Journal of Tropical Medicine最新文献

{"title":"Evaluation of <i>Chlamydia trachomatis</i> Genotypes in Endocervical Specimens by Sequence Analysis of ompA Gene among Women in Tehran.","authors":"Mohammadreza Rajabpour,&nbsp;Amir Darb Emamie,&nbsp;Mohammad Reza Pourmand","doi":"10.1155/2023/8845565","DOIUrl":"https://doi.org/10.1155/2023/8845565","url":null,"abstract":"<p><p>Tehran's actual prevalence of <i>Chlamydia trachomatis</i> (CT) and its genotypes are still unclear. Molecular typing of CT strains can provide essential epidemiological knowledge and contribute to improved control measures. In this study, we aimed to determine the prevalence of CT and its genotypes in the endocervical infections of females who attended the gynecology and infertility clinics in Tehran. A total of 291 women were tested for chlamydial infection by in-house PCR using specific primers for the CT cryptic plasmid. Nested PCR for amplification of the ompA gene in positive samples was carried out, genotyping was performed by sequencing this gene, and further phylogenetic analysis was conducted. Sexual infection by CT was observed in 10.3% (30/291) of the subjects, and the mean age of patients was 30.4. The ompA gene was sequenced in 27 samples, revealing E genotypes 40.7%, (<i>n</i> = 11), F 25.9%, (<i>n</i> = 7), G 18.5%, (<i>n</i> = 5), D 11.1%, (<i>n</i> = 3), and K 3.7%, (<i>n</i> = 1). This study emphasizes the importance of the diversity among CT genotypes in our studied population and the need for wide-screening the neglected bacterial infection among women in Tehran.</p>","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2023 ","pages":"8845565"},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9956779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Genotype of <i>Trichomonas vaginalis</i> among Men in Xinxiang City, Henan Province, China.","authors":"Zhenchao Zhang,&nbsp;Yuhui Sang,&nbsp;Pucheng Wu,&nbsp;Yujia Shang,&nbsp;Lesong Li,&nbsp;Yujuan Duan,&nbsp;Linfei Zhao,&nbsp;Minghui Gao,&nbsp;Lihua Guo,&nbsp;Xiaowei Tian,&nbsp;Zhenke Yang,&nbsp;Shuai Wang,&nbsp;Lixia Hao,&nbsp;Xuefang Mei","doi":"10.1155/2023/4119956","DOIUrl":"https://doi.org/10.1155/2023/4119956","url":null,"abstract":"<p><p><i>Trichomonas vaginalis</i> (<i>T. vaginalis</i>) could cause trichomoniasis through sexual transmission, which was globally distributed. In this study, the prevalence and phylogenetic analyses of <i>T. vaginalis</i> among men in Xinxiang were conducted. From October 2018 to December 2019, a total of 634 male clinical samples were collected, including 254 samples of semen, 43 samples of prostate fluid, and 337 samples of urine. These samples were examined by nested PCR and a total of 32 (5.05%) <i>T. vaginalis</i>-positive samples were detected. Among these samples, the positive rates of <i>T. vaginalis</i> in semen, prostate fluid, and urine were 7.87% (20/254), 4.65% (2/43), and 2.97% (10/337), respectively. Three actin genes were successfully isolated and sequenced from the 32 positive DNA samples, and the analysis of the sequence and phylogenetic tree showed that the three actin gene sequences exhibited 99.7%-100% homology to the published actin gene sequence (EU076580) in NCBI, and the <i>T. vaginalis</i> strains in the three positive samples were identified as genotype E. Our results demonstrate a notable genotype of <i>T. vaginalis</i> in the male population and provide insight into the performance of these genetic markers in the molecular epidemiology of trichomoniasis. However, further studies are needed to research the association between the genotype and the pathogenicity of <i>T. vaginalis</i>.