Journal of Tissue Engineering最新文献_第2页

Blood vessels bioengineered from induced pluripotent stem cell derived mesenchymal stem cells and porous silk fibroin coated functional scaffolds. 由诱导多能干细胞衍生的间充质干细胞和多孔丝素包覆的功能支架生物工程血管。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-08-05 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251355723

Luis Larrea Murillo, Zhongda Chen, Jun Song, Adam Mitchel, Steven Woods, Susan J Kimber, Jiashen Li, Yi Li, Tao Wang

{"title":"Blood vessels bioengineered from induced pluripotent stem cell derived mesenchymal stem cells and porous silk fibroin coated functional scaffolds.","authors":"Luis Larrea Murillo, Zhongda Chen, Jun Song, Adam Mitchel, Steven Woods, Susan J Kimber, Jiashen Li, Yi Li, Tao Wang","doi":"10.1177/20417314251355723","DOIUrl":"10.1177/20417314251355723","url":null,"abstract":"The development of small-diameter vascular grafts remains a major challenge in tissue engineering due to limited remodelling and regenerative capabilities. While strides have been made on the biofabrication of vessel mimics, little clinical translation success has been achieved to treat coronary artery disease (CAD). This study aimed to fabricate patient-specific bioengineered vessels using induced pluripotent stem cells (iPSCs) and functionalised biodegradable scaffolds. Human iPSCs were differentiated into mesenchymal stem cells (iMSCs) using SB431542, then further into vascular smooth muscle cells (VSMCs) with PDGF-BB and TGF-β1. Human bone marrow-derived MSCs (hBM-MSCs) were used to optimise differentiation protocols. Electrospun poly-L-lactide (PLLA) scaffolds coated with silk fibroin improved cell adhesion and proliferation. Both hBM-MSCs and iMSCs were seeded on these scaffolds for in-scaffold VSMC differentiation. The resulting cell-laden scaffolds were rolled into tubular structures (~3 mm inner diameter, ~20 mm length). Over 34-36 days, iPSCs differentiated into iMSCs expressing MSC markers (CD73, CD90, CD105), followed by successful VSMC differentiation within 9 days, confirmed by α-SMA, CNN1, SM22, and MYH-11 expression. Silk fibroin-coated PLLA scaffolds enhanced MSC adhesion and proliferation compared to uncoated scaffolds. The engineered tubular grafts displayed VSMC markers and mechanical properties akin to autologous coronary artery bypass grafts (CABGs). This study developed a versatile method to fabricate tissue-engineered blood vessels using stem cells and silk fibroin-coated scaffolds. The resulting grafts exhibited tunica media-like structures and mechanical properties comparable to autografts used in CABG, showing strong potential for clinical application.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251355723"},"PeriodicalIF":7.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From niche to organoid: Engineering bone tissues through microenvironmental insights. 从生态位到类器官：通过微环境洞察工程骨组织。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-07-29 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251358567

Yan Xu, Lingchao Sheng, Minmin Zhu, Zhengcheng He, Xudong Yao, Hongwei Wu

{"title":"From niche to organoid: Engineering bone tissues through microenvironmental insights.","authors":"Yan Xu, Lingchao Sheng, Minmin Zhu, Zhengcheng He, Xudong Yao, Hongwei Wu","doi":"10.1177/20417314251358567","DOIUrl":"10.1177/20417314251358567","url":null,"abstract":"The construction of bone organoids represents a transformative approach in tissue engineering, offering unprecedented opportunities for studying bone biology, disease modeling, and regenerative medicine. The intricate understanding of the skeletal microenvironment, or niche, which governs cellular behavior, tissue organization, and functional maturation, is critical important to construct bone organoid. This review explored insights into the skeletal microenvironment, including the roles of extracellular matrix components, mechanical cues, biochemical signaling, and cellular interactions. It also proposes a foundational strategy how advancements in biomaterials, extracellular matrix, and micro-structure have enabled the precise recapitulation of niche conditions, facilitating the development of physiologically relevant bone organoids. Furthermore, we highlight the applications of these organoids in drug screening, personalized medicine, and bone regeneration. By bridging the gap between niche biology and organoid engineering, this review underscores the potential of microenvironment-driven approaches to revolutionize bone tissue engineering and its translational impact.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251358567"},"PeriodicalIF":7.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GDF15 promotes osteogenic differentiation of human dental pulp stem cells by activating the TGF-β/SMAD signaling pathway. GDF15通过激活TGF-β/SMAD信号通路促进人牙髓干细胞成骨分化。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-07-28 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251357752

