Microbes and Environments最新文献_第7页

Membrane Potential-requiring Succinate Dehydrogenase Constitutes the Key to Propionate Oxidation and Is Unique to Syntrophic Propionate-oxidizing Bacteria. 需要膜电位的琥珀酸脱氢酶是丙酸氧化的关键，是合养丙酸氧化细菌所特有的。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME22111

Tomoyuki Kosaka, Yuka Tsushima, Yusuke Shiota, Takayuki Ishiguchi, Kazuo Matsushita, Minenosuke Matsutani, Mamoru Yamada

{"title":"Membrane Potential-requiring Succinate Dehydrogenase Constitutes the Key to Propionate Oxidation and Is Unique to Syntrophic Propionate-oxidizing Bacteria.","authors":"Tomoyuki Kosaka, Yuka Tsushima, Yusuke Shiota, Takayuki Ishiguchi, Kazuo Matsushita, Minenosuke Matsutani, Mamoru Yamada","doi":"10.1264/jsme2.ME22111","DOIUrl":"https://doi.org/10.1264/jsme2.ME22111","url":null,"abstract":"Propionate oxidation in Pelotomaculum thermopropionicum is performed under a thermodynamic limit. The most energetically unfavorable reaction in the propionate oxidation pathway is succinate oxidation. Based on previous genomic and transcriptomic ana-lyses, succinate oxidation in P. thermopropionicum under propionate-oxidizing conditions is conducted by the membrane-bound forms of two succinate dehydrogenases (SDHs). We herein examined the activity of SDH, the mechanisms underlying the succinate oxidation reaction in P. thermopropionicum, and the importance of the protein sequences of related genes. SDH activity was highly localized to the membrane fraction. An ana-lysis of the soluble fraction revealed that fumarate reductase received electrons from NADH, suggesting the involvement of membrane-bound SDH in propionate oxidation. We utilized an uncoupler and inhibitors of adenosine triphosphate (ATP) synthase and membrane-bound SDH to investigate whether the membrane potential of P. thermopropionicum supports propionate oxidation alongside hydrogen production. These chemicals inhibited hydrogen production, indicating that membrane-bound SDH requires a membrane potential for succinate oxidation, and this membrane potential is maintained by ATP synthase. In addition, the phylogenetic distribution of the flavin adenine dinucleotide-binding subunit and conserved amino acid sequences of the cytochrome b subunit of SDHs in propionate-oxidizing bacteria suggests that membrane-bound SDHs possess specific conserved amino acid residues that are strongly associated with efficient succinate oxidation in syntrophic propionate-oxidizing bacteria.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9731343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Phosphate-solubilizing Bacteria on Soil Phosphorus Fractions and Supply to Maize Seedlings Grown in Lateritic Red Earths and Cinnamon Soils. 增磷菌对红壤、红壤玉米幼苗土壤磷组分及供给的影响

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME22075

Han Long, Jun Wasaki

{"title":"Effects of Phosphate-solubilizing Bacteria on Soil Phosphorus Fractions and Supply to Maize Seedlings Grown in Lateritic Red Earths and Cinnamon Soils.","authors":"Han Long, Jun Wasaki","doi":"10.1264/jsme2.ME22075","DOIUrl":"https://doi.org/10.1264/jsme2.ME22075","url":null,"abstract":"Phosphorus (P) is often the limiting factor for plant growth because of its low mobility and availability in soils. Phosphate-solubilizing bacteria (PSB) have been shown to increase the availability of soil P fractions, thereby promoting plant growth. We herein investigated the effects of PSB on P availability in two important Chinese soil types: Lateritic red earths (La) and Cinnamon soils (Ci). We initially isolated 5 PSB strains and assessed their effects on soil P fractions. PSB mainly increased moderately labile P in La and labile P in Ci. We then selected the most promising PSB isolate (99% similarity with Enterobacter chuandaensis) and examined its effects on P accumulation in maize seedlings. The results obtained showed that plant P accumulation increased in response to a PSB inoculation in both soil types and the combination of the PSB inoculation and tricalcium phosphate fertilization in La significantly enhanced P accumulation in plant shoots. The present study demonstrated that the PSB isolates tested differed in their ability to mobilize P from distinct P fertilizers and that PSB isolates have potential as a valuable means of sustainably enhancing seedling growth in Chinese agricultural soils.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10104682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Slight Changes in the Gut Microbiome in Early-stage Chronic Kidney Disease of Unknown Etiology. 不明病因慢性肾脏病早期肠道微生物组的轻微变化。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME22097

