MethodsX最新文献_第5页

Optimizing blue poo: A validated, cost-effective method for measuring whole gut transit time 优化蓝色粪便：一种有效的，具有成本效益的方法来测量整个肠道运输时间

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2025-11-26 DOI: 10.1016/j.mex.2025.103741

Cyra Schmandt , Julia Trunz , Claudio Perret , Anneke Hertig-Godeschalk , Zeno Stanga , Jivko Stoyanov

{"title":"Optimizing blue poo: A validated, cost-effective method for measuring whole gut transit time","authors":"Cyra Schmandt , Julia Trunz , Claudio Perret , Anneke Hertig-Godeschalk , Zeno Stanga , Jivko Stoyanov","doi":"10.1016/j.mex.2025.103741","DOIUrl":"10.1016/j.mex.2025.103741","url":null,"abstract":"<div><div>Whole gut transit time (WGTT) provides essential insights into gastrointestinal health, but traditional measurement methods are often expensive or invasive. This study optimizes and validates the \"blue dye method,\" an affordable and minimally invasive approach to WGTT measurement. Using \"Hollinger Farbpulver Blau\" (containing food colors E131 and E132), dye concentrations ranging from 30 mg to 241 mg were tested across four modes of delivery: capsule with liquid, gummy bear, muffin, and capsule with rice crackers and liquid. Each presented limitations: capsules taken with liquid led to inconsistent transit times, gummy bears caused staining, and muffins were perishable. Measured WGTTs varied between 18 and 29 h depending on the mode of delivery and dye concentration. Optimal protocol was a capsule containing 60 mg of dye taken with two rice crackers and liquid, ensuring accurate detection without practical inconveniences. The standardized and optimized blue dye method provides valid WGTT measurements, making it well suited for large-scale population studies and clinical applications.</div><div>Uses a simple blue dye as a marker for gut transit.</div><div>Tested several modes of delivery and concentrations to find the most practical option.</div><div>Established a standardized protocol for reliable and reproducible measurement.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103741"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145692906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic review of gabion-faced geogrid and pile systems for slope and embankment stability 格宾网面土工格栅和桩系统对边坡和路堤稳定性的系统评价

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2025-12-17 DOI: 10.1016/j.mex.2025.103767

Devi Oktaviana Latif, Virananda Samudera Rahmadhian, Amalia Ula Hazhiyah

{"title":"Systematic review of gabion-faced geogrid and pile systems for slope and embankment stability","authors":"Devi Oktaviana Latif, Virananda Samudera Rahmadhian, Amalia Ula Hazhiyah","doi":"10.1016/j.mex.2025.103767","DOIUrl":"10.1016/j.mex.2025.103767","url":null,"abstract":"<div><div>Slope instability is a major geotechnical hazard intensified by rainfall infiltration, seismic loading, groundwater fluctuations, and human disturbances. Composite reinforcement systems—such as gabion-faced geogrid walls combined with piles or geosynthetic-encased columns (GECs)—are increasingly implemented to address multi-hazard conditions. This study presents a PRISMA-guided systematic review of empirical, numerical, centrifuge, and field investigations on hybrid slope-stabilization systems. The review advances prior work by explicitly incorporating multi-field coupling and soil–structure interaction (SSI) terms into the search strategy, applying transparent screening and data-extraction procedures supported by a reusable metadata codebook, and conducting cross-study triangulation across field evidence, centrifuge modelling, and 2D/3D numerical analyses. The synthesized evidence shows that hybrid systems can significantly enhance slope performance, with reported improvements of up to ∼45 % in factor of safety and >30 % reduction in settlement, depending on reinforcement configuration, soil conditions, and coupled rainfall–seismic effects. The study further highlights current limitations in optimisation practice, long-term monitoring, and design standardisation, and outlines directions for uncertainty-aware and performance-based slope design.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103767"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ex-situ growth protocol for the invasive macrophyte Pontederia crassipes 入侵植物庞德马的迁地生长方案

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2026-01-16 DOI: 10.1016/j.mex.2026.103800

Letícia da Silva Brito , Sidinei Magela Thomaz , Heliana Teixeira , Ana I. Lillebø