Pingmeng Deng, Bin Yang, Chuling Huang, Yuejia Li, Ziyi Mei, Yong Li, Jie Li

{"title":"GDF15 promotes osteogenic differentiation of human dental pulp stem cells by activating the TGF-β/SMAD signaling pathway.","authors":"Pingmeng Deng, Bin Yang, Chuling Huang, Yuejia Li, Ziyi Mei, Yong Li, Jie Li","doi":"10.1177/20417314251357752","DOIUrl":"10.1177/20417314251357752","url":null,"abstract":"Mesenchymal stem cell-mediated bone tissue engineering strategies, including human dental pulp stem cells (hDPSCs), represent an effective therapeutic approach for bone defect repair, particularly in maxillofacial bone defects. Growth differentiation factor 15 (GDF15), a multifunctional cytokine, plays a critical role in bone tissue formation and remodeling. This study aims to investigate the effects of GDF15 on the osteogenic differentiation of hDPSCs and elucidate the underlying molecular mechanisms. Our findings demonstrate that GDF15 expression and secretion are upregulated during the osteogenic differentiation of hDPSCs. Both Gdf15 overexpression and recombinant human GDF15 (rhGDF15) treatment significantly enhanced the osteogenic differentiation of hDPSCs, whereas Gdf15 knockdown produced the opposite effect. In vivo experiments demonstrated that hDPSCs treated with rhGDF15 significantly enhanced new bone formation within implants in both nude mouse subcutaneous transplantation and rat calvarial defect models. Proteomic analysis identified significant enrichment of the TGF-β/SMAD signaling pathway. Molecular docking analysis and co-immunoprecipitation demonstrated the direct binding interaction between GDF15 and TGF-βR2. Both in vitro Western blotting and in vivo immunofluorescence assays confirmed pathway activation. Critically, pharmacological inhibition of this pathway partially reversed the rhGDF15-induced enhancement of osteogenic differentiation in hDPSCs. Collectively, our findings demonstrate that GDF15 promotes osteogenic differentiation of hDPSCs through activation of the TGF-β/SMAD signaling pathway, thereby proposing a novel therapeutic strategy for bone repair and regenerative treatment.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251357752"},"PeriodicalIF":7.0,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro models of muscle spindles: From traditional methods to 3D bioprinting strategies. 肌肉纺锤体的体外模型：从传统方法到3D生物打印策略。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-07-23 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251343388

Yuannan Kang, Deepak M Kalaskar, Darren J Player

{"title":"In vitro models of muscle spindles: From traditional methods to 3D bioprinting strategies.","authors":"Yuannan Kang, Deepak M Kalaskar, Darren J Player","doi":"10.1177/20417314251343388","DOIUrl":"10.1177/20417314251343388","url":null,"abstract":"Muscle spindles are key proprioceptive mechanoreceptors composed of intrafusal fibres that regulate kinaesthetic sensations and reflex actions. Traumatic injuries and neuromuscular diseases can severely impair the proprioceptive feedback, yet the regenerative potential and cell-matrix interactions of muscle spindles remain poorly understood. There is a pressing need for robust tissue-engineered models to study spindle development, function and regeneration. Traditional approaches, while insightful, often lack physiological relevance and scalability. Three-dimensional (3D) bioprinting offers a promising approach to fabricate biomimetic, scalable, and animal-free muscle spindle constructs with controlled cellular architecture. Various bioprinting techniques - including inkjet, extrusion, digital light projection and laser-assisted bioprinting - have been explored for skeletal muscle fabrication, but replicating intrafusal fibre complexity remains a challenge. A major challenge lies in bioink development, where biocompatibility, printability and mechanical strength must be balanced to support intrafusal fibre differentiation and proprioceptive function. Recent molecular insights into spindle anatomy, innervation and extracellular matrix composition are shaping biofabrication strategies. This review discusses the current state of muscle spindle modelling, the application of 3D bioprinting in intrafusal fibre engineering, key challenges and future directions.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251343388"},"PeriodicalIF":7.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Estradiol-17β [E2] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model. 缩回：雌二醇-17β [E2]在体外组织工程三维阴道伤口模型中刺激伤口愈合。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-07-09 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251358965

引用次数: 0

Advances in biomaterial-based composite spheroid for articular cartilage regeneration. 生物材料基复合球体关节软骨再生研究进展。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-07-01 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251349669

