MethodsXPub Date : 2024-08-08DOI: 10.1016/j.mex.2024.102894
Anam Raza , Imran Mahmood , Tayyaba Sultana
{"title":"Methods to quantify gait rehabilitation following lower limb fractures","authors":"Anam Raza , Imran Mahmood , Tayyaba Sultana","doi":"10.1016/j.mex.2024.102894","DOIUrl":"10.1016/j.mex.2024.102894","url":null,"abstract":"<div><p>Lower limb fragility fractures require long-term rehabilitation and are also very expensive to treat. Clinically, early weight bearing and walking stability were reported as key measures of fracture restoration. This study introduces methods to numerically quantify these performance indices for a range of ankle and knee joint fractures. As a follow-up of initial treatment, experimental data was collected using force plates from 367 subjects divided into seven groups: ankle fracture (AF), lower leg ankle fracture (AL), calcaneus foot fracture (CF), knee tibia fracture (KF), knee patella fracture (KP), kneecap rupture (KR), and normal limb (NL). For each joint, data was analysed to evaluate intralimb and interlimb weight-bearing and walking stability for all fracture conditions. These thresholds were statistically compared with normal subjects. Some advantages of evaluating fracture restoration indices over the others include:</p><ul><li><span>•</span><span><p>to quantify fracture restoration (weight-bearing, walking stability, and gait symmetry) using minimum setup and signal requirements.</p></span></li><li><span>•</span><span><p>to provide comprehensive tools to assess and overcome fracture-associated complications through a detailed preview of fractured limb functionality during subphases of a gait cycle.</p></span></li><li><span>•</span><span><p>in clinical research, such assessments are important as a reference to evaluate existing or new rehabilitative interventions.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102894"},"PeriodicalIF":1.6,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003467/pdfft?md5=173a41de64e273203b892357ae3cba17&pid=1-s2.0-S2215016124003467-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2024-08-08DOI: 10.1016/j.mex.2024.102889
Matheus C. Carvalho , Paula Gomez-Alvarez , Luke C. Jeffrey , Damien Troy Maher
{"title":"Improving the measurement of nitrogen stable isotopes in organic materials containing high C:N ratios using a 5A molecular sieve column","authors":"Matheus C. Carvalho , Paula Gomez-Alvarez , Luke C. Jeffrey , Damien Troy Maher","doi":"10.1016/j.mex.2024.102889","DOIUrl":"10.1016/j.mex.2024.102889","url":null,"abstract":"<div><p>The nitrogen stable isotope composition (δ<sup>15</sup>N) of plant materials has numerous applications. Plant materials like bark can have a very high C:N ratio. Incomplete C combustion in such samples interferes with the δ<sup>15</sup>N measurement due to CO production. We modified the standard setup for δ<sup>15</sup>N measurement using an elemental analyzer (EA) coupled to an isotope ratio mass spectrometer (IRMS) by incorporating a 5A molecular sieve column, which better separates N<sub>2</sub> from CO. We compared this new modified setup and the standard one for the measurement of bark samples. Precision and accuracy for δ<sup>15</sup>N in standards with low C:N ratio were equivalent for the two methods. However, for bark the results obtained with the new method had better precision and accuracy than the standard method. <strong>Replicates are nevertheless recommended with the new method to ensure confidence in the results.</strong></p><ul><li><span>•</span><span><p>During elemental analysis, incomplete combustion of material with high C:N ratio can lead to CO formation, which interferes with δ<sup>15</sup>N IRMS measurements.</p></span></li><li><span>•</span><span><p>Here we use a 5A molsieve column to remove the CO interference in δ<sup>15</sup>N measurements Precision and accuracy on δ<sup>15</sup>N measurements of samples with high C content are significantly improved</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102889"},"PeriodicalIF":1.6,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003418/pdfft?md5=b26da25765ee88c19104b645709e0b9f&pid=1-s2.0-S2215016124003418-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2024-08-06DOI: 10.1016/j.mex.2024.102884
Daichi Nakajima , Nozomi Takahashi , Takanari Inoue , Shin-ichiro M. Nomura , Hideaki T. Matsubayashi
{"title":"A unified purification method for actin-binding proteins using a TEV-cleavable His-Strep-tag","authors":"Daichi Nakajima , Nozomi Takahashi , Takanari Inoue , Shin-ichiro M. Nomura , Hideaki T. Matsubayashi","doi":"10.1016/j.mex.2024.102884","DOIUrl":"10.1016/j.mex.2024.102884","url":null,"abstract":"<div><p>The actin cytoskeleton governs the dynamic functions of cells, ranging from motility to phagocytosis and cell division. To elucidate the molecular mechanism, <em>in vitro</em> reconstructions of the actin cytoskeleton and its force generation process have played essential roles, highlighting the importance of efficient purification methods for actin-binding proteins. In this study, we introduce a unified purification method for actin-binding proteins, including capping protein (CP), cofilin, ADF, profilin, fascin, and VASP, key regulators in force generation of the actin cytoskeleton. Exploiting a His-Strep-tag combined with a TEV protease cleavage site, we purified these diverse actin-binding proteins through a simple two-column purification process: initial purification through a Strep-Tactin column and subsequent tag removal through the reverse purification by a Ni-NTA column. Biochemical and microscopic assays validated the functionality of the purified proteins, demonstrating the versatility of the approach. Our methods not only delineate critical steps for the efficient preparation of actin-binding proteins but also hold the potential to advance investigations of mutants, isoforms, various source species, and engineered proteins involved in actin cytoskeletal dynamics.