Huayu Fu , Guosheng Xin , Yanshen Zhao , Cong Wang , Zhongzhi Han
{"title":"Efficient sweet potato quality detection using pruned attention-based deep learning","authors":"Huayu Fu , Guosheng Xin , Yanshen Zhao , Cong Wang , Zhongzhi Han","doi":"10.1016/j.jspr.2025.102749","DOIUrl":"10.1016/j.jspr.2025.102749","url":null,"abstract":"<div><div>Sweet potatoes are rich in essential nutrients crucial for maintaining human health, and their market value is primarily determined by external quality. To address the classification of sweet potato appearance quality, we propose a rapid assessment method for evaluating external quality. Using a dataset of 1833 images of the <em>Yanshu 25</em> variety, we adopt the MobileNetV4 series network with a reduced Universal Inverted Bottleneck (UIB) module as the core architecture. The network is trained and pruned by integrating a learning rate scheduler, a multi-head self-attention (MHSA) mechanism, and a pruning algorithm based on gated mechanisms. After pruning, the optimal model achieves an accuracy of 93.08 % across five categories, with an average detection speed of only 0.0022 s per sample. This method offers outstanding detection accuracy, faster processing speed, and significantly reduced model complexity, making it highly effective for sweet potato quality assessment.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102749"},"PeriodicalIF":2.7,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Monitoring of Tribolium castaneum (Coleoptera: Tenebrionidae) adults following exposure to abamectin by traps with 4,8-dimethyldecanal and commercial kairomone","authors":"W.M.S.S. Senevirathne , P.A.P.I. Premathilaka , W.C.P. Egodawatta , W.R. Morrison III , L.K.W. Wijayaratne","doi":"10.1016/j.jspr.2025.102742","DOIUrl":"10.1016/j.jspr.2025.102742","url":null,"abstract":"<div><div>The aggregation pheromone 4,8-dimethyldecanal (4,8-DMD) and commercially-available kairomones are used for trapping <em>Tribolium castaneum</em>, a serious stored-product pest insect but low trapping is often reported. Previous studies reported that exposure to certain chemicals enhanced their trapping but the influence of the insecticide abamectin is not known. This research aimed to determine the effect of exposing <em>T. castaneum</em> to abamectin on subsequent orientation to traps containing its aggregation pheromone 4,8-dimethydecanal and a kairomone. Under laboratory conditions, trapping with pheromone was similar up to 7.5 ppm, then trapping was reduced at 10, 15 and 20 ppm. The highest attraction (53%) to pheromone + kairomone vial observed at 2.5 ppm which was greater than adults not exposed to abamectin. Overall, trapping decreased with increasing concentrations of abamectin beyond 2.5 ppm. Under simulated warehouse conditions, the highest trapping (29%) occurred in insects exposed to 1 ppm abamectin and was not significantly different from 0 ppm (no pheromone) and 2.5 ppm. The lowest trapping (8%) occurred at 15 ppm which was not significantly different than 20 ppm. With pheromone + kairomone, the highest trapping (48.75%) obtained in adults exposed to 0 ppm abamectin which was not significantly different than 2.5 ppm exposure while the lowest recapture was 11% at 20 ppm. The combination of pheromone and kairomone in traps captured more insects than pheromone alone. But, exposure to high concentrations of abamectin reduced trapping at 14 d following exposure. Therefore, future research needs to schedule the pre-treatment with abamectin so that it will not jeopardize monitoring efforts through pheromone and/or kairomone traps to ensure protection of stored food from insect damage.