</p>","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2023 ","pages":"4119956"},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9991476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9087517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution, Prevalence, and Antibiotic Susceptibility Profiles of Infectious Noncholera <i>Vibrio</i> Species in Malaysia.","authors":"Murnihayati Hassan,&nbsp;Mohammad Ridhuan Mohd Ali,&nbsp;Hana Farizah Zamri,&nbsp;Nur Asyura Nor Amdan,&nbsp;Mohammad Noor Amal Azmai,&nbsp;Saraswathiy Maniam,&nbsp;Norfarrah Mohamed Alipiah,&nbsp;Rohaidah Hashim","doi":"10.1155/2023/2716789","DOIUrl":"https://doi.org/10.1155/2023/2716789","url":null,"abstract":"<p><strong>Background: </strong>The noncholera <i>Vibrio</i> spp. which cause vibriosis are abundantly found in our water ecosystem. These bacteria could negatively affect both humans and animals. To date, there is a paucity of information available on the existence and pathogenicity of this particular noncholera <i>Vibrio</i> spp. in Malaysia in comparison to their counterpart, <i>Vibrio cholera</i>.</p><p><strong>Methods: </strong>In this study, we extracted retrospective data from Malaysian surveillance database. Analysis was carried out using WHONET software focusing noncholera <i>Vibrio</i> spp. including <i>Vibrio parahaemolyticus</i>, <i>Vibrio vulnificus</i>, <i>Vibrio fluvialis</i>, <i>Vibrio alginolyticus</i>, <i>Vibrio hollisae</i> (<i>Grimontia hollisae</i>), <i>Vibrio mimicus</i>, <i>Vibrio metschnikovii</i>, and <i>Vibrio furnissii</i>.</p><p><strong>Results: </strong>Here, we report the first distribution and prevalence of these species isolated in Malaysia together with the antibiotic sensitivity profile based on the species. We found that <i>V. parahaemolyticus</i> is the predominant species isolated in Malaysia. Noticeably, across the study period, <i>V. fluvialis</i> is becoming more prevalent, as compared to <i>V. parahaemolyticus</i>. In addition, this study also reports the first isolation of pathogenic <i>V. furnissii</i> from stool in Malaysia.</p><p><strong>Conclusion: </strong>These data represent an important step toward understanding the potential emergence of noncholera <i>Vibrio</i> spp. outbreaks.</p>","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2023 ","pages":"2716789"},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10238136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9578374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Asymptomatic Malaria Parasitemia among Blood Donors in Cape Coast, Ghana: A Cross-Sectional Study.","authors":"Ato Kwamena Tetteh,&nbsp;Sadick Arthur,&nbsp;Prince Bram,&nbsp;Charles Baffe,&nbsp;Godsway Aglagoh","doi":"10.1155/2023/8685482","DOIUrl":"https://doi.org/10.1155/2023/8685482","url":null,"abstract":"<p><strong>Background: </strong>Malaria is an important transfusion-associated infection in many parts of the world, particularly in sub-Saharan Africa, where it is endemic. We studied the prevalence of malaria parasites among blood donors in the Cape Coast Metropolitan Area.</p><p><strong>Methods: </strong>A malaria parasite examination was added to the blood donor screening protocol for 240 voluntary and replacement blood donors (224 males and 16 females) between December 2020 and July 2021.</p><p><strong>Results: </strong>Overall, 2.5% (6/240) had <i>Plasmodium falciparum</i> trophozoites detected in their blood sample. The remaining had no parasites detected. Four of the 148 who passed the blood donor screening tests were infected. The remaining two with malaria parasites failed one screening test. These included one donor with \"hepatitis <i>B</i> + <i>P. falciparum</i>\" and another with \"syphilis + <i>P. falciparum</i>\" parasite coinfection. All blood donors who had malaria parasites detected in their blood were males. Most donors, 45.8% (110/240), were in the 26-35 age group, with the highest prevalence of 1.3% (3/240). Blood group O was predominant (75.0%, 180/240), followed by B (12.9%, 31/240), A (11.3%, 27/240), and AB (0.8%, 2/240). All malaria parasites detected were among individuals with blood group O. Moreover, 96.3% (231/240) were rhesus-positive and had the highest prevalence of 2.1% (5/240).</p><p><strong>Conclusions: </strong>Screening of blood donors in Ghana does not include malaria, although there is the potential for transmission through blood products. Malaria transmission via blood transfusion remains an issue of public health concern, as indicated in the results of this current study. We recommend studies on malaria prevention, pretransfusion and posttransfusion, and pathogen reduction technology.