The effects of Calophyllum brasiliense leaves extract and isolated compound amentoflavone: implications in the resolution of inflammation.

IF 2.8 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Larissa Benvenutti, Guilherme Moreschi Gerhardt, Ruth Meri Lucinda, Otto Maurício Santos Gerlach, Valdir Cechinel-Filho, Luiz Carlos Klein-Júnior, Nara Lins Meira Quintão, José Roberto Santin
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Abstract

Calophyllum brasiliense is employed in folk medicine as an analgesic and to treat inflammation. This study aimed to evaluate the anti-inflammatory effect of C. brasiliense leaves' methanol extract, fractions, and the isolated compound amentoflavone. In vitro fMLP-induced neutrophil chemotaxis and LPS-induced inflammatory mediator levels were assessed in neutrophils or macrophages treated or not with different concentrations of C. brasiliense extract, its fractions or amentoflavone. Their effects on inflammation resolution was assessed by evaluating the efferocytosis. The extract and its fractions (DFCB, AFCB, and MFCB) impaired neutrophil migration stimulated by the chemotactic mediator fMLP and its ability to produce and/or to release cytokines (TNF and IL-6) and NO. The increase of the apoptotic neutrophil efferocytosis was observed for cells treated with the ethyl acetate and methanol fractions, accompanied by the enhanced IL-10 levels in the supernatant and the decrease of TNF, as well. Amentoflavone, present in high concentration in ethyl acetate fraction, reduced the inflammatory mediators levels in LPS-stimulated macrophages, impaired the neutrophil chemotaxis, and enhanced the efferocytosis. The obtained data demonstrate that C. brasiliense extract presented anti-inflammatory effects by modulating neutrophil migration/activation, macrophage-dependent efferocytosis, and inflammatory mediator release, effects at least partly addressed to amentoflavone content.

嫩叶提取物和分离的复方阿门托黄酮对炎症反应的影响。
花椒在民间医学中被用作止痛剂和治疗炎症。本研究旨在评价巴西螺叶甲醇提取物、馏分及分离得到的化合物丙烯黄酮的抗炎作用。采用不同浓度的巴西螺提取物、其组分或阿门黄酮处理或未处理的中性粒细胞或巨噬细胞,观察fmlp诱导的中性粒细胞趋化性和lps诱导的炎症介质水平。通过评价红细胞增生来评价其对炎症消退的影响。提取物及其组分(DFCB、AFCB和MFCB)可抑制趋化介质fMLP刺激的中性粒细胞迁移及其产生和/或释放细胞因子(TNF和IL-6)和NO的能力。经乙酸乙酯和甲醇处理的细胞凋亡中性粒细胞efferocytosis增加,同时上清中IL-10水平升高,TNF降低。乙酸乙酯部分中高浓度的阿门黄酮可降低lps刺激的巨噬细胞中炎症介质的水平,损害中性粒细胞趋化性,增强efferocysis。所获得的数据表明,巴西螺提取物通过调节中性粒细胞迁移/激活、巨噬细胞依赖性efferocytosis和炎症介质释放而具有抗炎作用,其作用至少部分与薄荷黄酮含量有关。
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来源期刊
CiteScore
6.60
自引率
0.00%
发文量
91
审稿时长
3 months
期刊介绍: JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.
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