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102742"},"PeriodicalIF":2.7,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of AFB1-degrading strain Bacillus subtilis HQ3 and its biodegradation mechanism","authors":"Zhengfu Feng , Wenxin Dong , Leilei Ge , Tianjiao Zhu , Wei Zhu","doi":"10.1016/j.jspr.2025.102746","DOIUrl":"10.1016/j.jspr.2025.102746","url":null,"abstract":"<div><div>Aflatoxin B1 (AFB1) is one of the most hazardous mycotoxins, and occurrences in food, feed, and their raw materials, presenting risks to the health of humans, animals and environment. Thus, study on the biodegradation of AFB1 is imperative to tackle this problem. In this study, by means of sequence analysis and phylogenetic tree construction of <em>16S rDNA</em>, five strains (HQs) with the ability to degrade AFB1 were identified. These strains showed more than 99 % identity with <em>Bacillus subtilis</em>. When cultivated in LB medium, the HQs strains were able to degrade 72.13 %–81.34 % of AFB1 at 50 μg L<sup>−1</sup>. Notably, strain HQ3 could effectively degrade 86.58 % of AFB1 at 50 μg L<sup>−1</sup> in LB medium after 4 days incubation at 35 °C, pH 9.0, and a C/N ratio of 3/2. Under optimal conditions, it could degrade 88.32 % of AFB1 at 50 μg kg<sup>−1</sup> in peanut meal.</div><div>Further analyses of the genetic function showed that strain HQ3 harbored genes encoding AFB1-degrading enzymes, such as multi-copper polyphenol oxidoreductase laccase (HQ3-Laccase) and myo-inosose-2 dehydratase (HQ3-MID). The enzymes in the cell-free supernatant of HQ3 played a major role in AFB1 degradation, with a degradation of 80.52 %. Moreover, the sequence of HQ3-Laccase exhibited 99.64 % similarity to that of CotA laccase from <em>B. amyloliquefaciens</em>, and HQ3-MID showed 99.49 % similarity to that of BADE from <em>B. subtilis</em>. The expression of the two enzyme-encoding genes increased as the AFB1 content increased from 0 to 20 or 40 μg L<sup>−1</sup>. These findings demonstrated that AFB1 could be removed by HQ3-laccase and HQ3-MID.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102746"},"PeriodicalIF":2.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hannah E. Quellhorst , Maria K. Sakka , Georgia V. Baliota , Christos G. Athanassiou , Kun Yan Zhu , William R. Morrison III
{"title":"Efficacy of amorphous silica dust for use against Sitophilus granarius and Rhyzopertha dominica as a grain protectant","authors":"Hannah E. Quellhorst , Maria K. Sakka , Georgia V. Baliota , Christos G. Athanassiou , Kun Yan Zhu , William R. Morrison III","doi":"10.1016/j.jspr.2025.102747","DOIUrl":"10.1016/j.jspr.2025.102747","url":null,"abstract":"<div><div>With the rise of insecticide resistance in common stored product insect species and fewer options for control due to increased regulatory oversight, it is imperative to explore other options for management. One option is the use of inert dusts such as diatomaceous earth (DE) or amorphous silica dust. In this study, we investigated the utility of an amorphous silica dust originally formulated for use against bed bugs, but applied here as a grain protectant for two stored products insects (<em>Sitophilus granarius</em> and <em>Rhyzopertha doninica</em>). A total of 200 g of wheat was conditioned with five concentrations of silica dust (ChinCheX Bed Bug Insecticide, 99 % amorphous silica), including: 0 (control), 100, 200, 500, and 1000 ppm. Insects were exposed continuously with condition evaluations at 7 and 14 days. Adults were then removed and the grain was kept for 65 d to assess progeny production. Mortality was 90 and 100 % for the 500 and 1000 ppm treatments for <em>R. dominica</em> and 100 % for both 500 and 1000 ppm treatments for <em>S. granarius</em>. Progeny production was less than 2 insects at 200 ppm and zero at 500 and 1000 ppm for <em>R. dominica</em>, while for <em>S. granarius</em>, there was less than 1 insect at 500 ppm and zero at 1000 ppm. Overall, we found that silica dust is an effective grain protectant on wheat against these bulk storage pests and provides a valuable option for use in an integrated pest management program.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102747"},"PeriodicalIF":2.7,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yongfu Gao, ShiBo Wang, Dingrong Xue, Liyuan Xia, Yi Wu
