Journal of water process engineering最新文献

{"title":"Treatment of food processing wastewaters by using Black Soldier Fly larvae: Preliminary results","authors":"Valentina Grossule,&nbsp;Mia Henjak,&nbsp;Raffaello Cossu","doi":"10.1016/j.jwpe.2025.107746","DOIUrl":"10.1016/j.jwpe.2025.107746","url":null,"abstract":"<div><div>The biological process based on the use of Black Soldier Fly larvae (BSFL) has emerged as an innovative treatment technology for high organic content (HOC) wastewaters (WW), such as those from food and beverage sector. The process offers both higher treatment efficiency and generation of larval biomass for resource recovery. This study provides the first insight into the suitability of real food-processing HOC-WWs to the BSFL treatment process. Six food-processing wastewaters were tested, such as from bakery, brewery, dairy, juice production, slaughterhouse, and winery. Preliminary evaluation of WW suitability was based on WW quality in terms of organic content, nitrogen, phosphorous and dominant macronutrients for larvae diet (proteins, carbohydrates, and lipids). The BSFL process performance was evaluated by monitoring larval development and removal of organic matter, nitrogen and phosphorous. Bakery, brewery and winery showed to be suitable for BSFL process, being characterised by high TOC concentrations and by good nutritional quality, achieving up to 93 %, 86 % and 95 % removal of TOC, COD and BOD respectively. This confirmed BSF process as an efficient technology for organics removal and bioconversion into larval biomass In contrast, dairy, juice, slaughterhouse samples, showed to not be suitable as single feeding. Nutritional quality can be improved by appropriate mixing of various type of WW.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107746"},"PeriodicalIF":6.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effective removal of Sb(V) by bio-electrochemical systems: Performance and mechanism","authors":"Mengqiao Luo ,&nbsp;Lei Tang ,&nbsp;Lei Fang ,&nbsp;Yongchao Zhou ,&nbsp;Yiping Zhang","doi":"10.1016/j.jwpe.2025.107742","DOIUrl":"10.1016/j.jwpe.2025.107742","url":null,"abstract":"<div><div>Recently, Antimony (Sb) pollution in wastewater is a global environmental issue. The bio-electrochemical system (BES) converts Sb(V) to less toxic Sb(III) via microbial electrochemical processes. In this study, the effect factors of Sb(V) removal by BES are explored and the removal mechanism by BES is clarified. Results show that under acidic conditions, the removal rate of Sb(V) was 100 %, which is far more than its removal efficiency under a neutral condition. Sulfide concentration critically regulates efficiency: levels &gt;8.5 mg L⁻¹ inhibit removal, while &lt;6 mg L⁻¹ promote it via H₂SbS₄⁻ formation, linked to sulfate-reducing bacteria (SRB) metabolizing S²⁻. Furthermore, the mechanism of bio-electrochemical system (BES) reactor was further clarified by 16 s rRNA high-throughput sequencing. There are three types of bacteria play an important role in the removal of Sb from BES. ARB can reduce directly Sb(V) to sediment (i.e. Sb₂O₃, Sb(OH)₃) in BES, respectively. Electrochemically active bacteria (EAB) can transfer electrons to Sb(V) in solution by graphite electrodes and promote the reduction in BES. And SRB can use organic matter as electron donor to reduce SO₄²⁻ to S²⁻ in respiratory metabolism process. Electric fields can stimulate parts of microbial activities, so that it can improve the removal efficiency of Sb(V). Sb of the wastewater are removed by antimony-reducing bacteria, sulfide reduction, and electrochemistry combined with microbial reduction. Therefore, BES have a good performance in the removal of Sb(V) in acidic wastewater. This study presents a novel method for treating Sb(V), which is more sustainable compared to traditional chemical approaches.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107742"},"PeriodicalIF":6.