{"title":"Isolation and partial characterization of an α-amylase inhibitor present in Phaseolus lunatus seeds and its relationship with the induction of α-amylases in larval Zabrotes subfasciatus","authors":"","doi":"10.1016/j.jspr.2024.102385","DOIUrl":"10.1016/j.jspr.2024.102385","url":null,"abstract":"<div><p>The Mexican bean weevil, <em>Zabrotes subfasciatus</em>, causes significant economic losses by infesting the seeds of three major legume species cultivated in Brazil, namely <em>Phaseolus lunatus</em>, <em>Phaseolus vulgaris</em>, and <em>Vigna unguiculata</em>. α-Amylase inhibitors found in <em>P. vulgaris</em> seeds, known as α-AI-1, are efficient against Old World bruchids but are ineffective against <em>Z. subfasciatus</em>. Exposure to α-AI-1 induces the expression and secretion of two α-amylase isoforms, both insensitive to this inhibitor when compared to the constitutive isoform. Interestingly, this induction was also observed when these insects were cultivated on <em>P. lunatus</em> seeds. In this study, we demonstrated the induction of digestive amylases in <em>Z. subfasciatus</em> on <em>P. lunatus</em> seeds through feeding trials. Separation of <em>P. lunatus</em> seed extracts via ion-exchange chromatography led to the identification of fractions containing inhibitory activity against porcine pancreatic amylase. Enzymatic kinetic parameters revealed the presence of a mixed non-competitive inhibitor in <em>P. lunatus</em> fractions, akin to the pattern observed in <em>P. vulgaris</em>. Furthermore, the protein profiles of both types of seeds were similar, with the enrichment of a 30–32 kD protein, possibly representing a precursor form of the α-amylase inhibitor. Thus, our findings show the presence of an α-AI-1-like inhibitor in <em>P. lunatus</em> seeds, which may play a pivotal role in modulating digestive amylases in <em>Z. subfasciatus</em> larvae.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141949884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pressures measurements of maize grain in cylindrical steel silos and comparison with different international standards","authors":"","doi":"10.1016/j.jspr.2024.102391","DOIUrl":"10.1016/j.jspr.2024.102391","url":null,"abstract":"<div><p>Flat bottom cylindrical silos are employed worldwide to store granular products of high commercial value. However, many gaps still remain in relation to pressure estimation and structural failures still occur. Moreover, there are few full-scale experimental stations in the world to facilitate advances in this line of research. Therefore, in this work, pressures were evaluated in a metallic cylindrical pilot silo that allows geometry adaptations. The pressures were analyzed for two slenderness ratios (2 and 3, respectively) with a flat bottom, using maize, a free-flowing product, as the stored product. The pressures were analyzed during loading and discharge conditions. The experimental results were compared with those recommended by means of the current standards EN 1991–4 and ANSI/ASAE S433.1 and the theory of Jenike et al. (1973). The results obtained show that the discharge pressures for the H/D ratio equal to 2 may exceed those obtained by the calculation method proposed by ANSI/ASAE S433.1, showing that the coefficients used in the standard are not sufficient to promote safety in projects of this configuration. Among the analyzed standards, EN 1991–4:2006 best fitted the experimental discharge pressures for the H/D ratio equal to 3. However, the theory of Jenike et al. (1973) presented theoretical values closer to the experimental ones in all analyzed depths. It is clear that further experimental studies are still needed to understand the underlying physical phenomena and the relationships between the pressures, silo geometry and flow pattern of the stored material.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141959579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of Cinnamomum verum and Syzygium aromaticum extract-loaded liposomes as natural antifungal preservatives in packed fresh pasta","authors":"","doi":"10.1016/j.jspr.2024.102389","DOIUrl":"10.1016/j.jspr.2024.102389","url":null,"abstract":"<div><p>The aim of this research was to the effect of <em>Cinnamomum verum</em> and <em>Syzygium aromaticum</em> extract-loaded liposomes (from 0.1% to 0.5%) on the shelf-life of fresh pasta during 28 days of storage in the refrigerator (4 ± 1 °C). The liposomeadded fresh pasta samples were examined concerning some physical, chemical, sensory, and microbiological properties. According to the results, the addition of extract-loaded liposomes reduced weight increase, volume increase, and also increased cooking loss. The incorporation of <em>Syzygium aromaticum</em>-loaded liposomes resulted in a significantly higher increase in total phenolic content and antioxidant activity of fresh pasta. In which the increase of the total phenolic content and antioxidant activity at the end of storage (28 days) were from 259.20 to 288.13 mg GAE/kg and from 48.65% to 56.79%, respectively. However, moisture content and water uptake demonstrated a reduction following a 28-day storage period. The results of yeast and mold analyses revealed that after 28 days of storage, <em>Syzygium aromaticum</em> liposome-loaded fresh pasta samples had a lower yeast and mold count than other samples. In terms of sensory evaluation, the samples were generally found to be of an acceptable standard, with ratings above a certain average.