Journal of Tissue Engineering最新文献_第3页

Genetically modified bone marrow cells halt mitral cell loss by modulating inflammation and protecting against DNA damage. 基因修饰的骨髓细胞通过调节炎症和防止DNA损伤来阻止二尖瓣细胞的损失。

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-05-16 eCollection Date: 2026-01-01 DOI: 10.1177/20417314261442787

David Díaz, Eva María Baranda-Alonso, Laura Pérez-Revuelta, Juan Felipe Zapata-Acevedo, Frédéric Torossian, Adrienne Anginot, José Ramón Alonso, Marie-Caroline Le Bousse-Kerdilès, Eduardo Weruaga

{"title":"Genetically modified bone marrow cells halt mitral cell loss by modulating inflammation and protecting against DNA damage.","authors":"David Díaz, Eva María Baranda-Alonso, Laura Pérez-Revuelta, Juan Felipe Zapata-Acevedo, Frédéric Torossian, Adrienne Anginot, José Ramón Alonso, Marie-Caroline Le Bousse-Kerdilès, Eduardo Weruaga","doi":"10.1177/20417314261442787","DOIUrl":"10.1177/20417314261442787","url":null,"abstract":"Cell therapy is a promising strategy for tackling neurodegenerative diseases. The most outstanding results with this approach usually involve neuroprotection of damaged neurons at risk of death, but only with limited success. Current therapies are often based on the idea of \"one gene, one disease, one drug\" for single targets, a concept that limits their actual effectiveness. In contrast, combining different strategies can establish an advanced cell therapy that can slow down neuronal degeneration. In this study, we took advantage of the combination of cell and gene therapy, by transplanting bone marrow stem cells genetically modified to overexpress insulin-like growth factor 1 (IGF1) into a model of selective neurodegeneration, the PCD mouse. This animal is characterized by progressive neuronal loss in the olfactory bulb and alterations in IGF1 levels, among other symptoms. Using different techniques (cell cultures, viral transduction, cell transplants, flow cytometry, qPCR, ELISA, immunohistochemistry, advanced image analysis), our findings showed that neuronal death was virtually blocked, even 130 days after cell transplantation, a result clearly more successful than previous studies. The effects of this transplant are based in part on the regulation of neuroinflammation, increasing the proportion of reactive microglia and reducing that of proinflammatory microglia. In addition, IGF1 overexpression dramatically reduced DNA damage in mutant animals via IGF binding protein 3 pathway: this enhances neuroprotection by complementing the basal effect of cell therapy itself. In summary, our work supports the idea that combining therapeutic approaches and their synergies is a more effective tactic for combating neuronal loss.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261442787"},"PeriodicalIF":10.1,"publicationDate":"2026-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13180132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147973492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibro-adipogenic progenitors enhance functional and structural properties of human 3D tissue engineered skeletal muscles. 纤维脂肪祖细胞增强人体三维组织工程骨骼肌的功能和结构特性。

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-04-25 eCollection Date: 2026-01-01 DOI: 10.1177/20417314261441552

Roy Augustinus, Lotte A de Ridder, Dongxu Zheng, Marnix Franken, Judit Balog, Patrick J van der Vliet, Alessandro Iuliano, Remko Goossens, Johanna I Hamel, W W M Pim Pijnappel, Jessica C de Greef, Silvère M van der Maarel

{"title":"Fibro-adipogenic progenitors enhance functional and structural properties of human 3D tissue engineered skeletal muscles.","authors":"Roy Augustinus, Lotte A de Ridder, Dongxu Zheng, Marnix Franken, Judit Balog, Patrick J van der Vliet, Alessandro Iuliano, Remko Goossens, Johanna I Hamel, W W M Pim Pijnappel, Jessica C de Greef, Silvère M van der Maarel","doi":"10.1177/20417314261441552","DOIUrl":"10.1177/20417314261441552","url":null,"abstract":"Human skeletal muscle models often lack important supportive cell types. Here we developed a co-culture three-dimensional tissue engineered skeletal muscle (3D-TESM) model by combining myogenic progenitors (MPs) with genetically-matched immortalized fibro-adipogenic progenitors (iFAPs). FAPs play a crucial physiological role in myogenesis, tissue remodeling and extracellular matrix (ECM) formation. We demonstrate that co-culture 3D-TESMs effectively recapitulate these processes under controlled conditions, thereby enhancing contractile force, muscle tissue integrity and longevity, as well as improving ECM deposition compared to MP-only 3D-TESMs. Moreover, using pro-fibrotic and pro-adipogenic cell culture compositions we were able to mimic pathological features typically observed in muscular dystrophies: excessive ECM production and the formation of fatty infiltrations. This study provides an advanced skeletal muscle model, with enhanced functional and structural properties, capable of recapitulating pathophysiological processes that require FAPs.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261441552"},"PeriodicalIF":10.1,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a skeletal muscle model to study extracellular vesicle dynamics. 设计一个骨骼肌模型来研究细胞外囊泡动力学。

