MethodsX最新文献

筛选
英文 中文
IoT-enabled effective real-time water quality monitoring method for aquaculture 物联网支持的有效水产养殖水质实时监测方法
IF 1.6
MethodsX Pub Date : 2024-08-13 DOI: 10.1016/j.mex.2024.102906
Rupali P. Shete , Anupkumar M. Bongale , Deepak Dharrao
{"title":"IoT-enabled effective real-time water quality monitoring method for aquaculture","authors":"Rupali P. Shete ,&nbsp;Anupkumar M. Bongale ,&nbsp;Deepak Dharrao","doi":"10.1016/j.mex.2024.102906","DOIUrl":"10.1016/j.mex.2024.102906","url":null,"abstract":"<div><p>Aquaculture is growing industry from the perspective of sustainable food fulfillment and county's economic development. Technology oriented aquafarming is the solution for effective water quality monitoring and high yield production. Internet of Things (IoT) integrated aquaculture can cater to such requirements. This research article introduces a comprehensive method aimed at seamlessly incorporate IoT sensors into aquafarming environments, utilizing Arduino boards and communication modules. The proposed method measures accurate water quality parameters, such as temperature, pH levels, and Dissolved Oxygen (DO), which are essential for maintaining optimal conditions for suitable aquaculture environment. This method enables the real-time collection of critical data points that are essential prevent fish diseases and mortality with low human intervention and maintenance cost. The key contributions of the methodology are mentioned below.</p><ul><li><span>•</span><span><p>Design and development of a compact and efficient Printed Circuit Board (PCB) to achieve accurate sensor data readings and reliable communication in an aqua environment.</p></span></li><li><span>•</span><span><p>Prevent fish disease and mortality rate through data-driven decision incorporating correlation of DO, pH, and temperature sensor data.</p></span></li><li><span>•</span><span><p>Conducted instrument calibration checks and cross-validated automated system data with manual observations through repeatability tests to ensure precise measurements of sensor parameters.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102906"},"PeriodicalIF":1.6,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003583/pdfft?md5=6baef658e02e303d8a2971d157b42766&pid=1-s2.0-S2215016124003583-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple method to assess flood regulation supply in urban lawns 评估城市草坪洪水调节供应的简单方法
IF 1.6
MethodsX Pub Date : 2024-08-13 DOI: 10.1016/j.mex.2024.102905
Paulo Pereira , Miguel Inacio , Marius Kalinauskas , Luis Pinto , Damia Barcelo , Igor Bogunovic
{"title":"A simple method to assess flood regulation supply in urban lawns","authors":"Paulo Pereira ,&nbsp;Miguel Inacio ,&nbsp;Marius Kalinauskas ,&nbsp;Luis Pinto ,&nbsp;Damia Barcelo ,&nbsp;Igor Bogunovic","doi":"10.1016/j.mex.2024.102905","DOIUrl":"10.1016/j.mex.2024.102905","url":null,"abstract":"<div><p>Floods have an important impact on life and loss of goods. Urban green spaces are crucial to mitigating flood impact. However, their capacity to prevent floods depends on their condition, especially in areas highly affected by human activities such as lawns. Here, we developed a simple method to assess flood regulation using soil penetration resistance as a proxy and tested it on an urban lawn in Vilnius (Lithuania) in winter. We developed an experimental design using an app for collecting data and working with it in a GIS environment. To understand their spatial relations, geostatistical (e.g., semi-variogram model and ordinary kriging mapping) and spatial statistics ((Moran's global autocorrelation index and Cluster and Outlier Analysis (Anselin Local Moran's I)) tools were applied. The preliminary results from the tested method showed that the lawn studied has different capacities to retain floods due to the management practices. Nevertheless, it is essential to be applied in different soil moisture conditions since flood regulation (soil penetration resistance) can be variable throughout the year.</p><ul><li><span>•</span><span><p>A novel method was developed to estimate flood regulation using soil penetration resistance as a proxy;</p></span></li><li><span>•</span><span><p>An urban lawn was used to test the method and identify areas with low and high capacity for flood regulation;</p></span></li><li><span>•</span><span><p>The method quickly assesses lawn flood retention capacity in different environments.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102905"},"PeriodicalIF":1.6,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003571/pdfft?md5=961075575de202e08efe9a8645030e66&pid=1-s2.0-S2215016124003571-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142040683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatial clustering based on geographically weighted multivariate generalized gamma regression 基于地理加权多变量广义伽马回归的空间聚类法
