma ,&nbsp;Ruoning Song","doi":"10.1016/j.micres.2025.128380","DOIUrl":"10.1016/j.micres.2025.128380","url":null,"abstract":"<div><div>The migration and transformation of polycyclic aromatic hydrocarbons (PAHs) in soil systems are inherently constrained by their low solubility, strong sorption affinity to soil particles, and limited bioavailability for biological uptake and degradation. As a critical ecological interface mediating plant-microbe interactions, the rhizosphere plays a pivotal role in facilitating PAHs mobilization and transformation processes. This review systematically examines the spatiotemporal dynamics of PAHs migration and transformation within rhizosphere systems under plant-microbe collaborative regulation, characterized by sequential temporal phases (initial desorption-solubilization, intermediate absorption-accumulation, and terminal degradation-transformation) and spatial stratification (rhizosphere soil-liquid interface, root surface microenvironment, and internal root tissues). We particularly emphasize the synergistic plant-microbe interactions that drive PAHs desorption, solubilization, phytoaccumulation, and biodegradation. Furthermore, we elucidate four potential mechanistic pathways: AHL analogs in root exudates activate bacterial quorum sensing systems to stimulate surfactant production and PAHs-degrading enzyme synthesis; Microbial-derived IAA enhances H⁺ -ATPase activity in plants, facilitating PAHs/H⁺ co-transport mechanisms; Coordinated AHL-IAA signaling promotes Ca²⁺ uptake and upregulates root nodule symbiosis-related gene expression; ROS in root exudates activate bacterial c-di-GMP signaling pathways to enhance microbial colonization and PAHs-degrading enzyme production. We also analyze the practical limitations affecting rhizoremediation efficacy, including climatic conditions, soil heterogeneity, and variations in pollutant types, and propose corresponding future research directions toward the end of the article. This comprehensive analysis establishes a theoretical framework for understanding the mechanistic basis of plant-microbe synergism in rhizospheric PAHs remediation, offering a foundation for advancing rhizosphere engineering and phytoremediation strategies.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"303 ","pages":"Article 128380"},"PeriodicalIF":6.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}