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-04-05 eCollection Date: 2026-01-01 DOI: 10.1177/20417314261427541

María Fernández-Rhodes, Rowan Rimington, Jacob Fleming, Andrew J Capel, Owen G Davies, Mark P Lewis

{"title":"Engineering a skeletal muscle model to study extracellular vesicle dynamics.","authors":"María Fernández-Rhodes, Rowan Rimington, Jacob Fleming, Andrew J Capel, Owen G Davies, Mark P Lewis","doi":"10.1177/20417314261427541","DOIUrl":"10.1177/20417314261427541","url":null,"abstract":"Skeletal muscle (SM) functions both mechanically and as a secretory organ, releasing myokines and extracellular vesicles (EVs) involved in myogenic regulation and inter-tissue communication. While 3D bioengineered SM models are widely used for studying muscle physiology, few have been applied to investigate EV dynamics. This study optimised a 3D SM model to support mature myotube formation and evaluated its utility for SM-EV analysis. Myosin heavy chain expression was reduced at higher Matrigel® concentrations (40%-60% v/v), highlighting the importance of matrix composition in model design. EVs were successfully isolated using size-exclusion chromatography and ultrafiltration, with yield influenced by cellular differentiation status. Common EV markers (Alix, CD9, CD63) were consistently expressed. Importantly, sarcoplasmic reticulum markers α- and β-sarcoglycan were identified in SM-EV preparations. These findings validate our SM model as a defined platform for studying SM-EV biology and defining molecular cargo.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261427541"},"PeriodicalIF":10.1,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13053972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early vascularization as a key feature of muscle-in-vein grafts for peripheral nerve repair. 早期血管化是静脉内肌肉移植修复周围神经的关键特征。

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-04-03 eCollection Date: 2026-01-01 DOI: 10.1177/20417314261437253

Federica Zen, Giulio Ferrero, Alessandro Crosio, Debora Molinaro, Giulia Ronchi, Stefano Geuna, Giovanna Gambarotta, Stefania Raimondo

{"title":"Early vascularization as a key feature of muscle-in-vein grafts for peripheral nerve repair.","authors":"Federica Zen, Giulio Ferrero, Alessandro Crosio, Debora Molinaro, Giulia Ronchi, Stefano Geuna, Giovanna Gambarotta, Stefania Raimondo","doi":"10.1177/20417314261437253","DOIUrl":"10.1177/20417314261437253","url":null,"abstract":"Peripheral nerve injuries significantly impair quality of life due to limited regenerative capacity, which is affected by factors such as neuroma formation, injury severity, scarring, and comorbidities. The Muscle-in-Vein (MIV) repair technique, consisting of a vein filled with skeletal muscle fibers, has emerged as a promising alternative to nerve autografts. This approach supports regeneration by providing growth factors, guiding axonal growth, enhancing Schwann cell migration, and limiting scar and neuroma formation. However, its clinical use is currently restricted mainly to short gaps in sensory digital nerves, and the biological mechanisms underlying its effectiveness remain incompletely understood. In this study, we investigated the role of muscle fibers in the early phases of nerve regeneration, with a particular focus on vascularization. An 8 mm gap in rat median nerves was repaired using the MIV technique and analyzed at 3, 7, 14, and 21 days post-injury. Immunofluorescence analysis demonstrated complete macrophage infiltration and well-organized vascularization throughout the entire graft as early as 7 days post-injury. Consistently, RNA sequencing at early time points revealed significant enrichment of pathways associated with vascular development and identified key angiogenesis-related genes. Notably, our findings indicate partial anastomosis between vessels originating from the nerve stumps and those within the muscle component of the graft. These results suggest that the success of muscle-in-vein nerve repair strategy may be due to an early vascularization process mediated by the synergistic contribution of both muscle and vein.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261437253"},"PeriodicalIF":10.1,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13051101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D bioprinting of skin equivalents: Towards functional wound healing models. 皮肤等效物的3D生物打印：迈向功能性伤口愈合模型。

