Lab Animal最新文献_第2页

Behavioral profile of mice with Duchenne muscular dystrophy

IF 5.9 3区农林科学

Lab Animal Pub Date : 2025-04-01 DOI: 10.1038/s41684-025-01534-3

Jorge Ferreira

引用次数: 0

Pathway involved in ethanol-induced craniofacial malformations

IF 5.9 3区农林科学

Lab Animal Pub Date : 2025-04-01 DOI: 10.1038/s41684-025-01542-3

Jorge Ferreira

引用次数: 0

Predicting non-recovery stages of sepsis

IF 5.9 3区农林科学

Lab Animal Pub Date : 2025-04-01 DOI: 10.1038/s41684-025-01537-0

Jorge Ferreira

引用次数: 0

Impact of obesity on infarct size, circulating biomarkers, mitochondrial function and mortality in a Göttingen minipig myocardial infarct model

IF 5.9 3区农林科学

Lab Animal Pub Date : 2025-03-27 DOI: 10.1038/s41684-025-01533-4

Mette Flethøj, Karina Poulsdóttir Debes, Cecilie Larsen, Caroline de Blanck, Trine Pagh Ludvigsen, Jeppe Kirchhoff, Jacob Eifer Møller, Steen Larsen, Jens P. Gøtze, Thomas Jespersen, Lisbeth Høier Olsen

{"title":"Impact of obesity on infarct size, circulating biomarkers, mitochondrial function and mortality in a Göttingen minipig myocardial infarct model","authors":"Mette Flethøj, Karina Poulsdóttir Debes, Cecilie Larsen, Caroline de Blanck, Trine Pagh Ludvigsen, Jeppe Kirchhoff, Jacob Eifer Møller, Steen Larsen, Jens P. Gøtze, Thomas Jespersen, Lisbeth Høier Olsen","doi":"10.1038/s41684-025-01533-4","DOIUrl":"10.1038/s41684-025-01533-4","url":null,"abstract":"Obesity is a risk factor for the development of coronary artery disease and myocardial infarction (MI). However, most large animal studies of MI are performed in lean animals. Here we assessed the impact of obesity on echocardiographic findings, infarct size, circulating biomarkers, mitochondrial respiratory capacity and mortality in a closed-chest minipig model of MI. The initial study population consisted of 20 obese (median 60.0 kg [interquartile range 55.9–64.6 kg]) and 18 lean (25.0 kg [23.4–36.5 kg]) female Göttingen minipigs. The duration of obesity induction using a western-style diet was up to approximately 6 months (156 days [24–162 days]) before the induction of MI. The induction of MI by 120-min balloon occlusion of the left anterior descending coronary artery was feasible in 17 lean and 17 obese animals. Mortality was higher in obese compared with lean animals (53% versus 12%), driven primarily by refractory ventricular fibrillation during occlusion. Electrocardiographic findings showed longer QRS and QT intervals and more extensive ST-segment elevation in obese animals compared with lean animals during occlusion. Plasma concentrations of pro-atrial natriuretic peptide, pro-C-type natriuretic peptide and cardiac troponin T were significantly lower in obese compared with lean animals. Infarct size estimated 8 weeks after MI was significantly smaller in obese (10% [9–11%]) compared with lean animals (13% [13–15%]). Finally, mitochondrial-complex-I-linked respiratory capacity was overall significantly higher in obese animals; however, no group difference was found in intrinsic mitochondrial respiratory capacity. The study shows that, compared with lean animals, obesity significantly impacts echocardiographic findings, infarct size, circulating biomarkers, mitochondrial respiratory capacity and mortality in a minipig model of myocardial infarction.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 4","pages":"103-111"},"PeriodicalIF":5.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-025-01533-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential effects of anesthetics and sex on supraventricular electrophysiology and atrial fibrillation substrate in rats

IF 5.9 3区农林科学

Lab Animal Pub Date : 2025-03-26 DOI: 10.1038/s41684-025-01532-5

Michael Murninkas, Or Levi, Sigal Elyagon, Aviv Komissar, Neta Marom, Alon Naumchik, Noam Dalal, Gideon Gradwohl, Yoram Etzion

{"title":"Differential effects of anesthetics and sex on supraventricular electrophysiology and atrial fibrillation substrate in rats","authors":"Michael Murninkas, Or Levi, Sigal Elyagon, Aviv Komissar, Neta Marom, Alon Naumchik, Noam Dalal, Gideon Gradwohl, Yoram Etzion","doi":"10.1038/s41684-025-01532-5","DOIUrl":"10.1038/s41684-025-01532-5","url":null,"abstract":"Rodents are increasingly used in atrial electrophysiology research, yet such studies are often performed under anesthesia owing to technical challenges. Here we developed an implantable device for comprehensive atrial studies in ambulatory rats and investigated the effects of commonly used anesthetics on supraventricular electrophysiology and arrhythmic substrate, comparing them with the unanesthetized state (UAS). Adult rats were evaluated 4 weeks after implantation. Studies were conducted in the UAS under 2% isoflurane (ISO) and under 40 mg/kg pentobarbital (PEN). Pacing protocols determined various parameters, including sinoatrial node recovery time, atrioventricular node effective refractory period and atrial effective refractory period. Arrhythmic substrate was assessed after 20 triggering bursts per condition, and arrhythmic tendency was analyzed manually and through the complexity ratio, an unbiased measure recently developed by our group. PEN mildly increased heart rate in both sexes, while ISO did not affect heart rate but prolonged the corrected sinus node recovery time in males. PEN increased atrioventricular node effective refractory period in both sexes, while ISO affected males only. Both ISO and PEN prolonged atrial effective refractory period compared with UAS in both sexes. Arrhythmic measures were higher in males and were attenuated by ISO and, to a lesser extent, by PEN in males only. The dominant frequency of arrhythmic events was reduced by both anesthetics in both sexes. These findings demonstrate a significant impact of commonly used anesthetics on rat supraventricular electrophysiology, with sex-based differences, highlighting the importance of methodologies that enable cardiac electrophysiology studies in unanesthetized rodents. The authors used an implantable electrophysiological device to assess the effects of two commonly used anesthetics (isoflurane and pentobarbital) on supraventricular electrophysiology and arrhythmic substrate in male and female rats.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 4","pages":"80-91"},"PeriodicalIF":5.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-025-01532-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of dietary protein are sex-dependent

IF 5.9 3区农林科学

Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01525-4

Jorge Ferreira

引用次数: 0

A new mouse model with human-like telomeres

IF 5.9 3区农林科学

Lab Animal Pub Date : 2025-03-04 DOI: 10.1038/s41684-025-01530-7

Alexandra Le Bras

引用次数: 0

NMDA receptors are involved in social facilitation of feeding behavior