Microbiology and Molecular Biology Reviews最新文献

Vitamin biosynthesis in the gut: interplay between mammalian host and its resident microbiota. 肠道中维生素的生物合成：哺乳动物宿主及其常驻微生物群之间的相互作用。

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-25 Epub Date: 2025-04-02 DOI: 10.1128/mmbr.00184-23

Chiara Tarracchini, Cathy Lordan, Christian Milani, Luiza P D Moreira, Qusai M Alabedallat, Alejandra de Moreno de LeBlanc, Francesca Turroni, Gabriele Andrea Lugli, Leonardo Mancabelli, Giulia Longhi, Lorraine Brennan, Jennifer Mahony, Jean Guy LeBlanc, Kanishka N Nilaweera, Paul D Cotter, Douwe van Sinderen, Marco Ventura

{"title":"Vitamin biosynthesis in the gut: interplay between mammalian host and its resident microbiota.","authors":"Chiara Tarracchini, Cathy Lordan, Christian Milani, Luiza P D Moreira, Qusai M Alabedallat, Alejandra de Moreno de LeBlanc, Francesca Turroni, Gabriele Andrea Lugli, Leonardo Mancabelli, Giulia Longhi, Lorraine Brennan, Jennifer Mahony, Jean Guy LeBlanc, Kanishka N Nilaweera, Paul D Cotter, Douwe van Sinderen, Marco Ventura","doi":"10.1128/mmbr.00184-23","DOIUrl":"10.1128/mmbr.00184-23","url":null,"abstract":"SUMMARYIn recent years, exhaustive efforts have been made to dissect the composition of gut-associated microbial communities and associated interactions with their human host, which are thought to play a crucial role in host development, physiology, and metabolic functions. Although such studies were initially focused on the description of the compositional shifts in the microbiota that occur between different health conditions, more recently, they have provided key insights into the functional and metabolic contributions of the gut microbiota to overall host physiology. In this context, an important metabolic activity of the human gut microbiota is believed to be represented by the synthesis of various vitamins that may elicit considerable benefits to human health. A growing body of scientific literature is now available relating to (predicted) bacterial vitamin biosynthetic abilities, with ever-growing information concerning the prevalence of these biosynthetic abilities among members of the human microbiota. This review is aimed at disentangling if and how cooperative trophic interactions of human microbiota members contribute to vitamin production, and if such, gut microbiota-mediated vitamin production varies according to different life stages. Moreover, it offers a brief exploration of how different diets may influence vitamin production by shaping the overall composition and metabolic activity of the human gut microbiota while also providing preliminary insights into potential correlations between human microbiota-associated vitamin production and the occurrence of human diseases and/or metabolic disorders.","PeriodicalId":18520,"journal":{"name":"Microbiology and Molecular Biology Reviews","volume":" ","pages":"e0018423"},"PeriodicalIF":8.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A roadmap to understanding and anticipating microbial gene transfer in soil communities. 了解和预测土壤群落中微生物基因转移的路线图。

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-25 Epub Date: 2025-04-08 DOI: 10.1128/mmbr.00225-24

David L Gillett, Malyn Selinidis, Travis Seamons, Dalton George, Alexandria N Igwe, Ilenne Del Valle, Robert G Egbert, Kirsten S Hofmockel, Alicia L Johnson, Kirstin R W Matthews, Caroline A Masiello, Lauren B Stadler, James Chappell, Jonathan J Silberg

{"title":"A roadmap to understanding and anticipating microbial gene transfer in soil communities.","authors":"David L Gillett, Malyn Selinidis, Travis Seamons, Dalton George, Alexandria N Igwe, Ilenne Del Valle, Robert G Egbert, Kirsten S Hofmockel, Alicia L Johnson, Kirstin R W Matthews, Caroline A Masiello, Lauren B Stadler, James Chappell, Jonathan J Silberg","doi":"10.1128/mmbr.00225-24","DOIUrl":"10.1128/mmbr.00225-24","url":null,"abstract":"SUMMARYEngineered microbes are being programmed using synthetic DNA for applications in soil to overcome global challenges related to climate change, energy, food security, and pollution. However, we cannot yet predict gene transfer processes in soil to assess the frequency of unintentional transfer of engineered DNA to environmental microbes when applying synthetic biology technologies at scale. This challenge exists because of the complex and heterogeneous characteristics of soils, which contribute to the fitness and transport of cells and the exchange of genetic material within communities. Here, we describe knowledge gaps about gene transfer across soil microbiomes. We propose strategies to improve our understanding of gene transfer across soil communities, highlight the need to benchmark the performance of biocontainment measures in situ, and discuss responsibly engaging community stakeholders. We highlight opportunities to address knowledge gaps, such as creating a set of soil standards for studying gene transfer across diverse soil types and measuring gene transfer host range across microbiomes using emerging technologies. By comparing gene transfer rates, host range, and persistence of engineered microbes across different soils, we posit that community-scale, environment-specific models can be built that anticipate biotechnology risks. Such studies will enable the design of safer biotechnologies that allow us to realize the benefits of synthetic biology and mitigate risks associated with the release of such technologies.","PeriodicalId":18520,"journal":{"name":"Microbiology and Molecular Biology Reviews","volume":" ","pages":"e0022524"},"PeriodicalIF":8.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host specificity of gut microbiota associated with social bees: patterns and processes. 与群居蜜蜂相关的肠道微生物群宿主特异性：模式和过程。

