C.A. Dykes , J. Pearson , G.D. Bending , S. Abolfathi
{"title":"Spatial and temporal dynamics of methane and carbon dioxide fluxes in a constructed wetland","authors":"C.A. Dykes , J. Pearson , G.D. Bending , S. Abolfathi","doi":"10.1016/j.jwpe.2025.108130","DOIUrl":"10.1016/j.jwpe.2025.108130","url":null,"abstract":"<div><div>Constructed wetlands (CWs) are increasingly adopted as a nature-based solution for wastewater treatment. However, given their affinity with natural wetlands and high degradation of organic pollutants, their classification as a sustainable ‘green’ technology is questioned due to potentially high greenhouse gas (GHG) emissions. While methane (CH<sub>4</sub>) and carbon dioxide (CO<sub>2</sub>) emissions from natural wetlands and ponds have been widely studied, fluxes from CWs remain underexplored. To address this knowledge gap, CH<sub>4</sub> and CO<sub>2</sub> fluxes were measured from the Ingoldisthorpe integrated CW and an adjacent natural pond, located in the UK using the floating chamber method with gas chromatography, alongside climate and water quality monitoring. Mean seasonal CH<sub>4</sub> fluxes ranged from 0.13 ± 0.18 mg CH<sub>4</sub> m<sup>−2</sup> h<sup>−1</sup> (autumn) to 20.52 ± 45.01 mg CH<sub>4</sub> m<sup>−2</sup> h<sup>−1</sup> (spring), with ebullition contributing 25–93 % of total spring-summer CH<sub>4</sub> flux. When CH<sub>4</sub> was converted to CO<sub>2</sub> equivalents, the CH<sub>4</sub>:CO<sub>2</sub> seasonal ratio revealed a greater contribution of CH<sub>4</sub> to the CW emission potential in spring-summer and CO<sub>2</sub> (mg CO<sub>2</sub> m<sup>−2</sup> h<sup>−1</sup>) in autumn-winter, resulting in a mean annual global warming potential (GWP) of 3.76 kg CO<sub>2</sub>-eq m<sup>−2</sup> year<sup>−1</sup>. Temporal temperature variability significantly influenced CH<sub>4</sub> ebullitive fluxes, while spatial variability of water depth and velocity were key drivers of CH<sub>4</sub> and CO<sub>2</sub> diffusive fluxes. The absence of ebullitive flux in the natural pond adjacent to the CWs was attributed to its lower eutrophic conditions than the CWs. However, no significant differences in overall GHG emissions were observed between the CWs and the natural pond. Overall, our findings capture the complex temporal and spatial variability of GHG fluxes in CWs, highlighting the importance of comprehensive sampling to inform more accurate GHG budgeting and support evidence-based design and management strategies for sustainable CW systems.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108130"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luz A. Rincón-Barón , Luis A. Cañón-Tafur , Catalina Gómez-Prias , Mayra A. Garzón-González , Camila Gracia-Sánchez , Camilo Quiñonez-Casallas , Luis C. Jiménez-Borrego , Lucia A. Díaz-Ariza , Augusto E. Mejía-Gómez , Camilo Velez , Ana K. Carrascal-Camacho , Claudia M. Rivera-Hoyos , Aura M. Pedroza-Rodríguez
{"title":"Pilot plant use of two types of TiO2 thin-films as a sustainable alternative for reuse of kitchen greywater","authors":"Luz A. Rincón-Barón , Luis A. Cañón-Tafur , Catalina Gómez-Prias , Mayra A. Garzón-González , Camila Gracia-Sánchez , Camilo Quiñonez-Casallas , Luis C. Jiménez-Borrego , Lucia A. Díaz-Ariza , Augusto E. Mejía-Gómez , Camilo Velez , Ana K. Carrascal-Camacho , Claudia M. Rivera-Hoyos , Aura M. Pedroza-Rodríguez","doi":"10.1016/j.jwpe.2025.108100","DOIUrl":"10.1016/j.jwpe.2025.108100","url":null,"abstract":"<div><div>The accelerated growth of the world's population generates large volumes of domestic wastewater, therefore the objective of this research was to perform the photocatalytic treatment and reuse of kitchen greywater by using a laboratory scale and pilot plant, TiO<sub>2</sub> films, deposited on commercial glass substrates and laminar biochar of Guadua, through peroxo sol-gel (PSG), atomic