Journal of Stored Products Research最新文献

{"title":"Effects of modified atmospheres, temperature and humidity on the growth and development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) in feihe infant milk powder","authors":"","doi":"10.1016/j.jspr.2024.102378","DOIUrl":"10.1016/j.jspr.2024.102378","url":null,"abstract":"<div><p><em>Tribolium castaneum</em> (Herbst) is a common stored product pest that damages various stored goods across the global<em>.</em> To identify the effects of modified atmospheres (MA), humidity and temperature on the growth and development of <em>T. castaneum</em> in Feihe infant milk powder<em>,</em> the modified atmospheres were set at 75% nitrogen (N₂) and 25% carbon dioxide (CO₂), with air as the control. Two different temperatures and humidities were set at 30 °C, RH 80% and 25 °C, RH 40%. The results revealed that <em>T. castaneum</em> larvae exposed to the warm and moist southern conditions (30 °C, RH 80%) feeding on flour and yeast diet had a significantly shorter developmental duration. The larval weights were significantly increased in <em>T. castaneum</em> feeding flour and yeast diet compared with those of <em>T. castaneum</em> feeding milk powder under high temperature. The elevated temperature significantly increased the mortality of larvae. However, 4.4% of the survival larvae successfully grow into adults at the cool and dry northern conditions (25 °C, RH 40%). The mortality rate of eggs was 100% when eggs were sealed and treated by MA under warm and moist temperature and humidity for 96 h, and the mortality rate of 5th instar larvae increased to 100% on 7th day. Moreover, the activities of CAT and GST were decreased in all stages of <em>T. castaneum</em> feeding on infant milk under temperature and humidity stress. Additionally, 5th instar larvae fed with infant milk powder showed significantly fluctuated activity of POD, SOD and P450 in all stages of <em>T. castaneum</em>. However, <em>T. castaneum</em> is more susceptible to milk powder sealing with air under warm and moist temperature and humidity. The present investigation demonstrated that temperature treatment and modified atmospheres effectively prevented growth and development of <em>T. castaneum</em> in milk powder. These results also contribute to suitable strategies for storage of milk power.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141622806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel foodborne source of Xerochrysium xerophilum (Pitt) Pitt, raw peanuts stored at low moisture and high CO2","authors":"Renee S. Arias,&nbsp;Valerie A. Orner,&nbsp;Victor S. Sobolev,&nbsp;Alicia N. Massa,&nbsp;Paola C. Faustinelli,&nbsp;Travis E. Walk,&nbsp;Marshall C. Lamb,&nbsp;Christopher L. Butts","doi":"10.1016/j.jspr.2024.102381","DOIUrl":"https://doi.org/10.1016/j.jspr.2024.102381","url":null,"abstract":"<div><p>Peanut seed is ideally stored at low water activity (a_w) and low temperature, since common mycotoxigenic fungi cannot survive below 0.8 a_w, and the life cycle of seed-infesting insects is interrupted below 15 °C. Hermetic storage bags at 0.77 a_w, room temperature, and ≥ 9000 mg/m³ CO₂, were tested for their potential use in countries where temperature control is unaffordable. Despite these inhospitable conditions, peanuts displayed an unusual white powder that we identified as the xerophilic fungus <em>Xerochrysium xerophilum</em> (syn. <em>Chrysosporium xerophilum</em>), and sequenced its genome. Previously, only 19 sequences shorter than 1700 nt were available. For six decades, only three food sources of <em>X</em>. <em>xerophilum</em> were known: dry prunes, chocolate, and dry coconut. Here we report peanut as a novel food source of this extremophile and make one isolate available at the USDA culture collection. Given the slow growth of this fungus in culture, we developed a robust PCR to sequence and identify <em>X. xerophilum</em> on contaminated peanut seed without performing DNA extraction. The broad range of oxygen, a_w and temperatures that support growth of <em>X. xerophilum</em> will significantly affect the decisions made regarding the conditions to safely store peanuts.