Journal of Pharmacy and Pharmacology最新文献_第5页

Curcuma longa L. extract and residue prevent Alzheimer's disease in mice by regulating microglia and TLR4/NF-κB signaling pathway. 姜黄提取物和残渣通过调节小胶质细胞和TLR4/NF-κB信号通路预防小鼠阿尔茨海默病。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-27 DOI: 10.1093/jpp/rgaf034

Zhihui Xu, Jianlin Li, Xiaotong Liu, Liaoyuan Liu, Weixiong Lin, Dongmei Sun, Yu Zeng

{"title":"Curcuma longa L. extract and residue prevent Alzheimer's disease in mice by regulating microglia and TLR4/NF-κB signaling pathway.","authors":"Zhihui Xu, Jianlin Li, Xiaotong Liu, Liaoyuan Liu, Weixiong Lin, Dongmei Sun, Yu Zeng","doi":"10.1093/jpp/rgaf034","DOIUrl":"https://doi.org/10.1093/jpp/rgaf034","url":null,"abstract":"Background: Curcuma longa L. (CL) is renowned for its various health benefits and has shown potential in alleviating Alzheimer's disease (AD). The post-aqueous extraction residues (CLR) may retain valuable nutritional components. The research aimed to explore their chemical composition and neuroprotective mechanism against Aβ1-42-induced AD mice.Methods: We employed UPLC-Q-Exactive/MS to characterize the chemical constituents of CL and CLR. An HPLC method was developed to quantify three predominant curcuminoids. To investigate their neuroprotective effects against Aβ1-42-induced AD mice, we assessed cognitive function using the Morris water maze and evaluated neuronal damage through histopathological examination. Molecular mechanisms were explored using immunofluorescence, ELISA, and qRT-PCR assays.Results: The study unveiled 47 and 36 compounds in CL and CLR, respectively, and eight significant differential components. HPLC analysis revealed that CLR contained substantial curcuminoids. In Aβ1-42-induced AD mice, CL and CLR improved spatial learning and memory ability, ameliorated pathological alterations in the hippocampal region, and regulated overactivated microglia. Moreover, CL and CLR inhibited the TLR4/NF-κB inﬂammatory pathway.Conclusion: CL and CLR exhibit the anti-AD effect by regulating microglia and suppressing the TLR4/NF-κB signaling pathway, which provides a scientific basis for future nutraceutical and pharmaceutical development.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of chemical and blood transition components of Xipayi Maizibizi Oral Liquid and its excitatory purine regulation-mechanism study in treating overactive bladder. 喜帕依麦子滋口服液化学成分、血运成分分析及治疗膀胱过动症的兴奋性嘌呤调节机制研究。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-27 DOI: 10.1093/jpp/rgaf011

Menglu Wang, Yang Yang, Jiamei Xie, Yuhang Du, Yige Zhao, Yongcheng An, Ziyi Shan, Changhao He, Wanxin Fu, Yan Huang, Huilin Zhang, Baosheng Zhao

{"title":"Analysis of chemical and blood transition components of Xipayi Maizibizi Oral Liquid and its excitatory purine regulation-mechanism study in treating overactive bladder.","authors":"Menglu Wang, Yang Yang, Jiamei Xie, Yuhang Du, Yige Zhao, Yongcheng An, Ziyi Shan, Changhao He, Wanxin Fu, Yan Huang, Huilin Zhang, Baosheng Zhao","doi":"10.1093/jpp/rgaf011","DOIUrl":"https://doi.org/10.1093/jpp/rgaf011","url":null,"abstract":"Objective: To explore the chemical components and blood transition components of Xipayi Maizibizi Oral Liquid (XP) as well as the efficacy and mechanism of XP in the treatment of overactive bladder (OAB).Methods: The chemical components and blood transition components were analysed. Rats were undergone bladder outlet obstruction surgery and divided into five groups. The study observed the general conditions, urodynamic argument and the bladder histopathological changes. The expression levels of ATP and P2X3 were evaluated.Results: One hundred and eighty-six chemical components were identified in XP, including 26 phenolic acids, 37 flavonoids, 7 quinones, 11 lignans and coumarins, 8 tannins, 9 alkaloids, 37 terpenoids, 32 steroids, and 19 other components. One hundred and twenty-eight components of XP were found in the blood, including 23 phenolic acids, 22 flavonoids, 3 quinones, 8 lignans and coumarins, 7 tannins, 7 alkaloids, 21 terpenoids, 20 steroids, and 17 other components. XP demonstrated effective treatment of OAB and downregulated the expression of ATP and P2X3.Conclusion: The characterization of the chemical composition and blood transition components of XP provided a foundation for further pharmacodynamic material basis and quality control of XP. XP could enhance the pathological status of rats with OAB by regulating ATP/P2X3.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full spectrum cannabis oil combined with omega-3 fish oil for neuropathic pain management: a novel therapeutic approach. 全谱大麻油与omega-3鱼油联合用于神经性疼痛管理：一种新的治疗方法。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-26 DOI: 10.1093/jpp/rgaf027