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-25 Epub Date: 2025-03-20 DOI: 10.1128/mmbr.00080-23

Florent Mazel, Aiswarya Prasad, Philipp Engel

{"title":"Host specificity of gut microbiota associated with social bees: patterns and processes.","authors":"Florent Mazel, Aiswarya Prasad, Philipp Engel","doi":"10.1128/mmbr.00080-23","DOIUrl":"10.1128/mmbr.00080-23","url":null,"abstract":"SUMMARYGut microbes provide benefits to some animals, but their distribution and effects across diverse hosts are still poorly described. There is accumulating evidence for host specificity (i.e., a pattern where different microbes tend to associate with distinct host lineages), but the causes and consequences of this pattern are unclear. Combining experimental tests in the laboratory with broad surveys in the wild is a promising approach to gaining a comprehensive and mechanistic understanding of host specificity prevalence, origin, and importance. Social bees represent an ideal testbed for this endeavor because they are phylogenetically and functionally diverse, with host-specific, stable, and tractable gut microbiota. Furthermore, the western honeybee (Apis mellifera) is an emerging experimental model system for studying microbiota-host interactions. In this review, we summarize data on the prevalence and strength of host specificity of the social bee gut microbiota (bumblebees, stingless bees, and honeybees), as well as the potential and proven ecological and molecular mechanisms that maintain host specificity. Overall, we found that host specificity in bees is relatively strong and likely results from several processes, including host filtering mediated by the immune system and priority effects. However, more research is needed across multiple social bee species to confirm these findings. To help future research, we summarize emerging hypotheses in the field and propose several experimental and comparative tests. Finally, we conclude this review by highlighting the need to understand how host specificity can influence host health.","PeriodicalId":18520,"journal":{"name":"Microbiology and Molecular Biology Reviews","volume":" ","pages":"e0008023"},"PeriodicalIF":8.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobiome: an underexplored kingdom in cancer. 霉菌生物群：一个未被充分探索的癌症王国。

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-25 Epub Date: 2025-03-14 DOI: 10.1128/mmbr.00261-24

Yan-Yan Sun, Ning-Ning Liu

引用次数: 0

O-antigen polysaccharides in Klebsiella pneumoniae: structures and molecular basis for antigenic diversity. 肺炎克雷伯菌的o抗原多糖：抗原多样性的结构和分子基础。

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-25 Epub Date: 2025-03-21 DOI: 10.1128/mmbr.00090-23

Chris Whitfield, Steven D Kelly, Tom D Stanton, Kelly L Wyres, Bradley R Clarke, Taylor J B Forrester, Agnieszka Kowalczyk