IF 1.6
MethodsX Pub Date : 2024-08-10 DOI: 10.1016/j.mex.2024.102903
Hasbi Yasin , Purhadi , Achmad Choiruddin
{"title":"Spatial clustering based on geographically weighted multivariate generalized gamma regression","authors":"Hasbi Yasin ,&nbsp;Purhadi ,&nbsp;Achmad Choiruddin","doi":"10.1016/j.mex.2024.102903","DOIUrl":"10.1016/j.mex.2024.102903","url":null,"abstract":"<div><p>Geographically Weighted Regression (GWR) is one of the local statistical models that can capture the effects of spatial heterogeneity. This model can be used for both univariate and multivariate responses. However, it should be noted that GWR models require the assumption of error normality. To overcome this problem, we propose a GWR model for generalized gamma distributed responses that can capture the phenomenon of some special continuous distributions. The proposed model is known as Geographically Weighted Multivariate Generalized Gamma Regression (GWMGGR). Parameter estimation is performed using the Maximum Likelihood Estimation (MLE) method optimized with the Bernt-Hall-Hall-Haussman (BHHH) algorithm. To determine the significance of the spatial heterogeneity effect, a hypothesis test was conducted using the Maximum Likelihood Ratio Test (MLRT) approach. We made a spatial cluster based on the estimated model parameters for each response using the k-means clustering method to interpret the obtained results. Some highlights of the proposed method are:</p><ul><li><span>•</span><span><p>A new model for GWR with multivariate generalized gamma distributed responses to overcome the assumption of normally distributed errors.</p></span></li><li><span>•</span><span><p>Goodness of fit test to test the spatial effects in GWMGGR model.</p></span></li><li><span>•</span><span><p>Spatial clustering of districts/cities in Central Java based on three dimensions of educational indicators.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102903"},"PeriodicalIF":1.6,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003558/pdfft?md5=493ec9695f34abf11d7f0e6beb6efdd0&pid=1-s2.0-S2215016124003558-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141978095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preparing eugregarine parasites and their cricket host Acheta domesticus as a model for gregarine infection studies 准备将蛞蝓寄生虫及其宿主蟋蟀(Acheta domesticus)作为蛞蝓感染研究的模型
IF 1.6
MethodsX Pub Date : 2024-08-10 DOI: 10.1016/j.mex.2024.102888
Edouard Bessette , Bryony A.P. Williams , Nicolai V. Meyling
{"title":"Preparing eugregarine parasites and their cricket host Acheta domesticus as a model for gregarine infection studies","authors":"Edouard Bessette ,&nbsp;Bryony A.P. Williams ,&nbsp;Nicolai V. Meyling","doi":"10.1016/j.mex.2024.102888","DOIUrl":"10.1016/j.mex.2024.102888","url":null,"abstract":"<div><p>The increasing global demand for house crickets (<em>Acheta domesticus</em>) necessitates effective health measures. Despite their abundance, the effects of gregarines on these hosts remain underexplored. We present a method for controlled gregarine infection and maintenance of gregarine-free cricket populations. This system, adapted from prior work, is essential for experiments on gregarine infection dynamics, providing insights into parasite evolution and host-parasite interactions within the Apicomplexa group. This protocol includes rearing and maintenance of gregarine-free cricket populations for experimental purposes, gregarine production for oocyst solution and cricket infection, and gregarine infection assessment.</p></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102888"},"PeriodicalIF":1.6,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003406/pdfft?md5=ab81073bbe88371ad9d5b8e42777809b&pid=1-s2.0-S2215016124003406-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141964338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved and easy method for long-term in vitro culture of rumen ciliates Dasytricha ruminantium 瘤胃纤毛虫 Dasytricha ruminantium 长期体外培养的改进型简便方法
