Journal of Stored Products Research最新文献

{"title":"Effectiveness of pirimiphos-methyl, alpha-cypermethrin, spinetoram and thiamethoxam alone and in combination with diatomaceous earth for the control of Trogoderma granarium on rough rice","authors":"Muhammad Bukhari ,&nbsp;Hafiz Azhar Ali Khan","doi":"10.1016/j.jspr.2025.102675","DOIUrl":"10.1016/j.jspr.2025.102675","url":null,"abstract":"<div><div>The khapra beetle, <em>Trogoderma granarium</em> Everts, is a serious threat to stored commodities with the potential to develop tolerance to insecticides. Binary combinations of insecticides with diatomaceous earth are usually considered effective grain protectants for the control of <em>T. granarium</em>. However, studies that directly compare their efficacy in rough rice are few. We examined control efficacy of pirimiphos-methyl, alpha-cypermethrin, spinetoram and thiamethoxam (at 0.1, 1 or 10 ppm) alone or in binary combinations with diatomaceous earth (DE; at 500 or 1000 ppm) against <em>T. granarium</em> on rough rice grains. The highest mortality (100 %) of <em>T. granarium</em> larvae was observed at the combination of pirimiphos-methyl (10 ppm) + DE (1000 ppm) after 14 days of exposure, and at the combinations of pirimiphos-methyl (1 ppm) + DE (1000 ppm); alpha-cypermethrin or spinetoram (10 ppm) + DE (1000 ppm) after 21 days of exposure. The mixture of thiamethoxam (10 ppm) + DE (1000 ppm) resulted in 90 % mortality of <em>T. granarium</em> after 21 days of exposure. Except thiamethoxam, mixtures of insecticides and DE resulted in complete suppression of F1 and F2 progeny at their highest rates. Moreover, after 90 days of exposure, the mixtures of insecticides with DE proved more effective in reducing grain weight loss than their individual applications. In conclusion, the data of the present study revealed that the binary combinations of insecticides and DE are likely to help manage <em>T. granarium</em> in stored rice.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102675"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Storage stability and packaging impact on spray-dried encapsulated Sesbania flower extract during long-term storage","authors":"Sochannet Chheng ,&nbsp;Mohammad Fikry ,&nbsp;Saeid Jafari ,&nbsp;Dharmendra K. Mishra ,&nbsp;Kitipong Assatarakul","doi":"10.1016/j.jspr.2025.102660","DOIUrl":"10.1016/j.jspr.2025.102660","url":null,"abstract":"<div><div>This study investigated the storage stability of spray-dried Sesbania flower extract (SFE) under different packaging conditions over six months. Microencapsulation was performed using gum arabic (GA), resistant maltodextrin (RMD), and their combination (RMDGA) at 150 °C and 160 °C, with GA at 160 °C identified as the optimal condition. The microcapsules exhibited high retention of total phenolic content (TPC) (16.86 mg GAE/g db), total flavonoid content (TFC) (3.29 mg QE/g db), and antioxidant activities (DPPH: 24.63 μM Trolox/g db; FRAP: 39.01 μM Trolox/g db), significantly higher than in other formulations (<em>p</em> ≤ 0.05). The effects of vacuum-sealed and normal-sealed aluminum and HDPE packaging were assessed during six-month storage. Vacuum-sealed aluminum bags provided the best protection, with TPC, TFC, DPPH, and FRAP retention of 70.5%, 51.4%, 53.2%, and 72.2%, respectively (<em>p</em> ≤ 0.05), while solubility and color remained stable. In contrast, HDPE packaging led to greater moisture absorption and faster bioactive degradation. Microbiological analysis confirmed the absence of pathogenic microorganisms, including <em>Salmonella</em> spp., <em>Listeria monocytogenes</em>, and <em>Staphylococcus aureus</em>, ensuring product safety during storage. This study highlights the importance of optimized packaging in maintaining bioactivity and extending the shelf life of encapsulated plant extracts for functional food applications.