{"title":"O-antigen polysaccharides in Klebsiella pneumoniae: structures and molecular basis for antigenic diversity.","authors":"Chris Whitfield, Steven D Kelly, Tom D Stanton, Kelly L Wyres, Bradley R Clarke, Taylor J B Forrester, Agnieszka Kowalczyk","doi":"10.1128/mmbr.00090-23","DOIUrl":"10.1128/mmbr.00090-23","url":null,"abstract":"SUMMARYKlebsiella pneumoniae is a gram-negative species, whose isolates are found in the environment and as commensals in the human gastrointestinal tract. This bacterium is among the leading causes of a range of nosocomial and community-acquired infections, particularly in immunocompromised individuals, where it can give rise to pneumonia, urinary tract infections, septicemia, and liver abscesses. Treatment of K. pneumoniae infections is compromised by the emergence of isolates producing carbapenemase and extended-spectrum β-lactamase enzymes, making it a high priority for new therapeutic approaches including vaccination and immunoprophylaxis. One potential target for these strategies is the O-antigen polysaccharide component of lipopolysaccharides, which are important virulence determinants for K. pneumoniae. Consideration of immunotherapeutic opportunities requires a comprehensive and fundamental understanding of O-polysaccharide structures, distribution of particular O serotypes in clinical isolates, and the potential for antigenic diversification. The number of recognized K. pneumoniae O-polysaccharide antigens has varied over time, complicated by the observation that some examples share similar structural (and potentially antigenically cross-reactive) elements, and by the existence of genetic loci for which corresponding O-polysaccharide structures have yet to be determined. Here, we provide a comprehensive integration of the current carbohydrate structures and genetic information, together with a proposal for an updated classification system for K. pneumoniae O-antigens, that is being implemented in Kaptive for molecular serotyping. The accumulated insight into O-polysaccharide assembly pathways is used to describe the molecular basis for O-antigen diversity in K. pneumoniae.","PeriodicalId":18520,"journal":{"name":"Microbiology and Molecular Biology Reviews","volume":" ","pages":"e0009023"},"PeriodicalIF":8.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral factors underlying the pandemic potential of influenza viruses. 流感病毒具有大流行潜力的病毒因素。

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-25 Epub Date: 2025-05-09 DOI: 10.1128/mmbr.00066-24

Gabriele Neumann, Amie J Eisfeld, Yoshihiro Kawaoka

引用次数: 0

Coinfection by multiple Trypanosoma cruzi clones: a new perspective on host-parasite relationship with consequences for pathogenesis and management of Chagas disease. 多重克氏锥虫克隆的共同感染：宿主-寄生虫关系与恰加斯病发病机制和管理后果的新视角

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-25 Epub Date: 2025-03-21 DOI: 10.1128/mmbr.00242-24

Yves Carlier, Eric Dumonteil, Claudia Herrera, Etienne Waleckx, Michel Tibayrenc, Pierre Buekens, Carine Truyens, Eric Muraille

{"title":"Coinfection by multiple Trypanosoma cruzi clones: a new perspective on host-parasite relationship with consequences for pathogenesis and management of Chagas disease.","authors":"Yves Carlier, Eric Dumonteil, Claudia Herrera, Etienne Waleckx, Michel Tibayrenc, Pierre Buekens, Carine Truyens, Eric Muraille","doi":"10.1128/mmbr.00242-24","DOIUrl":"10.1128/mmbr.00242-24","url":null,"abstract":"SUMMARYChagas disease (CD) is caused by the protozoan parasite Trypanosoma cruzi (Tc), infecting 6-7 million people. It is transmitted by insect vectors, orally, through infected tissues, or congenitally. Tc infection can progress toward chronic cardiac and/or digestive severe and fatal CD in 20%-40% of patients. Tc exhibits an important genetic and phenotypic intraspecies diversity and a preponderant clonal population structure. The impact of multiclonal coinfections has been little studied in CD patients. Relationships between the currently used discrete typing unit (DTU)-based classification of Tc lineages and the occurrence of the different clinical forms of CD, its congenital transmission, as well as the efficacy of trypanocidal molecules (benznidazole and nifurtimox) could not be established. In this review, we revisit the different aspects of Tc diversity and analyze the impact of infections with multiple clones and their variants on the dynamic and pathogenesis of CD and its maternal-fetal transmission. We propose to call \"cruziome\" all the Tc clones and their variants infecting a given host and provide strong evidence that (i) multiclonal Tc infections are likely the rule rather than the exception; (ii) each \"cruziome\" is associated with a unique combination of virulence factors, tissular tropisms, and host immune responses; (iii) accordingly, some particularly harmful \"cruziomes\" likely trigger the occurrence and progression of CD and might also favor the congenital transmission of parasites. We propose that our concept of \"cruziome\" should be taken into consideration because of its practical consequences in epidemiological studies, laboratory diagnosis, clinical management, and treatment of CD.","PeriodicalId":18520,"journal":{"name":"Microbiology and Molecular Biology Reviews","volume":" ","pages":"e0024224"},"PeriodicalIF":8.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty acid synthesis and utilization in gram-positive bacteria: insights from Bacillus subtilis. 革兰氏阳性菌脂肪酸的合成和利用：来自枯草芽孢杆菌的见解。

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-25 Epub Date: 2025-05-28 DOI: 10.1128/mmbr.00069-23

