Hongli Ren , Jiabao Wang , Guanlin Qian, Song Pan, Heran Xu, Miao Yan, Huanyu Wang, Guang Xin
{"title":"Effects of simulated transport vibration on storage quality and cell wall metabolism of Actinidia arguta","authors":"Hongli Ren , Jiabao Wang , Guanlin Qian, Song Pan, Heran Xu, Miao Yan, Huanyu Wang, Guang Xin","doi":"10.1016/j.jspr.2025.102738","DOIUrl":"10.1016/j.jspr.2025.102738","url":null,"abstract":"<div><div><em>Actinidia arguta</em> is rich in nutritional value and has a sweet and sour flavor. However, the postharvest transportation preservation of <em>A. arguta</em> still needs further improvement. Vibration damage to fresh fruit caused by post-harvest transport can significantly affect storage quality and result in quality loss. This study investigated the effects of vibration during transportation on storage quality (sensory, physicochemical, nutritional quality) and cell wall metabolism of <em>A. arguta</em>. The results indicated that simulated transport vibration facilitated the wrinkling of the fruit and the decline in firmness during storage, leading to a reduction in sensory scores at the end of storage. It also intensified decay and weight loss, while accelerating the degradation of vitamin C (Vc) due to vibration stress and mechanical damage. Additionally, the breakdown of original pectin and the generation of soluble pectin were promoted, accompanied by increased activity of PG, PME, and PL enzymes during storage. In conclusion, simulated transport vibration significantly expedited the quality deterioration of <em>A. arguta</em> during storage, resulting in a marked reduction in fruit quality. Therefore, minimizing transportation vibrations plays a crucial role in maintaining product quality during storage and transit in the supply chain logistics of <em>A. arguta</em>. This study provides a theoretical basis for the subsequent solution of the problems of the <em>A</em>. <em>arguta</em> after transport and promotes the sustainable development of <em>A. arguta</em> industry.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102738"},"PeriodicalIF":2.7,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of temperature and relative humidity on the combined efficacy of insecticides and diatomaceous earth for the control of Trogoderma granarium on rough rice","authors":"Muhammad Bukhari , Hafiz Azhar Ali Khan","doi":"10.1016/j.jspr.2025.102740","DOIUrl":"10.1016/j.jspr.2025.102740","url":null,"abstract":"<div><div>Binary combinations of insecticides with diatomaceous earth are gaining importance for the control of stored insect pests; however, efficacy of these combinations and subsequent control of insect pests are greatly affected by the abiotic factors under storage conditions. Here, we studied the effects of temperature and relative humidity on the effectiveness of binary combinations of pirimiphos-methyl, alpha-cypermethrin, thiamethoxam or spinetoram with diatomaceous earth against larvae of <em>Trogoderma granarium</em> on rough rice. Binary combinations of insecticides (at 0.1, 1 or 10 ppm) with diatomaceous earth (500 ppm) were evaluated at 25, 30 or 35 °C and 55 or 75 % relative humidity after 7, 14 and 21 d interval. Overall, the efficacy of binary combinations of pirimiphos-methyl, thiamethoxam or spinetoram with diatomaceous earth generally increased with the rise of temperature, while the opposite was noted in the cases of alpha-cypermethrin + diatomaceous earth combinations. With few exceptions, mortality of <em>T. granarium</em> larvae was lower at 75 % relative humidity than that observed at 55 % relative humidity level. Complete mortality (100 %) was observed at 35 °C in the combinations of pirimiphos-methyl or thiamethoxam (10 ppm) + diatomaceous earth (500 ppm) in both humidity levels; spinetoram (10 ppm) + diatomaceous earth (500 ppm) treatment at 55 % relative humidity, after 21 d of exposure. The results of progeny produced revealed that at each dose rate of the binary combinations, with few exceptions, the number of adult produced increased with rise in temperature in both relative humidity levels. Overall, more numbers of adults were recorded at 75 % relative humidity level than those observed at 55 %. In conclusion, variations in dose rates, temperature and humidity should be taken into consideration when binary combinations of insecticides with diatomaceous earth are included in the management plan for <em>T. granarium</em> in stored rice.