Microbes and Environments最新文献

{"title":"A Potential Indicator Gene, tetM, to Assess Contamination by Antibiotic Resistance Genes in Greenhouses in South Korea.","authors":"Seunggyun Han, Raan Shin, Song-Hee Ryu, Tatsuya Unno, Hor-Gil Hur, Hanseob Shin","doi":"10.1264/jsme2.ME24053","DOIUrl":"10.1264/jsme2.ME24053","url":null,"abstract":"<p><p>Antibiotic resistance genes (ARGs) have been emerging as a concerning threat to both environment and public health. The continuous input of manure, irrigation water, and fertilizers increases the abundance of ARGs in agricultural environments. However, current risk assessments have focused on clinical settings, which are not applicable to environmental settings. Therefore, we herein aimed to identify and assess indicator genes to reduce the time and effort required for ARG surveillance. A nationwide ana-lysis of 322 ARGs and 58 mobile genetic elements (MGEs) was performed on 42 greenhouse and 19 control soil samples. The chemical properties and pH of soil were also investigated to characterize differences between greenhouse and control soil samples. The results obtained showed that the abundance of ARGS was significantly higher and ion concentrations were higher in greenhouse samples than in control samples. These results indicate that agricultural activities increased the abundance of ARGs. Furthermore, the abundance of core genes was significantly higher in greenhouse samples than in control samples, and the chemical characteristics of soil significantly differed between these samples. Among the discriminatory genes selected, tetM was identified as an ARG surveillance indicator gene based on its clinical relevance, prevalence in the soil resistome, and relationship with mobile genetic elements. The present results will contribute to the continuous and rapid surveillance of antibiotic resistance dissemination and proliferation in greenhouses in South Korea.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"39 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11821766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gustatory Responsiveness of Honey Bees Colonized with a Defined or Conventional Gut Microbiota.","authors":"Shota Suenami, Masato Sato, Ryo Miyazaki","doi":"10.1264/jsme2.ME23081","DOIUrl":"10.1264/jsme2.ME23081","url":null,"abstract":"<p><p>Gut microbes have many beneficial functions for host animals, such as food digestion and development of the immune system. An increasing number of studies report that gut bacteria also affect host neural function and behavior. The sucrose responsiveness of the western honey bee Apis mellifera, which harbors a characteristic gut microbiota, was recently reported to be increased by the presence of gut microbes. However, this responsiveness may vary depending on the experimental design, as animal behavior may be modulated by physiological states and environmental conditions. To evaluate the robustness of the effects of the gut microbiota on host gustatory responsiveness, we herein examined the sucrose responsiveness of honey bees colonized with a defined bacterial community or a conventional gut microbiota extracted from a field-collected bee. Although colonization was experimentally verified, sucrose responsiveness did not significantly differ among treatments after the 2- or 5-h starvation period. We concluded that the sucrose responsiveness of A. mellifera is not always affected by its gut microbiota. Therefore, host physiological conditions and environmental factors need to be considered when evaluating the impact of the gut microbiota on host neural function and behavior.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"39 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10982108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Reduced Complementary Genomes of Dual Bacterial Symbionts in the Mulberry Psyllid Anomoneura mori.","authors":"Yuka Yasuda, Hiromitsu Inoue, Yuu Hirose, Atsushi Nakabachi","doi":"10.1264/jsme2.ME24041","DOIUrl":"10.1264/jsme2.ME24041","url":null,"abstract":"<p><p>The genomes of obligately host-restricted bacteria suffer from accumulating mildly deleterious mutations, resulting in marked size reductions. Psyllids (Hemiptera) are phloem sap-sucking insects with a specialized organ called the bacteriome, which typically harbors two vertically transmitted bacterial symbionts: the primary symbiont \"Candidatus Carsonella ruddii\" (Gammaproteobacteria) and a secondary symbiont that is phylogenetically diverse among psyllid lineages. The genomes of several Carsonella lineages were revealed to be markedly reduced (158-174‍ ‍kb), AT-rich (14.0-17.9% GC), and structurally conserved with similar gene inventories devoted to synthesizing essential amino acids that are scarce in the phloem sap. However, limited genomic information is currently available on secondary symbionts. Therefore, the present study investigated the genomes of the bacteriome-associated dual