Cellular network across cementum and periodontal ligament elucidated by FIB/SEM tomography

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-11-01 DOI:10.1093/jmicro/dfz117

Shingo Hirashima;Keisuke Ohta;Tomonoshin Kanazawa;Akinobu Togo;Risa Tsuneyoshi;Jingo Kusukawa;Kei-ichiro Nakamura

{"title":"Cellular network across cementum and periodontal ligament elucidated by FIB/SEM tomography","authors":"Shingo Hirashima;Keisuke Ohta;Tomonoshin Kanazawa;Akinobu Togo;Risa Tsuneyoshi;Jingo Kusukawa;Kei-ichiro Nakamura","doi":"10.1093/jmicro/dfz117","DOIUrl":null,"url":null,"abstract":"Cementocytes in cementum form a lacuna-canalicular network. However, the 3D ultrastructure and range of the cementocyte network are unclear. Here, the 3D ultrastructure of the cementocyte network at the interface between cementum and periodontal ligament (PDL) was investigated on the mesoscale using FIB/SEM tomography. The results revealed a cellular network of cementocytes and PDL cells. A previous histomorphological study revealed the osteocyte-osteoblast-PDL cellular network. We extended this knowledge and revealed the cementum-PDL-bone cellular network, which may orchestrate the remodeling and modification of periodontal tissue, using a suitable method for imaging of complex tissue. The 3D ultrastructure of the cementocyte architecture around the interface between cementum and periodontal ligament (PDL) was investigated using FIB/SEM tomography. As a result, we showed a cellular interconnection between cementocytes and PDL cells and revealed the cementum-PDL-bone cellular network, extending our previous morphological discovery of the osteocyte-osteoblast-PDL cellular network.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfz117","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopy","FirstCategoryId":"5","ListUrlMain":"https://ieeexplore.ieee.org/document/9108466/","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 8

Abstract

Cementocytes in cementum form a lacuna-canalicular network. However, the 3D ultrastructure and range of the cementocyte network are unclear. Here, the 3D ultrastructure of the cementocyte network at the interface between cementum and periodontal ligament (PDL) was investigated on the mesoscale using FIB/SEM tomography. The results revealed a cellular network of cementocytes and PDL cells. A previous histomorphological study revealed the osteocyte-osteoblast-PDL cellular network. We extended this knowledge and revealed the cementum-PDL-bone cellular network, which may orchestrate the remodeling and modification of periodontal tissue, using a suitable method for imaging of complex tissue. The 3D ultrastructure of the cementocyte architecture around the interface between cementum and periodontal ligament (PDL) was investigated using FIB/SEM tomography. As a result, we showed a cellular interconnection between cementocytes and PDL cells and revealed the cementum-PDL-bone cellular network, extending our previous morphological discovery of the osteocyte-osteoblast-PDL cellular network.

查看原文本刊更多论文

FIB/SEM断层扫描阐明牙骨质和牙周膜的细胞网络

牙骨质中的骨水泥细胞形成腔隙性小管网络。然而，牙骨质细胞网络的三维超微结构和范围尚不清楚。在此，使用FIB/SEM断层扫描在中尺度上研究了牙骨质和牙周膜（PDL）界面处牙骨质细胞网络的3D超微结构。结果显示了一个由牙骨质细胞和PDL细胞组成的细胞网络。先前的一项组织形态学研究揭示了骨细胞-成骨细胞PDL细胞网络。我们扩展了这一知识，并使用一种合适的复杂组织成像方法揭示了牙骨质PDL骨细胞网络，它可能协调牙周组织的重塑和修饰。利用FIB/SEM断层扫描技术研究了牙骨质与牙周膜（PDL）界面周围牙骨质细胞结构的三维超微结构。因此，我们显示了牙骨质细胞和PDL细胞之间的细胞互连，并揭示了牙骨质PDL骨细胞网络，扩展了我们之前对骨细胞-成骨细胞PDL细胞网络的形态学发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microscopy 工程技术-显微镜技术

自引率

11.10%

发文量

审稿时长

>12 weeks

期刊介绍： Microscopy, previously Journal of Electron Microscopy, promotes research combined with any type of microscopy techniques, applied in life and material sciences. Microscopy is the official journal of the Japanese Society of Microscopy.