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced degradation of rhodamine B by UV/Co3O4@BC/PMS system: Performance and mechanism","authors":"Cuizhen Sun ,&nbsp;Mengyao Li ,&nbsp;Qiuxiang Wang ,&nbsp;Rupeng Liu ,&nbsp;Menghan Li ,&nbsp;Feiyong Chen ,&nbsp;Sisi Xu ,&nbsp;Guoxiu Wang","doi":"10.1016/j.jwpe.2025.107719","DOIUrl":"10.1016/j.jwpe.2025.107719","url":null,"abstract":"<div><div>The degradation of rhodamine B (RhB), a commonly used dye in the textile industry, is a concern in dye wastewater treatment. Advanced oxidation technology using Peroxymonosulfate (PMS) shows promise due to its highly asymmetry molecular structure. To enhance RhB removal efficiency, a novel catalyst, called Co₃O₄@BC, was developed. This catalyst consists of cobalt oxide (Co₃O₄) loaded onto biochar (BC). Additionally, a UV/Co₃O₄@BC/PMS system was developed. The photocatalytic degradation performance of RhB by this system was investigated. With the addition of only 20 mg·L⁻¹ of Co₃O₄@BC, a degradation rate of 99.8 % for RhB was achieved in the system within 25 min, a 56 % improvement compared to the system without Co₃O₄@BC. Additionally, when the concentration of peroxymonosulfate (PMS) was increased from 0.02 g·L⁻¹ to 0.4 g·L⁻¹, the degradation rate increased by 42.5 %. As the concentration of Co₃O₄@BC increased from 5 mg·L⁻¹ to 20 mg·L⁻¹, the degradation rate improved by 15.6 %. When the UV radiation intensity increased from 10 W·m⁻² to 120 W·m⁻², the degradation rate increased by only 3.3 %. It was found that Co₃O₄@BC had an excellent ability to activate PMS to generate active species, with the contribution of reactive oxygen species in the order of ¹O₂&gt;SO₄^•-&gt;•OH. The leaching concentration of cobalt ions in UV/Co₃O₄@BC/PMS system was 121.1 μg·L⁻¹, which still meets the specified limit by the Environmental Quality Standard for Surface Water (Gb3838-2022). It well below the specified limit of 1.0 mg·L⁻¹. Therefore, the use of the UV/Co₃O₄@BC/PMS system is practical and economically feasible from both economic and environmental perspectives.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107719"},"PeriodicalIF":6.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of physical treatments and moisture content on chitosan-based hydrogels designed to adsorb ethinylestradiol at low concentrations","authors":"Trevor Bell ,&nbsp;Jason Robert Tavares ,&nbsp;Marie-Josée Dumont","doi":"10.1016/j.jwpe.2025.107709","DOIUrl":"10.1016/j.jwpe.2025.107709","url":null,"abstract":"<div><div>Anthropogenic impacts on the environment have increased research regarding environmental pollution and water quality. Studies reporting emerging contaminants have raised concerns as their effects on health and ecosystems are unknown. Hormonally active agents are a prominent subcategories of emerging contaminant pollution consisting of natural and synthetic compounds. Ethinylestradiol is a synthetic pharmaceutical steroid hormone of interest because of its mechanisms of entering the environment and its metabolic resistance. Adsorption technologies are favourable methods of pollutant removal due to their relative ease of implementation, cost-effectiveness, and potential for environmental friendliness. Hydrogels are three-dimensional hydrophilic networks of chain polymers that are well documented for their adsorption capabilities. Chitosan hydrogels were used to adsorb ethinylestradiol, and crosslinking was performed with glutaraldehyde to increase the hydrophobicity of the matrix. Historically, materials designed to adsorb ethinylestradiol have been tested at high concentrations which are unrealistic of what is measured in the environment, thus low concentrations of ethinylestradiol were used in adsorption testing. Physical treatment methods including microwave treatment, liquid nitrogen submersion, and overnight freezing were explored to increase the pore size and exposed surface area of the hydrogels. Treatments performed after synthesis increased the adsorption capacity of the hydrogels, particularly when the moisture content was high. Microwave-treated hydrogels adsorbed the most ethinylestradiol with an adsorption efficiency of 62 % when tested at a low ethinylestradiol concentration (400 ng/mL). High moisture content liquid nitrogen-treated hydrogels and microwave treated hydrogels adsorbed the most ethinylestradiol when tested at higher concentrations (5000 ng/mL), resulting in a removal efficiency of 61 %.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107709"},"PeriodicalIF":6.