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Long-term efficacy of diatomaceous earth, SilicoSec® against three major stored grain insect pests
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2025-01-04 DOI: 10.1016/j.jspr.2024.102531
Haleh Mortazavi , Ahmet Güray Ferizli , Umut Toprak , Şule Tütüncü , Mevlut Emekci , Nesrin Ormanoğlu
{"title":"Long-term efficacy of diatomaceous earth, SilicoSec® against three major stored grain insect pests","authors":"Haleh Mortazavi ,&nbsp;Ahmet Güray Ferizli ,&nbsp;Umut Toprak ,&nbsp;Şule Tütüncü ,&nbsp;Mevlut Emekci ,&nbsp;Nesrin Ormanoğlu","doi":"10.1016/j.jspr.2024.102531","DOIUrl":"10.1016/j.jspr.2024.102531","url":null,"abstract":"<div><div>Diatomaceous earth (DE), which possesses low mammalian toxicity and stability on grain, has promising potential in controlling stored grain pests. However, only a few published studies focus on the effectiveness during the long term storage. The current study assessed the long-term efficacy of DE, SilicoSec® on <em>Sitophilus granarius</em>, <em>Rhyzopertha dominica</em>, and <em>Tribolium confusum</em> using infestation with two adults per kg of wheat. The population density of each pest was monitored throughout one year in 50 kg of stored wheat treated with three doses of SilicoSec® (500, 1000, and 2000 mg/kg grain) in drums under laboratory condition. The number of adults (dead or alive) for <em>S. granarius</em>, <em>R. dominica</em>, and <em>T. confusum</em> was 8.00 ± 1.73, 2.00 ± 0.58 and 1.00 ± 0.58, respectively, at 2000 mg/kg dose of DE by 12 months of storage. The suppression rate was 95.2, 99.4, and 97.9% for <em>S. granarius</em>, <em>R. dominica</em>, and <em>T. confusum</em>, respectively, at 1000 mg/kg DE after 12 months of storage.</div><div>This study showed that wheat treated with SilicoSec® at 1000 mg/kg could be satisfactorily protected against major stored grain pests for a year. SilicoSec® has great residual efficacy for a year, which is generally not achievable with residual grain protectants.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102531"},"PeriodicalIF":2.7,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insecticidal efficacy of two diatomaceous earths from Serbia against the Plodia interpunctella (Lepidoptera: Pyralidae) infestation on maize
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2025-01-03 DOI: 10.1016/j.jspr.2024.102538
Dragana Z. Predojević , Filip N. Vukajlović , Vesna M. Perišić , Ana Mitrovski Bogdanović , Snežana B. Pešić
{"title":"Insecticidal efficacy of two diatomaceous earths from Serbia against the Plodia interpunctella (Lepidoptera: Pyralidae) infestation on maize","authors":"Dragana Z. Predojević ,&nbsp;Filip N. Vukajlović ,&nbsp;Vesna M. Perišić ,&nbsp;Ana Mitrovski Bogdanović ,&nbsp;Snežana B. Pešić","doi":"10.1016/j.jspr.2024.102538","DOIUrl":"10.1016/j.jspr.2024.102538","url":null,"abstract":"<div><div>The evaluation of diatomaceous earths (DEs) against a wide range of major stored-product insect pests highlights their possible importance in integrated pest management protocols, as an environmentally friendly alternative to conventional insecticides. This study was conducted to evaluate the insecticidal efficacy of two DEs from Serbia (DE S-1 and DE S-2, mined in the Kolubara region) and one commercial formulation (Protect-It®) applied at rates of 0.5, 1.0 and 1.5 g/kg, as maize protectants against the infestation of <em>Plodia interpunctella</em>. The total number of <em>P. interpunctella</em> adults that survived the treatment with DEs was recorded and the life history traits (mean developmental duration (MDD), sex ratio, adult longevity and fecundity) were investigated. Although Protect-It® proved 100% effective in protecting maize from <em>P. interpunctella</em> infestation in all three applied rates, both DEs from Serbia also showed significant efficacy. The results indicate that DE S-1 and DE S-2 could effectively suppress <em>P. interpunctella</em> infestation on maize, based on significantly low number of emerged adults, prolonged MDD and shorter adult longevity.</div><div>Fecundity was lower in the treatments with DE since only one pair of moths successfully mated and laid 41 eggs. No losses in maize weight were registered in treatment with Protect-It®. In DE S-1 and S-2 treatments, maize weight losses were significantly lower in comparison to the negative control. Diatomaceous earth S-1 applied at a rate of 1.5 g/kg and DE S-2 applied at rates 1.0 and 1.5 g/kg significantly influenced mean developmental duration and longevity of <em>P. interpunctella</em> adults and showed excellent insecticidal potential in this moth control and suppression on maize. The results of this study provide the necessary information about the possibility of preventive application of Serbian DEs, against <em>P. interpunctella</em> infestation.