Journal of Tissue Engineering最新文献_第3页

CRISPRa genome-wide screen identifies novel gene targets for osteogenic cell engineering. CRISPRa全基因组筛选鉴定成骨细胞工程的新基因靶点。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-11-14 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251390038

Jacob D Weston, Hunter Levis, Brandon Lawrence, Rodrigo Somoza, Robby D Bowles

{"title":"CRISPRa genome-wide screen identifies novel gene targets for osteogenic cell engineering.","authors":"Jacob D Weston, Hunter Levis, Brandon Lawrence, Rodrigo Somoza, Robby D Bowles","doi":"10.1177/20417314251390038","DOIUrl":"10.1177/20417314251390038","url":null,"abstract":"Bone tissue regeneration and fracture healing remain a significant challenge for physicians, with nonunion failures occurring in an estimated 5%-10% of bone-healing treatments. The autologous bone graft has long been the gold standard of treatment. However, these procedures suffer from persistent donor-site morbidity and extended surgery times, while still having high revision and nonunion failure rates. Cell therapies and tissue engineering strategies utilizing stem cells have been considered as promising alternatives to autologous bone grafts. Here, we explore the concept of using CRISPR-activation (CRISPRa) as a cell-engineering tool to drive osteogenesis without exogenous growth factors. We present a genome-wide CRISPRa screen in adipose-derived stem cells (ASCs) to identify upregulation targets that drive osteogenesis. Top targets from the screen, SPRED2 and ATXN7L3B, demonstrated significant increases in alkaline phosphatase activity and mineralization in monolayer and 3D culture. These results are the first evidence of these genes as osteogenic targets in ASCs.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251390038"},"PeriodicalIF":7.0,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145541083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions. 在静态和自由落体动态条件下，免疫分离的胰岛嵌入胰腺细胞外基质的不同应激反应。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-10-27 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251383295

Isaura Borges-Silva, Marluce da Cunha Mantovani, Minh Danh Anh Luu, Alan Gorter, Theo Borghuis, Naschla Gasaly, Mari Cleide Sogayar, Paul deVos, Marina Trombetta-Lima

{"title":"Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditions.","authors":"Isaura Borges-Silva, Marluce da Cunha Mantovani, Minh Danh Anh Luu, Alan Gorter, Theo Borghuis, Naschla Gasaly, Mari Cleide Sogayar, Paul deVos, Marina Trombetta-Lima","doi":"10.1177/20417314251383295","DOIUrl":"10.1177/20417314251383295","url":null,"abstract":"Pancreatic islet transplantation offers great promise for the treatment of type 1 diabetes, yet the functional decline of islets after isolation remains a major obstacle. Increasing evidence highlights the endoplasmic reticulum (ER) as a critical regulator of islet cell survival under stress. We explored how ex vivo culture conditions affect encapsulated islet resilience under ER-stress. Two conditions were assessed: (i) incorporation of decellularized porcine pancreatic extracellular matrix (ECM) into alginate microcapsules, and (ii) free-fall dynamic pre-conditioning culture. Human islets were encapsulated in alginate with or without ECM, cultured under static or dynamic conditions, and exposed to acute ER-stress followed or not by a recovery period. Dynamic culture preserved viability and enhanced glucose responsiveness. ECM-containing capsules showed reduced inflammatory marker expression, while encapsulation in alginate-only capsules led to more pronounced changes associated with ECM remodeling. Under ER-stress, the dynamic culture, especially combined with ECM, maintained cell function and reduced cell death. Gene profiles indicated improved stress adaptation and ECM remodeling. These results highlight ECM enrichment and dynamic culture as good strategies to maintain islet survival and functionality.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251383295"},"PeriodicalIF":7.0,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12575931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145431589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amelioration of intervertebral disc degeneration using engineered extracellular vesicle-delivered ZDHHC5 via inhibiting PANoptosis. 通过抑制PANoptosis，利用工程细胞外囊泡递送ZDHHC5改善椎间盘退变。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-10-27 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251351011

Tong Chen, Helong Zhang, Songtao Lv, Yue Xu, Wei Ren, Yifan Wei, Tianyou Zhang, Yunru Ge, You Lv, Ruya Li, Hui Che, Kunxin Xie, Yongxin Ren, Cheng Ma

