Journal of Stored Products Research最新文献

{"title":"Machine vision for visible mold detection in stored oil-bearing raw materials under simulated conveying conditions","authors":"Xilong Tao ,&nbsp;Lijun Cao ,&nbsp;Wenqiang Zhang ,&nbsp;Baoran Hou ,&nbsp;Zicheng Jiao","doi":"10.1016/j.jspr.2026.103098","DOIUrl":"10.1016/j.jspr.2026.103098","url":null,"abstract":"<div><div>Post-storage oil-bearing raw materials may develop visible mold-related deterioration before processing or sorting, which can reduce raw material utilization, affect processing suitability, and increase the difficulty of front-end quality management. To support rapid non-destructive screening, this study developed a machine vision method for appearance-based visible mold detection in stored peanuts, corn kernels, and soybeans under simulated conveying-related conditions. Images of normal-appearing and visibly moldy samples formed under natural storage conditions were collected, and a six-class object detection dataset, OOSD-6, was constructed. To account for common imaging disturbances during conveying and screening, illumination variation, background replacement, scene recomposition, and motion blur augmentation were introduced. Based on YOLO11, an improved detection model, METW-YOLO, was developed for multi-class visible mold detection. On the validation set, METW-YOLO achieved Precision, Recall, [email protected], and [email protected]:0.95 values of 95.36%, 95.82%, 98.05%, and 95.90%, respectively, corresponding to improvements of 2.13%, 2.53%, 1.66%, and 1.82% over the baseline YOLO11. The results indicate that METW-YOLO improves detection performance under the constructed dataset and simulated conveying-related disturbance conditions. The proposed method provides a potential visual pre-screening approach for identifying visibly moldy samples before processing or sorting, and may support front-end quality management of post-storage oil-bearing raw materials.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"118 ","pages":"Article 103098"},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148179455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of postharvest heat treatment impact on insecticidal efficacy and quality parameters in commercial dried fruits","authors":"Marina Gourgouta ,&nbsp;Velisaria-Eleni Gerogianni ,&nbsp;Maria K. Sakka ,&nbsp;George Boskou ,&nbsp;Antonia Chiou ,&nbsp;Vaios T. Karathanos ,&nbsp;Michael Karvelas ,&nbsp;Christos G. Athanassiou","doi":"10.1016/j.jspr.2026.103063","DOIUrl":"10.1016/j.jspr.2026.103063","url":null,"abstract":"<div><div>This study evaluates the efficacy of postharvest heat treatment as a sustainable alternative to chemical fumigation for disinfestation of commercial dried fruits, while simultaneously assessing its impact on product quality. Corinthian currants, Medjool dates, and Turkish dried figs were artificially infested with <em>Oryzaephilus surinamensis</em> (L.)(Coleoptera: Silvanidae) and <em>Plodia interpunctella</em> (Hübner)(Lepidoptera: Pyralidae) and exposed to temperatures of 35 °C, 45 °C, and 55 °C for 2, 6, and 24 h. Complete adult mortality and reproductive suppression for both species was achieved at 55 °C in all commodities. Commodity type significantly influenced mortality, likely due to differences in fruit structure and moisture content affecting heat transfer. Sensory evaluation revealed that exposures up to 45 °C did not significantly alter appearance, aroma, or texture, while treatments at 55 °C for prolonged durations caused minor declines, particularly in figs. Analysis of bioactive microconstituents showed that moderate heating (35–45 °C) enhanced total flavonoid content and, in some cases, total phenolic content, whereas exposure at 55 °C occasionally led to lower values compared to those obtained under moderate heating. LC–MS profiling confirmed temperature-dependent increases in specific phenolic acids (e.g., vanillic, <em>p</em>-hydroxybenzoic) and flavonols (e.g., quercetin, kaempferol), while HPLC-DAD-UV analysis revealed that 5-hydroxymethylfurfural remained at trace or non-detectable levels across all treatments. This key finding serves as a robust indicator of the absence of detrimental sugar degradation or advanced Maillard reactions, proving that the proposed thermal conditions effectively preserve the dried fruit chemical integrity. Overall, the findings demonstrate that carefully optimized heat treatments can ensure complete adult mortality and progeny production suppression and preserve the nutritional and sensory quality of dried fruits, supporting their implementation as an effective, residue-free, and environmentally compatible postharvest disinfestation method.