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141959578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of varietal diets on the effectiveness of modified atmospheric conditions against the life stages of bean beetle, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae)","authors":"","doi":"10.1016/j.jspr.2024.102377","DOIUrl":"10.1016/j.jspr.2024.102377","url":null,"abstract":"<div><p>The bean beetle <em>Acanthoscelides obtectus</em> (Say) (Coleoptera: Chrysomelidae) is one of the most important pests of legumes in the field and storage, it causes losses in kidney bean and cowpea seeds exceeding 16% in one generation. The physical control method using modified atmospheres is an alternative method to toxic gases in storage. This study aimed to determine the sensitivity of <em>A. obtectus</em> stages that fed on two types of hosts (kidney bean seeds and cowpea seeds) to the modified atmosphere using three concentrations of carbon dioxide (20, 40, and 60%) and different exposure periods at 30 °C and 65% R.H. In general, the results showed that <em>A. obtectus</em> stages reared on cowpeas were more sensitive to CO<sub>2</sub> than those reared on kidney beans. Also, the stages are arranged according to their degree of sensitivity to carbon dioxide as follows: adults > eggs > larvae > pupae. According to the LT values, the sensitivity of the external stages of the seeds to carbon dioxide was slightly affected by the type of food, while the internal stages were clearly affected. Complete mortality was achieved after 72 h for adults in all treatments as well as after 7 and 6 days for eggs at 20 and 40% CO<sub>2</sub> in both hosts. The exposure periods required to achieve complete mortality were 12, 11, and 10 days for larvae and 12, 12, and 10 days for pupae produced from cowpea seeds, which increased to 14, 12 and 10 days for larvae and 18, 13, and 12 days for pupae produced from kidney bean seeds when treated with 20, 40, and 60% carbon dioxide, respectively. In conclusion, we recommend using the highest concentration of CO<sub>2</sub> to control all stages of <em>A. obtectus</em> for 10 days in the case of cowpea seeds and 12 days in the case of kidney bean seeds.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141637329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Encountering a dead or live conspecific affects the behavior and longevity of Callosobruchus","authors":"","doi":"10.1016/j.jspr.2024.102387","DOIUrl":"10.1016/j.jspr.2024.102387","url":null,"abstract":"<div><p>Invertebrates including insects have been shown to exhibit a variety of cognitive behaviors and physiological events that indicate interior states similar to human emotion. Therefore, our main question was whether insects have such capacity or not. To shed light on such questions we used the seed beetle <em>Callosobruchus maculatus</em> to investigate the behavior of the insects that were raised in complete isolation and had never seen any other conspecific and to see how they might respond when they encountered their live or dead conspecific for a short time (10 min) and then biological parameters and copulation behaviors were compared with the controls. Results showed that having a brief encounter with a dead conspecific caused different sex-specific biological and behavioral changes. Males who saw another live or dead conspecific and then did not mate during their lifetime had a shorter lifespan than males who mated once and those who had never seen another conspecific at all. A deprivation that insects didn't know about was less damaging. In males, sexual deprivation had a more substantial impact than perceived social deprivation. In contrast, encountering a dead conspecific increased female longevity. Males who observed a dead female body an hour before mating paid less attention to females when a female was put into their niches and male mating latency increased. Males also showed necrophiliac behavior. These results lead us to conclude that this insect experiences some type of cognition although it is too early to conclude in definite and it needs more investigation.