Lipids in Health and Disease最新文献

{"title":"Multiomics analysis revealed the regulatory role of chenodeoxycholic acid in fatty acid metabolism and lipid homeostasis.","authors":"Lishuang Li, Xing Ren, Xinyu Gao, Yujia Zhao, Yuman Ma, Junxiao Gong, Shuo Yang, Yanan Sun, Yi Wang","doi":"10.1186/s12944-025-02656-w","DOIUrl":"10.1186/s12944-025-02656-w","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the regulatory role and mechanisms of chenodeoxycholic acid (CDCA) on lipid metabolism, and to evaluate its therapeutic potential in lipid metabolism-related diseases such as acne, seborrheic alopecia, and prostate cancer, with the aim of identifying safer and more sustainable treatment alternatives.</p><p><strong>Methods: </strong>Lipid-overproduction models were established using RM-1 (murine prostate cancer cells) and primary sebaceous gland cells, stimulated by linoleic acid (LA) and dihydrotestosterone (DHT), respectively. CDCA's mechanisms were explored using transcriptomics, proteomics, and fatty acid-targeted metabolomics. In vivo validation was conducted via intradermal injection of CDCA into the sebaceous gland area of golden hamsters to assess its effect on sebaceous lipid metabolism.</p><p><strong>Results: </strong>CDCA significantly reduced intracellular lipid accumulation in both murine prostate cancer cells (RM-1) and primary sebaceous gland cells, and suppressed the expression of the androgen receptor (AR), along with the downregulation of key lipogenic enzymes (SREBF1, FASN, FADS2). Mitochondrial membrane potential was restored in CDCA-treated cells. Multi-omics analyses revealed that CDCA modulated fatty acid biosynthesis and degradation, energy metabolism, mitochondrial function, and Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ) signaling. Lipidomic profiling demonstrated a shift from saturated to unsaturated fatty acid dominance after CDCA treatment. In vivo, CDCA decreased sebaceous lipid accumulation and downregulated PPAR-γ expression in golden hamsters.</p><p><strong>Conclusion: </strong>CDCA exerted multifaceted regulatory effects on lipid metabolism, hormone signaling, and mitochondrial dynamics. These effects contributed to the maintenance of sebaceous gland homeostasis and supported the development of innovative and potentially more biocompatible therapies for lipid-related disorders.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"238"},"PeriodicalIF":3.9,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein, fiber, and exercise: a narrative review of their roles in weight management and cardiometabolic health.","authors":"Liana L Guarneiri, Carol F Kirkpatrick, Kevin C Maki","doi":"10.1186/s12944-025-02659-7","DOIUrl":"10.1186/s12944-025-02659-7","url":null,"abstract":"<p><p>More than 40% of adults in the United States have obesity, which is an independent risk factor for cardiometabolic dysfunction and several chronic diseases, including atherosclerotic cardiovascular disease and type 2 diabetes mellitus. Interventions for obesity include lifestyle modification, pharmacotherapy, and metabolic surgery. Lifestyle interventions have minimal side effects and are the most economical, yet long-term adherence is poor. Challenges for long-term adherence are related, in part, to metabolic adaptations that occur during weight loss that affect appetite and energy expenditure. However, a lifestyle intervention that involves higher intakes of protein and fiber and increased participation in exercise may help to blunt the increases in hunger and cravings that occur with weight loss while increasing energy expenditure, thus assisting with both weight loss and weight loss maintenance. These changes also promote improved cardiometabolic health, independent of weight loss. This narrative review summarizes the evidence for the roles of increasing protein, fiber, and exercise for weight management and improving the cardiometabolic risk factor profile.