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022474X24001383/pdfft?md5=99c4276133c0dc812d35476af352295e&pid=1-s2.0-S0022474X24001383-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan/mandarin essential oil-based films on citrus fruits for the control of the medfly attack and to prevent the occurrence of grey and blue mould in post-harvest","authors":"Prangthip Parichanon ,&nbsp;Priscilla Farina ,&nbsp;Isabel Vicente ,&nbsp;Marco Cesarini ,&nbsp;Eliverta Hotaj ,&nbsp;Sabrina Sarrocco ,&nbsp;Elisa Pellegrini ,&nbsp;Barbara Conti","doi":"10.1016/j.jspr.2024.102380","DOIUrl":"https://doi.org/10.1016/j.jspr.2024.102380","url":null,"abstract":"<div><p>Citrus fruits, widely consumed around the world, can be negatively affected by pests and fungal infections during their cultivation, handling, transportation, and storage, thus resulting in substantial yield losses and food waste. The use of natural preservatives like chitosan (CHT) and essential oils (EOs) is a promising approach for reducing chemical inputs to preserve food products.</p><p>This study investigated the effects of CHT, extracted from crab shells and the fungus <em>Pleorotus ostreatus</em>, alone and in combination with mandarin (<em>Citrus</em> × <em>reticulata</em> Blanco, Rutaceae) essential oil (MEO), as an oviposition deterrent towards <em>Ceratitis capitata</em> (Diptera: Tephritidae), the Mediterranean fruit fly, and growth inhibitor of <em>Penicillium</em> (<em>P. expansum</em>, <em>P. digitatum,</em> and <em>P. italicum</em>) spp. fungi, the causal agents of apple and citrus rot.</p><p>A solution of 1.0% CHT of both origins (from crab shells and <em>P. ostreatus</em>) added with MEO resulted as the best combination to significantly reduce the oviposition percentage of <em>C. capitata</em> as well as mycelial growth and spore germination of <em>Penicillium</em> isolates and their pathogenic activity on <em>Citrus japonica</em> Thunb. (kumquats) fruits.</p><p>According to results here collected, CHT added with MEO represents a valid combination to be used as an edible film and coating as part of an integrated control strategy to improve the shelf-life of fresh citrus fruits. Furthermore, fungal CHT, here used for the first time in combination with MEO, can be an excellent alternative to reply to the eating habits and necessities of the final consumers.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-dependent development of Carpophilus marginellus Motschulsky, 1858 (Coleoptera: Nitidulidae) and its larval morphological characteristics","authors":"Shipeng Shao ,&nbsp;Gengwang Hu ,&nbsp;Xin Tang,&nbsp;Liangliang Li,&nbsp;Yinghui Wang,&nbsp;Yi Guo,&nbsp;Chengtao Kang,&nbsp;Yundi Gao,&nbsp;Ruonan Zhang,&nbsp;Siqi Liu,&nbsp;Zhiya Gu,&nbsp;Yu Wang","doi":"10.1016/j.jspr.2024.102382","DOIUrl":"https://doi.org/10.1016/j.jspr.2024.102382","url":null,"abstract":"<div><p><em>Carpophilus marginellus</em> Motschulsky is a storage pest that damages fruits, dried fruits, grains, and other agricultural produce. Because the beetle is also saprophagous and has been found on corpses, it is of forensic interest. Despite its agricultural and forensic importance, biological research on this species and this family is lacking. Most damage is caused by the larval stage, but there are no morphological studies on the larvae of this species. Herein, we photographed the three instars using optical and scanning electron microscopy (SEM) to visualize their morphology more vividly. We also described the structure of the different parts and compared the morphological differences between the different instars for accurate identification. In addition, we reared <em>C. marginellus</em> at seven constant temperatures between 16 and 34 °C, recorded the developmental duration and accumulated degree days (ADD) of each developmental stage, measured the body length, head capsule width, and the distance between the urogomphi at different times. The mortality rate at each stage was also calculated. Based on the above data, we plotted an isomorphen diagram, isomegalen diagram, and survival curve, established linear thermal summation models and curvilinear Optim SSI models, and performed a cluster analysis of the head capsule width and the distance between the urogomphi. The morphological and developmental information of <em>C. marginellus</em> provided in this study is useful for identifying the species, mass rearing, pest management, and forensic postmortem interval (PMI) estimation.