{"title":"Dose-dependent lethality of sulfuryl fluoride and transcriptomic insights into tolerance evolution on Cheyletus malaccensis (Oudemans) (Acari: Cheyletidae)","authors":"Yongfu Gao, ShiBo Wang, Dingrong Xue, Liyuan Xia, Yi Wu","doi":"10.1016/j.jspr.2025.102739","DOIUrl":"10.1016/j.jspr.2025.102739","url":null,"abstract":"<div><div>The use of the predatory mite <em>Cheyletus malaccensis</em> (Oudemans) for controlling stored grain pests represents an ecologically sustainable biocontrol strategy, while sulfuryl fluoride fumigation serves as an effective chemical control method. This study investigates the feasibility of combining <em>C. malaccensis</em> with sulfuryl fluoride fumigation to manage stored grain pests. Additionally, we employ transcriptomic analysis to elucidate the physiological and metabolic mechanisms that <em>C. malaccensis</em> engages under stress conditions. Under the same fumigation duration, the mortality rates of both adult and egg increased with higher concentrations of sulfuryl fluoride. The LC<sub>50</sub> for adult mites after 48 h of fumigation was approximately 1.58 g/m<sup>3</sup>, while the LC<sub>50</sub> for egg was around 26.48 g/m<sup>3</sup>. When the fumigation time for egg was extended to 60 h, the mortality rate at a concentration of 25 g/m<sup>3</sup> increased by approximately 1.4 times; after 72 h, the mortality rate at 15 g/m<sup>3</sup> rose by 3.6 times. Egg exhibited greater tolerance to sulfuryl fluoride fumigation compared to adult. Neither adult nor egg could survive in high-concentration, long-duration fumigation environments. <em>C. malaccensis</em> can be released after the fumigation has dissipated (with sulfuryl fluoride concentrations not exceeding 2.3 g/m<sup>3</sup>), allowing it to effectively establish populations in real storage facilities for long-term control. In the transcriptome data, a total of 4730 differentially expressed genes were identified, including 3489 upregulated and 1241 downregulated genes. Through GO and KEGG analysis, it was found that the pathways involved in lipid metabolism and energy metabolism processes, pathogen infection and immune response were significant.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102739"},"PeriodicalIF":2.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Current treatments and emerging approaches in stored-product insect pest management","authors":"Shimaa Ghareeb , Nehal Shaarawy , Raul Narciso C. Guedes , Youssef Dewer","doi":"10.1016/j.jspr.2025.102745","DOIUrl":"10.1016/j.jspr.2025.102745","url":null,"abstract":"<div><div>The management of stored-product insect pests is at a critical juncture, with advancements in technology and growing environmental concerns driving the development of innovative solutions. Traditional chemical-based methods, while effective, pose risks to human health and the environment, necessitating the exploration of safer, sustainable alternatives. Emerging tools such as nanotechnology offer transformative approaches, including nanopesticides and controlled-release formulations, which enhance efficacy while minimizing chemical residues. Likewise, molecular technologies like RNA interference (RNAi) and CRISPR/Cas9 enable precise pest control strategies that align with eco-friendly goals. Digital innovations, including artificial intelligence (AI) and machine learning (ML), are revolutionizing pest detection and decision-making, ensuring more accurate and timely interventions. However, further developing and implementing these technologies at scale requires transdisciplinary collaboration addressing challenges related to safety and environmental impact, as well as their regulation, and also economic feasibility. Compounding these complexities is the influence of climate change, which alters pest dynamics, geographical distributions, and the effectiveness of current management strategies. Future approaches to stored-product pest management must integrate cutting-edge technologies with adaptive, climate-resilient strategies. Emphasis on environmentally sound practices will be vital to preserving global food security and protecting stored commodities from pest damage. By balancing technological innovation with sustainability, pest management can achieve a new paradigm that is both effective and ecologically responsible.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102745"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weiwei Sun , Fu Qi , Jun Yuan , Yong Cao , Lei Wang , Lijian Zhou , Yuqing Wu , Jun Feng
