Luisa Fernanda Zuluaga , Angelica Plata-Rueda , Luis Carlos Martínez
{"title":"Allium sativum essential oil against Sitophilus zeamais (Coleoptera: Curculionidae): composition, insecticidal activity, and behavioral response","authors":"Luisa Fernanda Zuluaga , Angelica Plata-Rueda , Luis Carlos Martínez","doi":"10.1016/j.jspr.2025.102943","DOIUrl":"10.1016/j.jspr.2025.102943","url":null,"abstract":"<div><div>The maize weevil, <em>Sitophilus zeamais</em>, is a globally distributed pest of stored grains. This study characterized the <em>Allium sativum</em> essential oil (EO) using gas chromatography–flame ionization detection (GC–FID) and gas chromatography–mass spectrometry (GC–MS), and evaluated its insecticidal activity against <em>S. zeamais</em>. The effects of the essential oil on weevil mortality, survival, food preference, and repellency were investigated. The major compounds identified were diallyl sulfide, diallyl disulfide, dimethyl trisulfide, allyl methyl disulfide, and diallyl tetrasulfide. In dose–mortality bioassays, the <em>A. sativum</em> EO (LD<sub>50</sub> = 0.033 μg insect<sup>−1</sup>), diallyl disulfide (LD<sub>50</sub> = 0.026 μg insect<sup>−1</sup>), and dimethyl trisulfide (LD<sub>50</sub> = 10.385 μg insect<sup>−1</sup>) were found to be lethal to <em>S. zeamais</em>. The survival rate was 99.9 % in untreated adults but decreased to 50.1 %, 46.7 %, and 45.6 % in weevils treated with LD<sub>50</sub> of <em>A. sativum</em> EO, dimethyl trisulfide, and diallyl sulfide, respectively. Furthermore, <em>A. sativum</em> EO and its compounds reduced the weevils’ preference for maize grains and exhibited repellent effects. These results indicate that <em>A. sativum</em> EO and its compounds disrupt various physiological and behavioral functions in <em>S. zeamais</em>. This study opens new perspectives for the control of stored-product pests and represents a preliminary step toward the development of green insecticides.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102943"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Petar Kljajić, Goran Andrić, Marijana Pražić Golić
{"title":"Impact of commercial phosphine fumigation of stored-grains in concrete silos on survival and progeny of natural free-living insect pests","authors":"Petar Kljajić, Goran Andrić, Marijana Pražić Golić","doi":"10.1016/j.jspr.2025.102895","DOIUrl":"10.1016/j.jspr.2025.102895","url":null,"abstract":"<div><div>This study was conducted to examine the impact of commercial phosphine fumigation on survival and progeny of natural free-living insect populations in wheat and maize stored in concrete silos. Aluminium phosphide pellets (560 g/kg) were applied at the recommended dose of 20–25 pellets/t of grains (4–5 g of phosphine gas/t) in two airtight cells in one silo at Pančevo locality (wheat and maize) and in one without airtight cell, in silos in Perlez (wheat) and Vrbas (maize). Samples collected before fumigation were used for pre-treatment assessment of grains' infestation levels, while samples taken for post-treatment assessment served to determine insect survival after 4 weeks and progeny after 8, 12 and 16 weeks. All taken samples were kept and analysed in laboratory. In pre-treatment assessment of wheat we found two primary and three secondary pests in density rates 0–0.9 and 1-34 adults/sample/kg, respectively. In maize were recorded one primary and three secondary pests in rates 1.5–1.7 and 0.9–62 adults/sample/kg, respectively. Post-treatment assessments of treated grains showed no insect survival and progeny. However, in untreated grains we recorded population growth of all insect species, especially of primary pests, <em>Rhyzopertha dominica</em> (F.) in wheat in Pančevo, from 0 to 170.4 adults/kg, and <em>Sitophilus zeamais</em> (Motsch.), in maize in Pančevo from 1.5 to 82.6 adults/kg and in Vrbas from 1.7 to 74.9 adults/kg. The phosphine gas high impact on survival and progeny of all recorded natural free-living insects, regardless of grain and storage type is promising for its further use in the future.