Journal of Veterinary Research最新文献_第4页

Caesium-137 in the muscles of game animals in 2015-2022 – levels and time trend 2015-2022 年野味动物肌肉中的铯-137 - 含量和时间趋势

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-05-02 DOI: 10.2478/jvetres-2024-0026

Paweł Czerski, M. Gembal, M. Warenik-Bany

{"title":"Caesium-137 in the muscles of game animals in 2015-2022 – levels and time trend","authors":"Paweł Czerski, M. Gembal, M. Warenik-Bany","doi":"10.2478/jvetres-2024-0026","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0026","url":null,"abstract":"\u0000 \u0000 \u0000 Radioactive caesium-137 occurring in the environment may be taken up by plants and animals and pose a trophic threat to humans. Game animals living in forest ecosystems are very good bioindicators of the level of environmental contamination by ionising radiation. The main species measurably exposed to caesium-137 are the wild boar (Sus scrofa), the roe deer (Capreolus capreolus), and the red deer (Cervus elaphus). The study determined the levels of Cs-137 in muscle samples of these game animals in 2015–2022.\u0000 \u0000 \u0000 \u0000 Using gamma radiation spectrometry, 858 samples of game animal muscle tissue were examined: 508 wild boar, 145 roe deer and 205 red deer samples.\u0000 \u0000 \u0000 \u0000 Concentrations of Cs-137 varied widely (from minimum detectable activity (MDA) values to over 4,000 Bq/kg). In 63.4% of cases, the obtained concentrations exceeded the MDA. The permissible limit (600 Bq/kg for food) was exceeded in nine wild boar muscle samples, whereas it was not even exceeded once in roe or red deer muscle. The average concentration in wild boar was three times higher than in roe and red deer and amounted to 42.84 Bq/kg. The highest concentration of Cs-137 in wild boar muscle was 4,195 ± 372.0 Bq/kg, in roe deer muscle it was 111.5 ± 12.50 Bq/kg, and in red deer muscle was 86.70 ± 3.470 Bq/kg.\u0000 \u0000 \u0000 \u0000 The seven years’ data indicate that wild boar absorb the most caesium-137 among game animals. The concentrations of Cs-137 in the muscle of game animals in the years 2015-2022 were at a nearly constant level, a very slow diminution being noticeable over time in roe and red deer muscle.\u0000","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141018690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses. 用于区分小反刍动物慢病毒 A 基因型和 B 基因型菌株的血清学和分子方法的比较。

IF 1.3 3区农林科学

Journal of Veterinary Research Pub Date : 2024-04-30 eCollection Date: 2024-06-01 DOI: 10.2478/jvetres-2024-0025

Monika Olech, Jacek Kuźmak

{"title":"Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses.","authors":"Monika Olech, Jacek Kuźmak","doi":"10.2478/jvetres-2024-0025","DOIUrl":"10.2478/jvetres-2024-0025","url":null,"abstract":"Introduction: Small ruminant lentiviruses (SRLV) cause multisystemic, degenerative and chronic disease in sheep and goats. There are five genotypes (A, B, C, D and E), of which A and B are the most widespread. The purpose of this study was to evaluate the serotyping efficiency of the Eradikit SRLV Genotyping ELISA and the molecular typing efficiency of a newly developed nested real-time PCR targeting the long terminal repeat-gag (LTR-gag) region using samples from animals infected with subtypes of SRLV known to circulate in Poland.Material and methods: A total of 97 sera samples taken from 34 sheep and 63 goats were immunoassayed, and 86 DNA samples from 31 sheep and 55 goats were tested with the PCR. All ruminants were infected with known SRLV strains of the A1, A5, A12, A13, A16, A17, A18, A23, A24, A27, B1 and B2 subtypes.Results: A total of 69 (80.2%, 95% confidence interval 71.6%-88.8%) out of 86 tested samples gave positive results in the PCR. In 17 out of the 86 (19.8%) samples, no proviral DNA of SRLV was detected. The differentiation between MVV (genotype A) and CAEV (genotype B) by PCR matched the predating phylogenetic analysis invariably. No cross-reactivity was observed. On the other hand, the proportion of samples genotyped the same by the older phylogenetic analysis and the Eradikit SRLV Genotyping ELISA was 42.3%. The test was unable to classify 40.2% of samples, and 17.5% of sera were incorrectly classified.Conclusion: Our results showed that the Eradikit SRLV genotyping kit is not a reliable method for predicting SRLV genotype, while the nested real-time PCR based on the LTR-gag region did prove to be, at least for genotypes A and B.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of canine parvovirus occurrence in cats with clinical signs of feline panleukopenia in Slovakia – pilot study 调查斯洛伐克有猫白细胞减少症临床症状的猫体内犬细小病毒的发生情况--试点研究

