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Differential effects of anesthetics and sex on supraventricular electrophysiology and atrial fibrillation substrate in rats 麻醉药和性别对大鼠室上电生理和房颤底物的差异影响
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-26 DOI: 10.1038/s41684-025-01532-5
Michael Murninkas, Or Levi, Sigal Elyagon, Aviv Komissar, Neta Marom, Alon Naumchik, Noam Dalal, Gideon Gradwohl, Yoram Etzion
{"title":"Differential effects of anesthetics and sex on supraventricular electrophysiology and atrial fibrillation substrate in rats","authors":"Michael Murninkas, Or Levi, Sigal Elyagon, Aviv Komissar, Neta Marom, Alon Naumchik, Noam Dalal, Gideon Gradwohl, Yoram Etzion","doi":"10.1038/s41684-025-01532-5","DOIUrl":"10.1038/s41684-025-01532-5","url":null,"abstract":"Rodents are increasingly used in atrial electrophysiology research, yet such studies are often performed under anesthesia owing to technical challenges. Here we developed an implantable device for comprehensive atrial studies in ambulatory rats and investigated the effects of commonly used anesthetics on supraventricular electrophysiology and arrhythmic substrate, comparing them with the unanesthetized state (UAS). Adult rats were evaluated 4 weeks after implantation. Studies were conducted in the UAS under 2% isoflurane (ISO) and under 40 mg/kg pentobarbital (PEN). Pacing protocols determined various parameters, including sinoatrial node recovery time, atrioventricular node effective refractory period and atrial effective refractory period. Arrhythmic substrate was assessed after 20 triggering bursts per condition, and arrhythmic tendency was analyzed manually and through the complexity ratio, an unbiased measure recently developed by our group. PEN mildly increased heart rate in both sexes, while ISO did not affect heart rate but prolonged the corrected sinus node recovery time in males. PEN increased atrioventricular node effective refractory period in both sexes, while ISO affected males only. Both ISO and PEN prolonged atrial effective refractory period compared with UAS in both sexes. Arrhythmic measures were higher in males and were attenuated by ISO and, to a lesser extent, by PEN in males only. The dominant frequency of arrhythmic events was reduced by both anesthetics in both sexes. These findings demonstrate a significant impact of commonly used anesthetics on rat supraventricular electrophysiology, with sex-based differences, highlighting the importance of methodologies that enable cardiac electrophysiology studies in unanesthetized rodents. The authors used an implantable electrophysiological device to assess the effects of two commonly used anesthetics (isoflurane and pentobarbital) on supraventricular electrophysiology and arrhythmic substrate in male and female rats.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 4","pages":"80-91"},"PeriodicalIF":5.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-025-01532-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of dietary protein are sex-dependent 膳食蛋白质的影响是性别依赖的
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01525-4
Jorge Ferreira
{"title":"Effects of dietary protein are sex-dependent","authors":"Jorge Ferreira","doi":"10.1038/s41684-025-01525-4","DOIUrl":"10.1038/s41684-025-01525-4","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 3","pages":"60-60"},"PeriodicalIF":5.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new mouse model with human-like telomeres 一种具有类似人类端粒的新小鼠模型
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01530-7
Alexandra Le Bras
{"title":"A new mouse model with human-like telomeres","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01530-7","DOIUrl":"10.1038/s41684-025-01530-7","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 3","pages":"63-63"},"PeriodicalIF":5.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-025-01530-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
NMDA receptors are involved in social facilitation of feeding behavior NMDA受体参与进食行为的社会促进
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01531-6
Jorge Ferreira
{"title":"NMDA receptors are involved in social facilitation of feeding behavior","authors":"Jorge Ferreira","doi":"10.1038/s41684-025-01531-6","DOIUrl":"10.1038/s41684-025-01531-6","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 3","pages":"64-64"},"PeriodicalIF":5.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-025-01531-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pig model to study COVID-19 disease 研究 COVID-19 疾病的猪模型
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01521-8
Alexandra Le Bras
{"title":"Pig model to study COVID-19 disease","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01521-8","DOIUrl":"10.1038/s41684-025-01521-8","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 3","pages":"59-59"},"PeriodicalIF":5.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dimethoate affects rat biochemical parameters 乐果对大鼠生化指标的影响
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01526-3
Jorge Ferreira
{"title":"Dimethoate affects rat biochemical parameters","authors":"Jorge Ferreira","doi":"10.1038/s41684-025-01526-3","DOIUrl":"10.1038/s41684-025-01526-3","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 3","pages":"60-60"},"PeriodicalIF":5.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hydrogen sulfide alleviates chronic pain 硫化氢可减轻慢性疼痛
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01527-2
Jorge Ferreira
{"title":"Hydrogen sulfide alleviates chronic pain","authors":"Jorge Ferreira","doi":"10.1038/s41684-025-01527-2","DOIUrl":"10.1038/s41684-025-01527-2","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 3","pages":"60-60"},"PeriodicalIF":5.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mechanistic insights on sickle cell disease 镰状细胞病的机制见解
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01528-1
Jorge Ferreira
{"title":"Mechanistic insights on sickle cell disease","authors":"Jorge Ferreira","doi":"10.1038/s41684-025-01528-1","DOIUrl":"10.1038/s41684-025-01528-1","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 3","pages":"60-60"},"PeriodicalIF":5.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Age-dependent effects of intermittent fasting in mice 小鼠间歇性禁食的年龄依赖性效应
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01524-5
Alexandra Le Bras
{"title":"Age-dependent effects of intermittent fasting in mice","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01524-5","DOIUrl":"10.1038/s41684-025-01524-5","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 3","pages":"59-59"},"PeriodicalIF":5.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of methylene blue on zebrafish 亚甲基蓝对斑马鱼的影响
IF 5.9 3区 农林科学
Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01522-7
Alexandra Le Bras
{"title":"Effect of methylene blue on zebrafish","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01522-7","DOIUrl":"10.1038/s41684-025-01522-7","url":null,"abstract":"","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 3","pages":"59-59"},"PeriodicalIF":5.9,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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