Journal of Pharmacy and Pharmacology最新文献

Gold nanoparticle/silk fibroin-based nanofiber enhances skin regeneration. 金纳米粒子/丝素纳米纤维增强皮肤再生。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-16 DOI: 10.1093/jpp/rgaf025

Ozan Ozcan, Elif Tufan, Aleyna Muhan, Esin Ak, Göksel Şener, Tugba Tunali-Akbay

{"title":"Gold nanoparticle/silk fibroin-based nanofiber enhances skin regeneration.","authors":"Ozan Ozcan, Elif Tufan, Aleyna Muhan, Esin Ak, Göksel Şener, Tugba Tunali-Akbay","doi":"10.1093/jpp/rgaf025","DOIUrl":"https://doi.org/10.1093/jpp/rgaf025","url":null,"abstract":"Objectives: The aim of this study was to determine the wound-healing potential of gold nanoparticles and silk fibroin-based nanofiber produced by green chemistry.Methods: The electrospinning method was used to prepare the nanofiber. Twenty rats were exposed to a 7-day treatment period and another 20 rats were exposed to a 21-day treatment period. Rat groups were control, silver, silk fibroin, and silk + gold nanoparticle groups for each period. The effect of the gold nanoparticle/silk fibroin-based nanofiber was examined in skin samples by using biochemical and histological analysis. In biochemical analysis, skin oxidant and antioxidant parameters were determined.Key findings: Parameters indicating skin damage returned to their previous levels 7 and 21 days after the wound formation using gold nanoparticle/silk fibroin-based nanofiber. Gold nanoparticle/silk fibroin-based nanofiber initiated hair follicle formation at the wound site and accelerated the re-epithelialization process.Conclusions: It was found that the nanofiber prepared by adding gold nanoparticles to silk fibroin had better wound-healing properties than silk fibroin nanofibers without gold nanoparticles.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk stratification of AUC upward deviation in patients with low serum creatinine levels treated with vancomycin: a multicenter retrospective study. 万古霉素治疗低血清肌酐水平患者AUC向上偏移的风险分层：一项多中心回顾性研究

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-16 DOI: 10.1093/jpp/rgaf038

Tomoyuki Ishigo, Ayako Suzuki, Yuta Ibe, Satoshi Fujii, Masahide Fukudo, Hiroaki Yoshida, Hiroaki Tanaka, Hisato Fujihara, Fumihiro Yamaguchi, Fumiya Ebihara, Takumi Maruyama, Yukihiro Hamada, Masaru Samura, Fumio Nagumo, Toshiaki Komatsu, Atsushi Tomizawa, Akitoshi Takuma, Hiroaki Chiba, Yoshifumi Nishi, Yuki Enoki, Kazuaki Taguchi, Kazuaki Matsumoto

{"title":"Risk stratification of AUC upward deviation in patients with low serum creatinine levels treated with vancomycin: a multicenter retrospective study.","authors":"Tomoyuki Ishigo, Ayako Suzuki, Yuta Ibe, Satoshi Fujii, Masahide Fukudo, Hiroaki Yoshida, Hiroaki Tanaka, Hisato Fujihara, Fumihiro Yamaguchi, Fumiya Ebihara, Takumi Maruyama, Yukihiro Hamada, Masaru Samura, Fumio Nagumo, Toshiaki Komatsu, Atsushi Tomizawa, Akitoshi Takuma, Hiroaki Chiba, Yoshifumi Nishi, Yuki Enoki, Kazuaki Taguchi, Kazuaki Matsumoto","doi":"10.1093/jpp/rgaf038","DOIUrl":"https://doi.org/10.1093/jpp/rgaf038","url":null,"abstract":"Introduction: In patients with diminished muscle mass, serum creatinine (SCr) levels may be misleadingly low, potentially leading to overestimations of kidney function and unexpectedly high blood levels of vancomycin. This study aimed to identify factors contributing to this discrepancy and develop a flowchart for stratifying the risk of excessive vancomycin exposure, measured as an upward deviation in the area under the concentration-time curve (AUC) in patients with low SCr levels.Methods: We analyzed data from patients who received vancomycin and had an SCr value <0.6 mg/dL. The discrepancy between the AUC24-48 h initial dosing and AUC24-48 h TDM was calculated; a ratio (AUC24-48 h TDM/AUC24-48 h initial dosing) higher than 1.2 defined an upward deviation in the AUC. A decision tree model was constructed using classification and regression tree algorithms.Results: Among the 95 patients (median age [interquartile range], 69 [58, 80] years; 68% female), the upward AUC deviation was 40% (38/95). Three factors (creatinine clearance: 115 ml/min, age: 69 years, and blood urea nitrogen/SCr: 17) were selected for the decision flowchart and included in four subgroups.Conclusion: We developed a flowchart to identify patients with low SCr levels whose AUC at therapeutic drug monitoring deviated upward by >20% from the predicted AUC.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Novel oral fast-disintegrating drug delivery devices with predefined inner structure fabricated by Three-Dimensional Printing. 修正：用三维打印技术制造具有预定内部结构的新型口服快速崩解给药装置。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-13 DOI: 10.1093/jpp/rgaf047