Ditsayathan Banjong, Thatsanapong Pongking, Na T D Tran, Somchai Pinlaor, Rungtiwa Dangtakot, Kitti Intuyod, Sirirat Anutrakulchai, Ubon Cha'on, Porntip Pinlaor

{"title":"Slight Changes in the Gut Microbiome in Early-stage Chronic Kidney Disease of Unknown Etiology.","authors":"Ditsayathan Banjong, Thatsanapong Pongking, Na T D Tran, Somchai Pinlaor, Rungtiwa Dangtakot, Kitti Intuyod, Sirirat Anutrakulchai, Ubon Cha'on, Porntip Pinlaor","doi":"10.1264/jsme2.ME22097","DOIUrl":"10.1264/jsme2.ME22097","url":null,"abstract":"Gut dysbiosis and changes in short-chain fatty acids (SCFAs) occur in end-stage chronic kidney disease (CKD); however, the degree of these changes in the gut microbiome and serum SCFA profiles in the early stages of CKD,‍ ‍particularly in‍ ‍CKD‍ ‍of unknown etiology (CKDu), is unclear. We herein investigated the gut microbiome and SCFA profiles of early-stage CKD patients (CKD stages 1-3) in a community in Khon Kaen Province, Thailand. Seventy-two parasite-free participants were distributed among a healthy control group (HC, n=18) and three patient groups (an underlying disease group [UD, n=18], early-stage CKD with underlying disease [CKD-UD, n=18], and early-stage CKD of unknown etiology, [CKDu, n=18]). Fecal DNA was individually extracted and pooled for groups of six individuals (three pools in each group) to examine the composition of the gut microbiome using next-generation sequencing. A SCFA ana-lysis was performed on serum samples from each individual using gas chromatography-mass spectrometry. The results revealed that microbial abundance differed between the healthy group and all patient groups (UD, CKD-UD, and CKDu). [Eubacterium]_coprostanoligenes_group was more abundant in the CKDu group than in the HC and CKD-UD groups. Furthermore, serum concentrations of acetate, a major SCFA component, were significantly lower in all patient groups than in the HC group. The present results indicate that minor changes in the gut microbiome and a significant decrease in serum acetate concentrations occur in early-stage CKDu, which may be important for the development of prevention strategies for CKD patients.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10105369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species-specific Microorganisms in Acid-tolerant Chironomus Larvae Reared in a Neutral pH Range under Laboratory Conditions: Single Dataset Analysis. 实验室条件下在中性pH范围内饲养的耐酸摇蚊幼虫中的物种特异性微生物：单数据集分析。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME23029

Sotaro Fujii, Koichiro Kawai, Yoshihiro Sambongi, Satoshi Wakai

{"title":"Species-specific Microorganisms in Acid-tolerant Chironomus Larvae Reared in a Neutral pH Range under Laboratory Conditions: Single Dataset Analysis.","authors":"Sotaro Fujii, Koichiro Kawai, Yoshihiro Sambongi, Satoshi Wakai","doi":"10.1264/jsme2.ME23029","DOIUrl":"10.1264/jsme2.ME23029","url":null,"abstract":"To obtain a more detailed understanding of organismal acid tolerance, the larval microbiomes of 11 Chironomus species collected from acidic or neutral pH areas in Japan and reared at pH 7-8 under laboratory conditions were systematically compared using an amplicon sequencing ana-lysis. Evenness values were lower for the larval microbiomes of acid-tolerant Chironomus cf. riparius, Chironomus fusciceps, and Chironomus sulfurosus than for eight acid-sensitive species based on an alpha diversity ana-lysis. The lower evenness observed suggested a biased abundance of microorganisms, which was consistent with the identification of Chironomus species-specific microorganisms (such as Agromyces mediolanus and Comamonas odontotermitis related bacteria) with high abundance in acid-tolerant larvae. The abundance of specific microorganisms was also high in the microbiome of acid-tolerant larvae of Chironomus acerbiphilus reared at pH 4, but not in that of acid-sensitive larvae. Based on a PICRUSt2 ana-lysis, genes involved in saccharide transport were less abundant in the microbiome of acid-tolerant larvae than in that of acid-sensitive larvae, indicating nutrient-poor acidic environments. Although these results were obtained from single datasets, acid-tolerant larvae appeared to establish Chironomus species-specific interactions with microorganisms independent of saccharides, in contrast to acid-sensitive larvae. The present study is the first step towards understanding organismal acid tolerance.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10728629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71522052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NAD⁺ Synthetase is Required for Free-living and Symbiotic Nitrogen Fixation in the Actinobacterium Frankia casuarinae. 法兰克木麻黄放线菌自由生活和共生固氮需要NAD+合成酶。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME22093

