MethodsX最新文献

{"title":"Developing a new tool to capture the nature dose to reduce loneliness and improve quality of life","authors":"Ashby Lavelle Sachs ,&nbsp;Montse Maso-Aguado ,&nbsp;Albert Bach ,&nbsp;Nerkez Opacin ,&nbsp;Nicholas Hill ,&nbsp;Lucie Cattaneo ,&nbsp;Laura Coll-Planas ,&nbsp;Katherine Johnson ,&nbsp;Laura Hidalgo ,&nbsp;Carolyn Daher ,&nbsp;Jill Litt ,&nbsp;Sarah Bekessy","doi":"10.1016/j.mex.2024.102969","DOIUrl":"10.1016/j.mex.2024.102969","url":null,"abstract":"<div><div>Recognition of the health benefits of nature contact has increased. Simultaneously, growing numbers of people worldwide experience loneliness. There is a movement towards prescribing nature-based activities to improve/promote social connections, health, and quality of life. Yet, what constitutes a therapeutic nature dose is not well understood, due in part, to the lack of instruments that capture the characteristics of nature-based activities and measure ‘nature dose.’ We created a nature dose measurement tool to fill this gap by capturing various aspects of contact with nature and perceptions regarding park access, quality, naturalness, psychological distance to nature, and biodiversity. This tool will facilitate greater understanding of how natural areas, nature-based activities, and nature exposure reduce loneliness and promote health-related quality of life. Measuring nature dose with standardized tools and documenting benefits will generate</div><div>the evidence base needed to design, implement and evaluate nature-based social interventions for improving health and quality of life.<ul><li><span>•</span><span><div>This tool captures the nature dose to reduce loneliness and promote quality of life.</div></span></li><li><span>•</span><span><div>Constructs range from park quality and access, to mood, to biodiversity perceptions.</div></span></li><li><span>•</span><span><div>The standardized nature dose tool will help design nature-based social interventions.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102969"},"PeriodicalIF":1.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124004205/pdfft?md5=aea2a32aba116c5d19435dc3ef5793bb&pid=1-s2.0-S2215016124004205-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Explainable rotation-invariant self-supervised representation learning","authors":"Devansh Singh ,&nbsp;Aboli Marathe ,&nbsp;Sidharth Roy ,&nbsp;Rahee Walambe ,&nbsp;Ketan Kotecha","doi":"10.1016/j.mex.2024.102959","DOIUrl":"10.1016/j.mex.2024.102959","url":null,"abstract":"<div><p>This paper describes a method that can perform robust detection and classification in out-of-distribution rotated images in the medical domain. In real-world medical imaging tools, noise due to the rotation of the body part is frequently observed. This noise reduces the accuracy of AI-based classification and prediction models. Hence, it is important to develop models which are rotation invariant. To that end, the proposed method - RISC (rotation invariant self-supervised vision framework) addresses this issue of rotational corruption. We present state-of-the-art rotation-invariant classification results and provide explainability for the performance in the domain. The evaluation of the proposed method is carried out on real-world adversarial examples in Medical Imagery-OrganAMNIST, RetinaMNIST and PneumoniaMNIST. It is observed that RISC outperforms the rotation-affected benchmark methods by obtaining 22%, 17% and 2% accuracy boost on OrganAMNIST, PneumoniaMNIST and RetinaMNIST rotated baselines respectively. Further, explainability results are demonstrated.</p><p>This methods paper describes:</p><ul><li><span>•</span><span><p>a representation learning approach that can perform robust detection and classification in out-of-distribution rotated images in the medical domain.</p></span></li><li><span>•</span><span><p>It presents a method that incorporates self-supervised rotation invariance for correcting rotational corruptions.