{"title":"Amelioration of intervertebral disc degeneration using engineered extracellular vesicle-delivered ZDHHC5 via inhibiting PANoptosis.","authors":"Tong Chen, Helong Zhang, Songtao Lv, Yue Xu, Wei Ren, Yifan Wei, Tianyou Zhang, Yunru Ge, You Lv, Ruya Li, Hui Che, Kunxin Xie, Yongxin Ren, Cheng Ma","doi":"10.1177/20417314251351011","DOIUrl":"10.1177/20417314251351011","url":null,"abstract":"Intervertebral disc degeneration (IDD) is a common condition and a leading cause of chronic low back pain, affecting millions of individuals worldwide. Human Umbilical Cord Mesenchymal Stromal Cell (hUCMSC)-derived extracellular vesicles (EVs) are emerging as a promising therapeutic strategy for IDD. However, the limited production yield and unclear mechanisms by which EV contents mediate their therapeutic effects have hindered the clinical application of EVs. In this study, using transcriptomic data and single-cell RNA sequencing, we identify PANoptosis as a key mechanism driving the progression of IDD. Furthermore, parathyroid hormone (PTH) enhances the secretion of hUCMSC-derived EVs and alters their cargo composition, which may contribute to their improved therapeutic effects. Mechanistically, PTH-preconditioned EVs, enriched with ZDHHC5, ameliorate PANoptosis by modulating ZBP1 transcription through competitive inhibition of YBX1 phosphorylation via palmitoylation. Our findings provide strong support for a cell-free therapeutic strategy utilizing EVs from PTH-preconditioned MSCs for IDD treatment and propose the ZDHHC5/YBX1/ZBP1 axis as a novel molecular target for inhibiting PANoptosis, thus paving the way for clinical translation and broader healthcare applications.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251351011"},"PeriodicalIF":7.0,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12575936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145431595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stage-specific cardiotoxicity induced by bisphenol A using human pluripotent stem cell-derived 2D- and 3D-cardiomyocyte models. 利用人多能干细胞衍生的2D和3d心肌细胞模型研究双酚A诱导的阶段特异性心脏毒性。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-10-23 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251383006

Soon-Jung Park, Seong Woo Choi, Yun-Gwi Park, Hye-Eun Shim, Ji-Hee Choi, Kang Moo Huh, Sung-Hwan Moon, Sun-Woong Kang

{"title":"Stage-specific cardiotoxicity induced by bisphenol A using human pluripotent stem cell-derived 2D- and 3D-cardiomyocyte models.","authors":"Soon-Jung Park, Seong Woo Choi, Yun-Gwi Park, Hye-Eun Shim, Ji-Hee Choi, Kang Moo Huh, Sung-Hwan Moon, Sun-Woong Kang","doi":"10.1177/20417314251383006","DOIUrl":"10.1177/20417314251383006","url":null,"abstract":"Bisphenol A (BPA), a widely used industrial chemical with endocrine-disrupting properties, raises developmental and cardiotoxicity concerns. We established a stage-specific cardiotoxicity platform using human pluripotent stem cell (hPSC)-derived cardiomyocytes in two-dimensional and three-dimensional (3D) cultures. BPA exposure at ⩾10 µM significantly reduced cell viability and downregulated pluripotency and cardiac lineage markers such as OCT4, NKX2-5, and cTnT in a stage-dependent manner. Electrophysiological analysis revealed that acute exposure to 10 µM BPA disrupted action potentials in hPSC-derived cardiomyocytes, inducing membrane depolarization and rhythm disturbances. Furthermore, 3D cardiac tissues treated with 10 or 50 µM BPA exhibited severe mitochondrial deformation and impaired contractile function, as observed by TEM and beating analysis. Reproducing these effects in a personalized hPSC line validated the platform's applicability for patient-specific toxicity assessment. These findings highlight the importance of integrating developmental stage-specific and 3D human-relevant models for comprehensive cardiotoxicity evaluation of environmental chemicals such as BPA.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251383006"},"PeriodicalIF":7.0,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12559640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human three-dimensional engineered muscle tissue characterization and intramyocellular lipid modeling. 人体三维工程肌肉组织表征和细胞内脂质建模。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-10-23 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251382710

Tianxin Cao, Eric Finnemore, Jon Hill, Shuo Wang, Chuanyu Wang, Heather Robinson, Jorge Villalona, Suzanne Segal, Curtis R Warren