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable water treatment: Synthesis and characterization of g-C3N5/NiCo2S4 heterojunction nanocomposite for efficient visible light-induced degradation of highly hazardous organic pollutants","authors":"Sathiya Balashanmuga Nehru,&nbsp;Nagapandiselvi Perumal,&nbsp;Yazhini Priya Subbarayalu","doi":"10.1016/j.jwpe.2025.107712","DOIUrl":"10.1016/j.jwpe.2025.107712","url":null,"abstract":"<div><div>Recent modernization has fostered economic growth and altered lifestyles, but has also increased environmental pollution, particularly water contamination. In addressing this challenge, photocatalysis utilizing various photocatalysts, particularly graphitic carbon nitride (g-C₃N₄), has gained significant attention. Building on the success of g-C₃N₄, g-C₃N₅ has emerged as a promising photocatalyst with enhanced properties. In this study, a g-C₃N₅/NiCo₂S₄ nanocomposite was synthesized by varying the concentrations of g-C₃N₅ and NiCo₂S₄. The structural characteristics and their variations were analysed using XRD and FT-IR analyses. The chemical coordination and optical properties were investigated by XPS and UV–Vis spectroscopy, respectively. Pristine g-C₃N₅ exhibits an agglomerated sheet-like structure, whereas NiCo₂S₄ has a bulk, plate-like morphology. In the nanocomposites, the NiCo₂S₄ plate-like structures are uniformly distributed on the surface of g-C₃N₅ sheets, as observed in SEM and HR-TEM, and EDAX analysis confirms the presence of all elements in the composite. The photocatalytic degradation efficiency of the nanocomposites was evaluated using Brilliant Green (BG) dye and Congo Red (CR) dye under visible light irradiation and the results were compared with pristine materials. The degradation efficiency improved with increasing g-C₃N₅ loading, achieving 94.37 % degradation of BG dye within 100 min and 88.83 % degradation of CR dye within 180 min. The nanocomposite showed excellent stability over five cycles, making it a viable and cost-effective candidate with good efficiency. Thus, this nanocomposite holds significant promise for the sustainable degradation of toxic organic pollutants, offering a potential solution in future wastewater treatment.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107712"},"PeriodicalIF":6.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indigenous fungi derived from a municipal wastewater treatment plant with rapid flocculation of Chlorella sp.: Convenience, mechanisms, and conditions optimization","authors":"Ruxian Jing,&nbsp;Xiuhong Liu,&nbsp;Qing Yang,&nbsp;yaxin Wang,&nbsp;Nan Zhang,&nbsp;Shiyong Zhang","doi":"10.1016/j.jwpe.2025.107693","DOIUrl":"10.1016/j.jwpe.2025.107693","url":null,"abstract":"<div><div>Few indigenous fungi species that can flocculate microalgae in wastewater treatment plants (WWTPs) have been reported, and more effective fungi strains for microalgal flocculation remain to be identified. In this research, a flocculating fungi strain was screened from a WWTP. The flocculation performance and properties were assessed. The underlying mechanisms were elucidated through extracellular polymeric substances (EPS) analysis. The flocculation conditions were optimized by the response surface method (RSM). The findings indicated that the isolated strain <em>Curvularia moringae</em> J-26 flocculated <em>Chlorella</em> sp. with an efficiency of up to 97.3 % within 120 min. <em>Curvularia moringae</em> J-26 flocculated <em>Chlorella</em> sp. mainly via mycelium absorption, attributed to the protein-like (PN) and soluble microbial byproduct-like substances contained in the EPS. The optimal conditions to flocculate <em>Chlorella</em> sp. were the mycelium pellets dosage of 2.25 g/L at 160 rpm for 4.8 h. <em>Curvularia moringae</em> J-26 flocculated <em>Chlorella</em> sp. with good performance, which had the potential to reduce the cost of <em>Chlorella</em> sp. harvesting. This research provided a technical reference for <em>Chlorella</em> sp. harvesting and fungi application in wastewater treatment.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107693"},"PeriodicalIF":6.