N. Inouchi, Emiko Takei, M. Asaoka, Yoshinori Kawamura, S. Sakamoto, H. Fuwa
{"title":"Some Properties of Endosperm Starches of Foxtail Millet (Setaria italica Beauv.)","authors":"N. Inouchi, Emiko Takei, M. Asaoka, Yoshinori Kawamura, S. Sakamoto, H. Fuwa","doi":"10.5458/JAG1972.40.397","DOIUrl":"https://doi.org/10.5458/JAG1972.40.397","url":null,"abstract":"1)世界各地から収集したアワ種子の内,胚乳のヨウ素呈色反応から澱粉のアミロース含量:の比較的高いもの(試料A,B),中位のもの(試料C,D)およびもち種と想定されるもの(試料E,F)について澱粉の特性を調べた.澱粉のアミロース含量(酵素・クロマト法による画分1)は,AとBは約31%,C22%,D14%,EとFは0%で,AとBは比較的高く,CとDは中程度,EとFは想定した通りもち種であった.アミロペクチンの鎖長分布にはほとんど差がなかった.DSC糊化特性は,糊化開始(To)・ピーク(Tp)・終了(Tc)温度がそれぞれ64~69,70~77,76~84℃の範囲にあり,糊化熱(dH)は10.0~18.OJ/9の範囲であった.各澱粉粒の酵素分解はトウモロコシ澱粉粒の2~3倍程度分解が進み,他の穀物澱粉粒の場合にも見られたようにもち種の澱粉粒はうるち種のものより分解されやすい傾向であった.2)澱粉・ヨウ素複合体吸収曲線の特性値のうち最大吸収を示す波長λmaxと青価(680nmによる吸光度)との間には高い正の相関があり,また青価は酵素・クロマト法による画分1あるいは示差走査熱量測定による糊化熱との間にそれぞれ高い正および負の相関を示すことがわかった.","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"19 1","pages":"397-401"},"PeriodicalIF":0.0,"publicationDate":"1993-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90587143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Shibuya, T. Yamauchi, H. Chaen, M. Nakano, S. Sakai, M. Kurimoto
{"title":"The Formation of Amylose-Granules from Cyclomaltodextrin Glucanotransferase by a-Cyclodextrin","authors":"T. Shibuya, T. Yamauchi, H. Chaen, M. Nakano, S. Sakai, M. Kurimoto","doi":"10.5458/JAG1972.40.375","DOIUrl":"https://doi.org/10.5458/JAG1972.40.375","url":null,"abstract":"B. stearothermophzlus由来のCGTaseを高濃度のα-CDに作用させることによりアミロース粒子が生成することを見出し,その生成条件ならびにその諸性質を調べ,以下の結果を得た.1) アミロース粒子の生成は,主に反応温度,基質濃度に影響され,α-CD濃度35%,反応温度30℃で最高の生成率96%を示した.2) 他の細菌起源のCGTaseにおいてもアミロース粒子の生成が認められた.3) 得られるアミロース粒子は3~20gumの球状で,そのλmaxは560~580nmを示し,数平均分子量は5500~7000で,グルコース・ユニットとして34~43個からなるアミロースであった.4) 本物質はX線回折により澱粉のB形X線回折図を示し,偏光十字が観察されることから規則性のある結晶構造を有するアミロースであることがわかった.","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"179 1","pages":"375-381"},"PeriodicalIF":0.0,"publicationDate":"1993-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80087239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Glucosyl Lactoside (O-, β-D-Galactopyranosyl- (1→4)-O-β-D-Glucopyranosyl α-D-Glucopyranoside) Synthesized by the Intermolecular Transglucosylation Using α-Glucosidase","authors":"Keiko Sato, K. Oku, M. Yoneyama, Y. Tsujisaka","doi":"10.5458/JAG1972.40.391","DOIUrl":"https://doi.org/10.5458/JAG1972.40.391","url":null,"abstract":"The enzymatic transglucosylation of three kinds of α-glucosidase (from rat intestine, rice seed and Aspergillus) with lactose as an acceptor was studied. We found out that the three a-glucosidases were capable of synthesizing trisaccharide, which corresponds to glucosyl lactoside (O-β-D-galactopyranosyl-(1→4)-O-β-D-glucopyranosyl α-D-glucopyranoside) on gas-liquid chroma-tographic analysis, the same as the case of cyclomaltodextrin glucanotransferase (CGTase) reported. The maximum yield of the trisaccharide (3.0%) was obtained with rat intestinal a-glucosidase, and the other two a-glucosidases gave same yield (2.3%). β-Galactosidase converted the trisaccharide into the disaccharide which corresponds to neotrehalose on GLC analysis, and galactose, α-Glucosidase from rice seed also hydrolyzed the trisaccharide into lactose and glucose. These results suggest that the trisaccharide is glucosyl lactoside, and it is the common transglucosylation product of all α-glucosidases used in this study. Neotrehalose was also observed in all the reaction mixture. The α-glucosidases seem to recognize the anomeric hydroxyl group of lactose as a transglucosylation site as well as CGTase.","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"23 3 1","pages":"391-396"},"PeriodicalIF":0.0,"publicationDate":"1993-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75052247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Miyoshi, K. Kumeno, Masako Ookubo, Masae Takahashi, N. Nakahama
