Journal of Stored Products Research最新文献

{"title":"Process optimization for microgreens-based dairy (lassi) beverage: Consumer acceptability, bioactive composition, and storage life using kinetic modelling","authors":"Mahendra Gunjal ,&nbsp;Atul Khalangre ,&nbsp;Jyoti Singh ,&nbsp;Sawinder Kaur ,&nbsp;Sezai Ercisli ,&nbsp;Emine Macit ,&nbsp;Prasad Rasane","doi":"10.1016/j.jspr.2025.102596","DOIUrl":"10.1016/j.jspr.2025.102596","url":null,"abstract":"<div><div>This study focuses on the development and optimization of a microgreens-based dairy beverage (lassi) using response surface methodology, aiming to enhance its bioactive composition, sensory acceptability, and storage stability. The optimal formulation, determined as 70% dahi and 2.5% microgreens powder with a desirability score of 0.818, significantly enriched the lassi with phenolics (114.00 mg GAE/100g), flavonoids (67.77 mg QUE/100g), anthocyanins (48.23 μmol/100g), and ascorbic acid (152.77 mg/100g), achieving an overall acceptability score of 8.60. Kinetic modeling evaluated the stability of bioactive compounds under storage conditions (5 °C, 15 °C, and 25 °C). At 5 °C, the highest retention of bioactive compounds, lower total plate count (5.69 log cfu/mL), and yeast and mold count (4.69 log cfu/mL) were observed after 15 days. The Arrhenius and Eyring models were applied to analyze temperature-dependent reaction rates, and the zero-order reaction model was combined with the Arrhenius model to predict shelf life. Shelf-life predictions at 5 °C were 21.875 days for overall acceptability, 12.696 days for acidity, 21.875 days for free fatty acids, 10.576 days for thiobarbituric acid, and 11.403 days for total plate count. The optimized lassi was found economically viable, with a production cost of ₹36.10 per 200 mL, aligning with market prices. These findings underscore the potential of radish sango microgreens for creating nutritionally superior, antioxidant-rich dairy beverages with broad commercial and health appeal.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102596"},"PeriodicalIF":2.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-coated liposomes of pomegranate-peel phenolic extract: Maximizing the bioaccessibility and antimicrobial activity in probiotic goat yoghurt","authors":"Malikakhon Kurbonova ,&nbsp;Marwa M. El-Said ,&nbsp;Samah M.S. EL-Shafei ,&nbsp;Victoria llina ,&nbsp;Hoda S. El-Sayed ,&nbsp;Wael M. Elmessery ,&nbsp;Mahmood A. Hashim ,&nbsp;Tamer M. El-Messery","doi":"10.1016/j.jspr.2025.102600","DOIUrl":"10.1016/j.jspr.2025.102600","url":null,"abstract":"<div><div>Chitosan-coated liposome was used to encapsulate pomegranate-peel phenolic extract (PPE). The liposome loaded with PPE demonstrated a notable encapsulation efficiency of 82.64 % and exhibited sizes ranging from 223.99 to 320.15 nm. The liposomal PPE displayed adequate antimicrobial activity depending on the concentration. Gram-positive strains appeared to be more sensitive. The probiotic strains exhibited a varied degree of extinction, with <em>L. plantarum</em> being the most resistant strain. Further evaluation was conducted after incorporating these liposomes into yoghurt, focusing on the changes observed during refrigeration storage. Optimization of the <em>in vitro</em> release behavior of milk beverages through response surface methodology (RSM), the physicochemical, microbiological, and sensory attributes of yoghurt containing PPE-loaded liposome at various concentrations (YF25, YF50, and YF75, representing 25, 50, and 75 mg phenolics per 100 g yoghurt) during the cold storage were evaluated. Results indicated YF25 exhibited the most favorable properties, resembling the control sample. All lactic bacteria strains loaded in yoghurt were enhanced for ten days of storage. The liposome loaded with PPE demonstrated stability under acidic conditions and resisted the stomach's harsh environment. The optimal conditions for the encapsulation process were achieved at encapsulation value of 0.289 or non-encapsulated value of 0.711 for YF75 and digest 1 (intestinal stage). Under these conditions, the responses for total phenolic content (TPC), together with total flavonoid content (TFC), and total antioxidant capacity (TAC) were measured at 9.677, 1.510, and 1208.421 μg of compound/g of extract, respectively. In contrast, the antioxidants increased approximately tenfold, as determined by the DPPH assay.