IF 1.6
MethodsX Pub Date : 2024-08-10 DOI: 10.1016/j.mex.2024.102902
Qinhui Xu , Jinying He , Yujia Wang , Jie Xiong , Weiwei Qin , Jinmei Feng
{"title":"Improved and easy method for long-term in vitro culture of rumen ciliates Dasytricha ruminantium","authors":"Qinhui Xu ,&nbsp;Jinying He ,&nbsp;Yujia Wang ,&nbsp;Jie Xiong ,&nbsp;Weiwei Qin ,&nbsp;Jinmei Feng","doi":"10.1016/j.mex.2024.102902","DOIUrl":"10.1016/j.mex.2024.102902","url":null,"abstract":"<div><p>The rumen ciliates are a diverse group of protozoa residing in the rumen of ruminant animals. They are primarily found in the orders Entodiniomorphida and Vestibuliferida, playing crucial roles in the digestion and breakdown of feed within the host's rumen, closely intertwined with the host's nutrient absorption. <em>In vitro</em> monocultures of representatives of rumen ciliates are important to better study them. So far, <em>Entodinium caudatum</em> and <em>Epidinium caudatum</em>, representatives of the order Entodiniomorphida, have been successfully cultivated as a monoculture <em>in vitro</em>. However, for the order Vestibuliferida, no representative species has been established a stable monoculture <em>in vitro</em> up to date, which hampers to study their physiology and metabolism. Therefore, we have developed a simple method for the <em>in vitro</em> cultivation of <em>Dasytricha ruminantium</em>, a representative rumen ciliate in the order Vestibuliferida. Utilizing an optimized culture medium with easily obtainable components, and the cultivation process is simple. This will facilitate further research in metabolism and other studies requiring large pure live materials.</p><ul><li><span>1.</span><span><p>Filtration and separation for enriching <em>D. ruminantium</em>.</p></span></li><li><span>2.</span><span><p>A culture medium (DRM) suitable for the growth of <em>D. ruminantium</em>, with easily obtainable components.</p></span></li><li><span>3.</span><span><p>Simple cultivation process, facilitating the obtainment of a large number of monocultured <em>D. ruminantium</em>.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102902"},"PeriodicalIF":1.6,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003546/pdfft?md5=c62e7fff035166b7f497581d6df65ae5&pid=1-s2.0-S2215016124003546-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141993831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrating musealized archaeological sediment collections into current geoarchaeological analytical frameworks for sustainable research practices 将博物馆化的考古沉积物藏品纳入当前的地质考古分析框架,促进可持续研究实践
IF 1.6
MethodsX Pub Date : 2024-08-09 DOI: 10.1016/j.mex.2024.102897
Stefano Costanzo , Marta Pappalardo , Elisabetta Starnini , Elena Rossoni-Notter , Olivier Notter , Abdelkader Moussous , Miguel Soares-Remiseiro , Paola Fermo , Mauro Cremaschi , Andrea Zerboni
{"title":"Integrating musealized archaeological sediment collections into current geoarchaeological analytical frameworks for sustainable research practices","authors":"Stefano Costanzo ,&nbsp;Marta Pappalardo ,&nbsp;Elisabetta Starnini ,&nbsp;Elena Rossoni-Notter ,&nbsp;Olivier Notter ,&nbsp;Abdelkader Moussous ,&nbsp;Miguel Soares-Remiseiro ,&nbsp;Paola Fermo ,&nbsp;Mauro Cremaschi ,&nbsp;Andrea Zerboni","doi":"10.1016/j.mex.2024.102897","DOIUrl":"10.1016/j.mex.2024.102897","url":null,"abstract":"<div><p>We present a review of the latest framework achievements in geoarchaeological sciences applied to microstratigraphic and biomolecular studies of prehistoric archaeological contexts, highlighting the importance of musealized archaeological stratigraphies. We assess how today's scientific and technological accomplishments can be tailored for archaeological human ecology studies with analytical ensembles that provide unprecedented results. Sampling and processing workflows originating from resin consolidation and thin section micromorphology of undisturbed blocks of