Microscopy最新文献_第6页

Enhancement of image contrast for carbon nanotube and polymer composite film in scanning electron microscope 扫描电子显微镜增强碳纳米管和聚合物复合膜的图像对比度

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-11-01 DOI: 10.1093/jmicro/dfaa006

Yoichiro Hashimoto;Hiroyuki Ito;Masahiro Sasajima

引用次数: 2

For microscopy special issue on ‘AI and Imaging’ 显微镜“AI与成像”特刊

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-11-01 DOI: 10.1093/jmicro/dfaa009

Yasushi Okada;Yasukazu Murakami

引用次数: 0

Structured Illumination Approaches for Super-Resolution in Plant Cells 植物细胞超分辨率的结构光照方法

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI: 10.1093/jmicro/dfy043

Sidney L Shaw;David Thoms;James Powers

{"title":"Structured Illumination Approaches for Super-Resolution in Plant Cells","authors":"Sidney L Shaw;David Thoms;James Powers","doi":"10.1093/jmicro/dfy043","DOIUrl":"10.1093/jmicro/dfy043","url":null,"abstract":"The application of structured illumination approaches for super-resolution light microscopy in plant cells is reviewed. Challenges and recent inroads using both wide-field and point-based techniques are discussed with specific application to live-cell imaging. The advent of super-resolution techniques in biological microscopy has opened new frontiers for exploring the molecular distribution of proteins and small molecules in cells. Improvements in optical design and innovations in the approaches for the collection of fluorescence emission have produced substantial gains in signal from chemical labels and fluorescent proteins. Structuring the illumination to elicit fluorescence from specific or even random patterns allows the extraction of higher order spatial frequencies from specimens labeled with conventional probes. Application of this approach to plant systems for super-resolution imaging has been relatively slow owing in large part to aberrations incurred when imaging through the plant cell wall. In this brief review, we address the use of two prominent methods for generating super-resolution images in living plant specimens and discuss future directions for gaining better access to these techniques.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":"68 1","pages":"37-44"},"PeriodicalIF":1.8,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfy043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36554902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Three-dimensional ultrastructure and hyperspectral imaging of metabolite accumulation and dynamics in Haematococcus and Chlorella 红球藻和小球藻代谢产物积累和动力学的三维超微结构和高光谱成像

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI: 10.1093/jmicro/dfy142

Shuhei Ota;Shigeyuki Kawano

{"title":"Three-dimensional ultrastructure and hyperspectral imaging of metabolite accumulation and dynamics in Haematococcus and Chlorella","authors":"Shuhei Ota;Shigeyuki Kawano","doi":"10.1093/jmicro/dfy142","DOIUrl":"10.1093/jmicro/dfy142","url":null,"abstract":"Basic as well as applied phycological researches have developed alongside light and electron microscopy. This article reviews recent studies of bioimaging in the context of algal biorefining, and explains how 3D-TEM imaging are becoming useful tools for analyzing and monitoring the dynamics of algal cells. Phycology has developed alongside light and electron microscopy techniques. Since the 1950s, progress in the field has accelerated dramatically with the advent of electron microscopy. Transmission electron microscopes can only acquire imaging data on a 2D plane. Currently, many of the life sciences are seeking to obtain 3D images with electron microscopy for the accurate interpretation of subcellular dynamics. Three-dimensional reconstruction using serial sections is a method that can cover relatively large cells or tissues without requiring special equipment. Another challenge is monitoring secondary metabolites (such as lipids or carotenoids) in intact cells. This became feasible with hyperspectral cameras, which enable the acquisition of wide-range spectral information in living cells. Here, we review bioimaging studies on the intracellular dynamics of substances such as lipids, carotenoids and phosphorus using conventional to state-of-the-art microscopy techniques in the field of algal biorefining.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":"68 1","pages":"57-68"},"PeriodicalIF":1.8,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfy142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36848759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Electron tomography in plant cell biology 植物细胞生物学中的电子断层扫描

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI: 10.1093/jmicro/dfy133

Marisa S Otegui;Jannice G Pennington

引用次数: 19

Long-term live-cell imaging approaches to study lateral root formation in Arabidopsis thaliana 拟南芥侧根形成的长期活细胞成像研究