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102600"},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of feed type, environmental conditions, and pesticide usage on the growth and aflatoxin production by Aspergillus parasiticus during feed storage","authors":"Jing Gao ,&nbsp;Qi Guo ,&nbsp;Jun Zhang ,&nbsp;Jiahui Li ,&nbsp;Peiya Wang","doi":"10.1016/j.jspr.2025.102599","DOIUrl":"10.1016/j.jspr.2025.102599","url":null,"abstract":"<div><div>The co-occurrence of AFB₁, AFB₂, AFG₁, and AFG₂ in mold-contaminated feed suggests a significant contribution of <em>A. parasiticus</em> to aflatoxin contamination in feed. In contrast to <em>A. flavus</em>, research on the effects of <em>A. parasiticus</em> growth and aflatoxin production is limited. This study aims to investigate the factors influencing the growth and aflatoxin production by <em>A. parasiticus</em>, as well as to analyze the interactions among these factors utilizing response surface methodology. The results will be compared with relevant studies on <em>A. flavus</em>, ultimately contributing to the development of a comprehensive strategy for the prevention of aflatoxin contamination. The findings indicated that both <em>A. parasiticus</em> and <em>A. flavus</em> used sugar as an initial substrate to produce aflatoxins. The substrate preference of <em>A. parasiticus</em> was not changed due to differences in nutrient content in different feeds. The mineral Mn was found to suppress aflatoxin production by both fungi, whereas glucose, sucrose, and Zn stimulated production. Temperature and water activity levels effective against <em>A. flavus</em> may not be equally effective for <em>A. parasiticus</em>. The optimum storage conditions were for temperature and water activity below 10 °C and 0.85 at the same time. Although chemical pesticides may comply with regulatory limits in feed, certain pesticides and their interactions could increase the risk of aflatoxin contamination during feed storage. Hence, a comprehensive evaluation of factors influencing both fungi is imperative to prevent aflatoxin contamination effectively.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102599"},"PeriodicalIF":2.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of pretreatment sterilization techniques and ginger (Zingiber officinale roscoe) essential oil-based active packaging on the quality of crucian carp (Carassius auratus) during cold storage","authors":"Peng Shi ,&nbsp;Jun Mei ,&nbsp;Jing Xie","doi":"10.1016/j.jspr.2025.102598","DOIUrl":"10.1016/j.jspr.2025.102598","url":null,"abstract":"<div><div>This study explored the effect of combining ginger (<em>Zingiber officinale</em> Roscoe) essential oil (GEO) active packaging with non-thermal sterilization techniques—ozonated water, slightly acidic electrolyzed water (SAEW), and photodynamic inactivation (PDI)—on the quality of crucian carp (<em>Carassius auratus</em>) during cold storage at 4 °C. The results showed that combining GEO with sterilization techniques significantly delayed microbial spoilage, extending the fish's shelf life from 9 days (control group) to 21–24 days. Microbiological analyses showed counts of total viable bacteria, <em>Pseudomonas</em> spp., H₂S-producing bacteria, and psychrotrophic bacteria in GEO compared to the control. The treated samples also exhibited lower total volatile basic nitrogen (TVB-N) values, and better water retention, as demonstrated by low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) analysis. Sensory evaluations further confirmed that the GEO-treated fish retained better odor, color, and texture during storage. In conclusion, the combined use of GEO and sterilization techniques proved highly effective in extending the shelf life of crucian carp while maintaining its quality, offering a promising solution for preserving perishable aquatic products.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102598"},"PeriodicalIF":2.