Cristina F Elorriaga, María E Olivera, Hugo Gongora Jara, Carlos H Laino

{"title":"Full spectrum cannabis oil combined with omega-3 fish oil for neuropathic pain management: a novel therapeutic approach.","authors":"Cristina F Elorriaga, María E Olivera, Hugo Gongora Jara, Carlos H Laino","doi":"10.1093/jpp/rgaf027","DOIUrl":"https://doi.org/10.1093/jpp/rgaf027","url":null,"abstract":"Objectives: Current pharmacological treatments for neuropathic pain have limited efficacy and may cause undesirable side effects. Cannabidiol (CBD)-enriched cannabis oil and omega-3 fatty acids (ω-3) have emerged as potential therapeutic options due to their analgesic and anti-inflammatory properties. This study aimed to assess the antinociceptive effects of combining CBD-enriched cannabis oil and ω-3 in rat models of acute and neuropathic pain.Methods: Using the hot plate test for acute pain and the chronic constriction injury (CCI) model for neuropathic pain, thermal and mechanical hypersensitivity were evaluated. Additionally, walking track analysis and the rotarod test assessed functional recovery of the sciatic nerve. Beyond that, the histological analysis of sciatic nerves exposed the neuropathological findings of the treatments.Key findings: The combined treatment of CBD-enriched cannabis oil and ω-3 effectively prevented thermal and mechanical hypersensitivity, while also improving motor impairment-induced peripheral neuropathy. Finally, combination treatment showed a protective effect against degeneration resulting from CCI.Conclusions: These findings underscore the potential of CBD-enriched cannabis oil and ω-3 as a novel therapeutic approach for neuropathic pain management, offering promising implications for future research and clinical practice.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential applications of nanocomposites in 3D-printed drug delivery systems. 纳米复合材料在3d打印给药系统中的潜在应用。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-23 DOI: 10.1093/jpp/rgaf028

Marwan Algellay, Satyajit D Sarker, Matthew Roberts, Lucy A Bosworth, Touraj Ehtezazi

{"title":"The potential applications of nanocomposites in 3D-printed drug delivery systems.","authors":"Marwan Algellay, Satyajit D Sarker, Matthew Roberts, Lucy A Bosworth, Touraj Ehtezazi","doi":"10.1093/jpp/rgaf028","DOIUrl":"https://doi.org/10.1093/jpp/rgaf028","url":null,"abstract":"Additive manufacturing is a renowned technology for producing three-dimensional objects, based on ceramic, metal, and plastic materials for different applications. This review examines and provides a perspective on using nanomaterials along with biopolymeric matrices for 3D printing (3DP) with potential applications in pharmaceutical dosage forms. Many 3DP methods have been developed for the formulation of drug delivery systems, including stereolithography, fused deposition modelling (FDM), selective laser sintering, and bioprinting through droplet- or extrusion-assisted techniques. Polymeric drug-loaded nanocapsules regulated the drug release profiles from 3D-printed tablets with faster drug release from 50% infill tablets. Also, incorporating nanomaterials/micro-ribbons significantly changed the mechanical and flow properties of polymers used in 3DP. For example, the addition of 1% w/w chitosan micro-ribbons to poly-vinyl alcohol powder improved filament mechanical properties for FDM 3DP in terms of flexibility and stiffness, with enhanced disintegration time of 3D-printed oral films. Berberine nanoparticles were integrated into a biodegradable and biocompatible 3D-printed pill, which facilitated sustained drug release and improved gastrointestinal absorption. Furthermore, nanocrystals enhanced the solubility of 3D-printed oral films. In conclusion, nanocomposites improved 3D-printed drug delivery systems in different aspects such as mechanical strength, solubility, and drug release profiles.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gegen Qinlian Decoction protects kidney in diabetic rats by improving intestinal barrier and regulating intestinal microbiota. 葛根芩连汤通过改善肠道屏障和调节肠道菌群对糖尿病大鼠肾脏的保护作用。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-20 DOI: 10.1093/jpp/rgaf030

