Journal of Stored Products Research最新文献

{"title":"Harnessing artificial intelligence and machine learning for fruit quality management: A comprehensive review","authors":"Hossam S. El-Beltagi ,&nbsp;Mohamed Gad ,&nbsp;Nagwa Khedr ,&nbsp;Mohamed Abdel-Haleem ,&nbsp;Mohamed S. Al Saikhan ,&nbsp;Tarek A. Shalaby ,&nbsp;Mohamed M. El-Mogy ,&nbsp;Emad H. Khedr","doi":"10.1016/j.jspr.2025.102920","DOIUrl":"10.1016/j.jspr.2025.102920","url":null,"abstract":"<div><div>Plant-derived foods are essential components of the human diet, and their postharvest quality profoundly affects market value, storage stability, and consumer acceptance. Conventional quality evaluation methods relying on manual inspection are often time-consuming, subjective, and inconsistent, creating an urgent need for intelligent, automated alternatives. This review provides a comprehensive synthesis of recent advances in artificial intelligence (AI) and machine learning (ML) for non-destructive fruit quality assessment. Emphasis is placed on imaging-based technologies including RGB, multispectral, hyperspectral, X-ray, and magnetic resonance imaging that enable precise extraction of external and internal quality attributes such as color, texture, geometry, and biochemical composition. The integration of AI with complementary sensing systems, such as electronic noses and tongues, Internet of Things networks, and robotic platforms, is also discussed for achieving real-time sorting, grading, and early disorder detection. Key insights reveal the growing efficacy and versatility of AI-driven solutions in enhancing precision, speed, and sustainability of postharvest management. Nevertheless, challenges remain regarding dataset standardization, model interpretability, and cross-commodity generalization. Future research should focus on explainable AI, multimodal data fusion, and large-scale validation under practical conditions. Collectively, AI-enabled technologies are redefining postharvest fruit quality management toward a more intelligent and sustainable future.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102920"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of host switching on population rejuvenation and transgenerational fitness in the parasitoid wasp Habrobracon hebetor","authors":"Yao Huang ,&nbsp;Jintao Wen ,&nbsp;Chunlin Tian ,&nbsp;Ang Li ,&nbsp;Xu Yang ,&nbsp;Guowei Li ,&nbsp;Siqing Jiang ,&nbsp;Yi Mao ,&nbsp;Maofa Yang","doi":"10.1016/j.jspr.2025.102915","DOIUrl":"10.1016/j.jspr.2025.102915","url":null,"abstract":"<div><div>Host switching, a strategy for population rejuvenation, is essential for sustaining pest control efficacy of parasitic natural enemies. This study evaluated the effects of switching the host of <em>Habrobracon hebetor</em>, reared long-term on <em>Ephestia elutella</em>, to either <em>Plodia interpunctella</em> or <em>Corcyra cephalonica</em> for five consecutive generations. The assessment focused on the parasitoid's subsequent parasitism fitness toward the original host, <em>E. elutella</em>, as well as the across parental and first offspring generations effects. Results showed that host switching significantly increased the number of hosts paralyzed, number of hosts parasitized, and fecundity of the parental generation of <em>H. hebetor</em> on <em>E. elutella</em>. The <em>P. interpunctella</em>–switched group achieved the highest number of parasitized hosts and fecundity, along with significantly extended female longevity. Offspring experiments revealed a shorter larval developmental period in the <em>P. interpunctella–</em>switched population with high percentages of pupal formation and adult emergence. Offspring from this group also showed significantly superior paralysis, parasitism, and fecundity relative to the control and <em>C. cephalonica–</em>switched groups, indicating that fitness benefits from host-switching persist across generations. Overall, <em>P. interpunctella</em> emerged as an optimal alternate host for rejuvenating <em>H. hebetor</em> populations, enhancing parasitic performance and longevity, likely through nutritional or physiological mechanisms. This study provides theoretical and practical guidance for rejuvenating <em>H. hebetor</em> in mass rearing under laboratory conditions, with application value for improving biological control efficacy.