Journal of Tissue Engineering最新文献

Enhanced cell sheet engineering through combination of single cells and spheroids on liquid interface using perfluorocarbon. 通过使用全氟碳在液体界面上结合单细胞和球体来增强细胞片工程。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-06-29 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251350316

Ji Hye Park, Ji-Seok Han, Eun-Jung Ann, Cho Yeon Kim, Byoung-Seok Lee, Ji Su Kang, Sun-Sook Song, Junhee Lee, Sun-Woong Kang

{"title":"Enhanced cell sheet engineering through combination of single cells and spheroids on liquid interface using perfluorocarbon.","authors":"Ji Hye Park, Ji-Seok Han, Eun-Jung Ann, Cho Yeon Kim, Byoung-Seok Lee, Ji Su Kang, Sun-Sook Song, Junhee Lee, Sun-Woong Kang","doi":"10.1177/20417314251350316","DOIUrl":"10.1177/20417314251350316","url":null,"abstract":"Cell sheet engineering provides a scaffold-free strategy for fabricating cohesive tissue constructs, but challenges remain in maintaining structural integrity and mimicking complex tissue architectures. This study demonstrated perfluorodecalin-based liquid-liquid interfaces, known for their inertness and stability, as a simple, and efficient platform for fabricating cell sheets. Using single cells, spheroids, and their combination, we evaluated methods to enhance sheet formation. Single cells formed cohesive sheets at high densities (4 × 106 cells/well) but exhibited limited long-term stability due to nutrient constraints. Spheroids formed robust sheets at lower densities (2 × 106 cells/well), whereas higher densities impaired fusion. The mixed approach combined the advantages of both, improving uniformity, mechanical stability, and spheroid fusion, while mimicking muscle-like structures with vascular networks. Additionally, the cell sheets retained adipogenic and chondrogenic differentiation potential, highlighting their functional viability. These findings establish liquid interfaces as a practical and versatile platform for tissue engineering, regenerative medicine, and in vitro modeling.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251350316"},"PeriodicalIF":6.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive characterization of cell and tissue responses toward high hydrostatic pressure treatment: Molecular feedback and structural integrity in bone graft processing. 细胞和组织对高静水压力处理反应的综合表征：骨移植过程中的分子反馈和结构完整性。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-06-28 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251337193

Henrike Loeffler, Jan-Oliver Sass, Lorena Muelders, Julian Bauer, Oliver Friedrich, Rainer Bader, Annett Klinder, Janine Waletzko-Hellwig

{"title":"Comprehensive characterization of cell and tissue responses toward high hydrostatic pressure treatment: Molecular feedback and structural integrity in bone graft processing.","authors":"Henrike Loeffler, Jan-Oliver Sass, Lorena Muelders, Julian Bauer, Oliver Friedrich, Rainer Bader, Annett Klinder, Janine Waletzko-Hellwig","doi":"10.1177/20417314251337193","DOIUrl":"10.1177/20417314251337193","url":null,"abstract":"In the field of tissue reconstruction, the development and improvement of suitable bone grafts is of increasing importance. The implementation of bone banks enables the international distribution of suitable allografts that can be used for defect reconstruction. Currently used procedures have significant drawbacks, especially regarding biomechanical and structural properties. These can be overcome by using the technique of high hydrostatic pressure (HHP) processing. To date, little is known about the impact of HHP protocol alterations including pressure-transmitting medium or temperature regarding bone graft integrity. Data of the present study show that a low-temperature and medium-pressure treatment using isotonic sodium chloride solution as the pressure-transmitting medium generated devitalized bone tissue with preserved extracellular matrix. Specifically, efficient devitalization of human primary osteoblasts (hOBs) was found starting from 150 MPa with cell death being a complex interaction between different mechanisms. Furthermore, protein denaturation in response to HHP treatment that was predominantly observed at 600 MPa led to non-significant impairment of biomechanical properties. Effects of HHP treatment on the bone tissue did not lead to any noticeable compromise in biocompatibility. Accordingly, the presented protocol may be applied as a medical device to improve the outcome of patients undergoing bone defect reconstruction with allogenic grafts.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251337193"},"PeriodicalIF":6.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in hydrogels for capturing and neutralizing inflammatory cytokines. 水凝胶捕获和中和炎症细胞因子的研究进展。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-06-25 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251342175