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102538"},"PeriodicalIF":2.7,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insecticidal potential of diatomaceous earth against Callosobruchus maculatus (Coleoptera: Chrysomelidae) infesting stored cowpea, Bambara groundnut and soybean in the Sudano-Guinean climatic conditions of Cameroon
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2025-01-02 DOI: 10.1016/j.jspr.2024.102533
Daniel Kosini , Moïse Adamou , George Tchindebe , Jean W. Goudoungou , Tagne G. Fotso , Mohammadou Moukhtar , Elias N. Nukenine
{"title":"Insecticidal potential of diatomaceous earth against Callosobruchus maculatus (Coleoptera: Chrysomelidae) infesting stored cowpea, Bambara groundnut and soybean in the Sudano-Guinean climatic conditions of Cameroon","authors":"Daniel Kosini ,&nbsp;Moïse Adamou ,&nbsp;George Tchindebe ,&nbsp;Jean W. Goudoungou ,&nbsp;Tagne G. Fotso ,&nbsp;Mohammadou Moukhtar ,&nbsp;Elias N. Nukenine","doi":"10.1016/j.jspr.2024.102533","DOIUrl":"10.1016/j.jspr.2024.102533","url":null,"abstract":"<div><div>Diatomaceous earth (DE), derived from fossilized diatoms, serves as an effective alternative to synthetic insecticides for grain protection. This study assessed two DE products, Silico-Sec® and Fossil Shield®, against <em>Callosobruchus maculatus</em> in cowpea, Bambara groundnut, and soybean stored in Cameroon's Sudano-Guinean agro-ecological zone. Four DE doses (0.5, 1, 1.5, and 2 g/kg grain) were evaluated, along with malathion at 0.5 g/kg. Mortality was recorded over six days, and oviposition and F1 progeny production were measured at day seven and at F1 emergence, respectively. Both DE formulations exhibited dose-dependent mortality, with Silico-Sec® achieving 96.5% mortality in cowpea within three days (LD<sub>50</sub> = 0.54 g/kg) and Fossil Shield® reaching it in four days (LD<sub>50</sub> = 0.33 g/kg) at 2 g/kg. High efficacy was similarly noted for soybean and Bambara groundnut, with both DEs surpassing 95% mortality within 3 day at the highest dose. Furthermore, Silico-Sec® and malathion significantly reduced oviposition on cowpea by 98%, while Fossil Shield® achieved a 71% reduction; analogous trends were observed for F1 progeny inhibition. Silico-Sec® demonstrated efficacy comparable to malathion across all parameters, underscoring its potential as a sustainable option in integrated pest management against <em>C. maculatus</em> in this region of Cameroon.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102533"},"PeriodicalIF":2.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing insecticide residues in stored maize in Southern and Central Benin
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2024-12-31 DOI: 10.1016/j.jspr.2024.102529
Olivier Tonato , Elie Ayitondji Dannon , Saturnin Hounsou , Daniel C. Chougourou , Manuele Tamò
{"title":"Assessing insecticide residues in stored maize in Southern and Central Benin","authors":"Olivier Tonato ,&nbsp;Elie Ayitondji Dannon ,&nbsp;Saturnin Hounsou ,&nbsp;Daniel C. Chougourou ,&nbsp;Manuele Tamò","doi":"10.1016/j.jspr.2024.102529","DOIUrl":"10.1016/j.jspr.2024.102529","url":null,"abstract":"<div><div>In Benin, protection of maize against storage insect pests remains a major challenge and motivates maize growers to rely on synthetic chemicals. The overuse/misuse of such compounds can lead to high residue levels and potential health risks. This current study was designed to assess insecticide residues in stored maize in Southern and Central Benin. Thirty-five (35) samples of shelled, husked and dehusked maize were collected in both areas especially in seven major maize production municipalities namely Adjohoun, Aplahoue Djidja Kétou, Lokossa, Ouesse and Toffo. Samples were taken from different types of storage structures in five villages (one sample per village) in each municipality. Both villages and growers were selected randomly. Chemical residues were extracted using the QuEChERS method with 4 replicates while analysis was performed using High Performance Liquid Chromatography (HPLC) coupled with an Ultra-Violet spectrophotometer technique for the four commonly