{"title":"FIB/SEM断层扫描阐明牙骨质和牙周膜的细胞网络","authors":"Shingo Hirashima;Keisuke Ohta;Tomonoshin Kanazawa;Akinobu Togo;Risa Tsuneyoshi;Jingo Kusukawa;Kei-ichiro Nakamura","doi":"10.1093/jmicro/dfz117","DOIUrl":null,"url":null,"abstract":"Cementocytes in cementum form a lacuna-canalicular network. However, the 3D ultrastructure and range of the cementocyte network are unclear. Here, the 3D ultrastructure of the cementocyte network at the interface between cementum and periodontal ligament (PDL) was investigated on the mesoscale using FIB/SEM tomography. The results revealed a cellular network of cementocytes and PDL cells. A previous histomorphological study revealed the osteocyte-osteoblast-PDL cellular network. We extended this knowledge and revealed the cementum-PDL-bone cellular network, which may orchestrate the remodeling and modification of periodontal tissue, using a suitable method for imaging of complex tissue. The 3D ultrastructure of the cementocyte architecture around the interface between cementum and periodontal ligament (PDL) was investigated using FIB/SEM tomography. As a result, we showed a cellular interconnection between cementocytes and PDL cells and revealed the cementum-PDL-bone cellular network, extending our previous morphological discovery of the osteocyte-osteoblast-PDL cellular network.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":"69 1","pages":"53-58"},"PeriodicalIF":1.8000,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz117","citationCount":"8","resultStr":"{\"title\":\"Cellular network across cementum and periodontal ligament elucidated by FIB/SEM tomography\",\"authors\":\"Shingo Hirashima;Keisuke Ohta;Tomonoshin Kanazawa;Akinobu Togo;Risa Tsuneyoshi;Jingo Kusukawa;Kei-ichiro Nakamura\",\"doi\":\"10.1093/jmicro/dfz117\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Cementocytes in cementum form a lacuna-canalicular network. However, the 3D ultrastructure and range of the cementocyte network are unclear. Here, the 3D ultrastructure of the cementocyte network at the interface between cementum and periodontal ligament (PDL) was investigated on the mesoscale using FIB/SEM tomography. The results revealed a cellular network of cementocytes and PDL cells. A previous histomorphological study revealed the osteocyte-osteoblast-PDL cellular network. We extended this knowledge and revealed the cementum-PDL-bone cellular network, which may orchestrate the remodeling and modification of periodontal tissue, using a suitable method for imaging of complex tissue. The 3D ultrastructure of the cementocyte architecture around the interface between cementum and periodontal ligament (PDL) was investigated using FIB/SEM tomography. As a result, we showed a cellular interconnection between cementocytes and PDL cells and revealed the cementum-PDL-bone cellular network, extending our previous morphological discovery of the osteocyte-osteoblast-PDL cellular network.\",\"PeriodicalId\":18515,\"journal\":{\"name\":\"Microscopy\",\"volume\":\"69 1\",\"pages\":\"53-58\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2019-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1093/jmicro/dfz117\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microscopy\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://ieeexplore.ieee.org/document/9108466/\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopy","FirstCategoryId":"5","ListUrlMain":"https://ieeexplore.ieee.org/document/9108466/","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cellular network across cementum and periodontal ligament elucidated by FIB/SEM tomography
Cementocytes in cementum form a lacuna-canalicular network. However, the 3D ultrastructure and range of the cementocyte network are unclear. Here, the 3D ultrastructure of the cementocyte network at the interface between cementum and periodontal ligament (PDL) was investigated on the mesoscale using FIB/SEM tomography. The results revealed a cellular network of cementocytes and PDL cells. A previous histomorphological study revealed the osteocyte-osteoblast-PDL cellular network. We extended this knowledge and revealed the cementum-PDL-bone cellular network, which may orchestrate the remodeling and modification of periodontal tissue, using a suitable method for imaging of complex tissue. The 3D ultrastructure of the cementocyte architecture around the interface between cementum and periodontal ligament (PDL) was investigated using FIB/SEM tomography. As a result, we showed a cellular interconnection between cementocytes and PDL cells and revealed the cementum-PDL-bone cellular network, extending our previous morphological discovery of the osteocyte-osteoblast-PDL cellular network.
期刊介绍:
Microscopy, previously Journal of Electron Microscopy, promotes research combined with any type of microscopy techniques, applied in life and material sciences. Microscopy is the official journal of the Japanese Society of Microscopy.