IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-10-14 DOI: 10.1038/s41684-025-01620-6

Karen Antunes,Nathaniel B Willis,Joseph F Pierre

引用次数: 0

Thermoneutral environment improves mouse welfare and reduces stress in metabolic cages. 热中性环境改善代谢笼小鼠福利，减少应激。

IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-10-10 DOI: 10.1038/s41684-025-01618-0

Philipp Villiger,Charlotte Calvet,Eva Maria Pastor-Arroyo,Carsten A Wagner,Petra Seebeck

{"title":"Thermoneutral environment improves mouse welfare and reduces stress in metabolic cages.","authors":"Philipp Villiger,Charlotte Calvet,Eva Maria Pastor-Arroyo,Carsten A Wagner,Petra Seebeck","doi":"10.1038/s41684-025-01618-0","DOIUrl":"https://doi.org/10.1038/s41684-025-01618-0","url":null,"abstract":"Metabolic cages (MCs) are often used to collect feces and urine samples. However, the housing of mice in MCs can be stressful, potentially affecting parameters of interest. Here we compare our standard protocol for individual MC housing (4 days at 23 °C: 3 days of permanent acclimatization followed by 24 h sampling (MC23)) with a short-term intermittent acclimatization protocol (3 h of MC housing for 3 days plus 24 h sampling (accMC23)), the provision of a nest (4 days at 23 °C in MC (nest-MC23)) and MC housing at thermoneutrality (4 days at 30 °C, MC30)). C57BL6/N mice were implanted with telemetric transmitters to collect electrocardiograms, blood pressure, core body temperature and activity data. Single-housed mice in the MC at 23 °C had lower core body temperatures and higher heart and respiratory rates than mice in the MC30 group. Mice housed in MCs at 23 °C exhibited increased food consumption and weight loss, combined with significantly increased expression of messenger RNAs of key molecules in brown fat compared with mice housed in MCs at 30 °C. They also showed increased corticosterone levels. Some male mice of the MC23 and accMC23 groups exhibited episodes of reduced core body temperature and reduced blood pressure and heart rate. Our study demonstrates that housing mice in MCs at 23 °C has a substantial impact on their physiology and welfare due to a substantial cold stress. MC housing at thermoneutrality (30 °C) provides a simple solution to improve mouse welfare. Furthermore, the results showed that a single acclimatization period had the same effect as repeated exposure to the MCs and, therefore, provided no additional benefit.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"35 1","pages":""},"PeriodicalIF":6.9,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145261272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving pain management for murine orthotopic xenograft models of acute lymphoblastic leukemia. 改善急性淋巴细胞白血病小鼠原位异种移植模型的疼痛管理。

IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-10-08 DOI: 10.1038/s41684-025-01615-3

Tim Schreiber,Sandra Lange,Steven R Talbot,Jakob Brandstetter,Emily Leitner,Christian Junghanss,Brigitte Vollmar,Rupert Palme,Anna Richter,Simone Kumstel

{"title":"Improving pain management for murine orthotopic xenograft models of acute lymphoblastic leukemia.","authors":"Tim Schreiber,Sandra Lange,Steven R Talbot,Jakob Brandstetter,Emily Leitner,Christian Junghanss,Brigitte Vollmar,Rupert Palme,Anna Richter,Simone Kumstel","doi":"10.1038/s41684-025-01615-3","DOIUrl":"https://doi.org/10.1038/s41684-025-01615-3","url":null,"abstract":"Despite ongoing research, realistic in vitro models for acute lymphoblastic leukemia (ALL) that can mimic the complex pathology are still not available, highlighting the need for continuous animal-based investigation. As part of the 3R principles, constant refinement of animal experiments is mandatory. Therefore, reviewing the effectiveness of used analgesics is essential for animal model-specific refinement. Here we evaluate whether metamizole-previously used in our institute-or tramadol is more suitable as on-demand analgesia in mouse models of ALL. The murine orthotopic xenograft models were induced by intravenous injection of either the human precursor ALL cell lines RS4;11 or SEM into immune-deficient male and female NSG mice. Mice were weighed and checked daily for basic behavior and well-being, while detailed welfare parameters, such as burrowing behavior, nesting activity, perianal temperature, liquid intake, fecal corticosterone metabolites, mouse grimace scale and tumor cell proliferation were monitored weekly. Upon leukemic progression, when signs of pain or discomfort were observed, metamizole (3 mg/ml) or tramadol (1 mg/ml) was administered via drinking water for analgesic treatment, and detailed welfare parameters were assessed daily. Following the initiation of treatment, mice receiving either metamizole or tramadol continued to show a decline in body weight, liquid intake and other welfare parameters, suggesting that neither drug was sufficient to fully counteract the effects of late-stage ALL. Combining the data with the relative severity assessment algorithm revealed that metamizole treatment appeared less effective than tramadol in mitigating the detrimental effects of the disease. Therefore, the opioid tramadol should replace metamizole as the analgesic compound of choice for hematological xenograft models to improve animal welfare in future studies.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"89 1","pages":""},"PeriodicalIF":6.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From passive defense to proactive detoxification: transformative strategies in mercury bioremediation. 从被动防御到主动解毒：汞生物修复的变革策略。

IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-10-07 DOI: 10.1038/s41684-025-01632-2

Yan Guo,Chang-Ye Hui

引用次数: 0

Digital embryo 数字胚胎

IF 3.9 3区农林科学

Lab Animal Pub Date : 2025-09-30 DOI: 10.1038/s41684-025-01626-0

Alexandra Le Bras

引用次数: 0

Strain differences in endometriosis models 子宫内膜异位症模型的应变差异