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A standardized protocol for microplastic and non-synthetic microfiber extraction and µFTIR identification in elasmobranch gastrointestinal tissues 板鳃纲胃肠道组织中微塑料和非合成微纤维提取和微FTIR鉴定的标准化方案
IF 1.9
MethodsX Pub Date : 2026-06-01 Epub Date: 2026-06-06 DOI: 10.1016/j.mex.2026.103996
Roberto Cruz-García, Felipe Amezcua
{"title":"A standardized protocol for microplastic and non-synthetic microfiber extraction and µFTIR identification in elasmobranch gastrointestinal tissues","authors":"Roberto Cruz-García,&nbsp;Felipe Amezcua","doi":"10.1016/j.mex.2026.103996","DOIUrl":"10.1016/j.mex.2026.103996","url":null,"abstract":"<div><div>Elasmobranch gastrointestinal tissues are a complex matrix for microplastic work: high in lipids, dense in connective tissue, and prone to losing small particles during handling. In this work, we developed a protocol that targets these problems. Tissue is digested in 20% (w/v) KOH at 40°C until the soft fraction dissolves (24–72 h); when organic residues persist, 30% (v/v) H₂O₂ is added until the solution clears to amber. The digest is then density-separated in 5 M NaCl, filtered through 1.2 µm glass fiber, sorted under a stereomicroscope, and identified by µFTIR-ATR.</div><div>Validation on eight samples spiked with 100 polyethylene reference particles (50–500 µm) gave recoveries of 90–96% (mean 93.4 ± 2.06%); the procedural blank returned 97%. From the real samples, 172 particles were recovered, mostly fibers, with cellulose as the dominant material, alongside PET, acrylic, nylon, and polypropylene. Detection was reliable down to ∼150 µm by stereomicroscopy and ∼60–70 µm by µFTIR.</div><div>The method is not designed for particles below 50 µm or polymers denser than the saline brine (∼1.2 g cm⁻³); because both require adapting the density step.</div><div>Key features of the method:<ul><li><span>•</span><span><div>Sequential KOH–H₂O₂ digestion handles the fat- and protein-rich matrix of shark and ray digestive tissue without altering common polymers.</div></span></li><li><span>•</span><span><div>Mean recovery of 93.4% on polyethylene-spiked samples (90–96%); 97% in procedural blanks.</div></span></li><li><span>•</span><span><div>µFTIR-ATR identification down to ∼60 µm, with a reliable visual sorting threshold of ∼150 µm.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103996"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148241449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Two-phase drift monitoring and automatic retraining (TPDM-AR) for electric load forecasting 电力负荷预测的两相漂移监测与自动再训练(TPDM-AR)。
IF 1.9
MethodsX Pub Date : 2026-06-01 Epub Date: 2026-05-20 DOI: 10.1016/j.mex.2026.103966
Michel B. Oliveira , Leandro A. Silva
{"title":"Two-phase drift monitoring and automatic retraining (TPDM-AR) for electric load forecasting","authors":"Michel B. Oliveira ,&nbsp;Leandro A. Silva","doi":"10.1016/j.mex.2026.103966","DOIUrl":"10.1016/j.mex.2026.103966","url":null,"abstract":"<div><div>Monitoring incremental concept and data drift is important for electric load forecasting in smart buildings. This study proposes TPDM-AR (Two-Phase Drift Monitoring and Automatic Retraining), a customizable workflow that combines Adaptive Windowing (ADWIN), Kolmogorov–Smirnov Windowing (KSWIN), and Page–Hinkley (PH) drift detectors with a multilayer perceptron (MLP) regressor. Phase 1 monitors the raw active-power stream for distribution shifts, while Phase 2 confirms predictive degradation by tracking mean absolute error (MAE) using a smoothed MAE trajectory compared with a baseline reference threshold defined from a reference window. Retraining is initiated only when Phase 2 satisfies a consensus-and-persistence confirmation rule on the forecasting-error stream, while Phase 1 alerts on the raw series are reported as complementary signals. The method is validated on controlled synthetic linear and logarithmic drift scenarios and on the UCI Individual Household Electric Power Consumption dataset, reporting detector alert times and the corresponding evolution of forecasting error under the same monitoring setup.<ul><li><span>•</span><span><div>Two-phase drift monitoring that combines early change alerts with performance confirmation.</div></span></li><li><span>•</span><span><div>Retraining is triggered only after Phase 2 consensus-and-persistence confirmation on MAE.</div></span></li><li><span>•</span><span><div>Validated on synthetic drift and real-world electricity-demand data.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103966"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148157177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A construction of statistical inferences in geographically weighted univariate log-gamma regression. 地理加权单变量对数回归统计推断的构建。
