Journal of Veterinary Research最新文献

{"title":"Prevalence, molecular identification and genotyping of the crayfish plague pathogen, <i>Aphanomyces astaci</i> in major narrow-clawed crayfish (<i>Pontastacus leptodactylus</i> Eschscholtz, 1823) populations from Türkiye.","authors":"Süleyman Akhan, İfakat Tülay Çağatay, Selçuk Berber, Büşra Taştan, Yiğit Taştan, Tuba Dalar","doi":"10.2478/jvetres-2024-0061","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0061","url":null,"abstract":"<p><strong>Introduction: </strong>Crayfish plague is considered the most important crayfish disease globally. It is caused by the fungus-like agent, <i>Aphanomyces astaci</i>. This study aimed to identify and determine the prevalence of <i>A. astaci</i> using PCR in narrow-clawed crayfish (<i>Pontastacus leptodactylus</i>) populations from across Türkiye.</p><p><strong>Material and methods: </strong>A PCR was carried out with primers specific to the internal transcribed spacer region of the <i>A. astaci</i> pathogen on both telson and abdominal cuticle tissues from crayfish individuals from 41 different locations.</p><p><strong>Results: </strong><i>Aphanomyces astaci</i> was detected in the crayfish from 34 of the locations. Molecular diagnosis showed the prevalence rates of <i>A. astaci</i> to be between 0% and 68.2%. For 7 of the 34 locations, the strain of <i>A. astaci</i> was determined. Microsatellite analysis of tissue from individuals with positive PCR results revealed the <i>A. astaci</i> genotypes in seven populations. Genotype B was found to be the predominant genotype responsible for crayfish plague in Turkish crayfish populations. The Psl genotype (genotype B) was determined in six of the populations, and the As genotype (genotype A) was detected in only one.</p><p><strong>Conclusion: </strong>Crayfish plague poses a significant threat to crayfish populations, necessitating the development of rapid, highly sensitive diagnostic methods. An understanding of the sensitivity of the PCR detection method and of the prevalence and genotyping of <i>A. astaci</i> in Turkish crayfish populations has been gained from this study.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"515-524"},"PeriodicalIF":1.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The grey wolf <i>(Canis lupus)</i> as a host of <i>Echinococcus multilocularis, E. granulosus s.l</i>. and other helminths - a new zoonotic threat in Poland.","authors":"Jacek Karamon, Małgorzata Samorek-Pieróg, Ewa Bilska-Zając, Weronika Korpysa-Dzirba, Jacek Sroka, Jolanta Zdybel, Tomasz Cencek","doi":"10.2478/jvetres-2024-0060","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0060","url":null,"abstract":"<p><strong>Introduction: </strong>The aim of this study was to estimate the occurrence of <i>Echinococcus</i> spp. and other helminth infections in grey wolves in south-eastern Poland.</p><p><strong>Material and methods: </strong>Overall, 74 samples of wolf faeces were examined with a multiplex PCR and a system of real-time quantitative PCR methods to detect and identify <i>Echinococcus</i> spp. The faeces were additionally examined microscopically. Also, 20 samples of wolf intestines were examined with a sedimentation and counting technique (SCT).</p><p><strong>Results: </strong><i>Echinococcus multilocularis</i> DNA was detected in 6.8% and <i>E. granulosus s.l</i>. (identified as <i>E. ortleppi</i>) in 4.1% of faeces samples. <i>Taenia</i> spp. DNA was found in 43.2% and <i>Mesocestoides</i> in 4.1%. Examination of the intestines by SCT showed <i>E. multilocularis</i> worms in 10%, <i>E. granulosus s.l</i>. (<i>E. ortleppi</i>) in10%, <i>Taenia</i> spp. in 100%, hookworms in 30%, <i>Alaria alata</i> in 20%, <i>Mesocestoides</i> sp. in 10%, <i>Trichuris vulpis</i> in 15%, <i>Molineus</i> sp. in 5% and <i>Euryhelmis</i> sp. in 5%. By coproscopy, Capillariidae eggs were found in 59% of faeces samples. Genetic analysis of <i>E. multilocularis</i> worms showed the presence of two European haplotypes previously described in Poland in red foxes and pigs. Sequences of <i>nad1</i> obtained from <i>E. ortleppi</i> worms shared full identity with a sequence from a human case in Poland.</p><p><strong>Conclusion: </strong>The study showed the presence of <i>E. multilocularis</i> in wolves for the first time in Poland and confirmed our earlier observations on <i>E. ortleppi</i>. This double threat from <i>Echinococcus</i> in this wolf population should be taken into account when assessing the epidemiological risk. The study enriched the knowledge of other helminths found in wolves, also those (<i>Euryhelmis</i>) that were recorded for the first time in this species.