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102740"},"PeriodicalIF":2.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioactive volatiles of Allium sativum and Brassica juncea for management of Tribolium castaneum and Corcyra cephalonica: Comprehensive in-silico and in-vitro analysis","authors":"Biswajit Horijan , Aditi Kundu , Suresh M. Nebapure , Abhishek Mandal , Neeraj Patanjali , Arkadeb Mukhopadhyay , D.K. Yadava , Anupama Singh","doi":"10.1016/j.jspr.2025.102736","DOIUrl":"10.1016/j.jspr.2025.102736","url":null,"abstract":"<div><div>Stored grain insects, <em>Tribolium castaneum</em> (red flour beetle) and <em>Corcyra cephalonica</em> (rice moth) pose significant threats to global food security. In spite of effectiveness of the conventional fumigants, huge concerns raised for adverse effect on environmental and human health, thereby prompting interest in exploitation of plant-derived volatile oils (VOs) as eco-friendly alternatives. The present study aimed to evaluate fumigant toxicity of nine VOs of <em>Myrtus communis</em> (McEO), <em>Citrus sinensis</em> (CsEo), <em>Melaleuca alternifolia</em> (MaEO), <em>Eucalyptus globulus</em> (EgEO), <em>Alllium sativum</em> (GEO), and <em>Brassica juncea</em> (MEO) (four varieties individually) against <em>T. castaneum</em> and <em>C. cephalonica</em>. The fumigant toxicity of VOs varied in the order of GEO > MEO > CsEO > McEO > EgEO > MaEO. Fumigant action of GEO exhibited significant mortality against adults of <em>T. castaneum</em> (LC<sub>50</sub> 1.05 μL/L air, 24 h) followed by MEO (LC<sub>50</sub> 4.90–20.27 μL/L air, 24 h), CsEO (LC<sub>50</sub> 11.92 μL/L air, 24 h), and McEO (LC<sub>50</sub> 28.10 μL/L air, 24 h). In case of <em>C. cephalonica</em> larvae, MEO (LC<sub>50</sub> 9.52–21.55 μL/L air, 72 h) showed the highest response, followed by McEO (LC<sub>50</sub> 33.76 μL/L air, 72 h), CsEO (LC<sub>50</sub> 41.47 μL/L air, 72 h), and GEO (LC<sub>50</sub> 29.16 μL/L air, 72 h). Gas-Chromatography-Mass-Spectrometry (GC-MS) analysis of the most effective GEO revealed presence of diallyl disulfide (62.02 ± 2.48 %) and allyl monosulfide (36.84 ± 2.64 %), whereas MEO indicated abundance of 2-pentenenitrile (78.23 ± 2.69 % to 92.99 ± 2.10 %) in PM 28 (PM28-MEO), PM 30 (PM30-MEO), Pusa Karishma (PK-MEO), and Pusa Jagannath (PJ-MEO), along with trace amounts of allyl isothiocyanate (AITC), 1-butene-4-isothiocyanate, and 5-cyano-1-pentene. The McEO mainly consisted of α-pinene (48.21 ± 2.65 %) and 1,8-cineole (26.86 ± 2.33 %), whereas CsEO was predominantly made up of <em>l-</em>limonene (92.27 ± 3.08 %). <em>In silico</em> modelling depicted favourable binding affinity of AITC with arylalkylamine N-acyltransferase (AANAT, −16.06 kcal/mol) of <em>T. castaneum</em> supported by a hydrogen bond, π-sulfur, π-alkyl, and two alkyl-alkyl interactions. Whereas, 2-pentenenitrile interacted with juvenile hormone esterase (JHE, −15.87 kcal/mol) of <em>C. cephalonica</em> attributed to a π-alkyl, alkyl-alkyl, and two hydrogen bonds, thus explaining remarkable fumigant action of MEO. Further, allyl monosulfide interacted with JHE (−10.25 kcal/mol) of <em>C. cephalonica</em> via five alkyl-alkyl interactions and <em>l-</em>limonene with AANAT (−14.87 kcal/mol) of <em>T. castaneum</em> through two π-alkyl, and six alkyl-alkyl interactions, both resulting in target protein inhibition. These findings highlight the potential of GEO and MEO as natural fumigants for their inclusion in integrated pest management strategies, off","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102736"},"PeriodicalIF":2.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of herbal ice cream parameters using response surface methodology and its storage studies","authors":"Shashank Shakya , Ankur Aggarwal , Dinesh Chandra Rai , Tarun Verma , Kunal Nagar , Aakash Singh , Balkrishna Singh","doi":"10.1016/j.jspr.2025.102721","DOIUrl":"10.1016/j.jspr.2025.102721","url":null,"abstract":"<div><div>This study aimed to optimize herbal ice cream formulation using response surface methodology and evaluate its physicochemical, sensory, and microbiological properties compared to a control. Herbal ice cream was optimized by varying herbal mix (1–4 %), milk fat (4–10 %), cane sugar (10–14 %), and