Journal of Tissue Engineering最新文献

{"title":"Synergistic Wnt/BMP Co-activation accelerates osteogenic differentiation of human pluripotent stem cells via paraxial mesoderm induction.","authors":"Hongsik Kim, Sujin Kim, A-Hyeon Kim, Hamyoung Lee, Seongwoo Jeong, Hayoun Kim, Jungbum Kim, Wonhwa Lee, Hwan Drew Kim","doi":"10.1177/20417314261468879","DOIUrl":"10.1177/20417314261468879","url":null,"abstract":"<p><p>Bone healing is a complex and well-organized process, regulated by various factors ranging from growth factors to hormones, cytokines, mechanical stimuli, and aging. Recently, numerous techniques have been devised to efficiently induce the differentiation of human induced pluripotent stem cells (hiPSCs) to osteoblasts. However, enhancing the efficiency of osteoblast differentiation remains a challenge. Thus, we induced the differentiation of hiPSCs to mesodermal cells through Wnt/BMP signaling based on the generation of hiPSCs. After successful generation of hiPSCs, we induced the differentiation of mesodermal cells to osteoblasts. The results revealed that the runt-related transcription factor 2 (<i>RUNX2</i>)-encoding gene was upregulated from the early differentiation stage; hence, the expression of the mature osteoblast marker was higher compared to that observed in other differentiation stages. In addition, the deposition of substrates in mature bones was observed. The results were confirmed via real-time PCR, Alizarin Red Staining, and Von Kossa staining. The coactivation of Wnt and BMP signaling was shown to rapidly and effectively promote the differentiation of osteoblasts. The findings of this study will provide a foundation for future studies on the mechanism of osteoblast development, as well as the biological and pathological investigations of drug screening and bone regeneration tracing.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261468879"},"PeriodicalIF":10.1,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13369415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148456201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-engineered blood vessels for clinical translation: Design logic, representative advances and persistent barriers.","authors":"Junjie Chen, Jiayang He, Dujiang Yang, Yuhan He, Junhao Zhang, Chunshui He, Wei Zeng","doi":"10.1177/20417314261466954","DOIUrl":"10.1177/20417314261466954","url":null,"abstract":"<p><p>Tissue-engineered blood vessels are promising alternatives for small-diameter vascular reconstruction, but clinical translation remains limited by thrombosis, compliance mismatch, delayed endothelialization and unstable host remodeling. This review synthesizes representative advances in scaffold design, seed-cell selection, bioactive regulation and biofabrication, emphasizing how these components interact under hemodynamic load. Rather than cataloguing technologies, we argue that translational success depends on matching material architecture, cellular phenotype, immune remodeling and manufacturing constraints to specific clinical indications. Acellular or host-repopulating grafts may be most realistic for urgent trauma or vascular access, whereas coronary and distal peripheral reconstruction require tighter control of endothelialization and mechanics. Future tissue-engineered vessels should be evaluated as indication-specific products with clinically meaningful benchmarks beyond short-term patency.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261466954"},"PeriodicalIF":10.1,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13342367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism-guided biomaterial strategies for intervertebral disc degeneration: Pathological heterogeneity, functional classification, and translational perspectives.","authors":"Jianpeng Chen, Wenhao Deng, Shiran Zhou, Jun Yan, Kai Chen, Qianliang Wang, Shujun Lv","doi":"10.1177/20417314261464764","DOIUrl":"10.1177/20417314261464764","url":null,"abstract":"<p><p>Intervertebral disc degeneration (IVDD) is a multifactorial and clinically heterogeneous condition involving disturbances in oxidative balance, mitochondrial homeostasis, inflammatory signaling, susceptibility to regulated cell death, extracellular matrix integrity, and biomechanical function. Although biomaterial-based strategies have shown significant promise in preclinical studies, their clinical translation remains hindered by pathological heterogeneity, insufficient mechanistic validation, and frequent reliance on simplified experimental models. These models often fail to fully replicate the chronic, mechanically complex, and clinically diverse nature of human disc degeneration. In addition, current biomaterial strategies are commonly classified according to material composition or isolated molecular targets, potentially obscuring their functional objectives and limiting mechanistic comparisons across therapeutic platforms. This review presents a mechanism-guided framework