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"118 ","pages":"Article 103063"},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148179402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing postharvest quality and storability of bananas using UV-blocking and antioxidant bionanocomposite coatings enriched with nettle seed essential oil nanoemulsion","authors":"Dilara Konuk Takma ,&nbsp;Huriye Gözde Ceylan","doi":"10.1016/j.jspr.2026.103055","DOIUrl":"10.1016/j.jspr.2026.103055","url":null,"abstract":"<div><div>Biodegradable active packaging systems are emerging as sustainable alternatives to conventional plastics for extending food shelf life. Essential oil-based nanoemulsions are effective active components for the development of functional films and coatings. In this study, a novel biopolymer-based nanocomposite film was developed by incorporating nettle seed essential oil nanoemulsions (NEs) into pea protein and fenugreek seed mucilage matrix to enhance UV-blocking, antioxidant, and food preservation properties. NEs prepared with 2–4% nettle essential oil exhibited high stability (-28.90 to -37.07 mV) and nanoscale particle size (146–189 nm). Increasing NE concentration (0–20%, NF0–NF20) significantly influenced film properties, enhancing matrix interactions and structural complexity as confirmed by SEM and FTIR analyses. NE incorporation increased film thickness, while density, water vapor permeability, and solubility remained within a stable range. Oxygen permeability increased at NF10 and NF15, while NF0 and NF20 showed similar and lower values. All films showed strong UV-blocking ability with reduced visible light transmission. Mechanical properties were modulated, with decreased tensile strength (2.30–1.76 MPa) and increased elongation at break (up to 91%), indicating enhanced flexibility. In addition, total phenolic content and antioxidant capacity increased with higher NE concentration, demonstrating improved bioactive functionality. Pea protein and fenugreek seed mucilage-based nanocomposite coatings effectively delayed banana softening and maintained firmness, with NF20 showing improved control of peel color changes. Overall, the NE-incorporated films demonstrated potential for active and sustainable food packaging applications by integrating UV protection, antioxidant activity, and improved functional performance.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"117 ","pages":"Article 103055"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constantinos Th. Buchelos","authors":"Christos Athanassiou,&nbsp;Pasquale Trematerra,&nbsp;Cornel Adler","doi":"10.1016/j.jspr.2025.102922","DOIUrl":"10.1016/j.jspr.2025.102922","url":null,"abstract":"","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"117 ","pages":"Article 102922"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147945384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transgenerational effects of short-term low-temperature stress on development, fecundity, and cold tolerance of Megabruchidius dorsalis","authors":"Min Li ,&nbsp;Yonglan Pan ,&nbsp;Jingjing Shi ,&nbsp;Jun Liao ,&nbsp;Yuting Yan ,&nbsp;Silin Chen ,&nbsp;Fei Su ,&nbsp;Runa Zhao ,&nbsp;Yue Wang ,&nbsp;Lei Gao ,&nbsp;Guy Smagghe ,&nbsp;Chengxu Wu ,&nbsp;Maofa Yang","doi":"10.1016/j.jspr.2026.103053","DOIUrl":"10.1016/j.jspr.2026.103053","url":null,"abstract":"<div><div><em>Megabruchidius dorsalis</em> (Fåhraeus) (Coleoptera: Chrysomelidae) is a key field and storage pest of <em>Gleditsia sinensis</em> seeds. This study investigated the transgenerational effects of short-term low-temperature stress (5 °C and 10 °C for 1, 3, and 5 days) on the development, fecundity, and cold tolerance of <em>M. dorsalis</em>. The results showed that low-temperature stress significantly prolonged the pre-oviposition of the parental (P) generation and extended adult longevity, indicating a survival-fecundity trade-off strategy. In the F₁ generation, exposure to 10 °C for 1, 3, or 5 days notably extended the egg period, delayed reproductive timing and reduced