</p></span></li><li><span>•</span><span><p>GradCAM-based explainability for the rotational SSL pretext task and the downstream classification outcomes for the three benchmark datasets are presented</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102959"},"PeriodicalIF":1.6,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124004102/pdfft?md5=960ede7733b4b76435ee298fd9a1d9b1&pid=1-s2.0-S2215016124004102-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximizing single cell dissociation protocol for individual zebrafish embryo","authors":"Shruthi VijayKumar,&nbsp;Michael Borja,&nbsp;Norma Neff,&nbsp;Loïc A. Royer,&nbsp;Merlin Lange","doi":"10.1016/j.mex.2024.102958","DOIUrl":"10.1016/j.mex.2024.102958","url":null,"abstract":"<div><p>Single-cell sequencing has revolutionized our understanding of cellular heterogeneity and cell state, enabling investigations across diverse fields such as developmental biology, immunology, and cancer biology. However, obtaining a high-quality single-cell suspension is still challenging, particularly when starting with limited materials like Zebrafish embryos, a powerful animal model for studying developmental processes and human diseases. Here, we present an optimized protocol for isolating single cells from individual zebrafish embryos, offering a valuable resource for researchers interested in working with limited starting material. The protocol facilitates unique investigations utilizing individual embryos, such as inter-individual genetic differences and embryo-specific lineage tracing analysis. Using a refined single-cell isolation protocol alongside zebrafish as a model organism, researchers can access a resource for exploring the emergence of all types and states of cells, advancing our understanding of cellular processes and disease mechanisms.</p></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102958"},"PeriodicalIF":1.6,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124004096/pdfft?md5=7985c5550bbcb1998dc3eb96d4d77256&pid=1-s2.0-S2215016124004096-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Step-by-step synthetic route to access eugenol-1,2,3-triazole-chalcone hybrid","authors":"Atta Ullah ,&nbsp;Bayu Ardiansah ,&nbsp;Antonius Herry Cahyana ,&nbsp;Abad Ali","doi":"10.1016/j.mex.2024.102956","DOIUrl":"10.1016/j.mex.2024.102956","url":null,"abstract":"<div><p>Molecular hybridization represents a strategic approach in drug design, where two or more pharmacophoric elements from distinct bioactive molecules are integrated into a single hybrid compound. In this study, we synthesized hybrid compounds of chalcone, triazole, and eugenol through straightforward reactions using 4-hydroxyacetophenone as the starting material. Initially, 4-hydroxyacetophenone (<strong>1</strong>) underwent alkylation with 1,4-dibromobutane to produce compound <strong>2</strong> with an 84 % yield. Compound <strong>2</strong> was then subjected to azidation, resulting in azidobutoxyacetophenone <strong>3</strong> with a 71 % yield. Subsequently, compound <strong>3</strong> was reacted with either benzaldehyde or 4-methoxybenzaldehyde via base-catalyzed aldol condensation, yielding azidobutoxychalcones <strong>4a</strong> (69 %) and <strong>4b</strong> (84 %). Finally, azide-alkyne [3+2] cycloaddition between <strong>4a/4b</strong> and propargylated eugenol afforded chalcone derivatives bearing eugenol-1,2,3-triazole hybrids 5a and 5b, each with a 90 % yield.</p><ul><li><span>•</span><span><p>Synthesized chalcones featuring an eugenol-1,2,3-triazole scaffold using 4-hydroxyacetophenone as the starting material.</p></span></li><li><span>•</span><span><p>Synthesis was accomplished through a four-step reaction sequence.</p></span></li><li><span>•</span><span><p>Products were obtained in good yield.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102956"},"PeriodicalIF":1.6,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124004072/pdfft?md5=4b5eedfc3f250a812c08b771d37641eb&pid=1-s2.0-S2215016124004072-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GAM4water: An R-based method for extracting wetted areas from remotely-sensed images","authors":"Matteo Redana ,&nbsp;Lesley T. Lancaster ,&nbsp;Chris Gibbins","doi":"10.1016/j.mex.2024.102955","DOIUrl":"10.1016/j.mex.2024.102955","url":null,"abstract":"<div><div>We present ‘GAM4water,’ a R-based method to classify wetted and non-wetted (dry) areas using remotely sensed image indices derived from such images. The GAM4water classification algorithm is built around a Generalized Additive Model (GAM) capable of accounting for non-linear responses. GAM4water can use any type