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102895"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of factitious food on the life table, functional and numerical response parameters of Blaptostethus pallescens Poppius (hemiptera: Anthocoridae)","authors":"Mayur Deepak Wabhitkar , Manmeet Brar Bhullar , Arshpreet Singh , Azadeh Farazmand , Shiwani Sharma , Jenia Thakur","doi":"10.1016/j.jspr.2025.102888","DOIUrl":"10.1016/j.jspr.2025.102888","url":null,"abstract":"<div><div>The present study evaluates the life table, functional and numerical response of <em>Blaptostethus pallescens</em> (Poppius) when reared on three different factitious preys: <em>Tyrophagus putrescentiae</em> (Schrank), <em>Corcyra cephalonica</em> (Stainton), and <em>Phthorimaea operculella</em> (Zeller). Significant variation was observed in developmental time, adult longevity, and reproductive parameters across the three diets. Individuals fed on <em>T. putrescentiae</em> exhibited prolonged pre-adult development and reduced adult longevity and fecundity compared to those fed on lepidopteran eggs. Female fecundity was highest on <em>C</em>. <em>cephalonica</em> and <em>P. operculella</em> eggs, while lowest on <em>T. putrescentiae</em>. Life table analysis revealed that the intrinsic rate of increase (r) was significantly lower on <em>T. putrescentiae</em>, indicating suboptimal population growth, whereas lepidopteran egg diets promoted higher reproductive and population parameters. Functional response analysis confirmed a type II response across all prey, with the highest attack rate (3.5 h<sup>−1</sup>) and shortest handling time (0.0079 h) recorded on <em>T. putrescentiae</em>. Despite lower fecundity, <em>T. putrescentiae</em> supported full development and survival of <em>B. pallescens</em>, suggesting its potential as a factitious prey in mass rearing systems. This predator completed its life cycle inside breadboxes containing wheat bran and yeast, indicating feasibility for use in predatory sachets. These findings support the use of <em>B. pallescens</em> in biological control programs, particularly in greenhouse systems, where cost-effective and locally adaptable rearing methods are urgently needed. The study also highlights the promise of <em>T. putrescentiae</em> as a prey species for slow-release systems, offering a practical solution for enhancing macrobial biocontrol strategies in India. Although <em>T. putrescentiae</em> is known to produce allergens, the adoption of simple safety measures, such as wearing masks and gloves can effectively minimize any associated risks during handling or rearing.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102888"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sreelakshmi Mullachery, Naduvilthara U. Visakh, Berin Pathrose
{"title":"Valorization of guava leaf waste: Chemical characterization and comparative bioefficacy of Psidium guajava L. essential oil against key stored-product insect pests","authors":"Sreelakshmi Mullachery, Naduvilthara U. Visakh, Berin Pathrose","doi":"10.1016/j.jspr.2025.102935","DOIUrl":"10.1016/j.jspr.2025.102935","url":null,"abstract":"<div><div><em>Psidium guajava</em> (Myrtaceae), commonly referred to as guava, is a tropical plant widely cultivated across the tropics and subtropics for its fruits. The plant is also characterized by anti-inflammatory, anti-microbial, anti-oxidant and anti-diabetic properties. However, the training and pruning waste of these trees is often discarded as agricultural waste. This study was conducted to extract essential oil from dried, ground guava leaves through hydro-distillation, identify its chemical constituents through GC-MS analysis, and investigate the potential of using guava leaf oil as a biopesticide against three key stored grain pests<em>, Tribolium castaneum, Sitophilus oryzae</em> and <em>Rhyzopertha dominica.</em> The leaves yielded 1.4 ± 0.01 % oil, caryophyllene (24.43 %), D-limonene (16.13 %) and α-bisabolene (8.13 %) were identified as the major chemical components and the oil demonstrated excellent contact (LC<sub>50</sub> at 24 h was 7.92 mg/cm<sup>2</sup> for <em>T. castaneum,</em> 12.71 mg/cm<sup>2</sup> for <em>S. oryzae</em> and 5.41 mg/cm<sup>2</sup> for <em>R. dominica</em>), fumigant (LC<sub>50</sub> at 24 h was 18.91 μL/L air for <em>T. castaneum,</em> 33.25 μL/L air for <em>S. oryzae</em> and 11.25 μL/L air for <em>R. dominica</em>) and repellent activity. The highest mean repellency was exhibited against <em>T. castaneum</em> at 0.09 % across varying exposure periods. An area preference technique was used to evaluate the repellent activity. Additionally, a phytotoxicity test on wheat seeds showed no significant effect on seed germination or seedling growth. Thus, guava leaf waste generated during training and pruning can be used to extract leaf oil, which can then be used as a safe botanical pesticide to control storage pests.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102935"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abhishek Dasore , Norhashila Hashim , Rosnah Shamsudin , Hasfalina Che Man , Maimunah Mohd Ali , Opeyemi Michael Ageh