IF 2.9
MethodsX Pub Date : 2026-05-29 eCollection Date: 2026-06-01 DOI: 10.1016/j.mex.2026.103978
Dyah Setyo Rini, Purhadi, Shofi Andari
{"title":"A construction of statistical inferences in geographically weighted univariate log-gamma regression.","authors":"Dyah Setyo Rini, Purhadi, Shofi Andari","doi":"10.1016/j.mex.2026.103978","DOIUrl":"10.1016/j.mex.2026.103978","url":null,"abstract":"<p><p>Log-gamma regression (LGR) is a non-linear regression model in which the response variable follows a log-gamma distribution. Previous studies have generally applied the two-parameter log-gamma distribution, consisting of shape and scale. This study extends the model by introducing an additional location parameter, forming a three-parameter log-gamma distribution. Since spatial heterogeneity often reduces the accuracy of conventional LGR, we propose the geographically weighted univariate log-gamma regression (GWULGR) to better account for spatial variation in the data. The objective of this study is to develop the GWULGR model, provide a statistical framework for its estimation and testing, and demonstrate its effectiveness through an application to regional life expectancy. The empirical results show that GWULGR provides a substantially better fit than the conventional LGR model, as reflected by a smaller corrected Akaike Information Criterion (AICc) value (162.4875 compared to 209.8250). These findings emphasize the importance of integrating spatial effects into log-gamma regression to capture local variation more accurately. Our approach involves:•Extending log-gamma regression into a three-parameter form.•Incorporating spatial effects using a geographically weighted regression (GWR) framework.•Evaluating model performance through an application to the 2023 life expectancy index across districts and cities in Sumatra.</p>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"103978"},"PeriodicalIF":2.9,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13253080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing nutritional compliance, plate waste and menu acceptability in Spanish school meal programmes (ANPAS-Sp): protocol for an explanatory sequential mixed methods study. 评估西班牙学校膳食计划(ANPAS-Sp)的营养依从性、盘子浪费和菜单可接受性:解释性顺序混合方法研究方案。
IF 2.9
MethodsX Pub Date : 2026-05-28 eCollection Date: 2026-06-01 DOI: 10.1016/j.mex.2026.103975
Alba González-Gallego, Sergi Fàbregues, Victor M González-Chordá, Gemma Chiva-Blanch, Anna Pérez-Giménez, Maria Tarafa Orpinell, Silvia Fernández-Barrés, Maria Blanquer, Carles Ariza, Nadia San Onofre, Anna Bach-Faig
{"title":"Assessing nutritional compliance, plate waste and menu acceptability in Spanish school meal programmes (ANPAS-Sp): protocol for an explanatory sequential mixed methods study.","authors":"Alba González-Gallego, Sergi Fàbregues, Victor M González-Chordá, Gemma Chiva-Blanch, Anna Pérez-Giménez, Maria Tarafa Orpinell, Silvia Fernández-Barrés, Maria Blanquer, Carles Ariza, Nadia San Onofre, Anna Bach-Faig","doi":"10.1016/j.mex.2026.103975","DOIUrl":"10.1016/j.mex.2026.103975","url":null,"abstract":"<p><p>The implementation of healthy, sustainable school menus represents a critical transition requiring strategic management to ensure nutritional compliance, student acceptability, and minimal food waste. Currently, evidence remains inconsistent due to a lack of validated protocols correlating nutritional standards with objective consumption data. Consequently, a comprehensive, explanatory sequential mixed-methods protocol is developed (ANPAS-Sp). It integrates a systematic nutritional audit (Phase 1) -aligned with Spain's Real Decreto 315/2025 and Catalonia's School Menu Review Programme-with high-precision direct weighing of plate waste and validated facial hedonic scales (Phase 2). Furthermore, the protocol employs targeted focus groups to qualitatively explore food rejection patterns identified after quantitative phase (Phase 3). This protocol facilitates evidence-based interventions and data pooling to advance sustainable school food systems. It spearheads a standardized evaluation framework for public health policies, establishing a new benchmark for gathering integrated evidence. Ultimately, this approach provides the necessary tools to transform school meal programs into healthy, widely-accepted, and zero-waste systems.•Approach: A standardised evaluation framework that empirically correlates the compliance of nutritional recommendations, the quantification of plate waste, and student acceptability of school meals.•Impact: It provides the necessary scientific tools to optimise school meal programmes and promote the transition towards sustainable school food systems.</p>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"103975"},"PeriodicalIF":2.9,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148265312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A low-cost approach to dimple grinding using a rotary tool. 一种低成本的使用旋转工具进行凹坑磨削的方法。