Hongwei Qin, Ze Li, Sicheng Li, Jinjian Huang, Jianan Ren, Xiuwen Wu

{"title":"Advances in hydrogels for capturing and neutralizing inflammatory cytokines.","authors":"Hongwei Qin, Ze Li, Sicheng Li, Jinjian Huang, Jianan Ren, Xiuwen Wu","doi":"10.1177/20417314251342175","DOIUrl":"10.1177/20417314251342175","url":null,"abstract":"Inflammatory cytokines play a crucial role in the inflammatory response, and their aberrant expression and overproduction are closely associated with the development of many diseases. However, traditional inflammation treatment strategies are often accompanied by serious side effects, limiting their widespread use. In recent years, hydrogel, as a material with a three-dimensional network structure, good biocompatibility and modulability, has great potential for trapping and neutralizing inflammatory factors. Hydrogels can capture and neutralize inflammatory cytokines through various mechanisms such as electrostatic interactions, coupling with cytokine antibodies or binding nanoparticles. In addition, hydrogel microspheres, an important form of hydrogels, have excellent broad-spectrum binding of inflammatory cytokines in combination schemes with cell membranes. This article reviews recent research advances in hydrogel capture and neutralization of inflammatory cytokines, discussing the advantages of various mechanisms and their applications in different diseases. Overall, we believe that hydrogels are expected to further advance the development of therapeutic modalities for inflammatory diseases in the future.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251342175"},"PeriodicalIF":6.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity. GelMA中缺乏生化信号导致肠道类器官的极性逆转，不依赖于机械互易性。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-06-13 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251345000

Lenie Vanhove, Thomas Van Gansbeke, Bert Devriendt, Ruben Van der Meeren, Ruslan I Dmitriev, Irina A Okkelman

{"title":"Lack of biochemical signalling in GelMA leads to polarity reversion in intestinal organoids independent from mechanoreciprocity.","authors":"Lenie Vanhove, Thomas Van Gansbeke, Bert Devriendt, Ruben Van der Meeren, Ruslan I Dmitriev, Irina A Okkelman","doi":"10.1177/20417314251345000","DOIUrl":"10.1177/20417314251345000","url":null,"abstract":"Xenogeneic tumour origin and batch-to-batch variability of Engelbreth-Holm-Swarm sarcoma tumour cell-derived hydrogels (Matrigel, Cultrex) limit the biomedical application of organoids in tissue engineering. The gelatin-methacryloyl (GelMA) hydrogels represent a defined, tunable, and GMP-friendly alternative, but they are rarely studied as alternative to Matrigel. Here, we studied effects of mechanical properties of GelMA and addition of laminin-111 on encapsulation and growth of small intestinal organoids. GelMA-embedded organoids displayed polarity reversion, resulting in apical-out and apical-basal phenotypes, independent from the matrix stiffness. Addition of laminin-111 softened hydrogels and also resulted in a partial restoration of the basal-out phenotype. Interestingly, despite the incomplete polarity restoration, GelMA-organoids still showed minor growth. GelMA stiffness and concentration influenced the transition from 3D to 2D organoid cultures. Collectively, our study confirms that tuning of GelMA mechanical properties alone cannot recapitulate the basal membrane matrix. However, controlled polarity reversion offers a tool for engineering organoids and enabling apical membrane access.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251345000"},"PeriodicalIF":6.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a reliable method for human triple-negative breast cancer organotypic culture: Improving imaging and genomic studies in 3D cultures. 开发一种可靠的人类三阴性乳腺癌器官型培养方法：改进三维培养的成像和基因组研究。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-05-07 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251326668

Mercedes Olvera-Valencia, Verónica Garcia-Castillo, Rosalío Ramos-Payan, Maribel Aguilar-Medina, Samuel Trujano-Camacho, Alejandro López-Saavedra, Laurence A Marchat, Cesar López-Camarillo, Ronen Sumagin, Eloy Pérez-Yepez, Carlos Pérez-Plasencia

{"title":"Development of a reliable method for human triple-negative breast cancer organotypic culture: Improving imaging and genomic studies in 3D cultures.","authors":"Mercedes Olvera-Valencia, Verónica Garcia-Castillo, Rosalío Ramos-Payan, Maribel Aguilar-Medina, Samuel Trujano-Camacho, Alejandro López-Saavedra, Laurence A Marchat, Cesar López-Camarillo, Ronen Sumagin, Eloy Pérez-Yepez, Carlos Pérez-Plasencia","doi":"10.1177/20417314251326668","DOIUrl":"https://doi.org/10.1177/20417314251326668","url":null,"abstract":"Triple-negative breast cancer (TNBC) is highly aggressive and lacks targeted therapies, posing a major challenge in oncology. Traditional two-dimensional (2D) cell cultures fail to capture the tumor microenvironment's complexity, whereas three-dimensional (3D) cultures provide a more accurate model of tumor biology. We developed an advanced 3D culture system for TNBC cell lines BT-20 and MDA-MB-231, enhancing the hanging-drop method with Matrigel to restore essential extracellular matrix interactions. Confocal imaging showed MDA-MB-231 cells forming clusters typical of aggressive carcinoma, while BT-20 cells organized into duct-like structures. Molecular analysis of PI3K and β-catenin target genes revealed distinct expression patterns, with PI3K overexpressed and β-catenin downregulated in 3D cultures. Moreover, β-catenin distribution in the 3D cell culture closely resembles its pattern in tissue. These findings underscore the value of 3D models in understanding TNBC progression and in supporting the exploration of novel therapeutic strategies.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251326668"},"PeriodicalIF":6.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances. ipsc衍生免疫皮肤模型的建立，用于皮肤致敏物质的鉴定。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-05-06 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251336296