soy lecithin (0.2–0.6 %) levels. The optimized and control samples were assessed for physicochemical parameters, sensory attributes, and microbiological counts. A 90-day storage study was conducted on the optimized formulation. The optimal formulation contained 4.0 % herbal mix, 9.68 % milk fat, 14.0 % cane sugar, and 0.47 % soy lecithin. The optimized sample showed higher total phenolic content, antioxidant activity, protein content, and inhibitory ability compared to the control. During 90-day storage, hardness, protein content, inhibitory ability, total phenolic content, and milk fat decreased while overrun, melting time, and total solids increased. This study demonstrates the potential of incorporating herbal extracts into ice cream formulations to develop functional dairy products with enhanced health benefits.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102721"},"PeriodicalIF":2.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Subzero temperature storage of fresh garlic: Scientific efforts and commercial success","authors":"Moo Hyun Park , Ho Moon Seog , Dong Sun Lee","doi":"10.1016/j.jspr.2025.102735","DOIUrl":"10.1016/j.jspr.2025.102735","url":null,"abstract":"<div><div>Fresh garlic high in soluble solids content has a low freezing temperature and is capable of staying at a supercooled state without ice crystallization even below the freezing point for long time. This unique characteristic, presumably conferred by high soluble carbohydrate composition, enables the garlic bulbs to be stored at subzero temperature of −1.5 to −5 °C with minimum quality change almost for a year. The benefits are inhibition of sprouting and decay along with an affordable wider control window of temperature and humidity compared to conventional chilled storage of -1–0 °C and 60–75 % relative humidity. Complete curing is prerequisite for safe application of the subzero temperature storage and varietal differences in freezing tolerance should also be considered. Commercial applications and practices of the subzero temperature storage can supply fresh garlic bulbs of good quality year round in the market contributing to alleviation of seasonal price fluctuations. This technology has potential to be applied to minimally processed products such as peeled garlic cloves, fresh cut slices, and paste. It may also be combined with other preservation techniques such as controlled atmosphere storage, modified atmosphere packaging, and active packaging tools.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102735"},"PeriodicalIF":2.7,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wisdom Richard Mgomezulu , Mthakati Alexander R. Phiri , Davis Muthini , Paul Thangata
{"title":"Determining optimal stock of grain for national food security in Malawi: A two objective grain sizing dynamic optimization approach","authors":"Wisdom Richard Mgomezulu , Mthakati Alexander R. Phiri , Davis Muthini , Paul Thangata","doi":"10.1016/j.jspr.2025.102734","DOIUrl":"10.1016/j.jspr.2025.102734","url":null,"abstract":"<div><div>Food insecurity remains an issue of great concern in Malawi. As such, the Government of Malawi through the National Food Reserve Agency (NFRA) and the Agricultural Development and Marketing Corporation (ADMARC) introduced the Strategic Grain Reserve (SGR) in 1979 to be able to store grain that can be used in responding to vulnerable households, during food shortages amidst climate change concerns. However, past estimations of how much grain the SGR is supposed to hold employed static models that did not consider the dynamic nature of food requirements presented by climate change and volatile food prices, let alone the duo objectives of achieving food security whilst minimizing storage costs. Through understanding the dynamic and evolving goals of the country, the current study employed dynamic optimization algorithms using GEKKO and Numpy libraries in Python. In terms of how much grain the SGR is supposed to hold, the study found that the optimal stocks to be held for 3 months with another 3 months lead time to mobilize grains under public-private-partnership capacity is 316,350 MT to effectively offset any historical shortfalls in supply, 674,178 MT for the emergency reserve and 191,267.9 MT as buffer stocks. Nonetheless, the country's physical storage space is not adequate and too costly to hold such a quantity of grain. The study therefore advises using futures contracts and virtual stock programs, such as grain banks, to ensure a swift and effective response in emergencies.