引用次数: 0

Anticancer potential of PEDF peptides. PEDF肽的抗癌潜力。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-13 DOI: 10.1093/jpp/rgaf043

Enas Bani-Ahmad, Joshua Dass, Crispin R Dass

{"title":"Anticancer potential of PEDF peptides.","authors":"Enas Bani-Ahmad, Joshua Dass, Crispin R Dass","doi":"10.1093/jpp/rgaf043","DOIUrl":"https://doi.org/10.1093/jpp/rgaf043","url":null,"abstract":"Objectives: Pigment epithelium-derived factor (PEDF) has demonstrated a wide range of activities, the most notable of which is its role in cancer.Methods: Articles were sourced from Scopus with the following keywords-PEDF, peptide(s), cancer, tumour, and tumour. There was no limit set on date of publication, and the language of publication was set to English.Key findings: Researchers have found two functional epitopes in the PEDF sequence: a 34-mer peptide that mainly inhibits angiogenesis and a 44-mer peptide that mainly promotes differentiation and neurotrophic functions in certain cell lines. Furthermore, studies have demonstrated that shorter peptides in the 34-mer significantly contribute to its angiogenic activity. PEDF peptide functions as an anticancer agent through various mechanisms. The most salient feature is the blockade of angiogenesis by reducing VEGF levels. Angiogenesis is critical in tumour expansion, and it is known as the process whereby new blood vessels are formed from capillaries.Conclusions: Researchers have studied several PEDF peptides in various types of cancer, including ovarian, breast, lung, osteosarcoma, and myeloma. This underscores the potential significance of the various PEDF peptides, given their known ability to influence angiogenesis and other biological processes.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of polyphenolic-enriched Pleurospermum candollei extract against doxorubicin-induced cardiovascular disease in rats: modulation of oxidative stress, inflammation, and apoptosis. 富含多酚的胸香菇提取物对阿霉素诱导的大鼠心血管疾病的保护作用：氧化应激、炎症和细胞凋亡的调节。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-12 DOI: 10.1093/jpp/rgaf042

Mehreen Fatima, Muhammad Rashid Khan, Lamya Ahmed Al-Keridis, Nawaf Alshammari, Mitesh Patel, Mohd Adnan