IF 2.9
MethodsX Pub Date : 2026-05-27 eCollection Date: 2026-06-01 DOI: 10.1016/j.mex.2026.103971
Hideyuki Magara, Daisuke Morikawa, Kenji Tsuda
{"title":"A low-cost approach to dimple grinding using a rotary tool.","authors":"Hideyuki Magara, Daisuke Morikawa, Kenji Tsuda","doi":"10.1016/j.mex.2026.103971","DOIUrl":"10.1016/j.mex.2026.103971","url":null,"abstract":"<p><p>Conventional transmission electron microscopy (TEM) specimen preparation often relies on expensive commercial dimple grinders. This study evaluates a highly affordable alternative for mechanical thinning, utilizing a consumer-grade rotary tool. This represents a significant cost saving of two orders of magnitude, making TEM preparation much more accessible. The objective is to determine whether such low-cost equipment can produce high-quality samples comparable to those prepared using specialized laboratory devices. The methodology is outlined as follows: A dimple fabrication system, functionally equivalent to conventional commercial products, was developed using an inexpensive rotary tool. TEM specimens were prepared following a dimpling process performed using a standard commercial dimple grinder and the developed rotary tool system (evaluating both low-cost and ultra-low-cost methods). The resulting crystalline quality was quantitatively evaluated using convergent-beam electron diffraction (CBED) and symmetry analysis. Experimental results on single-crystal silicon demonstrate that the proposed low-cost method achieves crystalline quality comparable to the conventional approach. Furthermore, even the ultra-low-cost variation utilizing standard steel bits maintains structural integrity without introducing dislocations or stacking faults. These findings indicate that inexpensive rotary tools offer a practical, highly accessible solution for routine, high-quality TEM specimen preparation.</p>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"103971"},"PeriodicalIF":2.9,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13251799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148226652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A reproducible mechano-thermal injury method for reconstructed human epidermis to study wound re-epithelialization. 重建人表皮的可重复机械热损伤方法研究创面再上皮化。
IF 2.9
MethodsX Pub Date : 2026-05-26 eCollection Date: 2026-06-01 DOI: 10.1016/j.mex.2026.103976
Thaís C Pereira, Victória S Padilha, José M de Brito Neto, Raquel F Chaves, Vanja Dakic, Ronaldo José F C do Amaral
{"title":"A reproducible mechano-thermal injury method for reconstructed human epidermis to study wound re-epithelialization.","authors":"Thaís C Pereira, Victória S Padilha, José M de Brito Neto, Raquel F Chaves, Vanja Dakic, Ronaldo José F C do Amaral","doi":"10.1016/j.mex.2026.103976","DOIUrl":"10.1016/j.mex.2026.103976","url":null,"abstract":"<p><p>Reconstructed human epidermis (RHE) models are widely used as an ethical alternative to animal testing and are validated for cutaneous toxicity assessment. However, their application in wound healing research remains limited due to the lack of standardized methods to induce reproducible epidermal injuries. Generating localized lesions while preserving the polycarbonate membrane required for keratinocyte migration remains a technical challenge. Here we describe a method to generate controlled lesions in reconstructed human epidermis suitable for re-epithelialization studies. Several injury strategies were evaluated, including mechanical approaches using pipette tips, punches, microneedles and glass capillaries. These approaches frequently produced inconsistent lesions or disrupted the membrane. A mechano-thermal strategy proved to be the most reliable approach, generating well-defined and reproducible lesions while preserving membrane integrity. Histological analysis confirmed characteristic features of thermal injury. Longitudinal macroscopic and histological analyses, including semi-quantitative scoring, demonstrated progressive epidermal reorganization following injury. This method establishes a reproducible epidermal burn model suitable for studying epidermal regeneration and wound healing.