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102734"},"PeriodicalIF":2.7,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of different sub-zero temperatures and relative humidities on moisture content and germination of stored canola","authors":"Abhinav Tiwari, Fuji Jian","doi":"10.1016/j.jspr.2025.102725","DOIUrl":"10.1016/j.jspr.2025.102725","url":null,"abstract":"<div><div>Storage of high-moisture canola remains a major challenge due to mold growth resulting in seed deterioration. While equilibrium moisture content (EMC) models have been widely applied to above-zero storage and drying conditions, no research has quantified EMC isotherm and germination under sub-zero temperatures. Isotherm and germination of canola, conditioned to 14–18 % moisture contents, were tested at temperatures from −25 to +30 °C and relative humidities (RHs) between 15 and 94 %. Multiple EMC models were evaluated for their predictive accuracy across the studied temperatures and RHs. The main factor influencing EMC of canola from −5 to −20 °C was the RH, and temperature had minimal influence. Both temperature and RH influenced the EMC when temperature was <span><math><mrow><mo>≤</mo></mrow></math></span> −20 °C. When RH was higher than 75 %, EMC of canola had an exponential increase with the increase of RH and/or decrease of temperatures. Seed germination remained high (≥80 %) at −5 to −15 °C, and RH had a minimum influence on the germination. At −20 and −25 °C and higher than 40 % RH, canola germination was reduced to less than 40 % after EMC was reached, indicating severe damage to the canola seeds. Peleg model yielded the best fit to the isotherm data at different sub-zero temperature ranges. These findings offer essential guidance for sub-zero grain storage and drying strategies, with implications for preserving seed viability and reducing post-harvest losses.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102725"},"PeriodicalIF":2.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Melatonin prolongs the shelf life of snow peas by regulating the antioxidant system","authors":"Shuwei Nian, Yongjia Yang, Ifra Iqrar, Yuwen Shi, Zhengjie Zhang, Kehan Wang, Yufen Xi, Shengming Sun, Liping Kou","doi":"10.1016/j.jspr.2025.102733","DOIUrl":"10.1016/j.jspr.2025.102733","url":null,"abstract":"<div><div>Postharvest snow peas (<em>Pisum sativum</em> var. <em>saccharatum</em>) exhibit rapid quality deterioration characterized by chlorophyll degradation, tissue wilting, and accelerated senescence under ambient storage conditions. To address this postharvest challenge, we investigated the efficacy of melatonin immersion treatments (50, 100, and 500 μmol L<sup>−1</sup>) combined with refrigerated storage at the 4 ± 0.5 °C for 42 days. Experimental protocols involved a 30 s immersion in melatonin solutions followed by air drying (25 °C, 60 min). Researches showed the reduction of the rot rate in snow peas with 100 μmol L<sup>−1</sup> melatonin treatment. Furthermore, its application effectively maintained good color and sensory quality, improved free radical scavenging power and metal reduction power, decreased reactive oxygen species levels such as H<sub>2</sub>O<sub>2</sub> and O<sub>2</sub>·<sup>-</sup>, and promoted ascorbic acid - glutathione cycle, consequently elevating the levels of antioxidants as well as antioxidant enzymes. The results demonstrated that melatonin could be used as a fresh-keeping technology to improve the value and prolong the storage time of snow peas.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102733"},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biochemical composition, amino acid and fatty acid profile changes of aquaculture feeds formulated from alternative ingredients: Long duration, storage temperature effects and ancillary FTIR confirmation","authors":"Parul Puri , Jaigopal Sharma , Rina Chakrabarti , Guddu Kumar , Avanish Kumar Shrivastav , Ram Singh","doi":"10.1016/j.jspr.2025.102731","DOIUrl":"10.1016/j.jspr.2025.102731","url":null,"abstract":"<div><div>Extrusion and non-extrusion based aquafeeds 1 and 2 are prepared. Duckweed, <em>Spirodela polyrhiza</em> is used as alternative feed