{"title":"Ex-situ growth protocol for the invasive macrophyte Pontederia crassipes","authors":"Letícia da Silva Brito , Sidinei Magela Thomaz , Heliana Teixeira , Ana I. Lillebø","doi":"10.1016/j.mex.2026.103800","DOIUrl":"10.1016/j.mex.2026.103800","url":null,"abstract":"<div><div><em>Pontederia crassipes</em> is known for its asexual reproduction and rapid growth. Outside its native range, it has been identified as an environmental threat, while it has also been widely used for <em>ex-situ</em> phytoremediation. To understand both its invasive potential and its phytoremediation capacity, it is necessary to examine the environmental factors that favor its growth beyond those already described in the literature, such as water temperature and nutrient availability. Previous studies also suggest that alkalinity, conductivity, dissolved oxygen, salinity, water depth and pH. These variables help define the species niche and highlight the importance of distinguishing between its fundamental niche, the full set of abiotic conditions that support growth and its realized niche, which reflects biotic interactions and local constraints. However, the scientific literature does not yet provide sufficient description of the <em>ex-situ</em> experimental conditions required for the successful cultivation of this aquatic plant in controlled settings. This protocol therefore reports the results and lessons learned from a series of mesocosm experiments. By standardizing procedures and documenting growth outcomes, the protocol enhances reproducibility, facilitates comparisons across studies and supports both basic and applied research on <em>P. crassipes.</em></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103800"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The neurocultural remake reflex model: An interdisciplinary tool for understanding film remakes 神经文化重拍反射模型：理解电影重拍的跨学科工具

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2026-01-08 DOI: 10.1016/j.mex.2026.103786

Marziya Begum, Akaitab Mukherjee

{"title":"The neurocultural remake reflex model: An interdisciplinary tool for understanding film remakes","authors":"Marziya Begum, Akaitab Mukherjee","doi":"10.1016/j.mex.2026.103786","DOIUrl":"10.1016/j.mex.2026.103786","url":null,"abstract":"<div><div>The <strong>Neurocultural Remake Reflex Model (NRRM)</strong> is an interdisciplinary qualitative and neuroscience-informed analytical method that integrates heuristic insights from intersubject correlation (ISC) literature, cultural memory theory and affect theory to make reception-centered, testable inferences about how remakes trigger recognition, emotion and reinterpretation. The six-steps involved in the framework, <strong>Attention Mapping, Cognitive Processing, Cultural Recall, Cultural Reframing, Emotional Resonance and Emotional Dissonance</strong>are operationalized through systematic qualitative scene analysis rather than empirical neuroimaging<strong>.</strong> Most existing research on remakes has primarily focused on comparative textual analysis, often overlooking the neurocognitive and emotional aspects of audience reception. The NRRM addresses this gap by explaining how viewers attend to, interpret, remember, emotionally connect with, and sometimes resist or contest a remake’s narrative and aesthetic cues. The paper demonstrates the method through a scene-level application of two case studies to show how remakes function simultaneously as mnemonic devices and affective tools. In doing so, NRRM reveals how remakes preserve cultural legacies while generating new meanings, offering film scholars, media psychologists and cultural analysts a reproducible framework for tracing how remakes conserve, reframe or disrupt cultural memory and affective experience.</div><div>This study proposes a six-step systematic qualitative methodology for analyzing remakes.</div><div>By integrating cognitive and cultural approaches, it introduces an interdisciplinary framework for remake analysis.</div><div>This framework demonstrates how remakes function as neurocultural reflexes, triggering nostalgia through both resonance and disruption.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103786"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BioMedStatX – Statistical workflows for reliable biomedical data analysis 用于可靠生物医学数据分析的统计工作流程

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2025-12-17 DOI: 10.1016/j.mex.2025.103776

Philipp Krumm , Nicole Böttcher , Richard Ottermanns , Thomas Pufe , Athanassios Fragoulis

