Journal of Tissue Engineering最新文献

{"title":"Integrating single-cell and spheroid strategies in tissue engineering: Comparative insights from pancreas, cartilage, heart and brain.","authors":"Shankar Shanmuga Sundaram, Michelle Min Shuen Tan, Quang Bach Le, Wei Seong Toh, Deepak Choudhury","doi":"10.1177/20417314261460410","DOIUrl":"10.1177/20417314261460410","url":null,"abstract":"<p><p>Tissue engineering (TE) remains a cornerstone of regenerative medicine, aiming to bypass the limitation of organ transplantation through the fabrication of functional tissue substitutes. Traditionally, TE has followed two primary paradigms: the top-down approach, utilising single cells seeded on a scaffold, and the bottom-up approach, employing cell spheroids as building blocks. While top-down offers architectural and structural control, bottom-up promotes self-assembly, native-like extracellular matrix deposition, and intercellular signalling. However, modern techniques increasingly blur this dichotomy, creating a spectrum of cell-based fabrication approaches. This review evaluates the diverse approaches across four major tissue classes: epithelial (pancreas as an example), connective (cartilage), muscle (heart), and nervous (brain) tissues. For each tissue, we examine notable studies to evaluate how different assembly methods recapitulate native tissue properties. By reviewing case studies across diverse tissue types, we highlight the relative strengths and limitations of various fabrication strategies. Although this review is limited by a selective cross-section of literature within a rapidly advancing technological landscape, it provides critical insights into optimising next-generation tissue constructs. In conclusion, we posit that there is no universal fabrication strategy; rather, the future of the field depends on tailoring approaches along this single-cell-to-spheroid spectrum based on the specific architectural and functional demands of the target tissue.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261460410"},"PeriodicalIF":10.1,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13392336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148578943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the memory of the extracellular matrix using MASH-derived decellularized scaffolds.","authors":"Gabriel Reis Pinto, Luana Diniz Guerra Braz, Yasmin Pestana, Alexandre Cerqueira da Silva Filho, Giulia Roldão B Freire, Maria Isabel Moraes do Amaral Candido Gomes, Julia Helena Oliveira de Barros, Thamires Siqueira de Oliveira, Isadora Z L F Feng, Barbara Fidelix Santana, Hernandes F Carvalho, Cherley Borba Vieira Andrade, Lucas Pires Guarnier, Érica Almeida Amorim, Cibele Ferreira Pimentel, Alfredo Miranda de Goes, M Fátima Leite, Robson A S Santos, Marina Amaral Alves, Regina Coeli Dos Santos Goldenberg, Marlon Lemos Dias","doi":"10.1177/20417314261468885","DOIUrl":"10.1177/20417314261468885","url":null,"abstract":"<p><p>Emerging evidence suggests that the extracellular matrix (ECM) possesses a \"memory\" that can influence cell physiology and recellularization outcomes. Understanding this memory is essential to allow the use of bioengineered organs derived from diseased ECM, offering a solution to the critical organ shortage. To address this, we investigated whether the memory of ECM derived from metabolic dysfunction-associated steatohepatitis (MASH) livers impacts disease establishment following transplantation. Partial orthotopic transplantation of decellularized MASH-derived ECM was performed in control and MASH recipients. Histological analysis confirmed complete recellularization; however, molecular and metabolomic analyses revealed that MASH ECM stimulated de novo lipogenesis and fibrogenesis, inducing impaired lipid oxidation and mitochondrial dysfunction, which contributed to disease progression by promoting altered lipid turnover and inflammatory signalling. In vitro analysis revealed that MASH-ECM disrupted calcium signalling and promoted the maintenance of a pathological phenotype. Although derived from diseased livers, human ECM can promote cell survival and permissiveness. In conclusion, diseased ECM memory impacts cell physiology, suggesting that the scaffold can drive disease progression independently of the cellular environment. Thus, further studies are needed to develop strategies capable of reversing the pathological memory associated with ECM to allow its use in liver transplantation.