María Cecilia Mansilla, Diego de Mendoza

{"title":"Fatty acid synthesis and utilization in gram-positive bacteria: insights from Bacillus subtilis.","authors":"María Cecilia Mansilla, Diego de Mendoza","doi":"10.1128/mmbr.00069-23","DOIUrl":"10.1128/mmbr.00069-23","url":null,"abstract":"SUMMARYThe bacterial cytoplasmic membrane, consisting of roughly equal proportions of proteins and lipids, plays a crucial role in cellular growth, metabolism, and maintaining the cytoplasmic boundary. It is a dynamic, fluid matrix that separates intracellular compartments, where lipids and proteins coexist in a highly organized yet flexible arrangement. Membrane fluidity, defined as the inverse of viscosity, determines how rapidly molecules diffuse within the membrane at a given temperature. This property is vital for protein mobility and biomolecular interactions. Structurally, the membrane primarily comprises a lamellar lipid bilayer, with glycerophospholipids and fatty acids forming its core framework. In Bacillus subtilis, a key model organism for studying gram-positive bacterial physiology, major membrane lipids include phospholipids, glycolipids, and lipoteichoic acids, the latter anchored to diacylglycerol glycolipids. This review examines the synthesis and regulation of membrane lipids in B. subtilis, with a focus on fatty acid biosynthesis, its diversification, and post-synthetic modifications such as desaturation. It also explores the production of phosphatidic acid and the integration of fatty acid and phospholipid biosynthesis. We review the well-characterized pathway of cold-induced membrane lipid modification in B. subtilis, arguably the best-studied model system for temperature sensing. This pathway is tightly linked to transcriptional responses triggered by changes in bilayer viscosity, detected by a membrane-associated thermosensor. Finally, this review highlights the importance of fatty acid biosynthesis in B. subtilis differentiation and its contributions to the production of biotin and lipoic acid, two universal cofactors essential for fatty acid synthesis and intermediary metabolism.","PeriodicalId":18520,"journal":{"name":"Microbiology and Molecular Biology Reviews","volume":" ","pages":"e0006923"},"PeriodicalIF":8.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implications of morphological variation in influenza viruses. 流感病毒形态变异的意义。

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-20 DOI: 10.1128/mmbr.00015-25

Chidiebere F Uchechukwu, Nicole C Robb

引用次数: 0

Molecular mechanisms of SARS-CoV-2 entry: implications for biomedical strategies. SARS-CoV-2进入的分子机制：对生物医学策略的影响

IF 8 1区生物学

Microbiology and Molecular Biology Reviews Pub Date : 2025-06-12 DOI: 10.1128/mmbr.00260-24

Irene Santamaria-Castro, Rocio Leiva-Rebollo, Sonia Marín-Wong, Jose M Jimenez-Guardeño, Ana Maria Ortega-Prieto

{"title":"Molecular mechanisms of SARS-CoV-2 entry: implications for biomedical strategies.","authors":"Irene Santamaria-Castro, Rocio Leiva-Rebollo, Sonia Marín-Wong, Jose M Jimenez-Guardeño, Ana Maria Ortega-Prieto","doi":"10.1128/mmbr.00260-24","DOIUrl":"https://doi.org/10.1128/mmbr.00260-24","url":null,"abstract":"SUMMARYThe mechanisms by which viruses enter host cells are crucial for their ability to infect and cause disease, serving as major targets for both host immune responses and therapeutic strategies. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry process is primarily driven by the binding of the viral spike (S) protein to the angiotensin-converting enzyme 2 (ACE2) receptor, in conjunction with the activity of endosomal cathepsin L and the serine protease transmembrane protease serine 2 (TMPRSS2). Nevertheless, recent scientific advances have expanded our understanding of SARS-CoV-2 entry mechanisms, uncovering alternative receptors and novel cofactors that may enhance viral tropism and adaptability. Given the critical role of the SARS-CoV-2 S protein in mediating host cell entry, it has become a primary target for prevention and therapeutic strategies. However, the continuous spread of SARS-CoV-2 has led to the emergence of S protein variants that may potentially confer a fitness advantage or modify key aspects of SARS-CoV-2 biology, such as transmissibility, infectivity, antigenicity, and/or pathogenicity, posing significant challenges to the efficacy of current interventions. In this review, we provide an updated and comprehensive overview of the latest advances in SARS-CoV-2 entry pathways and molecular mechanisms, exploring their implications for antiviral drug discovery, vaccine design, and the development of other biomedical strategies while addressing the challenges posed by the ongoing evolution of the virus.","PeriodicalId":18520,"journal":{"name":"Microbiology and Molecular Biology Reviews","volume":" ","pages":"e0026024"},"PeriodicalIF":8.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0