Ken-Ichi Kucho, Koya Asukai, Thanh Van Nguyen

{"title":"NAD+ Synthetase is Required for Free-living and Symbiotic Nitrogen Fixation in the Actinobacterium Frankia casuarinae.","authors":"Ken-Ichi Kucho, Koya Asukai, Thanh Van Nguyen","doi":"10.1264/jsme2.ME22093","DOIUrl":"https://doi.org/10.1264/jsme2.ME22093","url":null,"abstract":"Frankia spp. are multicellular actinobacteria that fix atmospheric dinitrogen (N2) not only in the free-living state, but also in root-nodule symbioses with more than 200 plant species, called actinorhizal plants. To identify novel Frankia genes involved in N2 fixation, we previously isolated mutants of Frankia casuarinae that cannot fix N2. One of these genes, mutant N3H4, did not induce nodulation when inoculated into the host plant Casuarina glauca. Cell lineages that regained the ability to fix N2 as free-living cells were isolated from the mutant cell population. These restored strains also regained the ability to stimulate nodulation. A comparative ana-lysis of the genomes of mutant N3H4 and restored strains revealed that the mutant carried a mutation (Thr584Ile) in the glutamine-dependent NAD+ synthetase gene (Francci3_3146), while restored strains carried an additional suppressor mutation (Asp478Asn) in the same gene. Under nitrogen-depleted conditions, the concentration of NAD(H) was markedly lower in the mutant strain than in the wild type, whereas it was higher in restored strains. These results indicate that glutamine-dependent NAD+ synthetase plays critical roles in both free-living and symbiotic N2 fixation in Frankia.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9225513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Particle-attached Microbes in Eelgrass Vegetation Areas Differ in Community Structure Depending on the Distance from the Eelgrass Bed. 海草植被区附着颗粒的微生物群落结构因与海草床的距离不同而不同。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME23013

Md Mehedi Iqbal, Masahiko Nishimura, Masayoshi Sano, Susumu Yoshizawa

{"title":"Particle-attached Microbes in Eelgrass Vegetation Areas Differ in Community Structure Depending on the Distance from the Eelgrass Bed.","authors":"Md Mehedi Iqbal, Masahiko Nishimura, Masayoshi Sano, Susumu Yoshizawa","doi":"10.1264/jsme2.ME23013","DOIUrl":"10.1264/jsme2.ME23013","url":null,"abstract":"Zostera marina (eelgrass) is a submerged flowering plant often found in the coastal areas of Japan. Large amounts of suspended particles form in highly productive environments, such as eelgrass beds, and the behavior of these particles is expected to affect the surrounding microbial community. We investigated the microbial community structure of suspended particles in three eelgrass fields (Ikuno-Shima Is., Mutsu Bay, and Nanao Bay) and inferred the formation and dynamics of suspended particles from a microbial community structure ana-lysis. Seawater samples were collected directly above each eelgrass bed (eelgrass-covering) and from locations dozens of meters away from the eelgrass bed (bare-ground). In consideration of the two different lifestyles of marine microbes, microbial communities were obtained from particle-attached (PA) and free-living (FL) states. Differences in microbial diversity and community structures were observed between PA and FL in all eelgrass beds. The FL microbial community was similar between the two sampling points (eelgrass-covering and bare-ground), whereas a significant difference was noted in the microbial community structure of suspended particles between the two sampling points. This difference appeared to be due to the supply of organic matter from the eelgrass sea ground and leaf-attached detritus produced by microbial activity. In addition, the classes Flavobacteriia, Alphaproteobacteria, and Gammaproteobacteria were abundant in the PA and FL fractions. Furthermore, many sequences of the key groups (e.g., Planctomycetes and Verrucomicrobia) were exclusively detected in the PA fraction, in which they may circulate nutrients. The present results provide insights into the microbial communities of suspended particles and provide the first step towards understanding their biogeochemical impact on the eelgrass bed.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10207651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Community Structure and Predicted Functions of Actively Growing Bacteria Responsive to Released Coral Mucus in Surrounding Seawater. 对周围海水中释放的珊瑚粘液有反应的活性生长细菌的群落结构和预测功能。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME23024