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261468885"},"PeriodicalIF":10.1,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13380698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Wnt/BMP Co-activation accelerates osteogenic differentiation of human pluripotent stem cells via paraxial mesoderm induction.","authors":"Hongsik Kim, Sujin Kim, A-Hyeon Kim, Hamyoung Lee, Seongwoo Jeong, Hayoun Kim, Jungbum Kim, Wonhwa Lee, Hwan Drew Kim","doi":"10.1177/20417314261468879","DOIUrl":"10.1177/20417314261468879","url":null,"abstract":"<p><p>Bone healing is a complex and well-organized process, regulated by various factors ranging from growth factors to hormones, cytokines, mechanical stimuli, and aging. Recently, numerous techniques have been devised to efficiently induce the differentiation of human induced pluripotent stem cells (hiPSCs) to osteoblasts. However, enhancing the efficiency of osteoblast differentiation remains a challenge. Thus, we induced the differentiation of hiPSCs to mesodermal cells through Wnt/BMP signaling based on the generation of hiPSCs. After successful generation of hiPSCs, we induced the differentiation of mesodermal cells to osteoblasts. The results revealed that the runt-related transcription factor 2 (<i>RUNX2</i>)-encoding gene was upregulated from the early differentiation stage; hence, the expression of the mature osteoblast marker was higher compared to that observed in other differentiation stages. In addition, the deposition of substrates in mature bones was observed. The results were confirmed via real-time PCR, Alizarin Red Staining, and Von Kossa staining. The coactivation of Wnt and BMP signaling was shown to rapidly and effectively promote the differentiation of osteoblasts. The findings of this study will provide a foundation for future studies on the mechanism of osteoblast development, as well as the biological and pathological investigations of drug screening and bone regeneration tracing.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261468879"},"PeriodicalIF":10.1,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13369415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148456201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-engineered blood vessels for clinical translation: Design logic, representative advances and persistent barriers.","authors":"Junjie Chen, Jiayang He, Dujiang Yang, Yuhan He, Junhao Zhang, Chunshui He, Wei Zeng","doi":"10.1177/20417314261466954","DOIUrl":"10.1177/20417314261466954","url":null,"abstract":"<p><p>Tissue-engineered blood vessels are promising alternatives for small-diameter vascular reconstruction, but clinical translation remains limited by thrombosis, compliance mismatch, delayed endothelialization and unstable host remodeling. This review synthesizes representative advances in scaffold design, seed-cell selection, bioactive regulation and biofabrication, emphasizing how these components interact under hemodynamic load. Rather than cataloguing technologies, we argue that translational success depends on matching material architecture, cellular phenotype, immune remodeling and manufacturing constraints to specific clinical indications. Acellular or host-repopulating grafts may be most realistic for urgent trauma or vascular access, whereas coronary and distal peripheral reconstruction require tighter control of endothelialization and mechanics. Future tissue-engineered vessels should be evaluated as indication-specific products with clinically meaningful benchmarks beyond short-term patency.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261466954"},"PeriodicalIF":10.1,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13342367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism-guided biomaterial strategies for intervertebral disc degeneration: Pathological heterogeneity, functional classification, and translational perspectives.","authors":"Jianpeng Chen, Wenhao Deng, Shiran Zhou, Jun Yan, Kai Chen, Qianliang Wang, Shujun Lv","doi":"10.1177/20417314261464764","DOIUrl":"10.1177/20417314261464764","url":null,"abstract":"<p><p>Intervertebral disc degeneration (IVDD) is a multifactorial and clinically heterogeneous condition involving disturbances in oxidative balance, mitochondrial homeostasis, inflammatory signaling, susceptibility to regulated cell death, extracellular matrix integrity, and biomechanical function. Although biomaterial-based strategies have shown significant promise in preclinical studies, their clinical translation remains hindered by pathological heterogeneity, insufficient mechanistic validation, and frequent reliance on simplified experimental models. These models often fail to fully replicate the chronic, mechanically complex, and clinically diverse nature of human disc degeneration. In addition, current biomaterial strategies are commonly classified according to material composition or isolated molecular targets, potentially obscuring their functional objectives and limiting mechanistic comparisons across therapeutic platforms. This review presents a mechanism-guided framework for interpreting biomaterial interventions in IVDD. Biomaterial strategies are discussed based on their primary pathological targets and therapeutic intentions, including inflammatory regulation, restoration of redox homeostasis, mitochondrial protection, ferroptosis modulation, extracellular matrix preservation, and multifunctional microenvironment-responsive interventions. This framework acknowledges that these pathological processes are interconnected and vary in their relative dominance across clinical and pathological contexts and patient populations. We further analyze current biomaterial strategies according to mechanistic intervention layers, including upstream sensing and initiation-level control, restraint of intracellular amplification, organelle stabilization, regulation of execution checkpoints, and integration with higher-order structural organization. This layered perspective emphasizes that the efficacy of biomaterials depends not only on their composition but also on the pathological context, depth of regulatory influence, mechanistic specificity, and compatibility across intervention layers. Collectively, this review provides an integrated, mechanism-oriented framework for biomaterial design, preclinical evaluation, and translational development in IVDD therapy.