</p>","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2023 ","pages":"8685482"},"PeriodicalIF":2.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9833917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10590111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Mass Drug Administration on Lymphatic Filariasis","authors":"I. F. Aboagye, Yvonne Abena Afadua Addison","doi":"10.1155/2022/7504871","DOIUrl":"https://doi.org/10.1155/2022/7504871","url":null,"abstract":"Mass drug administration (MDA) has made a significant impact on the control of lymphatic filariasis (LF) since the establishment of the Global Programme to Eliminate Lymphatic Filariasis. However, its implementation is associated with several challenges, hampering interruption of parasite transmission and LF elimination in endemic areas. This study assessed the impact of MDA by comparing baseline microfilaria and antigen prevalence with those after three years (mid-term) and ≥5 years of MDA implementation and their respective prevalence reductions and identified specific challenges that may hinder its effective implementation. Three years of MDA implementation were observed to have microfilaria prevalence reductions (88.54% to 98.66%) comparable to those of studies that implemented MDA for five to 10 years (≥5 years, 79.23% to 98.26%). Inadequate community understanding of and participation in the LF MDA programme are major drawbacks to its effective implementation. The implementation of MDA that incorporates community participation, incentivisation, education, and training strategies has the potential of increasing MDA coverage and compliance, thereby interrupting parasite transmission and reducing microfilarial prevalence to levels that warrant LF elimination.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42822802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demographic and Clinical Determinants of Tuberculosis and TB Recurrence: A Double-Edged Retrospective Study from Pakistan.","authors":"Mariam Ahmed Mujtaba, Matthew Richardson, Hira Shahzad, Muhammad Ishaq Javed, Ghazala Kaukab Raja, Pakeeza Arzoo Shaiq, Pranabashis Haldar, Sadia Saeed","doi":"10.1155/2022/4408306","DOIUrl":"10.1155/2022/4408306","url":null,"abstract":"<p><strong>Objective: </strong>TB recurrence is the second episode of TB after initial treatment bringing about an additional 7% load in TB burden intensified by 17.7% of multidrug-resistant recurrent cases. It is necessary to curb recurrence so that attempts to deal with active disease can be made more effective. This study aimed to characterize sociodemographic and clinical factors associated with recurrent TB in a high-burden setting. <i>Methodology</i>. A retrospective case-control study was carried out at two hospitals in Rawalpindi, Pakistan. TB patients and controls were included in the study. Sociodemographic and clinical data were collected by questionnaire from all subjects. Multivariate regression analysis was performed to determine factors associated with TB and TB recurrence respectively.</p><p><strong>Results: </strong>In our study cohort, factors significantly associated with TB were low BMI (OR: 0.961 (CI 0.954-0.968), <i>p</i> < 0.001), female gender (OR: 2.065 (CI 1.922-2.219), <i>p</i> < 0.001), being single/unmarried (OR: 1.214 (CI 1.109-1.328), <i>p</i>=0.003), middle-income status (OR: 1.935 (CI 1.616-2.323), <i>p</i> < 0.001), smoking (OR: 1.567 (CI 1.435-1.710), <i>p</i> < 0.001), and diabetes mellitus (OR: 1.142 (CI 1.017-1.278), <i>p</i>=0.023). TB recurrence constituted 11.2% of patients presenting to the hospital. Compared with the first episode of TB, cases with recurrence were more likely to be older (OR: 1.011 (CI 1.004-1.017), <i>p</i> < 0.001), have disease awareness (OR: 1.906 (CI 1.486-2.437), <i>p</i> < 0.001), smear positive (OR: 2.384 (CI 1.650-3.536), <i>p</i> < 0.001), and be drug-resistant (OR: 5.615 (CI 4.265-7.386), <i>p</i> < 0.001).