applied chemicals on stored maize in the study area. Residues of Pirimiphos-methyl, Aluminium phosphide and Permethrin were found in 80–100, 40–100, and 0–100% of the samples collected, respectively, but none contained Lambda-Cyhalothrin. In total, 97% of the samples contained at least one of the pesticides tested, and 94% contained at least one of the pesticides tested at a concentration above the European Union international standard (Maximum residual limits) (MRL). Of these, 77%, 91% and 14% of the samples contained residues above the MRL for aluminum phosphide, pirimiphos-methyl and permethrin, respectively. Data were discussed with regard to potential human health concerns.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102529"},"PeriodicalIF":2.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Postharvest practices and aflatoxin content of cowpea (Vigna unguiculata L. Walp.) grain in Burkina Faso
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2024-12-31 DOI: 10.1016/j.jspr.2024.102528
Amidou S. Ouili , Mahamadi Nikiema , Cheik Omar Tidiane Compaoré , Zakheleni Palane Dube , Mikaila Kinore , Ynoussa Maiga , Inoussa Ilboudo , Aboubakar Sidiki Ouattara
{"title":"Postharvest practices and aflatoxin content of cowpea (Vigna unguiculata L. Walp.) grain in Burkina Faso","authors":"Amidou S. Ouili ,&nbsp;Mahamadi Nikiema ,&nbsp;Cheik Omar Tidiane Compaoré ,&nbsp;Zakheleni Palane Dube ,&nbsp;Mikaila Kinore ,&nbsp;Ynoussa Maiga ,&nbsp;Inoussa Ilboudo ,&nbsp;Aboubakar Sidiki Ouattara","doi":"10.1016/j.jspr.2024.102528","DOIUrl":"10.1016/j.jspr.2024.102528","url":null,"abstract":"<div><div>Cowpea is one of the most important crops in addressing food insecurity in less developed rural communities. However, due to certain inappropriate production and storage conditions, this crop is likely to be contaminated by fungi capable of producing mycotoxins. Hence, the objective of this study was to determine on post-harvest processing for cowpea grains in the three climatic zones, Sahelian, Sudano-Sahelian and Sudanian, of Burkina Faso and to assess the aflatoxin B<sub>1</sub>, B<sub>2</sub>, G<sub>1</sub> and G<sub>2</sub> content of these grains. A questionnaire was administered to 69 producers to determine the post-harvest processing methods of cowpea grain. The questionnaire was supported with direct observations. Post-harvest handling of cowpea grains by producers, predominantly female (74.4%), revealed that pod drying was on plastic sheets (39.13%), plastic bags (13.04%), terraces (36.23%), on the ground (4.35%), on the roof of sheds (4.35%) or on the roof of houses (2.9%). Whereas the main materials used for grain storage were sealed cans (49.28%) and Purdue Improved Crop Storage (PICS) bags (26.09%). Insects (36.23%) and fungi (10.14%) were the main biotic constraints reported. The aflatoxin contents of cowpea grain samples collected were relatively low (0.02 μg kg<sup>−1</sup> to 2.56 μg kg<sup>−1</sup>) compared to the limit values set by the codex (0.5–15 μg kg<sup>−1</sup>) for foodstuffs. The study provides valuable insights into mycotoxin risks, offering guidance for regulatory measures. Its findings are applicable to other regions of Sub-Saharan Africa and developing areas, presenting practical solutions to enhance post-harvest management and address contamination challenges.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102528"},"PeriodicalIF":2.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temperature mediates life history, energy reserves, and host selection of Trogoderma granarium Everts (Coleoptera: Dermestidae)
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2024-12-28 DOI: 10.1016/j.jspr.2024.102516
Azita Dehghan , Mahsa Safa , Ehsan Borzoui
{"title":"Temperature mediates life history, energy reserves, and host selection of Trogoderma granarium Everts (Coleoptera: Dermestidae)","authors":"Azita Dehghan ,&nbsp;Mahsa Safa ,&nbsp;Ehsan Borzoui","doi":"10.1016/j.jspr.2024.102516","DOIUrl":"10.1016/j.jspr.2024.102516","url":null,"abstract":"<div><div>We examined how diet and temperature affect developmental time and survival of immature stages, adults’ longevity, fecundity, and energy reserves of <em>Trogoderma granarium</em> Everts (Coleoptera: Dermestidae), a cosmopolitan pest of grains and other stored products. Also, we studied whether and to what extent the temperature impacts the host selection by larvae. Beetles were reared under five temperature regimes (21, 25, 29, 33, and 37 °C, 65 ± 5% R.H. and