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261464764"},"PeriodicalIF":10.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13323677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering next-generation organoids: A review on bioprinting strategies, bioink innovations, and frontier applications.","authors":"Defeng Sun, Xuemei Fan, Hua Sun, Dongmei Li, Ruijia Shen, Pu Xia, Zhenying Zhao","doi":"10.1177/20417314261459941","DOIUrl":"10.1177/20417314261459941","url":null,"abstract":"<p><p>While organoids hold immense promise as in vitro three-dimensional (3D) models, their translational utility is fundamentally constrained by passive diffusion limits (>800 μm), which inevitably trigger necrotic core formation and stochastic structural heterogeneity. This review elucidates how bioprinting shatters these physical bottlenecks by executing a paradigm shift toward spatiotemporal determinism. We systematically decode the mechanobiological evolution of bioinks-charting the transition from exogenous static matrices, which now function as temporal controllers via tunable stress relaxation to direct YAP/TAZ mechanotransduction, to the emerging paradigm of \"engineerable living bioinks\" driven by endogenous, cadherin-mediated fluid-to-solid jamming transitions. Furthermore, we critically evaluate frontier spatial strategies, highlighting how sacrificial networks and deterministic multi-material assembly establish active convective infrastructures and precise biophysical boundary conditions. By enforcing this rigorous baseline, these technologies definitively rectify pharmacokinetic/pharmacodynamic (PK/PD) distortions-eradicating false-positive noise in high-throughput screening and bridging the post-implantation mass transport vacuum-elevating organoids from stochastic clusters to highly predictive pathophysiological macro-models. Ultimately, we posit that transitioning from isolated morphological fabrication to resolving the inherent systemic metabolic paradoxes of multi-lineage integration is the absolute prerequisite for clinical translation.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261459941"},"PeriodicalIF":10.1,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13263509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical regulation of mesenchymal stem cell osteogenesis, bone matrix homoeostasis, and skeletal pathology.","authors":"Udipt R Das, Hussain Jaffery, Penelope M Tsimbouri, Matthew J Dalby","doi":"10.1177/20417314261454194","DOIUrl":"10.1177/20417314261454194","url":null,"abstract":"<p><p>Osteogenesis is the process by which mesenchymal stem/stromal cells (MSCs) differentiate into mature osteoblasts, forming a mineralised bone matrix. This process is regulated by soluble factors, mechanical stimuli, and the extracellular matrix (ECM), which together maintain bone and mineral homoeostasis. Mechanotransduction, the conversion of physical cues into intracellular signals, is crucial for MSC fate determination and orchestrates bone matrix remodelling, balancing formation and resorption. Continuous mechanical loading supports optimal osteogenic differentiation, whereas mechanical unloading (sub-physiological mechanical loading) disrupts this equilibrium and increases bone loss risk. These processes involve complex crosstalk among local and systemic factors, immune cells, and osteoblast-osteoclast interactions in response to mechanical cues. This review discusses key biomechanical factors regulating MSC osteogenic differentiation and bone remodelling, and synthesises evidence on skeletal immobilisation and other unloading-associated conditions that contribute to skeletal anomalies. It further emphasises nanovibrational stimulation as a novel approach to enhance MSC osteogenesis and mitigate skeletal anomalies.