Journal of Veterinary Research最新文献

{"title":"Is <i>Pseudomonas aeruginosa</i> a possible aetiological agent of periodontitis in dogs?","authors":"Małgorzata Targońska-Karasek, Izabela Polkowska, Henryk Krukowski","doi":"10.2478/jvetres-2025-0006","DOIUrl":"10.2478/jvetres-2025-0006","url":null,"abstract":"<p><strong>Introduction: </strong>Periodontal diseases are the most frequently diagnosed problem in small animal veterinary medicine. Although their exact cause is not fully understood, bacteria play an important role in their development. <i>Pseudomonas aeruginosa</i> is a Gram-negative, rod-shaped, non-spore-forming bacterium. The living environment of this bacterium may be soil and water; however, it can also be found in humans and animals. Antibiotic treatment of periodontitis may be complicated by the carbapenem resistance of some <i>P. aeruginosa</i> strains, if these bacteria are found to be an aetiological agent. The aim of the study was to identify all bacterial strains isolated from dog with periodontitis.</p><p><strong>Material and methods: </strong>After a clinical examination of a Schnauzer dog in the Department and Clinic of Animal Surgery in the University of Life Sciences in Lublin Faculty of Veterinary Medicine, periodontitis was diagnosed. A swab was taken from the diseased tissue and submitted for microbiological tests. Microorganisms were initially identified by colony morphology, haemolytic pattern and Gram staining, and subsequently by sensitivity tests, VITEK 2 and matrix-assisted laser desorption/ionisation-time-of-flight.</p><p><strong>Results: </strong><i>Pseudomonas aeruginosa</i> was isolated and identified as a probable aetiological factor of periodontitis in dogs.</p><p><strong>Conclusion: </strong>In our opinion, attention should be paid to <i>Pseudomonas aeruginosa</i> as a possible aetiological factor of periodontal diseases in dogs.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"35-40"},"PeriodicalIF":1.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of body condition score loss during the transition period on metabolism, milk yield and health in Holstein cows.","authors":"Rui Sun, Xuejie Jiang, Yu Hao, Ying Li, Yunlong Bai, Cheng Xia, Yuxi Song","doi":"10.2478/jvetres-2025-0004","DOIUrl":"10.2478/jvetres-2025-0004","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to investigate the impact of perinatal body condition score (BCS) and its subsequent loss on postpartum performance and health outcomes in dairy cattle.</p><p><strong>Material and methods: </strong>A total of 156 cows were randomly selected, and blood samples were collected at -21, 0, 7, 14, 21, 28 and 50 days relative to calving. Milk yield and disease incidence in dairy cows were recorded after calving. These cows were subsequently categorised into three groups based on BCS loss during the transition period: a no-BCS-loss (maintained BCS) group (M, 0 < BCS loss ≤ 0.25), low-BCS-loss group (L, 0.25 < BCS loss ≤ 0.5), and high-BCS-loss group (H, BCS loss > 0.5).</p><p><strong>Results: </strong>All groups experienced a decline in BCS from 21 days prepartum through 50 days postpartum (P-value < 0.01). Cows in the H group had the highest levels of non-esterified fatty acids, beta-hydroxybutyrate, total cholesterol, aspartate aminotransferase, albumin, malondialdehyde and leptin (P-value < 0.05). Concomitantly, total antioxidant capacity, as well as the levels of insulin and glucose, were the lowest in group H (P-value < 0.05). Plasma concentrations of Ca, P, Mg and K, urea nitrogen and total bilirubin were not significantly influenced by BCS loss (P-value > 0.05). Cows in the M group were less likely to develop ketosis, mastitis, retained placenta, displaced abomasum and metritis than those in the H group, and cows in the H group produced the lowest milk yields (P-value < 0.05).</p><p><strong>Conclusion: </strong>These observations collectively indicate that BCS loss is associated with measurable changes in energy balance, liver function, oxidative stress, daily milk production and disease incidence during the transition period.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"91-99"},"PeriodicalIF":1.