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-03-30 eCollection Date: 2026-01-01 DOI: 10.1177/20417314251407023

Adrian Perez-Barreto, Roxana Moscalu, Carlo Tremolada, Marco Domingos, Adam Reid, Jason Wong

{"title":"3D bioprinting of skin equivalents: Towards functional wound healing models.","authors":"Adrian Perez-Barreto, Roxana Moscalu, Carlo Tremolada, Marco Domingos, Adam Reid, Jason Wong","doi":"10.1177/20417314251407023","DOIUrl":"10.1177/20417314251407023","url":null,"abstract":"Developing physiologically relevant human skin models remains a critical challenge in regenerative medicine and disease modelling, particularly for chronic wounds that involve persistent inflammation and vascular dysfunction. Recent advances in 3D bioprinting provide improved spatial organisation and reproducibility compared with other engineering strategies, enabling the fabrication of skin equivalents with increasing structural and cellular complexity. Nevertheless, most current models do not capture the dynamic crosstalk between immune and vascular systems, which is central to wound healing and tissue homoeostasis. This review surveys recent progress in engineering skin models that incorporate immune and vascular elements, and discusses the biological and technological barriers that continue to limit their integration. We also highlight emerging strategies, including organoids, 4D bioprinting, and computational approaches, that may enable next-generation platforms. By more accurately modelling the wound microenvironment, such advances could accelerate translation from laboratory innovation to clinical application.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314251407023"},"PeriodicalIF":10.1,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13036355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147592810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipose mesenchymal stem cell-derived nanovesicles as a therapeutic strategy for oral mucosal regeneration after chemotherapy in a rat model. 脂肪间充质干细胞衍生的纳米囊泡作为大鼠化疗后口腔黏膜再生的治疗策略。

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-03-26 eCollection Date: 2026-01-01 DOI: 10.1177/20417314261430133

Hyeop Oh, Deogil Kim, Sun Jun Lee, Jung Eun Choi, Soo-Hong Lee, Bo Hae Kim

{"title":"Adipose mesenchymal stem cell-derived nanovesicles as a therapeutic strategy for oral mucosal regeneration after chemotherapy in a rat model.","authors":"Hyeop Oh, Deogil Kim, Sun Jun Lee, Jung Eun Choi, Soo-Hong Lee, Bo Hae Kim","doi":"10.1177/20417314261430133","DOIUrl":"10.1177/20417314261430133","url":null,"abstract":"Chemotherapy-induced oral mucositis (CIOM) is a debilitating complication with limited therapeutic options. Mesenchymal stem cells (MSCs) promote tissue repair through paracrine signaling, and stem cell-derived nanovesicles (SC-NVs) have emerged as a scalable, cell-free therapeutic alternative. However, the regenerative potential of SC-NVs has not been investigated in the context of CIOM. This study evaluated the regenerative effects of SC-NVs derived from human adipose-derived MSCs (AD MSCs) in vitro assays and a rat CIOM model. Transcriptomic profiling showed enrichment of wound healing and angiogenesis-related genes in AD MSCs. SC-NVs were produced by serial extrusion and characterized by nanoscale size and reproducible protein content. Following intralesional injection, SC-NVs localized to the ulcer bed and remained detectable for up to 5 days. SC-NV treatment enhanced epithelial regeneration, promoted angiogenesis, and reduced inflammatory markers in vitro and in vivo. These findings support SC-NVs as a scalable, cell-free therapeutic platform for CIOM.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261430133"},"PeriodicalIF":10.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13031731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-sprayable, multi-functional hydrogel for effective prevention of peritoneal adhesions. 双喷，多功能水凝胶，有效预防腹膜粘连。

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-03-24 eCollection Date: 2026-01-01 DOI: 10.1177/20417314261427227

Hyunjung Kim, Wonmoon Song, M Nivedhitha Sundaram, Changyub Lee, Xiang Li, Kyoung-Ha So, Juan M Melero-Martin, Nathaniel S Hwang