Marla Dubau, Tarada Tripetchr, Lava Mahmoud, Fabian Schumacher, Burkhard Kleuser

{"title":"Development of an iPSC-derived immunocompetent skin model for identification of skin sensitizing substances.","authors":"Marla Dubau, Tarada Tripetchr, Lava Mahmoud, Fabian Schumacher, Burkhard Kleuser","doi":"10.1177/20417314251336296","DOIUrl":"https://doi.org/10.1177/20417314251336296","url":null,"abstract":"The development of immunocompetent skin models marks a significant advancement in in vitro methods for detecting skin sensitizers while adhering to the 3R principles, which aim to reduce, refine, and replace animal testing. This study introduces for the first time an advanced immunocompetent skin model constructed entirely from induced pluripotent stem cell (iPSC)-derived cell types, including fibroblasts (iPSC-FB), keratinocytes (iPSC-KC), and fully integrated dendritic cells (iPSC-DC). To evaluate the skin model's capacity, the model was treated topically with a range of well-characterized skin sensitizers varying in potency. The results indicate that the iPSC-derived immunocompetent skin model successfully replicates the physiological responses of human skin, offering a robust and reliable alternative to animal models for skin sensitization testing, allowing detection of extreme and even weak sensitizers. By addressing critical aspects of immune activation and cytokine signaling, this model provides an ethical, comprehensive tool for regulatory toxicology and dermatological research.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251336296"},"PeriodicalIF":6.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regeneration. 通过血浆炎症和骨再生生物标志物监测镁合金植入物的骨整合和降解。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-05-05 eCollection Date: 2025-01-01 DOI: 10.1177/20417314241290595

Eduarda Mota-Silva, Diana C Martinez, Giuseppina Basta, Serena Babboni, Serena Del Turco, Davide Fragnito, Stefano Salvadori, Claudia Kusmic, Leon Riehakainen, Daniele Panetta, Beatrice Campanella, Massimo Onor, Tomasz Plocinski, Wojciech Swieszkowski, Luca Menichetti

{"title":"Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regeneration.","authors":"Eduarda Mota-Silva, Diana C Martinez, Giuseppina Basta, Serena Babboni, Serena Del Turco, Davide Fragnito, Stefano Salvadori, Claudia Kusmic, Leon Riehakainen, Daniele Panetta, Beatrice Campanella, Massimo Onor, Tomasz Plocinski, Wojciech Swieszkowski, Luca Menichetti","doi":"10.1177/20417314241290595","DOIUrl":"https://doi.org/10.1177/20417314241290595","url":null,"abstract":"Magnesium-degradable implants have excellent mechanical and osteogenic properties for temporary orthopedic use but are underutilized due to insufficient methods to monitor implant osseointegration and tissue healing. This study evaluated the use of circulatory biomarkers to monitor the bilateral implantation of a Mg-alloy in rats' femurs. A total of 16 biomarkers were measured from plasma samples collected at multiple timepoints up to 90 days post-implantation. Mg-alloy, Ti-alloy, and Sham (noncritical bone defect) groups were followed with computed tomography, histological, and SEM-EDX analysis. The Sham group showed higher DKK1, OPG, VEGF, and KIM-1 levels than implanted groups. The Mg-alloy group had delayed bone regeneration due to gas release but demonstrated active regeneration up to 180 days and superior osseointegration. Elevated IL-10 and reduced FGF23 at day 28 correlated with accelerated implant degradation. These results underline the complex interactions between biomaterials and biological systems in orthopedic applications and show the value of circulating markers to follow-up implantation.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314241290595"},"PeriodicalIF":6.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12056334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of human salivary gland cell lines for modeling radiation-induced damage in three-dimensional spheroid cultures. 人唾液腺细胞系在三维球体培养中模拟辐射损伤的发展。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-04-30 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251326667

Sangeeth Pillai, Jose G Munguia-Lopez, Younan Liu, Jordan Gigliotti, Anthony Zeitouni, Joseph M Kinsella, Simon D Tran