ingredient for partial fishmeal-substitution in extruded-diet. Non-extruded fishmeal-diet is treated as control. Feeds are stored at four temperature conditions low (LT1 = −20 °C; LT2 = 4 °C), ambient (AT3), high temperature (HT4 = 45 °C). Bimonthly assessment of nutritional profile at variable temperature conditions for biochemical parameters moisture, ash, crude protein (CP), crude lipid (CL), carbohydrates (as nitrogen-free extract = NFE), gross energy (GE), amino acid (AA) and fatty acid (FA) profile is performed during six-month storage. Storage temperature, duration have significant effect (P < 0.05) on moisture, CP, CL, NFE and GE content of diets with significant decrease (P < 0.05) along assessment duration (0 to 180 days) and across storage temperatures (LT1<LT2<AT3<HT4) for CP, CL. Moisture content shows significant decrease (P < 0.05) through LT to HT condition across assessment regime for diet 1, with significant interaction effects for both diets. Significant difference in GE (P < 0.05) exists between LT and AT3, LT and HT4 for diet 1, between LT1 and HT4 for diet 2; higher GE at LT storages. Rendered oxidative, hydrolytic, thermal losses of dietary proteins and lipids is exhibited in AA, FA profiles. Significant loss from initial is noteworthy for total saturated, monounsaturated and polyunsaturated FAs (n3, n6) in both diets. Markedly, there is an overall decrease in essential, non-essential AAs. FTIR-spectra elucidates storage loss of proteins, lipids and moisture. These novel outcomes assess percentage loss of nutrients in storage diets elucidating significant nutritional profile changes. Storage at −20 °C with utilization up to two-months is suggestible for best possible dietary gains to fish and consumers. Cold-storage maintenance though may have its economic bearings specifically for small-scale farmers with limited stockpile thus storage regulation must deliberate cost-benefit values when amassing feed amounts for longer durations.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102731"},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sayeda S. Ahmed , Abdullah A. Zahra , Mohamed S. El Masarawy , Shimaa S.H. Khalil
{"title":"Application of carbon dioxide and nitrogen to control the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) in stored combs","authors":"Sayeda S. Ahmed , Abdullah A. Zahra , Mohamed S. El Masarawy , Shimaa S.H. Khalil","doi":"10.1016/j.jspr.2025.102728","DOIUrl":"10.1016/j.jspr.2025.102728","url":null,"abstract":"<div><div>The greater wax moth (<em>Galleria mellonella</em>) is a significant pest that damages stored wax combs, leading to major losses for beekeepers globally. This study explored a safe method for protecting wax without leaving harmful residues. The effects of modified atmospheres (MAs) with varying concentrations of CO<sub>2</sub> (60 %, 70 %, 80 %) and N<sub>2</sub> (97 %, 98 %) were tested on different life stages of the wax moth (eggs, larvae, and pupae) at 25 °C. The study also evaluated the treatment on a larger scale using a 100 L barrel, with LT<sub>50</sub> values determined for 80 % CO<sub>2</sub> and 98 % N<sub>2</sub> over different storage periods (10, 20, and 30 days). Results showed that a 6-day exposure to all treatments killed 100 % of eggs, larvae (4th-6th instars), and pupae, except for the 60 % CO<sub>2</sub> treatment, which required 7 days for full mortality of fourth instar larvae and pupae. Additionally, 98 % N<sub>2</sub> took 7 days, and 97 % N<sub>2</sub> took 9 days to kill all pupae. Sensitivity to MAs varied by life stage: eggs were most sensitive to CO<sub>2</sub>, while larvae were most sensitive to N<sub>2</sub>. On a large scale, CO<sub>2</sub> treatment resulted in 100 % reduction in treated larvae after 10 days, while N<sub>2</sub> treatment reached 80.64 % after 10 days, increasing to 90.32 % after 30 days. Wax weight reduction was minimal in CO<sub>2</sub>-treated samples (0.28 % after 10 days and 1.24 % after 30 days) compared to N<sub>2</sub>-treated samples (5.36 % after 10 days, 12.28 % after 30 days). The results suggest that MAs are a viable alternative to toxic gases for protecting wax combs from <em>G. mellonella</em> infestation while preserving wax quality.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102728"},"PeriodicalIF":2.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}