{"title":"Evaluation of quality attributes and milling metrics of glutinous rice stored under different storage conditions using infrared thermal imaging","authors":"Abhishek Dasore , Norhashila Hashim , Rosnah Shamsudin , Hasfalina Che Man , Maimunah Mohd Ali , Opeyemi Michael Ageh","doi":"10.1016/j.jspr.2026.102960","DOIUrl":"10.1016/j.jspr.2026.102960","url":null,"abstract":"<div><div>Infrared thermal imaging (ITI) has emerged as a promising tool for non-destructive evaluation of agricultural product quality. This study investigates the application of ITI for monitoring the quality attributes and milling metrics of glutinous rice (GR) during storage under varying conditions. GR samples were first dried at 50 °C, 60 °C, and 70 °C, then stored at freezing (−10 °C), cold room (6 °C), and ambient (26 °C) temperatures for 6 months (24 weeks). Thermal images (TI) of the GR were acquired biweekly to evaluate the changes in key quality indicators, including moisture content (MC), germination growth rate (GGR), water absorption capacity (WAC), whiteness index (WI), head rice yield (HRY), and broken rice yield (BRY). Relevant features were extracted from the TI and correlated with the corresponding physicochemical properties of the GR. Ten machine learning (ML) algorithms were tested to predict the quality attributes of GR based on the features extracted from TI data. Among them, the ET model demonstrated superior performance compared to the others. Its predictive capability was further enhanced through grid search (GS) hyperparameter tuning (HPT), achieving an R<sup>2</sup> of 0.939 and an RMSE of 0.178 for MC. The accuracy and reliability of the ET model were further supported by parity plots. Overall, the findings underscore the potential of integrating ITI with ML for non-destructive, real-time monitoring of GR quality, offering valuable insights for optimizing postharvest handling and storage practices.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102960"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pratibha Jain , Mohammed Shafiq Alam , Manpreet Kaur Saini , Raouf Aslam
{"title":"Recent technological innovations and strategies for reducing post-harvest losses during bulk grain storage: Applications of IoT and non-destructive quality evaluation","authors":"Pratibha Jain , Mohammed Shafiq Alam , Manpreet Kaur Saini , Raouf Aslam","doi":"10.1016/j.jspr.2025.102893","DOIUrl":"10.1016/j.jspr.2025.102893","url":null,"abstract":"<div><div>Ensuring food security, minimizing post-harvest losses, and improving grain storage methods are crucial for sustaining agricultural production. Currently, grain storage mismanagement contributes to significant losses, especially in developing countries. This review provides an in-depth analysis of global grain storage systems, traditional and modern technologies, and the critical factors influencing grain quality. It provides a recent report on the quantitative and qualitative losses caused by environmental factors, biological agents, and socio-economic conditions. Moreover, the study examines advancements in storage technologies, such as hermetic storage as a part of dry chain, bulk systems, and the integration of IoT for monitoring grain storage conditions. Through this comprehensive overview, the review offers solutions to optimize storage practices and reduce food losses, thereby contributing to food security and economic sustainability.