•Comparative evaluation of mechanical and mechano-thermal strategies to induce lesions in reconstructed human epidermis.•Identification of a reproducible injury method that preserves the polycarbonate membrane required for keratinocyte migration.•Development of a standardized epidermal burn model for re-epithelialization studies.</p>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"103976"},"PeriodicalIF":2.9,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13254972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Benchmark method for selecting FFT windows in harmonic measurements. 谐波测量中FFT窗口选择的基准方法。
IF 1.9
MethodsX Pub Date : 2026-05-25 eCollection Date: 2026-06-01 DOI: 10.1016/j.mex.2026.103972
D C Pham, Tuan Ngoc Anh Nguyen
{"title":"Benchmark method for selecting FFT windows in harmonic measurements.","authors":"D C Pham, Tuan Ngoc Anh Nguyen","doi":"10.1016/j.mex.2026.103972","DOIUrl":"https://doi.org/10.1016/j.mex.2026.103972","url":null,"abstract":"<p><p>The paper presents a reproducible and measurement-oriented benchmark for selecting existing FFT windows in harmonic measurements under short-duration conditions. Candidate windows are assessed using amplitude-related error, THD deviation, and cross-case consistency. Their overall performance is then summarized through a direct quantitative ranking. Validation is carried out using APS1000-based records under waveform-varying, off-nominal, and noise-contaminated conditions. The main contributions of this work are as follows:•A measurement-oriented performance formulation is established for FFT-window evaluation using harmonic-related error, THD deviation, and cross-case consistency as the main comparison criteria.•A direct quantitative window-selection rule is proposed by combining these criteria into an aggregate score, from which the most suitable window is identified under the considered measurement conditions.•The proposed method is implemented in MATLAB and validated using APS1000-based records, including noise-augmented cases generated in MATLAB, under representative harmonic-measurement conditions.</p>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"103972"},"PeriodicalIF":1.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13235378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A structured and embodied framework (SAEF) for pain communication and externalization. 一个结构化和具体化的框架(SAEF)的疼痛沟通和外化。
IF 2.9
MethodsX Pub Date : 2026-05-25 eCollection Date: 2026-06-01 DOI: 10.1016/j.mex.2026.103973
Asmath Asina M, R K Jaishree Karthiga
{"title":"A structured and embodied framework (SAEF) for pain communication and externalization.","authors":"Asmath Asina M, R K Jaishree Karthiga","doi":"10.1016/j.mex.2026.103973","DOIUrl":"10.1016/j.mex.2026.103973","url":null,"abstract":"<p><p>Pain with physiological or psychological distress remains unexpressed because of emotional barriers, cognitive stigma and sensory strain. Due to an insufficient psychosocial or supportive care resources, people find it difficult to express their pain experiences in words, resulting in miscommunication and mental turmoil (Scarry, 1985). Existing models of pain articulation therapies rely more on verbal and narrative modes of expression than on innovative non-verbal approaches. This study presents a Structured And Embodied Framework <b>(SAEF)</b> for pain communication and externalization grounded in Embodied Cognition Theory, Interoception Theory, and Cognitive Behavioral Theory. The framework externalizes and communicates pain through a structured theatrical model involving character embodiment, gesture-based expression, emotional improvisation, and metaphorical performances for pain expression and emotional regulation, without disclosing the cause.•Theconceptual framework helps to communicate and express pain in people, moving them from the patient to participant phase.•The framework is an interdisciplinary exploratory tool that combines clinical psychology and applied theatre practice.•This framework is useful for therapists, theatre practitioners, clinicians, caregivers, and people in art-based avenues.</p>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"103973"},"PeriodicalIF":2.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13255038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolomics and ultra-processed foods intake: A scoping review protocol. 代谢组学和超加工食品摄入:一项范围审查方案。
IF 2.9
MethodsX Pub Date : 2026-05-25 eCollection Date: 2026-06-01 DOI: 10.1016/j.mex.2026.103974