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"539-549"},"PeriodicalIF":1.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of probiotic administration on selected leukocyte subpopulations and the serum amyloid A concentration in the peripheral blood of dairy cows during different lactation periods.","authors":"Piotr Brodzki, Jan Marczuk, Hubert Gorzkoś, Urszula Lisiecka, Marek Szczubiał, Adam Brodzki, Roman Dąbrowski, Leszek Krakowski, Katarzyna Głodkowska, Nikodem Brodzki","doi":"10.2478/jvetres-2024-0057","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0057","url":null,"abstract":"<p><strong>Introduction: </strong>The aim of the study was to compare selected leukocyte subpopulations and the serum amyloid A (SAA) concentration in the peripheral blood of cows at different stages of lactation. The blood of cows receiving a probiotic as a dietary supplement was compared with the blood of cows not receiving it.</p><p><strong>Material and methods: </strong>The research was conducted on 20 pregnant dairy cows randomly divided into two groups of 10 cows each. The experimental group consisted of cows given the probiotic as a feed supplement. The control group consisted of cows that were fed without supplementation. Blood was drawn six times for testing: 7 days before drying; 14 days before parturition; and 7, 21, 60 and 90 days postpartum. Leukocyte immunophenotyping was performed by flow cytometry.</p><p><strong>Results: </strong>The blood of cows administered the probiotic revealed an increased percentage of forkhead box protein 3 (Foxp3)⁺, T CD4⁺ and B CD25⁺ lymphocytes and β2 CD18⁺ and αM CD11b⁺ integrins, and persistently low SAA levels at all time points.</p><p><strong>Conclusion: </strong>The activity of the immune system in cows receiving the probiotic was higher than in control cows. However, the stabilisation of the immune system of the supplemented cows may be indicated by the persistence of a low level of SAA throughout the experiment. Therefore, it can be assumed that the immune system of cows treated with the probiotic more easily adapts to changes in conditions in particular lactation periods and that these cows become more resistant to infectious diseases.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"589-597"},"PeriodicalIF":1.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of prenatal fumonisin B exposure on bone innervation in newborn Wistar rats.","authors":"Ewa Tomaszewska, Piotr Dobrowolski, Aleksandra Dajnowska, Liwia Arbatowska, Iwona Puzio, Halyna Rudyk, Oksana Brezvyn, Ihor Kotsyumbas, Janine Donaldson, Jadwiga Śliwa, Marcin B Arciszewski, Siemowit Muszyński","doi":"10.2478/jvetres-2024-0056","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0056","url":null,"abstract":"<p><strong>Introduction: </strong>This study explored the effects of prenatal exposure to fumonisins B (FB) on bone innervation in newborn Wistar rats.</p><p><strong>Material and methods: </strong>Pregnant dams (n = 6 per group) were assigned to either the control or one of two FB-exposed groups (60 mg or 90 mg/kg body weight) from the 7^th day of gestation until parturition. On the day of parturition, one male pup from each litter (n = 6 per group) was randomly selected and euthanised, and their femurs were dissected for analysis. Bone innervation was quantified by examining the morphology patterns of sympathetic, parasympathetic, sensory and cocaine- and amphetamine-regulated transcript (CART)-positive fibres. Prepared bone sections were analysed using immunohistochemistry staining for protein gene product 9.5, tyrosine hydroxylase, choline acetyltransferase, vasoactive intestinal peptide (VIP), substance P and CART-positive neurons.</p><p><strong>Results: </strong>The group that received a higher dose of FB demonstrated an increase in both the size and complexity of the complete bone neuronal network together with heightened sympathetic and sensory innervation, and displayed a decrease in neuron density and sympathetic innervation. Fumonisin B exposure led to a decrease in galanin-positive and VIP-positive bone neuronal networks in both groups exposed to FB, while in the lower-dose group, there was also a decrease in CART-positive innervation.</p><p><strong>Conclusion: </strong>Prenatal FB exposure significantly influences the neuronal bone network of rats, which is essential for maintaining bone homeostasis. These findings emphasise the necessity for further research to understand the lasting effects and underlying mechanisms of alterations induced by FB.