Comprehensive characterization of cell and tissue responses toward high hydrostatic pressure treatment: Molecular feedback and structural integrity in bone graft processing.

IF 6.7 1区 工程技术 Q1 CELL & TISSUE ENGINEERING
Journal of Tissue Engineering Pub Date : 2025-06-28 eCollection Date: 2025-01-01 DOI:10.1177/20417314251337193
Henrike Loeffler, Jan-Oliver Sass, Lorena Muelders, Julian Bauer, Oliver Friedrich, Rainer Bader, Annett Klinder, Janine Waletzko-Hellwig
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引用次数: 0

Abstract

In the field of tissue reconstruction, the development and improvement of suitable bone grafts is of increasing importance. The implementation of bone banks enables the international distribution of suitable allografts that can be used for defect reconstruction. Currently used procedures have significant drawbacks, especially regarding biomechanical and structural properties. These can be overcome by using the technique of high hydrostatic pressure (HHP) processing. To date, little is known about the impact of HHP protocol alterations including pressure-transmitting medium or temperature regarding bone graft integrity. Data of the present study show that a low-temperature and medium-pressure treatment using isotonic sodium chloride solution as the pressure-transmitting medium generated devitalized bone tissue with preserved extracellular matrix. Specifically, efficient devitalization of human primary osteoblasts (hOBs) was found starting from 150 MPa with cell death being a complex interaction between different mechanisms. Furthermore, protein denaturation in response to HHP treatment that was predominantly observed at 600 MPa led to non-significant impairment of biomechanical properties. Effects of HHP treatment on the bone tissue did not lead to any noticeable compromise in biocompatibility. Accordingly, the presented protocol may be applied as a medical device to improve the outcome of patients undergoing bone defect reconstruction with allogenic grafts.

细胞和组织对高静水压力处理反应的综合表征:骨移植过程中的分子反馈和结构完整性。
在组织重建领域,开发和改进合适的骨移植物越来越重要。骨库的实施使国际上合适的同种异体移植物可以用于缺损重建。目前使用的手术有明显的缺点,特别是在生物力学和结构特性方面。这些都可以通过使用高静水压力(HHP)处理技术来克服。迄今为止,对HHP方案改变的影响知之甚少,包括压力传递介质或温度对骨移植完整性的影响。本研究数据表明,以等渗氯化钠溶液为传压介质的低温中压处理可生成保存细胞外基质的失活骨组织。具体来说,从150兆帕开始,发现人原代成骨细胞(hOBs)的有效失活,细胞死亡是不同机制之间复杂的相互作用。此外,在600 MPa高温高压下观察到的蛋白质变性导致生物力学性能的不显著损伤。HHP治疗对骨组织的影响没有导致任何明显的生物相容性损害。因此,本方案可作为改善同种异体骨缺损重建患者预后的医疗器械。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Tissue Engineering
Journal of Tissue Engineering Engineering-Biomedical Engineering
CiteScore
11.60
自引率
4.90%
发文量
52
审稿时长
12 weeks
期刊介绍: The Journal of Tissue Engineering (JTE) is a peer-reviewed, open-access journal dedicated to scientific research in the field of tissue engineering and its clinical applications. Our journal encompasses a wide range of interests, from the fundamental aspects of stem cells and progenitor cells, including their expansion to viable numbers, to an in-depth understanding of their differentiation processes. Join us in exploring the latest advancements in tissue engineering and its clinical translation.
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