MethodsXPub Date : 2026-06-01Epub Date: 2025-12-19DOI: 10.1016/j.mex.2025.103778
Farooq Ahmed Shah , Iftikhar Haider , Muhammad Waseem , Alexey Mikhaylov , Nora Baranyai
{"title":"Construction and applications of iterative methods for finding approximate solutions of nonlinear equations having unknown zeros of multiplicity with fractal geometry and dynamical behavior","authors":"Farooq Ahmed Shah , Iftikhar Haider , Muhammad Waseem , Alexey Mikhaylov , Nora Baranyai","doi":"10.1016/j.mex.2025.103778","DOIUrl":"10.1016/j.mex.2025.103778","url":null,"abstract":"<div><div>In this study, several new iterative schemes are developed to compute the multiple zeros of nonlinear equations. The construction of these methods is based on the variational iteration approach which provides a systematic framework for formulating efficient and flexible algorithms. The proposed schemes generalize and encompass well-known classical methods such as Newton’s and Halley’s methods, along with their modified versions as special cases. This generality enhances their adaptability to a broader class of nonlinear problems involving both known and unknown multiplicities. To assess the effectiveness of the proposed iterative schemes, extensive numerical experiments are conducted, comparing their convergence speed and accuracy with existing methods. The results demonstrate that the newly developed methods exhibit superior performance in terms of stability, precision, and computational efficiency. Furthermore, to visualize and analyse the global convergence behaviour fractal basin plots are presented. These fractals illustrate the basins of attraction in the complex plane, providing deeper insight into the dynamical behaviour, convergence regions and boundary structures associated with each iterative process.<ul><li><span>•</span><span><div>Developing efficient and flexible iterative methods using the variational iteration method.</div></span></li><li><span>•</span><span><div>Generalizing classical methods to tackle nonlinear problems with known and unknown multiplicity.</div></span></li><li><span>•</span><span><div>Validating performance of various methods through detailed numerical experiments and fractal basin plots.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103778"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2026-06-01Epub Date: 2025-12-16DOI: 10.1016/j.mex.2025.103771
Luca Colnaghi , Giulia Villa , Ilaria Marcomini , Andrea Poliani , Maya Fedeli , Claudio Losi , Debora Rosa , Duilio Fiorenzo Manara
{"title":"Knowledge, support, and networking for Phelan-McDermid syndrome: a study protocol","authors":"Luca Colnaghi , Giulia Villa , Ilaria Marcomini , Andrea Poliani , Maya Fedeli , Claudio Losi , Debora Rosa , Duilio Fiorenzo Manara","doi":"10.1016/j.mex.2025.103771","DOIUrl":"10.1016/j.mex.2025.103771","url":null,"abstract":"<div><h3>Background</h3><div>Phelan-McDermid syndrome (PMS) is a rare neurodevelopmental disorder caused by deletions in chromosome 22q13.3 or pathogenic variants in the SHANK3 gene. Individuals present with intellectual disability, autism-spectrum traits, seizures, gastrointestinal and motor issues, and sleep disturbances, requiring lifelong multidisciplinary care. In Italy, PMS care is fragmented and unevenly distributed, with families often providing intensive home-based support at high personal, financial, and social costs.</div></div><div><h3>Methods</h3><div>This national participatory Citizen-Science study, conducted with the Italian Phelan-McDermid Syndrome Association (AISPHEM), will engage informal caregivers of individuals with genetically confirmed PMS across Italy. A qualitative phase using semi-structured online interviews will explore caregiving experiences, unmet needs, barriers to care, coping strategies, and social isolation. Insights will guide the creation of the first Italian PMS registry, capturing longitudinal clinical, socio-demographic, and caregiver-related data.</div></div><div><h3>Expected Results & Conclusions</h3><div>The project will generate novel evidence on caregiver needs, develop the first national PMS registry, and produce a service map to support equitable, coordinated PMS care and a stronger national caregiver network in Italy.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103771"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2026-06-01Epub Date: 2026-01-09DOI: 10.1016/j.mex.2026.103794
Thuan Ha, Kwabena Abrefa Nketia, Hansanee Fernando, Sarah van Steenbergen, Shawn Neudorf, Steve J. Shirtliffe