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of edible coatings as a new sustainable alternative to extend the shelf life of strawberries (Fragaria ananassa): A review","authors":"Thamyres César de Albuquerque Sousa,&nbsp;Igor Henrique de Lima Costa,&nbsp;Eliezer Avila Gandra,&nbsp;Adriana Dillenburg Meinhart","doi":"10.1016/j.jspr.2024.102375","DOIUrl":"https://doi.org/10.1016/j.jspr.2024.102375","url":null,"abstract":"<div><p>Strawberry (<em>Fragaria ananassa</em>), a widely sold fruit across the globe, saw an estimated harvest of a staggering 9.57 million tons in 2022 (<span>FAO, 2022</span>). Strawberries have a limited shelf life and senescence period due to their high respiration rate and perishability. Mechanical damage and infections caused by various pathogens, including fungi, further contribute to color, texture, quality, and post-harvest losses. In search of solutions to preserve their physical, microbiological, and sensorial qualities, several studies have explored the use of edible coatings, a practice widely used in the industry to preserve fruits and vegetables. Chitosan remains the most widely used material for strawberry coatings despite the growing exploration of novel polymeric materials, as evidenced by bibliometric analysis. This review delves into the most prevalent coatings employed to preserve the quality attributes of strawberries and extend their shelf life. Data quality is rigorously maintained through a multivariate analysis based on bibliometrics of metadata extracted from research studies in the Web of Science, utilizing the keywords \"strawberry\" and \"coatings\" as the search strategy.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141542743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in cottonseed meal quality during post-harvest processing of cottonseed","authors":"Catelyn Bridges,&nbsp;Robert Hardin,&nbsp;Janie McClurkin-Moore","doi":"10.1016/j.jspr.2024.102371","DOIUrl":"https://doi.org/10.1016/j.jspr.2024.102371","url":null,"abstract":"<div><p>This study investigates aflatoxin contamination levels during the post-harvest processing of whole cottonseed into defatted cottonseed meal, a critical feed component in the dairy industry. Whole fuzzy cottonseed, a byproduct of cotton gins, undergoes various processes to extract oil and produce meal and hulls. These are either used as animal feed or soil amendments. However, aflatoxin contamination poses significant challenges due to strict FDA action levels for dairy and beef cattle feed. This contamination occurs when <em>Aspergillus flavus</em> infects crops under specific environmental conditions or through improper handling during post-harvest processes. Our research aims to document aflatoxin levels during each stage of post-harvest processing, from cleaning to meal formation. Preliminary findings suggest that certain stages may concentrate aflatoxins akin to other agricultural products like dried distillers’ grains and solubles (DDGS). The implications of these findings could be profound for the dairy industry, where low allowable aflatoxin levels impact both economic and animal health outcomes. The cottonseed post-harvest process consists of cleaning, delinting, hull removal, kernel flaking, oil extraction, and meal formation. Understanding how aflatoxin levels change during these steps is crucial for ensuring safe and high-quality defatted cottonseed meal for livestock consumption. Microbial levels increased by 4 orders of magnitude during the acid delinting process, from 7.0× 10⁵ to 5.2 × 10⁹ CFU/g, and 5 orders of magnitude during the mechanical delinting process, from 7.3× 10⁵ to 1.5 × 10¹⁰ CFU/g for the inoculated cottonseed samples. Higher microbial load was associated with lower protein levels and lower fat acidity levels. Further research will shed light on the effects of aflatoxin concentration on the nutritional value of the final product.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141481545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential preference of grain of landrace and commercial rice genotypes to Sitophilus oryzae (L.) (Coleoptera: Curculionidae) attack","authors":"Kamal Ravi Sharma ,&nbsp;S.V.S. Raju ,&nbsp;Sameer Kumar Singh ,&nbsp;Rashmirekha Singh ,&nbsp;Rajendran Dhanapal ,&nbsp;Rakesh Kumar","doi":"10.1016/j.jspr.2024.102373","DOIUrl":"https://doi.org/10.1016/j.jspr.2024.102373","url":null,"abstract":"<div><p>The rice weevil, <em>Sitophilus oryzae</em> L. (Coleoptera: Curculionidae), is one of the most damaging pests to stored cereal grains worldwide. The current study aimed to clarify the relationship between <em>S. oryzae</em> development and the governing physicochemical