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"237"},"PeriodicalIF":3.9,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linking metabolic score for visceral fat to heart failure: insights from national health and nutrition examination survey 2003-2018.","authors":"Daoliang Zhang, Wenrui Shi, Jiakun Liu, Younan Yao, Jian Zhang","doi":"10.1186/s12944-025-02654-y","DOIUrl":"10.1186/s12944-025-02654-y","url":null,"abstract":"<p><strong>Background: </strong>Visceral fat has become increasingly recognized as a key modifiable risk factor in the development of heart failure (HF). This study aims to investigate the link between the Metabolic Score for Visceral Fat (METS-VF) and the prevalence of HF, and evaluate whether METS-VF could improve the detection of HF in the general population.</p><p><strong>Methods: </strong>24,681 subjects from the 2003-2018 National Health and Nutrition Examination Survey were included in the analysis. HF was determined according to self-reported medical history.</p><p><strong>Results: </strong>The overall prevalence of HF was found to be 2.91%. After adjusting for relevant confounders, each one-standard-deviation increase in METS-VF was associated with a 75.4% higher risk of prevalent HF. Participants with METS-VF values in the highest quartile had a 2.731-fold greater risk of prevalent HF than those in the lowest quartile. The relationship between METS-VF and HF appeared nearly linear based on smooth-curve modeling, and subgroup analyses confirmed this association across different demographic groups. When METS-VF was added to standard cardiovascular risk factors, there was a slight improvement in HF discrimination (AUC: 0.867 vs. 0.873, P = 0.003). Reclassification metrics also highlighted the incremental value of including METS-VF.</p><p><strong>Conclusion: </strong>The findings of this study demonstrated a strong, linear relationship between METS-VF and the prevalence of HF, suggesting that METS-VF could be an adjunctive tool for improving HF screening in the general population.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"235"},"PeriodicalIF":3.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NSUN2 knockdown ameliorates hepatic glucose and lipid metabolism disorders in type 2 diabetes mellitus through the Inhibition of ACSL6 m5C methylation.","authors":"Xianshu Jiang, Xuelan Cheng, Qiu Wan","doi":"10.1186/s12944-025-02652-0","DOIUrl":"10.1186/s12944-025-02652-0","url":null,"abstract":"<p><strong>Background: </strong>Hyperglycemia and dyslipidemia characterize type 2 diabetes mellitus (T2DM). The impact of 5-methylcytosine (m5C) on liver glucose and lipid regulation in T2DM is unclear. In this study, the regulation of liver glucose and lipid metabolism by m5C methylation in a T2DM model was investigated.</p><p><strong>Methods: </strong>C57BL/6 mice developed T2DM via a high-fat diet (HFD) and streptozotocin (STZ) administration. Quantitative real-time PCR was performed to assess m5C-associated gene expression in HFD-fed mice and NSUN2 levels in clinical samples. Glucose metabolism was assessed via glucose metabolism function tests. Lipid metabolic parameters were evaluated through pathological analysis and measurements of biochemical indicators in HFD-fed mice. Mechanistic investigations utilized methylated RNA immunoprecipitation (MeRIP), RNA immunoprecipitation (RIP) and RIP.</p><p><strong>Results: </strong>NSUN2 expression was significantly upregulated in both T2DM clinical samples and HFD-fed mice. NSUN2 knockdown enhanced glucose tolerance and pyruvate metabolism, ameliorated insulin resistance, and suppressed hepatic lipid accumulation in HFD-fed mice. Mechanistically, NSUN2 depletion reduced ACSL6 expression by inhibiting m5C modification of ACSL6 mRNA. Furthermore, the metabolic improvements in HFD-fed mice following NSUN2 knockdown were partially reversed by ACSL6 overexpression.</p><p><strong>Conclusion: </strong>This study demonstrated that NSUN2 mediates glucose and lipid metabolism dysregulation in the liver during T2DM through m5C-dependent ACSL6. These findings highlight NSUN2 as a viable target for innovative T2DM treatments.