Akito Taniguchi, Yuki Kuroyanagi, Ryuichiro Aoki, Mitsuru Eguchi

{"title":"Community Structure and Predicted Functions of Actively Growing Bacteria Responsive to Released Coral Mucus in Surrounding Seawater.","authors":"Akito Taniguchi, Yuki Kuroyanagi, Ryuichiro Aoki, Mitsuru Eguchi","doi":"10.1264/jsme2.ME23024","DOIUrl":"10.1264/jsme2.ME23024","url":null,"abstract":"A direct relationship exists between diverse corals and fish farming in Keten Bay, Amami-Oshima, Japan. The release of coral mucus has a significant impact on the microbial activity of surrounding seawater. To obtain a more detailed understanding of biogeochemical cycles in this environment, the effects of coral mucus on the community structure and function of bacteria in surrounding seawater need to be elucidated. We herein used a bromodeoxyuridine approach to investigate the structures and functions of bacterial communities growing close to mucus derived from two different Acropora corals, AC1 and AC2. The alpha diversities of actively growing bacteria (AGB) were lower in mucus-containing seawater than in control seawater and their community structures significantly differed, suggesting that the growth of specific bacteria was modulated by coral mucus. Rhodobacteraceae and Cryomorphaceae species were the most dominant AGB in response to the mucus of Acropora AC1 and AC2, respectively. In contrast, the growth of Actinomarinaceae, Alteromonadaceae, Flavobacteriaceae, and SAR86 clade bacteria was inhibited by coral mucus. The results of a Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) ana-lysis suggested that the predicted functions of AGB in mucus-containing seawater differed from those in seawater. These functions were related to the biosynthesis and degradation of the constituents of coral mucus, such as polysaccharides, sugar acids, and aromatic compounds. The present study demonstrated that complex bacterial community structures and functions may be shaped by coral mucus, suggesting that corals foster diverse bacterial communities that enhance the ecological resilience of this fish farming area.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan. 日本稻田土壤冷适应厌氧细菌的系统发育及生理多样性。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME22109

Sachi Honma, Atsuko Ueki, Akio Ichimura, Kouki Suzuki, Nobuo Kaku, Katsuji Ueki

{"title":"Phylogeny and Physiological Diversity of Cold-adapted Anaerobic Bacteria Isolated from Rice Field Soil in Japan.","authors":"Sachi Honma, Atsuko Ueki, Akio Ichimura, Kouki Suzuki, Nobuo Kaku, Katsuji Ueki","doi":"10.1264/jsme2.ME22109","DOIUrl":"https://doi.org/10.1264/jsme2.ME22109","url":null,"abstract":"Cold-adapted or psychrotrophic fermentative anaerobic bacteria were isolated from rice field soil in a temperate area in Japan using anaerobic enrichment cultures incubated at 5°C. Most isolates were obligately anaerobic, spore-forming rods and affiliated with different lineages of the genus Clostridium based on 16S rRNA gene sequences. The growth temperature ranges and physiological properties of three representative clostridial isolates (C5S7, C5S11T, and C5S18) were examined. Strain C5S7 grew at 0°C, but not at 20°C, and was identified as Clostridium estertheticum, a psychrophile isolated from spoiled, vacuum-packed, chilled meat (blown pack spoilage, BPS). Strain C5S7 produced butyrate, n-butanol, and abundant gases (H2 and CO2) as major fermentation products from the carbohydrates utilized. Strain C5S11T, which was recently described as Clostridium gelidum sp. nov., possessed psychrotrophic properties and grew at temperatures between 0 and 25°C. Strain C5S11T was saccharolytic, decomposed polysaccharides, such as inulin, pectin, and xylan, and produced acetate, butyrate, and gases. Strain C5S18 also grew at 0°C and the optimum growth temperature was 15°C. Strain C5S18 did not ferment carbohydrates and grew in a manner that was dependent on proteinaceous substrates. This strain was identified as the psychrotolerant species, Clostridium tagluense, originally isolated from a permafrost sample. Collectively, the present results indicate that psychrotrophic anaerobic bacteria with different physiological properties actively degrade organic matter in rice field soil, even in midwinter, in a cooperative manner using different substrates. Furthermore, different psychrotrophic species of the genus Clostridium with the ability to cause BPS inhabit cultivated soil in Japan.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10087242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