total fecundity, revealing transgenerational effects. Adult longevity of F₁ generation was significantly influenced by temperature, stress duration, and their interaction, with distinct patterns between mated and unmated individuals and between sexes. The supercooling point (SCP; temperature at which spontaneous nucleation and ice lattice growth begin), increased with prolonged exposure at 5 °C. However, SCP and freezing point (FP; temperature at which insect fluids freeze) decreased under 10 °C treatment with exposure durations from 3 to 5 days. But, neither treatment significantly affected the SCP and FP of F₁ adults. These findings provide novel insights into the life-history adaptations and short-term cold-tolerance strategies of <em>M. dorsalis</em>, highlighting the importance of transgenerational responses for forecasting population dynamics under fluctuating temperature conditions and informing temperature-based pest management strategies.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"117 ","pages":"Article 103053"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterobacter hormaechei S27 is a potential biocontrol agent against Camellia oleifera seed mildew","authors":"Shanni Lin ,&nbsp;Xiaojun Deng ,&nbsp;Shiping Xu ,&nbsp;Xiyu Su ,&nbsp;Chunqian Leng ,&nbsp;He Li ,&nbsp;Richard C. Hamelin ,&nbsp;Guoying Zhou ,&nbsp;Yuanhao He","doi":"10.1016/j.jspr.2026.103054","DOIUrl":"10.1016/j.jspr.2026.103054","url":null,"abstract":"<div><div>Seed mildew caused by <em>Penicillium crustosum</em> seriously compromises the postharvest quality of <em>Camellia oleifera</em> seeds, leading to seed deterioration and reduced oil quality. In this study, <em>Enterobacter hormaechei</em> S27, was isolated from the rhizosphere soil of <em>C. oleifera</em>. and showed broad-spectrum antagonistic activity. The strain exhibited strong in <em>vitro</em> antifungal activity producing by a mean inhibition zone of 19.10 ± 0.3 mm and 94.90% inhibition of spore germination. Optimized fermentation conditions resulted in a 28.2-fold increase in the viable cell count of S27. The optimal wettable powder formulation consisted of bentonite, sodium lignosulfonate, and cyclodextrin, yielding a viable cell count of 6.33 × 10⁷ CFU/g and a survival rate of 52.20% after six months of storage. Within 6 d after wounded inoculation, the mildew area on <em>C. oleifera</em> seeds treated with S27 was reduced by 68.63% compared with the control. Under simulated storage conditions, the disease incidence of seeds treated with the S27 wettable powder was 17.65 ± 1.53% at 30 days, representing a 61.45% reduction compared with the control. These results suggest that <em>E. hormaechei</em> S27 is a promising candidate for the biological control of postharvest seed mildew in <em>C. oleifera</em>, although systematic biosafety evaluation is required before practical application.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"117 ","pages":"Article 103054"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entomofauna, mycotoxin accumulation and storage loss dynamics in cereals in the Tshopo province, Democratic Republic of Congo","authors":"Jeremy Berdy ,&nbsp;Hervé Ambakina ,&nbsp;Papy N'Sevolo ,&nbsp;Manon Genva ,&nbsp;Rudy Caparros Megido ,&nbsp;Frédéric Francis ,&nbsp;Marie-Laure Fauconnier","doi":"10.1016/j.jspr.2026.103049","DOIUrl":"10.1016/j.jspr.2026.103049","url":null,"abstract":"<div><div>Post-harvest losses of cereals remain a major challenge for food security in tropical regions, yet data from Central Africa are scarce. This study investigated the entomofauna, mycotoxin contamination, and storage loss dynamics of rice (<em>Oryza sativa</em> L.) and maize (<em>Zea mays</em> L.) stored under traditional conditions in the Tshopo province, Democratic Republic of Congo. Cereals were sampled at nine storage sites across three zones at 0, 30, and 60 days of storage. Insect pests were identified using morphological and molecular approaches, mycotoxins were quantified using immunochromatographic assays validated by UHPLC–MS/MS, and grain quality losses were assessed through weight, moisture, integrity, and germination measurements.