of radiometric data, whether from drones, satellites or other platforms, and can be used with data of different spatial resolutions, geographic extents and spatial reference systems. It is a supervised tool that uses pixel information to distinguish between wetted and dry areas within an image set, extract them and produce a rich output that includes a binary raster, polygons of wetted areas, and a classification performance report. We tested the method in two case-studies, one using high resolution drone images and another using satellite images. The tests show that GAM4water can produce highly accurate classifications of wetted and non-wetted areas, and has the additional benefit of being easily customizable and not requiring complex implementation procedures.<ul><li><span>•</span><span><div>This paper introduces the first R based method of wetted area extraction for remotely-sensed images.</div></span></li><li><span>•</span><span><div>The method is based on Generalized Additive Models and is applicable to any remotely-sensed data.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102955"},"PeriodicalIF":1.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124004060/pdfft?md5=f3b7be30721e0a71e17718184a15d09a&pid=1-s2.0-S2215016124004060-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of growth parameters for the extremely acidophilic archaeon Ferroplasma acidiphilum DSM 12658 using a two-step turbidimetric growth measurement method","authors":"Montri Yasawong ,&nbsp;Thunwarat Songngamsuk","doi":"10.1016/j.mex.2024.102953","DOIUrl":"10.1016/j.mex.2024.102953","url":null,"abstract":"<div><p>The acidophilic archaeon <em>Ferroplasma acidiphilum</em> exhibits remarkable adaptations to life in highly acidic environments. Nevertheless, the investigation of its molecular biology is challenging because of the slow growth of the organism, low biomass yield, and limitations of standard growth measurement techniques caused by iron oxidation.</p><ul><li><span>•</span><span><p>A novel two-step turbidimetric growth measurement (2TGM) method was developed to address the shortcomings associated with iron precipitation in culture media. The method comprises two distinct preparation steps.</p></span></li><li><span>•</span><span><p>The initial 2TGM step involves centrifuging culture samples at high speed to remove <em>F. acidiphilum</em> cells and iron precipitates, thus preparing a blank.</p></span></li><li><span>•</span><span><p>The second 2TGM step involves centrifuging the culture at a lower speed with the objective of selectively removing iron precipitates, thus allowing <em>F. acidiphilum</em> cells to remain suspended in the supernatant. This preparation enables subsequent measurement of cell density.</p></span></li></ul>A study on the growth of <em>F. acidiphilum</em> showed a standard logistic pattern with a 35-h lag phase, approximately 9-h doubling time, and 0.042 OD carrying capacity. A new two-step turbidimetric growth measurement (2TGM) method overcomes limitations of existing approaches, enabling further investigation of <em>F. acidiphilum</em>.</div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102953"},"PeriodicalIF":1.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124004047/pdfft?md5=894524ae4f3e1a65aab174361c237f7c&pid=1-s2.0-S2215016124004047-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142172468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adsorption performance with field emission scanning electron microscopy of fruit peel induced Silver Nanoparticles in C16H18ClN3S for waste water treatment","authors":"Jyolsna P,&nbsp;Gowthami V,&nbsp;Hajeera Aseen A","doi":"10.1016/j.mex.2024.102951","DOIUrl":"10.1016/j.mex.2024.102951","url":null,"abstract":"<div><p>There is a growing demand for cost-effective and sustainable technologies for treating wastewater as water consumption increases and conventional technologies become more expensive. Nanoparticles have a great deal of potential for use in the treatment of waste water. Their unique surface area allows them to effectively remove toxic metal ions, pathogenic microorganisms, organic and inorganic solutes from water. This study investigated the potential of orange and banana peels as renewable nano adsorbents for removing dyes and dissolved organic