for interpreting biomaterial interventions in IVDD. Biomaterial strategies are discussed based on their primary pathological targets and therapeutic intentions, including inflammatory regulation, restoration of redox homeostasis, mitochondrial protection, ferroptosis modulation, extracellular matrix preservation, and multifunctional microenvironment-responsive interventions. This framework acknowledges that these pathological processes are interconnected and vary in their relative dominance across clinical and pathological contexts and patient populations. We further analyze current biomaterial strategies according to mechanistic intervention layers, including upstream sensing and initiation-level control, restraint of intracellular amplification, organelle stabilization, regulation of execution checkpoints, and integration with higher-order structural organization. This layered perspective emphasizes that the efficacy of biomaterials depends not only on their composition but also on the pathological context, depth of regulatory influence, mechanistic specificity, and compatibility across intervention layers. Collectively, this review provides an integrated, mechanism-oriented framework for biomaterial design, preclinical evaluation, and translational development in IVDD therapy.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261464764"},"PeriodicalIF":10.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13323677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering next-generation organoids: A review on bioprinting strategies, bioink innovations, and frontier applications.","authors":"Defeng Sun, Xuemei Fan, Hua Sun, Dongmei Li, Ruijia Shen, Pu Xia, Zhenying Zhao","doi":"10.1177/20417314261459941","DOIUrl":"10.1177/20417314261459941","url":null,"abstract":"<p><p>While organoids hold immense promise as in vitro three-dimensional (3D) models, their translational utility is fundamentally constrained by passive diffusion limits (>800 μm), which inevitably trigger necrotic core formation and stochastic structural heterogeneity. This review elucidates how bioprinting shatters these physical bottlenecks by executing a paradigm shift toward spatiotemporal determinism. We systematically decode the mechanobiological evolution of bioinks-charting the transition from exogenous static matrices, which now function as temporal controllers via tunable stress relaxation to direct YAP/TAZ mechanotransduction, to the emerging paradigm of \"engineerable living bioinks\" driven by endogenous, cadherin-mediated fluid-to-solid jamming transitions. Furthermore, we critically evaluate frontier spatial strategies, highlighting how sacrificial networks and deterministic multi-material assembly establish active convective infrastructures and precise biophysical boundary conditions. By enforcing this rigorous baseline, these technologies definitively rectify pharmacokinetic/pharmacodynamic (PK/PD) distortions-eradicating false-positive noise in high-throughput screening and bridging the post-implantation mass transport vacuum-elevating organoids from stochastic clusters to highly predictive pathophysiological macro-models. Ultimately, we posit that transitioning from isolated morphological fabrication to resolving the inherent systemic metabolic paradoxes of multi-lineage integration is the absolute prerequisite for clinical translation.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261459941"},"PeriodicalIF":10.1,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13263509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical regulation of mesenchymal stem cell osteogenesis, bone matrix homoeostasis, and skeletal pathology.","authors":"Udipt R Das, Hussain Jaffery, Penelope M Tsimbouri, Matthew J Dalby","doi":"10.1177/20417314261454194","DOIUrl":"10.1177/20417314261454194","url":null,"abstract":"<p><p>Osteogenesis is the process by which mesenchymal stem/stromal cells (MSCs) differentiate into mature osteoblasts, forming a mineralised bone matrix. This process is regulated by soluble factors, mechanical stimuli, and the extracellular matrix (ECM), which together maintain bone and mineral homoeostasis. Mechanotransduction, the conversion of physical cues into intracellular signals, is crucial for MSC fate determination and orchestrates bone matrix remodelling, balancing formation and resorption. Continuous mechanical loading supports optimal osteogenic differentiation, whereas mechanical unloading (sub-physiological mechanical loading) disrupts this equilibrium and increases bone loss risk. These processes involve complex crosstalk among local and systemic factors, immune cells, and osteoblast-osteoclast interactions in response to mechanical cues. This review discusses key biomechanical factors regulating MSC osteogenic differentiation and bone remodelling, and synthesises evidence on skeletal immobilisation and other unloading-associated conditions that contribute to skeletal anomalies. It further emphasises nanovibrational stimulation as a novel approach to enhance MSC osteogenesis and mitigate skeletal anomalies.