</div><div>Stored cereals were dominated by three insect pests: <em>Sitophilus zeamais</em>, <em>Sitotroga cerealella</em>, and <em>Tribolium castaneum</em>, accounting for more than 98% of all collected insects. <em>S. zeamais</em> was ubiquitous and reached high densities in both crops, while <em>S. cerealella</em> showed site-specific infestations, particularly in rice. Aflatoxin contamination was detected in both crops and increased significantly during maize storage, frequently exceeding the European regulatory limit after 60 days in some zones. Fumonisins were detected exclusively in maize at low but consistent concentrations. Grain moisture readings point to a high risk of pre-storage mycotoxin accumulation, with additional in-storage aflatoxin production occurring specifically in stores with the highest moisture levels. Grain quality deteriorated during storage, with significant losses in dry weight, integrity, and germination capacity, particularly in sites with high insect pressure and mycotoxin levels. Correlation analyses revealed strong associations between insect abundance, mycotoxin accumulation, grain moisture content, and grain deterioration. Findings demonstrate that post-harvest losses in stored rice and maize in Tshopo province are driven by synergistic interactions between storage pests and fungal contamination. The study provides the first comprehensive baseline data for the region and highlights the urgent need for improved storage management to reduce food safety risks and cereal losses.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"117 ","pages":"Article 103049"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drying kinetics, moisture diffusivity, and quality preservation of mint leaves using an IoT-assisted hybrid solar dryer with air recirculation","authors":"El-Sayed Gomaa Khater ,&nbsp;Adel Hamed Bahnasawy ,&nbsp;M. Alhumedi ,&nbsp;Atef Fathy Ahmed ,&nbsp;Ali Majrashi ,&nbsp;Bassem N. Samra ,&nbsp;Ramadan ElGamal ,&nbsp;Abdallah Elshawadfy Elwakeel ,&nbsp;Amged El-Harairy ,&nbsp;Mohamed Hamdy Eid ,&nbsp;Aml Abubakr Tantawy ,&nbsp;Khaled A. Metwally","doi":"10.1016/j.jspr.2026.103059","DOIUrl":"10.1016/j.jspr.2026.103059","url":null,"abstract":"<div><div>Drying of aromatic herbs such as mint (<em>Mentha spicata L</em>.) is often constrained by unstable environmental conditions, leading to extended drying durations, inefficient energy utilization, and deterioration of product quality. In this study, an advanced IoT-enabled hybrid solar dryer integrating a flat-plate solar collector, LPG-assisted auxiliary heating, and a controlled air recirculation system was developed and experimentally evaluated to provide controlled thermal conditions and enhanced energy efficiency. Drying experiments were conducted at air temperatures of 50, 55, and 60 °C combined with recirculation ratios of 70%, 80%, and 90% under both winter and summer climatic conditions. The system utilized real-time sensing and automated control to dynamically regulate airflow distribution, maintain temperature within ±2 °C, and stabilize relative humidity inside the drying chamber. The drying performance was quantitatively evaluated using moisture ratio, drying rate, effective moisture diffusivity (D_eff), activation energy, and thermodynamic parameters including enthalpy (ΔH), entropy (ΔS), and Gibbs free energy (ΔG). The results demonstrated that increasing both drying temperature and recirculation ratio significantly enhanced moisture removal and reduced drying time due to improved heat and mass transfer conditions. Under optimal conditions (60 °C and 80% recirculation), the drying time was reduced to approximately 105–120 min, compared with 180–210 min at lower operating conditions, representing a reduction of nearly 40–50%. The final moisture content decreased from an initial range of 77–87% (wet basis) to 7.5–7.6%, meeting safe storage requirements. The effective moisture diffusivity ranged from 2.7 × 10⁻⁹ to 6.08 × 10⁻⁹ m²/s, increasing proportionally with temperature and airflow recirculation, indicating enhanced internal moisture diffusion. Seasonal analysis revealed faster drying under summer conditions; however, the hybrid configuration maintained stable performance across varying ambient conditions.