Microscopy最新文献_第2页

Retraction to: Self-assembly of nickel icosahedrons and truncated octahedral nanocrystals on a SrTiO3 (111) support 镍二十面体和截头八面体纳米晶体在SrTiO3（111）载体上的自组装

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfab013

Atif Rasheed

{"title":"Retraction to: Self-assembly of nickel icosahedrons and truncated octahedral nanocrystals on a SrTiO3 (111) support","authors":"Atif Rasheed","doi":"10.1093/jmicro/dfab013","DOIUrl":"10.1093/jmicro/dfab013","url":null,"abstract":"Liquid cell transmission electron microscopy (LCTEM) enables imaging of dynamic processes in liquid with high spatial and temporal resolution. The widely used liquid cell (LC) consists of two stacking microchips with a thin wet sample sandwiched between them. The vertically overlapped electron-transparent membrane windows on the microchips provide passage for the electron beam. However, microchips with imprecise dimensions usually cause poor alignment of the windows and difficulty in acquiring high-quality images. In this study, we developed a new and efficient microchip fabrication process for LCTEM with a large viewing area (180 µm × 40 µm) and evaluated the resultant LC. The new positioning reference marks on the surface of the Si wafer dramatically improve the precision of dicing the wafer, making it possible to accurately align the windows on two stacking microchips. The precise alignment led to a liquid thickness of 125.6 nm close to the edge of the viewing area. The performance of our LC was demonstrated by in situ transmission electron microscopy imaging of the dynamic motions of 2-nm Pt particles. This versatile and cost-effective microchip production method can be used to fabricate other types of microchips for in situ electron microscopy.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfab013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39293320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of cell volume using in-line digital holography 在线数字全息术测量细胞体积

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfaa077

Hangjian Ling;Kaushik Sridhar;Sumanth Gollapudi;Jyoti Kumar;Robert S Ohgami

引用次数: 1

A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging 一种可光切换的荧光蛋白的小时延时和亚秒分辨率超分辨率成像

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfab001

Tetsuichi Wazawa;Ryohei Noma;Shusaku Uto;Kazunori Sugiura;Takashi Washio;Takeharu Nagai

{"title":"A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging","authors":"Tetsuichi Wazawa;Ryohei Noma;Shusaku Uto;Kazunori Sugiura;Takashi Washio;Takeharu Nagai","doi":"10.1093/jmicro/dfab001","DOIUrl":"10.1093/jmicro/dfab001","url":null,"abstract":"Reversibly photoswitchable fluorescent proteins (RSFPs) are a class of fluorescent proteins whose fluorescence can be turned on and off by light irradiation. RSFPs have become essential tools for super-resolution (SR) imaging. Because most SR imaging techniques require high-power-density illumination, mitigating phototoxicity in cells due to intense light irradiation has been a challenge. Although we previously developed an RSFP named Kohinoor to achieve SR imaging with low phototoxicity, the photoproperties were insufficient to move a step further to explore the cellular dynamics by SR imaging. Here, we show an improved version of RSFP, Kohinoor2.0, which is suitable for SR imaging of cellular processes. Kohinoor2.0 shows a 2.6-fold higher fluorescence intensity, 2.5-fold faster chromophore maturation and 1.5-fold faster off-switching than Kohinoor. The analysis of the pH dependence of the visible absorption band revealed that Kohinoor2.0 and Kohinoor were in equilibria among multiple fluorescently bright and dark states, with the mutations introduced into Kohinoor2.0 bringing about a higher stabilization of the fluorescently bright states compared to Kohinoor. Using Kohinoor2.0 with our SR imaging technique, super-resolution polarization demodulation/on-state polarization angle narrowing, we conducted 4-h time-lapse SR imaging of an actin filament network in mammalian cells with a total acquisition time of 480 s without a noticeable indication of phototoxicity. Furthermore, we demonstrated the SR imaging of mitochondria dynamics at a time resolution of 0.5 s, in which the fusion and fission processes were clearly visualized. Thus, Kohinoor2.0 is shown to be an invaluable RSFP for the SR imaging of cellular dynamics.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfab001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38768950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Application of deproteinized bovine bone mineral as proangiogenic scaffold for alveolar bone formation in beagle dogs 脱蛋白牛骨矿物质作为促血管生成支架在比格犬牙槽骨形成中的应用

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfab007

Keita Ogasawara;Masahiro To;Yu-Hao Liu;Toshimitsu Okudera;Takatsuna Nakamura;Masato Matsuo