a photoperiod of 14L: 10D h) and fed with three commodities (barley, walnut, and wheat). At 29, 33, and 37 °C, the shorter immature developmental time and higher survival rate of <em>T. granarium</em> were on wheat, followed by barley and walnut. A diapause-like state was induced in the last instar larvae fed barley at 25 and 21 °C and wheat at 21 °C. In contrast, the beetles fed walnut completed their life cycle and successfully oviposited at these temperatures. At 21 and 25 °C, walnut attracted a higher density of larvae in a free-choice condition. Conversely, wheat attracted more larvae at 29, 33, and 37 °C, and the lowest number of larvae was observed on walnut. The walnut-fed larvae had a higher content of lipids than wheat- and barley-fed larvae. The present study highlights the need to account for temperature effects when studying the food preferences of insects and to consider further characteristics that may impact host selection. Our findings allow us to anticipate the population dynamics of <em>T. granarium</em> on different commodities according to environmental temperature.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102516"},"PeriodicalIF":2.7,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low-density Pyemotes zhonghuajia effectively controls the eggs and early instar larvae of Megabruchidius dorsalis
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2024-12-27 DOI: 10.1016/j.jspr.2024.102527
Silin Chen , Siyu Chen , Jianfeng Liu , Fangling Xu , Chengxu Wu , Maofa Yang
{"title":"Low-density Pyemotes zhonghuajia effectively controls the eggs and early instar larvae of Megabruchidius dorsalis","authors":"Silin Chen ,&nbsp;Siyu Chen ,&nbsp;Jianfeng Liu ,&nbsp;Fangling Xu ,&nbsp;Chengxu Wu ,&nbsp;Maofa Yang","doi":"10.1016/j.jspr.2024.102527","DOIUrl":"10.1016/j.jspr.2024.102527","url":null,"abstract":"<div><div><em>Megabruchidius dorsalis</em> (Coleoptera: Bruchinae) is an oligophagous storage pest that damages the seeds of <em>Gleditsia</em> L. in Asia and Europe. <em>Pyemotes zhonghuajia</em> Yu, Zhang &amp; He (Acari: Pyemotidae), an endemic natural enemy of wood-boring pests, is characterized by its preference for dark environments and strong search capabilities. This study investigated the efficiency of <em>P. zhonghuajia</em> to parasitize <em>M. dorsalis</em> based on three parameters as follows: mortality rate, parasitic preference, and functional response. We found that <em>P. zhonghuajia</em> exhibited high mortality rates against the eggs, early instar larvae, and pupae of <em>M. dorsalis</em>, particularly achieving 100% mortality for eggs and 1st–2nd instar larvae within 6 h of treatment. <em>P. zhonghuajia</em> showed a greater inclination to parasitize 3rd–4th instar larvae and pupae of <em>M. dorsalis</em>. The parasitic behavior of <em>P. zhonghuajia</em> toward <em>M. dorsalis</em> eggs and 1st instar larvae conformed to a type III functional response, indicating that parasitism rate increased with host density and then decreased at higher host densities. Increased density of <em>P. zhonghuajia</em> negatively impacted its searching efficiency. In conclusion, <em>P. zhonghuajia</em> was highly efficient in parasitizing various developmental stages of <em>M. dorsalis</em> (especially eggs and early larvae) and could be used for biological control.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102527"},"PeriodicalIF":2.7,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Olfactory cues in the host-location of the cosmopolitan ecto-parasitoid Pyemotes tritici (Acarina: Pyemotidae)
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2024-12-27 DOI: 10.1016/j.jspr.2024.102525
Paolo Masini , Manuela Rebora , Valerio Saitta , Silvana Piersanti , Gianandrea Salerno
{"title":"Olfactory cues in the host-location of the cosmopolitan ecto-parasitoid Pyemotes tritici (Acarina: Pyemotidae)","authors":"Paolo Masini ,&nbsp;Manuela Rebora ,&nbsp;Valerio Saitta ,&nbsp;Silvana Piersanti ,&nbsp;Gianandrea Salerno","doi":"10.1016/j.jspr.2024.102525","DOIUrl":"10.1016/j.jspr.2024.102525","url":null,"abstract":"<div><div>Chemical ecology regarding the ectoparasitoid mite of the family <em>Pyemotidae</em> remains largely underexplored. This field warrants further attention, to enhance the effectiveness of pyemotid mite species as biological control agents against xylophagous insects and stored-product pests, and to clarify the role of these mites in the onset of itchy dermatitis in humans. This is the first