grain properties. Laboratory experiments were conducted to determine the grain preference of various landrace and commercial rice genotypes to rice weevil. <em>S. oryzae</em>, findings divulged the low rates of adult emergence, low rates of grain consumption and high rates of mortality in the grain of rice varieties, HUR-3022 and Nagina-22. Conversely, <em>S. oryzae</em> adult emergence and grain consumption were found to be high in the grain of Swarna, Sathi, and Pusa basmati-1. Interestingly, the morphological and biochemical composition of the grain was the most important factor in determining resistant or preferred rice varieties to <em>S. oryzae</em>. Among the various physical characteristics investigated, grain hardness was found to have a significant and negative correlation with alive insect, grain weight loss, and preference index. On the other hand, protein and other biochemical grain characteristics were found to have a positive correlation with insect emergence, weight loss, and preference index. In particular, amylose (a key grain component) was found to have a negative correlation with weight loss and insect emergence. The findings of this study suggest that screening of different rice genotypes, for determining grain preference, may be a more viable option to minimize the grain losses during storage caused by <em>S. oryzae.</em> Moreover, the findings of present study could also help to formulate the breeding programs aimed at developing cultivars resistant to this notorious pest.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141481546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency evaluation of ordinary and nanoscale extracts of Agaricus bisporus. on inhibition mycelial growth and aflatoxin production of Aspergillus flavus in maize grain","authors":"Falah Abdul Hassan Hussein,&nbsp;Osama Abdul-Rahman Owied","doi":"10.1016/j.jspr.2024.102374","DOIUrl":"https://doi.org/10.1016/j.jspr.2024.102374","url":null,"abstract":"<div><p>This study aimed to protect maize crops from infection and production of <em>Aspergillus flavus</em> (<em>A. flavus</em>) aflatoxin B1(AFB1), using the ordinary and nanoscale extracts of <em>Agaricus</em> spp. HPLC technology showed that the isolate taken from Musayyib district which coded with (AFA5) was a highest toxin producer. AFB1 at a concentration of 77.02 ppb^. PCR product showed that the amplification of the ITS4- region was successful ITS1 according to the primers used, where a single bundle with a size of 744 bp (base pair) appeared. The isolated and diagnostic results of maize samples collected from separate sites showed the presence of <em>A. flavus</em> and different genera of fungi, namely <em>Aspergillus</em> spp.<em>, Fusarium</em> spp.<em>, Penicillium</em> spp.<em>, and Rhizopus</em> spp.<em>,</em> issued by the genus <em>Aspergillus</em> spp. with frequency rates of 18.33, 38.87, 20.19, 15.92, and 6.69 % respectively. On Potato Dextrose Agar (PDA), inhibitory effect results of <em>Agaricus bisporus</em> (<em>A. bisporus)</em> extract in inhibiting <em>A. flavus</em> growth, with inhibition percentages for the ordinary extract of 43.95, 34.59, and 10.90%, respectively, and for the nano extract 18.74, 43.71, and 76.56 % at 250,500 and 100 ppm respectively. AFB1 production was reduced by adding <em>A. bisporus</em> extracts (ordinary and nano) to Yeast Extract Sucrose Broth (YES) medium by 66% and 72.14%, respectively. Overall, our study presented a new insight for future scientific researches possibility of biocompands production using nanotechnology from natural resources that have the ability to safely environmentally control on mycotoxins production that present in agricultural production especially maize grains and encourage to widespread commercially use.