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-04-15 DOI: 10.2478/jvetres-2024-0021

Alexandra Citarová, Jana Mojžišová, Patrícia Petroušková, Andrea Pelegrinová, Maroš Kostičák, L. Korytár, M. Prokeš, B. Vojtek, A. Ondrejková, M. Drážovská

{"title":"Investigation of canine parvovirus occurrence in cats with clinical signs of feline panleukopenia in Slovakia – pilot study","authors":"Alexandra Citarová, Jana Mojžišová, Patrícia Petroušková, Andrea Pelegrinová, Maroš Kostičák, L. Korytár, M. Prokeš, B. Vojtek, A. Ondrejková, M. Drážovská","doi":"10.2478/jvetres-2024-0021","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0021","url":null,"abstract":"\u0000 \u0000 \u0000 Feline panleukopenia is a contagious viral disease caused by the feline panleukopenia virus (FPV). A closely related pathogen is canine parvovirus (CPV), and amino acid substitutions in this virus allow it to acquire a feline host range. In feline hosts, the disease induced by CPV manifests with similar symptoms to those caused by FPV or milder ones, leading to its underdiagnosis. The aim of this study was to determine the presence of CPV type 2 (CPV-2) in cats with clinical symptoms of panleukopenia and to assess the use of commercial CPV antigen tests for the clinical diagnosis of FPV.\u0000 \u0000 \u0000 \u0000 Samples from 59 cats from central Slovakia were included in the study. Rectal swabs were collected and clinically tested for parvovirus infection using a commercial antigen test. Antigen-positive samples were confirmed by PCR targeting the viral VP2 gene. The sequences of the PCR products were established with the Sanger method.\u0000 \u0000 \u0000 \u0000 Of 59 samples, 23 were revealed to be positive for parvovirus infection by both antigen and PCR test (38.9%). Analysis with the National Center for Biotechnology Information BLASTn application showed 99.78–100% pairwise identity with FPV. The mortality rate of parvovirus-infected cats included in this study was 8.69% (2/23).\u0000 \u0000 \u0000 \u0000 Although feline disease with CPV-2 was not confirmed, the CPV antigen test was able to detect FPV infection.\u0000","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140699750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An evaluation of dogs’ exposure to benzophenones through hair sample analysis 通过毛发样本分析评估狗接触二苯甲酮的情况

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-04-11 DOI: 10.2478/jvetres-2024-0022

S. Gonkowski, Julia Martín, A. Rychlik, I. Aparicio, J. Santos, E. Alonso, Krystyna Makowska

{"title":"An evaluation of dogs’ exposure to benzophenones through hair sample analysis","authors":"S. Gonkowski, Julia Martín, A. Rychlik, I. Aparicio, J. Santos, E. Alonso, Krystyna Makowska","doi":"10.2478/jvetres-2024-0022","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0022","url":null,"abstract":"\u0000 \u0000 \u0000 Benzophenones (BPs) are used in various branches of industry as ultraviolet radiation filters, but they pollute the natural environment, penetrate living organisms, and disrupt endocrine balance. Knowledge of the exposure of domestic animals to these substances is extremely scant. The aim of the study was to investigate long-term exposure of companion dogs to BPs and relate this to environmental factors.\u0000 \u0000 \u0000 \u0000 Hair samples taken from 50 dogs and 50 bitches from under 2 to over 10 years old were analysed for BP content with liquid chromatography–tandem mass spectrometry.\u0000 \u0000 \u0000 \u0000 The results revealed that dogs are most often exposed to 2-hydroxy-4-methoxybenzophenone (BP-3) and 4-dihydroxybenzophenone (BP-1). Concentration levels of BP-3 above the method quantification limit (MQL) were noted in 100% of the samples and fluctuated from 4.75 ng/g to 1,765 ng/g. In turn, concentration levels of BP-1 above the MQL were noted in 37% of the samples and ranged from <0.50 ng/g to 666 ng/g. Various factors (such as the use of hygiene and care products and the dog’s diet) were found to affect BP concentration levels. Higher levels of BP-3 were observed in castrated/spayed animals and in animals that required veterinary intervention more often.\u0000 \u0000 \u0000 \u0000 The results obtained show that the analysis of hair samples may be a useful matrix for biomonitoring BPs in dogs, and that these substances may be toxic to them.\u0000","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140712795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Curcuma longa maceration treatment on ovarian follicular development, serum oestradiol, uterine growth and vascularisation in female albino rats 姜黄浸渍处理对雌性白化大鼠卵泡发育、血清雌二醇、子宫生长和血管形成的影响