</p><p><strong>Conclusion: </strong>In the present study cohort, low BMI, female gender, being single, middle-income status, being unemployed, smoking, and being diabetic came out to be the sociodemographic and clinical risk factors for TB. Further exploring the TB cases increasing age, drug resistance and smear positivity stood out to be the major sociodemographic and clinical factors of TB recurrence despite high disease awareness.</p>","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2022 ","pages":"4408306"},"PeriodicalIF":2.1,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10722027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate Change and Environmental Influence on Prevalence of Visceral Leishmaniasis in West Pokot County, Kenya.","authors":"Bulle Abdullahi, Joshua Mutiso, Fredrick Maloba, John Macharia, Mark Riongoita, Michael Gicheru","doi":"10.1155/2022/1441576","DOIUrl":"10.1155/2022/1441576","url":null,"abstract":"<p><p>Kala-azar is a parasitic disease caused by <i>Leishmania species</i> transmitted by sand fly. In Kenya, kala-azar is endemic in thirty subcounties spread over in eleven counties in the arid zones. Climate change-influenced seasonal weather variability and environmental alterations remain important determinants of many vector-borne diseases. The present study focused on climate change and environmental influence on kala-azar in West Pokot. A descriptive cross-sectional and retrospective research design was adapted. Study area was purposively selected. Locations were randomly selected, and households were systematically selected. Three hundred sixty-three household questionnaires, eleven key informant interviews, and five focus group discussions were undertaken. Secondary data were obtained from Kacheliba subcounty hospital records. Statistical Package for the Social Sciences version 24 was used to analyze quantitative data while qualitative data were analyzed to establish connection for interpretation. Kala-azar cases have been on the rise on aggregate and surge towards the end of dry season and just after the rains. Significant environmental factors included the presence of seasonal rain water pathways and rock piles around houses (AOR = 4.7; 95% CI = (2.3-9.6), <i>p</i> < 0.05), presence of acacia trees in and around homesteads (AOR = 8.5; 95% CI = (2.5-28.6), <i>p</i> < 0.05), presence of anthills around the homesteads (AOR = 5.2; 95% CI = (1.2-23.4), <i>p</i> < 0.05), and presence of animal shed within compound (AOR = 2.8; 95% CI = (0.96-8), <i>p</i> < 0.05). Climate change-induced seasonal weather variability, increased temperature and reduced precipitation as well as environmental alterations influence kala-azar occurrence in West Pokot. Community sensitization on disease prevalence, clearing of vector predilection sites, and improving community environmental risk perception are imperative to promote prevention.</p>","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"1 1","pages":"1441576"},"PeriodicalIF":2.1,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11390187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41414941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic Analysis of Key Regulatory Metabolites in the Urine of Flavivirus-Infected Mice","authors":"Xiaoyan Zheng, Ran Wang","doi":"10.1155/2022/4663735","DOIUrl":"https://doi.org/10.1155/2022/4663735","url":null,"abstract":"Objective Dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV) are several important flaviviruses, and infections caused by these flaviviruses remain worldwide health problems. Different flaviviruses exhibit different biological characteristics and pathogenicity. Metabolomics is an emerging research perspective to uncover and observe the pathogenesis of certain infections. Methods To improve the understanding of the specific metabolic changes that occur during infection with different flaviviruses, considering the principle of noninvasive sampling, this article describes our comprehensive analysis of metabolites in urine samples from the three kinds of flavivirus-infected mice using a liquid chromatography tandem mass spectrometry method to better understand their infection mechanisms. Results The urine of DENV-, JEV-, and ZIKV-infected mice had 68, 64, and 47 different differential metabolites, respectively, compared with the urine of control mice. Among the metabolic pathways designed by these metabolites, ABC transporters, arginine and proline metabolism, and regulation of lipolysis play an important role. Furthermore, we predicted and fitted potential relationships between metabolites and pathways. Conclusions These virus-specific altered metabolites may be associated with their unique biological properties and pathogenicity. The metabolomic analysis of urine is very important for the analysis of flavivirus infection.