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102660"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yellowing and overall quality of ‘Legacy’ broccoli: 1-butanol vapor treatment as an alternative to 1-MCP during storage at room temperature","authors":"Fabio Rodrigo Thewes ,&nbsp;Mateus Gaiardo dos Santos ,&nbsp;Bruna Regina Carvalho Pinto ,&nbsp;Guilherme Zanon Peripolli ,&nbsp;Luise Victoria Agnes ,&nbsp;Kaliandra Zottele Nunes ,&nbsp;Lilian Osmari Uhlmann ,&nbsp;Daniel Alexandre Neuwald ,&nbsp;Auri Brackman ,&nbsp;Roger Wagner ,&nbsp;Vanderlei Both","doi":"10.1016/j.jspr.2025.102669","DOIUrl":"10.1016/j.jspr.2025.102669","url":null,"abstract":"<div><div>This study aimed to evaluate the impact of 1-butanol vapor treatment and compare it with ethanol, 1-methylcyclopropene (1-MCP), and ethylene treatments on ethylene production, respiration rate, color changes, physicochemical quality, and the volatile compounds derived from ethanol and 1-butanol during six days of storage at 20 °C. The florets were treated for 24 h at 20 °C in airtight chambers. After treatment, the florets were stored at 20 °C for six days, and metabolism and quality analyses were conducted after zero, three, and 6 days at 20 °C. Treating ‘Legacy’ broccoli florets with 1-butanol and 1-MCP increased ACC oxidase activity, ethylene production, and respiration rate while preserving better stem firmness and chemical quality, indicating that ethylene action is inhibited by these treatments. 1-Butanol is an effective alternative to delay the yellowing of ‘Legacy’ broccoli, maintaining better overall quality than 1-MCP and ethanol vapor treatments during six days of storage at 20 °C. 1-Butanol was more effective than ethanol in delaying yellowing in ‘Legacy’ broccoli. The delayed yellowing observed with 1-butanol treatment resulted from higher concentrations of chlorophyll and pheophytin maintenance. The application of 1-butanol and ethanol to ‘Legacy’ broccoli was dissipated differently by the florets; ethanol was primarily volatilized into the surrounding environment, whereas 1-butanol was bioconverted into butanal and butanoic acid and also volatilized into the surrounding environment. Lastly, the results demonstrated the potential of 1-butanol as an alternative treatment to control yellowing in ‘Legacy’ broccoli stored at 20 °C.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102669"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contact toxicity of insecticides against adults and larvae of Cadra cautella (Walk.) and Oryzaephilus surinamensis (L.)","authors":"Sangeeta K,&nbsp;Suresh M. Nebapure,&nbsp;Rajna S,&nbsp;C.N. Rajarushi,&nbsp;Ranjitha S. M,&nbsp;S. Subramanian","doi":"10.1016/j.jspr.2025.102667","DOIUrl":"10.1016/j.jspr.2025.102667","url":null,"abstract":"<div><div>Effective insecticide options for managing stored-product pests are increasingly limited, necessitating the exploration of alternatives to conventional insecticides such as malathion and deltamethrin. The saw-toothed grain beetle, <em>Oryzaephilus surinamensis</em> and almond moth, <em>Cadra cautella</em> are major storage pests that cause substantial quantitative and qualitative losses to stored grains and processed products. However, limited studies have evaluated the contact toxicity of alternative insecticides against different life stages of these pests. This study aimed to assess the efficacy of four insecticides <em>viz.,</em> lambda-cyhalothrin, spinosad, spinetoram, and chlorfenapyr against both adult and larval stages of <em>O. surinamensis</em> and <em>C. cautella</em> through laboratory bioassays at 24, 48, and 72 h of exposure. Among the tested insecticides, spinetoram exhibited the highest toxicity against <em>C. cautella</em> adults, with relative toxicity values of 2.43 and 3.13 compared to deltamethrin and malathion, respectively. Lambda-cyhalothrin was the most toxic to <em>O. surinamensis</em> adults, with relative toxicity values of 55.23 and 9.23. Larvae showed greater susceptibility than adults, with spinetoram displaying the highest toxicity against <em>C. cautella</em> larvae (42.82 and 9.56 relative toxicity), while lambda-cyhalothrin was most toxic to <em>O. surinamensis</em> larvae (21.23 and 10.04 relative toxicity). These findings highlight the potential of spinetoram and lambda-cyhalothrin as effective alternatives to conventional insecticides for pest management in bulk grain storage.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102667"},"PeriodicalIF":2.