</p><ul><li><span>•</span><span><p>Unified purification method for various actin-binding proteins.</p></span></li><li><span>•</span><span><p>His-Strep-tag and TEV protease cleavage for efficient purification.</p></span></li><li><span>•</span><span><p>Functional validation through biochemical and microscopic assays.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102884"},"PeriodicalIF":1.6,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003364/pdfft?md5=297b77b7062c95cd4a0559dd1a6cb502&pid=1-s2.0-S2215016124003364-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141964310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of toxicological relevant arsenic species in urine by hydride generation microwave-induced plasma optical emission spectrometry","authors":"Paulina Pizzorno , Lucía Falchi , Nelly Mañay , Mariela Pistón , Valery Bühl","doi":"10.1016/j.mex.2024.102893","DOIUrl":"10.1016/j.mex.2024.102893","url":null,"abstract":"<div><p>An analytical method for the determination of toxicological relevant species of arsenic in urine was developed and validated using hydride generation microwave-induced emission spectrometry (HG-MP-AES). This strategy can be used as an alternative to HG-HPLC-ICP-MS considered as a reference technique for arsenic speciation. This procedure is notably less expensive than other techniques and sample preparation and requires only a few steps.</p><ul><li><span>•</span><span><p>Hydride generation with MP-AES detection has proven to be an effective technique for measuring arsenic metabolites in urine, which is relevant for occupational monitoring and health risk assessment purposes.</p></span></li><li><span>•</span><span><p>This method offers simplicity and cost-effectiveness, serving as an alternative to classical analytical procedures typically used for arsenic analysis in urine.</p></span></li><li><span>•</span><span><p>The methodology has been successfully applied for the purpose of workers' health surveillance.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102893"},"PeriodicalIF":1.6,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003455/pdfft?md5=f59eef942f17c251157e6eb1ce5c7092&pid=1-s2.0-S2215016124003455-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2024-08-06DOI: 10.1016/j.mex.2024.102899
Insun Song
{"title":"Constant head-transient method using pressure and flow data for determining permeability and specific storage of tight rocks","authors":"Insun Song","doi":"10.1016/j.mex.2024.102899","DOIUrl":"10.1016/j.mex.2024.102899","url":null,"abstract":"<div><p>This paper describes a ‘constant head-transient method’ for estimating permeability and specific storage of tight rock samples, such as shale and crystalline rocks. Experimental tests are conducted using a cylindrical rock sample subjected to confining pressure, through which pressure diffusion occurs from a constant upstream pressure (or constant head) to a finite downstream storage. Unlike the pulse-transient method, the upstream fluid flow into the sample can be measured using a syringe pump because of no change in upstream pressure. By minimizing the downstream storage, the test time can be significantly reduced, but only the downstream pressure transient data do not yield accurate results on permeability and specific storage estimations. By combining the flow data with the pressure data, the proposed method aims at saving the test time and improving the accuracy of their estimations in extremely low permeability rock samples.</p><ul><li><span>•</span><span><p>A constant head-transient method for measuring the hydraulic properties of tight rocks was developed with a boundary condition of constant upstream pressure and finite downstream storage.</p></span></li><li><span>•</span><span><p>The test time can be saved by minimizing the downstream storage, and the upstream flow can be measured to improve the accuracy in measuring the hydraulic properties.</p></span></li><li><span>•</span><span><p>Combining the flow and pressure objective functions yields the best curve fitting for both pressure and flow curves.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102899"},"PeriodicalIF":1.6,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003510/pdfft?md5=abb0ffe6280da0e090c741642769fb6c&pid=1-s2.0-S2215016124003510-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2024-08-06DOI: 10.1016/j.mex.2024.102883
Marli Burger , Andrea B. Webster , Tshepiso L. Majelantle , Juan Scheun , Andre Ganswindt