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141638136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Monitoring and predicting the quality of soybeans for different drying and storage technologies on a real scale using sensors and Machine Learning models","authors":"","doi":"10.1016/j.jspr.2024.102386","DOIUrl":"10.1016/j.jspr.2024.102386","url":null,"abstract":"<div><p>The application of monitoring techniques associated with artificial intelligence in grain drying and storage operations can assist in decision-making processes, preventing deterioration. Therefore, the objective of this study was to evaluate the effects of different drying technologies (continuous drying and dryer-silos) and storage methods (vertical and horizontal silos) on the quality of soybeans associated with Machine Learning algorithms to predict changes in grain quality. The environmental and intergranular variables monitored during the processes were correlated with physical and chemical quality parameters of the grains for prediction through Machine Learning models. The equilibrium moisture content above 12% increased the respiration of the stored grain mass to CO<sub>2</sub> concentration levels above 600 ppm, causing changes in the soybean quality from 4 to 14% dry matter loss, and reducing the apparent mass specific at 650 kg m<sup>−3</sup>, germination at 60%, oil yield reduced at 18%, and crude protein at 34%, as well as increased the electrical conductivity at 500 mS cm<sup>−1</sup> g<sup>−1</sup>, the acidity oil increased at 7 mg KOH g<sup>−1</sup>. It was observed that grain subjected to drying and storage conditions in dryer-silos maintained the highest grain quality at the end of the process. Although there were differences related on the applied drying and storage technology regarding changes in grain quality, it was noticed that the Artificial Neural Networks model demonstrated superior performance in predicting grain quality. Thus, it is recommended to conduct post-harvest drying of soybeans and subsequent grain storage in dryer-silos, while monitoring environmental and intergranular variables. This approach is advised to be coupled with the utilization of Artificial Neural Networks models to anticipate losses and enhance grain conversation with greater efficiency.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141638139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation of preservation paper containing essential oil microemulsion and its application in maintain the quality of post-harvest peaches","authors":"","doi":"10.1016/j.jspr.2024.102388","DOIUrl":"10.1016/j.jspr.2024.102388","url":null,"abstract":"<div><p>Post-harvest peaches are highly susceptible to fungal infections leading to spoilage. This study investigates the utilization of three edible plant essential oils (EOs) with antifungal properties for the preservation of peaches, along with an evaluation of their preservative efficacy. Initially, GC-MS was employed to identify the primary constituents of the EOs, revealing a predominance of phenols, alcohols, hydrocarbons and aldehydes. Principal component analysis was conducted to compare the chemical compositions of the EOs from the plants used in this study with those obtained from other regions. This analysis facilitates the quality assessment of the plant materials in the later stages of the research. <em>In vitro</em> antimicrobial assays indicated that the three EOs independently exhibited their respective antifungal activities against 7 fungi responsible for peach spoilage. The Response Surface Methodology was then applied to optimize the blend ratio of the composite EOs (CEOs) for maximum antifungal efficacy. Concurrently, this study validated the rationality of a second-order polynomial model that correlates the peach decay index with the concentration of the CEOs. To enhance the bioavailability of the CEOs, a pseudo-ternary phase diagram was employed to formulate the CEOs into a microemulsion. Based on the RSM optimization results for the CEOs, the optimal mass ratio of the components within the microemulsion was determined. Using this microemulsion as a base, preservative films were developed that demonstrated a significant preservation effect on peaches, highlighting the immense potential of this research in the food industry.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141637330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Transgenerational effects of sublethal deltamethrin exposure on development and repellency behaviour in Callosobruchus chinensis","authors":"","doi":"10.1016/j.jspr.2024.102379","DOIUrl":"10.1016/j.jspr.2024.102379","url":null,"abstract":"<div><p><em>Callosobruchus chinensis</em>, belongs to the Chrysomelidae family, is a major pest of pulse grains. It causes 60% weight loss of the pulses, making them inappropriate for planting or human consumption. <em>C. chinensis</em> is commonly controlled with insecticides; however, these insecticides have negative effects on the environment and non-target organisms. Moreover, repeated exposure to the insecticide causes the resistance in the <em>C. chinensis</em>. Initially the lethal concentration of deltamethrin (LC<sub>50</sub>) was obtained by the probit analysis. Later the study aimed to examine the impact of a sublethal concentration of deltamethrin on various developmental characteristics and repellency behaviour throughout six successive generations of the <em>C. chinensis</em>. For that, two concentration were selected <em>viz</em> sublethal low concentration (1/20<sup>th</sup>) and sublethal high concentration (1/5<sup>th</sup>) of the LC<sub>50</sub> compared to control. Both concentrations significantly reduce the life table parameters like egg count, total hatching, total development duration, total emergence and adult longevity in the first generation but an increasing trend in all the parameters was observed in subsequent generations (progeny). Additionally, the repellency was maximum in 1<sup>st</sup> generation but it decreases with the exposure time and successive generations which shows that it was time and generation dependent. The findings indicate that although there are predominantly adverse effects within the generation, exposure to deltamethrin at sublethal levels over the generations can lead to beneficial effects in the offspring, enhancing their ability to withstand insecticides.