Xinyu Zhang, Qing He, Chenxu Zhang, Zhangxin Ji, Dongmei Yang, Xueyang Wang, Conghui Liu, Chuanqi Zhang, Jingjing Yuan, Na Xu, Jun Chu

{"title":"Gegen Qinlian Decoction protects kidney in diabetic rats by improving intestinal barrier and regulating intestinal microbiota.","authors":"Xinyu Zhang, Qing He, Chenxu Zhang, Zhangxin Ji, Dongmei Yang, Xueyang Wang, Conghui Liu, Chuanqi Zhang, Jingjing Yuan, Na Xu, Jun Chu","doi":"10.1093/jpp/rgaf030","DOIUrl":"https://doi.org/10.1093/jpp/rgaf030","url":null,"abstract":"Objectives: To investigate the renoprotective effects of Gegen Qinlian Decoction (GQD) in Diabetes mellitus (DM) rats via the intestinal barrier and microbiota.Methods: GQD was analyzed by UPLC. STZ-induced DM rat models and antibiotic-induced sterile DM rat models were established, and fecal microbiota transplantation was performed in the latter. Renal function, oxidative stress, serum inflammatory factors, and pathological alterations were assessed. Intestinal cells and tight junction were observed by transmission electron microscopy. Inflammatory factors in the colon and tight junction protein expression were evaluated. The gut microbiota and its abundance were assessed by 16sRNA sequencing.Key findings: Four components were determined in the GQD, including puerarin, baicalin, berberine, and liquiritin. After GQD treatment, Scr and BUN were reduced, renal pathological changes were attenuated, intestinal cell swelling was reduced, intestinal tight junctions were improved, and GQD modulated the intestinal microbiota. Furthermore, a fecal bacterial solution containing GQD reduced renal lesions, improved intestinal tight junctions, and regulated intestinal microbiota in DM rats.Conclusions: GQD regulated the intestinal microbiota of DM rats, reduced intestinal inflammation, and repaired the intestinal barrier, thus reducing the burden on the kidneys, and exerting a protective effect on the kidneys of DM rats.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ending nuclear weapons, before they end us†. 在核武器终结我们之前终结它们。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-19 DOI: 10.1093/jpp/rgaf026

Kamran Abbasi, Parveen Ali, Virginia Barbour, Marion Birch, Inga Blum, Peter Doherty, Andy Haines, Ira Helfand, Richard Horton, Kati Juva, Jose F Lapena, Robert Mash, Olga Mironova, Arun Mitra, Carlos Monteiro, Elena N Naumova, David Onazi, Tilman Ruff, Peush Sahni, James Tumwine, Carlos Umaña, Paul Yonga, Chris Zielinski

引用次数: 0

Validating the antidiabetic potential of Nakima (Tupistra clarkei Hook.f.), a traditional food from eastern Himalayan region, through network pharmacology and in vivo experimentation. 通过网络药理学和体内实验验证喜马拉雅东部地区传统食物Nakima （Tupistra clarkei Hook.f.）的抗糖尿病潜力。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-07 DOI: 10.1093/jpp/rgaf014

Sutapa Datta, Soumita Bhattacharjee, Supriyo Ghosh, Amlan Jyoti Ghosh, Tilak Saha, Arnab Sen