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"236"},"PeriodicalIF":3.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of an LDH-focused nomogram for the early prediction of heart failure in patients with acute ST-elevation myocardial infarction after percutaneous coronary intervention.","authors":"Panting Wei, Jingjin Hou, Ruijiao Yu, Kun Na, Jiaqi He, Xiaojie Zhao, Ziqi Liu, Yan Xu, Yi Li, Chenghui Yan, Haibo Yu, Yaling Han","doi":"10.1186/s12944-025-02649-9","DOIUrl":"10.1186/s12944-025-02649-9","url":null,"abstract":"<p><strong>Background: </strong>ST-elevation myocardial infarction (STEMI) patients remain at significant risk of heart failure (HF) despite successful percutaneous coronary intervention (PCI) reperfusion, imposing a considerable public health burden. Lactate dehydrogenase (LDH), a key enzyme in glycolysis, functions as a notable biomarker of cardiac pathology, yet it is frequently often overlooked in clinical practice. This study focused on establishing a nomogram incorporating LDH levels to assess the probability of HF occurring within one year following PCI in individuals who have previously experienced STEMI.</p><p><strong>Methods: </strong>527 patients diagnosed with STEMI were initially included in this study. After excluding 71 patients with ejection fractions < 40% and other cardiovascular diseases, the final cohort of 456 patients with STEMI was recruited and randomly assigned to the training and validation sets. To identify potential risk factors linked to HF, both univariate and multivariate logistic regression analyses were conducted, leading to the development of a predictive nomogram. Model performance in terms of discrimination, calibration, and clinical usefulness was validated using receiver operating characteristic (ROC) curves, calibration plots, and decision curve analyses (DCA).</p><p><strong>Results: </strong>Patients with STEMI who developed HF within one year exhibited higher LDH levels within 24 hours post-PCI. A nomogram model was constructed that included alcohol drinking, left anterior descending artery involvement, and creatine kinase-MB, log N-terminal pro b-type natriuretic peptide, and LDH levels. The area under the ROC curve (AUC) was 0.831 (95% CI: 0.7807-0.8805), and the DCA demonstrated that the model offered a considerable net benefit when the threshold probability varied between 1% and 97% within the training dataset. Additionally, LDH demonstrated excellent predictive capability for HF, with an AUC of 0.756 (95% CI: 0.706-0.806). It showed even better performance for HF with a reduced ejection fraction, with an AUC of 0.848 (95% CI: 0.7705-0.9249).</p><p><strong>Conclusions: </strong>LDH independently predicts the development of HF within one year of PCI in patients with STEMI. The LDH-based nomogram demonstrated a robust predictive capability. It enables early identification and timely intervention in STEMI patients at an elevated risk of HF, which is crucial for reducing HF incidence and alleviating the associated healthcare burden.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"233"},"PeriodicalIF":3.9,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of estimated glucose disposal rate and high sensitivity C-reactive protein on risk of incident cardiovascular diseases in middle-aged and elderly Chinese adults: a nationwide prospective cohort study.","authors":"Linghao Xu, Jiangping Ma, Yong Xu","doi":"10.1186/s12944-025-02653-z","DOIUrl":"10.1186/s12944-025-02653-z","url":null,"abstract":"<p><strong>Aim: </strong>Cardiovascular diseases (CVD) remain the predominant contributor to global mortality and morbidity, necessitating enhanced strategies for primary prevention and early detection. This investigation sought to characterize the associations between estimated glucose disposal rate (eGDR), high sensitivity C-reactive protein (hsCRP), and incident CVD.