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress and future trends in smart response packaging for food preservation","authors":"Xiaowei Xu ,&nbsp;Hanbin Chen ,&nbsp;Qin Wang ,&nbsp;Che Su ,&nbsp;Yilan Sun ,&nbsp;Chao Qiu ,&nbsp;Jie Pang","doi":"10.1016/j.jspr.2025.102597","DOIUrl":"10.1016/j.jspr.2025.102597","url":null,"abstract":"<div><div>Globally, it is estimated that about one third of the food destined for human consumption is wasted each year, a total of about 1.3 billion tons. This staggering amount of food waste is a serious problem in the food supply chain. However, the development and use of smart-responsive packaging materials has the potential to reduce food waste and extend shelf life. For example, some novel materials such as composite films with photocatalytic properties have been developed for fruit packaging, which can preserve strawberries for an extended period. However, existing smart packaging materials still face challenges in providing efficient, flexible and timely response. Furthermore, health threats from current packaging systems are a concern. For instance, residual chemicals from processing techniques and the excessive release of active molecules, such as some nanomaterials used in packaging, can threaten human health. Therefore, further development and optimization of smart packaging materials that can timely respond to changes in the storage micro-environment are crucial for food preservation. This article provides an overview of the latest advances in smart responsive packaging that employ multiple stimulus responses, summarizing the response mechanisms and application advances of temperature, humidity, gas, pH, and light smart responsive packaging. In addition, the article consider the challenges to further developing smart responsive packaging systems and suggests possible solutions. Responding to actual production needs, new approaches, such as material safety assessment and improved response sensitivity, can be considered in combination with artificial intelligence techniques to better design applicable smart packaging systems. These developments are important for reducing food waste, improving food shelf life, and ensuring consumer safety.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102597"},"PeriodicalIF":2.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications of mitonuclear interactions for the Trojan Female Technique in pest biocontrol: A case study on the seed beetle Acanthoscelides obtectus","authors":"Lea Vlajnić ,&nbsp;Filip Vukajlović ,&nbsp;Sanja Budečević ,&nbsp;Uroš Savković ,&nbsp;Biljana Stojković ,&nbsp;Mirko Đorđević","doi":"10.1016/j.jspr.2025.102595","DOIUrl":"10.1016/j.jspr.2025.102595","url":null,"abstract":"<div><div>The seed beetle (<em>Acanthoscelides obtectus</em>) is a pest of common beans and other legumes that causes considerable losses of stored products. Its populations are universally regulated with insecticides, but novel, safer and more efficient methods of control are needed. One such method is the Trojan Female Technique (TFT) which is based on the existence of male harming/female benign mitochondrial haplotypes. The introduction of females carrying haplotypes with male-specific negative effect into the target population can lead to its self-perpetuating suppression over generations. Due to the coevolution of mitochondrial and nuclear genomes, it is possible for nuclear variants that compensate for the negative effects of male harming mitochondrial haplotypes to evolve. Therefore, one of the requirements for the applicability of TFT is that candidate mitochondrial haplotypes confer their negative effects on males in diverse nuclear backgrounds. In this study, we used the previously described mitochondrial haplotype, MG3b, which reduces male-only fertility, and placed it in a range of nuclear backgrounds from three different natural populations. The goal was to test whether its effects on male and female fertility are dependent on interactions with different nuclei. Our results showed that MG3b consistently decreases male fertility independently of the nuclear environments, while having no negative effect on females. Additionally, we found no statistically significant effect of mitonuclear interactions, suggesting that there is no standing variation of compensatory mutations in nuclear backgrounds. These results indicate that the MG3b haplotype could be used in the TFT in different natural seed beetle populations.