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102915"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-guided infrared drying of spearmint: Energy-efficient fixed vs. two-step temperature methods for phytochemical retention and market-compliant quality","authors":"Amin Hazervazifeh ,&nbsp;Parviz A. Moghaddam ,&nbsp;Farzad Pashmforoush ,&nbsp;Hamid Hatami Maleki","doi":"10.1016/j.jspr.2026.102953","DOIUrl":"10.1016/j.jspr.2026.102953","url":null,"abstract":"<div><div>This study investigates the performance of an infrared drying system using fixed (FTM) and two-step temperature methods (TTM) in preserving the phytochemical and color attributes of spearmint leaves, alongside an energy analysis and the development of a machine learning (ML)-based predictive model. Results showed that color degradation as a quality parameter intensified with rising temperature, peaking at 72 °C (ΔE = 16.68) under FTM, whereas TTM, which applies a strategic 10 °C temperature drop upon reaching the falling rate period, significantly mitigated discoloration, e.g., reducing ΔE from 16.36 (70 °C) to 13.69 (70_to60°C). GC analysis revealed that 48_to38°C of TTM yielded the highest concentrations of key phytochemical constituents, while 68 °C of FTM demonstrated superior retention among fixed-temperature treatments. Principal component analysis (PCA) discriminated effectively between drying protocols, with the first two components explaining 72 % of the variance and highlighting distinct phytochemical and color degradation patterns. TTM consumes 4.98–8.45 % less energy than the corresponding fixed process at the second-step temperature (58_to48°C vs. 48 °C; 72_to62°C vs. 62 °C, respectively) and reduces color degradation by 9.5–16.5 % compared to the fixed process at the first-step temperature (72_to62°C vs. 72 °C and 58_to48°C vs. 58 °C), establishing a dual-reference benchmark. Three ML algorithms, Least Squares Boosting (LSBoost), Random Forest (RF), and Support Vector Machine (SVM), were employed to develop predictive models for key phytochemicals. LSBoost provided the highest predictive accuracy for 1,8-Cineole and Pulegone, achieving R² values of 97 % and 96 %, respectively, whereas RF yielded the most accurate predictions for Menthone with an R² of 98 %. In terms of computational performance, RF exhibited the lowest CPU time and highest overall efficiency, followed by LSBoost and SVM. Bayesian-optimized hyperparameters enhanced model generalization, validated via k-fold cross-validation and independent testing (R²: 0.88–0.90, RMSE: 0.39–1.84).</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102953"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal effect of nitrogen dioxide fumigation on Aspergillus flavus in artificially infected unshelled peanuts","authors":"Sookyung Oh ,&nbsp;Rippy Singh ,&nbsp;Yong-Biao Liu","doi":"10.1016/j.jspr.2026.102956","DOIUrl":"10.1016/j.jspr.2026.102956","url":null,"abstract":"<div><div>This study investigated the fungicidal efficacy of nitrogen dioxide (NO₂) fumigation against <em>Aspergillus flavus</em> on unshelled peanuts. Artificially infected peanuts were fumigated with NO₂ at doses of 0.0 % (control), 0.1 %, 0.3 %, and 1.0 % for 1, 2, and 3 days. Quantitative analysis using the GreenLight™ enumeration test showed a dose-dependent inhibitory effect. For instance, after one day, intact peanuts treated with 0.1 %, 0.3 %, and 1.0 % NO₂ showed Log CFU/mL values of 1.11, 0.24, and 0.00, respectively, compared to the control's 3.17 Log CFU/mL. Molecular corroboration through PCR showed reduced amplifiable <em>A. flavus</em>-specific DNA with complete inactivation at 1.0 % NO₂. A key observation was the differential efficacy over extended durations. After three days, 0.1 % NO₂ significantly reduced surface <em>A. flavus</em> but failed to yield a significant reduction for internal populations in cracked peanuts (2.38 Log CFU/mL vs. 3.05 Log CFU/mL in control), highlighting the protective barrier of the peanut shell. This research underscored the potential of NO₂ fumigation for mitigating aflatoxin risk. Future studies should evaluate efficacy against other foodborne pathogens, assess safety regulations, quality attributes, and residue levels for commercial use.