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102893"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Thermal, non-thermal, and non-fumigant technologies for disinfestation of Callosobruchus species in stored pulses","authors":"Harshavardhan Reddy Settipalli, Snehasis Chakraborty, Bhadriraju Subramanyam, Kaliramesh Siliveru","doi":"10.1016/j.jspr.2025.102897","DOIUrl":"10.1016/j.jspr.2025.102897","url":null,"abstract":"<div><div>Management of <em>Callosobruchus</em> species in stored grains is a critical issue in the food industry. These species damage stored grains both qualitatively and quantitatively, which is detrimental to feeding the growing human population; therefore, these pests need to be properly controlled. The available disinfestation methods are effective. However, concerns exist over residues, environmental risks, and resistance to chemicals used for pest management. Therefore, environmentally friendly methods should be considered as alternatives. The disinfestation techniques, including dielectric (microwave and radio frequency) heating, low-temperature and high-temperature treatments, solar heating, steam heating, ozone treatment, controlled atmosphere, gamma irradiation, and cold plasma treatment, are discussed for stored pulses. Non-thermal methods for pest control are being studied worldwide as suitable alternatives. Therefore, it is crucial to investigate the mode of action of non-thermal methods for inactivation, as well as the impact on resistant and susceptible insect life stages. The effect on quality factors and shelf life of treated pulses is presented in this review. This paper highlights the importance of identifying the resistant insect life stages, applying kinetic modeling to optimize lethal time, and evaluating long-term storage performance. Establishing standardized benchmarks for mortality, seed viability, and nutritional retention will be essential to scale these technologies for commercial adoption.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102897"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145748253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Balasubramaniyan , J. Shavani Durga , V. Chelladurai , T. Senthilkumar , T. Krishnakumar , G. Jeevarathinam
{"title":"Aflatoxin detection and quality assessment in dried red chilli (Capsicum annum L.): A multifaceted approach using physical, microstructural, and machine learning techniques","authors":"M. Balasubramaniyan , J. Shavani Durga , V. Chelladurai , T. Senthilkumar , T. Krishnakumar , G. Jeevarathinam","doi":"10.1016/j.jspr.2026.102973","DOIUrl":"10.1016/j.jspr.2026.102973","url":null,"abstract":"<div><div>India contributes nearly 43 % of global dried red chilli production; however, inadequate post-harvest handling and poor storage conditions frequently lead to fungal contamination and nutritional degradation. Dried chillies are highly susceptible to infection by <em>Aspergillus flavus</em> and <em>A. parasiticus</em>, which produce aflatoxins, a serious food safety concern during storage. This study investigated the physicochemical and microstructural deterioration of healthy and infected red chillies and explored an objective image-based grading approach to minimize contamination. Whole chilli samples were analysed for physical attributes such as shape, size, mass, bulk density, and surface characteristics, while powdered samples were evaluated for water activity, colour, ash content, microbial load, and compositional changes using Fourier Transform Infrared (FTIR) spectroscopy. Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray (EDAX) analyses revealed distinct microstructural and elemental differences between healthy and infected samples, with the latter showing higher water activity and microbial counts indicative of fungal growth. FTIR spectra of infected and market samples exhibited characteristic peaks at 1548 cm<sup>−1</sup>and 1711 cm<sup>−1</sup>, confirming aflatoxin-associated functional groups (C=O stretching and amide II vibrations). To enhance pre-storage sorting efficiency, a deep learning model (YOLOv8m) was developed to classify and grade red chilli pods based on colour and morphology, achieving 92 % accuracy. This integrated approach provides a rapid, non-destructive method to identify and remove contaminated pods, thereby reducing aflatoxin risk and improving the safety and storability of dried chillies.