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102595"},"PeriodicalIF":2.7,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights into water balance disruption as a pest control strategy for Rhyzopertha dominica (F.)","authors":"Yueliang Bai ,&nbsp;Guiyao Wang ,&nbsp;Ru Zhang ,&nbsp;Chunqi Bai ,&nbsp;Jianhua Lv ,&nbsp;Meng Zhang","doi":"10.1016/j.jspr.2025.102593","DOIUrl":"10.1016/j.jspr.2025.102593","url":null,"abstract":"<div><div><em>Rhyzopertha dominica</em> (Fabricius), the lesser grain borer, is a major pest of stored grains and causes significant economic losses globally. Inert dust, an environmentally friendly insecticide, can cause rapid water loss and death in insects by disrupting the cuticular waterproof barrier. Its successful application highlights the potential of targeting the water balance as an effective pest control strategy, especially in dry storage environments. However, research on the molecular mechanisms of stored-product insects under inert dust or direct desiccation conditions is limited. In the present study, we assessed the lethal effects of inert dust (LC₅₀ = 0.606 g/m²) and desiccation (LT₅₀ = 6.208 d) on <em>R. dominica</em>. Subsequently, a high-quality gene set containing 14,363 protein-coding sequences was obtained through genome annotation. Comparative transcriptome analysis of the two treatments was conducted by mapping to a reference gene set, followed by RT-qPCR validation. The analysis revealed a substantial overlap in the DEGs between the two treatments. Among these, 141 upregulated genes were primarily involved in processes such as fatty acid elongation, uric acid metabolism, and phospholipid metabolism, whereas 183 downregulated genes were mainly associated with nutrient absorption, detoxification, and hormone regulation, reflecting an energy trade-off in <em>R. dominica</em> under stress conditions. Moreover, multiple key enzyme genes that participate in cuticular hydrocarbon biosynthesis were significantly upregulated, suggesting a critical role for this process in water stress adaptation. These findings provide valuable resources for molecular research on <em>R. dominica</em> and offer insights into the development of pest management strategies targeting insect water balance mechanisms.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102593"},"PeriodicalIF":2.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of functional beverage using pineapple juice and Bougainvillea spectabilis flower powder: Effect on the quality and storage stability","authors":"Pooja Mahey ,&nbsp;Maanas Sharma ,&nbsp;Manmath Sontakke ,&nbsp;Arun Kumar Gupta ,&nbsp;Avinash Kumar Jha ,&nbsp;Javed Masood Khan","doi":"10.1016/j.jspr.2025.102587","DOIUrl":"10.1016/j.jspr.2025.102587","url":null,"abstract":"<div><div>In this study, dried <em>Bougainvillea spectabilis</em> flower powder (0.25 g/50 mL to 4 g/50 mL) was used to develop a functional pineapple beverage. The enriched formulations showed enhanced physicochemical and bioactive properties. Titratable acidity ranged from 0.71% to 0.93%, higher than the control sample's 0.71%, indicating increased acidity, with pH values between 3.53 and 3.64. The enriched samples exhibited higher total soluble solids (TSS) compared to the control, reflecting improved compositional density. The bioactive analysis revealed a significant increase in total phenolic content (TPC), ranging from 89.81 mg GAE/mL to 101.83 mg GAE/mL, and total flavonoid content (TFC), rising from 11.77 mg QE/mL in the control to 12.53 mg QE/mL in the highest-enriched sample. Although the enriched formulations initially showed slightly lower antioxidant activity (23.04 ± 0.89%) compared to the control, their antioxidant stability was significantly higher during storage (<em>p&lt;0.05</em>). Sensory evaluation identified significant differences among samples, with the control and low-concentration formulations (T1: 0.25 g and T2: 0.5 g powder) receiving the highest overall acceptability scores of 8.5, 8.3, and 7.9, respectively. However, higher powder concentrations (e.g., T5: 1.25 g) negatively impacted sensory attributes, resulting in lower scores. These findings suggest that incorporating optimized amounts of <em>Bougainvillea spectabilis</em> flower powder enhances the nutritional, functional, and sensory properties of pineapple juice. This highlights its potential as a novel ingredient for functional beverage development, aligning with current nutraceutical trends.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102587"},"PeriodicalIF":2.