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of complex cryoprotectant on the freeze-drying survival of Lactobacillus acidophilus FMNS-10 and its protective mechanisms","authors":"Xiangfei Li,&nbsp;Menghan Wang,&nbsp;Meng Ding,&nbsp;Xinyi Pang,&nbsp;Jing Sun,&nbsp;Yingjian Lu","doi":"10.1016/j.jspr.2025.102646","DOIUrl":"10.1016/j.jspr.2025.102646","url":null,"abstract":"<div><div>Freeze-drying is a common method for preserving bacteria cells, but it can cause cell damage in the process of protection. Therefore, it is necessary to explore a cryoprotectant to improve cell survival during the process. This research aimed to explore the optimal cryoprotectant formulation of <em>L. acidophilus FMNS-10</em> and its protection mechanism. The optimal cryoprotectant consisted of 10.7 % skim milk, 3 % sodium L-glutamate and 10 % D-trehalose, resulting in a survival rate of 91.69 % for <em>L. acidophilus FMNS-10</em> after freeze-drying (<em>p</em> &lt; 0.05). Under this condition, the integrity of cell wall and membrane of <em>L. acidophilus FMNS-10</em> was close to that of the fresh strain, which could be attributed to preventing ice crystal formation. Cryoprotectants demonstrated a significant protective effect on enzymatic activities. In the cryoprotectant-free group, the activities of β-galactosidase, L-LDH, Na⁺/K⁺-ATPase and the concentration of Ca²⁺ decreased by 36.19 %, 51.82 %, 89.40 %, and 41.90 %, respectively. However, the cryoprotectant group were only 7.10 %, 1.66 %, 7.41 %, and 4.10 % reductions compared to the cryoprotectant-free group (<em>p</em> &lt; 0.05). These results indicated that the optimal cryoprotectant improved the survival rate of <em>L. acidophilus FMNS-</em>10 by inhibiting the loss of enzyme activity. In addition, the optimal cryoprotectant significantly mitigated the DNA damage induced by freeze-drying. The reduction in enzyme and DNA damage may be attributed to complex cryoprotectants maintaining the stability of their three-dimensional structures and reducing structural disruption caused by freezing. Further analysis of storage stability revealed that the viable count of <em>L. acidophilus FMNS-10</em> exceeded 10⁷ CFU/mL when stored at −20 °C for 180 days. These findings provide a comprehensive theoretical foundation for how to freeze-dry <em>Lactobacillus acidophilus.</em></div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102646"},"PeriodicalIF":2.7,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of silica on stored product pest, Sitophilus oryzae L. (Coleoptera: Curculionidae) and its residual impact on Triticum aestivum L. grain","authors":"Parthiban Packirisamy ,&nbsp;Baharani Soren ,&nbsp;T. Geetha ,&nbsp;C.S. Vivek Babu ,&nbsp;S. Ezil Vendan","doi":"10.1016/j.jspr.2025.102664","DOIUrl":"10.1016/j.jspr.2025.102664","url":null,"abstract":"<div><div>Wheat (<em>Triticum aestivum</em> L.) is a crucial global staple crop. Still, post-harvest losses caused by insect pests, such as the rice weevil (<em>Sitophilus oryzae</em> L.), significantly compromise grain quality and storage. Conventional pest control methods, particularly chemical insecticides, pose environmental and health risks, highlighting the urgent need for safer alternatives. This study assessed the efficacy of silicon nanoparticles in controlling weevil infestations and investigated their residual and physicochemical effects on wheat grains. Laboratory experiments were conducted using silicon powder at various concentrations, with untreated grains as control. Over 15 days, mortality rates, grain weight loss, and damage were recorded. The results demonstrated that higher concentrations of silicon, particularly 700 and 1000 ppm, achieved complete pest mortality within 5 days. Both weight loss and grain damage were significantly reduced at these concentrations, with the lowest rates observed at 0.50 % for weight loss and 0.44 % for damage at 1000 ppm. Additionally, the study examined the physicochemical properties of the silicon-treated grains, including moisture content, bulk density, porosity, and water uptake. The measurements were taken on the first day and again on the 180^th day of storage. While storage-induced changes were noted in treated and untreated grains, no adverse impacts on grain quality were identified. Residual silicon analysis using scanning electron microscopy-energy dispersive X-ray spectroscopy confirmed effective removal following double washing, ensuring the treated grains comply with safety standards. In conclusion, the findings suggest silicon nanoparticles represent a promising, residue-free, and environmentally sustainable approach to post-harvest pest management, effectively preserving grain quality while mitigating pest-induced losses.