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-04-04 DOI: 10.2478/jvetres-2024-0020

Andriyanto Andriyanto, Hamdika Yendri Putra, M. Subangkit, Elpita Tarigan, Leliana Nugrahaning Widi, Yusa Irarang, W. Manalu, Amaq Fadholly

{"title":"Effect of Curcuma longa maceration treatment on ovarian follicular development, serum oestradiol, uterine growth and vascularisation in female albino rats","authors":"Andriyanto Andriyanto, Hamdika Yendri Putra, M. Subangkit, Elpita Tarigan, Leliana Nugrahaning Widi, Yusa Irarang, W. Manalu, Amaq Fadholly","doi":"10.2478/jvetres-2024-0020","DOIUrl":"https://doi.org/10.2478/jvetres-2024-0020","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000 Curcuma longa is a well-known medicinal plant with various health benefits. This study was designed to evaluate the administration of Indonesian C. longa maceration for its effect on promoting growth and development of the ovary and uterus before mating in female albino rats.\u0000 \u0000 \u0000 \u0000 A total of 15 female Sprague Dawley rats in their dioestrous phase were assigned into three different groups: the Control group (mineral water); the Cur-Low group (mineral water with 1% C. longa maceration) and the Cur-High group (mineral water with 5% C. longa maceration). The treatments were given for 20 days. Serum concentrations of follicle-stimulating hormone, oestradiol and progesterone were determined. After the sacrifice of the rats, ovary and uterine relative weight, uterine cornua diameter and length, uterine gland diameter (by histology), the number of primary, secondary, tertiary, and Graafian follicles, the number of corpora lutea and vascular endothelial growth factor (VEGF) expression in the ovary were measured. Uterine vascularisation was also evaluated.\u0000 \u0000 \u0000 \u0000 Administration of C. longa maceration significantly improved the relative weights of the uterus and ovary; uterine cornua diameter, length and vascularisation; uterine gland diameter; and expression of VEGF in the ovary. It also increased the number of tertiary follicles and corpora lutea, albeit not significantly. Follicle-stimulating hormone serum concentrations were lower in the administered rats.\u0000 \u0000 \u0000 \u0000 Oestradiol and progesterone levels rose with C. longa maceration treatment. The maceration improved the reproductive organs of unmated rats and had potential to optimise the uterine environment for supporting pregnancy in order to produce high-quality offspring.\u0000","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140742128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analysis of the trypanosomatid parasite Lotmaria passim in honey bees (Apis mellifera) in Poland. 波兰蜜蜂（Apis mellifera）中锥虫寄生虫 Lotmaria passim 的系统发育分析。

IF 1.3 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0018

Maria Iller, Karolina Lipczyńska-Ilczuk, Rajmund Sokół, Grzegorz Borsuk, Agata Bancerz-Kisiel

{"title":"Phylogenetic analysis of the trypanosomatid parasite Lotmaria passim in honey bees (Apis mellifera) in Poland.","authors":"Maria Iller, Karolina Lipczyńska-Ilczuk, Rajmund Sokół, Grzegorz Borsuk, Agata Bancerz-Kisiel","doi":"10.2478/jvetres-2024-0018","DOIUrl":"10.2478/jvetres-2024-0018","url":null,"abstract":"Introduction: Lotmaria passim (L. passim) is a single-celled flagellate which colonises the bee gastrointestinal tract and is highly prevalent in honey bees. This parasite is associated with colony losses. Honey bee (Apis mellifera) colonies were sampled from five apiaries in the north-eastern part of Poland for the phylogenetic analysis of L. passim.Material and methods: Each apiary consisted of approximately 60 bee colonies, of which 20 were randomly selected. Samples of 60 differently aged worker bees were collected from each colony and pooled. A total of 100 bee colonies from five apiaries were examined. Protozoa of the Trypanosomatidae family were identified by PCR. L. passim was detected in 47 (47%) of the samples. The 18S ribosomal (r) RNA amplicons of L. passim were sequenced by a commercial service. Their sequences were analysed with BLASTN and noted to be compatible with the GenBank sequences of this region of the organism's genome. A sequence analysis was performed using the BioEdit Sequence Alignment Editor and Clustal W software.Results: The amplicon sequences of L. passim were 100% homologous with the sequences deposited in GenBank under accession numbers KM066243.1., KJ684964.1 and KM980181.1.Conclusion: This is the first study to perform a phylogenetic analysis of L. passim in Polish honey bees. The analysis demonstrated high levels of genetic similarity between isolates of L. passim colonising apiaries in the north-eastern region of Poland.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first confirmed cases of pigeon rotavirus A (RVA) infection in domestic pigeons (Columba livia) in Poland. 波兰首例家鸽轮状病毒 A (RVA) 感染确诊病例。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0006