{"title":"Application of deproteinized bovine bone mineral as proangiogenic scaffold for alveolar bone formation in beagle dogs","authors":"Keita Ogasawara;Masahiro To;Yu-Hao Liu;Toshimitsu Okudera;Takatsuna Nakamura;Masato Matsuo","doi":"10.1093/jmicro/dfab007","DOIUrl":"10.1093/jmicro/dfab007","url":null,"abstract":"Alveolar bone repair after tooth extraction is essential after oral surgeries. Various grafting materials are used to promote the regeneration of lost alveolar bone. This study analysed the morphological features of the tissue regeneration process using deproteinized bovine bone mineral (DBBM). DBBM was used to densely fill the extraction sockets in beagle dogs. Following resin casting of the vasculature, stereomicroscopy and scanning electron microscopy were used to observe blood vessels and hard tissues in haematoxylin and eosin-stained sections on postoperative days 14, 30 and 90 in conjunction with vascular endothelial growth factor (VEGF) immunostaining to evaluate alveolar bone vascularization. On day 14 post-operation, the DBBM granules tightly filled the extraction sockets, maintained alveolar margin height and formed a scaffold for aiding angiogenesis and new bone formation. On day 30, new bone formation was observed around the DBBM granules. By day 90, bone tissue regeneration progressed in both groups but was more pronounced in the DBBM group. Alveolar margin height was maintained in the DBBM group throughout the study. Furthermore, VEGF expression in the DBBM group was detected around newly formed bone. We conclude that DBBM acts as a suitable scaffold for new bone generation, as well as angiogenesis around healing alveolar bone, and that it has the potential to play a key role in vascularization and bone formation.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfab007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25322188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Fabrication of a liquid cell for in situ transmission electron microscopy 用于原位透射电子显微镜的液体细胞的制备

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfaa076

Xiaoguang Li;Kazutaka Mitsuishi;Masaki Takeguchi

{"title":"Fabrication of a liquid cell for in situ transmission electron microscopy","authors":"Xiaoguang Li;Kazutaka Mitsuishi;Masaki Takeguchi","doi":"10.1093/jmicro/dfaa076","DOIUrl":"10.1093/jmicro/dfaa076","url":null,"abstract":"Liquid cell transmission electron microscopy (LCTEM) enables imaging of dynamic processes in liquid with high spatial and temporal resolution. The widely used liquid cell (LC) consists of two stacking microchips with a thin wet sample sandwiched between them. The vertically overlapped electron-transparent membrane windows on the microchips provide passage for the electron beam. However, microchips with imprecise dimensions usually cause poor alignment of the windows and difficulty in acquiring high-quality images. In this study, we developed a new and efficient microchip fabrication process for LCTEM with a large viewing area (180 µm × 40 µm) and evaluated the resultant LC. The new positioning reference marks on the surface of the Si wafer dramatically improve the precision of dicing the wafer, making it possible to accurately align the windows on two stacking microchips. The precise alignment led to a liquid thickness of 125.6 nm close to the edge of the viewing area. The performance of our LC was demonstrated by in situ transmission electron microscopy imaging of the dynamic motions of 2-nm Pt particles. This versatile and cost-effective microchip production method can be used to fabricate other types of microchips for in situ electron microscopy.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfaa076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38711357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Self-assembly of nickel icosahedrons and truncated octahedral nanocrystals on a SrTiO3 (111) support 镍二十面体和截头八面体纳米晶体在SrTiO3（111）载体上的自组装