{"title":"Human three-dimensional engineered muscle tissue characterization and intramyocellular lipid modeling.","authors":"Tianxin Cao, Eric Finnemore, Jon Hill, Shuo Wang, Chuanyu Wang, Heather Robinson, Jorge Villalona, Suzanne Segal, Curtis R Warren","doi":"10.1177/20417314251382710","DOIUrl":"10.1177/20417314251382710","url":null,"abstract":"Three-dimensional engineered muscle tissues (EMTs) are transformative tools for modeling skeletal muscle physiology and pathology in vitro. Here, we perform a comprehensive comparison of EMTs derived from primary human myoblasts (hP-Myo) and hiPS-derived myoblasts (hiPS-Myo) to evaluate their structural, functional, and transcriptional characteristics. Contractile performance was quantified using a magnetic force-sensing platform, revealing that hP-Myo EMTs generate ~2 fold higher twitch forces and enhanced tetanic responses compared to hiPS-Myo EMTs. Tissue architecture and maturation were assessed and demonstrated significant larger myofiber diameters in hP-Myo EMTs. Transcriptomic profiling highlighted that hP-Myo EMTs maintain a mature skeletal muscle-like signature, marked by enriched pathways linked to sarcomere organization and fast-/slow-twitch fiber specification. To model metabolic dysfunction, hiPS-Myo EMTs were subjected to lipid overload, recapitulating hallmarks of intracellular lipid (IMCL) accumulation, including impaired contractility, blunted force-frequency responses, and dysregulated lipid metabolism genes.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251382710"},"PeriodicalIF":7.0,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12559675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood mononuclear cells induce accelerated vascular remodeling under acute inflammation in vitro. 体外急性炎症下血单核细胞加速血管重构。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-10-16 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251381716

Raghav Soni, Manabu Shirai, Tetsuji Yamaoka, Atsushi Mahara

引用次数: 0

Angiogenesis induction using organoid-tissue modules: A platform for modular vessel construction. 使用类器官组织模块诱导血管生成：模块化血管构建平台。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-10-16 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251376104

Jin Ju Park, Eunjeong Seo, HyeRan Gwak, Jieun Lee, Hyun Ji Kim, Soyeon Jeong, Junhyung Kim, SangHyuk Lee, Jaejin Cho

{"title":"Angiogenesis induction using organoid-tissue modules: A platform for modular vessel construction.","authors":"Jin Ju Park, Eunjeong Seo, HyeRan Gwak, Jieun Lee, Hyun Ji Kim, Soyeon Jeong, Junhyung Kim, SangHyuk Lee, Jaejin Cho","doi":"10.1177/20417314251376104","DOIUrl":"10.1177/20417314251376104","url":null,"abstract":"Angiogenesis is essential for successful tissue regeneration, particularly in clinical contexts such as ischemic injury, wound healing, and reconstructive therapies. However, the establishment of functional vasculature remains a major limitation in organoid-based systems. In this study, we developed vascularized organoid tissue modules (Angio-TMs) by incorporating human umbilical vein endothelial cells (HUVECs) into scaffold-free, self-organized constructs. Remarkably, the inclusion of HUVECs at 1% of the total cell population was sufficient to generate highly reproducible and structurally stable Angio-TMs, which exhibited clear endothelial differentiation and vascular functionality both in vitro and in vivo. Furthermore, inhibition of transforming growth factor (TGF)-β signaling in Angio-TMs led to a 2.5-fold increase in vessel length density, demonstrating a substantial enhancement in angiogenic potential. These findings highlight Angio-TMs as a robust and modular platform for engineering vascularized tissues and underscore their translational relevance in regenerative medicine and tissue transplantation.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251376104"},"PeriodicalIF":7.0,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12536209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145345964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of an artificial natural killer cell mimicking system to target tumour cells. 针对肿瘤细胞的人工自然杀伤细胞模拟系统的设计。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-09-27 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251349675

Vaishali Chugh, Vijaya Krishna Kanala, Dagmar Quandt, Suainibhe Kelly, Damien King, Lasse D Jensen, Jeremy C Simpson, Abhay Pandit