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remodelling of the healthy foal's conjunctival microbiome in the first two months of life.","authors":"Katarzyna Płoneczka-Janeczko, Eve Armstrong, Marta Siemieniuch-Tartanus, Marcin Magdziarz","doi":"10.2478/jvetres-2025-0001","DOIUrl":"10.2478/jvetres-2025-0001","url":null,"abstract":"<p><strong>Introduction: </strong>The aim of the study was to explore and characterise healthy foals' eye microbiomes in the first two months of life.</p><p><strong>Material and methods: </strong>Conjunctival swabs were collected three times, not later than 12 h after delivery and again at the end of the first and the second months of life from six clinically healthy foals of the Polish Konik breed. The average interval between the first and second samplings was 33.3 days and between the second and third was 35.6 days. Next-generation sequencing performed on a MiSeq sequencer in paired-end technology was used to analyse the composition of the conjunctival microbiota.</p><p><strong>Results: </strong>Paired one-sided <i>t</i>-tests revealed that conjunctival microbiota diversity was the lowest in the first 24 h of life and significantly increased between birth and the first month. The most prevalent family throughout the study was <i>Micrococcaceae</i> and the most prevalent genus was <i>Corynebacterium</i>. Sequences of potentially pathogenic bacteria such as <i>Pseudomonas, Acinetobacter</i> and <i>Streptococcus</i> spp. that may be involved in inflammatory processes were identified. Ocular commensals such as <i>Corynebacterium</i> and <i>Lactobacillaceae</i> that were found in the ocular surface microbiome of the foals are believed to be capable of restoring the ocular microbiome and maintaining balance.</p><p><strong>Conclusion: </strong>A healthy ocular surface microbiota in the early period of a foal's life develops dynamically and changes its composition.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"131-140"},"PeriodicalIF":1.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of neurokinin B, dynorphin A and kisspeptin-10 on <i>in vitro</i> gonadotropin secretion by anterior pituitary cells isolated from pubescent ewes.","authors":"Natalia Szysiak, Urszula Kosior-Korzecka, Vincenzo Longo, Krzysztof Patkowski, Monika Greguła-Kania, Aneta Nowakiewicz, Mariola Bochniarz, Andrzej Junkuszew","doi":"10.2478/jvetres-2025-0003","DOIUrl":"10.2478/jvetres-2025-0003","url":null,"abstract":"<p><strong>Introduction: </strong>The hypothalamic neuropeptides kisspeptin-10 (KiSS-10), neurokinin B (NKB), and dynorphin A (Dyn A) play roles in the endocrine regulation of the hypothalamic-pituitary-ovarian (HPO) axis in puberty. Livestock's timely attainment of sexual maturity increases reproductive efficiency and raises profitability. The pituitary relationship between these neuropeptides and gonadotropins in puberty in ewes being undercharacterised. The aim of the study was to analyse their direct effect on gonadotropin secretion by pituitary cells isolated from pubescent ewes.</p><p><strong>Material and methods: </strong>Cells were incubated in McCoy's 5A medium, either without neuropeptides (as the control) or with 10^-11, 10^-10, 10^-9, 10^-8 and 10^-7 M of KiSS-10, NKB and Dyn A. After 4, 12 and 24 h, the luteinising hormone (LH) and follicle-stimulating hormone (FSH) concentrations were analysed by ELISA using species-specific antibodies.</p><p><strong>Results: </strong>Greater LH and FSH secretion was observed after the 4-24 h exposure to respective 10^-11-10^-8 M and 10^-11-10^-7 M concentrations of KiSS-10. Moreover, NKB and Dyn A applied in the concentration range elevated the secretion of both LH and FSH throughout the experiment. Dynorphin A had the most significant effect on gonadotropin secretion at all the concentrations used. In contrast, the most pronounced dose-dependent neuropeptide effect throughout the experiment on the FSH secretion was attributed to NKB.</p><p><strong>Conclusion: </strong>Kisspeptin-10, NKB and Dyn A had a direct impact on gonadotropin secretion by ovine pituitary cells. However, a detailed explanation of their role in gonadotropin secretion by the anterior pituitary gland in sheep and of their impact on the regulation of the HPO axis during sexual maturation or in the pathomechanism of delayed puberty require further studies.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"121-129"},"PeriodicalIF":1.