{"title":"Method development: Assigning sex to African clawless otter spraints and assessing stability of faecal androgen and progestagen metabolites post-defaecation","authors":"Marli Burger , Andrea B. Webster , Tshepiso L. Majelantle , Juan Scheun , Andre Ganswindt","doi":"10.1016/j.mex.2024.102883","DOIUrl":"10.1016/j.mex.2024.102883","url":null,"abstract":"<div><p>Monitoring reproductive physiology in wildlife can be a useful tool for assessing population dynamics for conservation and management purposes. Utilizing non-invasive approaches for this, such as quantifying reproductive hormone metabolites from faeces, can be challenging when defaecation events are not observed, or when cryptic species like African clawless otters (<em>Aonyx capensis</em>) are involved. Additionally, test systems for quantifying hormone metabolites in a species for the first time must first be reliably validated prior to use. Our results indicate that Epiandrosterone and Progesterone EIAs are most suitable for determining fAM and fPM concentrations in African clawless otter spraints. The fAM:fPM ratio and respective thresholds are more reliable in sex identification compared to the separate use of individual hormone classes. Sex-related hormone metabolite concentrations remained comparable for up to 12hrs post-defaecation in both sexes.</p><ul><li><span>•</span><span><p>We screened two androgen and two progestagen enzyme-immunoassays (EIAs) for suitability and reliable quantification of faecal androgen metabolites (fAM) and faecal progestagen metabolites (fPM) in African clawless otters.</p></span></li><li><span>•</span><span><p>We assessed whether the ratio of fAM:fPM concentrations can be used to assign sex to faecal samples from unknown individuals.</p></span></li><li><span>•</span><span><p>We tested the stability of fAM and fPM concentrations post-defaecation to determine the effects of environmental exposure and bacterial metabolism.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102883"},"PeriodicalIF":1.6,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003352/pdfft?md5=6a7d909985dcc4139dfcb4ba13cfc034&pid=1-s2.0-S2215016124003352-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141964336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2024-08-05DOI: 10.1016/j.mex.2024.102890
Marcio Pereira Basilio , Valdecy Pereira , Fatih Yiğit
{"title":"New hybrid EC-PROMETHEE method with multiple iterations of random weight ranges: Step-by-step application in Python","authors":"Marcio Pereira Basilio , Valdecy Pereira , Fatih Yiğit","doi":"10.1016/j.mex.2024.102890","DOIUrl":"10.1016/j.mex.2024.102890","url":null,"abstract":"<div><p>The decision-making process consists of finding the best solution to an analyzed problem. This search is carried out in the face of countless interactions when analyzing an alternative criterion by criterion, under which weights are assigned that distinguish the degree of importance they have for the decision-makers. The definition of weight for each criterion gives rise to three lines of thought on the subject. There are objective, subjective, and hybrid methods. This discussion concerns the degree to which experts define the criteria weights. Based on this discussion, we developed a hybrid method to integrate the Entropy and CRITIC methods with the PROMETHEE method, called EC-PROMETHEE. The innovation of this method is that the combination of the Entropy and CRITIC methods does not result in a single set of weights. In reality, the weights generated by each method are used to define each criterion's upper and lower limits. The range of weights generated for each criterion is emulated \"n\" times and builds a set of weights that are applied to the ranking definition process. The model generates \"n\" rankings, defining a single ranking. In this article, we demonstrate a step-by-step application of a tool developed in Python called EC-PROMETHEE and use it as an example of the problem of choosing rotary-wing airplanes for application in the military police service.</p><ul><li><span>➢</span><span><p><em>The method reduces discretion in determining the weights of the criteria;</em></p></span></li><li><span>➢</span><span><p><em>The innovation lies in the use of a range of weights for criteria;</em></p></span></li><li><span>➢</span><span><p><em>Consistency in defining the final ranking.</em></p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102890"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S221501612400342X/pdfft?md5=5e157af593ecb240b88ee271334cb59f&pid=1-s2.0-S221501612400342X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2024-08-05DOI: 10.1016/j.mex.2024.102892
Maricarmen Iñiguez-Moreno , Berenice Santiesteban-Romero , Elda M. Melchor-Martínez , Roberto Parra-Saldívar , Reyna Berenice González-González