{"title":"Protective effects of polyphenolic-enriched Pleurospermum candollei extract against doxorubicin-induced cardiovascular disease in rats: modulation of oxidative stress, inflammation, and apoptosis.","authors":"Mehreen Fatima, Muhammad Rashid Khan, Lamya Ahmed Al-Keridis, Nawaf Alshammari, Mitesh Patel, Mohd Adnan","doi":"10.1093/jpp/rgaf042","DOIUrl":"https://doi.org/10.1093/jpp/rgaf042","url":null,"abstract":"Objectives: Pleurospermum candollei is used to treat abdominal problems, heart diseases, gastric issues, and cerebral disorders. This study aimed to evaluate the therapeutic potential of P. candollei aqueous extract (PCA) against Doxorubicin (DOX)-induced cardiac failure in rats.Methods: The composition of the extract was determined by quantifying polyphenols using HPLC-DAD, and antioxidant capacity was evaluated through the FRAP assay. Fifty-four rats were divided into nine groups, and cardiac injury was induced by DOX (10 mg/kg body weight). PCA extract (200, 400, and 600 mg/kg body weight) was administered orally to treat cardiotoxicity. Serum markers, antioxidants, inflammatory, fibrotic, and apoptotic genes, and histological alterations were assessed.Key findings: Examination confirmed the presence of various polyphenols in the plant extract. PCA extract administration to rats reduced the DOX-instigated elevation in CK, LDH, and triglycerides concentrations in serum and restored the histopathological changes. Similarly, PCA extract administration normalized the levels of antioxidants and pro-inflammatory markers and reduced the expression of apoptotic and fibrosis markers.Conclusion: PCA extract exhibited significant antioxidants and cardioprotective activities in rats. The cardioprotective and anti-inflammatory effects of PCA may be attributed to its high content of polyphenolics, and it acts as a promising therapeutic source against heart failure.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the neuroprotective role of Synta-66 in type-2 diabetes mellitus-induced dementia in rats. Synta-66在2型糖尿病痴呆大鼠中的神经保护作用。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-12 DOI: 10.1093/jpp/rgaf036

Ashi Mannan, Maneesh Mohan, Shareen Singh, Sonia Dhiman, Thakur Gurjeet Singh

{"title":"Investigating the neuroprotective role of Synta-66 in type-2 diabetes mellitus-induced dementia in rats.","authors":"Ashi Mannan, Maneesh Mohan, Shareen Singh, Sonia Dhiman, Thakur Gurjeet Singh","doi":"10.1093/jpp/rgaf036","DOIUrl":"https://doi.org/10.1093/jpp/rgaf036","url":null,"abstract":"Objectives: This study explores the potential inhibitory effects of Synta-66 at doses of 1 and 5 mg/kg, with a particular emphasis on the role of ORAI-I in amyloidogenesis, a common mechanism that underlies type-2 diabetes mellitus (T2DM) and Alzheimer's disease (AD).Methods: Induction of T2DM-induced AD by the high-fat diet (HFD)-Streptozotocin (STZ)-Aβ25-35 model. Assessment of behavioral parameters like polydipsia, polyphagia, Morris water maze, and passive avoidance test; biochemical estimation of glucose, insulin, oxidative stress (superoxide dismutase (SOD), glutathione (GSH), catalase (Cat), and thiobarbituric acid reactive substances (TBARS)), neuroinflammation (interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α), and nuclear factor-kappa B (NF-κβ)), Aβ level, through ELISA technique, and calcium levels via atomic absorption spectrometer.Key findings: Synta-66 (5 and 10 mg/kg) results in a reduction in food and water intake, as well as a reduction in memory impairment in the Morris water maze and passive avoidance test. Furthermore, it normalizes glucose, insulin, and antioxidant elements (SOD, GSH, and Cat) level, while decreasing TBARS levels. In addition, ELISA data demonstrated a reduction in neuroinflammation (downregulation of IL-1β, IL-6, TNF-α, and NF-κβ), Aβ accumulation, and calcium levels by Synta-66 (5 and 10 mg/kg).Conclusion: Consequently, ORAI can play a crucial role in the mediation of amyloidogenesis induced by T2DM, thereby establishing a connection between T2DM and AD. Therefore, Synta-66 has the potential to treat and prevent the progression of T2DM to AD.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the in vitro, in vivo anti-inflammatory potential of Ocimum basilicum and in silico analysis of its phytocompounds targeting COXs proteins. 揭示basilicum的体外和体内抗炎潜力，并对其靶向COXs蛋白的植物化合物进行硅分析。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-11 DOI: 10.1093/jpp/rgaf032