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102956"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An efficient chitinase from Bacillus amyloliquefaciens M6: Immobilization to ZnO/RGO nanocomposite and its application in biocontrol","authors":"Sevda Uçar ,&nbsp;Neslihan Dikbaş ,&nbsp;Şeyma Alım ,&nbsp;Emir Çepni ,&nbsp;Göksel Tozlu ,&nbsp;Merve Şenol Kotan ,&nbsp;Tuba Öznülüer Özer ,&nbsp;Muhammed Tatar ,&nbsp;Kağan Kökten ,&nbsp;Tolga Karaköy ,&nbsp;Barış Binay","doi":"10.1016/j.jspr.2026.102958","DOIUrl":"10.1016/j.jspr.2026.102958","url":null,"abstract":"<div><div>In this study, the chitinase enzyme purified from <em>Bacillus amyloliquefaciens</em> M6 was immobilized on Zinc oxide/reduced graphene oxide nanocomposites (ZnO/RGO NCs), and its activity on <em>Rhyzopertha dominica</em> and <em>Oryzaephilus surinamensis</em> was tested. To optimize the culture medium, the M6 strain was developed under varying conditions, and the optimum substrate concentration, temperature, pH, and incubation time for chitinase production were determined as 5 g/L chitin, 35 °C, 6.0 pH, and 24 h. Compared to its activity prior to optimization (10.42 U/mL), the chitinase activity of the strain was observed to increase by 2.2 times. Under these conditions, chitinase was purified 6.75-fold from strain M6 with a specific activity of 228.90 U/mg (activity: 11,48 U/mL) and a yield of 1.84 %, and its molecular weight was determined to be ∼50 kDa. Chitinase was immobilized on ZnO/RGO NCs with 99.68 % immobilization efficiency and 94.30 % recovered activity. The optimum pH and temperature values of free and immobilized chitinase were determined as 6.0 and 45 °C. The activity of immobilized chitinase against <em>R. dominica</em> and <em>O. surinamensis</em> was tested and the LC₅₀ and LC₉₀ values for <em>R. dominica</em> were calculated as 1.8 mg/mL (32.8 U/mL) and 3.8 mg/mL (69.2 U/mL) and 4.8 mg/mL (87.4 U/mL) and 10.0 mg/mL (182.0 U/mL) for <em>O. surinamensis</em>. Thus, the obtained results concluded that the immobilized chitinase proved an alternative biocontrol agent to hazardous synthetic pesticides, the application of this biocontrol agent to make biopesticides will prove more reliable for sustaining the crop productivity and in crop protection.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102958"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing maize grain cleaning before storage using a multi-dovetail shape and planar combined sieve: CFD-DEM simulation and experimental validation","authors":"Zhilei Yang ,&nbsp;Lijun Wang ,&nbsp;Yanyu Zhang ,&nbsp;Shuheng Wang ,&nbsp;Wenyao Huang ,&nbsp;Lixiang Liao","doi":"10.1016/j.jspr.2026.102951","DOIUrl":"10.1016/j.jspr.2026.102951","url":null,"abstract":"<div><div>Grain cleaning is a critical process for ensuring storage safety and reducing postharvest losses, primarily by removing impurities such as stalks and cobs and improving grain quality. However, uneven transverse dispersion of particles on the sieve surface often resulted in material accumulation and reduced cleaning efficiency. To address this issue, a multi-dovetail shape and planar combined sieve (M-DSPCS) was designed to promote rapid transverse dispersion of particles on the sieve surface. The dispersion mechanism of the dovetail-shaped units was analyzed using Computational Fluid Dynamics and the Discrete Element Method (CFD-DEM), and the simulation results were validated through physical experiments. The results show that the transverse dispersion of particles is accelerated by the dovetail-shaped unit structure, thereby improving the transverse dispersion uniformity of particles on the sieve. Simultaneously, the effective screening area of the sieve was increased and the contact rate between particles and the sieve surface (CRPSS) was enhanced, which increased the probability of maize grains passing through the sieve and thereby reduced maize grain losses. Under a feeding rate of 7 kg s⁻¹, the M-DSPCS achieved a cleaning rate and loss rate of 99.03 % and 0.81 %, respectively. Compared with the planar sieve, cleaning rate increased by 1.87 %, adding 123.1 kg of effective maize grains per hectare, while loss rate decreased by 1.40 %, saving 92.3 kg of maize grains per hectare. This study proposed an effective approach for improving maize grain cleaning quality prior to storage and provided scientific guidance for the design and optimization of grain cleaning devices.