layer deposition (ALD) and magnetron sputtering (MS) methods; laboratory-scale tests involved films deposited by peroxo sol-gel (PSG), atomic layer depositions (ALD), and magnetron sputtering (MS); later the two less expensive with best results were evaluated in the pilot plant, composed of a homogenisation tank, a filtration system and the photocatalysis reactor. Laboratory results showed that all three kinds of TiO<sub>2</sub> films inactivated >70 % of <em>Escherichia coli</em> after 120 min of K-GW treatment and were successfully reusable for five cycles with an inactivation efficiency >48 %. Pilot plant results with the PSG and MS deposited films, an overall efficiency for <em>E. coli</em> inactivation of 93 ± 6 and 88 ± 4 %, respectively, was obtained at 140 min, as well as an efficiency above 90 % for chemical oxygen demand and nitrite removal. In addition, the treated greywater favoured the “<em>in vitro</em>” germination of <em>Lactuca sativa</em> L. and <em>Lolium perenne</em> seeds and complied with 95 % of the U.S. standards for water reuse. The pilot plant with TiO<sub>2</sub> films offered an effective solution to inactivate <em>Escherichia coli</em> and remove contaminants.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108100"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linjin Li , Yaoze Wang , Guangfei Qu , Nanqi Ren , Yanping Liu , Jiao Wei , Dehui Kong , Changyi He , Minhua Cheng , Yingying Cai , Xiaofei Li , Rui Xu
{"title":"Mn-doped copper slag with oxygen vacancies boosts peroxymonosulfate activation for ofloxacin rapid removal","authors":"Linjin Li , Yaoze Wang , Guangfei Qu , Nanqi Ren , Yanping Liu , Jiao Wei , Dehui Kong , Changyi He , Minhua Cheng , Yingying Cai , Xiaofei Li , Rui Xu","doi":"10.1016/j.jwpe.2025.108151","DOIUrl":"10.1016/j.jwpe.2025.108151","url":null,"abstract":"<div><div>This study developed a manganese-doped copper smelting slag (Mn@CSS) particle electrode to enhance peroxymonosulfate (PMS) activation for efficient ofloxacin (OFX) degradation. The Mn(II) doping strategy effectively modulated surface oxygen vacancies, with the optimal 0.08 doping ratio achieving doubled oxygen vacancy concentration compared to pristine CSS. Density functional theory (DFT) calculations revealed significantly enhanced PMS adsorption capacity, demonstrating a 57 % increase in adsorption energy (ΔE<sub>ads</sub> = −3.31 eV for Mn@CSS vs. -2.10 eV for CSS). Notably, the O<img>O bond length extended from 1.45 Å to 1.74 Å upon adsorption, facilitating interfacial charge transfer. Under optimized conditions (pH 7.0, 1.50 g/L catalyst dosage, 20 mg/L initial OFX concentration, and 10 mA/cm<sup>2</sup> current density), the system achieved exceptional performance with 99.90 % OFX removal and 58.79 % total organic carbon reduction within 30 min. Mechanistic investigations through electron paramagnetic resonance and LC-MS analyses identified synergistic contributions from multiple reactive species (SO₄<sup>•-</sup>, O₂<sup>•-</sup>, <sup>•</sup>OH, <sup>1</sup>O₂, and Fe(IV)), driving mineralization via dealkylation, decarboxylation, and ring-opening pathways. The catalyst demonstrated remarkable stability with 87 % efficiency retention after five cycles and minimal metal leaching (Fe: 1.20 %, Mn: 0.79 %). This work not only provides a sustainable strategy for solid waste valorization but also elucidates the oxygen vacancy-mediated PMS activation mechanism through integrated experimental and theoretical approaches.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108151"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hongqi Jin , Zhicheng Wei , Qian Feng , Gang Zhao , Zhaoxia Xue , Yang Wu , Jingyang Luo