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102973"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Surface-modified halloysite nanotubes for sustained release of clove bud essential oil in insect-repellent materials","authors":"Si-hoon Jang , No-Hyung Park , Su-il Park","doi":"10.1016/j.jspr.2026.102977","DOIUrl":"10.1016/j.jspr.2026.102977","url":null,"abstract":"<div><div>Halloysite nanotubes (HNTs) are naturally occurring aluminosilicate nanomaterials with lumen–surface polarity that enable encapsulation and sustained release of volatile compounds. In this study, clove bud essential oil (CBO), which exhibits strong insect-repellent activity, was encapsulated into HNTs via a vacuum-assisted loading process. To improve release control, HNTs loaded with CBO (denoted as HNCs) were subjected to sealed-end treatment and surface modification before being incorporated into gravure inks and printed onto linear low-density polyethylene (LLDPE) films. CBO consisted mainly of eugenol (64.3 %), β-caryophyllene (14.2 %), and eugenol acetate (10.8 %). Zeta potential shifted from −21.1 mV for pristine HNTs to near neutrality at −0.09 mV for HNCs with surface modification. Thermogravimetric analysis and TEM confirmed successful encapsulation and structural modification. Repellency tests against <em>Plodia interpunctella</em> larvae showed that all HNCs exhibited insect-repellent effects, with HNCs with surface modification maintaining the highest and most stable repellency (IR > 0.6) for 29 days. Release studies demonstrated that surface-modified HNCs delayed CBO release, with HNCs with surface modification showing the most controlled profile and reaching 66 % at day 32. Incorporation into LLDPE–HNCs with surface modification delayed release to 73 % at day 32. Sensory evaluations revealed no significant differences between the control LLDPE film and the LLDPE–HNCs with surface modification film in intensity attributes and hedonic preference (p > 0.05). These results confirm that surface-modified HNCs are effective carriers for sustained release of CBO in insect-repellent packaging.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102977"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thi Khanh Van Pham , Hoang Anh Thy Do , Ly Khanh Trinh , Duc Tri Luu , Long Phuoc Lieu , Dinh Quan Nguyen
{"title":"Active chitosan-alginate films incorporating methyl eugenol for improved food packaging functionality and postharvest preservation performance","authors":"Thi Khanh Van Pham , Hoang Anh Thy Do , Ly Khanh Trinh , Duc Tri Luu , Long Phuoc Lieu , Dinh Quan Nguyen","doi":"10.1016/j.jspr.2026.102988","DOIUrl":"10.1016/j.jspr.2026.102988","url":null,"abstract":"<div><div>The development of biodegradable active packaging materials is essential for improving food preservation while reducing reliance on petroleum-based plastics. In this study, chitosan–alginate composite films were prepared and functionalized with eugenol and its methylated derivative, methyl eugenol, and systematically evaluated in terms of structural, physicochemical, antimicrobial, antioxidant, biodegradation, and postharvest preservation performance. Structural analyses revealed strong intermolecular interactions and a reduction in film crystallinity upon bioactive incorporation, with a more pronounced amorphization effect observed for methyl eugenol. Compared with the control film, the methyl eugenol-loaded film exhibited significantly higher tensile strength (6.99 ± 0.21 MPa vs. 4.57 ± 0.17 MPa, p < 0.05) while maintaining moderate elongation at break (34.87 ± 1.13%). Water vapor permeability was significantly reduced by 28.4% (p < 0.05), indicating improved moisture barrier properties. Antioxidant assays demonstrated enhanced radical scavenging activity, while antimicrobial evaluation against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em> showed significant bacterial inhibition (p < 0.05), with methyl eugenol exhibiting the strongest effect. Soil burial tests confirmed that all films remained biodegradable, with more than 65% weight loss observed after 28 days. Postharvest application was evaluated using cherry tomatoes (n = 30 per treatment) stored under ambient conditions (25 ± 2 °C) for up to 12 days. The methyl eugenol-functionalized films significantly reduced decay incidence and weight loss and better preserved soluble solids, titratable acidity, and antioxidant capacity compared with the control and eugenol-containing films (p < 0.05). These findings demonstrate that methyl eugenol enhances both film functionality and postharvest preservation performance, highlighting its potential for sustainable active food packaging applications.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102988"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147394307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}