Krzysztof Adamczyk, Dennis Rubbenstroth, Aleksandra Ledwoń, Rafał Sapierzyński, Piotr Szeleszczuk

{"title":"The first confirmed cases of pigeon rotavirus A (RVA) infection in domestic pigeons (Columba livia) in Poland.","authors":"Krzysztof Adamczyk, Dennis Rubbenstroth, Aleksandra Ledwoń, Rafał Sapierzyński, Piotr Szeleszczuk","doi":"10.2478/jvetres-2024-0006","DOIUrl":"10.2478/jvetres-2024-0006","url":null,"abstract":"Introduction: Although the presence of rotaviruses in pigeon samples has been reported since the 1980s, its importance as an aetiological agent of the \"classical\" young pigeon disease (YPD) was not proven until 2020, when the Henle-Koch postulates were confirmed for pigeon-type rotavirus A (RVA) genotype G18P(17).Material and methods: From 2011 to 2020, archived liver samples from 117 pigeons submitted by 74 individual lofts were tested for the presence of pigeon-type RVA using a VP6-specific RT-qPCR test. For four positive racing pigeons, a more detailed necropsy and histopathological analysis was performed.Results: Indicators of an acute RVA infection were found in 24 out of 117 (20.5%) samples tested, the earliest in 2014. Necropsies of the four selected RVA-positive pigeons showed changes mainly in the liver, spleen and kidneys similar to those described by other researchers. The histopathological examination revealed mainly hyperaemia and necrosis in the liver, as well as mononuclear cell infiltrates in these organs.Conclusion: Pigeon-type RVA is also a cause of YPD in Poland and is a serious challenge for racing pigeon breeders and veterinarians, especially during the training and flights of young pigeons.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue expression of porcine transient receptor potential mucolipin protein channels and their differential responses to porcine reproductive and respiratory syndrome virus infection in vitro. 猪瞬时受体电位粘脂蛋白通道的组织表达及其对猪繁殖与呼吸综合征病毒体外感染的不同反应。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0014

Zhiqiang Xia, Denggao Long, Xinyi Hong, Ying Lan, Lixia Xie

{"title":"Tissue expression of porcine transient receptor potential mucolipin protein channels and their differential responses to porcine reproductive and respiratory syndrome virus infection in vitro.","authors":"Zhiqiang Xia, Denggao Long, Xinyi Hong, Ying Lan, Lixia Xie","doi":"10.2478/jvetres-2024-0014","DOIUrl":"10.2478/jvetres-2024-0014","url":null,"abstract":"Introduction: Porcine reproductive and respiratory syndrome virus (PRRSV) infection results in a serious disease, posing a huge economic threat to the global swine industry. The transient receptor potential mucolipin proteins (TRPMLs) have been shown to be strongly associated with virus infection and other physiological processes in humans, but their tissue distribution and responses to PRRSV in pigs remain unknown.Material and methods: Quantitative reverse-transcription PCR analysis was undertaken to determine the optimal primer for TRPML expression detection and for quantifying that expression individually in different pig tissue samples. Meat Animal Research Center 145 (MARC-145) monkey kidney cells and the TRPML-specific activator mucolipin synthetic agonist 1 (ML-SA1) were used to reveal the relationship between TRPML and PRRSV-2 infection.Results: The best primers for each TRPML gene used in a fluorescence-based quantitative method were identified and TRPML1 was found to be highly expressed in the kidneys and liver of pigs, while TRPML2 and TRPML3 were observed to be primarily expressed in the kidney and spleen tissues. The expression of TRPML2 was upregulated with the rise in PRRSV-2 infection titre but not the expression of TRPML1 or TRPML3, and ML-SA1 inhibited PRRSV-2 in a dose-dependent manner.Conclusion: Our research revealed the gene expression of TRPMLs in pigs and identified that TRPML channels may act as key host factors against PRRSV infection, which could lead to new targets for the prevention and treatment of pig infectious diseases.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrine and icariin can inhibit bovine viral diarrhoea virus replication by promoting type I interferon response in vitro. 马钱子碱和冰片苷可通过促进体外 I 型干扰素反应来抑制牛病毒性腹泻病毒的复制。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0013