archaeological soils and sediments, guarantee subsampling accuracy at the micrometre scale granting access to individual components otherwise impossible to target: the achievable information yield makes even the smallest soil samples potential sources of pioneering discoveries. Yet, archaeological excavations are still the primary mode of retrieving new soil samples. We argue that, when dealing with archaeological sites that were excavated and partially musealised in the past, the exploration of related museum collections should be prioritized as perspective source of new study samples. Analysing old and potentially very informative samples, with an approach that we define as “Green Archaeology”, may represent a source of well-structured primary data as well as a means for planning new excavations, delivering novel discoveries while safeguarding site integrity and promoting Third Mission valorisation of sites and heritage dormant collections.</p></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102897"},"PeriodicalIF":1.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003492/pdfft?md5=4ecee528752df05f61f1c3d5a57313fb&pid=1-s2.0-S2215016124003492-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141979390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mindfulness and compassion-based programs on eating behavior of post-bariatric surgery patients: A two phased clinical trial protocol 对减肥手术后患者饮食行为的正念和同情计划:两阶段临床试验方案
IF 1.6
MethodsX Pub Date : 2024-08-09 DOI: 10.1016/j.mex.2024.102885
Erika Blamires S. Porto , Jesus Montero-Marin , Luiz Gustavo Quadros , Jean Kristeller , Vicente Sarubbi Junior , Luis Augusto Mattar , Javier Garcia-Campayo , Marcelo Demarzo
{"title":"Mindfulness and compassion-based programs on eating behavior of post-bariatric surgery patients: A two phased clinical trial protocol","authors":"Erika Blamires S. Porto ,&nbsp;Jesus Montero-Marin ,&nbsp;Luiz Gustavo Quadros ,&nbsp;Jean Kristeller ,&nbsp;Vicente Sarubbi Junior ,&nbsp;Luis Augusto Mattar ,&nbsp;Javier Garcia-Campayo ,&nbsp;Marcelo Demarzo","doi":"10.1016/j.mex.2024.102885","DOIUrl":"10.1016/j.mex.2024.102885","url":null,"abstract":"<div><p>Introduction: Weight regain after bariatric surgery remains a relevant and worrisome topic, requiring greater understanding and involvement in research into new adjuvant treatments. This study aims to compare the preliminary effectiveness and feasibility of the Mindfulness-Based Health Promotion and Attachment-Based Compassion Therapy programs as opposed to usual treatments (workshops) on the eating behavior of patients with progressive weight gain after bariatric surgery in Brazilian patients at a private clinic. It was hypothesized that both interventions are feasible and that the self-compassion program may be more effective than the mindfulness program. Methods: The study will be divided into two phases: a cross-analytical study of those who underwent bariatric surgery and a randomized controlled trial only with the ones who had weight regain. Interventions will be conducted for eight weeks synchronously with three assessment points (baseline, post intervention, and 6-month follow-up), both online. The primary outcome will be a change in eating behavior. Secondary outcomes will include improved quality of life, enhanced body image satisfaction and reduced distortion (Brazilian Silhouette Scales for adults), better weight management (maintenance or weight reduction), increased frequency of activity and monitoring with the surgery team. Qualitative data will also be collected by online identification of a sub-sample of participants. Results: Improvements are expected in eating behavior, weight, reverse progressive weight gain, classification of self-image, quality of life, and levels of mindfulness, self-compassion, and anxiety. Conclusion: This study seeks to gather preliminary evidence on the effectiveness of mindfulness and compassion training for the adjunctive treatment of progressive weight gain in post-bariatric patients.</p><p><em>Clinical Trials.gov</em> Registration ID: NCT04171713.</p></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102885"},"PeriodicalIF":1.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003376/pdfft?md5=897a7d4fabcc2917ee683d6d6667da22&pid=1-s2.0-S2215016124003376-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142040687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Challenges in illumination analysis and design for Martian surface-level habitation 火星表面居住照明分析和设计所面临的挑战