{"title":"Dual-sprayable, multi-functional hydrogel for effective prevention of peritoneal adhesions.","authors":"Hyunjung Kim, Wonmoon Song, M Nivedhitha Sundaram, Changyub Lee, Xiang Li, Kyoung-Ha So, Juan M Melero-Martin, Nathaniel S Hwang","doi":"10.1177/20417314261427227","DOIUrl":"10.1177/20417314261427227","url":null,"abstract":"Over 90% of patients experience peritoneal adhesions after abdominal surgery, necessitating the use of anti-adhesion barriers. To effectively prevent tissue adhesions, strategies that combine physical and chemical blocking are essential. A self-healing hydrogel presents physical blocking by ensuring prolonged retention at the target site. To achieve this, dynamic covalent bonds (DCBs) between polyvinyl alcohol (PVA) and amino phenylboronic acid (APBA) are introduced. In this study, PVA and chondroitin sulfate type C (CS(C)) conjugated with APBA (CSAPBA) are employed to develop a dual-sprayable, self-healing, and anti-inflammatory hydrogel barrier. The hydrogel offers both physical barrier and anti-inflammatory benefits, facilitated by ROS scavenging effect of APBA and macrophage polarization effect of CS(C). As a result, in vivo rat studies show that dual-spraying CSAPBA and PVA forms a self-healing hydrogel that effectively prevents adhesions through both physical and chemical mechanisms. These results demonstrate that the PVA/CSAPBA hydrogel is a promising candidate for preventing post-surgical adhesions.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261427227"},"PeriodicalIF":10.1,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13014009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147521394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the electrical stimulation of bone cells based on an induced transmembrane potential model and intracellular calcium levels. 基于诱导跨膜电位模型和细胞内钙水平评估骨细胞的电刺激。

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-03-23 eCollection Date: 2026-01-01 DOI: 10.1177/20417314251414697

Meike Bielfeldt, Lam Vien Che, Kai Budde-Sagert, Nils Arbeiter, Susanne Staehlke, Sascha Spors, Ursula van Rienen, Henrike Rebl, Julius Zimmermann

{"title":"Evaluating the electrical stimulation of bone cells based on an induced transmembrane potential model and intracellular calcium levels.","authors":"Meike Bielfeldt, Lam Vien Che, Kai Budde-Sagert, Nils Arbeiter, Susanne Staehlke, Sascha Spors, Ursula van Rienen, Henrike Rebl, Julius Zimmermann","doi":"10.1177/20417314251414697","DOIUrl":"10.1177/20417314251414697","url":null,"abstract":"Electrical stimulation holds promise for enhancing bone healing; however, it has not yet seen widespread clinical adoption. A significant obstacle is the limited understanding of the biological mechanisms involved and the electric field parameters required to trigger them. It has been observed that the intracellular calcium ion concentration increases upon electrical stimulation, possibly via the activation of voltage-gated calcium channels. In this work, we introduced a digital twin framework to rationally choose stimulation parameters. We aimed to induce a transmembrane potential sufficient to activate the voltage-gated calcium channels. We focused on kilohertz-frequency stimulation, which offers advantages for clinical translation, and applied electrical stimuli using a well-established direct-contact stimulation chamber. By combining this with live-cell calcium imaging, we observed the immediate stimulation effect. We found that a stimulation at <math><mrow><mn>1</mn></mrow> </math> kHz or <math><mrow><mn>100</mn></mrow> </math> kHz, adjusted to induce a transmembrane potential of about <math><mrow><mn>10</mn></mrow> </math> mV, did not alter the intracellular calcium concentration. In contrast, direct current stimulation at <math><mrow><mn>5</mn></mrow> </math> V consistently increased intracellular calcium concentrations. However, our results indicate that this effect is not caused by the electric field itself but by electrochemical by-products leading to local changes in the pH value. We further confirmed that chemical stimulation could reproduce the effect. The presented workflow enables researchers to distinguish between purely electrical and mixed electrochemical stimulation. It is easily transferable and contributes to a more precise understanding of the effects of electrical stimulation.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314251414697"},"PeriodicalIF":10.1,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13010028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety and efficacy of bio-engineered, autologous dermo-epidermal skin grafts in reconstructive surgery: 1-year results of a prospective, randomized, intra-patient controlled, multicenter phase II clinical trial. 生物工程自体真皮-表皮皮肤移植物在重建手术中的安全性和有效性：一项为期1年的前瞻性、随机、患者内对照、多中心II期临床试验结果

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-03-23 eCollection Date: 2026-01-01 DOI: 10.1177/20417314261429663