{"title":"Validating the antidiabetic potential of Nakima (Tupistra clarkei Hook.f.), a traditional food from eastern Himalayan region, through network pharmacology and in vivo experimentation.","authors":"Sutapa Datta, Soumita Bhattacharjee, Supriyo Ghosh, Amlan Jyoti Ghosh, Tilak Saha, Arnab Sen","doi":"10.1093/jpp/rgaf014","DOIUrl":"https://doi.org/10.1093/jpp/rgaf014","url":null,"abstract":"Objective: To explore and understand the antidiabetic activity of Tupistra clarkei Hook.f. inflorescence, providing a scientific explanation to the ethnomedicinal properties.Methods: The constituents of the plant were determined through GC-MS analysis, which were used for target prediction and network pharmacology to understand how the plant regulates hyperglycaemia and other diabetes complications. These properties were validated in vivo along with further assessment of the antioxidant potential of the plant, both in vitro and in vivo.Key findings: The plant showed good phenol-flavonoid content, and antioxidant potential both in vitro and in vivo. GC-MS analysis identified 24 constituents of the plant. In silico analysis showed their ability to target 166 proteins that are associated with pathways in controlling hyperglycaemia and other diabetic consequences, protection of pancreatic tissue, insulin secretion, and insulin resistance. This was reflected in the in vivo experiment where T. clarkei showed ability to reduce FBG, LDL-C, VLDL-C levels, improve the levels of HDL-C, and also facilitate reversal of damage in pancreatic islets.Conclusion: Our study validated the antidiabetic potential Tupistra clarkei inflorescence in the in silico and in vivo assessment, and has proved to have good antioxidant activity and potential against diabetes. However, further clinical trials are essential.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bruceine E attenuates hepatic steatosis through modulation of PI3K/AKT/NFκB signalling pathway. 麻菜碱E通过调节PI3K/AKT/NFκB信号通路减轻肝脂肪变性。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-07 DOI: 10.1093/jpp/rgaf016

Farahdina Man, Neti Eka Jayanti, Chiuan Yee Leow, Chee-Yan Choo

{"title":"Bruceine E attenuates hepatic steatosis through modulation of PI3K/AKT/NFκB signalling pathway.","authors":"Farahdina Man, Neti Eka Jayanti, Chiuan Yee Leow, Chee-Yan Choo","doi":"10.1093/jpp/rgaf016","DOIUrl":"https://doi.org/10.1093/jpp/rgaf016","url":null,"abstract":"Objectives: This study aims to establish the effect of bruceine E in attenuating nonalcoholic steatohepatitis (NASH) through the PI3K/AKT/NFκB pathway.Methods: High-fat-diet (HFD) male Wistar rats were orally administered with glibenclamide (20 mg/kg) or bruceine E (400, 800, or 1600 µg/kg) for 4 weeks. After 4 weeks of treatment, blood serum was analysed for liver markers. Liver histology was used to identify the degree of inflammation. The liver tissue was evaluated on the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and inflammatory genes (nuclear factor-kappa B [NFκB], tumor necrosis factor alpha [TNFα], interleukin-6 [IL6], and interleukin-10 [IL10]) and protein expressions.Key findings: The alanine transferase and aspartate transferase were reduced in HFD rats administered orally with bruceine E. In liver histology, steatosis, ballooning, and lobular inflammation were alleviated in bruceine E-treated HFD rats. The PI3K/AKT genes and proteins were activated while the inflammatory genes and protein expressions were suppressed in the bruceine E-treated HFD rats showing improvement towards insulin resistance (IR), liver steatosis, and inflammation.Conclusions: In conclusion, bruceine E attenuated NASH through activation of the PI3K/AKT/NFκB inflammation pathway and may further delay the progression of NASH to hepatocellular carcinoma .","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of quetiapine-loaded microneedles-based transdermal patches for improved drug delivery. 奎硫平微针透皮贴剂的开发与特性研究。

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-07 DOI: 10.1093/jpp/rgaf013

Maryam Itbar, Muhammad Imran Khan, Muhammad Furqan Akhtar, Zulcaif Ahmad, Muhammad Farhan Sohail, Asadullah Madni, Alia Erum, Aslam Khan, Ahsan Ali, Muhammad Naeem Qaisar