{"title":"Flow velocity-dependent methane emissions in sewage pipelines: Mechanistic insights from substrate metabolism to microbial trait dynamics","authors":"Hongqi Jin , Zhicheng Wei , Qian Feng , Gang Zhao , Zhaoxia Xue , Yang Wu , Jingyang Luo","doi":"10.1016/j.jwpe.2025.108174","DOIUrl":"10.1016/j.jwpe.2025.108174","url":null,"abstract":"<div><div>Urban sewer networks contribute significantly to greenhouse gas emissions through methane release, but the mechanisms controlling these emissions, particularly how flow velocity affects them, remain poorly understood. This study systematically investigated the impacts of flow velocity on methane emissions in sewage pipelines and revealed the underlying mechanisms through multiple analytical approaches. Results demonstrated that increasing flow velocity from 0.4 to 0.8 m/s significantly reduced cumulative methane production by 72.9 % within 72 h, with methane emission flux decreasing from 0.292 to 0.127 mg/(m<sup>2</sup>·h). Three-dimensional fluorescence spectroscopy analysis revealed that higher flow velocity limited substrate consumption, as evidenced by Region-IV fluorescence intensity being 48.1 % higher at 0.8 m/s than at 0.4 m/s. Microbial analysis showed that higher flow velocity significantly altered community structures, reducing key functional bacteria (<em>e.g.</em>, <em>Tolumonas</em> decreased from 2.85 to 2.14 %) and methanogens <em>(e.g.</em>, <em>Methanobacterium</em> decreased from 24.45 to 22.03 %), while diminishing community resilience and functional complementarity. Moreover, functional gene analysis further demonstrated that higher flow velocity downregulated genes involved in substrate hydrolysis (<em>e.g.</em>, <em>pgm</em>), volatile fatty acids synthesis (<em>e.g.</em>, <em>porA</em>), and methane production (<em>e.g.</em>, <em>mcrA</em>). Correlation analysis identified flow-induced changes in microbial community composition as the primary driver of methane production variability. These findings provide novel insights into flow velocity-dependent methane emission mechanisms in sewer systems.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108174"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lenka Urbánová , Marek Bujdoš , Martin Cesnek , Marcel Miglierini , Miroslav Čavojský , Peter Machata , Matej Mičušík , Jozef Kollár , Peter Matúš , Martin Urík
{"title":"Impact of temperature and humic acid-assisted synthesis on selenium sorption onto iron oxide nanoparticles","authors":"Lenka Urbánová , Marek Bujdoš , Martin Cesnek , Marcel Miglierini , Miroslav Čavojský , Peter Machata , Matej Mičušík , Jozef Kollár , Peter Matúš , Martin Urík","doi":"10.1016/j.jwpe.2025.108119","DOIUrl":"10.1016/j.jwpe.2025.108119","url":null,"abstract":"<div><div>Selenium is a key element for biological systems, but at elevated concentrations it can pose a risk to both the environment and human health. Therefore, developing effective strategies to limit its mobility is crucial. Here, we investigated the immobilization of selenite and selenate using iron-based magnetic adsorbents synthesized at various temperatures in the presence of humic acids (HA). Mössbauer spectrometry confirmed the presence of both (minor) magnetite and (major) maghemite components in the adsorbents. HA decreased the overall surface charge and size of the nanoparticles. Kinetic analysis revealed rapid and efficient removal of both species by the synthesized adsorbents at pH 3. The process was best described by pseudo-nth order kinetic model for selenite, whereas the kinetic data for selenate were inconclusive. This suggests a complex interaction between selenite and the adsorbent surfaces that cannot be adequately described by first- or second-order reaction kinetics since the sorption likely involved reductive immobilization, as evidenced by the presence of elemental selenium on the adsorbent surfaces, as revealed by X-ray photoelectron spectroscopy. Selenite sorption showed maximum capacity for adsorbents synthesized at 60 °C, without HA at 539.5 μmol⋅g<sup>−1</sup> and with HA at 528.4 μmol⋅g<sup>−1</sup>. The highest maximum sorption capacity of selenate was observed for materials synthesized without HA at 30 °C at 340.9 μmol⋅g<sup>−1</sup>. Desorption experiments demonstrated high regeneration efficiency for sorbents synthesized at lower temperatures, with desorption rates reaching up to 92 %. However, the presence of HA significantly reduced desorption efficiency, though this effect diminished as the synthesis temperature increased.