{"title":"BioMedStatX – Statistical workflows for reliable biomedical data analysis","authors":"Philipp Krumm , Nicole Böttcher , Richard Ottermanns , Thomas Pufe , Athanassios Fragoulis","doi":"10.1016/j.mex.2025.103776","DOIUrl":"10.1016/j.mex.2025.103776","url":null,"abstract":"<div><div>Robust statistical analysis is essential for scientific validity and to ensure good scientific practice. Yet many researchers, especially in biomedical fields, struggle with checking assumptions, selecting the correct tests, and interpreting results. These obstacles can lead to misleading conclusions and undermine scientific progress.</div><div>BioMedStatX explicitly addresses these issues by ensuring that the implemented workflows exclude the use of inadequate statistical tests. This Python-based desktop application features an intuitive graphical interface that automatically selects appropriate statistical tests based on the data and its characteristics, ensuring that users, even with minor statistical training, follow a statistically valid workflow.</div><div>Users can import Excel or CSV files, select groups and let BioMedStatX manage the rest: from outlier detection, assumption checks and guided data transformations to test execution (parametric or non-parametric) and guided post-hoc analyses. Results are exported in a structured Excel workbook including a decision tree that visualizes each analytical step, and customizable plots are exported as SVG-/PNG-files.</div><div>By embedding statistical expertise directly into the software, BioMedStatX prevents invalid analysis paths, increases transparency, and enables reproducibility.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103776"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the implementation of maximum entropy sampling with unequal probabilities and without replacement 不等概率无替换的最大熵抽样的实现

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2025-12-30 DOI: 10.1016/j.mex.2025.103780

Philippe Aubry

{"title":"On the implementation of maximum entropy sampling with unequal probabilities and without replacement","authors":"Philippe Aubry","doi":"10.1016/j.mex.2025.103780","DOIUrl":"10.1016/j.mex.2025.103780","url":null,"abstract":"<div><div>Sampling with maximum entropy offers robustness to statistical inference based on randomization theory. However, there were no comprehensive, practical guides explaining how to implement maximum entropy sampling for finite populations with unequal probabilities and without replacement. This article serves as both a toolkit and a reference guide for researchers and engineers, filling a gap in the literature. It links key formal results with ready-to-use algorithms that can be implemented in any procedural programming language. Maximum entropy sampling is straightforward when the sample size is allowed to vary. This is achieved via the <em>Poisson sampling</em> design, in which the sample size is a random variable distributed according to a Poisson binomial distribution. In contrast, the <em>conditional Poisson sampling</em> design, which is obtained by conditioning Poisson sampling on a fixed sample size, has long posed a significant challenge to statisticians.<ul><li><span>•</span><span><div>A compendium of formal results for Poisson sampling, the Poisson binomial distribution, and conditional Poisson sampling is presented.</div></span></li><li><span>•</span><span><div>The computation of inclusion probabilities up to the second order is detailed for the conditional Poisson sampling, and the corresponding algorithms are provided.</div></span></li><li><span>•</span><span><div>Ready-to-use algorithms are provided for implementing Poisson sampling and the Poisson binomial distribution. For conditional Poisson sampling, the rejective, draw-by-draw, sequential, and exchange sampling algorithms are detailed.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103780"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of partial least square structural equation modeling scheme of principal component analysis with importance performance analysis 主成分分析的偏最小二乘结构方程建模方案与重要性能分析的结合

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2025-12-30 DOI: 10.1016/j.mex.2025.103783

Bambang Widjanarko Otok, Zulfani Alfasanah, Diaz Fitra Aksioma

引用次数: 0

Managing missing MUKEYs in the QSWAT+ SSURGO database 管理QSWAT+ SSURGO数据库中缺失的mukey

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2025-12-13 DOI: 10.1016/j.mex.2025.103764

Mahesh R. Tapas , Asmita Murumkar , Chris George , Brian Brandt , Jay Martin

{"title":"Managing missing MUKEYs in the QSWAT+ SSURGO database","authors":"Mahesh R. Tapas , Asmita Murumkar , Chris George , Brian Brandt , Jay Martin","doi":"10.1016/j.mex.2025.103764","DOIUrl":"10.1016/j.mex.2025.103764","url":null,"abstract":"<div><div>The Soil and Water Assessment Tool Plus (SWAT+) is a widely used hydrological model for simulating water flow, sediment transport, and the impacts of land management on watersheds. It relies on soil data to represent how soil properties influence water movement, nutrient cycling, and crop growth within a watershed. The Soil Survey Geographic (SSURGO) database provides detailed, high-resolution soil information essential for such modeling. However, a common challenge arises when missing (Map Unit Keys) in the SWAT+ SSURGO database prevents the creation of Hydrological Response Units (HRUs), effectively halting model development. This study presents a method using QGIS to resolve missing MUKEY issues. Specifically, we demonstrate the use of QGIS's Eliminate Selected Polygons tool with the \"Largest Common Boundary\" option to merge missing MUKEY polygons, maintaining spatial coherence and enabling HRU generation. This approach simplifies the workflow, reduces manual effort, and improves model readiness and accuracy.<ul><li><span>•</span><span><div>The study presents a method for resolving missing MUKEYs in the QSWAT+ SSURGO database, facilitating further development of SWAT+ and minimizing manual errors.</div></span></li><li><span>•</span><span><div>QSWAT+ version 3.0.2 improves error reporting by listing missing MUKEYs in the QGIS log, simplifying troubleshooting.</div></span></li><li><span>•</span><span><div>Using QGIS’s Eliminate Selected Polygons tool with the \"Largest Common Boundary\" option preserves spatial integrity while addressing missing data gaps.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103764"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EMI reduction method of CISPR 36 pre-compliance testing using affordable rubber-based materials CISPR 36预合规测试的EMI减少方法，使用价格合理的橡胶基材料