{"title":"Field boundary delineation with seasonal sentinel 2 imagery using Segment Anything Model (SAM)","authors":"Thuan Ha, Kwabena Abrefa Nketia, Hansanee Fernando, Sarah van Steenbergen, Shawn Neudorf, Steve J. Shirtliffe","doi":"10.1016/j.mex.2026.103794","DOIUrl":"10.1016/j.mex.2026.103794","url":null,"abstract":"<div><div>Accurate field boundary delineation is critical for accurate modelling on crop yields and for precision agriculture (PA), enabling site-specific management to optimize resource use and crop productivity. Traditional boundary mapping methods, such as manual digitization and semi-automated extraction from farm machinery, are labor-intensive and challenging to apply at large scales. Advances in high-resolution land cover data and satellite imagery offer scalable solutions for automated field boundary extraction. In this study, we propose a fully automated workflow that integrates a pre-trained foundation model, the Segment Anything Model - SAM [<span><span>1</span></span>] with time-series Sentinel-2 imagery. Seasonal composites of Red, Green, and Blue bands were generated at different phenological stages to support segmentation. The method was applied across over 32 million hectares (79 million acres) of cultivated land in the Canadian Prairies, achieving an intersection-over-union (IoU) accuracy of 0.86 compared to manual segmentation. The workflow consists of four main steps: (1) setting the python working environment, (2) seasonal image acquisition and preprocessing using Google Earth Engine via Python API; (3) field boundary segmentation using SAM; and (4) post-processing and feature cleaning using ArcGIS Pro. This approach demonstrates a scalable, efficient solution for large-scale field boundary mapping to support PA applications.<ul><li><span>•</span><span><div>Integrates a foundation segmentation model (SAM) with Sentinel-2 seasonal imagery</div></span></li><li><span>•</span><span><div>Demonstrates high-accuracy, large-scale automated field boundary delineation</div></span></li><li><span>•</span><span><div>Provides a reproducible workflow adaptable to other regions and datasets</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103794"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2026-06-01Epub Date: 2025-12-08DOI: 10.1016/j.mex.2025.103752
Akitoshi Seiyama , Fengcheng Wei , Masato Ohmi
{"title":"An approach to in vivo low-magnification optical coherence tomography for selective monitoring of tissue glucose concentration","authors":"Akitoshi Seiyama , Fengcheng Wei , Masato Ohmi","doi":"10.1016/j.mex.2025.103752","DOIUrl":"10.1016/j.mex.2025.103752","url":null,"abstract":"<div><div>The low-magnification optical coherence tomography (LM-OCT) is expected to enable the quantification of tissue glucose concentration (TGC) by maintaining high spatial resolution while reducing artifacts caused by tissue heterogeneity. In the present study, we report the practical application of LM-OCT using a conventional OCT system, i.e., without compromising spatial resolution, for the selective monitoring of changes in TGC through <em>in vivo</em> experiments using a hairless rat model.</div><div>A commercially available high-spatial resolution OCT device with a center wavelength of 1300 nm was used to detect the three-dimensional (3D) skin surface structure and the scattering coefficient related to the OCT slope.</div><div>The grand-averaged OCT signal was employed to estimate the OCT slope, while the original high-resolution OCT signals were used to reconstruct the 3D-structural image.</div><div>A strong linear correlation was observed between changes in the OCT slope and that in TGC, but not with 2-deoxy-glucose, lactate, or Intralipos (soybean oil).</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103752"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimizing blue poo: A validated, cost-effective method for measuring whole gut transit time","authors":"Cyra Schmandt , Julia Trunz , Claudio Perret , Anneke Hertig-Godeschalk , Zeno Stanga , Jivko Stoyanov","doi":"10.1016/j.mex.2025.103741","DOIUrl":"10.1016/j.mex.2025.103741","url":null,"abstract":"<div><div>Whole gut transit time (WGTT) provides essential insights into gastrointestinal health, but traditional measurement methods are often expensive or invasive. This study optimizes and validates the \"blue dye method,\" an affordable and minimally invasive approach to WGTT measurement. Using \"Hollinger Farbpulver Blau\" (containing food colors E131 and E132), dye concentrations ranging from 30 mg to 241 mg were tested across four modes of delivery: capsule with liquid, gummy bear, muffin, and capsule with rice crackers and liquid. Each presented limitations: capsules taken with liquid led to inconsistent transit times, gummy bears caused staining, and muffins were perishable. Measured WGTTs varied between 18 and 29 h depending on the mode of delivery and dye concentration. Optimal protocol was a capsule containing 60 mg of dye taken with two rice crackers and liquid, ensuring accurate detection without practical inconveniences. The standardized and optimized blue dye method provides valid WGTT measurements, making it well suited for large-scale population studies and clinical applications.