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"633-642"},"PeriodicalIF":1.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of consumption of cranberry <i>(Vaccinium macrocarpon)</i> on <i>Escherichia coli</i> adherence to feline uroepithelial cells in a blind randomised cross-over trial in cats.","authors":"Amaranta Carvajal-Campos, Lucile Trebossen, Isabelle Jeusette, Gilles Mayot, Celina Torre, Victor Fragua, Anna Fernandez, Patrick Di Martino","doi":"10.2478/jvetres-2024-0055","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0055","url":null,"abstract":"<p><strong>Introduction: </strong><i>Escherichia coli</i> is the most common uropathogen in humans, dogs and cats. Dietary consumption of cranberry (<i>Vaccinium macrocarpon</i>) is known to be associated with a reduction in uropathogenic <i>E. coli</i> (UPEC) adhesion to human and canine urinary epithelial cell lines, but this has not been shown in cats.</p><p><strong>Material and methods: </strong>Six neutered domestic cats, one male and five females, were randomly fed three diets successively, one containing 0.1% cranberry powder, one containing 0.3% cranberry powder, and one being the control without cranberry. Naturally emitted urine was collected on the last two days of each period of two weeks and used for bacterial growth. Adherence to Crandell-Rees feline kidney (CRFK) uroepithelial cells of the feline UPEC C571 strain (positive for the <i>pap</i>C gene marker for P-fimbriae and the <i>fim</i>A marker for type 1 pili and negative for the gene of the alpha haemolysin cytotoxin <i>hly</i>A, and additionally non-haemolytic <i>in vitro</i> on blood agar) was quantified after growth in urine samples.</p><p><strong>Results: </strong>Significant reductions in bacterial adherence to CRFK cells were observed for 60% of cats receiving 0.1% cranberry powder supplementation and for all cats receiving 0.3% cranberry powder supplementation, compared to the same animals consuming the control diet.</p><p><strong>Conclusion: </strong>Dietary supplementation with cranberry may provide some degree of protection to cats against adhesion of UPEC to feline uroepithelial cells.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"583-587"},"PeriodicalIF":1.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular analysis of <i>Anaplasma ovis, Theileria ovis</i> and <i>Brucella abortus</i> in adult <i>Ornithodoros lahorensis</i> soft ticks (Acari: Ixodida: Argasidae) isolated from the Xinjiang Uygur Autonomous Region, China.","authors":"Dandan Liu, Jinming Wang, Yutong Liu, Shuiyi Wang, Huiru Zhu, Bingbing Jiang, Yongchang Li, Yang Zhang, Bayin Chahan, Wei Zhang","doi":"10.2478/jvetres-2024-0049","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0049","url":null,"abstract":"<p><strong>Introduction: </strong>Ticks are obligate blood-feeding arthropods that cause significant economic losses in domestic animal husbandry and threaten public health. However, information about soft ticks (Acari: Argasidae) and tick-borne pathogens in the Xinjiang Uygur Autonomous Region (XUAR) of China is scarce.</p><p><strong>Material and methods: </strong>In this study, PCR assays and gene sequencing were used to detect and analyse the epidemiological features of <i>Anaplasma ovis</i>, <i>Theileria ovis</i> and <i>Brucella abortus</i> parasitic infections in 366 <i>Ornithodoros lahorensis</i> soft ticks collected from five sampling sites in the XUAR from October 2019 to March 2022. The ticks were identified by morphological and molecular methods as <i>O. lahorensis</i>. The PCR was conducted using primers complementary to the major surface protein 4 (<i>Msp4</i>) gene of <i>A. ovis</i>, the 18S ribosomal RNA (18S rRNA) of <i>T. ovis</i> and the outer membrane protein 22 (<i>Omp22</i>) gene of <i>B. abortus</i>.</p><p><strong>Results: </strong>The overall infection rate was 91/366 (24.9%) for <i>A. ovis</i>, 127/366 (34.7%) for <i>T. ovis</i> and 94/366 (25.6%) for <i>B. abortus</i>. Sequencing analysis indicated that <i>A. ovis Msp4</i>, <i>T. ovis</i> 18S rRNA and <i>B. abortus Omp22</i> genes from XUAR isolates showed 99.58-100% identity with documented isolates from other countries.</p><p><strong>Conclusion: </strong>This study provides fundamental evidence for the occurrence of <i>A. ovis</i>, <i>T. ovis</i> and <i>B. abortus</i> in <i>O. lahorensis</i>. Therefore, the potential threat of soft ticks to livestock and humans should not be ignored. This study expands the understanding of the existence of tick-borne pathogens in <i>O. lahorensis</i> and is expected to improve the strategies for prevention and control of ticks and tick-borne diseases in China.