The Frequency of Intestinal Parasitic Infections in COVID-19 Patients: A Case-Control Study in Tehran, Capital of Iran.

IF 2.1 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Journal of Tropical Medicine Pub Date : 2023-09-08 eCollection Date: 2023-01-01 DOI:10.1155/2023/5359823
Ali Taghipour, Majid Pirestani, Ramin Hamidi Farahani, Mohammad Barati
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引用次数: 0

Abstract

The present study was done to evaluate the prevalence of intestinal parasitic infections (IPIs) in patients with COVID-19 in health care centers (Imam Reza and Golestan hospitals), Tehran, capital of Iran. By designing a matched case-control study, 200 fecal samples were collected for each of the COVID-19 patients and healthy individuals. Nasopharyngeal/oropharyngeal swab samples were collected from all participants for the diagnosis of COVID-19. RNA extraction was performed, and then real time reverse-transcription polymerase chain reaction (rRT-PCR) assay was applied to detect viral RNA. Considering the lung complications, 25%> lung complications was detected in 49 patients, 25-49% in 42 patients, and 50%≤ in 109 patients. Fecal samples were examined using different parasitological techniques. After nested-PCR, sequencing was applied to identify Cryptosporidium spp. and microsporidia spp. A relatively lower prevalence of IPIs was detected among control group (7.5%), than in COVID-19 patients (13%), though not significant (P=0.13). The most prevalent parasite among patients was Blastocystis sp. (6%). Also, 13.76% of IPIs were detected in inpatients with more than 50% lung complication. As well, a remarkably significant difference in IPIs was observed among diarrheic COVID-19 patients, in comparison with nondiarrheic patients (P < 0.00001). Moreover, the isolated sequences in the present study belonged to C. parvum subtype IIa and Enterocytozoon bieneusi genotypes D and Peru 8. In conclusion, more epidemiological and clinical research studies are needed to better understand the status and interaction of IPI in COVID-19 in Iran and other countries.

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新冠肺炎患者肠道寄生虫感染的频率:伊朗首都德黑兰的病例对照研究。
本研究旨在评估伊朗首都德黑兰医疗中心(Imam Reza和Golestan医院)新冠肺炎患者肠道寄生虫感染(IPI)的患病率。通过设计一项匹配的病例对照研究,为每个新冠肺炎患者和健康人收集了200份粪便样本。采集所有参与者的鼻咽/口咽拭子样本,用于诊断新冠肺炎。进行RNA提取,然后应用实时逆转录聚合酶链式反应(rRT PCR)检测病毒RNA。考虑到肺部并发症,49例患者的肺部并发症检出率为25%>,42例患者为25%-49%,109例患者为50%≤。使用不同的寄生虫学技术对粪便样本进行检查。nested-PCR后,应用测序来鉴定隐孢子虫属和微孢子虫属。对照组(7.5%)的IPI患病率相对低于新冠肺炎患者(13%),但并不显著(P=0.13)。患者中最流行的寄生虫是芽囊孢子虫属(6%)。此外,13.76%的IPIs是在肺部并发症超过50%的住院患者中检测到的。此外,与非腹泻患者相比,新冠肺炎腹泻患者的IPI存在显著差异(P<0.00001)。此外,本研究中分离的序列属于微小C.parvum亚型IIa和肠细胞体双neusi基因型D和Peru 8。总之,需要进行更多的流行病学和临床研究,以更好地了解伊朗和其他国家新冠肺炎IPI的状况和相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Tropical Medicine
Journal of Tropical Medicine Immunology and Microbiology-Parasitology
CiteScore
3.90
自引率
4.50%
发文量
0
审稿时长
14 weeks
期刊介绍: Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.
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