</p><p><strong>Methods: </strong>The research used data from China Health and Retirement Longitudinal Study (CHARLS). The primary exposures included eGDR and hsCRP. Incident CVD served as the primary study endpoint. All statistical analyses were performed utilizing SPSS software (version 25.0) and R software (version 4.4.4).</p><p><strong>Results: </strong>The analysis of 7,742 participants revealed incident CVD in 1,981 cases (25.6%), with Kaplan-Meier curves demonstrating significantly elevated cumulative incidence among individuals exhibiting both reduced eGDR and elevated hsCRP levels. Significant interactions were observed between these biomarkers, including an additive effect (relative excess risk due to interaction = 0.09, 95% confidence interval [CI]: 0.03-0.14) and a multiplicative effect (hazard ratio = 1.02, 95% CI: 1.01-1.03). Mediation analyses indicated bidirectional relationships, with eGDR accounting for 5.81% (95% CI: 2.13-9.49%) of the hsCRP-CVD association and hsCRP mediating 8.52% (95% CI: 3.87-13.17%) of the eGDR-CVD relationship. Notably, in individuals with diabetes, the combined predictive capacity of eGDR and hsCRP (area under the curve: 0.645) demonstrated superior discriminative performance compared to either biomarker alone (AUC: 0.645).</p><p><strong>Conclusion: </strong>eGDR and hsCRP exert significant joint, interactive and mediating effects on incident CVD in middle-aged and elderly Chinese population. Using eGDR (10.52 mg/kg/min) and hsCRP (1 mg/L) thresholds can be an affordable screening tool in primary care, especially where advanced diagnostics are lacking. Incorporating these assessments into regular check-ups may lower long-term CVD complications and reduce economic burdens.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"234"},"PeriodicalIF":3.9,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of dyslipidemia and its association with blood pressure control in Iranian hypertensive patients: insights from STEPS 2021.","authors":"Shervin Mossavarali, Yosra Azizpour, Ali Golestani, Nazila Rezaei, Sepehr Khosravi, Mina Mirzad, Pouria Khashayar, Ozra Tabatabaei-Malazy","doi":"10.1186/s12944-025-02660-0","DOIUrl":"10.1186/s12944-025-02660-0","url":null,"abstract":"<p><strong>Background: </strong>Given the association between dyslipidemia and hypertension and their significant impact on cardiovascular diseases (CVDs) in Middle Eastern countries, coordinated monitoring of these risk factors across the region is essential. This study aimed to assess the prevalence of dyslipidemia among hypertensive Iranians and explore its association with hypertension control.</p><p><strong>Methods: </strong>Data from the Iranian STEPwise Approach to Non-communicable Disease (NCD) Risk Factor Surveillance (STEPS) 2021 survey, including questionnaires, physical measurements, laboratory tests, from a total of 5,997 participants, were analyzed. Hypertension was defined as the current use of antihypertensive medication or blood pressure ≥ 140/90 mmHg. Lipid markers, including total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), Non-HDL-C, and triglycerides (TG), were evaluated using the National Cholesterol Education Program Adult Treatment Panel III 2004 criteria. Associations between lipid markers and uncontrolled hypertension were assessed using a multivariable modified Poisson regression model, with results presented as adjusted prevalence ratio (APR) and 95% confidence interval (CI). All analyses were conducted utilizing STATA software version 14.2.</p><p><strong>Results: </strong>The prevalence of dyslipidemia among hypertensive Iranian adults was 86.4% [95% CI: 85.0, 87.6], with the most common abnormalities being low HDL-C 71.3% [95% CI: 69.6, 72.9], followed by hypertriglyceridemia 48.4% [95% CI: 46.5, 50.2], hypercholesterolemia 24.9% [95% CI: 23.3, 26.6], and high LDL-C 18.8% [95% CI: 17.3, 20.3]. In the multivariable model, TC, LDL-C, Non-HDL-C, TC/HDL-C, and LDL-C/HDL-C were significantly associated with uncontrolled hypertension in both males and females. Each 1 mmol/L increase in LDL-C was associated with a higher prevalence of uncontrolled hypertension by 1.07 [95% CI: 1.04, 1.09] in males and 1.05 [95% CI: 1.03, 1.07] in females; Non-HDL-C by 1.06 [95% CI: 1.04, 1.08] in males and 1.05 [95% CI: 1.03, 1.07] in females; TC by 1.06 [95% CI: 1.04, 1.08] in males and 1.05 [95% CI: 1.03, 1.07] in females; and TG by 1.02 [95% CI: 1.00, 1.03] in males.