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution, lipophilicity and tissue half-life as key factors in sulphonamide clearance from porcine tissues.","authors":"Artur Burmańczuk, Monika Osypiuk, Bożena Polska, Dominik Kunicki, Marcin Kocik, Karol Grzęda, Włodzimierz Markiewicz, Oktay Yilmaz, Tomasz Grabowski","doi":"10.2478/jvetres-2025-0002","DOIUrl":"10.2478/jvetres-2025-0002","url":null,"abstract":"<p><strong>Introduction: </strong>Sulphonamides are some of the most widely used antimicrobial drugs in the treatment of bacterial diseases in pigs. The study was conducted to compare the total exposure of tissue to a group of active substances in a single formulation and evaluate the impact of the volume of distribution, lipophilicity and tissue half-life of sulfadimethoxine, sulphathiazole, sulphamethazine and sulphacetamide in two different veterinary drug formulations - Polisulfalent, which was preparation A, and Polisulfamid, which was preparation B.</p><p><strong>Material and methods: </strong>Each tested therapeutic preparation was administered to 15 piglets of the Polish Landrace breed. To assume general exposure expressed as the sum of all observed concentrations of all active substances, semi-log regression analyses were performed for both formulations.</p><p><strong>Results: </strong>The estimated tissue half-life was 21.19 h for preparation A and 17.36 h for preparation B. The comparison of the semi-log regression parameters shows that formulation B's Y-intercept and slope values were higher than formulation A's. The upper-bound CI for the Y-intercept and slope value of formulation B was higher than those of formulation A, at 38.7 and 20.8%, respectively.The lower-bound CI for the Y-intercept and slope value of formulation B was also higher than those of formulation A, at 20.6 and 42.9%, respectively.</p><p><strong>Conclusion: </strong>Current observations lead to the conclusion that in late-stage drug elimination, it is not the blood half-life, but rather a drug's volume of distribution which is key in determining the clearance period, this volume being dependent on the physicochemical characteristics of the drug.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"69 1","pages":"101-109"},"PeriodicalIF":1.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11949273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of chlamydiae in dairy cattle herds and factors contributing to the spread of infections.","authors":"Mońika Szymańska-Czerwińska, Agnieszka Jodełko, Zbigniew Osiński, Kinga Zaręba-Marchewka, Krzysztof Niemczuk","doi":"10.2478/jvetres-2024-0071","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0071","url":null,"abstract":"<p><strong>Introduction: </strong>Different <i>Chlamydia</i> species affect cattle and contribute to economic losses. One of them, <i>C. pecorum</i>, is a globally endemic livestock pathogen. Despite its endemicity, prevalence data from Poland have so far been limited. The present study aimed to obtain insight into the chlamydiae prevalence in Polish dairy cattle.</p><p><strong>Material and methods: </strong>A screening of chlamydial seroprevalence in dairy cattle was initially performed, followed by <i>Chlamydiaceae</i>- and species-specific real-time qPCR. Vaginal swabs (n = 239) and placenta samples (n = 2) from seropositive animals in 142 herds were collected to detect shedders. The study population consisted of cows (n = 2,780) from dairy herds (n = 1,153) located in all Polish voivodeships.</p><p><strong>Results: </strong>The true animal prevalence was determined to be 33.3%, while the true herd prevalence was 42.7%. Five groups of Polish voivodeships were identified using appropriate statistical tools, highlighting differences that may arise from various factors impacting the spread of chlamydial infections. The only detected chlamydia species was <i>C. pecorum</i>, the presence of which was confirmed in two herds.</p><p><strong>Conclusion: </strong>This study revealed that chlamydial infections are commonly present in Polish dairy cattle across the country.