Keystone root bacteria in Ambrosia artemisiifolia promote invasive growth by increasing the colonization rate of Funneliformis mosseae

Higher arbuscular mycorrhizal fungi (AMF) colonization rates in the roots of invasive plants than in those of native plants are associated with invasion success. Keystone plant-root bacteria (or root-associated bacteria) can influence plant growth by interacting with other members of the microbial community (eg.AMF). We aimed to investigate the effects of keystone taxa on AMF colonization and their interactions on invasive plant growth. Here, the common key root-associated species from the roots of Ambrosia artemisiifolia among four geographical populations in China were identified, and the strains were subsequently isolated. Plate and pot experiments were conducted to examine the impact of keystone species on the colonization of Funneliformis mosseae and elucidate the mechanisms that enhance plant growth. Sphingomonas was identified as a common keystone root-associated genus of A. artemisiifolia. Sphingomonas sanxanigenens was found to facilitate AMF colonization in the roots of A. artemisiifolia by promoting flavonoid biosynthesis. A synergistic effect on the growth of A. artemisiifolia was observed when the plant was co-inoculated with S. sanxanigenens and F. mosseae. This study provides new insights into the mechanisms whereby root-associated microbes facilitate AMF colonization in invasive plants. These findings confirm the pivotal role of keystone microbes in weed invasion and enhance our understanding that microbial synergistic interactions promote weed invasiveness.