symbionts, Secondary_AM (Gammaproteobacteria) and Carsonella_AM, in the mulberry psyllid Anomoneura mori (Psyllidae). The results obtained revealed that the Secondary_AM genome is as small and AT-rich (229,822 bp, 17.3% GC) as those of Carsonella lineages, including Carsonella_AM (169,120 bp, 16.2% GC), implying that Secondary_AM is an evolutionarily ancient obligate mutualist, as is Carsonella. Phylogenomic ana-lyses showed that Secondary_AM is sister to \"Candidatus Psyllophila symbiotica\" of Cacopsylla spp. (Psyllidae), the genomes of which were recently reported (221-237‍ ‍kb, 17.3-18.6% GC). The Secondary_AM and Psyllophila genomes showed highly conserved synteny, sharing all genes for complementing the incomplete tryptophan biosynthetic pathway of Carsonella and those for synthesizing B vitamins. However, sulfur assimilation and carotenoid-synthesizing genes were only retained in Secondary_AM and Psyllophila, respectively, indicating ongoing gene silencing. Average nucleotide identity, gene ortholog similarity, genome-wide synteny, and substitution rates suggest that the Secondary_AM/Psyllophila genomes are more labile than Carsonella genomes.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"39 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quest for Nitrous Oxide-reducing Bacteria Present in an Anammox Biofilm Fed with Nitrous Oxide.","authors":"Kohei Oba, Toshikazu Suenaga, Shohei Yasuda, Megumi Kuroiwa, Tomoyuki Hori, Susanne Lackner, Akihiko Terada","doi":"10.1264/jsme2.ME23106","DOIUrl":"10.1264/jsme2.ME23106","url":null,"abstract":"<p><p>N₂O-reducing bacteria have been examined and harnessed to develop technologies that reduce the emission of N₂O, a greenhouse gas produced by biological nitrogen removal. Recent investigations using omics and physiological activity approaches have revealed the ecophysiologies of these bacteria during nitrogen removal. Nevertheless, their involvement in‍ ‍anammox processes remain unclear. Therefore, the present study investigated the identity, genetic potential, and activity‍ ‍of N₂O reducers in an anammox reactor. We hypothesized that N₂O is limiting for N₂O-reducing bacteria‍ ‍and an‍ ‍exogeneous N₂O supply enriches as-yet-uncultured N₂O-reducing bacteria. We conducted a 1200-day incubation of N₂O-reducing bacteria in an anammox consortium using gas-permeable membrane biofilm reactors (MBfRs), which efficiently supply N₂O in a bubbleless form directly to a biofilm grown on a gas-permeable membrane. A ¹⁵N tracer test indicated that the supply of N₂O resulted in an enriched biomass with a higher N₂O sink potential. Quantitative PCR and 16S rRNA amplicon sequencing revealed Clade II nosZ type-carrying N₂O-reducing bacteria as protagonists of N₂O sinks. Shotgun metagenomics showed the genetic potentials of the predominant Clade II nosZ-carrying bacteria, Anaerolineae and Ignavibacteria in MBfRs. Gemmatimonadota and non-anammox Planctomycetota increased their abundance in MBfRs despite their overall lower abundance. The implication of N₂O as an inhibitory compound scavenging vitamin B12, which is essential for the synthesis of methionine, suggested its limited suppressive effect on the growth of B12-dependent bacteria, including N₂O reducers. We identified Dehalococcoidia and Clostridia as predominant N₂O sinks in an anammox consortium fed exogenous N₂O because of the higher metabolic potential of vitamin B12-dependent biosynthesis.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"39 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10982107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140306120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Soil Antagonism against the White Root Rot Fungus Rosellinia necatrix and Pathogen Mycosphere Communities in Biochar-amended Soil.","authors":"Yong Guo, Sachie Horii, Satoko Kanematsu","doi":"10.1264/jsme2.ME24060","DOIUrl":"10.1264/jsme2.ME24060","url":null,"abstract":"<p><p>White root rot disease caused by Rosellinia necatrix is a growing issue in orchards, and biochar pyrolyzed from the pruned branch residues of fruit trees has potential as a soil amendment agent with a number of benefits, such as long-term carbon sequestration. However, the effects of pruned branch biochar on white root rot disease remain unclear. Therefore, we compared direct antagonism against R. necatrix between soils with and without pruned pear branch biochar using a toothpick method and then linked soil physicochemical properties and microbial communities with soil antagonism. The results obtained showed that soil antagonism against the pathogen, that is, the extinction zone of R. necatrix in mycelial toothpicks, decreased in soils amended with 20% (v/v) pruned branch biochar. Soil pH was neutralized and aeration was promoted by the biochar amendment, which may be favorable for pathogen growth. An investigation of microbial communities surrounding R. necatrix mycelia indicated that antagonistic fungi affiliated with Chaetomiaceae and Trichoderma were selectively excluded from the mycosphere community in biochar-amended soil. Therefore, the enrichment of these indigenous antagonistic fungi may be important for controlling R. necatrix. Based on the present results, we do not recommend the application of pruned branch biochar to the soil area associated with the roots of fruit trees in order to avoid increasing the risk of white root rot in orchards.