16S rRNA Gene Sequencing of Six Psyllid Species of the Family Carsidaridae Identified Various Bacteria Including Symbiopectobacterium. Carsidridae科六种木虱的16S rRNA基因测序鉴定了包括共生杆菌在内的多种细菌。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME23045

Junnosuke Maruyama, Hiromitsu Inoue, Yuu Hirose, Atsushi Nakabachi

{"title":"16S rRNA Gene Sequencing of Six Psyllid Species of the Family Carsidaridae Identified Various Bacteria Including Symbiopectobacterium.","authors":"Junnosuke Maruyama, Hiromitsu Inoue, Yuu Hirose, Atsushi Nakabachi","doi":"10.1264/jsme2.ME23045","DOIUrl":"10.1264/jsme2.ME23045","url":null,"abstract":"Psyllids (Hemiptera: Sternorrhyncha: Psylloidea) are plant sap-sucking insects that are closely associated with various microbes. To obtain a more detailed understanding of the ecological and evolutionary behaviors of microbes in Psylloidea, the bacterial populations of six psyllid species, belonging to the family Carsidaridae, were analyzed using high-throughput amplicon sequencing of the 16S rRNA gene. The majority of the secondary symbionts identified in the present study were gammaproteobacteria, particularly those of the order Enterobacterales, including Arsenophonus and Sodalis, which are lineages found in a wide variety of insect hosts. Additionally, Symbiopectobacterium, another Enterobacterales lineage, which has recently been recognized and increasingly shown to be vertically transmitted and mutualistic in various invertebrates, was identified for the first time in Psylloidea. This lineage is closely related to Pectobacterium spp., which are plant pathogens, but forms a distinct clade exhibiting no pathogenicity to plants. Non-Enterobacterales gammaproteobacteria found in the present study were Acinetobacter, Pseudomonas (both Pseudomonadales), Delftia, Comamonas (both Burkholderiales), and Xanthomonas (Xanthomonadales), a putative plant pathogen. Regarding alphaproteobacteria, three Wolbachia (Rickettsiales) lineages belonging to supergroup B, the major group in insect lineages, were detected in four psyllid species. In addition, a Wolbachia lineage of supergroup O, a minor group recently found for the first time in Psylloidea, was detected in one psyllid species. These results suggest the pervasive transfer of bacterial symbionts among animals and plants, providing deeper insights into the evolution of the interactions among these organisms.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10421217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiota in Umbilical Dirt and Its Relationship with Odor. 脐垢中的微生物群及其与气味的关系。

IF 2.2 4区环境科学与生态学

Microbes and Environments Pub Date : 2023-01-01 DOI: 10.1264/jsme2.ME23007

Takehisa Yano, Takao Okajima, Shigeki Tsuchiya, Hisashi Tsujimura

{"title":"Microbiota in Umbilical Dirt and Its Relationship with Odor.","authors":"Takehisa Yano, Takao Okajima, Shigeki Tsuchiya, Hisashi Tsujimura","doi":"10.1264/jsme2.ME23007","DOIUrl":"10.1264/jsme2.ME23007","url":null,"abstract":"The umbilicus accumulates more dirt than other body surfaces and is difficult to clean. Hygiene in this area is vital, particularly for surgery, because of its proximity to the laparotomy site. Although microorganisms in the umbilicus have been extensively examined, those in umbilical dirt have not due to the lack of an efficient method of collection. We previously established a technique to extract umbilical dirt using the anchor effect of polymers, which are injected into the umbilicus. In the present study, we applied this technique for the first time to investigate umbilical dirt. The results obtained revealed an abundance of Corynebacterium among various bacteria, whereas Cutibacterium and Staphylococcus, which are abundant at other skin sites, were rare. The relationships between the microbiota and issues related to the umbilicus were investigated and some covariates, including the odor score and several bacteria, were identified. A detailed ana-lysis of the genera associated with odor revealed no correlation with Corynebacterium; however, some minor anaerobic bacteria, such as Mobiluncus, Arcanobacterium, and Peptoniphilus, were more abundant in the high odor score group. Therefore, this technique to collect umbilical dirt provided insights into the microbiota in umbilical dirt and suggested functions for minor anaerobes. Furthermore, since various pathogenic microorganisms were detected, their control may contribute to the prevention of both odor production and infectious diseases caused by these microorganisms.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10522843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9806723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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