{"title":"Structural behavior of stiffened steel silos subject to eccentric discharge pressures","authors":"Weiwei Sun , Fu Qi , Jun Yuan , Yong Cao , Lei Wang , Lijian Zhou , Yuqing Wu , Jun Feng","doi":"10.1016/j.jspr.2025.102741","DOIUrl":"10.1016/j.jspr.2025.102741","url":null,"abstract":"<div><div>Thin-walled cylindrical steel silos are extensively used in many industries and agricultural sectors for storing materials. Eccentric discharge is widely recognized as the most severe load case for steel silos. Accordingly, this paper aims to present the results of a finite element analysis of the buckling strength of stiffened cylindrical silos during eccentric discharge under different pressure distribution proposals. The distribution patterns of Eurocode may lead to the lowest buckling loads. Subsequently, an extensive parametric analysis is carried out to verify the influence of various factors on the buckling behavior of the stiffened silo under eccentric discharge. Ultimately, based on the buckling stress design method of Eurocode, combined with parametric and regression analyses, the simple formula for calculating the stiffened steel silo buckling resistance is proposed.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102741"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weiwei Liu , Jianhua Lü , Chunqi Bai , Yafei Guo , Jiaqin Xi , Chao Guo
{"title":"Transcriptome responses of Anisopteromalus calandrae (Hymenoptera: Pteromalidae) females to sublethal deltamethrin exposure","authors":"Weiwei Liu , Jianhua Lü , Chunqi Bai , Yafei Guo , Jiaqin Xi , Chao Guo","doi":"10.1016/j.jspr.2025.102744","DOIUrl":"10.1016/j.jspr.2025.102744","url":null,"abstract":"<div><div><em>Anisopteromalus calandrae</em> (Howard) (Hymenoptera: Pteromalidae) is a dominant parasitoid of stored grain pests. Deltamethrin is widely used for controlling stored grain pests. However, deltamethrin also has a certain potential negative impact on parasitoids due to its broad-spectrum insecticidal property, which poses a challenge for the scientific and efficient collaborative application of biological and chemical control. Thus, the toxicity of deltamethrin on female <em>A. calandrae</em> was investigated when exposed to 0.00, 0.02, 0.04, and 0.08 μg/cm<sup>2</sup> for 4 d (the corresponding female <em>A. calandrae</em> samples were referred as DM0, DM0.02, DM0.04, and DM0.08, respectively) and transcriptomic analysis was conducted to reveal their patterns of response. Compared to control (11 d), the total lethal times of <em>A. calandrae</em> females exposed to 0.02, 0.04, 0.06, 0.08, and 0.10 μg/cm<sup>2</sup> of deltamethrin was 8, 7, 7, 5, and 4 d, respectively. Transcriptomic data revealed 3040, 5377, and 4517 differentially expressed genes (DEGs) in DM0.02, DM0.04, and DM0.08 groups, compared with the control (DM0), respectively. GO and KEGG analyses indicated that pathways involved in longevity regulation, cuticle biosynthesis, fatty acid metabolism, and detoxification (cytochrome P450) were affected. WGCNA revealed a highly correlated module (turquoise) containing key resistance-related genes, such as TRINITY_DN882_c0_g1_i21. The current results are in favor of improving the compatibility of biological and chemical pest control strategies in grain storage systems.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102744"},"PeriodicalIF":2.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniel Camilo Ripoll-Aristizábal , Oscar Javier Patiño-Ladino , Juliet Angélica Prieto-Rodríguez
{"title":"Essential oils and phenylpropanoids from Piper: Bioactivity and enzyme inhibition in Sitophilus zeamais and Tribolium castaneum","authors":"Daniel Camilo Ripoll-Aristizábal , Oscar Javier Patiño-Ladino , Juliet Angélica Prieto-Rodríguez","doi":"10.1016/j.jspr.2025.102714","DOIUrl":"10.1016/j.jspr.2025.102714","url":null,"abstract":"<div><div>This study aimed to evaluate the insecticidal potential of essential oils (EOs) from four <em>Piper</em> species and selected phenylpropanoids against <em>Triboliu</em></div><div><em>m castaneum</em> and <em>Sitophilus zeamais</em>, focusing on fumigant, topical toxicity, repellent effects, and enzyme inhibition related to detoxification and motor function. EOs