{"title":"X-ray Diffraction and Rheological Properties of Gels Formed from Mixtures of Waxy Corn and Corn Starches","authors":"E. Miyoshi, K. Kumeno, Masako Ookubo, Masae Takahashi, N. Nakahama","doi":"10.5458/JAG1972.40.383","DOIUrl":"https://doi.org/10.5458/JAG1972.40.383","url":null,"abstract":"モチトウモロコシおよびトウモロコシ澱粉の種々の混合割合による20%糊液を調製し,初日の保存温度の異なる2種の保存条件の下で,X線回折とレオロジー特性により比較検討し,次のような結果を得た.1)X線回折図形における結晶性の回復は,モチトウモロコシとトウモロコシ澱粉では顕著に異なり,混合澱粉は両者の中間的挙動を示した.2)貯蔵弾性率はモチトウモロコシ澱粉は小さく,トウモロコシ澱粉は非常に大きいが,混合澱粉は比較的モチトウモロコシ澱粉に近い挙動を示した.3)20%濃度である1:1混合澱粉は,10%トウモロコシ単独澱粉ゲルよりも貯蔵弾性率は低い値を示し,モチトウモロコシ澱粉を混合することはむしろ軟弱なゲルを形成して経時変化も抑制されることが示された.4)相対回折強度による各試料の結晶化過程の挙動は,その混合割合に対応して連続的に変化する傾向が認められた.5)老化過程の比較において,モチトウモロコシ澱粉では老化にともなう結晶化,弾性率の増大はほぼ同_...経過をたどると考えられ,トウモロコシ澱粉では結晶化が緩和してもゲルの硬化はさらに進行することが認められた.混合澱粉の各老化過程は,それぞれ特徴的な挙動を示した.6)老化初期における保存温度はいずれの試料においても老化過程全体に影響を及ぼすことが認められ,老化初期の状態がその老化の進行を潜在的に決定する主因となる可能性が示唆された.","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"27 1","pages":"383-389"},"PeriodicalIF":0.0,"publicationDate":"1993-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76373680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production of Pectinase and Xylanase by Aspergillus sojae and Its Effect on the Qualitative Improvement of Jute Fibre.","authors":"S. H. Talukder, C. Ara, G. Mohiuddin, S. Rahman","doi":"10.5458/JAG1972.40.361","DOIUrl":"https://doi.org/10.5458/JAG1972.40.361","url":null,"abstract":"Jute is a lignocellulosic bast fibre and contains non-fibrous materials. As a result, it is coarse, hard and stiff and requires softening. Partial removal of non-fibrous materials would make the jute cuttings considerably softened.l-3) Chemical and microbiological processes for softening of jute cuttings have been reported by several workers.4-7) The crude enzymes of Aspergillus terreus appeared to be useful for hydrolytic removal of pectin and hemicellulose from low quality jute.5' 6' Hence the present study has been carried out to produce pectinlyase [EC 4.2.2.10], polygalacturonase [EC 3.2.1.15] and xylanase [EC 3.2.1.32] from a pectinolytic fungus, Aspergillus sojae to examine their effects on the softening of low quality jute and jute cuttings.","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"50 1","pages":"361-364"},"PeriodicalIF":0.0,"publicationDate":"1993-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84265108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Hara, K. Fujita, H. Nakano, N. Kuwahara, S. Kitahata
{"title":"Digestibility of Glucosyl Lactoside (O-β-D-Galactopyranosyl- (1→4)-O-β-D-Glucopyranosyl α-D-Glucopyranoside) in Human and Its Utilization on Intestinal Flora","authors":"K. Hara, K. Fujita, H. Nakano, N. Kuwahara, S. Kitahata","doi":"10.5458/JAG1972.40.403","DOIUrl":"https://doi.org/10.5458/JAG1972.40.403","url":null,"abstract":"O-β-D-Galactopyranosyl-(1→4)-β-D-glucopyranosyl α-D-glucopyranoside(glucosyl lactoside, GL-95) was hardly hydrolyzed by human saliva, artificial gastric juice and porcine pancreas, but was hydrolyzed about 30% by rat intestinal aceton powder in vitro. GL-95 was selectively utilized by Bifidobacterium, and hardly utilized by Clostridium, Eubacterium and Escherichia.","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"207 1","pages":"403-406"},"PeriodicalIF":0.0,"publicationDate":"1993-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75705285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Moisture Sorption of Sodium Carboxymethyl Starch","authors":"K. Boki, N. Kawasaki, K. Minami, Sachie Tadokoro","doi":"10.5458/JAG1972.40.365","DOIUrl":"https://doi.org/10.5458/JAG1972.40.365","url":null,"abstract":"1)バレイショ澱粉(PS)を用いて置換度の異なるカルボキシメチル澱粉(Na-CMS)を調製し,これらの水分収着特性について明らかにした.置換度0.3以下のNa-CMSの水分含量はPSと大差はなく,置換度0.6以上のNa-CMSの水分含量はPSに比べて水分活性0.7付近で著しい立ち上がりを示した.2)収着熱のピークと置換度の結果から,水分の収着とともに膨潤による新しい収着サイトが出現するためには,残存する水酸基の数がある一定値以上必要であることが判明した.