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102664"},"PeriodicalIF":2.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation on compression bruised mechanism of Cyperus esculentus L based on experiment and finite element method","authors":"Junming Hou,&nbsp;Jiuyu Jin,&nbsp;Zhi Ma,&nbsp;Hao Ding,&nbsp;Chenglong Li,&nbsp;Minghui Liu,&nbsp;Qiang Tang,&nbsp;Chenghao Li","doi":"10.1016/j.jspr.2025.102652","DOIUrl":"10.1016/j.jspr.2025.102652","url":null,"abstract":"<div><div>Cyperus esculentus L is a newly emerging multi-purpose food, which is also a raw material for drinks or brewing. It is susceptible to mechanical damage during mechanical harvesting, stored and food processing. The compression bruise is an important factor that affects the quality of Cyperus esculentus L. The compression bruise's region will be intruded into by bacteria, resulting in a reduction of food quality. Therefore, it is significant to study the compression bruise mechanism. Its mechanical properties are measured by experiment and applied as a reference to simulation. The macroscopic characteristics of the bruise were observed, and the bruised area was extracted. The experiments were carried out, which the loading displacement, loading speed and moisture content were selected as the factors. It is found that when the loading speed is 20 mm/min, the moisture content is 38 % and the loading displacement is 1.37 mm, the minimum loading force is 94.704 N and the minimum bruised area ratio is 18.924 %. The 3D scanning method was applied to establish the model of Cyperus esculentus L. The results of simulation show when the equivalent strain threshold is 0.10, the relative error is 15.05 % compared with experiment. The changes of equivalent stress, equivalent strain and internal energy are analyzed in the process of compression. The bruised volume and bruised sensitivity are analyzed in the condition of different loading displacements. The bruised sensitivity increases from 8955.24 mm³/mJ to 16814.82 mm³/mJ, when the loading displacement changes from 0.5 mm to 2.0 mm. The bruised sensitivity reduced from 16814.82 mm³/mJ to 13725.42 mm³/mJ, when the loading displacement from 2.0 mm to 2.5 mm.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102652"},"PeriodicalIF":2.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of α-glucosidases as potential targets for the control of corn weevil, Sitophilus zeamais (Coleoptera, Curculionidae)","authors":"Essicka Andrea García-Saldaña,&nbsp;Abraham Vidal-Limon,&nbsp;Esaú Bojórquez-Velázquez,&nbsp;Eliel Ruíz-May,&nbsp;José Miguel Elizalde-Contreras,&nbsp;Luis Arturo Ibarra-Juárez,&nbsp;José Antonio Guerrero-Analco,&nbsp;Felipe Barrera-Méndez,&nbsp;Jesús Alejandro Zamora-Briseño","doi":"10.1016/j.jspr.2025.102650","DOIUrl":"10.1016/j.jspr.2025.102650","url":null,"abstract":"<div><div>The maize weevil, <em>Sitophilus zeamais</em>, is one of the most relevant insect pests of stored corn grains. There are currently few alternatives for its control, most of which are synthetic pesticides. In this work, we explored the potential to control <em>S. zeamais</em> by targeting its α-glucosidases. By using different approaches, we successfully identified two non-redundant α-glucosidases. <em>In silico</em> analysis revealed that both proteins had conserved structural motifs related to α-glucosidases. Molecular dynamics simulation indicated that these enzymes are able to interact with the α-glucosidase inhibitor, acarbose. The acarbose-α-glucosidase complexes exhibited a high affinity between the inhibitor and the catalytic site. Feeding adult <em>S. zeamais</em> with acarbose-impregnated maize resulted in over 30 % mortality, suggesting that the application of α-glucosidase inhibitors could enhance the resistance of maize grains against <em>S. zeamais</em>.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102650"},"PeriodicalIF":2.