Journal of Pharmacy and Pharmacology最新文献

{"title":"Development of antibacterial coatings for endotracheal tubes with enhanced antibacterial release properties through combined antibiotic loading.","authors":"Matthew P Wylie, Jia Li, Grainne Murphy, Jasmine Ross, Jane Burns, David S Jones, Colin P McCoy","doi":"10.1093/jpp/rgaf078","DOIUrl":"https://doi.org/10.1093/jpp/rgaf078","url":null,"abstract":"<p><strong>Background: </strong>Ventilator-associated pneumonia (VAP) is a significant cause of patient morbidity and mortality worldwide. This study describes a strategy to develop antibacterial hydrogel-based coating for endotracheal tubes, composed of 2-hydroxyethyl methacrylate and methacrylic acid, capable of simultaneously delivering a combination of antibacterial agents to mitigate VAP.</p><p><strong>Methods: </strong>Copolymer hydrogels were loaded with gentamicin, levofloxacin, and benzalkonium chloride (BAK), and their drug release profiles, antibacterial activity, and duration were determined.</p><p><strong>Key findings: </strong>More than 95% and 99.99% reduction of bacterial adherence was observed for all the antibiotic-containing hydrogels after 4 and 24 h, respectively. Hydrogels loaded with BAK provided protection against Staphylococcus aureus for more than 30 days and hydrogels loaded with levofloxacin exhibited more than 10 days of persistence against Pseudomonas aeruginosa. Dual loading of levofloxacin and BAK showed additive effects against bacteria and provided delayed release of both agents.</p><p><strong>Conclusions: </strong>The use of combined antibiotic-loaded hydrogel coatings provides a promising approach to combating the development of VAP.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical efficacy of topical vancomycin powder in the prevention of surgical site infection in total joint arthroplasty: a systematic review and meta-analysis.","authors":"Jingya Liu, Na Cao, Kuimeng Li, Yan Zhang","doi":"10.1093/jpp/rgaf077","DOIUrl":"https://doi.org/10.1093/jpp/rgaf077","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the clinical efficacy of the topical application of vancomycin powder in the prevention of surgical site infection after total joint arthroplasty (TJA).</p><p><strong>Method: </strong>Five English-language databases and four Chinese-language databases were systematically searched from the establishment of the database to 31 January 2024, and a meta-analysis was performed using Review Manager 5.3 software.</p><p><strong>Results: </strong>A total of 34 314 patients from 17 studies were included in this meta-analysis. The results showed that, compared with the control group, the topical application of vancomycin powder in the experimental group reduced the overall incidence of prosthetic joint infection (PJI) after TJA surgery (P < .001). Within the subdivisions of the surgical field, in total knee arthroplasty, the incidence of PJI was significantly lower in the experimental group using vancomycin powder than in the control group not using this powder (P < .001). In total hip arthroplasty, the incidence of PJI was also significantly lower in the experimental group than in the control group (P = .004). However, 11 studies mentioned adverse reactions, and the results showed that the incidence of adverse reactions in the experimental group increased compared with the control group (P = .008).</p><p><strong>Conclusion: </strong>The application of vancomycin powder appears to significantly reduce PJI risk in TJA patients; however, an increased incidence of postoperative wound complications warrants cautious clinical consideration.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aucubin inhibits the activity of lung cancer stem-like cells by targeting degradation of β-catenin.","authors":"Qian Li, Hao Xie, Linli Li, Wenying Yan, Guoqiang Liu","doi":"10.1093/jpp/rgaf081","DOIUrl":"https://doi.org/10.1093/jpp/rgaf081","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the antitumor effects of aucubin (AC) in non-small cell lung cancer (NSCLC) and uncover its plausible mechanism against lung cancer stem-like cells (LCSCs).