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261454194"},"PeriodicalIF":10.1,"publicationDate":"2026-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13237463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondrocyte metabolic transition from proliferation to quiescence revealed by FLIM in postnatal mouse knee joints.","authors":"Nadezda Ignatyeva, Boris Yakimov, Anastasiia D Kurenkova, Irina A Romanova, Pavel D Kibirskiy, Nikita Gavrilov, Anastasiya Patsyurkevich, Irina D Shcherbakova, Artem M Mozherov, Aleksandra V Kashina, Evgeny A Shirshin, Peter S Timashev, Ekaterina V Medvedeva","doi":"10.1177/20417314261432891","DOIUrl":"10.1177/20417314261432891","url":null,"abstract":"<p><p>Adult articular cartilage chondrocytes have a limited capacity to divide compared to juvenile cells, but the mechanisms behind this decline remain unclear. This study investigates metabolic changes associated with the cessation of chondrocyte proliferation in mouse articular cartilage. Using 5-ethynyl-2'-deoxyuridine (EdU) labeling, the postnatal decline in proliferation was tracked. Label-free fluorescence-lifetime imaging microscopy (FLIM) method, combined with artificial intelligence (AI)-assisted image segmentation, was applied to live cartilage sections to analyze metabolic parameters. Results showed that 1-month-old articular cartilage chondrocytes enter quiescence with significant changes in FLIM fluorescence decay parameters across cartilage zones compared to juvenile chondrocytes. Chondroprogenitors in the superficial zone showed a gradual decrease in citrate synthase content, while glycolytic activity increased with tissue depth. These findings reveal metabolic reprogramming that enables chondrocytes to adapt their metabolism despite limited oxygen availability to meet functional demands. Investigating these chondrocyte adaptations provides key insights for identifying metabolic targets and improving the design of durable, well-integrated tissue-engineered cartilage.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261432891"},"PeriodicalIF":10.1,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13198642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-free therapy for alopecia via the secretome of hiPSC-derived dermal papilla cells.","authors":"Hyesoo Hwangbo, Aoyang Pu, Wanyu Tan, Eunice Dotse, Huanhuan Sun, Xin Gan, Yun-Gwi Park, Yimin Lai, Soon-Jung Park, Inho Choi, In-Rok Oh, Kwan Ting Chow, Sung-Hwan Moon, Hae-Won Kim, Byung Cheol Park, Kiwon Ban","doi":"10.1177/20417314261449597","DOIUrl":"10.1177/20417314261449597","url":null,"abstract":"<p><p>Alopecia is highly prevalent and debilitating, yet current drugs provide limited, reversible benefit with notable side effects. We established a rapid protocol to generate human induced pluripotent stem cell-derived dermal papilla cells (hiPSC-DPCs) and demonstrated that their conditioned medium (CM) acts as a potent, cell-free hair-regenerative therapy. Transdermal delivery of hiPSC-DPCs or CM accelerated anagen re-entry and hair regrowth in depilated mice, and hiPSC-DPC CM outperformed minoxidil in promoting ex vivo hair-shaft elongation and in vitro proliferation and migration of primary DPCs and keratinocytes. Proteomic and metabolomic profiling revealed enrichment of growth factors, antioxidants, and immunomodulatory metabolites linked to TNF and PI3K-Akt signaling, conferring superior anti-inflammatory and cytoprotective properties relative to primary DPC CM. Moreover, hiPSC-DPC CM mitigated dihydrotestosterone (DHT)-induced pathology by suppressing androgen receptor expression and nuclear translocation. These findings position hiPSC-DPC secretome as a dual-functional, regenerative, and anti-androgenic biologic with translational potential for durable alopecia treatment.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261449597"},"PeriodicalIF":10.1,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13191134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold-stimulated browning graft fat enhances burn wound recovery through accelerating DAMP-ADSC activation.","authors":"Wenhui Ma, Zhenyu Quan, Li Jiang, Pengya An, Qi Zhao, Yucheng Luo, Yueqi Zhang, Xiaohua Feng, Yong Pan","doi":"10.1177/20417314261451461","DOIUrl":"10.1177/20417314261451461","url":null,"abstract":"<p><p>Suboptimal skin regeneration in patients with severe burns leads to significant trauma. Due to their favorable biological properties, fat grafts have been widely used in wound repair. The present study aimed to investigate the regenerative benefits of cold-stimulated fat graft in a contact burn model using C57BL/6J mice. Transplantation of browning fat grafts, with enhanced adipogenic capacity and decreased fibrosis, effectively promoted granulation tissue thickness and re-epithelialization areas. Cold-stimulated fat graft had a significant accumulation of ADSCs, which migrated into wound skin. In adipocyte-deficient mice, the impaired wound-healing phenotype was reversed by browning fat graft. Cold-stimulated fat also exhibited higher levels of damage-associated molecular patterns (DAMPs), which induced the proliferation of ADSCs and promoted ADSC proliferation and differentiation into mature adipocytes. Inhibition of DAMP-related signaling abolished the repair benefits of browning fat graft. In conclusion, transplantation of cold-stimulated fat represents a highly effective strategy for treating burn wounds through promoting DAMP signaling and dermal adipose remodeling.</p>","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261451461"},"PeriodicalIF":10.1,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13187421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}