{"title":"Development and characterization of quetiapine-loaded microneedles-based transdermal patches for improved drug delivery.","authors":"Maryam Itbar, Muhammad Imran Khan, Muhammad Furqan Akhtar, Zulcaif Ahmad, Muhammad Farhan Sohail, Asadullah Madni, Alia Erum, Aslam Khan, Ahsan Ali, Muhammad Naeem Qaisar","doi":"10.1093/jpp/rgaf013","DOIUrl":"https://doi.org/10.1093/jpp/rgaf013","url":null,"abstract":"Objectives: This study was executed to prepare and characterize quetiapine (antipsychotic drug)-loaded microneedles-based transdermal patch for improved drug delivery.Methods: This study was executed to develop microneedles-based transdermal patches (MNS) for quetiapine delivery. Eight MNS patches loaded with quetiapine (MNS1-MNS8) were fabricated using varying concentrations of sodium alginate and carboxymethyl cellulose. First four MNS patches (MNS1, MNS2, MNS3, and MNS4) were prepared by keeping sodium alginate concentration constant (6%) and increasing CMC concentration from 3% to 6%, whereas MNS5, MNS6, MNS7, and MNS8 were developed using sodium alginate to CMC concentrations 7:3, 7:4, 8:3, and 8:4, respectively. Solvent casting technique was opted for preparation of MNS patches. MNS were characterized for thickness, folding endurance, insertion capacity, drug content, morphology, and ex-vivo permeation profile using Wistar rat skin.Key findings: FTIR studies revealed the compatibility of quetiapine with formulation composites. Thickness and folding endurance was ranged in between 0.53-0.55 mm and 25-264, respectively. SEM of optimized patch showed sharp pointed needles. Ex-vivo permeation studies showed percent drug release of 84.34% from MNS1 after 48 h.Conclusions: The overall findings of study proposed that the quetiapine-loaded MNS patches hold promise for the improved transdermal delivery of quetiapine.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DST regulates cisplatin resistance in colorectal cancer via PI3K/Akt pathway. DST 通过 PI3K/Akt 通路调节结直肠癌的顺铂耐药性

IF 2.8 4区医学

Journal of Pharmacy and Pharmacology Pub Date : 2025-05-02 DOI: 10.1093/jpp/rgae104

Jianwei Yu, Xueqiong Deng, Xueqin Lin, Li Xie, Sisi Guo, Xiaoliang Lin, Dong Lin

{"title":"DST regulates cisplatin resistance in colorectal cancer via PI3K/Akt pathway.","authors":"Jianwei Yu, Xueqiong Deng, Xueqin Lin, Li Xie, Sisi Guo, Xiaoliang Lin, Dong Lin","doi":"10.1093/jpp/rgae104","DOIUrl":"10.1093/jpp/rgae104","url":null,"abstract":"Objectives: Dystonin (DST), a potential tumor suppressor gene, plays a crucial role in regulating cancer cell proliferation and resistance to chemotherapy. However, DST's specific role in colorectal cancer (CRC) has not been thoroughly investigated, and this study aims to elucidate its molecular role in modulating cisplatin (DDP) resistance in CRC.Methods: DST expression was analyzed in CRC tumors, DDP-resistant CRC tissues, paracancer tissues, and normal tissues. Lentiviral overexpression and shRNA knockdown were conducted in advanced CRC and DDP-resistant cell lines to assess cell viability, apoptosis, invasion, migration, proliferation, and angiogenesis. Xenograft mouse models studied DST's impact on CRC tumor growth and DDP resistance in vivo.Results: DST expression was significantly reduced in CRC tumor and DDP-resistant CRC tissues compared to paracancer and normal tissues (P < .001). Upregulating DST inhibited CRC and DDP-resistant cell viability, proliferation, invasion, and migration while promoting apoptosis. DST overexpression also reduced angiogenesis and attenuated DDP-induced cytotoxicity in CRC cells. Mechanistically, DST upregulation suppressed DDP resistance in CRC cells via the PI3K/Akt signaling pathway. DST upregulation reduced CRC tumor growth and mitigated DDP resistance, in vivo.Conclusion: DST plays a crucial role in limiting CRC progression and overcoming DDP resistance, suggesting potential for targeted CRC therapies.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":"698-713"},"PeriodicalIF":2.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0