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"117 ","pages":"Article 103059"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the effects of cold plasma on enhancing the stabilization of oat flour: Insights from storage characteristics, lipidomics, and flavor analysis","authors":"Shunzhang Ma ,&nbsp;Hao Ran ,&nbsp;Jinquan Lin ,&nbsp;Yuanlin Sun ,&nbsp;Lizhen Zhang ,&nbsp;Fang Zhong ,&nbsp;Sumei Zhou ,&nbsp;Dianzhi Hou","doi":"10.1016/j.jspr.2026.103058","DOIUrl":"10.1016/j.jspr.2026.103058","url":null,"abstract":"<div><div>Cold plasma (CP) treatment, as a non-thermal stabilization strategy, is a promising alternative to heat technology for improving the storage stability of oat flour. In this study, the efficacy of CP treatment on enhancing the storage stability of oat flour was evaluated and the underlying mechanism of lipid oxidation and flavor evolution was explored via lipidomics and GC-MS analysis. Results showed that oats were treated under varying CP conditions, and 40 kV for 90 min was determined as the optimal parameter, which resulted in the inhibition rates of lipase, lipoxygenase, and peroxidase activities reaching 45.6%, 34.8%, and 56.0%, respectively. Furthermore, during 28-day accelerated storage, the activities of three enzymes in CP-treated oat flour were continuously inhibited, and their fatty acid value (9.3%), peroxide value (32.8%), and malondialdehyde content (17.9%) were significantly reduced when compared with the control group. The total phenolic content of oat flour was also remarkably retained by CP treatment. Untargeted lipidomics showed that CP inhibited lipid hydrolysis at the diacylglycerol and lysophosphatidylcholine stages, blocking free fatty acid release. Although CP transiently increased aldehydes, it ultimately reduced the off-flavor markers and promoted the accumulation of certain pleasant esters during storage. These findings demonstrated that CP enhanced the storage stability of oat flour by modulating lipid metabolic flux through irreversible enzyme inactivation.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"117 ","pages":"Article 103058"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioefficacy and antioxidant enzyme-modulating potential of jellyfish (Acromitus flagellatus) venom against the rice weevil (Sitophilus oryzae) and its application in an insect-resistant biopackaging formulation","authors":"V.D. Samaraweera ,&nbsp;T.M.S.U. Gunathilake ,&nbsp;A.G.W.U. Perera ,&nbsp;D.C.T. Dissanayake","doi":"10.1016/j.jspr.2026.103057","DOIUrl":"10.1016/j.jspr.2026.103057","url":null,"abstract":"<div><div>Recent global increases in jellyfish blooms have highlighted their potential as sources of bioactive compounds. Despite generally negative perceptions, jellyfish remain largely unexplored as sources of insect pest management agents. This study evaluates the insecticidal efficacy of <em>Acromitus flagellatus</em> venom and its application in developing insect-resistant biofilm. Venom extracted from the marginal lappets using phosphate-buffered saline (PBS) and Tris-buffered saline (TBS) was evaluated for its potential as bio-insecticidal agent against <em>Sitophilus oryzae</em> at 100%, 75%, and 50% concentrations. Insecticidal, repellent, and feeding-deterrent activities were assessed alongside biochemical responses linked to oxidative stress. Chitosan-based biofilms incorporating jellyfish venom, polyethylene glycol, and glutaraldehyde crosslinker were formulated at varying densities (F1–F5), with a control, and characterized for structural, physical, mechanical, and functional properties. Venom treatments exhibited significant concentration- and time-dependent increases in mortality (up to 98.33 ± 3.33%) and repellency (95.00 ± 6.38%), with the highest efficacy observed for PBS-extracted venom (p &lt; 0.05). Feeding activity and growth rates were markedly reduced. Biochemical analyses revealed oxidative stress induction, indicated by decreased reduced GSH levels and increased GSSG, catalase, and peroxidase activities. FTIR analysis confirmed successful incorporation of venom into the chitosan matrix, while increased crosslinking improved mechanical strength and reduced film transparency. Venom-incorporated biofilms exhibited enhanced tensile strength, sustained venom release, and prolonged repellency for up to 35 days. Overall, <em>A. flagellatus</em> venom shows promise as a novel bio-insecticidal agent and functional additive in bio-based packaging. Future work should address compound isolation, safety evaluation, and field-level validation.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"117 ","pages":"Article 103057"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}