compounds from textile wastewater. Orange and banana peels are an optimal selection due to their favourable chemical characteristics, namely the presence of cellulose, pectic, hemicellulose, and lignin. Their capacity to adsorb diverse anionic and cationic compounds on their surface-active sites is attributed to their unique functional group compositions. Silver nanoparticles are able to adsorb heavy metals due to their exceptionally low electrical and thermal resistance and surface plasmon resonance. The samples were thoroughly characterised using field emission scanning electron microscopy (FESEM), UV–Visible spectrometry, Fourier transform infrared spectroscopy (FTIR) and XRD. The nanoparticles were prepared (10 gm,50 gm,100 gm) and subsequently introduced to the wastewater sample. The optical density values were recorded at various time points. The optical density values demonstrate a decline over the course of the experiment, with a notable decrease observed over time. The results of this study provide valuable insights into the efficacy of these natural adsorbents and their potential for sustainable water purification technologies. For the purpose of this research, high performance instrumentation methods were performed as follows:</p><ul><li><span>•</span><span><p>Field emission scanning electron microscopy for surface morphology studies.</p></span></li><li><span>•</span><span><p>Gas chromatography-mass spectrometry (GC–MS) for analytical technique that combines gas chromatography (GC) and mass spectrometry (MS) to identify unknown substances or contaminants.</p></span></li><li><span>•</span><span><p>Optical density values were measured for different timings of degradation.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102951"},"PeriodicalIF":1.6,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124004023/pdfft?md5=2108bd0864d8b2b8608f1a74705e20a9&pid=1-s2.0-S2215016124004023-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The study of kinetic of silver catalytic degradation of phoxim","authors":"Gholamreza Shams ,&nbsp;Mohammad Javad Chaichi ,&nbsp;Jalal Hassan ,&nbsp;Ali Pourshaban-Shahrestani","doi":"10.1016/j.mex.2024.102927","DOIUrl":"10.1016/j.mex.2024.102927","url":null,"abstract":"<div><div>In this study, we scrutinized the degradation process of phoxim in the presence of Ag+ ions, maintaining a 1:1 molar ratio under diverse temperature conditions. Phoxim was chosen as the model compound to devise experimental methodologies that would shed light on the kinetic and degradation pathways within a time span of 0 to 184 min across varying temperatures. The Arrhenius equation was harnessed to ascertain the activation energies linked with the degradation of phoxim. The application of the Arrhenius equation enables the computation of the reaction constant at a given temperature, thereby paving the way for the prediction of phoxim concentrations at different temperatures. The second-order rate constant for the reaction was observed to lie within the range of 0.035 to 0.128 L mol-1min-1, and the half-life of the reaction fluctuated between 5.2 and 17 min across different temperatures.<ul><li><span>•</span><span><div>The study investigates the degradation of phoxim in the presence of Ag+ ions at various temperatures.</div></span></li><li><span>•</span><span><div>The Arrhenius equation was used to calculate the activation energies and predict phoxim concentrations at different temperatures.</div></span></li><li><span>•</span><span><div>The second-order rate constant for the reaction ranged from 0.035 to 0.128 L mol-1min-1, with the half-life varying between 5.2 and 17 min.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102927"},"PeriodicalIF":1.6,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003789/pdfft?md5=20105c1baa06c84a365c841676e29f85&pid=1-s2.0-S2215016124003789-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usability testing of a conceptual model through retrospective cross-case analysis","authors":"John Paul Mynott ,&nbsp;Klara Kager","doi":"10.1016/j.mex.2024.102950","DOIUrl":"10.1016/j.mex.2024.102950","url":null,"abstract":"<div><p>Qualitative conceptual models are commonly used in the scientific literature to make complex phenomena easier to understand. However, the effectiveness and usability