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxyl-regulated ZnFe2O4/UV/PDS synergy for green conversion of NH4+-N: Insights from experiment and theory","authors":"Youwei Yang ,&nbsp;Jianxing Wang ,&nbsp;Yan Gao ,&nbsp;Xuekun Tang ,&nbsp;Chunying Wang ,&nbsp;Xianping Luo","doi":"10.1016/j.jwpe.2025.107729","DOIUrl":"10.1016/j.jwpe.2025.107729","url":null,"abstract":"<div><div>ZnFe₂O₄ catalysts synthesized via hydrothermal (ZFO-H) and sol-gel (ZFO-S) methods were employed to activate peroxodisulfate (PDS) under UV light for the efficient conversion of low-concentration NH₄⁺-N into environmentally benign N₂, offering a promising solution for advanced wastewater treatment. Characterization revealed that the sol-gel-derived ZFO-S exhibited uniform agglomeration, smooth surfaces, and an 80% reduction in surface hydroxyl density (as indicated by the FTIR peak area at 3320 cm^-1), outperforming both hydrothermally prepared and commercial ZnFe₂O₄ (ZFO-H and ZFO-M, respectively). This study addresses the critical challenge of ammonia nitrogen removal in wastewater, where conventional methods often struggle with low efficiency or secondary pollution. Mechanistic studies demonstrated that ZnFe₂O₄ facilitated electron transfer at Zn sites to activate PDS, generating reactive species dominated by singlet oxygen (¹O₂), which drove NH₄⁺-N oxidation. Density functional theory (DFT) calculations further elucidated the reaction pathway, highlighting preferential adsorption of NH₃ at oxygen sites on the ZnFe₂O₄ (311) surface, followed by sequential dehydrogenation and coupling to form N₂. Under optimal conditions (pH &gt;9.25, 10 mM PDS, 25°C, 3 h), 97.40% of initial 50 mg/L NH₄⁺-N was converted to N₂, with negligible NO₃⁻ byproduct formation (&lt; 6%), demonstrating high feasibility for practical wastewater remediation. The reduced surface hydroxyl groups and lower work function of ZFO-S enhanced electron transfer, underscoring its superior catalytic activity. This work provides a novel strategy for designing hydroxyl-regulated photocatalysts tailored for wastewater treatment and advances the mechanistic understanding of persulfate-based ammonia nitrogen remediation in engineered water systems.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107729"},"PeriodicalIF":6.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The function of carbon quantum dots (CQDs) derived from peapod biomass in composite photocatalysts for the enhanced photodegradation of perfluoroalkyl carboxylic acids (PFCAs) under UVC and visible light irradiation","authors":"Mona Nejatpour ,&nbsp;Bergüzar Yılmaz ,&nbsp;Burcu Ozden ,&nbsp;Sibel Barisci ,&nbsp;Meral Dükkancı","doi":"10.1016/j.jwpe.2025.107750","DOIUrl":"10.1016/j.jwpe.2025.107750","url":null,"abstract":"<div><div>The remediation of perfluoroalkyl carboxylic acids (PFCAs) presents a significant challenge due to their environmental persistence and detrimental impact. In this study, carbon quantum dots (CQDs) were sustainably prepared using peapod biomass via the hydrothermal method and incorporated into a titanium dioxide (TiO₂) composite photocatalyst. Chemical, morphological, and optical analysis confirmed the efficient synthesis of the CQD/TiO₂ composite, which exhibited enhanced optical properties. Photocatalytic oxidation experiments demonstrated that the CQD/TiO₂ composite achieved significantly higher degradation efficiencies for perfluorooctanoic acid (PFOA-C₈) compared to pristine TiO₂, with 26.3 % and 24.5 % improvements when irradiated under Ultraviolet-C (UVC) and visible light, respectively. Furthermore, the photocatalytic oxidation of short-chain PFCAs, specifically perfluorohexanoic acid (PFHxA-C₆), perfluoropentanoic acid (PFPeA-C₅), perfluorobutanoic acid (PFBA-C₄), and perfluoropropanoic acid (PFPrA-C₃), were evaluated under visible light irradiation. Degradation rates for PFHxA-C₆ and PFPeA-C₅ significantly enhanced from 14.6 % and 19.6 % to 41.3 % and 52.1 %, respectively, when using CQD-coupled TiO₂ (CQD/TiO₂) compared to pure TiO₂. The first-order rate constants revealed accelerated PFCA degradation using PPCQD/TiO₂ photocatalysts. Intermediate formation (C₃–C₆) and defluorination analyses revealed stepwise decomposition of PFCAs, with enhanced CF₂ detachment in the presence of the CQD/TiO₂ composite. The role of scavengers in PFOA degradation under visible light was also examined. This research underscores the potential of CQD-enhanced TiO₂ photocatalysts for effective PFCA remediation under sustainable conditions.