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261454194"},"PeriodicalIF":10.1,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13237463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondrocyte metabolic transition from proliferation to quiescence revealed by FLIM in postnatal mouse knee joints.","authors":"Nadezda Ignatyeva, Boris Yakimov, Anastasiia D Kurenkova, Irina A Romanova, Pavel D Kibirskiy, Nikita Gavrilov, Anastasiya Patsyurkevich, Irina D Shcherbakova, Artem M Mozherov, Aleksandra V Kashina, Evgeny A Shirshin, Peter S Timashev, Ekaterina V Medvedeva","doi":"10.1177/20417314261432891","DOIUrl":"10.1177/20417314261432891","url":null,"abstract":"<p><p>Adult articular cartilage chondrocytes have a limited capacity to divide compared to juvenile cells, but the mechanisms behind this decline remain unclear. This study investigates metabolic changes associated with the cessation of chondrocyte proliferation in mouse articular cartilage. Using 5-ethynyl-2'-deoxyuridine (EdU) labeling, the postnatal decline in proliferation was tracked. Label-free fluorescence-lifetime imaging microscopy (FLIM) method, combined with artificial intelligence (AI)-assisted image segmentation, was applied to live cartilage sections to analyze metabolic parameters. Results showed that 1-month-old articular cartilage chondrocytes enter quiescence with significant changes in FLIM fluorescence decay parameters across cartilage zones compared to juvenile chondrocytes. Chondroprogenitors in the superficial zone showed a gradual decrease in citrate synthase content, while glycolytic activity increased with tissue depth. These findings reveal metabolic reprogramming that enables chondrocytes to adapt their metabolism despite limited oxygen availability to meet functional demands. Investigating these chondrocyte adaptations provides key insights for identifying metabolic targets and improving the design of durable, well-integrated tissue-engineered cartilage.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261432891"},"PeriodicalIF":10.1,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13198642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-free therapy for alopecia via the secretome of hiPSC-derived dermal papilla cells.","authors":"Hyesoo Hwangbo, Aoyang Pu, Wanyu Tan, Eunice Dotse, Huanhuan Sun, Xin Gan, Yun-Gwi Park, Yimin Lai, Soon-Jung Park, Inho Choi, In-Rok Oh, Kwan Ting Chow, Sung-Hwan Moon, Hae-Won Kim, Byung Cheol Park, Kiwon Ban","doi":"10.1177/20417314261449597","DOIUrl":"10.1177/20417314261449597","url":null,"abstract":"<p><p>Alopecia is highly prevalent and debilitating, yet current drugs provide limited, reversible benefit with notable side effects. We established a rapid protocol to generate human induced pluripotent stem cell-derived dermal papilla cells (hiPSC-DPCs) and demonstrated that their conditioned medium (CM) acts as a potent, cell-free hair-regenerative therapy. Transdermal delivery of hiPSC-DPCs or CM accelerated anagen re-entry and hair regrowth in depilated mice, and hiPSC-DPC CM outperformed minoxidil in promoting ex vivo hair-shaft elongation and in vitro proliferation and migration of primary DPCs and keratinocytes. Proteomic and metabolomic profiling revealed enrichment of growth factors, antioxidants, and immunomodulatory metabolites linked to TNF and PI3K-Akt signaling, conferring superior anti-inflammatory and cytoprotective properties relative to primary DPC CM. Moreover, hiPSC-DPC CM mitigated dihydrotestosterone (DHT)-induced pathology by suppressing androgen receptor expression and nuclear translocation. These findings position hiPSC-DPC secretome as a dual-functional, regenerative, and anti-androgenic biologic with translational potential for durable alopecia treatment.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261449597"},"PeriodicalIF":10.1,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13191134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold-stimulated browning graft fat enhances burn wound recovery through accelerating DAMP-ADSC activation.","authors":"Wenhui Ma, Zhenyu Quan, Li Jiang, Pengya An, Qi Zhao, Yucheng Luo, Yueqi Zhang, Xiaohua Feng, Yong Pan","doi":"10.1177/20417314261451461","DOIUrl":"10.1177/20417314261451461","url":null,"abstract":"<p><p>Suboptimal skin regeneration in patients with severe burns leads to significant trauma. Due to their favorable biological properties, fat grafts have been widely used in wound repair. The present study aimed to investigate the regenerative benefits of cold-stimulated fat graft in a contact burn model using C57BL/6J mice. Transplantation of browning fat grafts, with enhanced adipogenic capacity and decreased fibrosis, effectively promoted granulation tissue thickness and re-epithelialization areas. Cold-stimulated fat graft had a significant accumulation of ADSCs, which migrated into wound skin. In adipocyte-deficient mice, the impaired wound-healing phenotype was reversed by browning fat graft. Cold-stimulated fat also exhibited higher levels of damage-associated molecular patterns (DAMPs), which induced the proliferation of ADSCs and promoted ADSC proliferation and differentiation into mature adipocytes. Inhibition of DAMP-related signaling abolished the repair benefits of browning fat graft. In conclusion, transplantation of cold-stimulated fat represents a highly effective strategy for treating burn wounds through promoting DAMP signaling and dermal adipose remodeling.