{"title":"Development of human salivary gland cell lines for modeling radiation-induced damage in three-dimensional spheroid cultures.","authors":"Sangeeth Pillai, Jose G Munguia-Lopez, Younan Liu, Jordan Gigliotti, Anthony Zeitouni, Joseph M Kinsella, Simon D Tran","doi":"10.1177/20417314251326667","DOIUrl":"https://doi.org/10.1177/20417314251326667","url":null,"abstract":"No permanent cure exists for salivary gland (SG) damage and consequent xerostomia (dry mouth) in patients undergoing radiotherapy for head and neck cancers. The lack of commercially available healthy human SG-derived cell lines has hindered in vitro studies of radiation-induced glandular injury. In this study, we successfully immortalized and characterized two novel human major SG-derived cell lines. Leveraging these cell lines and hyaluronic-acid hydrogels, we bioengineered distinct multicellular SG spheroids and microtissues expressing key acinar, ductal, myoepithelial, and mesenchymal cell markers in long-term cultures. Further, using this platform, we developed a proof-of-concept radiation injury model, demonstrating spheroid disruption characterized by actin depolymerization, DNA damage, apoptosis, and loss of SG-specific markers following radiation exposure. Notably, these detrimental effects were partially mitigated with a radioprotective agent. Our findings demonstrate that the bioengineered SG spheroids provide a scalable and versatile platform with significant potential for disease modeling and drug testing, thereby accelerating the development of targeted therapies for radiation-induced xerostomia.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251326667"},"PeriodicalIF":6.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles: From large-scale production and engineering to clinical applications. 细胞外囊泡：从规模化生产、工程化到临床应用。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-04-30 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251319474

Yuxuan Wang, Jiali Xiong, Kun Ouyang, Mingwang Ling, Junyi Luo, Jiajie Sun, Qianyun Xi, Ting Chen, Yongliang Zhang

{"title":"Extracellular vesicles: From large-scale production and engineering to clinical applications.","authors":"Yuxuan Wang, Jiali Xiong, Kun Ouyang, Mingwang Ling, Junyi Luo, Jiajie Sun, Qianyun Xi, Ting Chen, Yongliang Zhang","doi":"10.1177/20417314251319474","DOIUrl":"https://doi.org/10.1177/20417314251319474","url":null,"abstract":"Extracellular vesicles (EVs) have emerged as a promising strategy for treating a wide spectrum of pathologies, as they can deliver their cargo to recipient cells and regulate the signaling pathway of these cells to modulate their fate. Despite the great potential of EVs in clinical applications, their low yield and the challenges of cargo loading remain significant obstacles, hindering their transition from experimental research to clinical practice. Therefore, promoting EV release and enhancing EV cargo-loading are promising fields with substantial research potential and broad application prospects. In this review, we summarize the clinical applications of EVs, the methods and technologies for their large-scale production, engineering, and modification, as well as the challenges that must be addressed during their development. We also discuss the future perspectives of this exciting field of research to facilitate its transformation from bench to clinical reality.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251319474"},"PeriodicalIF":6.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering growth factor gradients to drive spatiotemporal tissue patterning in organ-on-a-chip systems. 在器官芯片系统中，工程生长因子梯度驱动时空组织模式。

IF 6.7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-04-18 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251326256

Timothy Hopkins, Swati Midha, Simon Grossemy, Hazel R C Screen, Angus K T Wann, Martin M Knight

{"title":"Engineering growth factor gradients to drive spatiotemporal tissue patterning in organ-on-a-chip systems.","authors":"Timothy Hopkins, Swati Midha, Simon Grossemy, Hazel R C Screen, Angus K T Wann, Martin M Knight","doi":"10.1177/20417314251326256","DOIUrl":"https://doi.org/10.1177/20417314251326256","url":null,"abstract":"Spatial heterogeneity plays a key role in the development and function of human tissues and therefore needs to be incorporated within in vitro models to maximise physiological relevance and predictive power. Here, we developed and optimised methods to generate spatial heterogeneity of hydrogel-embedded bioactive signalling molecules within organ-on-a-chip (OOAC) systems, to drive spatiotemporal tissue patterning through controlled stem cell differentiation. As an exemplar application, we spatially patterned bone morphogenetic protein-2 (BMP-2) in both closed-channel and open-chamber OOAC formats. The resulting BMP-2 gradient in 3D heparin methacryloyl/gelatin methacryloyl, successfully drove spatially divergent differentiation of human bone marrow-derived stem cells into bone-like and cartilage-like regions, mimicking the process of endochondral ossification in the growth plate. The application of hydrogel-embedded morphogens to drive spatial tissue patterning within OOAC systems represents a significant technological advancement and has broad-ranging applicability for a diverse range of tissues and organs, and a wide variety of OOAC platforms.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251326256"},"PeriodicalIF":6.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12033634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0