置換度の上昇に伴い,Na-CMS内部の水酸基が膨潤によって収着表面に出現しにくく,出現した収着サイトの収着能も低下することが判明した.3)PSの水分収着のエソトロピー変化は,Na-CMSよりかなり小さくなった.Na-CMS(1),(2)の水分収着のエソトロピー変化は,エネルギー的に三つに,Na-CMS(3)~(5)は二つに分けることができた.Na-CMS(3)~(5)は,カルボキシメチル基が多く導入されているため構造が不安定になり,そのため収着水の影響を受けやすく,水分収着のエソトロピー変化が低下した後,エネルギー的に安定な一定の状態をとりやすくなった.4)水分収着等温線への収着理論式の適合性を検討した結果,適合範囲が広く,理論値と実験値の平均誤差,標準偏差,標準誤差の値が小さい理論式は,Henderson式,Chung and Pfost式,Smith式であった.Chung and Pfost式とHenderson式の定数の値から,置換度が大きいNa-CMSの微細構造は,不安定で収着水の影響をより受けやすく,膨潤性は高くなることがわかった.Smith式の定数の値から,置換度が大きいNa-CMSほど,澱粉粒子と結合状態にある水分子は少なく,澱粉粒子内部に凝縮水が多く存在すると推測された.","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"51 1","pages":"365-373"},"PeriodicalIF":0.0,"publicationDate":"1993-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83186360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Factors Relating to Digestibility of Raw Starch by Amylase","authors":"T. Noda, Y. Takahata, T. Nagat","doi":"10.5458/JAG1972.40.271","DOIUrl":"https://doi.org/10.5458/JAG1972.40.271","url":null,"abstract":"The purpose of the present study was to find the variations in average granule size, amylose content, gelatinization properties (onset temperature, To ; gelatinization heat, OH) by differential scanning calorimetry (DSC) and digestibility of raw starch by crude and crystalline glucoamylases for 30 kinds of sweet potato starches . Further, the relationships among these starch properties were also studied to determine the factors relating to digestibility of raw starch by amylase. Significant negative correlations were observed between average granule size and digestibilityby each one of the two glucoamylases. ΔH was also negatively correlated with digestibility by each one of the two glucoamylases . No significant correlations existed between two other properties (amylose content and To) and digestibility by each one of the two glucoamylases.","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"33 6 1","pages":"271-276"},"PeriodicalIF":0.0,"publicationDate":"1993-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79384739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Conversion of Sucrose into Laminaribiose Using Sucrose Phosphorylase, Xylose Isomerase and Laminaribiose Phosphorylase","authors":"M. Kitaoka, Takashi Sasaki, H. Taniguchi","doi":"10.5458/JAG1972.40.311","DOIUrl":"https://doi.org/10.5458/JAG1972.40.311","url":null,"abstract":"Laminaribiose was synthesized from sucrose by coupling reactions of sucrose phosphorylase, xylose isomerase and laminaribiose phosphorylase in the presence of a catalytic amount of Pi. For the synthesis, a reaction mixture (2ml) containing 200 mM sucrose, 20 mM Pi, 0.47 U/ml sucrose phosphorylase, 0.072 U/ml xylose isomerase, and 0.37 U/ml Laminaribiose phosphorylase in 50 mM imidazole-HCl buffer (pH 7.0) was used. The concentration of Laminaribiose reached 110 mM after 48 hr incubation at 37°C. Laminaritriose was also formed in the same mixture after 15 hr, and its concentration reached 22 mM.","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"84 1","pages":"311-314"},"PeriodicalIF":0.0,"publicationDate":"1993-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76728463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Ikushima, Tadayoshi Sekiya, Shigeki Oku, Ikuo Yashiki, Nobuhiro Kuwabara, K. Hara, H. Hashimoto, I. Okura
{"title":"Development of a Durable Deodorizer Film.","authors":"K. Ikushima, Tadayoshi Sekiya, Shigeki Oku, Ikuo Yashiki, Nobuhiro Kuwabara, K. Hara, H. Hashimoto, I. Okura","doi":"10.5458/JAG1972.40.315","DOIUrl":"https://doi.org/10.5458/JAG1972.40.315","url":null,"abstract":"","PeriodicalId":17372,"journal":{"name":"Journal of the Japanese Society of Starch Science","volume":"39 1","pages":"315-317"},"PeriodicalIF":0.0,"publicationDate":"1993-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75633872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}