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2025-12-10 DOI: 10.1016/j.mex.2025.103760

Arief Rufiyanto , Gamantyo Hendrantoro , Reza Septiawan , Eko Setijadi , Budi Sulistya , Sardjono Trihatmo

{"title":"EMI reduction method of CISPR 36 pre-compliance testing using affordable rubber-based materials","authors":"Arief Rufiyanto , Gamantyo Hendrantoro , Reza Septiawan , Eko Setijadi , Budi Sulistya , Sardjono Trihatmo","doi":"10.1016/j.mex.2025.103760","DOIUrl":"10.1016/j.mex.2025.103760","url":null,"abstract":"<div><div>Electric motor performance is greatly affected by emissions from the automotive drive system (drivetrain), necessitating research to mitigate electromagnetic interference (EMI). This study proposes a set of methods that employs simple and inexpensive rubber-based materials as shielding to reduce EMI in electric vehicle modules and further explores suitable materials to reduce emissions. The effectiveness of three different rubber compositions as shielding EMI, focusing on the frequency ranges regulated in the CISPR 36 standard, is investigated as pre-compliance testing in radial and transversal orientations of the measurement antenna. The study shows that using these methods together, the rubber-based materials under test can reduce EMI emissions by shielding effectiveness (SE) from 37.742 dB to 37.362 dB for single layer and 74.874 dB to 75.479 dB for combination of 2 layers with up to 50 % probability across several frequency ranges, especially the frequencies regulated in the CISPR 36 standard.</div><div>A realistic method to provide a reasonably cost-effective solution to reduce EMI, particularly for electric cars in the pre-compliance stage, using simple and inexpensive materials, mainly rubber-based materials,</div><div>EMI mitigation method using organic material as an absorber for pre-compliance testing in the frequency range of the CISPR 36 standard,</div><div>Method to determine the best combination of materials to reduce the emissions that arise from the electrical module of the DUT.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103760"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative scoping review approach: identifying the intersection of carbon, biodiversity, and water offsetting 一种比较范围审查方法：确定碳、生物多样性和水抵消的交集

IF 1.9

MethodsX Pub Date : 2026-06-01 Epub Date: 2026-01-13 DOI: 10.1016/j.mex.2026.103799

Felice Diekel, Rosalie Arendt, Markus Berger

{"title":"A comparative scoping review approach: identifying the intersection of carbon, biodiversity, and water offsetting","authors":"Felice Diekel, Rosalie Arendt, Markus Berger","doi":"10.1016/j.mex.2026.103799","DOIUrl":"10.1016/j.mex.2026.103799","url":null,"abstract":"<div><div>Environmental and climate policies, as well as the knowledge underpinning them, are often developed in isolation. This is evident in offsetting research and policy, which tend to address carbon, biodiversity, and water as separate issues. This paper presents the development of an adapted scoping review methodology to compare these three distinct bodies of literature within a unified framework, which also allows for the introduction of the emerging water offsetting literature. The approach ensures comparability across datasets of relevant literature while addressing the challenge of managing large volumes of literature within time and resource constraints. It provides a practical solution for managing diverse bodies of literature in scoping reviews, enabling a holistic understanding of the interrelationships among carbon, biodiversity, and water offsetting.</div><div>Key elements of the method include:<ul><li><span>•</span><span><div>Applying a consistent approach across all three datasets, while accommodating the specificities of each.</div></span></li><li><span>•</span><span><div>Utilizing the machine learning tool ASReviewer to streamline the screening process, alongside a pilot screening phase to establish consistent inclusion criteria.</div></span></li><li><span>•</span><span><div>Combining quantitative bibliometric analysis with qualitative thematic analysis.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103799"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0