Lab Animal最新文献_第2页

Dogs and humans share obesity genes 狗和人类有相同的肥胖基因

IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-05-02 DOI: 10.1038/s41684-025-01550-3

Alexandra Le Bras

{"title":"Dogs and humans share obesity genes","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01550-3","DOIUrl":"https://doi.org/10.1038/s41684-025-01550-3","url":null,"abstract":"Obesity is a global health problem mainly attributed to environmental and lifestyle factors such as diet, low physical activity or socioeconomic factors. However, genetic factors also considerably contribute to weight-gain susceptibility. Although large-scale population genomic studies in humans have identified >1,000 loci associated with body mass index, effectively narrowing down candidate genes for further mechanistic studies has been challenging. Pet dogs represent a valuable model for studying the genetic basis of obesity because, like humans, they are experiencing an obesity pandemic (40-60% of pet dogs are classified as overweight or obese), and because modern breeds are genetically homogenous, making trait mapping highly tractable. To identify obesity genes, Wallis and colleagues performed a canine genome-wide association (GWAS) study for body condition score—a measure of obesity—in 241 Labrador retrievers, a dog breed prone to obesity. From the GWAS, they identified the gene DENN domain containing 1B (DENND1B) as an obesity gene in dogs and confirmed its relevance to human obesity using data from human studies. These findings demonstrate the benefits of studying complex diseases, including obesity, in nontraditional animal models, such as the dog.Original reference: Wallis, N.J. et al. Science 387, eads2145 (2025)","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"40 1","pages":"113-113"},"PeriodicalIF":6.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AAV toolkit for axolotl nervous system 蝾螈神经系统AAV工具包

IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-05-02 DOI: 10.1038/s41684-025-01551-2

Alexandra Le Bras

{"title":"AAV toolkit for axolotl nervous system","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01551-2","DOIUrl":"https://doi.org/10.1038/s41684-025-01551-2","url":null,"abstract":"The axolotl, which can regenerate injuries to the central and peripheral nervous system, is an emerging model system to study neural circuit regeneration. Most findings in this model come from using classical tracers and imaging techniques, which present important limitations. Therefore, there is a need for new tools to efficiently label and/or manipulate cells in the axolotl nervous system and capture neural circuit regeneration. Studies in rodents and other mammals have frequently used recombinant adeno-associated viruses (AAVs) for targeted gene delivery in the nervous system, facilitating both circuit identification and functional circuit analysis. However, there are still no reports of AAV-mediated gene delivery to axolotl nervous system tissue. A new study in PNAS presents a set of AAV serotypes for efficient gene delivery in the axolotl nervous system, which could facilitate the study of nervous system regeneration. The findings notably show that while AAV8, AAV9, AAVRG and AAVPHP.eB are most suited to label neurons of the axolotl brain, AAV8, AAV9, and AAVPHP.eB are also suited to label cells of the spinal cord, with AAV9 being also the most suitable to label retinal cells.Original reference: Lust, K. & Tanaka, E.M. Proc. Natl. Acad. Sci. USA 122, e2421373122 (2025)","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"224 1","pages":"113-113"},"PeriodicalIF":6.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of intermittent fasting on male sexual behavior. 间歇性禁食对男性性行为的影响。

IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-05-01 DOI: 10.1038/s41684-025-01557-w

Alexandra Le Bras

引用次数: 0

Mice also rely on visual cues for social behavior. 老鼠的社会行为也依赖于视觉线索。

IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-05-01 DOI: 10.1038/s41684-025-01558-9

Jorge Ferreira

引用次数: 0

Ghrelin's role in mouse anxiety. 胃饥饿素在小鼠焦虑中的作用。

IF 5.9 3区农林科学

Lab Animal Pub Date : 2025-05-01 DOI: 10.1038/s41684-025-01559-8

Jorge Ferreira

引用次数: 0

Intranasal delivery of drugs to the central nervous system of adult zebrafish 成年斑马鱼中枢神经系统的鼻内药物输送

IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-04-30 DOI: 10.1038/s41684-025-01545-0

David S. Galstyan, Tatyana O. Kolesnikova, Konstantin A. Demin, Yaroslav A. Dubrovskii, Ekaterina Murashko, Elizaveta Kessenikh, Nikita P. Ilyin, Aleksey N. Ikrin, Anastasia M. Moskalenko, Murilo S. de Abreu, Longen Yang, Allan V. Kalueff

{"title":"Intranasal delivery of drugs to the central nervous system of adult zebrafish","authors":"David S. Galstyan, Tatyana O. Kolesnikova, Konstantin A. Demin, Yaroslav A. Dubrovskii, Ekaterina Murashko, Elizaveta Kessenikh, Nikita P. Ilyin, Aleksey N. Ikrin, Anastasia M. Moskalenko, Murilo S. de Abreu, Longen Yang, Allan V. Kalueff","doi":"10.1038/s41684-025-01545-0","DOIUrl":"https://doi.org/10.1038/s41684-025-01545-0","url":null,"abstract":"The small teleost zebrafish (Danio rerio) has become a critically important laboratory animal in biomedicine. One of their key practical advantages, the convenient method of small-molecule administration via water immersion, has certain problems with dosing precision and drug delivery. Here, we present a simple protocol for the intranasal delivery of neuroactive drugs in adult zebrafish using arecoline and nicotine, two well-studied reference neuroactive drugs chosen for the proof of concept. Adult fish received 1 μL water solution of arecoline (1 and 10 mg/mL) or nicotine tartrate (5 and 10 mg/mL) or the same volume of drug-free water (control) into both nostrils, followed by behavioral testing in the novel tank test 5 min later. Mass spectrometry analyses confirmed that both drugs rapidly reached the zebrafish brain following intranasal administration. Intranasally administered arecoline (10 mg/mL) and nicotine (5 and 10 mg/mL) demonstrated overt behavioral profiles, evoking characteristic anxiolytic-like effects in zebrafish similar to those observed here for a standard 20-min water immersion method (10 mg/L arecoline or 30 mg/L nicotine). Overall, we showed that neuroactive drugs can be delivered to adult zebrafish intranasally to exert central effects, which may (at least for some drugs) occur faster and can need smaller drug quantities than for the water immersion delivery.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"71 1","pages":""},"PeriodicalIF":6.9,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143889894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parent-of-origin of alleles: an essential variable in in vivo experiments 等位基因的亲本来源：体内实验中的一个重要变量

IF 6.9 3区农林科学

Lab Animal Pub Date : 2025-04-02 DOI: 10.1038/s41684-025-01546-z

Lydia Teboul, Michelle E. Stewart

引用次数: 0

Role for neutrophils in endometriosis 中性粒细胞在子宫内膜异位症中的作用

IF 5.9 3区农林科学

Lab Animal Pub Date : 2025-04-01 DOI: 10.1038/s41684-025-01538-z

Alexandra Le Bras

引用次数: 0

Fibrosis considerations for liver gene therapy 肝基因治疗对纤维化的考虑