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261451461"},"PeriodicalIF":10.1,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13187421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically modified bone marrow cells halt mitral cell loss by modulating inflammation and protecting against DNA damage.","authors":"David Díaz, Eva María Baranda-Alonso, Laura Pérez-Revuelta, Juan Felipe Zapata-Acevedo, Frédéric Torossian, Adrienne Anginot, José Ramón Alonso, Marie-Caroline Le Bousse-Kerdilès, Eduardo Weruaga","doi":"10.1177/20417314261442787","DOIUrl":"10.1177/20417314261442787","url":null,"abstract":"<p><p>Cell therapy is a promising strategy for tackling neurodegenerative diseases. The most outstanding results with this approach usually involve neuroprotection of damaged neurons at risk of death, but only with limited success. Current therapies are often based on the idea of \"one gene, one disease, one drug\" for single targets, a concept that limits their actual effectiveness. In contrast, combining different strategies can establish an advanced cell therapy that can slow down neuronal degeneration. In this study, we took advantage of the combination of cell and gene therapy, by transplanting bone marrow stem cells genetically modified to overexpress insulin-like growth factor 1 (IGF1) into a model of selective neurodegeneration, the PCD mouse. This animal is characterized by progressive neuronal loss in the olfactory bulb and alterations in IGF1 levels, among other symptoms. Using different techniques (cell cultures, viral transduction, cell transplants, flow cytometry, qPCR, ELISA, immunohistochemistry, advanced image analysis), our findings showed that neuronal death was virtually blocked, even 130 days after cell transplantation, a result clearly more successful than previous studies. The effects of this transplant are based in part on the regulation of neuroinflammation, increasing the proportion of reactive microglia and reducing that of proinflammatory microglia. In addition, IGF1 overexpression dramatically reduced DNA damage in mutant animals via IGF binding protein 3 pathway: this enhances neuroprotection by complementing the basal effect of cell therapy itself. In summary, our work supports the idea that combining therapeutic approaches and their synergies is a more effective tactic for combating neuronal loss.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261442787"},"PeriodicalIF":10.1,"publicationDate":"2026-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13180132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147973492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibro-adipogenic progenitors enhance functional and structural properties of human 3D tissue engineered skeletal muscles.","authors":"Roy Augustinus, Lotte A de Ridder, Dongxu Zheng, Marnix Franken, Judit Balog, Patrick J van der Vliet, Alessandro Iuliano, Remko Goossens, Johanna I Hamel, W W M Pim Pijnappel, Jessica C de Greef, Silvère M van der Maarel","doi":"10.1177/20417314261441552","DOIUrl":"10.1177/20417314261441552","url":null,"abstract":"<p><p>Human skeletal muscle models often lack important supportive cell types. Here we developed a co-culture three-dimensional tissue engineered skeletal muscle (3D-TESM) model by combining myogenic progenitors (MPs) with genetically-matched immortalized fibro-adipogenic progenitors (iFAPs). FAPs play a crucial physiological role in myogenesis, tissue remodeling and extracellular matrix (ECM) formation. We demonstrate that co-culture 3D-TESMs effectively recapitulate these processes under controlled conditions, thereby enhancing contractile force, muscle tissue integrity and longevity, as well as improving ECM deposition compared to MP-only 3D-TESMs. Moreover, using pro-fibrotic and pro-adipogenic cell culture compositions we were able to mimic pathological features typically observed in muscular dystrophies: excessive ECM production and the formation of fatty infiltrations. This study provides an advanced skeletal muscle model, with enhanced functional and structural properties, capable of recapitulating pathophysiological processes that require FAPs.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261441552"},"PeriodicalIF":10.1,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}