IF 1.6
MethodsX Pub Date : 2024-08-08 DOI: 10.1016/j.mex.2024.102898
Kasra Amini , Sana Rastegar , Ehsan Dehghani Janabadi
{"title":"Challenges in illumination analysis and design for Martian surface-level habitation","authors":"Kasra Amini ,&nbsp;Sana Rastegar ,&nbsp;Ehsan Dehghani Janabadi","doi":"10.1016/j.mex.2024.102898","DOIUrl":"10.1016/j.mex.2024.102898","url":null,"abstract":"<div><p>It is needless to say that travel to and settlement on Mars are associated with extreme levels of scientific and engineering issues. This will only be amplified with the long-term duration of the mission, not only due to scarcity of resources, but also as the psychological aspects of the dynamics among the crew increase drastically. It should be emphasized that this is a scientific crew, who have undergone high levels of confinement during space travel to Mars, O (10<sup>2</sup> Earth days), are living in semi-solitude and partial confinement conditions for durations of O (10<sup>3</sup> Earth days), and even at the nominal termination of the mission, foresee a high-risk and arduous travel time of O (10<sup>2</sup> Earth days) back to the Earth. The mental weight of the described mission with its slow pace and tardy episodes, puts the crew under severe psychological issues. Minimal and conservative design of spaces, lack of constant access to the exterior, and social solitude are among major factors contributing to the psychological well-being of the crew. Furthermore, the overall lower levels of natural light, accompanied by the minimum possible area of transparent facades, protecting the crew from harmful radiations and cold exterior, burden the mental conditions of the crew even more. Given the limited availability of data from the surface of Mars, study of the effects linked to the lighting and illumination design of the habitats is challenging. The current manuscript hopes to shed light on the illumination and lighting design and simulation procedure, required data, assumptions, and final results for the surface-level habitats on Mars.</p><ul><li><span>•</span><span><p>Mars / Sub orbital configuration allows for limited natural lighting, however, upon site-specific analysis, it might be considerable as a base passive source.</p></span></li><li><span>•</span><span><p>Current simulation tools are design based on Earth-bound design requirements. These need to be re-oriented to match available planetary data.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102898"},"PeriodicalIF":1.6,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003509/pdfft?md5=a6cb63e79b90ced700f91396a46b6861&pid=1-s2.0-S2215016124003509-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141991110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Real-time sign language detection: Empowering the disabled community 实时手语检测:增强残疾人群体的能力
IF 1.6
MethodsX Pub Date : 2024-08-08 DOI: 10.1016/j.mex.2024.102901
Sumit Kumar , Ruchi Rani , Ulka Chaudhari
{"title":"Real-time sign language detection: Empowering the disabled community","authors":"Sumit Kumar ,&nbsp;Ruchi Rani ,&nbsp;Ulka Chaudhari","doi":"10.1016/j.mex.2024.102901","DOIUrl":"10.1016/j.mex.2024.102901","url":null,"abstract":"<div><p>Interaction and communication for normal human beings are easier than for a person with disabilities like speaking and hearing who may face communication problems with other people. Sign Language helps reduce this communication gap between a normal and disabled person. The prior solutions proposed using several deep learning techniques, such as Convolutional Neural Networks, Support Vector Machines, and K-Nearest Neighbors, have either demonstrated low accuracy or have not been implemented as real-time working systems. This system addresses both issues effectively. This work extends the difficulties faced while classifying the characters in Indian Sign Language(ISL). It can identify a total of 23 hand poses of the ISL. The system uses a pre-trained VGG16 Convolution Neural Network(CNN) with an attention mechanism. The model's training uses the Adam optimizer and cross-entropy loss function. The results demonstrate the effectiveness of transfer learning for ISL classification, achieving an accuracy of 97.5 % with VGG16 and 99.8 % with VGG16 plus attention mechanism.