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"531-538"},"PeriodicalIF":1.3,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of clostebol residues in the urine of slaughter animals using liquid chromatography-tandem mass spectrometry.","authors":"Iwona Matraszek-Żuchowska, Alicja Kłopot, Justyna Grzelak, Paulina Zdonek","doi":"10.2478/jvetres-2024-0070","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0070","url":null,"abstract":"<p><strong>Introduction: </strong>Synthetic anabolic hormones, which may pose a potential risk to human health, should not be used in fattening food-producing animals. Because of the hormonal effects they cause, growth-promoting compounds are banned by legislation in the EU for use in animal husbandry. Consequently, all EU member states are required to conduct monitoring tests on the content and residues of these compounds in prescribed biological matrices to ensure the safety of food consumers. The aim of this research was to develop a liquid chromatography-tandem mass spectrometry method for the detection of the residue of one such anabolic hormone, clostebol in food animal urine.</p><p><strong>Material and methods: </strong>Clostebol and its marker metabolite residues were determined by a method involving enzymatic hydrolysis, isolation of compounds from urine on a C18 solid-phase extraction (SPE) column, purification of the extract by liquid-liquid extraction using n-pentane and a NH₂ SPE column, and detection by liquid chromatography-tandem mass spectrometry.</p><p><strong>Results: </strong>No traces of this anabolic steroid hormone or its metabolites were found in any of the samples tested. The method was validated in accordance with the current requirements for confirmatory methods, and the determined parameters of the decision limits necessary for assessing sample compliance met the specified criteria.</p><p><strong>Conclusion: </strong>In 2023, the method was introduced for testing under the National Control Plan in Poland. Up to July 19, 2024, 53 urine samples from different animal species had been tested.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"611-621"},"PeriodicalIF":1.3,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput sequencing as a potential tool in the quality control of infectious bronchitis vaccines.","authors":"Katarzyna Pasik, Katarzyna Domańska-Blicharz, Ewelina Iwan, Arkadiusz Bomba","doi":"10.2478/jvetres-2024-0069","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0069","url":null,"abstract":"<p><strong>Introduction: </strong>In Europe, veterinary vaccines are strictly controlled by the Official Medicines Control Laboratories (OMCLs) of the General European OMCL Network, coordinated by the European Directorate for the Quality of Medicines & HealthCare. Despite a meticulous verification programme for immunological veterinary medicinal products (IVMPs), the products' genomic composition has not yet been subject to evaluation in veterinary pharmacy.</p><p><strong>Material and methods: </strong>A study was carried out on Poland's poultry vaccines containing the infectious bronchitis virus which have the greatest market penetration. Three batches of three different vaccines were high-throughput sequenced and analysed for genomic composition, frequency of variants and the phylogeny of the strains.</p><p><strong>Results: </strong>The main genetic component of each vaccine was infectious bronchitis coronavirus. The identity of the vaccine strain types was confirmed to be consistent with the manufacturer's declaration (793B, Mass and QX). Most variants were identified for the same nucleotide positions in all three batches of each vaccine, demonstrating the homogeneity of the samples, while unique variants specific to single batches were rare.</p><p><strong>Conclusion: </strong>High-throughput sequencing (HTS) is an effective alternative poultry IVMP quality control tool for OMCLs. This technique allows in-depth characterisation of a vaccine strain and assessment of its conformance to the manufacturer's declaration. Importantly, HTS brings new cognitive value to IVMP quality control, because it makes monitoring the level of revertants possible. However, before it could be introduced into routine quality control, a thorough analysis and characteristics of the IVMP to be evaluated is needed from the medical product's manufacturer.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"475-485"},"PeriodicalIF":1.3,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatal <i>Sarcoptes scabiei</i> and <i>Demodex</i> sp. co-infestation in wolves (<i>Canis lupus</i>) at the Białowieża National Park, Poland - is it a consequence of climate change?","authors":"Michał K Krzysiak, Agnieszka Świątalska, Elwira Plis-Kuprianowicz, Andrzej Konieczny, Sławomir Bakier, Krzysztof Tomczuk, Magdalena Larska","doi":"10.2478/jvetres-2024-0068","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0068","url":null,"abstract":"<p><strong>Introduction: </strong>In winter 2021/2022, a wolf population in the primeval Białowieża Forest in Poland was struck by an outbreak of severe mange caused by mixed infestations of <i>Sarcoptes</i> and <i>Demodex</i> mites. We present an epidemiological analysis of this mange which caused significant morbidity and mortality.