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102951"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance grade of different Triticum species toward Sitophilus oryzae L. (Coleoptera: Curculionidae)","authors":"Salvatore Guarino ,&nbsp;Shaun Gasparro ,&nbsp;Giuliano Cerasa ,&nbsp;Antonioni Acacio Campos Moliterno ,&nbsp;Rosolino Ingraffia ,&nbsp;Antonella Lo Porto ,&nbsp;Giacomo Gargano ,&nbsp;Paolo Ruisi ,&nbsp;Dario Giambalvo ,&nbsp;Ezio Peri","doi":"10.1016/j.jspr.2025.102892","DOIUrl":"10.1016/j.jspr.2025.102892","url":null,"abstract":"<div><div>Varietal resistance in cereals represents a promising and sustainable strategy for managing biotic stressors, including insect pests during storage. In this study, eighteen genotypes from six cereal species - <em>Triticum monococcum</em>, <em>T. dicoccum</em>, <em>T. spelta</em>, <em>T. durum</em>, <em>T. aestivum</em>, and <em>Triticosecale</em> - were evaluated for their resistance to the rice weevil, <em>Sitophilus oryzae</em> L., one of the most destructive stored product pests. The objective was to assess differences in susceptibility by measuring some insect parameters, such as adult mortality, F1 progeny production and body size, along with grain traits such as kernel hardness and weight. For this scope, bioassays included both non-choice and two-way choice tests to evaluate insect performance and preference. Significant inter- and intra-specific variation in susceptibility was observed. In non-choice bioassays, <em>Sitophilus oryzae</em> generally showed higher adult mortality and lower adult progeny emergence feeding and ovipositing on <em>T. durum</em> and <em>T. dicoccum</em> genotypes, suggesting greater resistance of these genotypes; on the contrary <em>T. monococcum</em> and triticale appear more susceptible. Grain hardness was strongly and negatively correlated with adult progeny output, supporting its role as a mechanical barrier to larval development. However, kernel hardness did not influence adult body size, suggesting that individuals overcoming this barrier can still complete normal development. Kernel weight showed a positive correlation with female size, indicating a potential fitness advantage for the insects feeding on larger seeds. In two-choice assays, <em>S. oryzae</em> showed a preference for softer genotypes, particularly triticale Agostino and Tribeca, over harder genotypes such as durum wheat Perciasacchi and Aureo. These results provide new insights into the resistance potential of traditional and modern <em>Triticum</em> genotypes. The integration of resistant genotypes, especially from <em>T. durum</em> and <em>T. dicoccum</em>, into breeding programs and storage systems may contribute to reducing chemical inputs and improving long-term grain protection strategies.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102892"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptides from Leptogenys processionalis exhibit Antimicrobial activity against key pathogens of postharvest pomegranate (Punica granatum L.)","authors":"B.N. Ramesh ,&nbsp;C.V. Babu ,&nbsp;S.D. Pradeep ,&nbsp;E. Dilip ,&nbsp;P.A. Niveditha","doi":"10.1016/j.jspr.2025.102894","DOIUrl":"10.1016/j.jspr.2025.102894","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs) are eco-friendly alternatives to chemical pesticides due to their broad-spectrum activity and reduced risk of resistance. Their role in postharvest disease control of fruits such as pomegranate (<em>Punica granatum</em> L.) is still largely unexplored. In this study, crude AMPs from the predatory ant <em>Leptogenys processionalis</em> were evaluated for antibacterial and antifungal activity. The crude extract showed strong antibacterial effects against <em>Escherichia coli</em>, <em>Staphylococcus aureus</em>, and <em>Xanthomonas</em> spp., with inhibition zones up to 50 ± 1.22 mm² in diameter at 70 μg/disk, and concentration-dependent antifungal activity against <em>Alternaria alternata</em>, reaching 94 ± 0.07 % inhibition at 500 μg/mL. RP-HPLC fractionation yielded three fractions, of which Fraction 1 had the highest protein concentration (2.09 mg/mL) and displayed maximum activity, producing inhibition zones up to 84.72 ± 1.60 mm² in diameter at 300 μg/disk and 91.07 ± 0.37 % inhibition of <em>Alternaria. alternata</em> at 500 μg/mL. MALDI-TOF MS analysis revealed prominent peptide peaks, including one at 1794.13 m/z, probably responsible for antimicrobial effects. Importantly, fruit assays demonstrated that crude extract at 300 μg/mL achieved 98 ± 0.4 % inhibition, statistically equivalent to commercial fungicide treatment (97 ± 0.5 %). These results identify <em>L. processionalis</em> as a novel AMP source with strong potential for sustainable postharvest disease management in pomegranate.