{"title":"Valorization of fishery industry waste: Chitosan extraction and its application in the industry","authors":"Maricarmen Iñiguez-Moreno , Berenice Santiesteban-Romero , Elda M. Melchor-Martínez , Roberto Parra-Saldívar , Reyna Berenice González-González","doi":"10.1016/j.mex.2024.102892","DOIUrl":"10.1016/j.mex.2024.102892","url":null,"abstract":"<div><p>Waste from the fishing industry is disposed of in soils and oceans, causing environmental damage. However, it is also a source of valuable compounds such as chitin. Although chitin is the second most abundant polymer in nature, its use in industry is limited due to the lack of standardized and scalable extraction methods and its poor solubility. The deacetylation process increases its potential applications by enabling the recovery of chitosan, which is soluble in dilute acidic solutions. Chitosan is a polymer of great importance due to its biocompatible and bioactive properties, which include antimicrobial and antioxidant capabilities. Chitin extraction and its deacetylation to obtain chitosan are typically performed using chemical processes that involve large amounts of strongly acidic and alkaline solutions. To reduce the environmental impact of this process, extraction methods based on biotechnological tools, such as fermentation and chitin deacetylase, as well as emerging technologies, have been proposed. These extraction methods have demonstrated the potential to reduce or even avoid using strong solvents and shorten extraction time, thereby reducing costs.</p><p>Nevertheless, it is important to address existing gaps in this area, such as the requirements for large-scale implementation and the determination of the stoichiometric ratios for each process. This review highlights the use of biotechnological tools and emerging technologies for chitin extraction and chitosan production. These approaches truly minimize environmental impact, reduce the use of strong solvents, and shorten extraction time. They are a reliable alternative to fishery waste valorization, lowering costs; however, addressing the critical gaps for their large-scale implementation remains challenging.</p></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102892"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003443/pdfft?md5=e7f4c07fbe9a6c02fce9ca6f278f31ef&pid=1-s2.0-S2215016124003443-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141962785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Method for computer tomography voxel-based finite element analysis and validation with digital image correlation system","authors":"Debangshu Paul , Zachariah Arwood , Pierre-Yves Mulon , Dayakar Penumadu , Timothy Truster","doi":"10.1016/j.mex.2024.102879","DOIUrl":"10.1016/j.mex.2024.102879","url":null,"abstract":"<div><p>Understanding the mechanical behavior of heterogeneous materials is becoming increasingly crucial across various fields, including aerospace engineering, composite materials development, geology, and biomechanics. While substantial literature exists on this topic, conventional methods often rely on commercial software packages. This study presents a framework for computed tomography (CT) scan-based finite element (FE) analysis of such materials using open-source software in most of the workflow.</p><p>Our work focuses on three key aspects:</p><ul><li><span>1.</span><span><p>Mesh generation that incorporates spatially varying mechanical properties and well-defined boundary conditions.</p></span></li><li><span>2.</span><span><p>Validation of the FE results through comparison with digital image correlation (DIC) system measurements.</p></span></li><li><span>3.</span><span><p>Open-source software utilization throughout the entire process, making it more accessible and cost-effective.</p></span></li></ul>This work aims to demonstrate the effectiveness of this framework for analyzing heterogeneous materials in various fields, offering a more accessible and affordable approach<em>.</em></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102879"},"PeriodicalIF":1.6,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003315/pdfft?md5=9561774964260789ca3ba702b57c2a0e&pid=1-s2.0-S2215016124003315-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141962786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2024-08-02DOI: 10.1016/j.mex.2024.102882
Pedro Arana-Agudelo, Ioan-Cristian Trelea, Kevin Lachin, Violaine Athès, Marwen Moussa
{"title":"A pycnometric method to estimate the porosity of hydrophobic hollow fiber membranes","authors":"Pedro Arana-Agudelo, Ioan-Cristian Trelea, Kevin Lachin, Violaine Athès, Marwen Moussa","doi":"10.1016/j.mex.2024.102882","DOIUrl":"10.1016/j.mex.2024.102882","url":null,"abstract":"<div><p>The porosity of hollow fiber membranes is an important property in the design of processes implementing membrane contactors as it is directly related to the effective surface available for mass transfer. Nevertheless, measuring the porosity requires most of the time complex experimental setup and some of the existing methods are questionable when applied to polymeric membrane materials. In this work, we adapted a method originally proposed to estimate the porosity of flat membranes, in order to estimate the porosity of hollow fiber membranes.</p><ul><li><span>•</span><span><p>Some hydrophobic hollow fibers are put in contact with a non-wetting solvent inside a pycnometer. The mass of the system is measured.</p></span></li><li><span>•</span><span><p>The process is repeated using a wetting solvent.</p></span></li><li><span>•</span><span><p>The porosity is deduced from the difference between the weighing data.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102882"},"PeriodicalIF":1.6,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003340/pdfft?md5=2e5e32e9e355bf46897a2ae666034fdf&pid=1-s2.0-S2215016124003340-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141962724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}