Nopphadol Udomluck, Hansoo Park, Jae Young Lee

{"title":"Advances in biomaterial-based composite spheroid for articular cartilage regeneration.","authors":"Nopphadol Udomluck, Hansoo Park, Jae Young Lee","doi":"10.1177/20417314251349669","DOIUrl":"10.1177/20417314251349669","url":null,"abstract":"Articular cartilage plays a crucial role in reducing friction between bones and enabling movements; however, it is frequently degraded due to persistent joint stress, aging, and osteoarthritis. As its self-repair ability is limited, various cell-based therapeutic strategies have been developed for cartilage regeneration. Conventional two-dimensional (2D) cell cultures inadequately replicate the complex intercellular interactions of native cartilage. In contrast, three-dimensional (3D) cell spheroid cultures can more accurately mimic in vivo cellular physiology, offering superior regenerative potential via improved cell-cell and cell-matrix interactions. These interactions can be enhanced with biomaterials to form composite spheroids, which exhibit substantial potential for improving cartilage regeneration and attenuating osteoarthritis progression in vivo by promoting cell survival and tissue integration. This review highlights current strategies for developing biomimetic composite spheroid systems, including spheroid encapsulation, scaffold incorporation, and 3D bioprinting. Furthermore, we discuss their advantages, translational potential for in vivo cartilage repair, and the challenges and future directions in cartilage tissue engineering.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251349669"},"PeriodicalIF":6.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced cell sheet engineering through combination of single cells and spheroids on liquid interface using perfluorocarbon. 通过使用全氟碳在液体界面上结合单细胞和球体来增强细胞片工程。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-06-29 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251350316

Ji Hye Park, Ji-Seok Han, Eun-Jung Ann, Cho Yeon Kim, Byoung-Seok Lee, Ji Su Kang, Sun-Sook Song, Junhee Lee, Sun-Woong Kang

{"title":"Enhanced cell sheet engineering through combination of single cells and spheroids on liquid interface using perfluorocarbon.","authors":"Ji Hye Park, Ji-Seok Han, Eun-Jung Ann, Cho Yeon Kim, Byoung-Seok Lee, Ji Su Kang, Sun-Sook Song, Junhee Lee, Sun-Woong Kang","doi":"10.1177/20417314251350316","DOIUrl":"10.1177/20417314251350316","url":null,"abstract":"Cell sheet engineering provides a scaffold-free strategy for fabricating cohesive tissue constructs, but challenges remain in maintaining structural integrity and mimicking complex tissue architectures. This study demonstrated perfluorodecalin-based liquid-liquid interfaces, known for their inertness and stability, as a simple, and efficient platform for fabricating cell sheets. Using single cells, spheroids, and their combination, we evaluated methods to enhance sheet formation. Single cells formed cohesive sheets at high densities (4 × 106 cells/well) but exhibited limited long-term stability due to nutrient constraints. Spheroids formed robust sheets at lower densities (2 × 106 cells/well), whereas higher densities impaired fusion. The mixed approach combined the advantages of both, improving uniformity, mechanical stability, and spheroid fusion, while mimicking muscle-like structures with vascular networks. Additionally, the cell sheets retained adipogenic and chondrogenic differentiation potential, highlighting their functional viability. These findings establish liquid interfaces as a practical and versatile platform for tissue engineering, regenerative medicine, and in vitro modeling.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251350316"},"PeriodicalIF":6.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive characterization of cell and tissue responses toward high hydrostatic pressure treatment: Molecular feedback and structural integrity in bone graft processing. 细胞和组织对高静水压力处理反应的综合表征：骨移植过程中的分子反馈和结构完整性。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-06-28 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251337193

Henrike Loeffler, Jan-Oliver Sass, Lorena Muelders, Julian Bauer, Oliver Friedrich, Rainer Bader, Annett Klinder, Janine Waletzko-Hellwig