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143868405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal factors influence the organoleptic quality and shelf-life of fresh-cut turnip greens","authors":"Karin Albornoz,&nbsp;Zenaba Abdissa","doi":"10.1016/j.jspr.2025.102661","DOIUrl":"10.1016/j.jspr.2025.102661","url":null,"abstract":"<div><div>Turnip greens are leafy vegetables from the <em>Brassica</em> family highly popular in the Southeast United States, where they are sold in whole and fresh-cut formats. Fresh-cut processing favors convenience but accelerates leaf senescence and quality degradation, contributing to consumer rejection and postharvest losses and waste. Turnip greens, cultivated year-round in different regions, are exposed to variable growing conditions across seasons. While seasonal variations in nutritional and organoleptic quality are documented for other species during postharvest storage, this has not been investigated in turnip greens in fresh-cut format. In this study, turnip greens grown in two seasons—fall and winter-spring—were fresh-cut processed and stored at 5 °C for up to 21 days. Subjective (marketability scores), and objective (color, total chlorophyll, carotenoids, polyphenols and ammonia contents) parameters were monitored at 0, 7, 14, and 21 days of storage. Results showed that while sensory quality declined during storage in both seasons, leaves grown in the fall exhibited a significantly faster deterioration rate compared to winter-spring samples. This trend mirrored the higher rates of chlorophyll and fresh weight loss shown by turnip greens grown in the fall. Seasonal variations in environmental factors and agronomic practices likely induced distinct physiological responses, influencing postharvest quality and shelf-life. This is the first report of organoleptic and compositional quality changes in fresh-cut turnip greens affected by the growing season. Tailoring postharvest practices to seasonal shelf-life variations can contribute to minimizing food losses and optimizing produce supply chain performance.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102661"},"PeriodicalIF":2.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultures of stored-product mite Tyrophagus putrescentiae experimentally infected with Cardinium and Wolbachia presented reduced fitness","authors":"Jan Hubert ,&nbsp;Eliza Glowska-Patyniak ,&nbsp;Stano Pekar","doi":"10.1016/j.jspr.2025.102663","DOIUrl":"10.1016/j.jspr.2025.102663","url":null,"abstract":"<div><div><em>Tyrophagus putrescentiae</em> is a cosmopolitan pest of stored food and animal feed. Mite populations differ in their microbiome composition, resulting in variability in their fitness. Cultures of the stored-product mite <em>T. putrescentiae</em> are often singly infected by one of intracellular bacterial genera <em>Cardinium</em> and <em>Wolbachia</em>. No naturally occurring multi-infected (<em>Cardinium/Wolbachia</em>) cultures have been observed.</div><div>Under laboratory conditions, we mixed two singly infected mite cultures, i.e., <em>Cardinium</em>-infected (5L and 5S) and <em>Wolbachia</em>-infected (5N and 5P) cultures, to obtain four experimental cultures (5LN, 5LP, 5SN and 5SP), each infected with each of the endosymbionts. Population growth was used as a fitness indicator, and PCR with specific primers for a single mite was used to identify the prevalence of <em>Cardinium</em> and <em>Wolbachia</em> in the parental and mixed cultures.</div><div>The population growth of the experimental cultures was lower than that of the parents at the beginning of the experiment. After six months, 5SN and 5SP exhibited greater fitness than 5LP and 5LN. The population growth of the 5SN and 5SP cultures did not differ from that of the parental cultures 5P and 5S. <em>Cardinium</em>-infected mites prevailed in 6 cultures, <em>Wolbachia</em>-infected mites in 5 cultures, and asymbiotic individuals in 1 culture after six months. The proportion of <em>Cardinium-</em>infected individuals (45 %) was greater than that of <em>Wolbachia</em>-infected individuals (25 %), indicating that <em>Cardinium</em>-infected mites had a fitness advantage over their <em>Wolbachia</em>-infected counterparts. Although the cultures were multi-infected, doubly infected mites were rare (7 %).</div><div>These results suggest that <em>Cardinium</em> is responsible for cytoplasmic incompatibility in <em>T. putrescentiae</em>. The results revealed that the presence of the intracellular symbionts <em>Cardinium</em> and <em>Wolbachia</em> strongly influenced mite population growth in the experiments. These data support the importance of the microbiome in this pest.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"112 ","pages":"Article 102663"},"PeriodicalIF":2.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}