</p><p><strong>Methods: </strong>In vitro experiments included MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a reagent commonly used for cell viability assay) and colony formation assays to assess anti-proliferative effects on A549 and NCI-H1975 lung cancer cell lines, wound healing and Transwell invasion assays to evaluate inhibition of cell migration and invasion, tumorsphere-formation experiments to detect changes in NSCLC cell stemness, as well as Western blot and quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses to measure the expression of LCSC markers (CD44, CD133, Oct4, and Nanog). In vivo experiments were conducted to observe the impact of AC on NSCLC metastasis and mouse survival rates. Further mechanistic studies involved transcriptomic gene set enrichment analysis, Western blot, qRT-PCR, molecular docking, and Surface Plasmon Resonance (SPR) methods to investigate how AC directly targets β-catenin and promotes its ubiquitin-mediated degradation.</p><p><strong>Key findings: </strong>AC exerted significant anti-proliferative effects on A549 and NCI-H1975 cells, inhibited cancer cell migration and invasion, reduced the stemness of NSCLC cells, and markedly downregulated the expression of LCSC markers in vitro. In vivo, AC treatment significantly reduced NSCLC metastasis and improved mouse survival rates.Mechanistically, AC blocked the WNT (Wingless-related integration site, a family of secreted lipid-modified signaling glycoproteins that play crucial roles in embryonic development, tissue homeostasis, and regeneration) signaling pathway by downregulating β-catenin and c-Myc expression. It directly targeted β-catenin, promoting its degradation via the ubiquitin-proteasome pathway.</p><p><strong>Conclusions: </strong>This study uncovers a novel anti-LCSC mechanism of AC, offers alternative strategies for NSCLC treatment, and provides innovative lead compounds for the development of drugs targeting lung cancer stem cells.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of benznidazole orally disintegrating tablets for paediatric patients.","authors":"Fermín Cañete Alberdi, María F Filia, Laura D Simionato, Carlos A Sandrone, Daniel R Vega, Javier Oppezzo, Christian Höcht, Diego A Chiappetta, Viviana S L Mouriño, Héctor J Prado","doi":"10.1093/jpp/rgaf076","DOIUrl":"https://doi.org/10.1093/jpp/rgaf076","url":null,"abstract":"<p><strong>Objectives: </strong>To develop the orphan drug benznidazole (BNZ) in orally disintegrating tablets, for the neglected disease American Trypanosomiasis (Chagas disease) therapy. Although children are highly affected by this disease, there are no specific commercial pharmaceutical preparations for this age group in Argentina and in many other countries.</p><p><strong>Methods: </strong>In the production process, co-milling in a ball mill was applied to enhance dissolution rates, followed by direct compression. Taste preference and taste masking experiments were conducted in a test panel of adult volunteers. The tablets were fully characterized and their stability and bioavailability determined.</p><p><strong>Key findings: </strong>The tablets complied with all the quality control prerequisites, their disintegration time was 30 s, and as a consequence of the intimate mixture with hydrophilic excipients and particle size reduction, BNZ dissolution was improved, reaching 75% in 0.1 N hydrochloric acid and 62% in simulated salivary fluid, after 5 min. X-ray diffraction studies showed that BNZ maintained its original crystalline state (form I) in the tablets. The ODTs remained stable for at least 1 year. Oral bioavailability of BNZ of suspensions obtained from the prepared ODTs was comparable with that of pulverized commercial tablets.</p><p><strong>Conclusions: </strong>The developed tablets may improve paediatric Chagas disease therapeutics.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of polyphenolic-enriched Pleurospermum candollei extract against doxorubicin-induced cardiovascular disease in rats: modulation of oxidative stress, inflammation, and apoptosis.","authors":"Mehreen Fatima, Muhammad Rashid Khan, Lamya Ahmed Al-Keridis, Nawaf Alshammari, Mitesh Patel, Mohd Adnan","doi":"10.1093/jpp/rgaf042","DOIUrl":"10.1093/jpp/rgaf042","url":null,"abstract":"<p><strong>Objectives: </strong>Pleurospermum candollei is used to treat abdominal problems, heart diseases, gastric issues, and cerebral disorders. This study aimed to evaluate the therapeutic potential of P. candollei aqueous extract (PCA) against Doxorubicin (DOX)-induced cardiac failure in rats.