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44947967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leishmania (Leishmania) mexicana Infection in Wild Rodents from an Emergent Focus of Cutaneous Leishmaniasis in Yucatan, Mexico","authors":"E. I. Sosa-Bibiano, Luis A Sánchez-Martínez, K. López-Ávila, J. Chablé-Santos, J. R. Torres-Castro, E. Fernández-Figueroa, C. Rangel-Escareño, E. N. Loría-Cervera","doi":"10.1155/2022/8392005","DOIUrl":"https://doi.org/10.1155/2022/8392005","url":null,"abstract":"In 2015, emergent cases of localized cutaneous leishmaniasis (LCL) were reported in Tinum, Yucatan, Mexico. As part of an eco-epidemiological study to characterize the elements that trigger Leishmania infection in that area, we conducted a field study to investigate the occurrence of Leishmania infection in wild rodents. From November 2019 to February 2020, rodents were caught from three sites located in the municipality of Tinum, Yucatan. For each specimen, clinical signs suggestive of Leishmania infection were recorded. Samples from the tail, liver, and spleen were taken for the identification of Leishmania DNA by PCR. Twenty rodents belonging to two species were caught including Heteromys gaumeri (55%, 11/20) and Ototylomys phyllotis (45%, 9/20). Fifty-five percent of the animals presented white spots on the tail, 15% had splenomegaly, and 5% had hepatomegaly. Fifty-five percent (11/20) of the animals were found infected by Leishmania. Heteromys gaumeri was caught in all trapping sites and was the most infected species (63.6%, 7/11). The percentage of infection for O. phyllotis was 44.4% (4/9). Leishmania (Leishmania) mexicana was identified as the infecting species in two H. gaumeri. This study provides, for the first time, evidence of Leishmania infection in wild rodents from the Yucatan state. Heteromys gaumeri and O. phyllotis may be involved in the transmission cycle of L. mexicana in this emergent focus; however, further longitudinal studies are needed to confirm their role as primary reservoirs.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45647621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview on Cryptosporidium bovis and Its Effect on Calves in Some Governorates in Egypt","authors":"A. Abdelaziz, Amin Tahoun, H. El-Sharkawy, Moustafa M. Abd El-Salam, Mohammed Alorabi, A. El-Shehawi, Rasha A. El Meghanawy, E. Toukhy, A. A. Abd El-Salam, S. Sorour","doi":"10.1155/2022/4271063","DOIUrl":"https://doi.org/10.1155/2022/4271063","url":null,"abstract":"The present study was conducted to elucidate the prevalence of Cryptosporidium bovis in suckling and weaned cattle calves (Bubalus bubalis) from different governorates in northern, middle, and southern Egypt, such as Behera, Menofia, Qaliubiya, Assiut, and Sohag; result revealed that from the overall examined fecal samples (n = 825), the overall prevalence was 7.27%, the highest significant infection rate was in young suckling calves less than one month (8.2%), and seasonally, winter season has the highest significant level (11.24%), but sex and locality were of no significant effect on the prevalence of infection in this study. Gene sequencing and phylogenetic analysis of the 18SSU-rRNA gene of the local bovine isolate were performed, and it was found that C. bovis genotype was highly similar to human isolate, which provoke the zoonotic transmission of bovine isolate to humans and identified as a potential source for human cryptosporidiosis infection in Egypt.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45568079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}