</div><div>Uses a simple blue dye as a marker for gut transit.</div><div>Tested several modes of delivery and concentrations to find the most practical option.</div><div>Established a standardized protocol for reliable and reproducible measurement.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103741"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145692906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Systematic review of gabion-faced geogrid and pile systems for slope and embankment stability","authors":"Devi Oktaviana Latif, Virananda Samudera Rahmadhian, Amalia Ula Hazhiyah","doi":"10.1016/j.mex.2025.103767","DOIUrl":"10.1016/j.mex.2025.103767","url":null,"abstract":"<div><div>Slope instability is a major geotechnical hazard intensified by rainfall infiltration, seismic loading, groundwater fluctuations, and human disturbances. Composite reinforcement systems—such as gabion-faced geogrid walls combined with piles or geosynthetic-encased columns (GECs)—are increasingly implemented to address multi-hazard conditions. This study presents a PRISMA-guided systematic review of empirical, numerical, centrifuge, and field investigations on hybrid slope-stabilization systems. The review advances prior work by explicitly incorporating multi-field coupling and soil–structure interaction (SSI) terms into the search strategy, applying transparent screening and data-extraction procedures supported by a reusable metadata codebook, and conducting cross-study triangulation across field evidence, centrifuge modelling, and 2D/3D numerical analyses. The synthesized evidence shows that hybrid systems can significantly enhance slope performance, with reported improvements of up to ∼45 % in factor of safety and >30 % reduction in settlement, depending on reinforcement configuration, soil conditions, and coupled rainfall–seismic effects. The study further highlights current limitations in optimisation practice, long-term monitoring, and design standardisation, and outlines directions for uncertainty-aware and performance-based slope design.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103767"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2026-06-01Epub Date: 2026-01-16DOI: 10.1016/j.mex.2026.103800
Letícia da Silva Brito , Sidinei Magela Thomaz , Heliana Teixeira , Ana I. Lillebø
{"title":"Ex-situ growth protocol for the invasive macrophyte Pontederia crassipes","authors":"Letícia da Silva Brito , Sidinei Magela Thomaz , Heliana Teixeira , Ana I. Lillebø","doi":"10.1016/j.mex.2026.103800","DOIUrl":"10.1016/j.mex.2026.103800","url":null,"abstract":"<div><div><em>Pontederia crassipes</em> is known for its asexual reproduction and rapid growth. Outside its native range, it has been identified as an environmental threat, while it has also been widely used for <em>ex-situ</em> phytoremediation. To understand both its invasive potential and its phytoremediation capacity, it is necessary to examine the environmental factors that favor its growth beyond those already described in the literature, such as water temperature and nutrient availability. Previous studies also suggest that alkalinity, conductivity, dissolved oxygen, salinity, water depth and pH. These variables help define the species niche and highlight the importance of distinguishing between its fundamental niche, the full set of abiotic conditions that support growth and its realized niche, which reflects biotic interactions and local constraints. However, the scientific literature does not yet provide sufficient description of the <em>ex-situ</em> experimental conditions required for the successful cultivation of this aquatic plant in controlled settings. This protocol therefore reports the results and lessons learned from a series of mesocosm experiments. By standardizing procedures and documenting growth outcomes, the protocol enhances reproducibility, facilitates comparisons across studies and supports both basic and applied research on <em>P. crassipes.</em></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103800"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2026-06-01Epub Date: 2026-01-08DOI: 10.1016/j.mex.2026.103786