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108119"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiang Zhang , Yingjie Song , Xiang Xiong , Zhaoyang Li , Weiming Yang , Haishun Liu
{"title":"A new insight into surface oxidation of Fe-based metallic glass for azo dye degradation","authors":"Xiang Zhang , Yingjie Song , Xiang Xiong , Zhaoyang Li , Weiming Yang , Haishun Liu","doi":"10.1016/j.jwpe.2025.108170","DOIUrl":"10.1016/j.jwpe.2025.108170","url":null,"abstract":"<div><div>Removing azo dyes from effluents poses an environmental and socio-economic challenge due to their carcinogenic and mutagenic risks. The Fenton-like reaction using Fe-based metallic glass (MG) is feasible for wastewater remediation; however, the oxidation-induced passive film will impede the further reaction of Fe-based catalysts, especially for Fe-based MG recycled from end-of-life soft magnetic products. Here, the oxidation behavior of Fe<sub>73.5</sub>Si<sub>13.5</sub>B<sub>9</sub>Cu<sub>1</sub>Nb<sub>3</sub> MG ribbons and the consequential effect on the degradation performance of acid orange 7 have been systematically investigated. When the Fe-based MG is oxidized at low temperatures (<520 °C), a continuous and dense oxide layer forms, shielding the zero-valence iron (ZVI) from interacting with dye molecules. As the oxidation temperature rises to 580–680 °C, numerous α-Fe(Si) nanocrystals and CuO nuclei will appear and degrade the protective oxide film during the rapid high-temperature oxidation (RHTO), enhancing the azo dye decolorization rate. Further oxidation at higher temperatures (>700 °C) will increase the oxide layer thickness and consume ZVI, thereby blocking the Fenton-like reactions. Notably, the RHTO could reactivate the inactivated Fe-based MG and endow the inactivated MG with a high dye degradation constant <em>k</em> of 1.5 min<sup>−1</sup>, indicating a promising method for reusing the end-of-life Fe-based MG electronic products for textile dye wastewater treatment.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108170"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haishuo Wang , Xinke Tian , Jie Zhang , Heyong Huang , Shiyin Li , Guoxiang Wang
{"title":"Photochemistry of Lake Taihu dissolved organic matter: Reactive species generation and effects on microcystin photodegradation","authors":"Haishuo Wang , Xinke Tian , Jie Zhang , Heyong Huang , Shiyin Li , Guoxiang Wang","doi":"10.1016/j.jwpe.2025.108161","DOIUrl":"10.1016/j.jwpe.2025.108161","url":null,"abstract":"<div><div>Dissolved organic matter (DOM) plays crucial roles in photochemical processes through light-induced transformations and reactive species generation. Four types – cyanobacteria extract from Lake Taihu (CDOM), reed leaf extract from Lake Taihu (RDOM), sodium humate (HA), and fulvic acid (FA) – were studied. The main component of all four DOM was humic acid-like, with fluorescence intensity reduced under light. Fourier-transform ion cyclotron resonance mass spectrometr (FT-ICR MS) revealed CDOM, RDOM, and FA contained abundant CHON and CHONS compounds, while HA was rich in CHO. Light exposure increased the number of molecular formulae in CDOM, RDOM, and FA. The unsaturation of CDOM, RDOM, and HA increased, while FA decreased. All DOM had high lignin abundance, with CDOM and RDOM also high protein and lipid. Lignin-like compounds decreased after 10 h light. DOM undergoes photochemical