investigation regarding the host-location process of <em>Pyemotes tritici</em> (LaGrèze-Fossat and Montane, 1851), an ectoparasitoid that attacks a wide range of hosts, including xylophagous insects and stored product pests. Bioassays in still air olfactometer and open arena have been performed. Females of <em>P. tritici</em>, consistent with the typical behavior of generalist parasitoids, demonstrated the ability to detect and respond to various volatile chemicals emitted from the host habitat (seasoned wood sawdust from pine and beech) and host frass of two longhorn beetles, <em>Hylotrupes bajulus</em> (L.) and <em>Trichoferus holosericeus</em> (Rossi), as well as from feces and silk of the rice moth <em>Corcyra cephalonica</em> (Stainton), while they did not respond to volatiles emitted from the larvae of the same species. Additionally, they detected non-volatile chemicals from the cuticle of <em>T. holosericeus</em>. These results highlight the importance of chemical cues, both direct and indirect, from the host in the host-location process of <em>P. tritici</em>. Moreover, we provided a useful protocol for safely rearing these potentially harmful mites. Chemical analysis of host and habitat VOCs and host larval CHCs involved in the host location process for identification of active compounds will complete the behavioural results here presented.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102525"},"PeriodicalIF":2.7,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of DNA damage in larvae and adults of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) exposed to hypoxia at different temperatures using the comet assay
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2024-12-26 DOI: 10.1016/j.jspr.2024.102524
Mohamed Y. Hashem , Shimaa S.H. Khalil , Rasha A. Zinhoum , Amira Afify , Sayeda S. Ahmed
{"title":"Detection of DNA damage in larvae and adults of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) exposed to hypoxia at different temperatures using the comet assay","authors":"Mohamed Y. Hashem ,&nbsp;Shimaa S.H. Khalil ,&nbsp;Rasha A. Zinhoum ,&nbsp;Amira Afify ,&nbsp;Sayeda S. Ahmed","doi":"10.1016/j.jspr.2024.102524","DOIUrl":"10.1016/j.jspr.2024.102524","url":null,"abstract":"<div><div>The red flour beetle, <em>Tribolium castaneum</em> is considered one of the most important pests of grains and stored products. Its adults and larvae feed on flour, grain germ and endosperm, and contaminate grains and stored products with their body parts and feces. In heavy infestation, the products become unusable for human consumption. So this study aimed to evaluate the effect of hypoxia (using non-toxic gases) on flour beetle larvae and adults under temperatures of 15, 25 and 35 °C using 97 and 98% nitrogen at several exposure periods. Also, DNA damage was measured in untreated and treated insects with LT<sub>50</sub> of 98% N<sub>2</sub> at different temperatures by comet assay. The results showed that, at 97 and 98% N<sub>2</sub>, the complete adult mortality was achieved after 17 &amp; 13 days at 15 °C, which decreased to 9 &amp; 8 days at 25 °C and 7 &amp; 6 days at 35 °C. While the larvae treated with nitrogen at 15, 25 and 35 °C needed exposure periods of 13, 9 and 4 days at 97% N<sub>2</sub> and 12, 7 and 3 days at 98% N<sub>2</sub> to achieve complete mortality. The comet assay showed that, DNA in adults and larvae was damaged at different levels according to temperatures; the tail length increased from 5.95 to 8.82 μm and DNA in tail was 10.52% increased to 12.86% when temperature increased from 15 °C to 35 °C. The tail length of treated larvae was 6.41μm increased to 7.66 μm and the DNA in tail was 9.51% increased to 11.59% when temperature increased from 25 to 35 °C. In conclusion, the results showed significant damage to the DNA of larvae and adults treated with the highest concentration and temperature used, so we recommend treating adults and larvae of <em>T. castaneum</em> with 98% N<sub>2</sub> for 6 days at 35 °C to control this pest.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102524"},"PeriodicalIF":2.7,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Control potential of Theocolax elegans (Westwood) (Hymenoptera: Pteromalidae) against Lasioderma serricorne (Fabricius) (Coleoptera: Ptinidae)
IF 2.7 2区 农林科学
Journal of Stored Products Research Pub Date : 2024-12-24 DOI: 10.1016/j.jspr.2024.102526
Qingjiao Yuan, Jianhua Lü, Linshan Du, Yafei Guo
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