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102894"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145623247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphane exposure levels during good practice fumigation using metal phosphide formulations: A simulation study for post-harvest treatments","authors":"Daniel Vetter ,&nbsp;Katharina Maria Lippert ,&nbsp;Marcel René Schumann ,&nbsp;André Schäfer ,&nbsp;Jutta Schade ,&nbsp;Marie-Carolin Goetze","doi":"10.1016/j.jspr.2025.102918","DOIUrl":"10.1016/j.jspr.2025.102918","url":null,"abstract":"<div><div>Fumigation products containing magnesium phosphide (Mg₃P₂) are widely used in agricultural and storage pest control. Magnesium phosphide fumigants offer several compelling advantages including efficacy against all developmental stages of insects, decomposition into non-toxic residues, quick and easy application when used as pre-packed formulation. Because the active ingredient is phosphane (PH₃) that is released from the fumigant product when exposed to moisture (e.g., as air humidity) such fumigants require careful handling by trained professionals to ensure human safety. In this study, occupational exposure levels to PH₃ during the use of magnesium phosphide plates were monitored. For this purpose, the plates were applied following good practice procedures in two stationary treatment objects (37 m³ in volume each) and exposure levels were continuously monitored until final gas clearance. A total of six trials each lasting for four consecutive days were conducted. 39 samplers generating more than 7 million data points were used, and the results are presented in this paper. The monitoring results clearly indicate that there is no undue risk for operators in the scenarios investigated using products containing Mg₃P₂ when applying good practice.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102918"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145748792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tribolium castaneum CO2 controlled atmosphere stress screening and functional verification of CYP genes","authors":"Yanfei Zhou,&nbsp;Min Zhou,&nbsp;Liwen Guan,&nbsp;Yi Zhang,&nbsp;Yuhang Xie,&nbsp;Xinyu Zhang,&nbsp;Shangrong Hu,&nbsp;Yuya Zhang,&nbsp;Bin Tang","doi":"10.1016/j.jspr.2025.102913","DOIUrl":"10.1016/j.jspr.2025.102913","url":null,"abstract":"<div><div>Cytochrome P450 (CYP450) possesses a potent detoxification ability, allowing insects to withstand both the defensive compounds of plants and the toxicity of pesticides. The objective of this study was to investigate the physiological and biochemical responses, as well as the underlying molecular mechanisms of the CYP4/9 family genes in <em>Tribolium castaneum</em> under high carbon dioxide stress. Based on omics analysis, six CYP genes related to trehalose and responsive to CO₂ stress were selected. RNA interference was used for functional validation. The results revealed that dsCYP treatment affected trehalose metabolism in <em>T. castaneum</em>, leading to phenotypic changes like reduced body length and weight loss. CYP450 superfamily members, such as <em>TcCYP4BN1</em>, <em>TcCYP9F2</em>, and <em>TcCYP9AB1</em>, played crucial roles in <em>T. castaneum</em>'s adaptation to high CO₂ stress, affecting beetle growth indicators. These results provide new insights for the further development and improvement of controlled atmosphere storage technology and effective control of storage pests.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"116 ","pages":"Article 102913"},"PeriodicalIF":2.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145748794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}