from <em>Piper aduncum</em>, <em>P. asperiusculum</em>, <em>P. auritum</em>, and <em>P. holtonii</em> were obtained by steam distillation and analyzed by GC-MS. Apiol, dillapiole, and myristicin were isolated from the EOs using flash chromatography, while safrole was acquired commercially. Fumigant activity was tested using the vial-in-vial method, topical toxicity via direct application, and repellency through the area preference method. Enzymatic assays for acetylcholinesterase (AChE), catalase (CAT), and glutathione S-transferase (GST) were performed spectrophotometrically. Major constituents included dillapiole and apiol in <em>P. aduncum</em>, myristicin and dillapiole in <em>P. asperiusculum</em>, safrole in <em>P. auritum</em>, and apiol and dillapiole in <em>P. holtonii</em>. <em>P. auritum</em> EO showed potent fumigant and topical toxicity against <em>T. castaneum</em> (LC<sub>50</sub>: 4.7–7.2 μL/L air; LD<sub>50</sub>: 84.4–120.0 μg/insect), while <em>P. asperiusculum</em> EO was effective topically against <em>S. zeamais</em> (LD<sub>50</sub>: 108.2–123.2 μg/insect). <em>P. holtonii</em> EO was highly repellent (90 % and 80 % repellency at 0.1 μL/cm<sup>2</sup>). Safrole exhibited the highest toxicity among constituents (LD<sub>50</sub>: 34.4 and 12.6 μg/insect for <em>S. zeamais</em> and <em>T. castaneum</em>, respectively). Structure–activity analysis indicated decreased toxicity with more methoxy groups. Enzyme inhibition ranged from low to moderate (0–50 %), suggesting secondary involvement in insecticidal action. These results support the potential of <em>Piper</em> EOs and phenylpropanoids as eco-friendly pest control agents.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102714"},"PeriodicalIF":2.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ebtesam Farah Badran , Eizz Elden A. Elshazly , Tarek Gamal Abedelmaksoud , Gehad M. Khattab , Sayeda Sayed Ahmed
{"title":"Impact of red flour beetle Tribolium castaneum (Herbst) infestation in wheat flour on benzoquinone levels and biscuit quality","authors":"Ebtesam Farah Badran , Eizz Elden A. Elshazly , Tarek Gamal Abedelmaksoud , Gehad M. Khattab , Sayeda Sayed Ahmed","doi":"10.1016/j.jspr.2025.102743","DOIUrl":"10.1016/j.jspr.2025.102743","url":null,"abstract":"<div><div>The red flour beetle, <em>Tribolium castaneum</em> (Coleoptera: Tenebrionidae), negatively impacts the quality of wheat flour and its derived products. This study examines the effects of <em>T. castaneum</em> infestation at levels of 10, 20, and 40 pairs/kg over different storage periods (4, 6, and 8 months) on biscuits produced by infested flour. Key parameters assessed include moisture content, weight, diameter, thickness, spread ratio, sensory attributes, chemical composition, and benzoquinone accumulation. Results indicate that biscuit moisture content and weight increased with both infestation level and storage duration. After 8 months, the highest infestation level (40 pairs/kg) resulted in a moisture content of 10.75 % and a weight of 14.40 g, significantly higher than the control. Diameter increased while thickness decreased, leading to an increase in the spread ratio from 3.89 to 4.63. Sensory evaluation revealed a decline in appearance, taste, and aroma, negatively impacting overall biscuit quality. Chemical composition analysis showed an increasing trend in crude protein, crude lipid, and ash, while carbohydrate and fiber were decreased, and this attributed to insect infestation level and biscuit formulation. Additionally, <em>Tribolium castaneum</em> infestation raises benzoquinone (BQ) levels in wheat flour and its biscuits, with higher insect density increasing BQ secretion, which peaks between 4 and 8 months of storage. Statistical analysis confirmed significant differences across all tested parameters. These findings highlight the progressive deterioration in biscuit quality and safety due to <em>T. castaneum</em> infestation, emphasizing the need for preventive strategies to minimize economic losses and maintain product integrity.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102743"},"PeriodicalIF":2.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}