reactions under light to produce various reactive species, mainly excited triplet DOM (<sup>3</sup>DOM<sup>⁎</sup>), hydroxyl radicals (·OH) and singlet oxygen (<sup>1</sup>O<sub>2</sub>), CDOM produces the most ∙OH and <sup>3</sup>DOM<sup>⁎</sup>, and HA produces the most <sup>1</sup>O<sub>2</sub>. All four DOM types enhanced microcystin-LR (MCLR) photodegradation compared to no DOM, with FA showing the strongest promotion (29.2 % degradation). FA photolysis yielded higher ·OH and <sup>1</sup>O<sub>2</sub>, it was speculated that the conversion of <sup>3</sup>DOM<sup>⁎</sup> to ·OH and <sup>1</sup>O<sub>2</sub> may significantly impacts MCLR degradation. This study elucidates the differential effects of DOM from different sources on the photodegradation efficiency of MCLR due to their molecular composition and photochemical properties.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108161"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saeed Azizi , Songpei Xie , Anirban Ghosh , Timothy J. Hubin , Mark Krzmarzick , Clint P. Aichele
{"title":"Selective lanthanum recovery in the presence of sodium ions by N-(trimethoxysilypropyl)ethylenediamine triacetic acid (TMS-EDTriA)-modified silica","authors":"Saeed Azizi , Songpei Xie , Anirban Ghosh , Timothy J. Hubin , Mark Krzmarzick , Clint P. Aichele","doi":"10.1016/j.jwpe.2025.108106","DOIUrl":"10.1016/j.jwpe.2025.108106","url":null,"abstract":"<div><div>This study presents a novel approach investigating the silica particle modification using N-(trimethoxysilypropyl)ethylenediamine triacetic acid to enhance the recovery of lanthanum in three mixtures; 20 ppm La<sup>3+</sup>, 20 ppm La<sup>3+</sup> with 20 ppm Na<sup>+</sup>, and 20 ppm La<sup>3+</sup> with 100 ppm Na<sup>+</sup>. The influence of pH during modification was also tested. Characterization via Fourier Transform Infrared, Energy-dispersive X-ray Spectroscopy, and Thermogravimetric Analysis confirms the successful coating of propylene-(ethylenediamine)triacetic acid molecules on the silica surface, with modification under the basic conditions exhibiting the more accessible and stable coating. As a result, the La<sup>3+</sup> adsorption capacity of the modification under the basic conditions has been increased, making it suitable for effective recovery. Particles modified with a few drops of acid (0.075 mL L<sup>−1</sup>) recovered 95.27 % of La<sup>3+</sup> in 20 ppm La<sup>3+</sup> solution, while the unmodified particles recovered 82.62 %. The particles modified with 1.5 mL L<sup>−1</sup> of acid recovered only 11.18 %. In the mixed solutions, the performance of the basic-modified particles showed a selectivity of more than 98 %. The particles modified with 9 mL L<sup>−1</sup> of acid did not exhibit detectable La<sup>3+</sup> recovery in all types of solutions. Kinetic experiments show that modified particles with a few drops of acid (0.075 mL L<sup>−1</sup>) reach equilibrium for La sorption in less than 2 h, compared to 20 h for unmodified silica.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108106"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jinshan Ma, Ziqian Huang, Yuxin He, Chenxin Zhang, Guowei Chen, Li Liu
{"title":"Mechanisms for disinfection-driven conjugative spread of antibiotic resistance in drinking water biofilms","authors":"Jinshan Ma, Ziqian Huang, Yuxin He, Chenxin Zhang, Guowei Chen, Li Liu","doi":"10.1016/j.jwpe.2025.108175","DOIUrl":"10.1016/j.jwpe.2025.108175","url":null,"abstract":"<div><div>The persistent use of antibiotics has exacerbated the spread of antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in aquatic environments, including drinking water systems. Pipeline biofilms, comprising over 95 % of microbial biomass, function as high-risk reservoirs for ARG accumulation and horizontal gene transfer (HGT). However, the roles of disinfection-induced biofilm