Conversion of Sucrose into Laminaribiose Using Sucrose Phosphorylase, Xylose Isomerase and Laminaribiose Phosphorylase

Laminaribiose was synthesized from sucrose by coupling reactions of sucrose phosphorylase, xylose isomerase and laminaribiose phosphorylase in the presence of a catalytic amount of Pi. For the synthesis, a reaction mixture (2ml) containing 200 mM sucrose, 20 mM Pi, 0.47 U/ml sucrose phosphorylase, 0.072 U/ml xylose isomerase, and 0.37 U/ml Laminaribiose phosphorylase in 50 mM imidazole-HCl buffer (pH 7.0) was used. The concentration of Laminaribiose reached 110 mM after 48 hr incubation at 37°C. Laminaritriose was also formed in the same mixture after 15 hr, and its concentration reached 22 mM.