Frederique M Kemme, Anouk Pijpe, Matthea M Stoop, Paul P M van Zuijlen, Martin Meuli, Clemens Schiestl, Fabienne Hartmann-Fritsch, Bong-Sung Kim, Kathrin Neuhaus, Jan A Plock, Daniel Rittirsch, Kathi Mujynya, Ernst Reichmann, Sophie Böttcher-Haberzeth, Melinda Farkas, Jenny Bressan, Marcello Zamparelli, Ilaria Mataro, Carlo Petroccione, Alex Pontini, Bruno Azzena, Daniela Marino, Esther Middelkoop

{"title":"Safety and efficacy of bio-engineered, autologous dermo-epidermal skin grafts in reconstructive surgery: 1-year results of a prospective, randomized, intra-patient controlled, multicenter phase II clinical trial.","authors":"Frederique M Kemme, Anouk Pijpe, Matthea M Stoop, Paul P M van Zuijlen, Martin Meuli, Clemens Schiestl, Fabienne Hartmann-Fritsch, Bong-Sung Kim, Kathrin Neuhaus, Jan A Plock, Daniel Rittirsch, Kathi Mujynya, Ernst Reichmann, Sophie Böttcher-Haberzeth, Melinda Farkas, Jenny Bressan, Marcello Zamparelli, Ilaria Mataro, Carlo Petroccione, Alex Pontini, Bruno Azzena, Daniela Marino, Esther Middelkoop","doi":"10.1177/20417314261429663","DOIUrl":"10.1177/20417314261429663","url":null,"abstract":"Extensive full-thickness defects remain challenging to reconstruct, as various techniques used, such as split-thickness skin grafts, often result in donor site morbidity and functional and cosmetic scar sequelae. This study evaluated the safety and efficacy of denovoSkin™-a bio-engineered autologous dermo-epidermal skin graft consisting of patient-derived epidermal and dermal cells cultured within an extracellular matrix-compared to autologous split thickness skin grafts (STSG). Twenty-three patients (mean age 37.4 years; 65% male; 70% post-burn scars), received both denovoSkin™ and STSG on comparable wound areas. At 3 months, scar quality was significantly better for denovoSkin™ than for STSG (POSAS observer total score 23.4 vs 27.9; p = 0.008), with benefits maintained at 12 months. Elasticity parameters also consistently favored denovoSkin™, and the ratio of covered surface area to donor site was markedly higher (8.5 vs 0.9; p < 0.001). DenovoSkin™ is a safe and effective treatment for full-thickness skin defects, providing favorable long-term skin quality. Clinical Trial Registration: Clinicaltrials.gov NCT03394612.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261429663"},"PeriodicalIF":10.1,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13013987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147521353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in esophageal organoids: from construction to applications. 食管类器官的研究进展：从构建到应用。

IF 10.1 1区工程技术

Journal of Tissue Engineering Pub Date : 2026-03-18 eCollection Date: 2026-01-01 DOI: 10.1177/20417314261429669

Junling An, Wenjing Wen, Huixian Li, Ping Wang, Yinuo Liu, Gaofeng Liang

{"title":"Advances in esophageal organoids: from construction to applications.","authors":"Junling An, Wenjing Wen, Huixian Li, Ping Wang, Yinuo Liu, Gaofeng Liang","doi":"10.1177/20417314261429669","DOIUrl":"10.1177/20417314261429669","url":null,"abstract":"Esophageal organoids are self-assembled 3D microtissues derived from pluripotent or adult stem cells. They accurately recapitulate the cellular composition, tissue arrangement, and functional properties of the native esophagus. Emerging as powerful tools in disease research, they overcome key limitations of 2D cultures and animal models, such as poor microenvironmental simulation, species differences, and ethical concerns. Based on literature published in the past decades, this review summarizes recent advances in esophageal organoid construction, characterization, and applications in developmental biology, disease modeling, drug screening, and regenerative medicine. The current challenges, such as standardizing culture procedures, genetic instability, and microenvironmental complexity, are critically analyzed. We also highlight innovations such as microfluidic integration and high-throughput automation that improve model fidelity (accuracy and reliability) and support clinical translation. Future interdisciplinary efforts are expected to expand the role of esophageal organoids in precision medicine, enabling personalized diagnosis and therapy for esophageal disorders.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"17 ","pages":"20417314261429669"},"PeriodicalIF":10.1,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13009874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0