</p><p><strong>Methods: </strong>The composition of the extract was determined by quantifying polyphenols using HPLC-DAD, and antioxidant capacity was evaluated through the FRAP assay. Fifty-four rats were divided into nine groups, and cardiac injury was induced by DOX (10 mg/kg body weight). PCA extract (200, 400, and 600 mg/kg body weight) was administered orally to treat cardiotoxicity. Serum markers, antioxidants, inflammatory, fibrotic, and apoptotic genes, and histological alterations were assessed.</p><p><strong>Key findings: </strong>Examination confirmed the presence of various polyphenols in the plant extract. PCA extract administration to rats reduced the DOX-instigated elevation in CK, LDH, and triglycerides concentrations in serum and restored the histopathological changes. Similarly, PCA extract administration normalized the levels of antioxidants and pro-inflammatory markers and reduced the expression of apoptotic and fibrosis markers.</p><p><strong>Conclusion: </strong>PCA extract exhibited significant antioxidants and cardioprotective activities in rats. The cardioprotective and anti-inflammatory effects of PCA may be attributed to its high content of polyphenolics, and it acts as a promising therapeutic source against heart failure.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1277-1290"},"PeriodicalIF":3.2,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction of: The antiproliferative and apoptotic effects of apigenin on glioblastoma cells.","authors":"","doi":"10.1093/jpp/rgaf049","DOIUrl":"10.1093/jpp/rgaf049","url":null,"abstract":"","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1292"},"PeriodicalIF":3.2,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compare Phellodendri Chinensis Cortex before and after salt-water processing to ameliorate diabetic nephropathy via metabolomics and microbiome analysis.","authors":"Huangyao Zhu, Shufan Jin, Siqi Fan, Wanqiu Liu, Yizhu Wang, Jingwen Ha, Yuxuan Lu, Zheling Li, Ma Mi, Jie Zhang, Wenyuan Liu, Lamu Yixi, Feng Feng, Jian Xu","doi":"10.1093/jpp/rgaf033","DOIUrl":"10.1093/jpp/rgaf033","url":null,"abstract":"<p><strong>Objectives: </strong>The study aims to investigate the therapeutic effects and the underlying mechanisms of Phellodendri Chinensis Cortex (PC) and its salt-water processed form (SPC) on diabetic nephropathy (DN).</p><p><strong>Methods: </strong>Histopathological examination, biochemical evaluation immunohistochemistry/immunofluorescence assay were used to compare the effects of PC and SPC on DN. Intestinal microbiota was sequenced by 16S rDNA, serum differential metabolites were identified by UPLC-Q/TOF-MS to elucidate the mechanism.</p><p><strong>Results: </strong>PC and SPC could improve renal function, reduce blood glucose, proteinuria, inflammation, and oxidative stress, and restoring gut microbiota homeostasis in DN rats, with SPC showing superior efficacy. PC influenced 8 metabolites, primarily in glycerolipid metabolism and pentose and glucuronate interconversions, whereas SPC affected 30 metabolites, predominantly in pathways closely associated with glucose and lipid metabolism, including pentose and glucuronate interconversions, ether lipid metabolism and glycerophospholipid metabolism. Correlation analysis identified specific gut microbiota, such as Enterobacteriaceae, Muribaculaceae, and Lachnospiraceae, as highly correlated with the metabolic effects induced by PC and SPC.</p><p><strong>Conclusion: </strong>The study provides evidence that PC and SPC have a beneficial effect on DN, with SPC exhibiting enhanced therapeutic potential. Furthermore, SPC could better restore gut microbiota diversity and structure, and improved glucose and lipid metabolism.