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"39 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11821764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Genomic Epidemiology of Pathogens to Farmed Yellowtail Fish Mycobacteriosis in Kyushu, Japan.","authors":"Takayuki Wada, Shiomi Yoshida, Takeshi Yamamoto, Lisa Nonaka, Yukari Fukushima, Chie Nakajima, Yasuhiko Suzuki, Masayuki Imajoh","doi":"10.1264/jsme2.ME24011","DOIUrl":"10.1264/jsme2.ME24011","url":null,"abstract":"<p><p>To investigate mycobacterial cases of farmed yellowtail fish in coastal areas of western Japan (Kagoshima, Kyushu), where aquaculture fisheries are active, Mycobacterium pseudoshottsii, the causative agent, was isolated from six neighboring fishing ports in 2012 and 2013. A phylogenetic ana-lysis revealed that the strains isolated from one fishing port were closely related to those isolated from other regions of Japan, suggesting the nationwide spread of a single strain. However, strains from Japan were phylogenetically distinct from those from the Mediterranean and the United States; therefore, worldwide transmission was not observed based on the limited data obtained on the strains exami-ned in this study. The present results demonstrate that a bacterial genomic ana-lysis of infected cases, a mole-cular epidemiology strategy for public health, provides useful data for estimating the prevalence and transmission pathways of M. pseudoshottsii in farmed fish. A bacterial genome ana-lysis of strains, such as that performed herein, may play an important role in monitoring the prevalence of this pathogen in fish farms and possible epidemics in the future as a result of international traffic, logistics, and trade in fisheries.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"39 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11220446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into Antifungal Mechanisms of Bacillus velezensis S141 against Cercospora Leaf Spot in Mungbean (V. radiata).","authors":"Pongpan Songwattana,&nbsp;Pakpoom Boonchuen,&nbsp;Pongdet Piromyou,&nbsp;Jenjira Wongdee,&nbsp;Teerana Greetatorn,&nbsp;Sukanya Inthaisong,&nbsp;Piyada Alisha Tantasawat,&nbsp;Kamonluck Teamtisong,&nbsp;Panlada Tittabutr,&nbsp;Nantakorn Boonkerd,&nbsp;Neung Teaumroong","doi":"10.1264/jsme2.ME22079","DOIUrl":"https://doi.org/10.1264/jsme2.ME22079","url":null,"abstract":"<p><p>Cercospora leaf spot (CLS) is caused by Cercospora canescens and is one of the most important diseases of mungbean (Vigna radiata). Cercospora leaf spot may result in economic loss in production areas. The present study investigated the potential of Bacillus velezensis S141 as a biocontrol agent for C. canescens PAK1 growth on culture plates. Cell-free secretions from a dual culture of S141+PAK1 inhibited fungal growth more than those from a single culture of S141. The biocontrol efficiency of S141 against Cercospora leaf spot on mungbean was then evaluated by spraying. The disease severity of Cercospora leaf spot was significantly reduced in plants treated with S141, with a control efficiency of 83% after 2 days of infection. Comparative transcriptomics and qRT-PCR ana-lyses of S141 during C. canescens inhibition were performed to elucidate the antifungal mechanisms underlying its antifungal activity against Cercospora leaf spot. According to the differentially expressed genes, most up-regulated genes involved in the biosynthetic genes encoding enzymatic hydrolases, including protease, β-glucanase, and N-acyl glucosaminase, were detected in strain S141 following its interaction. Moreover, genes related to secondary metabolites (surfactin, bacilysin, and bacillomycin D) were up-regulated. Collectively, these results suggest that S141 exhibited strong antifungal activity against C. canescens due to multiple enzymatic hydrolases and secondary metabolites. Therefore, the present study provides insights into the biological network responsible for the antifungal activity of B. velezensis S141 against C. canescens.