Organoids in host pathology: Unveiling microbial-induced therapeutic responses

Abstract

Organoids are three-dimensional (3D) cultures derived from stem cells that replicate the complex structure and function of human tissues. They provide a physiologically relevant platform for studying microbial-host interactions, offering insights into pathogen-induced host lesions and therapeutic responses. By mimicking the microenvironment of organs such as the intestine, lung, and brain, organoids were applied in microbial pathogenesis, immune responses, and drug efficacy. Despite challenges in simulating immune dynamics and chronic infections, advancements in vascularization, immune component integration, and 3D bioprinting hold promise for breakthroughs in infectious disease research and precision medicine. This review highlights the transformative potential of organoids in understanding the mechanisms of pathogen infection and developing targeted therapies.