Thymol suppressed tumor growth in vitro and in vivo through inducing calcium overload in colorectal cancer.

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of Pharmacy and Pharmacology Pub Date : 2025-06-06 DOI:10.1093/jpp/rgaf040

Hao Lin, Zongjun Chen, Weizhong Yang, Xianwei Wang

{"title":"Thymol suppressed tumor growth in vitro and in vivo through inducing calcium overload in colorectal cancer.","authors":"Hao Lin, Zongjun Chen, Weizhong Yang, Xianwei Wang","doi":"10.1093/jpp/rgaf040","DOIUrl":null,"url":null,"abstract":"Background: Thymol, a bioactive phenolic compound, has proven to possess multiple anti-cancer activities, yet the function and underlying mechanism in colorectal cancer (CRC) remain unclear.Objectives: To shed light on the possible therapeutic effects of thymol in CRC based on calcium homeostasis regulation, and seek to explore the molecular pathways of calcium overload in the thymol-induced anti-CRC activity.Methods: The effects of thymol on cell proliferation, viability, apoptosis, anti-inflammatory effects, and calcium overload phenotype were investigated in HCT116 and CT26 cells. In addition, the in vivo therapeutic efficacies of thymol on CT26 xenograft tumor were also researched. Furthermore, molecular mechanisms of thymol-induced calcium overload were detected by Western blot, RT-qPCR, and immunofluorescence assays.Results: We demonstrated that thymol significantly inhibited the proliferation, viability, and induced apoptosis of HCT116 and CT26 cells. And, thymol suppressed the secretion of inflammatory factors. Furthermore, thymol promoted cell damage mediated by increased mitochondrial membrane potential in both two cells. In addition, thymol triggered the energy metabolism inhibition induced by calcium overload in HCT116 and CT26 cells. Besides, in vivo experiments based on CT26 xenograft tumor model also validated the positive anti-CRC activities.Conclusions: Thymol inhibits CRC partially through inducing calcium overload, which provides an innovative solution for developing anti-CRC drugs.","PeriodicalId":16960,"journal":{"name":"Journal of Pharmacy and Pharmacology","volume":" ","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pharmacy and Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/jpp/rgaf040","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Thymol, a bioactive phenolic compound, has proven to possess multiple anti-cancer activities, yet the function and underlying mechanism in colorectal cancer (CRC) remain unclear.

Objectives: To shed light on the possible therapeutic effects of thymol in CRC based on calcium homeostasis regulation, and seek to explore the molecular pathways of calcium overload in the thymol-induced anti-CRC activity.

Methods: The effects of thymol on cell proliferation, viability, apoptosis, anti-inflammatory effects, and calcium overload phenotype were investigated in HCT116 and CT26 cells. In addition, the in vivo therapeutic efficacies of thymol on CT26 xenograft tumor were also researched. Furthermore, molecular mechanisms of thymol-induced calcium overload were detected by Western blot, RT-qPCR, and immunofluorescence assays.

Results: We demonstrated that thymol significantly inhibited the proliferation, viability, and induced apoptosis of HCT116 and CT26 cells. And, thymol suppressed the secretion of inflammatory factors. Furthermore, thymol promoted cell damage mediated by increased mitochondrial membrane potential in both two cells. In addition, thymol triggered the energy metabolism inhibition induced by calcium overload in HCT116 and CT26 cells. Besides, in vivo experiments based on CT26 xenograft tumor model also validated the positive anti-CRC activities.

Conclusions: Thymol inhibits CRC partially through inducing calcium overload, which provides an innovative solution for developing anti-CRC drugs.

查看原文本刊更多论文

百里香酚通过诱导结直肠癌钙超载抑制肿瘤生长。

背景：百里香酚是一种具有生物活性的酚类化合物，已被证实具有多种抗癌活性，但其在结直肠癌中的作用及其机制尚不清楚。目的：探讨百里香酚通过钙稳态调节对结直肠癌的可能治疗作用，探索百里香诱导的抗结直肠癌活性中钙超载的分子途径。方法：研究百里香酚对HCT116和CT26细胞增殖、活力、凋亡、抗炎和钙超载表型的影响。此外，还研究了百里香酚对CT26异种移植瘤的体内治疗效果。此外，通过Western blot、RT-qPCR和免疫荧光法检测胸腺醇诱导钙超载的分子机制。结果：百里香酚能显著抑制HCT116和CT26细胞的增殖、活性并诱导细胞凋亡。百里香酚抑制炎症因子的分泌。此外，百里香酚促进了两种细胞线粒体膜电位升高介导的细胞损伤。此外，百里香酚可触发HCT116和CT26细胞钙超载诱导的能量代谢抑制。此外，基于CT26异种移植肿瘤模型的体内实验也验证了其抗crc阳性活性。结论：百里香酚通过诱导钙超载部分抑制结直肠癌，为开发抗结直肠癌药物提供了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Pharmacy and Pharmacology 医学-药学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.