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141481547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality assessment of soybean seeds submitted to industrial seed treatment and stored in a natural and controlled environment","authors":"Josiel Ricardo Toni ,&nbsp;Lauri Lourenço Radünz ,&nbsp;Leandro Galon ,&nbsp;Rafael Gomes Dionello ,&nbsp;Maurício Albertoni Scariot","doi":"10.1016/j.jspr.2024.102372","DOIUrl":"https://doi.org/10.1016/j.jspr.2024.102372","url":null,"abstract":"<div><p>Seed quality is a limiting factor in the search for high soybean yields. However, fungi and insect pests can negatively interfere with their performance, and industrial seed treatment (IST) is a tool used to minimize the attack of pathogens and pests. The treated seeds are often stored until sowing, which can lead to a reduction in their quality. This work aimed to evaluate the physical and physiological quality of soybean seeds submitted to IST, with subsequent storage in a natural and controlled environment. The experiment was conducted in a split-split-plot design in a completely randomized scheme (time x storage systems x IST) with four replications. Soybean cultivar 55I57RSF IPRO was subjected to IST with fungicide (metalaxyl-M, thiabendazole, and fludioxonil) and insecticide (thiamethoxam and cyantraniliprole), combined or not with polymer and dry powder, with subsequent storage in a controlled and natural environment. The moisture content, thousand-seed weights, germination, first germination count, germination speed index, accelerated aging, seedling length, dry matter transfer, and emergence sand test were evaluated. IST did not affect the moisture content and weight of a thousand seeds. However, storage in a controlled environment provided a greater reduction in moisture content and a smaller reduction in the weight of a thousand seeds. IST negatively affected germination, first germination count, germination speed index, and accelerated aging test. IST negatively affected plant length, except in the treatment where only insecticide was applied. The dry mass transfer was negatively affected but with less intensity in seeds subjected only to the application of insecticide or fungicide and the combination of insecticide, fungicide, and polymer. Seed emergence in the sand was negatively affected by all treatments. Regardless of the treatments, all responses evaluated showed a reduction throughout storage, with greater intensity in seeds stored in the natural environment.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141444482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and expression analysis of six small heat shock protein genes in Trogoderma granarium during cold and starvation-induced larval diapause","authors":"Asli Dageri","doi":"10.1016/j.jspr.2024.102368","DOIUrl":"https://doi.org/10.1016/j.jspr.2024.102368","url":null,"abstract":"<div><p>The khapra beetle, <em>Trogoderma granarium</em> Everts (Coleoptera: Dermestidae), is a highly invasive pest infesting stored grains, cereal products, and is recognised as a quarantine pest in many countries. Various stressors can instigate prolonged diapause among insect larvae, increasing the challenge of managing infestation. When the environmental conditions become conducive to development, diapause ceases, and the larva progresses on its developmental course. Members of the Heat Shock Protein (HSP) family play a significant role in protecting insects from the impacts of environmental stressors. In this study, six small heat shock proteins (TsHSP19.8, TsHSP20.5, TsHSP20.6, TsHSP20.8, TsHSP21.2, and TsHSP21.3) were identified and characterized in <em>T. granarium</em>. Transcript abundances of <em>TsHSP</em>s were analysed across distinct phases of larval diapause induced by both cold exposure and starvation stress treatments, alongside various developmental stages. The gene expression patterns of <em>TsHSP20.5</em> and <em>TsHSP21.2</em> exhibited peak mRNA levels during pre-diapause following the cold treatment, while the greatest abundances of <em>TsHSP19.8</em>, <em>TsHSP20.6</em>, <em>TsHSP20.8</em>, and <em>TsHSP21.3</em> were detected in the diapause phases. The highest mRNA abundances of <em>TsHSP20.5</em>, <em>TsHSP20.8</em>, <em>TsHSP21.2</em>, and <em>TsHSP21.3</em> were observed during the diapause phase following the starvation treatment, while it was noted on the first day of the post-diapause phase for <em>TsHSP19.8</em> and <em>TsHSP20.6</em>. The remarkable levels of <em>TsHSP</em>s during diapause might imply their putative role in cold and starvation survival. Significant expression of <em>TsHSP19.8</em>, <em>TsHSP20.6</em>, <em>TsHSP20.8</em>, and <em>TsHSP21.3</em> transcripts was observed in adults, suggesting their potential involvement in developmental processes. Understanding the genetic mechanisms involved in the diapause physiology of <em>T. granarium</em> could offer additional insights for the integrated management of this pest.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141424070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}