of conceptual models to serve as analytical tools is rarely explored and tested, and there is a lack of guidelines for such analyses. This paper adapts and combines the methods of usability testing and cross-case analysis to describe a systematic protocol to facilitate the qualitative evaluation of conceptual models. Usability testing is an established method for identifying problems or shortcomings within a product and for assessing different dimensions of product usability: suitability, accessibility, relevance, and integrity. Cross-case analysis, on the other hand, is a qualitative research method for systematically comparing information from individual case studies and identifying commonalities and patterns that apply across cases. Taken together, these methodological approaches provide a structured way of retrospectively applying a conceptual model to existing literature and thereby evaluating its effectiveness in meeting its intended purpose.</p><ul><li><span>•</span><span><p>We show how researchers can prepare, conduct, and synthesise the results of a usability test of a conceptual model.</p></span></li><li><span>•</span><span><p>We provide recommendations for the practical implementation of each step.</p></span></li><li><span>•</span><span><p>We outline the benefits, limitations, and ethical considerations that researchers should be aware of.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102950"},"PeriodicalIF":1.6,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124004011/pdfft?md5=fdba2af9f055ee81101d9d096089646f&pid=1-s2.0-S2215016124004011-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142148880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine mesocosm system: A reliable tool for testing bioaccumulation and effects of seawater enrichment with dissolved iron in reef organisms","authors":"Adalto Bianchini ,&nbsp;Letícia May Fukushima ,&nbsp;Ana Carolina Grillo ,&nbsp;Kelly Yumi Inagaki ,&nbsp;Juliana de Andrade Souza ,&nbsp;Ligia Salgado Bechara ,&nbsp;Giovanna Destri ,&nbsp;Vitor André Passos Picolotto ,&nbsp;Thales Jean Vidal ,&nbsp;Alessandra Lopes de Araújo ,&nbsp;Juliana da Silva Fonseca ,&nbsp;Emiliano Nicolas Calderon ,&nbsp;Kely Paula Salvi ,&nbsp;Carlos Henrique Figueiredo Lacerda ,&nbsp;Patrícia Gomes Costa ,&nbsp;Miguel Mies","doi":"10.1016/j.mex.2024.102949","DOIUrl":"10.1016/j.mex.2024.102949","url":null,"abstract":"<div><p>In 2015, a marine mesocosm facility was designed and implemented by the Coral Vivo Project in its research station (Porto Seguro, Bahia State, Brazil) to initially study the effects of global impacts, especially ocean warming and acidification, on coral reefs. However, local impacts, including seawater contamination with metal(loid)s, are considered as a major threat to coral reefs. Also, in 2015, the largest disaster involving a mining dam occurred in Brazil. Iron (Fe) mining tailings originated from the dam failure affected not only freshwater ecosystems (rivers, lakes and lagoons), but also adjacent beaches, mangroves, restingas, reefs and other marine systems. Seawater, sediments and biota were contaminated with metal(loid)s, especially Fe, arsenic (As), mercury (Hg) and manganese (Mn). Therefore, we aimed to adapt the marine mesocosm facility of the Coral Vivo Project to evaluate the bioaccumulation and biological impacts of increasing concentrations of dissolved Fe on a diversity of reef organisms. Results obtained indicate a great versatility and reliability of the marine mesocosm system for application in biological and ecological studies on the isolated effect of seawater dissolved Fe on reef organisms of different functional groups simultaneously.</p><ul><li><span>•</span><span><p>Studies involving seawater enrichment with dissolved Fe can be performed using a marine mesocosm system.</p></span></li><li><span>•</span><span><p>The marine mesocosm is a reliable tool to study the isolated effects of metal(loid)s on reef organisms.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102949"},"PeriodicalIF":1.6,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S221501612400400X/pdfft?md5=24acf4a96b97e818950123e8a1102f13&pid=1-s2.0-S221501612400400X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142148791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}