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfaa078

Atif Rasheed

{"title":"Self-assembly of nickel icosahedrons and truncated octahedral nanocrystals on a SrTiO3 (111) support","authors":"Atif Rasheed","doi":"10.1093/jmicro/dfaa078","DOIUrl":"10.1093/jmicro/dfaa078","url":null,"abstract":"Nickel nanocrystals have received much attention for their ferromagnetic properties. The crystal properties are strongly dependent on their facets and therefore detailed study of their morphology, facets and orientation is critical for magnetic applications. In this work, equilibrium crystal shapes of self-assembled nickel nanocrystals on the (111) termination of strontium titanate (SrTiO\u0000<inf>3</inf>\u0000) at room temperature and under ultra-high vacuum (UHV) conditions have been investigated using scanning tunneling microscope. SrTiO\u0000<inf>3</inf>\u0000 (111) substrate was sputtered (0.5 keV, 2.5 µA, 10 min) and annealed (900°C, 1 h) under UHV conditions. Three different periodicities were observed: 2.21 ± 0.01 nm corresponding to (4 × 4) reconstruction, 3.31 ± 0.02 nm corresponding to (6 × 6) reconstruction and 2.85 ± 0.05 nm, rotated at 30° with respect to (4 × 4) reconstruction, corresponding to (3√3 × 3√3)R30° reconstruction. Nickel (∼1 ml) was deposited using an e-beam evaporator on the substrate preheated to 320°C and the sample was post-annealed multiple times. Nickel took platonic shapes of supported icosahedron comprising of (111) facets and truncated octahedron comprising of (001) and (111) facets. Based on surface energy ratios of truncated octahedrons at equilibrium, the work of adhesion was calculated to be 3.889 ± 0.167 J/m\u00002\u0000.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfaa078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38759348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imaging simulation of charged nanowires in TEM with large defocus distance 带电纳米线在大散焦距TEM中的成像模拟

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfab008

Te Shi;Shikai Liu;H Tian;Z J Ding

引用次数: 1

Evaluation of TEM specimen quality prepared by focused ion beam using symmetry breaking index of convergent-beam electron diffraction 用会聚束电子衍射对称破坏指数评价聚焦离子束制备TEM样品的质量

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfab002

Daisuke Morikawa;Masaki Ageishi;Kaori Sato;Kenji Tsuda;Masami Terauchi

引用次数: 0

Damage-less observation of polymers by electron dose control in scanning electron microscope 扫描电子显微镜中电子剂量控制对聚合物的无损伤观察

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfab006

Yoichiro Hashimoto;Kunji Shigeto;Ryo Komatsuzaki;Tsutomu Saito;Takashi Sekiguchi

引用次数: 3

A simple preparation method for CLEM using pre-embedding immunohistochemistry with a novel fluorescent probe and stable embedding resin 新型荧光探针和稳定包埋树脂预包埋免疫组化制备CLEM的简便方法

IF 1.8 4区工程技术

Microscopy Pub Date : 2021-08-01 DOI: 10.1093/jmicro/dfab005

Takaaki Kanemaru;Teruyoshi Kondo;Kei-ichiro Nakamura;Hiroyuki Morimoto;Kentaro Nishi;Shin-ichiro Isobe

{"title":"A simple preparation method for CLEM using pre-embedding immunohistochemistry with a novel fluorescent probe and stable embedding resin","authors":"Takaaki Kanemaru;Teruyoshi Kondo;Kei-ichiro Nakamura;Hiroyuki Morimoto;Kentaro Nishi;Shin-ichiro Isobe","doi":"10.1093/jmicro/dfab005","DOIUrl":"10.1093/jmicro/dfab005","url":null,"abstract":"Correlative light and electron microscopy (CLEM) is an excellent approach for examining the cellular localization of biomolecules. Here, we developed a simple method for CLEM by combining pre-embedding immunohistochemistry with a novel fluorescent probe, namely Fluolid NS Orange, and an embedding resin called ʻDurcupan\u0000™\u0000ʼ. Specimens were embedded in Durcupan\u0000™\u0000 or LR White after immunolabeling and post-fixation using glutaraldehyde and osmium tetroxide. Next, ultrathin sections were prepared on a finder grid with navigation markers. The section of the specimen embedded in Durcupan\u0000™\u0000 was found to be more stable against electron beam irradiation than specimens embedded in LR White. A fluorescence light microscopy image and a transmission electron microscopy (TEM) image, at wide-field, and low magnification, were independently obtained with the same ultrathin section. Using the three corners between finder grid bars as landmarks, fluorescence light microscopy images were superimposed with wide-field, low-magnification TEM images to identify the region of interest, which was subsequently enlarged to ascertain cellular structures localized beneath fluorescent signals. However, the enlarged TEM images appeared blurred, and fluorescence signals had a hazy appearance. To resolve this, the enlarged TEM images were replaced by high-resolution TEM images focused directly on the region of interest, thereby facilitating the collection of high-resolution CLEM images. The simple sample processing method for CLEM using osmium-resistant Fluolid NS Orange and electron beam damage-resistant Durcupan™ allowed the determination of the precise localization of fluorescence signals at subcellular levels.","PeriodicalId":18515,"journal":{"name":"Microscopy","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/jmicro/dfab005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38866672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0