Nimrah Zafar, Azhar Rafique, Shabana Naz, Muhammad Muzammil Nazir, Asma Ashraf

{"title":"Unveiling the in vitro, in vivo anti-inflammatory potential of Ocimum basilicum and in silico analysis of its phytocompounds targeting COXs proteins.","authors":"Nimrah Zafar, Azhar Rafique, Shabana Naz, Muhammad Muzammil Nazir, Asma Ashraf","doi":"10.1093/jpp/rgaf032","DOIUrl":"https://doi.org/10.1093/jpp/rgaf032","url":null,"abstract":"Objectives: This study aimed to evaluate the anti-inflammatory potential of ethanolic extract of Ocimum basilicum seeds (EEOBS) through in vitro, in vivo, and in silico approaches.Methods: The in vivo anti-inflammatory activity of EEOBS was assessed using a carrageenan-induced paw edema model in Swiss albino mice, where paw thickness was measured at 1, 2, 3, 4, and 5 hours post-treatment. The in vitro anti-inflammatory potential was evaluated using a bovine serum albumin (BSA) denaturation assay at varying concentrations of EEOBS (50, 100, 250, 500, and 1000 μg/ml).Key findings: Gas chromatography-mass spectrometry (GC-MS) analysis of EEOBS revealed the presence of several bioactive phytochemicals, with 9,12,15-Octadecatrienoic acid (47.27%) and hexadecanoic acid (13.45%) as the major constituents. Histopathological analysis of mice paws showed significant restoration of the keratin and epithelium layers in treated groups compared to the control. Molecular docking analysis identified linoleic acid and 12-Z-octadecatrienoic acid as the most promising compounds, demonstrating higher binding affinity than the standard inhibitor for both cyclooxygenase proteins (COX-1: PDB ID 1EQG and COX-2: PDB ID 1CX2). Additionally, n-octadecanoic acid exhibited superior binding with COX-2 (1CX2).Conclusion: The in vitro, in vivo, and in silico findings suggest that EEOBS possesses significant anti-inflammatory potential, indicating its suitability for targeted anti-inflammatory therapies. However, further clinical trials are required to validate its therapeutic efficacy.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginsenoside Rb1 inhibits chronic stress-induced colorectal cancer via regulating glycolysis and β2-AR/CREB1 signaling pathway. 人参皂苷Rb1通过调节糖酵解和β2-AR/CREB1信号通路抑制慢性应激性结直肠癌。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-11 DOI: 10.1093/jpp/rgaf031

Wang Yao, Dong-Ming Hua, Wen-Kai Wang, Zhao-Zhou Zhang, Yun-Feng Guan, Yan Wang

{"title":"Ginsenoside Rb1 inhibits chronic stress-induced colorectal cancer via regulating glycolysis and β2-AR/CREB1 signaling pathway.","authors":"Wang Yao, Dong-Ming Hua, Wen-Kai Wang, Zhao-Zhou Zhang, Yun-Feng Guan, Yan Wang","doi":"10.1093/jpp/rgaf031","DOIUrl":"https://doi.org/10.1093/jpp/rgaf031","url":null,"abstract":"Objective: To investigate the mechanism of ginsenoside Rb1 (G-Rb1) against colorectal cancer under chronic stress.Methods: A chronic restraint stress (CRS) model and a colorectal cancer (CRC) subcutaneous xenograft model were established. Western blot analysis quantified β2-adrenergic receptor (β2-AR), cAMP response element-binding protein 1 (CREB1), and p-CREB1 expression. Additionally, glycolytic enzymes GLUT1, HK2, and PFKP were analyzed via Western blot and RT-qPCR, with glucose uptake, lactate, ATP, and stress hormone levels assessed by flow cytometry, kits, and ELISA.Key findings: Compared to the control group, the stress group exhibited increased tumor volume and mass, along with elevated expression of β2-AR, p-CREB1, and upregulated expression levels of GLUT1, HK2, and PFKP. Additionally, glucose, lactate, and epinephrine levels were higher in the stress group. In comparison to the stress group, G-Rb1 treatment demonstrated reduced tumor volume and mass, decreased p-CREB1 expression, as well as downregulated protein and mRNA levels of GLUT1, HK2, and PFKP. Glucose, lactate, and epinephrine levels also showed a reduction in the G-Rb1-treated groups.Conclusions: G-Rb1 suppresses the growth of colorectal cancer under chronic stress, potentially through downregulation of the β2-AR/CREB1 signaling pathway, thereby reducing glycolytic activity in colorectal cancer under chronic stress.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the molecular mechanism of Taohong Siwu decoction in the treatment of non-small-cell lung cancer based on network pharmacology and molecular docking. 基于网络药理学和分子对接的桃红四物汤治疗非小细胞肺癌的分子机制探索