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 3","pages":"355-361"},"PeriodicalIF":1.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the addition of different forms of rosemary (<i>Rosmarinus officinalis</i> L.) on the quality of vacuum-packed minced pork.","authors":"Katarzyna Śmiecińska, Tomasz Daszkiewicz, Agnieszka Krajewska, Dorota Kubiak","doi":"10.2478/jvetres-2024-0047","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0047","url":null,"abstract":"<p><strong>Introduction: </strong>Apart from their antioxidant activity, plant-derived bioactive compounds can also positively affect the quality of meat and meat products by improving their sensory and microbiological properties and preventing discolouration. The aim of this study was to determine how the addition of different forms of rosemary improved the quality of pork.</p><p><strong>Material and methods: </strong>Minced pork samples were divided into a control sample without additives (C) and three experimental samples with certified additives (15 mg/kg of meat each), <i>i.e</i>. rosemary oleoresin (ROL), extract (REX) and essential oil (REO). Each was further divided into three subsamples; the first was evaluated before storage, and the second and third were evaluated after respective 7- and 14-day vacuum-packed storage at 2°C. The TBARS value was expressed as mg of malondialdehyde (MDA) per kg of meat. Colour was determined based on the values of the standard colour space values of L* (lightness), a* (redness) and b* (yellowness) as well as C* (chroma) and h° (hue angle). Sensory attributes of the samples were evaluated on a nine-point scale. The pour-plating procedure was used for the enumeration of <i>Pseudomonas</i>, mesophilic lactic acid bacteria, psychrotrophic bacteria and rods of the <i>Enterobacteriaceae</i> family. Within each bacterial group, the most common colonies were identified by matrix-assisted laser desorption and ionization (MALDI).</p><p><strong>Results: </strong>Lipid oxidation was most effectively inhibited by REO. The addition of ROL and REO to pork lightened its colour. Meat with REO had stronger redness, whereas meat with ROL had stronger yellowness. The addition of REX affected the sensory properties of pork most beneficially. Neither <i>Enterobacteriaceae</i> nor <i>Pseudomonas</i> spp. were detected in REO pork, which also contained lower counts of lactic acid bacteria than group C pork.</p><p><strong>Conclusion: </strong>The results of this study indicate that rosemary has antioxidant and antimicrobial properties, and may improve the colour and sensory attributes of pork. The effect exerted by rosemary on meat quality may vary depending on the physical form of the additive.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 3","pages":"419-426"},"PeriodicalIF":1.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of chokeberry pomace as a feed additive for high-producing dairy goats on oxidative stress parameters and quality of milk.","authors":"Monika Szymańska-Czerwińska, Maima Matin, Krzysztof Niemczuk, Nina Strzałkowska, Zbigniew Osiński, Karina Horbańczuk, Agnieszka Wierzbicka, Jarosław Olav Horbańczuk, Atanas Georgiev Atanasov, Artur Jóźwik","doi":"10.2478/jvetres-2024-0052","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0052","url":null,"abstract":"<p><strong>Introduction: </strong>Chokeberry pomace, rich in polyphenolic compounds, holds potential to be a valuable feed additive for enhancing the antioxidative capacity and overall quality of milk. This study explores the impact of dietary inclusion of chokeberry pomace on oxidative stress parameters and other milk quality parameters in high-producing dairy goats.</p><p><strong>Material and methods: </strong>Twenty-seven goats were allocated into three groups: a control group provided standard feed and two experimental groups provided feed supplemented with 15 g or 30 g of chokeberry pomace per kilogram. Milk samples were analysed for physicochemical traits, a range of enzyme activities and antioxidant properties.</p><p><strong>Results: </strong>Supplementation with chokeberry pomace significantly reduced milk fat content, enhanced antioxidative properties and increased most of the quantified enzyme activities. Total polyphenol content and reduced glutathione levels were significantly higher in the supplemented groups, correlating with improved antioxidative potential of the milk.