</p><p><strong>Conclusions: </strong>Dyslipidemia is highly prevalent among hypertensive Iranian adults and may be associated with poor blood pressure control. In particular, low HDL-C emerged as the most frequent lipid abnormality and should be carefully assessed and managed as part of integrated, preventive hypertension care.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"232"},"PeriodicalIF":3.9,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C-reactive protein as a mediator in the link between cardiometabolic index and osteoarthritis: insights from NHANES 2001-2010.","authors":"Yike Wang, Zeyu Liu, Haoyu Wu, Chunsheng Wang","doi":"10.1186/s12944-025-02603-9","DOIUrl":"10.1186/s12944-025-02603-9","url":null,"abstract":"<p><strong>Background: </strong>Evidence has noted associations of osteoarthritis (OA) with obesity and C-reactive protein (CRP). However, little is clarified about the link between cardiometabolic index (CMI) and OA and the mediating role of CRP.</p><p><strong>Methods: </strong>Participants from NHANES 2001-2010 were enrolled. CMI was computed based on anthropometric and biochemical indexes, covering height, waist circumference, triglyceride, and high-density lipoprotein cholesterol. OA diagnosis data were derived from the Medical Conditions questionnaires. Multivariate logistic regressions, restricted cubic spline (RCS) analysis, subgroup analyses, and interaction tests were implemented to investigate the association. A mediation analysis was employed to verify the mediating role of CRP.</p><p><strong>Results: </strong>After controlling for all covariates, CMI was significantly linked with OA (OR: 1.07, 95% CI (1.01, 1.13), P = 0.038). RCS analyses elicited a non-linear link between CMI and OA (P - non - linear = 0.0012). The inflection point was 0.54. Threshold effect analysis was conducted through two-segment linear regression, including the left side ( < = 0.54) (OR: 2.06, 95% CI: 1.03, 6.58) and the right side of the inflection point (> 0.54) (OR: 0.40, 95% CI: 0.15, 1.03). CRP partially mediated the link between CMI and OA, around 7.59% of the total effect.</p><p><strong>Conclusion: </strong>CMI is positively linked with OA, with CRP mediating the link. This paper unveils novel perspectives on the mechanism that links CMI to OA. Managing CMI and monitoring CRP levels may alleviate OA.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"231"},"PeriodicalIF":3.9,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PPP2CB aggravates atherosclerosis-related dyslipidemia via LOX-1/MAPK/ERK signaling pathway.","authors":"He An, Dong-Liang Cheng, Xian-Ru Xia, Xian-Dong Li, Zhi-Hua Ruan, Chun-Yan Peng","doi":"10.1186/s12944-025-02647-x","DOIUrl":"10.1186/s12944-025-02647-x","url":null,"abstract":"<p><strong>Background: </strong>Dyslipidemia has been extensively documented as a key driver of cardiovascular pathology. Regulating lipid homeostasis holds promise for treating atherosclerosis (AS). Although the protein phosphatase 2 catalytic subunit beta (PPP2CB) is involved in post-transcriptional gene regulation, its role in AS-associated dyslipidemia is not well understood.</p><p><strong>Methods: </strong>The study included both human participants and animal models. The following techniques were employed: cell culture, extraction of exosomes, preparation of pooled hyperlipidemic serum (HS), transfection, western blotting, immunofluorescence staining, quantitative reverse transcription polymerase chain reaction (qRT-PCR), co-immunoprecipitation, low-density lipoprotein cholesterol (LDL-C) uptake assay, biochemical assays, assessment of aortic atherosclerotic lesions, as well as statistical analysis.