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-09 DOI: 10.1093/jpp/rgae141

Yuan Qin, Jia-Ning Lian, Xin Chen, Feng-Yu Huang, Hai-Wen Chen, Tai-Wei Dong, Zuo-Lin Jin

{"title":"Exploring the molecular mechanism of Taohong Siwu decoction in the treatment of non-small-cell lung cancer based on network pharmacology and molecular docking.","authors":"Yuan Qin, Jia-Ning Lian, Xin Chen, Feng-Yu Huang, Hai-Wen Chen, Tai-Wei Dong, Zuo-Lin Jin","doi":"10.1093/jpp/rgae141","DOIUrl":"10.1093/jpp/rgae141","url":null,"abstract":"Objective: This study aimed to explore the mechanism of Taohong Siwu decoction (THSWD) in the treatment of non-small-cell lung cancer (NSCLC) by using comprehensive analysis.Methods: The active components and relevant targets of THSWD were analyzed by network analysis to construct the active component-target-disease network diagram. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted on the core targets by the Metascape database. Molecular docking verification was used for molecular visualization.Key findings: A total of 69 active compounds and 114 targets were filtered in lung cancer treatment with THSWD. KEGG analysis suggested that tumor necrosis factor (TNF) signaling pathway, and apoptosis pathway played critical roles. The results of molecular docking showed that populoside_qt with IL-6, baicalein with epidermal growth factor receptor (EGFR), and luteolin with MAPK8 had the strongest binding ability. Moreover, experiment validation revealed that THSWD regulated the expression of IL-6, AKT, Cyclin D1, E-cadherin, and LC3A/B, thereby inhibiting the proliferation and migration ability, promoting apoptosis, and blocking the cell cycle of NSCLC cells.Conclusions: The potential targets and molecular mechanisms of THSWD in the treatment of NSCLC were preliminarily revealed by a comprehensive analysis in this study, which will provide new ideas and methods for the study of the mechanism of traditional Chinese medicine in treating lung cancer.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"805-821"},"PeriodicalIF":2.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142836980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piperlongumine inhibits glioblastoma proliferation by inducing ferroptosis. 胡椒明通过诱导铁下垂抑制胶质母细胞瘤增殖。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-09 DOI: 10.1093/jpp/rgae148

Jianting Qiu, Fangzhou Guo, Ji Shi, Tangjun Guo, Haozhe Piao

{"title":"Piperlongumine inhibits glioblastoma proliferation by inducing ferroptosis.","authors":"Jianting Qiu, Fangzhou Guo, Ji Shi, Tangjun Guo, Haozhe Piao","doi":"10.1093/jpp/rgae148","DOIUrl":"10.1093/jpp/rgae148","url":null,"abstract":"Objectives: This study aimed to investigate the effects of Piperlongumine on Glioblastoma multiforme.Methods: The effects of Piperlongumine on the viability and proliferation of glioma cells LN229 and A172 were measured. Changes in mitochondrial structure were observed. Cell proliferative capacity was assessed using immunofluorescence. The levels of glutathione, malondialdehyde, 4-hydroxynonenal, and intracellular reactive oxygen species were detected. The levels of ferroptosis-related proteins were detected. A plasmid transfection was performed to overexpress the nuclear factor erythroid 2-related factor 2 gene; a subcutaneous tumor model was established in nude mice to observe the in vivo inhibitory effects of Piperlongumine on Glioblastoma multiforme and the recovery effect of Fer-1. The expression levels of ferroptosis-related proteins were detected using immunohistochemistry.Key findings: Piperlongumine inhibited the viability of glioma cells, as well as their proliferation. The ferroptosis inhibitors were able to restore the inhibitory effect of Piperlongumine on glioma cell proliferation. Forced overexpression of nuclear factor erythroid 2-related factor 2 partially reversed Piperlongumine-induced ferroptosis; Piperlongumine exhibited a significant inhibitory effect on Glioblastoma multiforme cells in vivo, which could be restored by Fer-1.Conclusions: Piperlongumine inhibits Glioblastoma multiforme proliferation by inducing ferroptosis in vitro and vivo model.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"822-833"},"PeriodicalIF":2.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0