Dongjie Cai, Zifan Shen, Bin Tian, Jie Chen, Yilin Zhang, Liuhong Shen, Ya Wang, Xiaoping Ma, Zhicai Zuo

{"title":"Matrine and icariin can inhibit bovine viral diarrhoea virus replication by promoting type I interferon response in vitro.","authors":"Dongjie Cai, Zifan Shen, Bin Tian, Jie Chen, Yilin Zhang, Liuhong Shen, Ya Wang, Xiaoping Ma, Zhicai Zuo","doi":"10.2478/jvetres-2024-0013","DOIUrl":"10.2478/jvetres-2024-0013","url":null,"abstract":"Introduction: Bovine viral diarrhoea virus (BVDV) can cause diarrhoea (BVD) in an animal herd, leading to heavy economic losses. There are limited drugs available for treating and controlling BVD. This research aims to investigate the antiviral and immunoregulatory effects of two traditional Chinese herb extracts against BVDV infection. The extracts are matrine and icariin, which have been proved to have immunostimulant and antiviral effects.Material and methods: A cell counting kit-8 assay was used to analyse the toxicity of matrine and icariin to Madin-Darby bovine kidney (MDBK) cells. The model of MDBK cells infected with BVDV was utilised to uncover the antiviral mechanism of matrine and icariin, which along with their immunoregulatory ability was evaluated by quantitative reverse-transcription PCR and ELISA.Results: The results showed that matrine and icariin can significantly inhibit the gene expression level of the BVDV 5' untranslated region through various pathways. Both matrine and icariin can statistically upregulate the gene expression level of interferon alpha, interferon beta (IFN-β), toll-like receptor 3, retinoic acid-inducible gene I and interferon regulatory factor 3, and raise the concentration of IFN-β after BVDV infection.Conclusion: This study proves that both matrine and icariin have inhibitory effects on BVDV replication by activating IFN production and the IFN signalling pathway. The finding is promising and should open up the possibility of larger-scale in vitro research followed by in vivo experiments evaluating matrine and icariin as therapeutic agents in BVD cases.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon. 富血小板和贫血小板浓缩物刺激后单核细胞衍生巨噬细胞的活性。对掺硅类金刚石碳涂层胫骨植入物插入绵羊模型的研究。

IF 1.8 3区农林科学

Journal of Veterinary Research Pub Date : 2024-03-23 eCollection Date: 2024-03-01 DOI: 10.2478/jvetres-2024-0003

Bartłomiej Szymczak, Andrzej Junkuszew, Krzysztof Patkowski, Tomasz Szponder, Dominika Nguyen Ngoc, Beata Drzewiecka, Aleksandra Sobczyńska-Rak, Joanna Wessely-Szponder

{"title":"The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon.","authors":"Bartłomiej Szymczak, Andrzej Junkuszew, Krzysztof Patkowski, Tomasz Szponder, Dominika Nguyen Ngoc, Beata Drzewiecka, Aleksandra Sobczyńska-Rak, Joanna Wessely-Szponder","doi":"10.2478/jvetres-2024-0003","DOIUrl":"10.2478/jvetres-2024-0003","url":null,"abstract":"Introduction: Macrophages are crucial immune cells that play a role in tissue repair and can exhibit pro- or anti-inflammatory behaviour based on environmental stimulation. Their functional phenotype can be affected by platelet-derived products as determined by those products' composition. When the inflammatory response caused by implantation is excessive, it can lead to rejection of the implant. Therefore, a thorough evaluation of implant haemocompatibility is necessary to minimise undesirable consequences.Material and methods: In an in vitro study, monocyte-derived macrophages (MDMs) were obtained from the whole blood of sheep after a silicon-doped diamond-like carbon-coated implant insertion. These MDMs were then exposed to autologous platelet-derived products for functional marker analysis.Results: Platelet-poor plasma (PPP) and pure platelet-rich plasma (P-PRP) stimulation increased arginase-1 activity, while leukocyte-rich PRP stimulation produced a mixed response involving higher O2- (6.49 ± 2.43 nM vs non-stimulated 3.51 ± 1.23 nM, P-value < 0.05) and NO (3.28 ± 1.38 μM vs non-stimulated 2.55 ± 0.32μM, P-value < 0.05) generation.Conclusion: Using PPP and P-PRP stimulation in post-implantation procedures may contribute to the polarisation of macrophages towards the M2-like pro-resolving phenotype, thereby accelerating wound healing. This would also prevent implant degradation due to an excessive inflammatory process.","PeriodicalId":17617,"journal":{"name":"Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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