{"title":"Design of an artificial natural killer cell mimicking system to target tumour cells.","authors":"Vaishali Chugh, Vijaya Krishna Kanala, Dagmar Quandt, Suainibhe Kelly, Damien King, Lasse D Jensen, Jeremy C Simpson, Abhay Pandit","doi":"10.1177/20417314251349675","DOIUrl":"10.1177/20417314251349675","url":null,"abstract":"NK cell mimics are assemblies of a cell membrane and a template that replicate biomimetic features and physicochemical properties, respectively. To develop this targeted drug delivery system, gelatin microspheres (cG) were fabricated using a water-in-oil emulsion and reinforced via DMTMM cross-linking to exhibit tunable Young's modulus, a critical parameter for cell-material interactions. These microspheres were subsequently coated with membranes derived from the human NK cell line KHYG-1 to form biomimetic NK cell mimics (cGCM), combining physicochemical control with bioinspired functionality. These engineered cGCM were non-toxic, non-inflammatory, and capable of reducing macrophage uptake by ~10% when incubated with differentiated THP-1 cells. In vitro studies demonstrated significant interaction/ proximity of the cGCM with cancer cells in 2D cultures of breast cancer cells (MDA-MB-231), 3D spheroids of liver (HepG2), and colon (HT-29) cancer cell models, and a zebrafish breast cancer xenograft (MDA-MB-231) model. The cGCM also evaded macrophage detection in a Kdrl:EGFP Spil:Ds Red zebrafish model. Furthermore, in a pilot assessment, loading and release of the sialyltransferase inhibitor (STI, 3Fax-Peracetyl Neu5Ac) using cGCM significantly reduced α-2,6 sialylation in 2D cultures of MDA-MB-231 cells, demonstrating the STI's intact functionality in inhibiting sialylation. By integrating bioinspired membranes with mechanically tunable gelatin-based carriers, our system demonstrates a multifunctional immune-mimicking platform with relevance to tissue engineering, tumour modelling, immune modulation, and drug delivery. These findings offer a promising foundation for future therapeutic strategies in cancer research and immuno-engineering.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251349675"},"PeriodicalIF":7.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12477366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hair regeneration: Mechano-activation and related therapeutic approaches. 头发再生：机械激活和相关的治疗方法。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-09-25 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251362398

Sun Young Nam, Shreyas Kumar Jain, Amal George Kurian, Ishik Jeong, Byung Cheol Park, Kiwon Ban, Jonathan C Knowles, Hae-Won Kim

{"title":"Hair regeneration: Mechano-activation and related therapeutic approaches.","authors":"Sun Young Nam, Shreyas Kumar Jain, Amal George Kurian, Ishik Jeong, Byung Cheol Park, Kiwon Ban, Jonathan C Knowles, Hae-Won Kim","doi":"10.1177/20417314251362398","DOIUrl":"10.1177/20417314251362398","url":null,"abstract":"Hair regrowth through mechano-stimulation and other therapeutic approaches has emerged as a significant area of research in regenerative medicine. This review examines recent advances in hair regeneration strategies, with a particular focus on mechanical stimulation and complementary treatments. Studies have demonstrated that skin stretching can activate hair follicle stem cells and promote hair growth under specific conditions and durations. This process involves intricate signaling interactions, particularly through the WNT and BMP pathways, and follows a two-stage mechanism that recruits and modulates the function of macrophages. Mechanical stimulation induces the release of growth factors such as HGF and IGF-1, which activate stem cells and support hair follicle regeneration. Beyond mechanical activation, emerging hair restoration therapies, including MSC transplantation, MSC secretome therapy, and platelet-rich plasma treatments, have shown promising results. These innovative strategies overcome the limitations of conventional therapies, offering effective solutions for various types of hair loss. Additionally, here we discuss the molecular mechanisms underlying hair follicle growth and repair, the influence of external factors, and novel hair follicle formation processes, such as chimeric follicle development and follicular neogenesis. Special attention is given to the roles of dermal papilla cells and their interactions with mesenchymal cells in promoting hair regrowth. The key strategies and underlying mechanisms discussed in this review will drive future research and potential clinical applications.","PeriodicalId":17384,"journal":{"name":"Journal of Tissue Engineering","volume":"16 ","pages":"20417314251362398"},"PeriodicalIF":7.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12464413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Tendon tissue engineering: An overview of biologics to promote tendon healing and repair". “肌腱组织工程：促进肌腱愈合和修复的生物制剂概述”的勘误。

IF 7 1区工程技术

Journal of Tissue Engineering Pub Date : 2025-09-25 eCollection Date: 2025-01-01 DOI: 10.1177/20417314251380714

引用次数: 0