</p><ul><li><span>•</span><span><p>Enabling quick and accurate sign language recognition with the help of trained model VGG16 with an attention mechanism.</p></span></li><li><span>•</span><span><p>The system does not require any external gloves or sensors, which helps to eliminate the need for physical sensors while simplifying the process with reduced costs.</p></span></li><li><span>•</span><span><p>Real-time processing makes the system more helpful for people with speaking and hearing disabilities, making it easier for them to communicate with other humans.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102901"},"PeriodicalIF":1.6,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003534/pdfft?md5=f4ed0ed2c2e051e0fcb53b858a504e61&pid=1-s2.0-S2215016124003534-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141993833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A reliable method to prepare milligram size environmental samples to quantify metal(loid)s by high-resolution graphite furnace atomic absorption spectrometry 制备毫克级环境样品的可靠方法,可通过高分辨率石墨炉原子吸收光谱法量化金属(惰性)物质
IF 1.6
MethodsX Pub Date : 2024-08-08 DOI: 10.1016/j.mex.2024.102896
Patrícia Gomes Costa, Juliana Lemos Dal Pizzol, Yuri Dornelles Zebral, Adalto Bianchini
{"title":"A reliable method to prepare milligram size environmental samples to quantify metal(loid)s by high-resolution graphite furnace atomic absorption spectrometry","authors":"Patrícia Gomes Costa,&nbsp;Juliana Lemos Dal Pizzol,&nbsp;Yuri Dornelles Zebral,&nbsp;Adalto Bianchini","doi":"10.1016/j.mex.2024.102896","DOIUrl":"10.1016/j.mex.2024.102896","url":null,"abstract":"<div><p>We searched for an extraction method that would allow a precise quantification of metal(loid)s in milligram-size samples using high-resolution graphite furnace atomic absorption spectrometry (HR-GFAAS). We digested biological (DORM-4, DOLT-5 and TORT-3) and sediment (MESS-4) certified reference materials (CRMs) using nitric acid in a drying oven, aqua regia in a drying oven, or nitric acid in a microwave. In addition, we digested MESS-4 using a mixture of nitric and hydrofluoric acids in a drying oven. We also evaluated the effect of sample size (100 and 200 mg) on the extraction efficiency. Nitric acid extraction in a drying oven yielded the greatest recovery rates for all metal(loid)s in all tested CRMs (80.0 %–100.0 %) compared with the other extraction methods tested (67.3 %–99.2 %). In most cases, the sample size did not have a significant effect on the extraction efficiency. Therefore, we conclude that nitric acid digestion in a drying oven is a reliable extraction method for milligram-size samples to quantify metal(loid)s with HR-GFAAS. This validated method could provide substantial benefits to environmental quality monitoring programs by significantly reducing the time and costs required for sample collection, storage, transport and preparation, as well as the amount of hazardous chemicals used during sample extraction and analysis.</p><p></p><ul><li><span>•</span><span><p>Sample digestion with nitric acid in a drying oven yielded the greatest recovery rates of metal(loid)s from biological and sediment certified reference materials.</p></span></li><li><span>•</span><span><p>The recovery rates of metal(loid)s from biological and sediment certified reference materials using nitric acid digestion in a drying oven ranged from 73 % to 100 %.</p></span></li><li><span>•</span><span><p>Digestion with nitric acid in a drying oven is a simple and reliable method to extract small size environmental samples for metal(loid)s quantification by high-resolution graphite furnace atomic absorption spectrometry.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102896"},"PeriodicalIF":1.6,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003480/pdfft?md5=b76f6c496dae42d2cf20a4edf24a4e9e&pid=1-s2.0-S2215016124003480-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141964337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信