Talita do Nascimento Peixoto, Anna Luisa Moura Alencar Rocha, Lucia Fátima Campos Pedrosa
{"title":"Metabolomics and ultra-processed foods intake: A scoping review protocol.","authors":"Talita do Nascimento Peixoto, Anna Luisa Moura Alencar Rocha, Lucia Fátima Campos Pedrosa","doi":"10.1016/j.mex.2026.103974","DOIUrl":"10.1016/j.mex.2026.103974","url":null,"abstract":"<p><p>The association between the consumption of ultra-processed foods (UPFs) and adverse health outcomes remains unclear regarding its mechanistic explanation. In this context, metabolomics emerges as a promising approach for identifying biomarkers and understanding the metabolic effects of these foods on the body. However, the evidence linking metabolomic profiles to UPFs consumption remains limited and heterogeneous. This scoping review aims to map and synthesize the available scientific evidence on the use of metabolomics in the study of UPFs consumption, characterize the study designs and methodological approaches employed, and identify knowledge gaps. The review will be conducted according to the JBI methodology and reported in accordance with the PRISMA-ScR checklist. Searches will be performed in the PubMed/MEDLINE, Embase, LILACS and Web of Science databases. Two reviewers will independently screen titles, abstracts, and full-text articles for inclusion. Data will be extracted using a standardized form and synthesized narratively. This review is expected to provide a comprehensive overview of the available evidence, highlight methodological heterogeneity, and identify gaps that may guide future research. The protocol was registered in the Open Science Framework (OSF).</p>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"103974"},"PeriodicalIF":2.9,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13235362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Markerless 3D hand tracking for analysis of pediatric eye-hand coordination. 无标记3D手跟踪分析儿童眼手协调。
IF 2.9
MethodsX Pub Date : 2026-05-23 eCollection Date: 2026-06-01 DOI: 10.1016/j.mex.2026.103965
Anjali Rajkumar, Rajvardhan Gadde, Mahya Beheshti, Renat Sukhov, John-Ross Rizzo, Todd E Hudson
{"title":"Markerless 3D hand tracking for analysis of pediatric eye-hand coordination.","authors":"Anjali Rajkumar, Rajvardhan Gadde, Mahya Beheshti, Renat Sukhov, John-Ross Rizzo, Todd E Hudson","doi":"10.1016/j.mex.2026.103965","DOIUrl":"10.1016/j.mex.2026.103965","url":null,"abstract":"<p><p>Precise quantification of eye-hand coordination (EHC) during pediatric dexterity tasks is limited by the lack of practical, high-resolution hand tracking methods suitable for children with brain injury or neurodegenerative disease. Traditional marker-based motion capture systems and instrumented gloves can interfere with natural grasp patterns and are often difficult to implement in clinical pediatric settings. We describe an adaptation of the Anipose markerless 3D pose estimation framework to enable synchronized three-dimensional hand kinematics and eye tracking during the Nine-Hole Peg Test (9HPT). The method integrates multi-camera video acquisition with task-specific neural network training optimized to detect fine finger movements across diverse pediatric hand sizes and grasp configurations. Camera placement and recording geometry were configured to reduce occlusion during peg manipulation and maintain multi-view visibility of hand landmarks. Model validation demonstrated low pixel error and stable three-dimensional reconstruction following confidence-based thresholding. The resulting workflow generates synchronized 2D and 3D visualizations, spatial coordinate outputs, reprojection-error metrics, and landmark confidence scores without requiring wearable sensors. This approach broadens the applicability of eye-hand coordination research within pediatric clinical populations and facilitates the development of more precise, quantitatively informed diagnostic assessments and targeted neurorehabilitation strategies for children with neurologic injury. • Markerless multi-camera 3D reconstruction of pediatric hand kinematics during the 9HPT • Integration of synchronized eye tracking and task-specific neural network training • Output of validated 3D coordinates, confidence metrics, and visualization files suitable for clinical research.</p>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"103965"},"PeriodicalIF":2.9,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13231014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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