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107750"},"PeriodicalIF":6.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upcycling Guiera Senegalensis waste into biochar for sustainable amoxicillin removal from water","authors":"Cheikh Gaye ,&nbsp;Jorge Rodríguez-Chueca ,&nbsp;Alba Ortíz Fuduo ,&nbsp;Modou Fall ,&nbsp;Patricia García-Muñoz","doi":"10.1016/j.jwpe.2025.107726","DOIUrl":"10.1016/j.jwpe.2025.107726","url":null,"abstract":"<div><div>The increasing environmental pollution caused by antibiotics and solid waste presents a dual challenge. Biomass-based remediation strategies offer a promising approach to enhancing the circular economy, yet they remain a significant research challenge. In this study, a biochar with high adsorption capacity and catalytic activity was synthesized from agricultural waste (<em>Guiera senegalensis</em>) for the first time. The resulting biochar (GSBC) was applied in the adsorption and degradation of amoxicillin (AMX) through an advanced oxidation process (AOP) based on peroxymonosulfate (PMS) activation. The combined process (0.9 g/L GSBC/0.01 mM PMS) achieved AMX degradation rates of approximately 95.0 % and 90.0 % within 45 min in distilled and river water, respectively. Mechanistic studies using radical scavengers revealed that AMX removal occurred via both radical (HO^•, SO₄^{• –}and O₂^{• –}) and non-radical (¹O₂) pathway. The influence of inorganic salts (Cl⁻, NO₃⁻, CO₃²⁻, SO₄²⁻and PO₄³⁻) was also evaluated, with SO₄²⁻and PO₄³⁻ exerting the greatest inhibitory effects; however, removal efficiency remained around 80 % despite their presence. The GSBC/PMS system was further tested in various real water matrices, including river water, fountain tower water, aquaculture facility water, and tap water, demonstrating comparable efficiency to that observed in pure water, particularly in river water. Phytotoxicity assessments in different matrices also indicated better performance in river water. This study introduces a novel biochar precursor, promotes agricultural waste valorization, and contributes to the development of sustainable wastewater treatment within the framework of the circular economy.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107726"},"PeriodicalIF":6.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking virulence factor genes and their combination with resistance genes for microbial risk assessment in drinking water treatment plants","authors":"Qingqing Zhao ,&nbsp;Xuebing Pan ,&nbsp;Tianhang Gu ,&nbsp;Yali Song ,&nbsp;Minjie Zhou ,&nbsp;Xiangdong Xue ,&nbsp;Bingzhi Dong","doi":"10.1016/j.jwpe.2025.107741","DOIUrl":"10.1016/j.jwpe.2025.107741","url":null,"abstract":"<div><div>Virulence factor genes (VFGs) and their intragenomic combinations with antibiotic resistance genes (ARGs) have been recognized as important targets for microbiological health risk assessment. Yet, their dynamics during drinking water treatment plants (DWTPs) remain unknown. Herein, we conducted an advanced VFG-centric metagenomic strategy to track the fate of VFGs and the intragenomic combination of VFG and ARG during two full-scale DWTPs. We detected 197 VFGs belonging to 12 categories in the two DWTPs by metagenomic sequencing. Both DWTPs elevated the relative abundance of total VFGs, revealing the potential microbial health risks during drinking water treatment. The post-ozonation unit could promote the relative abundance of 8 dominant VFG categories (0.09–3.02-log₁₀). We identified 59 intragenomic VFG-ARG combinations (including 17 combinations of VFG and multiple ARGs) via metagenomic assembly. The DWTP adopting ozone-biological activated carbon treatment processes was revealed to induce the integration of VFG and ARG (even multiple ARGs), which might promote the microbial health risk for effluent. <em>Mycobacterium</em>, <em>Acidovorax</em>, <em>Pseudomonas</em>, and <em>Novosphingobium</em> genera were identified as the primary hosts for VFGs and crucial factors affecting the VFG dynamics during two DWTPs. This research provides new insights into the assessment and control of microbial health risks in drinking water treatment.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"73 ","pages":"Article 107741"},"PeriodicalIF":6.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}