Exploring the efficacy of four essential oils as potential insecticides against two stored-product insects, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae): Experimental and in silico approaches

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research Pub Date : 2025-05-14 DOI:10.1016/j.jspr.2025.102690

Imane Naimi , Hafida Bouamama , Jihane Touhtouh , Khalil Hammani , Touria Ba M'hamed

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引用次数: 0

Abstract

Rhyzopertha dominica (Fabricius, 1792) (Coleoptera: Bostrichidae) and Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae) are two major insect pests whose damage affects the quality, quantity, and commercial value of stored products. In the present study, the effectiveness of essential oils (EOs) extracted by the hydrodistillation method from Artemisia absinthium aerial parts, Melia azedarach fruits, Trigonella foenum-graecum, and Peganum harmala seeds as insecticidal agents was evaluated by examining their chemical composition, contact toxicity, and effect on the structure of the insect cuticle, as well as their in silico interactions with acetylcholinesterase and cytochrome P₄₅₀. The GC-MS results revealed that the major compounds of the investigated EOs were epicubenol (28.78 %), palmitic acid (15.07 %), dihydroactinolide (10.84 %), and thymol (9.56 %) for T. foenum-graecum EO; camphor (36.22 %) and α-thujone (30.28 %) for A. absinthium EO; capronic acid (57.85 %) and ç-Caprolactone (9.44 %) for M. azedarach EO; α-pinene (29.38 %), (9E),12(Z) linoleic acid (14.00 %) for P. harmala EO. A. absinthium EO caused high contact toxicity against R. dominica (LC₅₀ = 3.37 μL/mL, LC₉₀ = 6.23 μL/mL) and T. castaneum (LC₅₀ = 5.15 μL/mL, LC₉₀ = 8.52 μL/mL) after 96 h of exposure. These results were confirmed by the micrographs obtained by scanning electron microscopy (SEM), revealing significant cuticular lesions in both insects treated with A. absinthium EO. Additionally, the in silico molecular approach showed that epi-cubenol and dihydroactinolide (the main compounds of T. foenum-graecum EO) have a binding affinity to the AChE and cytochrome P₄₅₀ enzymes.

The EOs can create an agricultural biopesticide formulation with contact toxicity against R. dominica and T. castaneum.

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四种精油对两种储存品昆虫——灰蛾蛾（鞘翅目：拟甲科）和拟甲蛾（拟甲科）的药效研究：实验和计算机方法

灰蛾（Fabricius, 1792）（鞘翅目：虫虻科）和castaneum (Herbst, 1797)（鞘翅目：拟甲科）是影响储藏产品质量、数量和商业价值的两种主要害虫。本研究通过化学成分、接触毒性、对昆虫表皮结构的影响以及与乙酰胆碱酯酶和细胞色素P450的硅相互作用，评价了加氢蒸馏法提取的苦艾、苦楝果、三角Trigonella foenum-graecum和苦楝种子精油的杀虫效果。气相色谱-质谱分析结果表明，该药材的主要成分为表苯二酚（28.78%）、棕榈酸（15.07%）、二氢放线素（10.84%）和百里香酚（9.56%）；苦艾草EO中樟脑（36.22%）和α-图琼（30.28%）；苦楝籽提取物中含有己酸（57.85%）和 -己内酯（9.44%）；α-蒎烯（29.38%），（9E），12(Z)亚油酸（14.00%）。苦艾草EO暴露96 h后，对白僵菌（LC50 = 3.37 μL/mL, LC90 = 6.23 μL/mL）和蓖麻菌（LC50 = 5.15 μL/mL, LC90 = 8.52 μL/mL）均有较高的接触毒性。通过扫描电镜（SEM）获得的显微照片证实了这些结果，显示了苦艾草EO处理的两种昆虫的表皮明显病变。此外，硅分子方法表明，表三苯酚和二氢放线素内酯（T. foenum-graecum EO的主要化合物）与AChE和细胞色素P450酶具有结合亲和力。EOs可以创建一种具有接触毒性的农业生物农药配方，以对抗多米尼加和castaneum。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Stored Products Research 生物-昆虫学

CiteScore

5.70

自引率

18.50%

发文量

112

审稿时长

45 days

期刊介绍： The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.