在11.7T时使用非对比MRI定量评估小鼠脑代谢

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX Pub Date : 2025-01-16 DOI:10.1016/j.mex.2025.103175

Xiuli Yang, Yuguo Li, Hanzhang Lu, Zhiliang Wei

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引用次数: 0

摘要

脑氧代谢表明能量消耗的速度，是病理变化的潜在标志。正电子发射断层扫描（PET）是使用放射性示踪剂测量代谢率的金标准。然而，它在临床前研究中的应用，特别是在啮齿动物身上的应用，受到动脉输入功能测量和现场回旋加速器设备用于示踪剂制备的限制。作为一种替代方法，非侵入性、非造影剂MRI技术，如t2 - relax- underspin -tagging （TRUST）和phase-contrast (PC) MRI，可用于评估体内脑代谢。本研究概述了在11.7T扫描仪下对小鼠进行TRUST和PC MRI的逐步方法。该方法在大约20分钟内产生无创、非对比的全脑代谢定量测量，为未来病理生理学研究的更广泛应用铺平了道路。•小鼠脑代谢的非侵入性和非对比评估。•在大约20分钟内定量测量代谢率。

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Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T

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Quantitative assessment of brain metabolism in mice using non-contrast MRI at 11.7T

Brain oxygen metabolism indicates the rate of energy consumption and is a potential marker of pathological changes. Positron emission tomography (PET) is the gold standard for measuring metabolic rates using radioactive tracers. However, its application in preclinical studies, particularly with rodent animals, is constrained by the need for arterial input function measurements and on-site cyclotron facilities for tracer preparation. As an alternative, non-invasive, non-contrast MRI techniques, such as T2-relaxation-under-spin-tagging (TRUST) and phase-contrast (PC) MRI, can be used for evaluating brain metabolism in vivo. This study outlines a step-by-step method for implementing TRUST and PC MRI in mice at 11.7T scanner. The proposed method yields non-invasive, non-contrast quantitative measurements of global brain metabolism in approximately 20 min, paving the way for broader applications in future pathophysiological studies.