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141623009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mycotoxigenic fungal growth within stored maize (Zea mays L.) at different storage altitudes and durations","authors":"","doi":"10.1016/j.jspr.2024.102383","DOIUrl":"10.1016/j.jspr.2024.102383","url":null,"abstract":"<div><p>In this study, the interactive effect of maize (<em>Zea mays</em> L.) storage altitude, storage duration, and initial moisture content on the invasion of mycotoxigenic fungi on maize stored within woven polypropylene bags was evaluated. The direct plating method on potato dextrose agar media was used for the isolation of maize kernel fungi, and the identification was done morphologically. The study result revealed that <em>Aspergillus</em> spp., <em>Fusarium</em> spp., and <em>Aspergillus flavus</em> were identified at all storage altitudes (1500, 1800, and 2200 m above sea level (m.a.s.l.)), and the level of fungal growth was affected by storage duration and initial moisture content. There was a relatively higher density of <em>Aspergillus flavus</em> at lower altitudes. <em>Aspergillus flavus</em> growth was significantly increased by prolonging maize storage duration at 1500 m.a.s.l. altitude; kernel infection increased from 33% to 47% between the 4th and 8th months of storage. Similarly, there was a higher incidence of <em>Aspergillus</em> spp. at the lower altitude; after 8 months of storage, the relative density of <em>Aspergillus</em> spp. was 69% at 1500 m.a.s.l. altitude. After 8 months of storage, the highest kernel infection with <em>Fusarium</em> spp. (53%) was observed at 2200 m.a.s.l. (the highest altitude). There was a decrease in <em>Fusarium</em> spp. kernel infection from the 4th month (27%) to the 8th month (7%) at 1500 m.a.s.l. altitude. The relative density of the fungi increased with an increment in the initial storage moisture content of maize. Hence, the existence of <em>Aspergillus</em> spp., <em>Aspergillus flavus</em>, and <em>Fusarium</em> spp. in maize stored with different initial moisture contents (12%–15%), various sites of an agro-ecological zone, and storage durations emphasizes the need to utilize climate-smart post-harvest technologies for storing maize for more than 4 months in woven polypropylene bags, particularly at lower altitudes (1500 m.a.s.l.) to minimize aflatoxin contamination.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141623007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quality of honey from Tubuna bees (Scaptotrigona bipunctata) fresh, thermally treated and during storage","authors":"","doi":"10.1016/j.jspr.2024.102376","DOIUrl":"10.1016/j.jspr.2024.102376","url":null,"abstract":"<div><p>Intrinsic characteristics, such as the presence of sugars and high levels of moisture and water activity, give stingless bee honey a greater tendency to fermentation than <em>Apis mellifera</em> honey. Thus, this study aimed to evaluate the physicochemical, microbiological, and antioxidant parameters of honey sourced from Tubuna bees (<em>Scaptotrigona bipunctata</em>) in its fresh state, following thermal treatment, and during storage. Samples were collected from three meliponaries in the South of Brazil. Thermal treatment involved a 3 min immersion at 65 °C, and storage occurred at ambient temperature. Parameters assessed included moisture, free acidity, pH, water activity, soluble solids, reducing sugars, apparent sucrose, hydroxymethylfurfural, color, ash, mesophilic aerobic bacteria, molds, yeasts, <em>Escherichia coli</em>, and antioxidant activity (DPPH and ABTS). Thermally treated honey demonstrated improved moisture stability, acidity, water activity, and increased color during storage. There were no significant differences in pH, soluble solids, hydroxymethylfurfural, bacteria, molds, yeasts, and antioxidant activity between thermally treated and fresh honey, and during storage. Both fresh and thermally treated <em>S. bipunctata</em> honey samples were free from <em>E. coli</em> and maintained values within state legislation limits for moisture, sugars, hydroxymethylfurfural, ash, molds, and yeasts during storage. However, the microorganism count was reduced, and an increase in antioxidant activity after 360 days was observed. The treatment in a water bath at 65 °C for 3 min preserved the physicochemical, microbiological, and antioxidant quality of <em>Scaptotrigona bipunctata</em> honey during storage. The results hold promise for supporting “<em>meliponicultores”</em>, offering market opportunities, ensuring product quality, and fostering sustainability while preserving local biodiversity and culture.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141623008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}