{"title":"Comprehensive characterization of cell and tissue responses toward high hydrostatic pressure treatment: Molecular feedback and structural integrity in bone graft processing.","authors":"Henrike Loeffler, Jan-Oliver Sass, Lorena Muelders, Julian Bauer, Oliver Friedrich, Rainer Bader, Annett Klinder, Janine Waletzko-Hellwig","doi":"10.1177/20417314251337193","DOIUrl":"10.1177/20417314251337193","url":null,"abstract":"In the field of tissue reconstruction, the development and improvement of suitable bone grafts is of increasing importance. The implementation of bone banks enables the international distribution of suitable allografts that can be used for defect reconstruction. Currently used procedures have significant drawbacks, especially regarding biomechanical and structural properties. These can be overcome by using the technique of high hydrostatic pressure (HHP) processing. To date, little is known about the impact of HHP protocol alterations including pressure-transmitting medium or temperature regarding bone graft integrity. Data of the present study show that a low-temperature and medium-pressure treatment using isotonic sodium chloride solution as the pressure-transmitting medium generated devitalized bone tissue with preserved extracellular matrix. Specifically, efficient devitalization of human primary osteoblasts (hOBs) was found starting from 150 MPa with cell death being a complex interaction between different mechanisms. Furthermore, protein denaturation in response to HHP treatment that was predominantly observed at 600 MPa led to non-significant impairment of biomechanical properties. Effects of HHP treatment on the bone tissue did not lead to any noticeable compromise in biocompatibility. Accordingly, the presented protocol may be applied as a medical device to improve the outcome of patients undergoing bone defect reconstruction with allogenic grafts.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251337193"},"PeriodicalIF":6.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in hydrogels for capturing and neutralizing inflammatory cytokines. 水凝胶捕获和中和炎症细胞因子的研究进展。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-06-25 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251342175

Hongwei Qin, Ze Li, Sicheng Li, Jinjian Huang, Jianan Ren, Xiuwen Wu

{"title":"Advances in hydrogels for capturing and neutralizing inflammatory cytokines.","authors":"Hongwei Qin, Ze Li, Sicheng Li, Jinjian Huang, Jianan Ren, Xiuwen Wu","doi":"10.1177/20417314251342175","DOIUrl":"10.1177/20417314251342175","url":null,"abstract":"Inflammatory cytokines play a crucial role in the inflammatory response, and their aberrant expression and overproduction are closely associated with the development of many diseases. However, traditional inflammation treatment strategies are often accompanied by serious side effects, limiting their widespread use. In recent years, hydrogel, as a material with a three-dimensional network structure, good biocompatibility and modulability, has great potential for trapping and neutralizing inflammatory factors. Hydrogels can capture and neutralize inflammatory cytokines through various mechanisms such as electrostatic interactions, coupling with cytokine antibodies or binding nanoparticles. In addition, hydrogel microspheres, an important form of hydrogels, have excellent broad-spectrum binding of inflammatory cytokines in combination schemes with cell membranes. This article reviews recent research advances in hydrogel capture and neutralization of inflammatory cytokines, discussing the advantages of various mechanisms and their applications in different diseases. Overall, we believe that hydrogels are expected to further advance the development of therapeutic modalities for inflammatory diseases in the future.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251342175"},"PeriodicalIF":6.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity. GelMA中缺乏生化信号导致肠道类器官的极性逆转，不依赖于机械互易性。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-06-13 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251345000

Lenie Vanhove, Thomas Van Gansbeke, Bert Devriendt, Ruben Van der Meeren, Ruslan I Dmitriev, Irina A Okkelman

{"title":"Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity.","authors":"Lenie Vanhove, Thomas Van Gansbeke, Bert Devriendt, Ruben Van der Meeren, Ruslan I Dmitriev, Irina A Okkelman","doi":"10.1177/20417314251345000","DOIUrl":"10.1177/20417314251345000","url":null,"abstract":"Xenogeneic tumour origin and batch-to-batch variability of Engelbreth-Holm-Swarm sarcoma tumour cell-derived hydrogels (Matrigel, Cultrex) limit the biomedical application of organoids in tissue engineering. The gelatin-methacryloyl (GelMA) hydrogels represent a defined, tunable, and GMP-friendly alternative, but they are rarely studied as alternative to Matrigel. Here, we studied effects of mechanical properties of GelMA and addition of laminin-111 on encapsulation and growth of small intestinal organoids. GelMA-embedded organoids displayed polarity reversion, resulting in apical-out and apical-basal phenotypes, independent from the matrix stiffness. Addition of laminin-111 softened hydrogels and also resulted in a partial restoration of the basal-out phenotype. Interestingly, despite the incomplete polarity restoration, GelMA-organoids still showed minor growth. GelMA stiffness and concentration influenced the transition from 3D to 2D organoid cultures. Collectively, our study confirms that tuning of GelMA mechanical properties alone cannot recapitulate the basal membrane matrix. However, controlled polarity reversion offers a tool for engineering organoids and enabling apical membrane access.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251345000"},"PeriodicalIF":6.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0