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1175-1191"},"PeriodicalIF":3.2,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Novel oral fast-disintegrating drug delivery devices with predefined inner structure fabricated by Three-Dimensional Printing.","authors":"","doi":"10.1093/jpp/rgaf047","DOIUrl":"10.1093/jpp/rgaf047","url":null,"abstract":"","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1291"},"PeriodicalIF":3.2,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk stratification of AUC upward deviation in patients with low serum creatinine levels treated with vancomycin: a multicenter retrospective study.","authors":"Tomoyuki Ishigo, Ayako Suzuki, Yuta Ibe, Satoshi Fujii, Masahide Fukudo, Hiroaki Yoshida, Hiroaki Tanaka, Hisato Fujihara, Fumihiro Yamaguchi, Fumiya Ebihara, Takumi Maruyama, Yukihiro Hamada, Masaru Samura, Fumio Nagumo, Toshiaki Komatsu, Atsushi Tomizawa, Akitoshi Takuma, Hiroaki Chiba, Yoshifumi Nishi, Yuki Enoki, Kazuaki Taguchi, Kazuaki Matsumoto","doi":"10.1093/jpp/rgaf038","DOIUrl":"10.1093/jpp/rgaf038","url":null,"abstract":"<p><strong>Introduction: </strong>In patients with diminished muscle mass, serum creatinine (SCr) levels may be misleadingly low, potentially leading to overestimations of kidney function and unexpectedly high blood levels of vancomycin. This study aimed to identify factors contributing to this discrepancy and develop a flowchart for stratifying the risk of excessive vancomycin exposure, measured as an upward deviation in the area under the concentration-time curve (AUC) in patients with low SCr levels.</p><p><strong>Methods: </strong>We analyzed data from patients who received vancomycin and had an SCr value <0.6 mg/dL. The discrepancy between the AUC24-48 h initial dosing and AUC24-48 h TDM was calculated; a ratio (AUC24-48 h TDM/AUC24-48 h initial dosing) higher than 1.2 defined an upward deviation in the AUC. A decision tree model was constructed using classification and regression tree algorithms.</p><p><strong>Results: </strong>Among the 95 patients (median age [interquartile range], 69 [58, 80] years; 68% female), the upward AUC deviation was 40% (38/95). Three factors (creatinine clearance: 115 ml/min, age: 69 years, and blood urea nitrogen/SCr: 17) were selected for the decision flowchart and included in four subgroups.</p><p><strong>Conclusion: </strong>We developed a flowchart to identify patients with low SCr levels whose AUC at therapeutic drug monitoring deviated upward by >20% from the predicted AUC.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1255-1263"},"PeriodicalIF":3.2,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naochuxue formula attenuates early brain injury following subarachnoid hemorrhage by inhibiting neuronal apoptosis via network pharmacology and in vivo experiments.","authors":"Huiying Yan, Limei Fang, Chang Qi, Mingquan Li, Qile Song, Haipeng Sun, Bo Liu, Lina Feng","doi":"10.1093/jpp/rgaf035","DOIUrl":"10.1093/jpp/rgaf035","url":null,"abstract":"<p><strong>Objectives: </strong>Investigate Naochuxue formula's mechanism in the treatment of subarachnoid hemorrhage.</p><p><strong>Methods: </strong>Analyzed Naochuxue formula's active components via nontargeted metabolomics, and predicted the core targets using network pharmacology. Sprague‒Dawley rats were randomly divided into six groups: sham, model, Naochuxue formula low, medium and high dose groups, and edaravone. Neurological deficits were assessed using the modified Garcia score and tissue damage was assessed by measuring the brain water content. Blood‒brain barrier permeability was assessed using the Evans blue procedure and pathological changes in the lesion site were observed through HE staining and Nissl staining. TUNEL staining and Caspase-3 immunofluorescence were used to observe the apoptosis of neurons in the hippocampus. The distribution and expression of p-PI3K and p-AKT were determined using immunohistochemistry. The expression of the apoptosis-related genes Caspase-3, Bcl-2, and Bax was determined using RT‒PCR.</p><p><strong>Key findings: </strong>Compared with the model group, rats in the high-dose Naochuxue formula group exhibited significant improvements in neurological defects, brain histopathology, blood‒brain barrier permeability and brain edema on Day 3 posttreatment, downregulated Bax and Caspase-3 expression, and significantly upregulated p-PI3K, p-AKT, and Bcl-2 expression (all P < 0.05).</p><p><strong>Conclusions: </strong>High-dose formula for 3 days activated PI3K/AKT signaling pathway, inhibitd neuronal apoptosis, and exerted neuroprotective effects.</p>","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"1203-1221"},"PeriodicalIF":3.2,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}