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of β-hexosaminidases and α-mannosidases with changes in protein conjugated N-glycan profiles during peach ripening","authors":"Xia Fan ,&nbsp;Ting Wang","doi":"10.1016/j.jspr.2025.102592","DOIUrl":"10.1016/j.jspr.2025.102592","url":null,"abstract":"<div><div>Recent studies have demonstrated that N-glycan and its metabolic pathway are crucial in the ripening and softening processes of peaches, as evidenced by the significant upregulation of processing enzymes such as α-mannosidase (α-Man) and β-hexosaminidase (β-Hex). However, little is known about the changes of protein conjugated N-glycans in peaches during storage. In this study, a systematic comparison of the N-glycan profiles of three peach cultivars with distinct flesh types, specifically, the soft-melting “Hu Jing”, the hard-melting “Xia Hui 6″, and the stonyhard “Xia Cui” was provided. The study monitored the structure and content changes of these N-glycans during storage. Fourteen different N-glycan structures were identified using Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. A consistent N-glycan profile with varying relative content was observed among three cultivars. MMXF³, GnGnXF³, and MM were characterized as representative structures associated with ripening, showing statistically significant correlations with firmness. The conversion of GnGnXF³ to MMXF³ were proposed to be catalyzed by β-Hexs, which exhibited significantly increased activities in all three cultivars during storage. The degradation of high-mannose N-glycans to yield MM structure were observed, but no significant increase in α-Man activities was observed. Real-time qPCR results demonstrated that distinct gene expression patterns of β-Hexs and α-Mans existed among three cultivars. The expressions of genes hex0020 and man4915 were highest and exhibited a climacteric pattern in the “Hu Jing” cultivar. In contrast, these same genes displayed a double-peak expression pattern in the “Xia Hui 6” cultivar and steadily decreased in the “Xia Cui” cultivar. Our findings provide detailed insights into the changes of N-glycome associated with peach ripening and suggest potential target genes for preventing excessive softening in peaches. However, further investigation is needed to clarify the role of N-glycosylation and its related metabolic enzyme genes in peach fruit ripening and softening.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102592"},"PeriodicalIF":2.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of storage facilities in managing toxigenic fungi and mycotoxins associated with stored maize (Zea mays L.) in Ethiopia","authors":"Birhane Atnafu ,&nbsp;Alemayehu Chala ,&nbsp;Fikre Lemessa ,&nbsp;Wakuma Bayissa ,&nbsp;Abdi Mohammed ,&nbsp;Chemeda Abedeta Gerbaba","doi":"10.1016/j.jspr.2025.102561","DOIUrl":"10.1016/j.jspr.2025.102561","url":null,"abstract":"<div><div>Improved post-harvest handling of maize grain is an important activity to reduce fungal infection and mycotoxin contamination. This study aimed to evaluate the effectiveness of different storage facilities in managing toxigenic fungi and mycotoxins. The experiment was conducted from December 2020 to Augst 2021 at Jimma University with two storage types: storage bags (AgroZ®, fertilizer, jute, polypropylene, and PICS bag) for shelled maize, and <em>Gombisa</em> (traditional and modified) for maize cobs. <em>Gombisa</em> is cylindrical granary storage facility. Constant temperature and relative humidity were recorded in PICS, AgroZ®, and fertilizer bags, and consistent temperature in modified <em>Gombisa</em>. The toxigenic fungi detected in this study included <em>Aspergillus, Fusarium</em> and <em>Penicillium</em> species. Fungal infection incidence, grain germination and moisture content were highly significantly (P &lt; 0.001) influenced by the interaction of storage facilities and duration of storage in both experiments. The highest incidences of toxigenic fungi were recorded in polypropylene and jute bags after 2nd and 4th months in the traditional <em>Gombisa</em> respectively. There was a significant positive correlation (P &lt; 0.001) among storage duration and incidence of toxigenic fungi. Twenty-two mycotoxins were detected in the maize grains stored for eight months, with <em>Fusarium</em> metabolites being the most dominant. Fumonisins B1, B2, B3 and B4, and DON, ZEN and NIV were detected from loading time up to 8th month. Low fungal incidences, highest grain germination percentage, and low mycotoxin contamination were recorded in PICS, fertilizer bag and modified <em>Gombisa</em>. Thus, the study affirmed the effectiveness of PICS, fertilizer bags, and modified <em>Gombisa</em> in reducing mold and mycotoxin contamination under stored maize grain.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"111 ","pages":"Article 102561"},"PeriodicalIF":2.7,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}