Compare Phellodendri Chinensis Cortex before and after salt-water processing to ameliorate diabetic nephropathy via metabolomics and microbiome analysis.

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-04 DOI:10.1093/jpp/rgaf033

Huangyao Zhu, Shufan Jin, Siqi Fan, Wanqiu Liu, Yizhu Wang, Jingwen Ha, Yuxuan Lu, Zheling Li, Ma Mi, Jie Zhang, Wenyuan Liu, Lamu Yixi, Feng Feng, Jian Xu

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引用次数: 0

Abstract

Objectives: The study aims to investigate the therapeutic effects and the underlying mechanisms of Phellodendri Chinensis Cortex (PC) and its salt-water processed form (SPC) on diabetic nephropathy (DN).

Methods: Histopathological examination, biochemical evaluation immunohistochemistry/immunofluorescence assay were used to compare the effects of PC and SPC on DN. Intestinal microbiota was sequenced by 16S rDNA, serum differential metabolites were identified by UPLC-Q/TOF-MS to elucidate the mechanism.

Results: PC and SPC could improve renal function, reduce blood glucose, proteinuria, inflammation, and oxidative stress, and restoring gut microbiota homeostasis in DN rats, with SPC showing superior efficacy. PC influenced 8 metabolites, primarily in glycerolipid metabolism and pentose and glucuronate interconversions, whereas SPC affected 30 metabolites, predominantly in pathways closely associated with glucose and lipid metabolism, including pentose and glucuronate interconversions, ether lipid metabolism and glycerophospholipid metabolism. Correlation analysis identified specific gut microbiota, such as Enterobacteriaceae, Muribaculaceae, and Lachnospiraceae, as highly correlated with the metabolic effects induced by PC and SPC.

Conclusion: The study provides evidence that PC and SPC have a beneficial effect on DN, with SPC exhibiting enhanced therapeutic potential. Furthermore, SPC could better restore gut microbiota diversity and structure, and improved glucose and lipid metabolism.

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通过代谢组学和微生物组学分析比较黄柏皮盐水处理前后对糖尿病肾病的改善作用。

目的：探讨黄柏皮（PC）及其盐水加工物（SPC）对糖尿病肾病（DN）的治疗作用及其机制。方法：采用组织病理学检查、生化评价、免疫组化/免疫荧光法比较PC和SPC对DN的影响。采用16S rDNA对肠道菌群进行测序，UPLC-Q/TOF-MS对血清差异代谢物进行鉴定，以阐明其作用机制。结果：PC和SPC均能改善DN大鼠肾功能，降低血糖、蛋白尿、炎症和氧化应激，恢复肠道菌群稳态，其中SPC效果更佳。PC影响了8种代谢物，主要是甘油脂代谢和戊糖与葡萄糖酸盐的相互转化，而SPC影响了30种代谢物，主要是与葡萄糖和脂质代谢密切相关的途径，包括戊糖和葡萄糖酸盐的相互转化、醚脂质代谢和甘油磷脂代谢。相关分析发现，特定的肠道微生物群，如肠杆菌科、Muribaculaceae和Lachnospiraceae，与PC和SPC诱导的代谢效应高度相关。结论：本研究证明了PC和SPC对DN的有益作用，其中SPC具有增强的治疗潜力。此外，SPC能更好地恢复肠道菌群的多样性和结构，改善糖脂代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pharmacy and Pharmacology 医学-药学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.