aggregation in plasmid-mediated conjugation remains mechanistically obscure. This study investigated how sodium hypochlorite (NaClO), chlorine dioxide (ClO<sub>2</sub>), and their combination affect ARB selection and ARG conjugation in multispecies drinking water biofilms at environmentally relevant chlorine concentrations (0–2.0 mg/L). Results showed that exposure to either NaClO or ClO<sub>2</sub> at 1.0 mg/L increased the relative abundance of ARB, while combined disinfection at 2.0 mg/L exerted selective pressure that further enhanced ARB enrichment. Sublethal chlorine levels increased conjugation frequencies within biofilms by 2.1- to 5.9-fold relative to untreated controls. Conjugation efficiency was positively correlated with bacterial surface colonization and metabolic activity (<em>r</em> = 0.95, <em>p</em> < 0.05). Mechanistic investigations revealed that chlorine exposure upregulated integrase genes, enriched potential ARG-hosting taxa, and preserved fimbrial structures, collectively facilitating donor-recipient interactions and promoting HGT. These findings elucidate the mechanistic pathways underlying disinfection-mediated horizontal gene transfer and provide critical insights into managing antibiotic resistance risks in drinking water systems.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108175"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shixin Jie , Jiangping Han , Shengwen Jia , Na Tan , Wenjie Jin
{"title":"Achromobacter sp. JWJ-09 based on methanol co-metabolism efficiently enhanced the degradation of PAHs in coking wastewater and domestic sewage using activated sludge","authors":"Shixin Jie , Jiangping Han , Shengwen Jia , Na Tan , Wenjie Jin","doi":"10.1016/j.jwpe.2025.108152","DOIUrl":"10.1016/j.jwpe.2025.108152","url":null,"abstract":"<div><div>Naphthalene, anthracene, and pyrene, representative polycyclic aromatic hydrocarbons (PAHs) with two, three, and four aromatic rings, respectively, possess genotoxic, carcinogenic, mutagenic, and teratogenic properties and are common refractory substances in domestic sewage and coking wastewater. Therefore, efficiently degrading PAHs in domestic sewage and coking wastewater is important. Using <em>Achromobacter</em> sp. JWJ-09 and 12 mg·L<sup>−1</sup> methanol as the degrading bacterium and co-metabolic substrate, respectively, the removal efficiencies of 100 mg·L<sup>−1</sup> naphthalene, 40 mg·L<sup>−1</sup> anthracene, and 60 mg·L<sup>−1</sup> pyrene reached 76.84 %, 65.77 %, and 72.87 %, respectively, in 120 h. Furthermore, GC–MS analysis showed that the degradation pathway of naphthalene is the protocatechuic acid pathway, whereas that of anthracene and pyrene is the phthalic acid metabolism pathway. To improve the PAHs removal efficiency in biological treatment processes, strain JWJ-09 and methanol were added to the activated sludge. The removal efficiencies of naphthalene, anthracene, pyrene, TN, and COD in domestic sewage increased by 20.81 %, 25.35 % and 37.3 %, 25.13 % and 11.26 %, compared to using activated sludge alone, whereas those in coking wastewater increased by 10.4 %, 11.74 %, 12.03 %, 15.5 % and 15.37 %, respectively. High-throughput sequencing revealed that JWJ-09 and methanol increased the abundance of phyla Actinobacteriota and Chloroflexi in domestic sewage activated sludge by 23.37 %, 10.06 %, and enriched bacterial genera. The abundances of Acidobacteriota, Chloroflexi and the genus <em>norank_ f_ _ Blastocatellacea</em> in activated sludge for the PAHs degradation in coking wastewater increased by 25.31 %, 3.49 % and 25.04 %, respectively. In conclusion, JWJ-09 and methanol co-metabolism enhanced activated sludge can treat domestic sewage and coking wastewater containing PAHs.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"76 ","pages":"Article 108152"},"PeriodicalIF":6.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}