</p><p><strong>Results: </strong>This study identified a marked upregulation of PPP2CB expression in peripheral blood leukocytes of AS patients, artery plaque of ApoE^-/- mice given a high-fat diet, and hepatic cells exposed to hyperlipidemic stimuli. Overexpression of PPP2CB in hepatic cells exacerbated lipid accumulation and low-density lipoprotein uptake, whereas silencing PPP2CB mitigated this effect. Immunofluorescence co-localization and co-immunoprecipitation analysis confirmed a direct interaction between PPP2CB and lectin-like oxidized LDL receptor-1 (LOX-1). Notably, PPP2CB manipulation disrupted hyperlipidemia-induced LOX-1 expression. Additionally, PPP2CB-mediated lipid dysregulation was linked to the activation of the LOX-1/ mitogen-activated protein kinase (MAPK)/ extracellular signal-regulated kinase (ERK) signaling cascade.</p><p><strong>Conclusions: </strong>These results unveil PPP2CB as a novel lipid regulator in the progression of pathological AS and highlight its involvement in signaling regulation during abnormal lipid metabolism. PPP2CB could be considered a promising candidate for biomarker development and therapeutic intervention in AS.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"229"},"PeriodicalIF":3.9,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The value of triglyceride-glucose index-related indices in evaluating migraine: perspectives from multi-centre cross-sectional studies and machine learning models.","authors":"Zixuan Yan, Lincheng Duan, Hong Yin, Muchen Wang, Jingwen Li, Chenghua Li, Xiao Wang, Dingjun Cai, Fanrong Liang, Wenchuan Qi","doi":"10.1186/s12944-025-02648-w","DOIUrl":"10.1186/s12944-025-02648-w","url":null,"abstract":"<p><strong>Background: </strong>This study employed representative data from the U.S. and China to delve into the correlation among migraine prevalence, the triglyceride‒glucose index, a marker of insulin resistance, and the composite indicator of obesity.</p><p><strong>Methods: </strong>Cross-sectional data were acquired from the National Health and Nutrition Examination Survey conducted between 1999 and 2004, as well as from the China Longitudinal Study of Health and Retirement (CHARLS) performed from 2011 to 2012. Weighted logistic regression analysis, subgroup analysis, smooth curve fitting and threshold effect analysis were used to ascertain the intricate relationships among triglyceride glucose-body mass index (TyG-BMI), triglyceride glucose-waist circumference (TyG-WC), triglyceride glucose-waist height ratio (TyG-WHtR) and migraine. Boruta's algorithm and nine machine learning models were applied. SHapley Additive Explanations (SHAP) values were used to analyze leading models, highlighting influential features.</p><p><strong>Results: </strong>The analysis included 6,204 U.S. participants and 9,401 Chinese participants. TyG-BMI as well as TyG-WHtR were shown to be strongly correlated with the incidence of migraine among U.S. adults (TyG-BMI: OR = 1.28, 95% CI 1.14-1.44, P < 0.001; TyG-WHtR: OR = 1.17, 95% CI 1.09-1.26, P < 0.001). However, this correlation was not detected in Chinese adults. TyG-BMI indicated a strong positive association beyond the threshold of 206, while TyG-WHtR demonstrated a significant positive link below the cutoff of 7.4. In addition, age was an important interaction factor between TyG-BMI and TyG-WHtR and migraine. The XGBoost model showed excellent performance, with higher AUC values for TyG-BMI than for TyG-WHtR (0.929/0.926).</p><p><strong>Conclusions: </strong>The TyG-BMI, relative to the TyG-WHtR, may provide clinicians with information about patients' insulin sensitivity, thus helping to develop individualized treatment strategies. These findings contribute to population-level health interventions aimed at mitigating metabolic and neurological disease burdens, ensuring healthy lives and promoting well-being.</p>","PeriodicalId":18073,"journal":{"name":"Lipids in Health and Disease","volume":"24 1","pages":"230"},"PeriodicalIF":3.9,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}