</p><p><strong>Material and methods: </strong>Ten sites known for wolf activity were monitored by camera trapping. A diagnostic necropsy and testing of a young wolf was performed to determine the causes of death.</p><p><strong>Results: </strong>Five young wolves with severe alopecia of the entire body and some other individuals with minor to medium mange lesions were identified by the camera surveillance. The necropsy of the carcass revealed emaciation, dehydration and anaemia with starvation as the cause of death, likely attributable to severe infestation with <i>Sarcoptes scabiei</i> and <i>Demodex</i> sp. mites. Rabies and infections with <i>Borreliella</i> sp., <i>Anaplasma</i> sp., <i>Ehrlichia</i> sp., <i>Francisella tularensis, Babesia</i> sp. and tick-borne encephalitis virus were excluded by specific tests.</p><p><strong>Conclusions: </strong>The described analysis is the first documented co-infestation of this kind in wolves. The outbreak coincided with very mild winter conditions with a high average minimum temperature, which may have favoured mite survival outside the host, and light snowfall, which may have influenced the wolves' ability to hunt. Other potential drivers of the outbreak could be the large proportion of wetland terrain, increasing number of wolves in the area and anthropogenic pressure on their habitats including the migration crisis at the Polish-Belarusian border and the increased presence of military and border forces, even despite the relief from the anthropogenic pressure from tourism due to the COVID-19 lockdown.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"551-562"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular characterisation of <i>Sarcocystis miescheriana</i> in a pig carcass condemned during routine meat inspection in Poland.","authors":"Weronika Korpysa-Dzirba, Selene Rubiola, Ewa Bilska-Zając, Mirosław Różycki, Francesco Chiesa, Aneta Bełcik, Małgorzata Samorek-Pieróg, Jakub Kubacki, Anna Łukomska, Jacek Karamon, Jacek Sroka, Tomasz Cencek","doi":"10.2478/jvetres-2024-0067","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0067","url":null,"abstract":"<p><strong>Introduction: </strong>This article presents the fourth detection of macroscopic cystic lesions due to sarcocystosis in domestic pigs during routine meat inspection worldwide, and the first molecular detection of <i>Sarcocystis miescheriana</i> in a domestic pig in Poland. Pigs can become intermediate hosts for <i>S. miescheriana</i> by accidental ingestion of oocysts or sporocysts present in food or water contaminated by the faeces of canids (definitive hosts).</p><p><strong>Material and methods: </strong>The affected swine showed no clinical symptoms such as weight loss, dermatitis or dyspnoea suggesting sarcocystosis. The presence of grossly visible cyst-like lesions was noticed by veterinary inspectors during post-mortem meat inspection of pig carcasses at a slaughterhouse located in central Poland. Ten rice-grain-shaped white lesions were isolated from the muscle tissue for molecular analysis, and four other macroscopic cyst-like lesions were also isolated for histopathological and microscopy analysis. The molecular characterisation included amplification and sequencing of the cytochrome C oxidase subunit 1 mitochondrial gene.</p><p><strong>Results: </strong>The cyst-like structures were whitish, calcified, 1 cm long and 3 mm wide. The presence of S. <i>miescheriana</i> DNA was confirmed in all ten grossly visible cyst-like lesions.</p><p><strong>Conclusion: </strong>This study shows that <i>Sarcocystis</i> spp. may be present in swine muscle tissue and cause lesions leading to carcass discard. Further analyses are needed to fully recognise the prevalence and impact of <i>Sarcocystis</i> spp. on animal and human health, especially taking into account the possible presence of the zoonotic <i>S. suihominis</i>.</p>","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":"68 4","pages":"563-569"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}