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI: 10.1093/jmicro/dfy135

Tatsuaki Goh

{"title":"Long-term live-cell imaging approaches to study lateral root formation in Arabidopsis thaliana","authors":"Tatsuaki Goh","doi":"10.1093/jmicro/dfy135","DOIUrl":"10.1093/jmicro/dfy135","url":null,"abstract":"Observing cellular and molecular processes for an extended time at various scales are crucial for understanding biological processes during organogenesis. This review focuses on the contribution of multi-scale imaging approaches to understanding plant lateral root formation processes from cells to organs. Lateral roots comprise the majority of the branching root system and are important for acquiring nutrients and water from soil in addition to providing anchorage. Lateral roots develop post-embryonically from existing root parts and originate from a subset of specified pericycle cells (lateral root founder cells) located deep inside roots. Small numbers of these specified pericycle cells undergo several rounds of cell division to create a dome-shaped primordium, which eventually organizes a meristem, an essential region for plant growth with active cell division, and emerges from its parental root as a lateral root. Observing cellular and molecular processes for an extended time at various scales are crucial for understanding biological processes during organogenesis. Lateral root formation is an example of the successful application of live-cell imaging approaches to understand various aspects of developmental events in plants, including cell fate determination, cell proliferation, cell-to-cell interaction and cell wall modification. Here I review the recent progress in understanding the molecular mechanisms of lateral root formation and the contribution of live-cell imaging approaches.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":"68 1","pages":"4-12"},"PeriodicalIF":1.8,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfy135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36706449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Microscopy in 2019 with a renewed editorial board 2019年Microscopy与更新的编委会

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI: 10.1093/jmicro/dfz005

Shigeo Okabe

引用次数: 0

For Microscopy special issue on ‘Plant Science’ 显微镜“植物科学”特刊

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI: 10.1093/jmicro/dfz006

Yoshinobu Mineyuki;Marisa S Otegui

引用次数: 2

How do plastids and mitochondria divide? 质体和线粒体是如何分裂的？

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI: 10.1093/jmicro/dfy132

Yamato Yoshida;Yuko Mogi

{"title":"How do plastids and mitochondria divide?","authors":"Yamato Yoshida;Yuko Mogi","doi":"10.1093/jmicro/dfy132","DOIUrl":"10.1093/jmicro/dfy132","url":null,"abstract":"Plastids and mitochondria do not multiply de novo but though the division of pre-existing organelles. Here, we review the structural frameworks of the mechanisms of plastid and mitochondrial division. Then, we highlight fundamental issues that need to be resolved to reveal the underlying mechanisms of plastid and mitochondrial division. Plastids and mitochondria are thought to have originated from free-living cyanobacterial and alpha-proteobacterial ancestors, respectively, via endosymbiosis. Their evolutionary origins dictate that these organelles do not multiply de novo but through the division of pre-existing plastids and mitochondria. Over the past three decades, studies have shown that plastid and mitochondrial division are performed by contractile ring-shaped structures, broadly termed the plastid and mitochondrial-division machineries. Interestingly, the division machineries are hybrid forms of the bacterial cell division system and eukaryotic membrane fission system. The structure and function of the plastid and mitochondrial-division machineries are similar to each other, implying that the division machineries evolved in parallel since their establishment in primitive eukaryotes. Compared with our knowledge of their structures, our understanding of the mechanical details of how these division machineries function is still quite limited. Here, we review and compare the structural frameworks of the plastid and mitochondrial-division machineries in both lower and higher eukaryotes. Then, we highlight fundamental issues that need to be resolved to reveal the underlying mechanisms of plastid and mitochondrial division. Finally, we highlight related studies that point to an exciting future for the field.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":"68 1","pages":"45-56"},"PeriodicalIF":1.8,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfy132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36766106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Light-dependent spatiotemporal control of plant cell development and organelle movement in fern gametophytes 蕨类配子体植物细胞发育和细胞器运动的光依赖时空控制

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI: 10.1093/jmicro/dfy143

Masamitsu Wada

{"title":"Light-dependent spatiotemporal control of plant cell development and organelle movement in fern gametophytes","authors":"Masamitsu Wada","doi":"10.1093/jmicro/dfy143","DOIUrl":"10.1093/jmicro/dfy143","url":null,"abstract":"The haploid gametophyte generation of ferns is an excellent experimental material for cell biology studies because of its simple structure and high sensitivity to light. Each step of the developmental process, such as cell growth, cell cycle and the direction of cell division, is controlled, step by step, by light, unlike what happens in complex seed plant tissues. To perform analyses at the cell or organelle level, we have developed special tools, instruments and techniques, such as a cuvette suitable for repeated centrifugation in particular directions, microbeam irradiators for partial cell irradiation and single-cell ligation technique to create enucleated cells. Some of our main discoveries are as follows: (1) changes in the intracellular position of the nucleus in long protonemal cells by centrifugation revealed that the nuclear position or a factor(s) that is/are co-centrifuged with the nucleus is important for the decision regarding the place of the formation of preprophase bands and the timing of their disappearance, which determines the position where the new cell wall attaches to the mother cell wall; (2) even within a single cell, various phenomena could be induced by blue or red light, with the localization of the blue or red light receptors being different depending on the phenomenon; (3) de novo mRNA synthesis is not involved in the signal transduction pathways underlying light-induced chloroplast movements. In this review article, various microscopic techniques, in addition to the results of physiology studies in fern gametophytes, are described.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":"68 1","pages":"13-36"},"PeriodicalIF":1.8,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfy143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36805305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1