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9365129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Simple and Effective Method for Solid Medium Cultivation of Strictly Hydrogen- and Sulfur-oxidizing Chemolithoautotrophs Predominant in Deep-‍sea Hydrothermal Fields.","authors":"Hisashi Muto, Junichi Miyazaki, Shigeki Sawayama, Ken Takai, Satoshi Nakagawa","doi":"10.1264/jsme2.ME23072","DOIUrl":"10.1264/jsme2.ME23072","url":null,"abstract":"<p><p>Strictly hydrogen- and sulfur-oxidizing chemolithoautotrophic bacteria, particularly members of the phyla Campylobacterota and Aquificota, have a cosmopolitan distribution in deep-sea hydrothermal fields. The successful cultivation of these microorganisms in liquid media has provided insights into their physiological, evolutionary, and ecological characteristics. Notably, recent population genetic studies on Sulfurimonas (Campylobacterota) and Persephonella (Aquificota) revealed geographic separation in their populations. Advances in this field of research are largely dependent on the availability of pure cultures, which demand labor-intensive liquid cultivation procedures, such as dilution-to-extinction, given the longstanding assumption that many strictly or facultatively anaerobic chemolithoautotrophs cannot easily form colonies on solid media. We herein describe a simple and cost-effective approach for cultivating these chemolithoautotrophs on solid media. The results obtained suggest that not only the choice of gelling agent, but also the gas phase composition significantly affect the colony-forming ratio of diverse laboratory strains. The use of gellan gum as a gelling agent combined with high concentrations of H₂ and CO₂ in a pouch bag promoted the formation of colonies. This contrasted with the absence of colony formation on an agar-solidified medium, in which thiosulfate served as an electron donor, nitrate as an electron acceptor, and bicarbonate as a carbon source, placed in anaerobic jars under an N₂ atmosphere. Our method efficiently isolated chemolithoautotrophs from a deep-sea vent sample, underscoring its potential value in research requiring pure cultures of hydrogen- and sulfur-oxidizing chemolithoautotrophs.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10728628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138806922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species-specific Primer and Probe Sets for Detection of Syntrophic Long-chain Fatty Acid-degrading Bacteria in Anaerobic Digestion Using Quantitative PCR.","authors":"Riku Sakurai,&nbsp;Yasuhiro Fukuda,&nbsp;Chika Tada","doi":"10.1264/jsme2.ME23023","DOIUrl":"https://doi.org/10.1264/jsme2.ME23023","url":null,"abstract":"<p><p>Lipid-rich wastes are energy-dense substrates for anaerobic digestion. However, long-chain fatty acids (LCFAs), key intermediates in lipid degradation, inhibit methanogenic activity. In this study, TaqMan-based qPCR assays targeting the 16S rRNA gene of the cardinal LCFA-degrading bacterial species Syntrophomonas palmitatica and S. zehnderi were developed and validated. A trial experiment showed the advantage of species-specific quantification versus genus-specific quantification in assessing bacterial capacity for lipidic waste degradation. These qPCR assays will serve as monitoring tools for estimating the LCFA-degrading capacity of anaerobic digester communities and developing an effective strategy to enrich LCFA-degrading bacteria.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9731833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between Rumen Microbial Composition and Fibrolytic Isozyme Activity During the Biodegradation of Rice Straw Powder Using Rumen Fluid.","authors":"Shuhei Takizawa, Ryoki Asano, Kenichi Abe, Yasuhiro Fukuda, Yasunori Baba, Riku Sakurai, Chika Tada, Yutaka Nakai","doi":"10.1264/jsme2.ME23041","DOIUrl":"10.1264/jsme2.ME23041","url":null,"abstract":"<p><p>Rumen fibrolytic microorganisms have been used to increase the rate of lignocellulosic biomass biodegradation; however, the microbial and isozymatic characteristics of biodegradation remain unclear. Therefore, the present study investigated the relationship between rumen microorganisms and fibrolytic isozymes associated with lignocellulosic biomass hydrolysis. Rice straw, a widely available agricultural byproduct, was ground and used as a substrate. The biodegradation of rice straw powder was performed anaerobically in rumen fluid for 48 h. The results obtained revealed that 31.6 and 23.3% of cellulose and hemicellulose, respectively, were degraded. The total concentration of volatile fatty acids showed a 1.8-fold increase (from 85.4 to 151.6‍ ‍mM) in 48 h, and 1,230.1‍ ‍mL L^-1 of CO₂ and 523.5‍ ‍mL L^-1 of CH₄ were produced. The major isozymes identified by zymograms during the first 12‍ ‍h were 51- and 140-kDa carboxymethyl cellulases (CMCases) and 23- and 57-kDa xylanases. The band densities of 37-, 53-, and 58-kDa CMCases and 38-, 44-, and 130-kDa xylanases increased from 24 to 36 h. A microbial ana-lysis indicated that the relative abundances of Prevotella, Fibrobacter, and Bacteroidales RF16 bacteria, Neocallimastix and Cyllamyces fungi, and Dasytricha and Polyplastron protozoa were related to fibrolytic isozyme activity. The present results provide novel insights into the relationships between fibrolytic isozymes and rumen microorganisms during lignocellulose biodegradation.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}