Marziya Begum, Akaitab Mukherjee
{"title":"The neurocultural remake reflex model: An interdisciplinary tool for understanding film remakes","authors":"Marziya Begum, Akaitab Mukherjee","doi":"10.1016/j.mex.2026.103786","DOIUrl":"10.1016/j.mex.2026.103786","url":null,"abstract":"<div><div>The <strong>Neurocultural Remake Reflex Model (NRRM)</strong> is an interdisciplinary qualitative and neuroscience-informed analytical method that integrates heuristic insights from intersubject correlation (ISC) literature, cultural memory theory and affect theory to make reception-centered, testable inferences about how remakes trigger recognition, emotion and reinterpretation. The six-steps involved in the framework, <strong>Attention Mapping, Cognitive Processing, Cultural Recall, Cultural Reframing, Emotional Resonance and Emotional Dissonance</strong>are operationalized through systematic qualitative scene analysis rather than empirical neuroimaging<strong>.</strong> Most existing research on remakes has primarily focused on comparative textual analysis, often overlooking the neurocognitive and emotional aspects of audience reception. The NRRM addresses this gap by explaining how viewers attend to, interpret, remember, emotionally connect with, and sometimes resist or contest a remake’s narrative and aesthetic cues. The paper demonstrates the method through a scene-level application of two case studies to show how remakes function simultaneously as mnemonic devices and affective tools. In doing so, NRRM reveals how remakes preserve cultural legacies while generating new meanings, offering film scholars, media psychologists and cultural analysts a reproducible framework for tracing how remakes conserve, reframe or disrupt cultural memory and affective experience.</div><div>This study proposes a six-step systematic qualitative methodology for analyzing remakes.</div><div>By integrating cognitive and cultural approaches, it introduces an interdisciplinary framework for remake analysis.</div><div>This framework demonstrates how remakes function as neurocultural reflexes, triggering nostalgia through both resonance and disruption.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103786"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MethodsXPub Date : 2026-06-01Epub Date: 2025-12-06DOI: 10.1016/j.mex.2025.103749
Jada Tarvin-Imeokparia , Adeyemi Abejoye , Isabella Vega , Max Abel Alphonse Mendoza , Campbell Moses , Myalia Durno , Meghan S. Cahill , Christian O. Dimkpa , Jason C. White , Shelley Durocher , Frederick Pettit , Mark Meyering , Jonathan M. Jacobs , Maria Soledad Benitez Ponce , Nathaniel Heiden
{"title":"Application of multiple parallel mineralization method to grow lettuce, Swiss chard, and peppers in a simplified deep flow technique hydroponic system","authors":"Jada Tarvin-Imeokparia , Adeyemi Abejoye , Isabella Vega , Max Abel Alphonse Mendoza , Campbell Moses , Myalia Durno , Meghan S. Cahill , Christian O. Dimkpa , Jason C. White , Shelley Durocher , Frederick Pettit , Mark Meyering , Jonathan M. Jacobs , Maria Soledad Benitez Ponce , Nathaniel Heiden","doi":"10.1016/j.mex.2025.103749","DOIUrl":"10.1016/j.mex.2025.103749","url":null,"abstract":"<div><div>Organic fertilizers are not widely used in hydroponics due to an absence of mineralizing microbes that process organic inputs into plant available forms. The Multiple Parallel Mineralization (MPM) method enables this microbial processing to occur in hydroponic systems. The objective of this method is to provide an accessible protocol for use of the MPM method in simplified deep flow technique hydroponic systems without a need for laboratory testing of nutrient content. The method described here provides hydroponic growers with an alternative option to mineral fertilizers. We provide information about yields and the affordability of this method that growers can use to determine the economic viability of this method in their respective markets.</div><div>Swiss chard, lettuce, and bell peppers were grown with this method</div><div>Aquarium nitrate test kits are used to replace lab testing typically used with the MPM method to validate the presence of nitrate as an indicator of microbial activity</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103749"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}