</p><p><strong>Conclusion: </strong>Chokeberry pomace in goat diets enhances milk's antioxidative properties and upregulates its enzymatic activity profile, suggesting a potential strategy to improve the nutritional quality and health benefits of goat milk. The study underscores the utility of chokeberry pomace as a feed additive that might not only benefit animal health but also contribute to enhanced milk quality.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 3","pages":"409-417"},"PeriodicalIF":1.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prevalence of <i>Mycoplasma canis</i> in the vaginas of breeding bitches.","authors":"Dariusz Jagódka, Edyta Kaczorek-Łukowska, Piotr Andrzej Socha","doi":"10.2478/jvetres-2024-0054","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0054","url":null,"abstract":"<p><strong>Introduction: </strong>How bacterial infections of the reproductive tract cause infertility and the correlation between the health status of female dogs and the presence of <i>Mycoplasma canis</i> (<i>M. canis</i>) in the vagina are still unclear. The aim of this study was to determine the <i>M. canis</i> population in the vagina of breeding bitches and to correlate this microbial population with some fertility outcomes.</p><p><strong>Material and methods: </strong>A total of 275 breeding bitches were included in the study. Vaginal samples were collected for microbiological and PCR testing.</p><p><strong>Results: </strong><i>Mycoplasma canis</i> was identified in 34.91% of the samples. One-third of bitches from the problem-free group and 41.18% from the group with problems were positive. In general, there were no significant differences in the prevalence of <i>M. canis</i> between the groups (P-value > 0.05). <i>Mycoplasma canis</i> occurs in both mated and unmated bitches and was found in a large number of kennels (67%). There was a correlation between <i>M. canis</i> in the kennel and the incidence of single puppy deaths and low litter sizes. There was also some correlation between the presence of <i>M. canis</i> in the vagina with at least two other bacterial strains and reproductive disorders.</p><p><strong>Conclusion: </strong>Our results indicate that <i>M. canis</i> is part of the normal vaginal flora of breeding bitches, although a role for this bacterium in causing some reproductive disorders remains to be disproved.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 3","pages":"347-353"},"PeriodicalIF":1.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the role of <i>Dirofilaria repens</i> macrophage migration inhibitory factors in host-parasite interactions.","authors":"Justyna Karabowicz, Ewa Długosz, Piotr Bąska, Mateusz Pękacz, Magdalena Elżbieta Wysmołek, Maciej Klockiewicz, Marcin Wiśniewski","doi":"10.2478/jvetres-2024-0038","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0038","url":null,"abstract":"<p><strong>Introduction: </strong><i>Dirofilaria repens</i> is a zoonotic parasitic filarial nematode that infects carnivores and occasionally humans. Knowledge of the host-parasite molecular interactions enabling the parasite's avoidance of the host immune response in subcutaneous dirofilariasis remains limited. Parasitic orthologues of host macrophage migration inhibitory factor (MIF) are molecules potentially involved in this process.</p><p><strong>Material and methods: </strong>Complementary DNA encoding two <i>D. repens</i> MIF orthologues (r<i>Dre</i>-MIF-1 and r<i>Dre</i>-MIF-2) was cloned into a pET-28a expression vector. The recombinant proteins were produced in <i>Escherichia coli</i> and purified using affinity nickel chromatography. The reactivity of both recombinant proteins was analysed with infected dog and immunised mouse sera.</p><p><strong>Results: </strong>Stronger antibody production was induced by r<i>Dre-</i>MIF-1 in mice, as evidenced by significantly higher levels of anti-r<i>Dre</i>-MIF-1 total IgG, IgG2 and IgE antibodies than of anti-r<i>Dre</i>-MIF-2 immunoglobulins. Additionally, a significantly different level of antibodies specific to both proteins was noted between the sera of infected dogs and those of uninfected dogs.</p><p><strong>Conclusion: </strong>This study is the first attempt to characterise MIF orthologues from the filarial parasite <i>D. repens</i>, which may affect the immune response during infection.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 3","pages":"381-388"},"PeriodicalIF":1.3,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}